76,569 results match your criteria Blood cells molecules & diseases[Journal]


Aberrant hyperactivation of cytotoxic T-cell as a potential determinant of COVID-19 severity.

Int J Infect Dis 2020 May 31. Epub 2020 May 31.

Department of Internal Medicine, Seoul National University College of Medicine, Seoul 03080, Republic of Korea. Electronic address:

Objectives: We hypothesized that immune response may contribute to progression of coronavirus disease-19 (COVID-19) at the second week of illness. Therefore, we compared cell-mediated immune (CMI) responses between severe and mild COVID-19 cases.

Methods: We examined peripheral blood mononuclear cells of laboratory-confirmed COVID-19 patients from their first and third weeks of illness. Read More

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http://dx.doi.org/10.1016/j.ijid.2020.05.106DOI Listing

A new acetylcholinesterase allosteric site responsible for binding voluminous negatively charged molecules - the role in the mechanism of AChE inhibition.

Eur J Pharm Sci 2020 May 31:105376. Epub 2020 May 31.

Institute of Chemistry, Technology and Metallurgy, National Institute, University of Belgrade, Njegoševa 12, Belgrade, Serbia. Electronic address:

Acetylcholinesterase (AChE) inhibitors are important in the treatment of neurodegenerative diseases. Two inhibitors, 12-tungstosilicic acid (WSiA) and 12-tungstophosphoric acid (WPA), which have polyoxometalate (POM) type structure, have been shown to inhibit AChE activity in nM concentration. Circular dichroism and tryptophan fluorescence spectroscopy demonstrated that the AChE inhibition was not accompanied by significant changes in the secondary structure of the enzyme. Read More

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http://dx.doi.org/10.1016/j.ejps.2020.105376DOI Listing

Early diagnosis of breast and ovarian cancers by body fluids circulating tumor-derived exosomes.

Cancer Cell Int 2020 24;20:187. Epub 2020 May 24.

Department of Pathology, School of Veterinary Medicine, Shahrekord University, Shahrekord, Iran.

Exosomes (EXs) are small extracellular vesicles, a size range of 40-100 nm in diameter, actively secreted by most eukaryotic cells into surrounding body fluids like blood, saliva, urine, bile, breast milk and etc. These endosomal-derived vesicles mediate cell-cell communication between various cell populations through transmitting different signaling molecules such as lipids, proteins, and nucleic acids, and participate in a wide range of physiological and pathological body processes. Tumor-derived EXs (TDEs) are vehicles for intercellular communications by transferring bioactive molecules; they deliver oncogenic molecules and contain different molecular cargoes compared to EXs delivered from normal cells, therefore, they can be used as non-invasive invaluable biomarkers for early diagnosis and prognosis of most cancers, including breast and ovarian cancers. Read More

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http://dx.doi.org/10.1186/s12935-020-01276-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7247259PMC

Development of a human in vitro blood-brain tumor barrier model of diffuse intrinsic pontine glioma to better understand the chemoresistance.

Fluids Barriers CNS 2020 Jun 2;17(1):37. Epub 2020 Jun 2.

Laboratoire de la Barrière Hémato-Encéphalique (LBHE), Univ. Artois, UR 2465, 62300, Lens, France.

Background: Pediatric diffuse intrinsic pontine glioma (DIPG) represents one of the most devastating and lethal brain tumors in children with a median survival of 12 months. The high mortality rate can be explained by the ineligibility of patients to surgical resection due to the diffuse growth pattern and midline localization of the tumor. While the therapeutic strategies are unfortunately palliative, the blood-brain barrier (BBB) is suspected to be responsible for the treatment inefficiency. Read More

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http://dx.doi.org/10.1186/s12987-020-00198-0DOI Listing

Decidual CD8T cells exhibit both residency and tolerance signatures modulated by decidual stromal cells.

J Transl Med 2020 Jun 1;18(1):221. Epub 2020 Jun 1.

Laboratory for Reproductive Immunology, NHC Key Lab of Reproduction Regulation (Shanghai Institute of Planned Parenthood Research), Shanghai Key Laboratory of Female Reproductive Endocrine Related Diseases, Hospital of Obstetrics and Gynecology, Fudan University Shanghai Medical College, Shanghai, People's Republic of China.

Background: During early pregnancy, tolerance of the semi-allogeneic fetus necessitates comprehensive modifications of the maternal immune system. How decidual CD8T (CD8dT) cells balance maternal tolerance of the fetus with defense from invading pathogens remains undefined.

Methods: We investigated the distribution patterns of CD8T cells and their heterogeneity in paired peripheral blood and decidual tissue in the first trimester of pregnancy using flow cytometry and mRNA-Seq. Read More

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http://dx.doi.org/10.1186/s12967-020-02371-3DOI Listing

Sleep loss disrupts pericyte-brain endothelial cell interactions impairing blood-brain barrier function.

Brain Behav Immun 2020 May 30. Epub 2020 May 30.

Area of Neurosciences, Dept. Biology of Reproduction, CBS, Universidad Autónoma Metropolitana, Unidad Iztapalapa, Mexico City, Mexico. Electronic address:

Sleep loss in the rat increases blood-brain barrier permeability to circulating molecules by disrupting interendothelial tight junctions. Despite the description of the ultrastructure of cerebral microvessels and the evidence of an apparent pericyte detachment from capillary wall in sleep restricted rats the effect of sleep loss on pericytes is unknown. Here we characterized the interactions between pericytes and brain endothelial cells after sleep loss using male Wistar rats. Read More

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http://dx.doi.org/10.1016/j.bbi.2020.05.077DOI Listing

Endothelial cells produce angiocrine factors to regulate bone and cartilage via versatile mechanisms.

Theranostics 2020 1;10(13):5957-5965. Epub 2020 May 1.

Department of Orthopaedics, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, 325000, China.

Blood vessels are conduits distributed throughout the body, supporting tissue growth and homeostasis by the transport of cells, oxygen and nutrients. Endothelial cells (ECs) form the linings of the blood vessels, and together with pericytes, are essential for organ development and tissue homeostasis through producing paracrine signalling molecules, called angiocrine factors. In the skeletal system, ECs - derived angiocrine factors, combined with bone cells-released angiogenic factors, orchestrate intercellular crosstalk of the bone microenvironment, and the coupling of angiogenesis-to-osteogenesis. Read More

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http://dx.doi.org/10.7150/thno.45422DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7255007PMC

Exosome-like vesicles derived from Hertwig's epithelial root sheath cells promote the regeneration of dentin-pulp tissue.

Theranostics 2020 27;10(13):5914-5931. Epub 2020 Apr 27.

State Key Laboratory of Oral Disease, West China Hospital of Stomatology, Sichuan University, Chengdu, China.

: The formation of dentin-pulp involves complex epithelial-mesenchymal interactions between Hertwig's epithelial root sheath cells (HERS) and dental papilla cells (DPCs). Earlier studies have identified some of the regulatory molecules participating in the crosstalk between HERS and DPCs and the formation of dentin-pulp. In the present study we focused on the role of HERS-secreted exosomes in DPCs and the formation of dentin-pulp. Read More

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http://dx.doi.org/10.7150/thno.43156DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7254987PMC

Lipophilic Prodrug of Methotrexate in the Membrane of Liposomes Promotes Their Uptake by Human Blood Phagocytes.

Acta Naturae 2020 Jan-Mar;12(1):99-109

Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, 117997 Russia.

Previously, we showed that incorporation of methotrexate (MTX) in the form of a lipophilic prodrug (MTXDG) in 100-nm lipid bilayer liposomes of egg phosphatidylcholine can allow one to reduce toxicity and improve the antitumor efficiency of MTX in a mouse model of T-cell leukemic lymphoma. However, in our hemocompatibility tests , MTX liposomes caused complement (C) activation, obviously due to binding on the liposome surface and fragmentation of the C3 complement factor. In this work, we studied the interactions of MTX liposomes carrying stabilizing molecules phosphatidylinositol (PI), ganglioside GM1, or a lipid conjugate of -carboxymethylated oligoglycine (CMG) in the bilayer with subpopulations of human blood leukocytes. Read More

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http://dx.doi.org/10.32607/actanaturae.10946DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7245962PMC

CASK, the Soluble Glomerular Permeability Factor, Is Secreted by Macrophages in Patients With Recurrent Focal and Segmental Glomerulo-Sclerosis.

Front Immunol 2020 12;11:875. Epub 2020 May 12.

INSERM U1197, Villejuif, France.

Focal and segmental glomerulosclerosis (FSGS) is a frequent form of glomerulonephritis that may be caused by a soluble permeability factor and regulated by the immune system. We previously described a soluble form of calcium/calmodulin-dependent serine/threonine kinase (CASK) acting as a permeability factor in patients with recurrent FSGS (rFSGS). Here, we aimed to identify the immune cells associated with CASK secretion in patients with rFSGS. Read More

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http://dx.doi.org/10.3389/fimmu.2020.00875DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7235163PMC

Noncoding RNA 886 alleviates tumor cellular immunological rejection in host C57BL/C mice.

Cancer Med 2020 May 31. Epub 2020 May 31.

Department of Biochemistry and Molecular Biology, Capital Medical University, Beijing, China.

Non-coding RNA 886 (nc886/VTRNA2-1) is a Pol III transcript and an atypical imprinted gene. Its exact function as a negative regulator of protein kinase R establishes its connection with innate immunity. Studies have shown that nc886 silencing is closely associated with prostate cancer progression. Read More

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http://dx.doi.org/10.1002/cam4.3148DOI Listing

IL-15 and IL-23 synergize to trigger Th17 response by CLA T cells in psoriasis.

Exp Dermatol 2020 May 31. Epub 2020 May 31.

Translational Immunology, Department of Cellular Biology, Physiology and Immunology, Faculty of Biology, Universitat de Barcelona, Spain.

IL-15 has emerged as a potentially relevant target in the IL-17 response in psoriasis. However, its mechanism is poorly characterized in humans. IL-15 and IL-23 are constitutively expressed in the psoriatic lesion. Read More

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http://dx.doi.org/10.1111/exd.14113DOI Listing

Assessing the need for routine safety testing for patients being treated with dupilumab for moderate-to-severe atopic dermatitis.

Br J Dermatol 2020 Jun;182(6):e186-e209

Atopic dermatitis (also known as AD or eczema) is a common skin disease that can cause intense and persistent itching and rashes. Skin creams or ointments are not suitable or effective for some patients with moderate-to-severe AD. In these patients, oral (taken by mouth) or injected medications may be required. Read More

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http://dx.doi.org/10.1111/bjd.19065DOI Listing

The biology of vascular calcification.

Int Rev Cell Mol Biol 2020 6;354:261-353. Epub 2020 Apr 6.

Department of Life Sciences, University of Modena and Reggio Emilia, Modena, Italy.

Vascular calcification (VC), characterized by different mineral deposits (i.e., carbonate apatite, whitlockite and hydroxyapatite) accumulating in blood vessels and valves, represents a relevant pathological process for the aging population and a life-threatening complication in acquired and in genetic diseases. Read More

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http://dx.doi.org/10.1016/bs.ircmb.2020.02.007DOI Listing

The activation of NLRP3 inflammasome potentiates the immunomodulatory abilities of mesenchymal stem cells in a murine colitis model.

BMB Rep 2020 Jun 1. Epub 2020 Jun 1.

Department of Life Science in Dentistry, School of Dentistry, Pusan National University, Yangsan 50612, Republic of Korea; Dental and Life Science Institute, Pusan National University, Yangsan 50612, Republic of Korea.

Inflammasomes are cytosolic, multiprotein complexes that act at the frontline of the immune responses by recognizing pathogen- or danger-associated molecular patterns or abnormal host molecules. Mesenchymal stem cells (MSCs) have been reported to possess multipotency to differentiate into various cell types and immunoregulatory effects. In this study, we investigated the expression and functional regulation of NLR Family Pyrin Domain Containing 3 (NLRP3) inflammasome in human umbilical cord blood-derived MSCs (hUCB-MSCs). Read More

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Failure of upregulation of the cold shock Y-box protein-1 in effector T-cells of systemic lupus erythematosus patients averts cell survival.

Arthritis Rheumatol 2020 May 31. Epub 2020 May 31.

Department of Experimental Pediatrics, Otto-von-Guericke-University, 39120, Magdeburg, Germany.

Objective: The importance of the cold-shock Y-box protein-1 (YB-1) for cell homeostasis is well documented by its association with certain cancer entities. Here, we explored the role of YB-1 in T-cell homeostasis and survival, and the potential contribution of YB-1 in the pathogenesis of systemic lupus erythematosus (SLE).

Methods: We analyzed the expression of YB-1 and apoptosis by qPCR and FACS of CD4 T-cells from peripheral blood of healthy donors (n=25) and SLE patients (n=25) ex vivo as well as in vitro after a 6-day-stimulation with αCD3- or αCD3/αCD28-coupled microspheres. Read More

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http://dx.doi.org/10.1002/art.41382DOI Listing

HBV-specific CD8 T cells present higher TNFα expression but lower cytotoxicity in hepatocellular carcinoma.

Clin Exp Immunol 2020 May 31. Epub 2020 May 31.

Department of Infection Control Center, Linyi People's Hospital, Linyi, Shandong, China.

TNFα is largely regarded as a proinflammatory cytokine, but several recent researches demonstrated that TNFα could possess immunoregulatory roles with potential to suppress antitumor immunity. Chronic hepatitis B virus (HBV) infection is a major risk factor of hepatocellular carcinoma (HCC) and HBV-specific CD8 T cells could exert antitumor roles in HCC patients. Here, we found that HBV-specific CD8 T cells, both in the peripheral blood and in the tumor microenvironment, were more enriched with TNFα-expressing cells than IFNγ-expressing cells. Read More

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http://dx.doi.org/10.1111/cei.13470DOI Listing

Pretreatment with Warfarin Attenuates the Development of Ischemia/Reperfusion-Induced Acute Pancreatitis in Rats.

Molecules 2020 May 27;25(11). Epub 2020 May 27.

Department of Physiology, Faculty of Medicine, Jagiellonian University Medical College, 31-531 Cracow, Poland.

In acute pancreatitis (AP), pancreatic damage leads to local vascular injury, manifesting as endothelial damage and activation, increased vascular permeability, leukocyte rolling, sticking and transmigration to pancreatic tissue as well as activation of coagulation. Previous studies have shown that pretreatment with heparin or acenocoumarol inhibits the development of AP. The aim of the present study was to check the impact of pretreatment with warfarin, an oral vitamin K antagonist, on the development of ischemia/reperfusion-induced AP in rats. Read More

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http://dx.doi.org/10.3390/molecules25112493DOI Listing

Tailoring Chemometric Models on Blood-Derived Cultures Secretome to Assess Personalized Cancer Risk Score.

Cancers (Basel) 2020 May 26;12(6). Epub 2020 May 26.

Department of Experimental and Clinical Medicine, University Magna Graecia, 88100 Catanzaro, Italy.

The molecular protonation profiles obtained by means of an organic electrochemical transistor, which is used for analysis of molecular products released by blood-derived cultures, contain a large amount of information The transistor is based on the conductive polymer PEDOT:PSS comprising super hydrophobic SU8 pillars positioned on the substrate to form a non-periodic square lattice to measure the state of protonation on secretomes derived from liquid biopsies. In the extracellular space of cultured cells, the number of glycation products increase, driven both by a glycolysis metabolism and by a compromised function of the glutathione redox system. Glycation products are a consequence of the interaction of the reactive aldehydes and side glycolytic products with other molecules. Read More

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http://dx.doi.org/10.3390/cancers12061362DOI Listing

The Paracrine Role of Endothelial Cells in Bone Formation via CXCR4/SDF-1 Pathway.

Cells 2020 May 26;9(6). Epub 2020 May 26.

Laboratory for Bone Repair, Rambam Health Care Campus, Haifa 3109601, Israel.

Vascularization is a prerequisite for bone formation. Endothelial progenitor cells (EPCs) stimulate bone formation by creating a vascular network. Moreover, EPCs secrete various bioactive molecules that may regulate bone formation. Read More

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http://dx.doi.org/10.3390/cells9061325DOI Listing

Cloning, expression profile of the complement component C9 gene and influence of the recombinant C9 protein on peripheral mononuclear leukocytes transcriptome in half-smooth tongue sole (Cynoglossus semilaevis).

Fish Shellfish Immunol 2020 May 25. Epub 2020 May 25.

College of Life Science, Qingdao University, Qingdao, 266071, China; Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266237, China. Electronic address:

The ninth complement component (C9) is a terminal complement component (TCC) that is involved in creating the membrane attack complex (MAC) on the target cell surface. In this study, the CsC9 (C9 of Cynoglossus semilaevis) cDNA sequence was cloned and characterized. The full-length CsC9 cDNA measured 2,150 bp, containing an open reading frame (ORF) of 1,803 bp, a 5'-untranslated region (UTR) of 24 bp and a 3'-UTR of 323 bp. Read More

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http://dx.doi.org/10.1016/j.fsi.2020.05.042DOI Listing

CD36-fibrin interaction propagates FXI-dependent thrombin generation of human platelets.

FASEB J 2020 May 28. Epub 2020 May 28.

Center for Thrombosis and Hemostasis (CTH), University Medical Center, Johannes Gutenberg-University Mainz, Mainz, Germany.

Thrombin converts fibrinogen to fibrin and activates blood and vascular cells in thrombo-inflammatory diseases. Platelets are amplifiers of thrombin formation when activated by leukocyte- and vascular cell-derived thrombin. CD36 on platelets acts as sensitizer for molecules with damage-associated molecular patterns, thereby increasing platelet reactivity. Read More

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http://dx.doi.org/10.1096/fj.201903189RDOI Listing

Systematic Investigation of Polyurethane Biomaterial Surface Roughness on Human Immune Responses .

Biomed Res Int 2020 11;2020:3481549. Epub 2020 May 11.

NMI, Natural and Medical Sciences Institute at the University of Tübingen, Markwiesenstr. 55, 72770 Reutlingen, Germany.

It has been widely shown that biomaterial surface topography can modulate host immune response, but a fundamental understanding of how different topographies contribute to pro-inflammatory or anti-inflammatory responses is still lacking. To investigate the impact of surface topography on immune response, we undertook a systematic approach by analyzing immune response to eight grades of medical grade polyurethane of increasing surface roughness in three models of the human immune system. Polyurethane specimens were produced with defined roughness values by injection molding according to the VDI 3400 industrial standard. Read More

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http://dx.doi.org/10.1155/2020/3481549DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7240656PMC

Contribution of the SYK Tyrosine kinase expression to human iNKT self-reactivity.

Eur J Immunol 2020 May 27. Epub 2020 May 27.

Université de Nantes, CNRS, Inserm, CRCINA, Nantes, F-44000, France.

Invariant Natural Killer T (iNKT) cells are particular T lymphocytes, at the frontier between innate and adaptative immunities. They participate in the elimination of pathogens or tumor cells, but also in the development of allergic reactions and autoimmune diseases. From their first descriptions, the phenomenon of self-reactivity has been described. Read More

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http://dx.doi.org/10.1002/eji.201948416DOI Listing

Hydroxylated Rotenoids Selectively Inhibit the Proliferation of Prostate Cancer Cells.

J Nat Prod 2020 May 27. Epub 2020 May 27.

Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge, CB2 1EW, U.K.

Prostate cancer is one of the leading causes of cancer-related death in men. The identification of new therapeutics to selectively target prostate cancer cells is therefore vital. Recently, the rotenoids rotenone () and deguelin () were reported to selectively kill prostate cancer cells, and the inhibition of mitochondrial complex I was established as essential to their mechanism of action. Read More

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http://dx.doi.org/10.1021/acs.jnatprod.9b01224DOI Listing

Evolutionary links between intra- and extracellular acid-base regulation in fish and other aquatic animals.

J Exp Zool A Ecol Integr Physiol 2020 May 26. Epub 2020 May 26.

Department of Zoology, University of British Columbia, Vancouver, Canada.

The acid-base relevant molecules carbon dioxide (CO ), protons (H ), and bicarbonate (HCO ) are substrates and end products of some of the most essential physiological functions including aerobic and anaerobic respiration, ATP hydrolysis, photosynthesis, and calcification. The structure and function of many enzymes and other macromolecules are highly sensitive to changes in pH, and thus maintaining acid-base homeostasis in the face of metabolic and environmental disturbances is essential for proper cellular function. On the other hand, CO , H , and HCO have regulatory effects on various proteins and processes, both directly through allosteric modulation and indirectly through signal transduction pathways. Read More

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http://dx.doi.org/10.1002/jez.2367DOI Listing

How Does Ginsenoside Rh2 Mitigate Adipogenesis in Cultured Cells and Obese Mice?

Molecules 2020 May 21;25(10). Epub 2020 May 21.

Department of Human Sciences, Tennessee State University, Nashville, TN 37209, USA.

Ginsenoside Rh2, an intermediate metabolite of ginseng, but not naturally occurring, has recently drawn attention because of its anticancer effect. However, it is not clear if and how Rh2 inhibits preadipocytes differentiation. In the present study, we hypothesized that ginsenoside Rh2 attenuates adipogenesis through regulating the peroxisome proliferator-activated receptor gamma (PPAR-γ) pathway both in cells and obese mice. Read More

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http://dx.doi.org/10.3390/molecules25102412DOI Listing

Effect of peripheral blood-derived mesenchymal stem cells on macrophage polarization and Th17/Treg balance .

Regen Ther 2020 Jun 16;14:275-283. Epub 2020 May 16.

Key Laboratory of Cell Engineering of Guizhou Province and Regenerative Medicine Centre, Affiliated Hospital of Zunyi Medical University, Zunyi, Guizhou, China.

Introduction: Mesenchymal stem cells (MSCs) have always been the center of the experimental exploration of regenerative therapy together with other stem cells. Among with, peripheral blood-derived mesenchymal stem cells (PBMSCs) have been regarded as promising in clinical applications for its convenience of acquisition from peripheral blood. However, few reported experiments so far to elucidate the exact mechanisms of how PBMSC influence regeneration. Read More

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http://dx.doi.org/10.1016/j.reth.2020.03.008DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7232039PMC

Evaluation of blood-testis barrier integrity in terms of adhesion molecules in nonobstructive azoospermia.

Andrologia 2020 May 26:e13636. Epub 2020 May 26.

Department of Histology and Embryology, Faculty of Medicine, Ankara University, Ankara, Turkey.

Blood-testis barrier (BTB) is critical for maintaining fertility. The integrity of tight junctions (TJs) provides restricted permeability of BTB. The aim of this study was to evaluate the relationship between BTB and Sertoli cells. Read More

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http://dx.doi.org/10.1111/and.13636DOI Listing

Myeloma cells induce the accumulation of activated CD94low NK cells by cell-to-cell contacts involving CD56 molecules.

Blood Adv 2020 May;4(10):2297-2307

Laboratory of Immunology and Biotherapy and.

Natural killer (NK) cells represent innate effector cells potentially able to play a role during the immune response against multiple myeloma (MM). To better define the distribution and the specific properties of NK cell subsets during MM disease, we analyzed their features in the bone marrow and peripheral blood of newly diagnosed MM patients. Our findings revealed that, in both compartments, NK cells were more abundant than in healthy donors. Read More

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http://dx.doi.org/10.1182/bloodadvances.2019000953DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7252548PMC

Artificial feeder cells expressing ligands for killer cell immunoglobulin-like receptors and CD94/NKG2A for expansion of functional primary natural killer cells with tolerance to self.

Cytotherapy 2020 May 22. Epub 2020 May 22.

Department of Neurosurgery, Section Experimental Neurosurgery/Tumor Immunology, University Hospital Carl Gustav Carus, Technische Universitat Dresden, Dresden, Germany; German Cancer Consortium (DKTK), Partner Site Dresden, Dresden, Germany; German Cancer Research Center (DKFZ), Heidelberg, Germany; National Center for Tumor Diseases (NCT), Dresden, Germany. Electronic address:

Background Aims: Natural killer (NK) cells are promising cells for immunotherapy of cancer, and there are ongoing efforts to improve their ex vivo expansion to clinically relevant numbers. This study focused on the development of a C1-, C2-, Bw4 killer cell immunoglobulin-like receptor (KIR) ligand and NKG2A ligand-containing feeder cell line for autonomous expansion of functional NK cells.

Methods: PC3-derived feeder cells expressing IL-2, 4-1BBL and membrane-bound IL-15-mutDAP12 (mIL-15d) fusion protein in combinations or alone were generated and used for expansion. Read More

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http://dx.doi.org/10.1016/j.jcyt.2020.02.004DOI Listing

Ability of the immune system to fight viruses highlighted by cytometry and TCR clonotype assessments: lessons taken prior to COVID-19 virus pandemic outbreak.

Pol Arch Intern Med 2020 May 25. Epub 2020 May 25.

The intriguing aspects of SARS-CoV-2 virus are the high rate of spread and rapid progression of pneumonitis. Confronted with thousands of deaths daily worldwide, we have to build quickly the rationale behind the treatment, taking advantage from past analogues. When a new virus strikes, T-cell receptor (TCR) gamma/delta cells are in the first line of defence, activated by stress molecules and recognising some epitopes in a process that is major histocompatibility complex (MHC) independent but still specific, e. Read More

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http://dx.doi.org/10.20452/pamw.15388DOI Listing

[CD19 antigen loss after treatment of Bispecific T-cell Engager and effective response to salvage bispecific CAR-T therapy in B cell acute lymphoblastic leukemia: a case report and literature review].

Zhonghua Xue Ye Xue Za Zhi 2020 Apr;41(4):282-286

State Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Diseases, Institute of Hematology & Blood Diseases Hospital, CAMS & PUMC, Tianjin 300020, China.

To analyze the influence of CD19 isoforms to the efficacy of CD19/CD3 Bispecific T-cell Engager (BiTE) antibody, and explore the resistance mechanism of BiTE immunotherapy. Semi-quantitative RT-PCR (qRT-PCR) was used to detect the expression of CD19 mRNA isoforms before and after BiTE treatment in a patient with CD19(+) B cell acute lymphoblastic leukemia (ALL) . CD19 isoforms were analyzed by Sanger sequencing. Read More

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http://dx.doi.org/10.3760/cma.j.issn.0253-2727.2020.04.004DOI Listing

Proteomics and transcriptomics study reveals the utility of ISGs as novel molecules for early pregnancy diagnosis in dairy cows.

J Reprod Immunol 2020 May 19;140:103148. Epub 2020 May 19.

Lactation and Immuno-Physiology Laboratory, ICAR-National Dairy Research Institute, Karnal, Haryana 132001, India. Electronic address:

An early and precise diagnosis of pregnancy in cows is critical to short the calving interval and to improve their reproductive efficiency. Neutrophils are the first blood cells to sensitize the embryo in the uterus and participate in maternal recognition of pregnancy after getting induced by interferon tau (IFNτ). To study the protein abundance ratio, blood samples were collected on 0th, 10th, 18th and 36th day post-artificial insemination (AI) from crossbred Karan Fries cows. Read More

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http://dx.doi.org/10.1016/j.jri.2020.103148DOI Listing

Mast cells and complement system: Ancient interactions between components of innate immunity.

Allergy 2020 May 23. Epub 2020 May 23.

Department of Bacteriology and Immunology, Immunobiology Research Program, University of Helsinki, Helsinki, Finland.

The emergence and evolution of the complement system and mast cells (MCs) can be traced back to sea urchins and the ascidian Styela plicata, respectively. Acting as a cascade of enzymatic reactions, complement is activated through the classical (CP), the alternative (AP) and the lectin pathway (LP) based on the recognized molecules. The system's main biological functions include lysis, opsonization and recruitment of phagocytes. Read More

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http://dx.doi.org/10.1111/all.14413DOI Listing

Discovery of novel N-substituted thiazolidinediones (TZDs) as HDAC8 inhibitors: in-silico studies, synthesis, and biological evaluation.

Bioorg Chem 2020 May 15;100:103934. Epub 2020 May 15.

Department of Pharmaceutical Chemistry, Bharati Vidyapeeth's College of Pharmacy, Navi Mumbai, India. Electronic address:

Epigenetics plays a fundamental role in cancer progression, and developing agents that regulate epigenetics is crucial for cancer management. Among Class I and Class II HDACs, HDAC8 is one of the essential epigenetic players in cancer progression. Therefore, we designed, synthesized, purified, and structurally characterized novel compounds containing N-substituted TZD (P1-P25). Read More

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http://dx.doi.org/10.1016/j.bioorg.2020.103934DOI Listing

Surmounting the endothelial barrier for delivery of drugs and imaging tracers.

Atherosclerosis 2020 May 11. Epub 2020 May 11.

Amsterdam UMC, University of Amsterdam, Department of Experimental Vascular Medicine, Amsterdam Cardiovascular Sciences, Meibergdreef 9, Amsterdam, Netherlands. Electronic address:

The endothelium is crucial for maintaining vascular homeostasis and functions as a barrier between blood components and tissue. In atherosclerosis, this barrier function is impaired, which is characterized by secretion of chemoattractants and cytokines, upregulation of adhesion molecules and increased vascular permeability. This facilitates enhanced leukocyte migration through the vessel wall. Read More

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http://dx.doi.org/10.1016/j.atherosclerosis.2020.04.025DOI Listing

Engagement of people with multiple sclerosis to enhance research into the physiological effect of hyperbaric oxygen therapy.

Mult Scler Relat Disord 2020 Apr 29;43:102084. Epub 2020 Apr 29.

Institute of Biomedical and Clinical Sciences, College of Medicine & Healthcare, University of Exeter, Exeter, UK; Royal Devon and Exeter Hospital Foundation Trust, Exeter, UK.

Background: Thousands of people with multiple sclerosis (MS) have used self-administered oxygen therapy in the UK. Clinical trials have been performed, with scant evidence that people with MS have been consulted to explore how they benefit from or how to optimize this treatment. The conventional MS disease disability scores used in trials seldom reflect the effects individuals report when using oxygen therapy to treat their symptoms. Read More

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http://dx.doi.org/10.1016/j.msard.2020.102084DOI Listing

Enhanced osteogenesis of hydroxyapatite scaffolds by coating with BMP-2-loaded short polylactide nanofiber: a new drug loading method for porous scaffolds.

Regen Biomater 2020 Feb 7;7(1):91-98. Epub 2019 Nov 7.

Sichuan Provincial Laboratory of Orthopaedic Engineering, Department of Bone and Joint Surgery, Affiliated Hospital of Southwest Medical University, Luzhou 646000, China.

Porous hydroxyapatite (HA) is widely used in porous forms to assist bone defect healing. However, further improvements in biological functions are desired for meeting complex clinical situations such as impaired bone regeneration in poor bone stock. The extracellular matrix (ECM) of human tissues is characterized by nanofibrous structures and a variety of signal molecules. Read More

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http://dx.doi.org/10.1093/rb/rbz040DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7233607PMC
February 2020

The NLRP3-Mediated Neuroinflammatory Responses to CdTe Quantum Dots and the Protection of ZnS Shell.

Int J Nanomedicine 2020 6;15:3217-3233. Epub 2020 May 6.

Key Laboratory of Environmental Medicine and Engineering, Ministry of Education, School of Public Health, Southeast University, Nanjing 210009, People's Republic of China.

Introduction: Since CdTe quantum dots (QDs) are still widely considered as advanced fluorescent probes because of their far superior optical performance and fluorescence efficiency over non-cadmium QDs, it is important to find ways to control their toxicity.

Methods: In this study, the adverse effects of two cadmium-containing QDs, ie, CdTe QDs and CdTe@ZnS QDs, on the nervous system of nematode , the hippocampus of mice, and cultured microglia were measured in order to evaluate the neuroinflammation caused by cadmium-containing QDs and the potential mechanisms.

Results: Firstly, we observed that cadmium-containing QD exposure-induced immune responses and neurobehavioral deficit in nematode . Read More

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http://dx.doi.org/10.2147/IJN.S246578DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7212783PMC

Thermodynamic interference with bile acid demicelleization reduces systemic entry and injury during cholestasis.

Sci Rep 2020 May 21;10(1):8462. Epub 2020 May 21.

Department of Medicine, Mayo Clinic, Scottsdale, AZ, USA.

Bile acids (BA), with their large hydrophobic steroid nucleus and polar groups are amphipathic molecules. In bile, these exist as micelles above their critical micellar concentration (CMC). In blood at low concentrations, these exist as monomers, initiating cellular signals. Read More

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http://dx.doi.org/10.1038/s41598-020-65451-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7242474PMC

Inhibition of circulating exosomal microRNA-15a-3p accelerates diabetic wound repair.

Aging (Albany NY) 2020 May 21;12(10):8968-8986. Epub 2020 May 21.

Department of Orthopedics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.

Diabetic foot ulcers are a common complication of diabetes, and are usually incurable in the clinic. Exosomes (carriers that transfer endogenous molecules) from diabetic patients' blood have been demonstrated to suppress diabetic wound repair. In this study, we investigated the effects of circulating exosomal microRNA-15a-3p (miR-15a-3p) on diabetic wound repair. Read More

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http://dx.doi.org/10.18632/aging.103143DOI Listing

PD-L1 targeting high-affinity NK (t-haNK) cells induce direct antitumor effects and target suppressive MDSC populations.

J Immunother Cancer 2020 May;8(1)

Laboratory of Tumor Immunology and Biology, Center for Cancer Research, National Cancer Institute, Bethesda, Maryland, USA

Background: Although immune checkpoint inhibitors have revolutionized cancer treatment, clinical benefit with this class of agents has been limited to a subset of patients. Hence, more effective means to target tumor cells that express immune checkpoint molecules should be developed. For the first time, we report a novel natural killer (NK) cell line, programmed death-ligand 1 (PD-L1) targeting high-affinity natural killer (t-haNK), which was derived from NK-92 and was engineered to express high-affinity CD16, endoplasmic reticulum-retained interleukin (IL)-2, and a PD-L1-specific chimeric antigen receptor (CAR). Read More

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http://dx.doi.org/10.1136/jitc-2019-000450DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7247398PMC

PECAM-1 overexpression signifies aggressive biologic behavior of oral lichen planus - A pilot study.

Indian J Dent Res 2020 Mar-Apr;31(2):277-281

Department of Oral Pathology and Microbiology, Faculty of Dental Sciences, M S Ramaiah University of Applied Sciences, M S R Nagar, Bangalore, Karnataka, India.

Context: The etiopathogenesis of oral lichen planus (OLP) is still debatable. According to literature, many studies have illustrated OLP as a T-cell-mediated chronic autoimmune disease. Currently, there is increased evidence of chronic inflammation in OLP and its association with vascular adhesion molecules (VAMs). Read More

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http://dx.doi.org/10.4103/ijdr.IJDR_653_18DOI Listing

Differential Expression of IgM and IgD Discriminates Two Subpopulations of Human Circulating IgMIgDCD27 B Cells That Differ Phenotypically, Functionally, and Genetically.

Front Immunol 2020 6;11:736. Epub 2020 May 6.

Instituto de Genética Humana, Facultad de Medicina, Pontificia Universidad Javeriana, Bogotá, Colombia.

The origin and function of blood IgMIgDCD27 B cells is controversial, and they are considered a heterogeneous population. Previous staining of circulating B cells of healthy donors with rotavirus fluorescent virus-like particles allowed us to differentiate two subsets of IgMIgDCD27: IgM and IgM B cells. Here, we confirmed this finding and compared the phenotype, transcriptome, function, and Ig gene repertoire of these two subsets. Read More

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http://dx.doi.org/10.3389/fimmu.2020.00736DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7219516PMC

Magnetic nanoparticles double wrapped into cross-linked salep/PEGylated carboxymethyl cellulose; a biocompatible nanocarrier for pH-triggered release of doxorubicin.

Int J Biol Macromol 2020 May 8;158:994-1006. Epub 2020 May 8.

Chemical Injuries Research Center, Faculty of Pharmacy, System Biology and Poisonings Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran.

A magnetic nanocarrier was synthesized in which FeO nanoparticles were encapsulated into double layers of polysaccharide shells. The first shell, which was composed of cross-linked salep polysaccharide, contained multiple nitrogen atoms in its structure and provided numerous sites for multiple functionalization. A fluorescence dye and doxorubicin, as widely used chemotherapy agent, were easily attached to the first shell and then a second shell of PEGylated carboxymethyl cellulose enveloped the drug loaded carrier to enhance its biocompatibility and regulates the drug release behavior. Read More

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http://dx.doi.org/10.1016/j.ijbiomac.2020.05.040DOI Listing