124,365 results match your criteria Blood Coagulation & Fibrinolysis[Journal]


Preparation and characterization of crosslinked porous starch hemostatic.

Int J Biol Macromol 2020 May 25. Epub 2020 May 25.

College of Pharmaceutical Sciences, Zhejiang University of Technology, Hangzhou 310014, China.

In order to develop starch hemostatic materials with excellent hemostatic properties, the preparation of crosslinked porous starch (SPS) with sodium trimetaphosphate (STMP) as cross-linking agent was studied in this paper. When the solid-liquid ratio of porous starch (PS) was 30%, the mass ratio of cross-linking agent to starch was 0.04-1, and the SPS was crosslinked at pH 10. Read More

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http://dx.doi.org/10.1016/j.ijbiomac.2020.05.189DOI Listing

CD36-fibrin interaction propagates FXI-dependent thrombin generation of human platelets.

FASEB J 2020 May 28. Epub 2020 May 28.

Center for Thrombosis and Hemostasis (CTH), University Medical Center, Johannes Gutenberg-University Mainz, Mainz, Germany.

Thrombin converts fibrinogen to fibrin and activates blood and vascular cells in thrombo-inflammatory diseases. Platelets are amplifiers of thrombin formation when activated by leukocyte- and vascular cell-derived thrombin. CD36 on platelets acts as sensitizer for molecules with damage-associated molecular patterns, thereby increasing platelet reactivity. Read More

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http://dx.doi.org/10.1096/fj.201903189RDOI Listing

Hematological Indexes Can Be Used to Predict the Incidence of Hypothyroidism in Nasopharyngeal Carcinoma Patients after Radiotherapy.

Biomed Res Int 2020 11;2020:3860936. Epub 2020 May 11.

Institute of Cancer and Basic Medical (ICBM), Chinese Academy of Sciences, Hangzhou, China.

Background: This study explored the relationship between thyroid-associated antibodies, immune cells, and hypothyroidism to establish a predictive model for the incidence of hypothyroidism in patients with nasopharyngeal carcinoma (NPC) after radiotherapy.

Methods: A total of 170 patients with NPC treated at the Cancer Hospital of University of Chinese Academy of Sciences between January 2015 and August 2018 were included. The complete blood count, biochemical, coagulation function, immune cells, and thyroid-associated antibodies tested before radiotherapy were evaluated. Read More

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http://dx.doi.org/10.1155/2020/3860936DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7243020PMC

Blood Parameters in Treatment with Arsenic Trioxide in Acute Promyelocytic Leukemia: Systematic Review.

Int J Hematol Oncol Stem Cell Res 2020 Apr;14(2):130-139

Cancer Molecular Pathology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.

Acute promyelocytic leukemia (APL) is a subtype of acute myeloid leukemia (AML). APL is famed with some special blood coagulation disorders such as disseminated intravascular coagulation (DIC). The therapeutic methods of APL contain All Trans Retinoic Acid (ATRA), arsenic trioxide (ATO) or/and chemotherapy. Read More

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Adult Donor Blood Products as Risk Factors for Central Venous Catheter-associated Thromboembolism in Neonates: A Retrospective Case-Control Study.

J Pediatr Hematol Oncol 2020 May 26. Epub 2020 May 26.

Department of Neonatology, Charité-Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin, Germany.

This study aimed to examine whether the transfusion of donor blood products, abnormal coagulation or inflammation increase the risk of venous thromboembolism (VTE) associated with central venous catheters (CVC) in neonates. A retrospective case-control study including 25 neonates with CVC-associated VTE and tightly matched controls with CVC, but without VTE was performed. The frequency of (i) abnormal coagulation screens, (ii) increased inflammatory marker proteins before catheter insertion, or (iii) catheter-associated blood stream infection did not differ between cases and controls. Read More

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http://dx.doi.org/10.1097/MPH.0000000000001821DOI Listing

Angiopoietin-2 as a marker of endothelial activation is a good predictor factor for intensive care unit admission of COVID-19 patients.

Angiogenesis 2020 May 27. Epub 2020 May 27.

Université de Paris, Innovative Therapies in Haemostasis, INSERM, 75006, Paris, France.

Background: Coronavirus disease-2019 (COVID-19), a respiratory disease has been associated with ischemic complications, coagulation disorders, and an endotheliitis.

Objectives: To explore endothelial damage and activation-related biomarkers in COVID-19 patients with criteria of hospitalization for referral to intensive care unit (ICU) and/or respiratory worsening.

Methods: Analysis of endothelial and angiogenic soluble markers in plasma from patients at admission. Read More

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http://dx.doi.org/10.1007/s10456-020-09730-0DOI Listing

Whole exome sequencing identifies rare variants in STAB2 associated with venous thromboembolic disease.

Blood 2020 May 26. Epub 2020 May 26.

University of Michigan Medical Center, Ann Arbor, Michigan, United States.

Deep vein thrombosis and pulmonary embolism, collectively defined as venous thromboembolism (VTE), are the third leading cause of cardiovascular death in the United States. Common genetic variants conferring increased varying degrees of VTE risk have been identified by genome-wide association studies (GWAS). Rare mutations in the anticoagulant genes PROC, PROS1 and SERPINC1 result in perinatal lethal thrombosis in homozygotes and markedly increased VTE risk in heterozygotes. Read More

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http://dx.doi.org/10.1182/blood.2019004161DOI Listing

Cell Density-Dependent Fibroblast Growth Factor-2 Signaling Regulates Syndecan-4 Expression in Cultured Vascular Endothelial Cells.

Int J Mol Sci 2020 May 24;21(10). Epub 2020 May 24.

Department of Environmental Health, Faculty of Pharmaceutical Sciences, Tokyo University of Science, Noda 278-8510, Japan.

Syndecan-4 is a member of the syndecan family of transmembrane heparan sulfate proteoglycans, and is involved in cell protection, proliferation, and the blood coagulation-fibrinolytic system in vascular endothelial cells. Heparan sulfate chains enable fibroblast growth factor-2 (FGF-2) to form a complex with its receptor and to transduce the cell growth signal. In the present study, bovine aortic endothelial cells were cultured, and the intracellular signal pathways that mediate the regulation of syndecan-4 expression in dense and sparse cultures by FGF-2 were analyzed. Read More

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http://dx.doi.org/10.3390/ijms21103698DOI Listing

Platelets in Thrombo-Inflammation: Concepts, Mechanisms, and Therapeutic Strategies for Ischemic Stroke.

Hamostaseologie 2020 Jun 26;40(2):153-164. Epub 2020 May 26.

Institute of Experimental Biomedicine, University Hospital and Rudolf Virchow Center, University of Würzburg, Würzburg, Germany.

Platelets are anucleate cells known for their essential function in hemostasis and formation of thrombi under pathologic conditions. In recent years, strong evidence emerged demonstrating the critical involvement of platelets in inflammatory processes including acute ischemic stroke (AIS), which is one of the leading causes of death and disability worldwide. Recanalization of the occluded brain artery to reconstitute cerebral blood flow is the primary goal in the treatment of stroke patients. Read More

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http://dx.doi.org/10.1055/a-1151-9519DOI Listing

Procoagulation, hypercoagulation and fibrinolytic “shut down” detected with ClotPro® viscoelastic tests in COVID-19 patients.

Orv Hetil 2020 05;161(22):899-907

Általános Orvostudományi Kar, Transzplantációs és Sebészeti Klinika,Semmelweis Egyetem Budapest, Baross u. 23-25., 1083.

Introduction: International data indicate that arterial, venous and microvascular thrombosis or disseminated intravascular coagulation occur in more than 30% of hospitalized patients with COVID-19. This condition is characterized by high levels of D-dimer and fibrinogen, prolonged prothrombin time and activated partial thromboplastin time.

Method: Blood samples from three COVID-19 patients treated in a Hungarian intensive care unit were collected and analyzed with ClotPro® tests. Read More

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http://dx.doi.org/10.1556/650.2020.31870DOI Listing

Pathophysiology of the Venous Thromboembolism Risk in Preeclampsia.

Hamostaseologie 2020 May 25. Epub 2020 May 25.

Department of Haematology, Mater Misericordiae University Hospital, Dublin, Ireland.

Haemostatic derangements are a hallmark of preeclampsia and appear to favour an increased risk of venous thromboembolism. However, haemorrhagic complications have also been reported. The mechanisms underlying these competing risks remain to be fully elucidated although recent work has highlighted the role of placental factors, such as extra-cellular vesicles and inflammatory mediators, in modulating these haemostatic derangements as well as driving progression of preeclampsia. Read More

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http://dx.doi.org/10.1055/a-1162-3905DOI Listing

Successful surgical drainage with intraoperative ultrasonography for amebic liver abscess refractory to metronidazole and percutaneous drainage: a case report.

BMC Surg 2020 May 24;20(1):112. Epub 2020 May 24.

Department of Surgery, National Defense Medical College, 3-2 Namiki, Tokorozawa, Saitama, 359-8513, Japan.

Background: Metronidazole (MNZ) has been clearly established as a medication for amebic liver abscess. In uncomplicated cases, surgical drainage should be avoided. We report a case of amebic liver abscess refractory to MNZ that was successfully treated using preoperative computed tomography (CT) and percutaneous and surgical drainage with intraoperative ultrasonography (IOUS). Read More

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http://dx.doi.org/10.1186/s12893-020-00776-xDOI Listing

Identification of novel biomarkers for neonatal hypoxic-ischemic encephalopathy using iTRAQ.

Ital J Pediatr 2020 May 24;46(1):67. Epub 2020 May 24.

Department of Neonatology, Children's Hospital of Soochow University, Suzhou, China.

Background: A prompt diagnosis of HIE remains a challenge clinically. This study aimed to identify potential biomarkers of neonatal hypoxic-ischemic encephalopathy (HIE) via a novel proteomic approach, the isobaric tags for absolute and relative quantification (iTRAQ) method.

Methods: Blood samples were collected from neonates with mild (n = 4), moderate (n = 4), or severe (n = 4) HIE who were admitted to the neonatal intensive care unit of Children's Hospital of Soochow University between Oct 2015 and Oct 2017. Read More

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http://dx.doi.org/10.1186/s13052-020-00822-7DOI Listing

Anti-platelet adhesion and in situ capture of circulating endothelial progenitor cells on ePTFE surface modified with poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC) and hemocompatible peptide 1 (HCP-1).

Colloids Surf B Biointerfaces 2020 May 15;193:111113. Epub 2020 May 15.

Department of Biomedical Engineering, National Cerebral and Cardiovascular Center Research Institute, 6-1 Kishibe-Shimmachi, Suita, Osaka, 564-8565, Japan. Electronic address:

We recently reported in vitro suppression of platelet adhesion on expanded polytetrafluoroethylene (ePTFE) by surface grafting of poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC). However, this may be inadequate for long-term hemocompatibility of blood-contacting biomaterials, and it has led us to develop a strategy of circulating mononuclear cell-capture. ePTFE was treated with argon (Ar) plasma, and grafted with 2-methacryloyloxyethyl phosphorylcholine (MPC) and methacrylic acid (MAA), by glycidyl methacrylate (GMA)-anchored graft polymerization. Read More

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http://dx.doi.org/10.1016/j.colsurfb.2020.111113DOI Listing

Efficient antibacterial dextran-montmorillonite composite sponge for rapid hemostasis with wound healing.

Int J Biol Macromol 2020 May 21. Epub 2020 May 21.

State Key Laboratory of Fine Chemicals, School of Chemical Engineering, Dalian University of Technology, Dalian 116024, PR China. Electronic address:

Montmorillonite (MMT) powder, as the most effective hemostats in natural silicates, is restricted for commercial application due to its embolic effect. Until now, it's still a challenge to control the leakage of MMT and avoid its side-effects. Herein, poly aldehyde dextran (PDA)/MMT composite sponge (PM) with commendable tissue adhesion, antibacterial, and wound healing performances is developed for massive hemorrhage control. Read More

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http://dx.doi.org/10.1016/j.ijbiomac.2020.05.140DOI Listing

Effects of differences in pre-analytical processing on blood protein profiles determined with SWATH-MS.

J Proteomics 2020 May 20:103824. Epub 2020 May 20.

Department of Pharmaceutical Microbiology, School of Pharmacy, Kumamoto University, 5-1 Oe-honmachi, Chuo-ku, Kumamoto 862-0973, Japan; Faculty of Life Sciences, Kumamoto University, 5-1 Oe-honmachi, Chuo-ku, Kumamoto 862-0973, Japan; AMED-CREST, Japan Agency for Medical Research and Development, 1-7-1 Otemachi, Chiyoda, Tokyo 100-0004, Japan. Electronic address:

The purpose of the present study was to assess the differences between the human serum and plasma proteomes, and the stability of human plasma proteins under different storage conditions following blood collection, by means of SWATH-MS analysis. When we compared plasma and serum prepared immediately after blood sampling, 95.5% of 176 quantified proteins differed by less than 1. Read More

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http://dx.doi.org/10.1016/j.jprot.2020.103824DOI Listing

Influence of the Casein Composite Genotype on Milk Quality and Coagulation Properties in the Endangered Agerolese Cattle Breed.

Animals (Basel) 2020 May 20;10(5). Epub 2020 May 20.

Department of Veterinary Medicine and Animal Production, University of Naples Federico II, via Delpino 1, 80137 Naples, Italy.

The aim of this study was the characterization of CSN1S1, CSN2 and CSN3 genetic variability in Agerolese cattle, and the investigation of the effect of casein composite genotypes (CSN1S1, CSN2 and CSN3) on quality and coagulation traits of the corresponding milk. To these purposes, blood and milk from 84 cows were sampled and analysed. Allele frequencies at and revealed no Hardy-Weinberg equilibrium in the population with a prevalence of allele A for and allele B for . Read More

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http://dx.doi.org/10.3390/ani10050892DOI Listing

Use of intrapulmonary administration of thrombin in hematological malignancy patients with alveolar haemorrhage: A case series.

Medicine (Baltimore) 2020 May;99(20):e20284

Division of Hematology, Catholic Blood and Marrow Transplantation Center, Seoul St. Mary's Hospital, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea.

Introduction: Alveolar hemorrhage (AH) is characterized by the acute onset of alveolar bleeding and hypoxemia and can be fatal. Thrombin has been widely used to achieve coagulation and hemostasis. However, the efficacy of thrombin in patients with AH is unclear. Read More

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http://dx.doi.org/10.1097/MD.0000000000020284DOI Listing

A Rare Case of Immune Thrombocytopenic Purpura Secondary to COVID-19.

J Med Virol 2020 May 22. Epub 2020 May 22.

COVID-19 Research Center, Institute of Laboratory Medicine, Jinling Hospital, Nanjing University School of Medicine, Nanjing, Jiangsu, 210002, China.

Coronavirus Disease 2019 (COVID-19) is a newly emerging infectious disease caused by a novel coronavirus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). After its first occurrence in Wuhan of China from December 2019, COVID-19 rapidly spread around the world. Studies have shown that coagulation dysfunction is one of the important reason causing death of COVID-19 patients and appeared in many patients. Read More

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http://dx.doi.org/10.1002/jmv.26051DOI Listing

Thromboinflammatory mechanisms in sickle cell disease - challenging the hemostatic balance.

Haematologica 2020 May 21. Epub 2020 May 21.

Hematology Center, University of Campinas, UNICAMP, Campinas-SP, Brazil.

Sickle cell disease (SCD) is an inherited hemoglobinopathy that is caused by the presence of abnormal hemoglobin S (HbS) in red blood cells, leading to alterations in red cell properties and shape, as the result of HbS dexoygenation and subsequent polymerization. SCD pathophysiology is characterized by chronic inflammatory processes, triggered by hemolytic and vaso-occlusive events, which lead to the varied complications, organ damage and elevated mortality seen in individuals with the disease. In association with activation of the endothelium and leukocytes, hemostatic alterations and thrombotic events are well-documented in SCD. Read More

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http://dx.doi.org/10.3324/haematol.2019.239343DOI Listing

Transfusional optimization in liver transplant using viscoelastic test guided therapy.

Rev Esp Anestesiol Reanim 2020 May 18. Epub 2020 May 18.

Unidad de Cirugía Hepatobiliar y Trasplante Hepático, Hospital Universitario Cruces, Baracaldo, Vizcaya, España.

Background: Assess the reduction of packed red blood cells (PRBCs) transfusion in liver transplantation (LT) after the introduction of the thromboelastometry as intraoperative coagulation monitor.

Methods: We conducted a retrospective cohort study (n=92), randomized into two groups: groupA (control), in whom transfusion therapy was based on conventional laboratory tests (CLT), and groupB (ROTEM), whose blood transfusion was performed as protocolized algorithms, guided by thromboelastometry (ROTEM). We analyzed packed red blood cells (PRBCs) units, transfused units of fresh frozen plasma (FFP), platelets units, fibrinogen and tranexamic acid. Read More

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http://dx.doi.org/10.1016/j.redar.2020.01.015DOI Listing

Thromboelastometry Variables in Neonates with Perinatal Hypoxia.

Semin Thromb Hemost 2020 May 21. Epub 2020 May 21.

Neonatal Department, National and Kapodistrian University of Athens School of Medicine, Aretaieion Hospital, Athens, Greece.

Perinatal hypoxia is associated with an increased risk of coagulation disorders by enhancing the consumption of platelets and some clotting factors due to the associated severe hypoxemia, acidemia, and compromised oxygen and blood supply to the neonatal liver and bone marrow. Thromboelastometry (TEM), which estimates the dynamics of blood coagulation, may represent an attractive tool for studying the coagulation status of these neonates. We aimed at assessing the hemostatic profile of neonates with perinatal hypoxia using the standard extrinsically activated TEM (ex-TEM) assay. Read More

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http://dx.doi.org/10.1055/s-0040-1709473DOI Listing

Anti-Clogging mechanisms of a motion-activated chest tube patency maintenance system: Histology and high-speed camera assessment.

Artif Organs 2020 May 21. Epub 2020 May 21.

Department of Biomedical Engineering, Lerner Research Institute, Cleveland Clinic, Cleveland, Ohio, 44195, USA.

Objective: The motion-activated system (MAS) employs vibration to prevent intraluminal chest tube clogging. We evaluated the intraluminal clot formation inside chest tubes using high-speed camera imaging and post-explant histology analysis of thrombus.

Methods: The chest tube clogging was tested (MAS vs. Read More

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http://dx.doi.org/10.1111/aor.13740DOI Listing

The Study On Two-Way Adjustment Mechanism of Rhei Radix et Rhizoma for Cardiovascular Diseases.

Comb Chem High Throughput Screen 2020 May 21. Epub 2020 May 21.

School of Pharmacy, Shaanxi University of Chinese Medicine, Xianyang, Shaanxi. China.

Aim And Objective: Myocardial infarction, cerebral infarction and other diseases caused by vascular obstruction have always jeopardized human life and health. More and more reports indicate that Rhei Radix et Rhizoma has a good clinical effect in the prevention and treatment of cardiovascular disease. Owing to the complexity of herbal medicine, the pharmacodynamic mechanism of Rhei Radix et Rhizoma is still not clear. Read More

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http://dx.doi.org/10.2174/1386207323666200521120308DOI Listing

Point-of-care testing of coagulation in patients treated with edoxaban.

J Thromb Thrombolysis 2020 May 20. Epub 2020 May 20.

Department of Neurology & Stroke, and Hertie Institute for Clinical Brain Research, Eberhard-Karls University, Tübingen, Tübingen, Germany.

Edoxaban, alongside other direct oral anticoagulants (DOAC), is increasingly used for prevention of thromboembolism, including stroke. Despite DOAC therapy, however, annual stroke rate in patients with atrial fibrillation remains 1-2%. Rapid exclusion of relevant anticoagulation is necessary to guide thrombolysis or reversal therapy but, so far, no data exists on the effect of edoxaban on available point-of-care test systems (POCT). Read More

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http://dx.doi.org/10.1007/s11239-020-02143-2DOI Listing

Repeated dose (90 days) oral toxicity study of ursolic acid in Han-Wistar rats.

Toxicol Rep 2020 8;7:610-623. Epub 2020 May 8.

Department of Anatomy and Developmental Biology, Monash University, Melbourne, 3168, Australia.

Background: Ursolic acid (UA) has been used in alternative medicine for decades, and there has been a great interest in its medicinal properties. Despite this increased interest, a detailed long-term toxicity study has not been performed. The objective of this study was to determine the long-term toxic effect of UA on clinical chemistry, haematology, coagulation, pathology/morphology, behaviour and motor skills in rats. Read More

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http://dx.doi.org/10.1016/j.toxrep.2020.04.005DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7229404PMC

Integration of transcriptome-wide association study and messenger RNA expression profile to identify genes associated with osteoarthritis.

Bone Joint Res 2020 Mar 16;9(3):130-138. Epub 2020 May 16.

Key Laboratory of Trace Elements and Endemic Diseases of National Health and Family Planning Commission, School of Public Health, Health Science Center, Xi'an Jiaotong University, Xi'an, China.

Aims: Osteoarthritis (OA) is the most prevalent joint disease. However, the specific and definitive genetic mechanisms of OA are still unclear.

Methods: Tissue-related transcriptome-wide association studies (TWAS) of hip OA and knee OA were performed utilizing the genome-wide association study (GWAS) data of hip OA and knee OA (including 2,396 hospital-diagnosed hip OA patients versus 9,593 controls, and 4,462 hospital-diagnosed knee OA patients versus 17,885 controls) and gene expression reference to skeletal muscle and blood. Read More

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http://dx.doi.org/10.1302/2046-3758.93.BJR-2019-0137.R1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7229301PMC

Plasmapheresis in Sepsis-induced Thrombotic Microangiopathy: A Case Series.

Indian J Crit Care Med 2020 Mar;24(3):195-199

Department of Nephrology, Kasturba Medical College, Mangalore, Manipal Academy of Higher Education, Manipal, India.

Introduction: Cytokines and granulocyte elastase produced in sepsis cleave a disintegrin and metalloprotease with thrombospondin type I motif 13 (ADAMTS13) and deplete its levels. By this mechanism, sepsis results in microangiopathic hemolytic anemia (MAHA) with thrombocytopenia. Hence, the hypothesis is that plasmapheresis may help in sepsis-induced thrombotic microangiopathy (sTMA), by removing the factors responsible for low levels of ADAMTS13. Read More

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http://dx.doi.org/10.5005/jp-journals-10071-23374DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7225771PMC

Magnetic nanoparticles double wrapped into cross-linked salep/PEGylated carboxymethyl cellulose; a biocompatible nanocarrier for pH-triggered release of doxorubicin.

Int J Biol Macromol 2020 May 8;158:994-1006. Epub 2020 May 8.

Chemical Injuries Research Center, Faculty of Pharmacy, System Biology and Poisonings Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran.

A magnetic nanocarrier was synthesized in which FeO nanoparticles were encapsulated into double layers of polysaccharide shells. The first shell, which was composed of cross-linked salep polysaccharide, contained multiple nitrogen atoms in its structure and provided numerous sites for multiple functionalization. A fluorescence dye and doxorubicin, as widely used chemotherapy agent, were easily attached to the first shell and then a second shell of PEGylated carboxymethyl cellulose enveloped the drug loaded carrier to enhance its biocompatibility and regulates the drug release behavior. Read More

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http://dx.doi.org/10.1016/j.ijbiomac.2020.05.040DOI Listing

Assessing and improving the biocompatibility of microfluidic artificial lungs.

Acta Biomater 2020 May 17. Epub 2020 May 17.

VA Ann Arbor Healthcare System, 2215 Fuller Road, Ann Arbor, MI, USA, 48105; University of Michigan, 1150 W. Medical Center Drive, Ann Arbor, MI, USA, 48109.

Microfluidic artificial lungs (µALs) have the potential to improve the treatment and quality of life for patients with acute or chronic lung injury. In order to realize the full potential of this technology (including as a destination therapy), the biocompatibility of these devices needs to be improved to produce long-lasting devices that are safe for patient use with minimal or no systemic anticoagulation. Many studies exist which probe coagulation and thrombosis on polydimethyl siloxane (PDMS) surfaces, and many strategies have been explored to improve surface biocompatibility. Read More

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http://dx.doi.org/10.1016/j.actbio.2020.05.008DOI Listing

Thromboelastography as a Tool for Monitoring Blood Coagulation Dysfunction after Adequate Fluid Resuscitation can Predict Poor Outcomes in Patients with Septic Shock.

J Chin Med Assoc 2020 May 15. Epub 2020 May 15.

Department of Emergency Medicine, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, China.

Background: Coagulation abnormalities are universal in patients with septic shock and likely play a key role in multiple organ dysfunction syndrome. Early diagnosis and management of sepsis-induced coagulopathy can influence the outcome. Thromboelastography (TEG) can effectively distinguish hypercoagulability and hypocoagulability in patients with septic shock. Read More

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http://dx.doi.org/10.1097/JCMA.0000000000000345DOI Listing

Patterns of C1-Inhibitor/Plasma Serine Protease Complexes in Healthy Humans and in Hereditary Angioedema Patients.

Front Immunol 2020 5;11:794. Epub 2020 May 5.

Research Laboratory, 3rd Department of Internal Medicine, Semmelweis University, Budapest, Hungary.

C1-inhibitor (C1-INH) is an important regulator of the complement, coagulation, fibrinolytic and contact systems. The quantity of protease/C1-INH complexes in the blood is proportional to the level of the activation of these four cascade-like plasma enzyme systems. Parallel determination of C1-INH-containing activation complexes could be important to understand the regulatory role of C1-INH in diseases such as hereditary angioedema (HAE) due to C1-INH deficiency (C1-INH-HAE). Read More

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http://dx.doi.org/10.3389/fimmu.2020.00794DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7214733PMC

LPS-induced expression and release of monocyte tissue factor in patients with haemophilia.

Ann Hematol 2020 May 19. Epub 2020 May 19.

Department of Haematology and Oncology, University Cancer Centre Hamburg (UCCH), University Medical Centre Eppendorf, Hamburg, Germany.

In haemophilia, thrombin generation and fibrin deposition upon vascular injury critically depend on the tissue factor (TF)-driven coagulation pathway. TF expression by monocytes/macrophages and circulating microvesicles contributes to haemostasis, thrombosis and inflammation. Inflammation is a hallmark of blood-induced joint disease. Read More

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http://dx.doi.org/10.1007/s00277-020-04075-6DOI Listing

The thrombotic risk of spaceflight: has a serious problem been overlooked for more than half of a century?

Eur Heart J 2020 May 18. Epub 2020 May 18.

German Aerospace Center (DLR), Institute of Aerospace Medicine, Cologne, Germany.

The first ever venous thrombotic condition associated with spaceflight, an internal jugular vein thrombus requiring anticoagulation, has recently been reported. Systematic investigation of space travel-associated thrombotic risk has not been conducted. Cellular, animal, and human studies performed in ground-based models and in actual weightlessness revealed influences of weightlessness and gravity on the blood coagulation system. Read More

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http://dx.doi.org/10.1093/eurheartj/ehaa359DOI Listing

Alcohol and risk of non-traumatic bleeding events requiring hospital care in the general population: A prospective cohort study.

Alcohol 2020 May 16. Epub 2020 May 16.

National Institute of Public Health, University of Southern Denmark, Studiestræde 6, 1455 Copenhagen K, Denmark. Electronic address:

Alcohol has a direct effect on blood coagulation and fibrinolysis. We studied how alcohol is related to common (e.g. Read More

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http://dx.doi.org/10.1016/j.alcohol.2020.04.009DOI Listing

Evaluation of the Antihemostatic and Antithrombotic Effects of Lowering Coagulation Factor VII Levels in a Non-human Primate.

Cell Mol Bioeng 2020 Jun 23;13(3):179-187. Epub 2020 Mar 23.

1Department of Biomedical Engineering, Oregon Health & Science University, Portland, OR USA.

Introduction: Tissue factor (TF) and factor (F) VII, components of the extrinsic pathway of blood coagulation, are essential for hemostatic plug formation in response to injury; less clear are their roles in propagating thrombosis, as observational data in humans with congenital FVII deficiency suggests persistent thrombotic and bleeding risk even at significantly decreased FVII levels. We aimed to define the contribution of FVII to thrombus formation and hemostasis using a non-human primate model.

Methods: We treated baboons with a FVII antisense oligonucleotide (ASO) and measured platelet and fibrin deposition inside and distal to collagen- or TF-coated vascular grafts. Read More

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http://dx.doi.org/10.1007/s12195-020-00613-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7225220PMC

Inherited Bleeding Disorders in North Indian Children: 14 years' Experience from a Tertiary Care Center.

Indian J Hematol Blood Transfus 2020 Apr 21;36(2):330-336. Epub 2019 Nov 21.

1Division of Pediatric Hemato-Oncology, Department of Pediatrics, Post Graduate Institute of Medical Education and Research (PGIMER), Chandigarh, 160012 India.

Inherited bleeding disorders are not uncommon in pediatric practice: most of them being chronic, require lifelong replacement therapy. To frame a management policy, it is essential to assess the load and pattern of bleeding disorders in the local population. However, there is paucity of data reporting the clinical spectrum of coagulation and platelet function disorders in Indian children. Read More

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http://dx.doi.org/10.1007/s12288-019-01233-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7229128PMC
April 2020
0.234 Impact Factor

COVID-19 and coagulative axis: review of emerging aspects in a novel disease.

Monaldi Arch Chest Dis 2020 May 19;90(2). Epub 2020 May 19.

Department of Medicine and Health Sciences "V. Tiberio", University of Molise, Campobasso.

Latest evidences from literature suggest that SARS-CoV-2 disease 2019 (COVID-19) is commonly complicated with coagulopathy and that disseminated intravascular coagulation is present in the majority of deceased patients. Particularly, conventional coagulation parameters appear to be significantly altered in patients with poor prognosis. A wide-ranging cross- talk between coagulative haemostasis and inflammation, as well as the activation of coagulation cascade during viral infections, are well established. Read More

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http://dx.doi.org/10.4081/monaldi.2020.1300DOI Listing

Thr90Ser Mutation in Antithrombin is Associated with Recurrent Thrombosis in a Heterozygous Carrier.

Thromb Haemost 2020 May 18. Epub 2020 May 18.

Cardiovascular Biology Research Program, Oklahoma Medical Research Foundation, Oklahoma City, Oklahoma, United States.

Antithrombin (AT) is a serine protease inhibitor that regulates the activity of coagulation proteases of both intrinsic and extrinsic pathways. We identified an AT-deficient patient with a heterozygous Thr90Ser (T90S) mutation who experiences recurrent venous thrombosis. To understand the molecular basis of the clotting defect, we expressed AT-T90S in mammalian cells, purified it to homogeneity, and characterized its properties in established kinetics, binding, and coagulation assays. Read More

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http://dx.doi.org/10.1055/s-0040-1710590DOI Listing

Anticoagulant active ingredients identification of total saponin extraction of different panax medicinal plants based on grey relational analysis combined with UPLC-MS and molecular docking.

J Ethnopharmacol 2020 May 15:112955. Epub 2020 May 15.

School of Pharmacy, Hubei University of Chinese Medicine, Wuhan, Hubei, 430065, PR China. Electronic address:

Ethnopharmacological Relevance: In traditional use, Panax medical plants (ginseng, red ginseng, notoginseng, Panax japonicus, and Panacis majoris rhizoma) have different bioactivities from each other, even under different dosages, but their chemical compositions are very similar; so the question is, what is the primary effective substance induced the different efficacy, and how to identify them from a group of chemical constituents?

Aim Of The Study: The goal of this research was to provide a strategy to determine the effective substance in Panax Chinese medicinal plants responsible for the anticoagulant response.

Materials And Methods: This research used ultra-high performance liquid chromatography coupled with mass spectrometry (UPLC-MS) to analyze the saponin chemical compositions of different concentration ethanol extraction of notoginseng and the ginseng genus medical plant 75% ethanol extraction (Panax ginseng, red ginseng, Panax japonicas, Panacis majoris rhizome),and used four coagulation factors (PT, TT, APTT, Fib) to evaluate the anticoagulant activity of the extracts. Grey correlation analysis was applied to establish the spectral effect relationship and give the anticoagulant potency of different saponins. Read More

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http://dx.doi.org/10.1016/j.jep.2020.112955DOI Listing
May 2020
2.998 Impact Factor

Feasibility of an automated coagulation factor XIIIa test using its isopeptidase activity.

Anal Biochem 2020 May 15:113757. Epub 2020 May 15.

Technoclone Herstellung von Diagnostika und Arzneimitteln GmbH, Brunner Str. 67, 1230, Vienna, Austria. Electronic address:

Plasma transglutaminase FXIII provides mechanical and biochemical stability to blood clots. Congenital or acquired deficiency may be associated with bleeding diathesis and requires therefore careful monitoring. The precise automated measurement of a large number of plasma samples can provide new insights regarding the clinical relevance of certain FXIII levels. Read More

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http://dx.doi.org/10.1016/j.ab.2020.113757DOI Listing

More on "Association between ABO blood groups and risk of SARS-CoV-2 Pneumonia".

Br J Haematol 2020 May 18. Epub 2020 May 18.

Irish Centre for Vascular Biology, School of Pharmacy and Biomolecular Sciences, Royal College of Surgeons in Ireland, Dublin, Ireland.

We read with interest the recent report from Li and colleagues describing an association between ABO blood groups and risk of SARS-CoV-2 pneumonia [1]. In an initial study of 265 patients with COVID, the authors observed that blood group O individuals were significantly under-represented amongst patients who required hospitalization for severe COVID-19 infection (p < 0.01). Read More

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http://dx.doi.org/10.1111/bjh.16845DOI Listing

PECAM-1 Protects against DIC by Dampening Inflammatory Responses via Inhibiting Macrophage Pyroptosis and Restoring Vascular Barrier Integrity.

Transl Res 2020 May 14. Epub 2020 May 14.

Institute of Hematology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China; Collaborative Innovation Center of Hematology, Huazhong University of Science and Technology, Wuhan, Hubei 430022, PR China. Electronic address:

Disseminated intravascular coagulation (DIC) is a frequent complication of sepsis that affects patient outcomes due to accompanying thrombo-inflammation and microvascular permeability changes. Platelet endothelial cell adhesion molecule-1 (PECAM-1), a cellular adhesion and signaling receptor that is expressed on both hematopoietic and endothelial cells, plays an important anti-inflammatory role in acute and chronic inflammatory disease models. Little is known, however, about role and mechanism of PECAM-1 in septic DIC. Read More

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http://dx.doi.org/10.1016/j.trsl.2020.04.005DOI Listing

Endovascular Embolization Techniques in a Novel Swine Model of Fatal Uncontrolled Solid Organ Hemorrhage and Coagulopathy.

Ann Vasc Surg 2020 May 14. Epub 2020 May 14.

United States Army Institute of Surgical Research.

Introduction: Endovascular embolization is increasingly used in treating traumatic hemorrhage and other applications. No endovascular-capable translational large animal models exist and coagulopathy's effect on embolization techniques is unknown. We developed a coagulation-adaptable solid organ hemorrhage model in swine for investigation of embolization techniques. Read More

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http://dx.doi.org/10.1016/j.avsg.2020.05.004DOI Listing

Effect of 7.5% hypertonic saline solution on whole blood coagulation in healthy dogs using thromboelastography.

J Vet Emerg Crit Care (San Antonio) 2020 May 15. Epub 2020 May 15.

Laboratory of Veterinary Internal Medicine, College of Veterinary Medicine, Chungnam National University, Daejeon, South Korea.

Objective: To evaluate the effects of 7.5% hypertonic saline solution (HSS) on whole blood coagulation in healthy dogs and to compare electrolyte and osmolality measurements between in vivo and in vitro dilution with HSS.

Design: Experimental study. Read More

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http://dx.doi.org/10.1111/vec.12959DOI Listing

Cytotoxicity and hemostatic activity of chitosan/carrageenan composite wound healing dressing for traumatic hemorrhage.

Carbohydr Polym 2020 Jul 2;239:116106. Epub 2020 Mar 2.

Department of Fibres and Textile Processing Technology, Institute of Chemical Technology, Nathalal Parekh Marg, Matunga, Mumbai, 400 019, India. Electronic address:

Hemorrhage remains a big threat to trauma patients, especially in combat fields. Therefore, we formulated a biocompatible and biopolymer based chitosan/carrageenan composite dressing. This dressing was fabricated using freeze-drying that will serve as a promising material to promote hemostasis and tissue growth required during hemorrhage. Read More

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http://dx.doi.org/10.1016/j.carbpol.2020.116106DOI Listing

Global coagulation assays in transgender women on oral and transdermal estradiol therapy.

J Clin Endocrinol Metab 2020 May 15. Epub 2020 May 15.

Department of Medicine (Austin Health), The University of Melbourne, Heidelberg, VIC.

Context: The thrombotic effects of estradiol therapy in transgender women are unclear. Global coagulation assays (GCA) may be better measures of hemostatic function compared to standard coagulation tests.

Objective: To assess the GCA profiles of transgender women in comparison to cisgender controls and to compare how GCA differ between routes of estradiol therapy in transgender women. Read More

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http://dx.doi.org/10.1210/clinem/dgaa262DOI Listing
May 2020
6.209 Impact Factor

A Treg-Selective IL-2 Mutein Prevents the Formation of Factor VIII Inhibitors in Hemophilia Mice Treated With Factor VIII Gene Therapy.

Front Immunol 2020 28;11:638. Epub 2020 Apr 28.

Center for Immunity and Immunotherapies, Seattle Children's Research Institute, Seattle, WA, United States.

Hemophilia A is a genetic disorder that results in the deficiency of functional factor VIII protein, which plays a key role in blood coagulation. Currently, the majority of hemophilia A patients are treated with repeated infusions of factor VIII protein. Approximately 30% of severe hemophilia A patients develop neutralizing antibodies to factor VIII (known as factor VIII inhibitors) due to treatment, rendering factor VIII protein infusions ineffective. Read More

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http://dx.doi.org/10.3389/fimmu.2020.00638DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7198749PMC