76,518 results match your criteria Blood Cells Molecules & Diseases [Journal]


Cloning, expression profile of the complement component C9 gene and influence of the recombinant C9 protein on peripheral mononuclear leukocytes transcriptome in half-smooth tongue sole (Cynoglossus semilaevis).

Fish Shellfish Immunol 2020 May 25. Epub 2020 May 25.

College of Life Science, Qingdao University, Qingdao, 266071, China; Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266237, China. Electronic address:

The ninth complement component (C9) is a terminal complement component (TCC) that is involved in creating the membrane attack complex (MAC) on the target cell surface. In this study, the CsC9 (C9 of Cynoglossus semilaevis) cDNA sequence was cloned and characterized. The full-length CsC9 cDNA measured 2,150 bp, containing an open reading frame (ORF) of 1,803 bp, a 5'-untranslated region (UTR) of 24 bp and a 3'-UTR of 323 bp. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.fsi.2020.05.042DOI Listing

CD36-fibrin interaction propagates FXI-dependent thrombin generation of human platelets.

FASEB J 2020 May 28. Epub 2020 May 28.

Center for Thrombosis and Hemostasis (CTH), University Medical Center, Johannes Gutenberg-University Mainz, Mainz, Germany.

Thrombin converts fibrinogen to fibrin and activates blood and vascular cells in thrombo-inflammatory diseases. Platelets are amplifiers of thrombin formation when activated by leukocyte- and vascular cell-derived thrombin. CD36 on platelets acts as sensitizer for molecules with damage-associated molecular patterns, thereby increasing platelet reactivity. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1096/fj.201903189RDOI Listing

Systematic Investigation of Polyurethane Biomaterial Surface Roughness on Human Immune Responses .

Biomed Res Int 2020 11;2020:3481549. Epub 2020 May 11.

NMI, Natural and Medical Sciences Institute at the University of Tübingen, Markwiesenstr. 55, 72770 Reutlingen, Germany.

It has been widely shown that biomaterial surface topography can modulate host immune response, but a fundamental understanding of how different topographies contribute to pro-inflammatory or anti-inflammatory responses is still lacking. To investigate the impact of surface topography on immune response, we undertook a systematic approach by analyzing immune response to eight grades of medical grade polyurethane of increasing surface roughness in three models of the human immune system. Polyurethane specimens were produced with defined roughness values by injection molding according to the VDI 3400 industrial standard. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1155/2020/3481549DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7240656PMC

Contribution of the SYK Tyrosine kinase expression to human iNKT self-reactivity.

Eur J Immunol 2020 May 27. Epub 2020 May 27.

Université de Nantes, CNRS, Inserm, CRCINA, Nantes, F-44000, France.

Invariant Natural Killer T (iNKT) cells are particular T lymphocytes, at the frontier between innate and adaptative immunities. They participate in the elimination of pathogens or tumor cells, but also in the development of allergic reactions and autoimmune diseases. From their first descriptions, the phenomenon of self-reactivity has been described. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/eji.201948416DOI Listing

Hydroxylated Rotenoids Selectively Inhibit the Proliferation of Prostate Cancer Cells.

J Nat Prod 2020 May 27. Epub 2020 May 27.

Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge, CB2 1EW, U.K.

Prostate cancer is one of the leading causes of cancer-related death in men. The identification of new therapeutics to selectively target prostate cancer cells is therefore vital. Recently, the rotenoids rotenone () and deguelin () were reported to selectively kill prostate cancer cells, and the inhibition of mitochondrial complex I was established as essential to their mechanism of action. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.jnatprod.9b01224DOI Listing

Evolutionary links between intra- and extracellular acid-base regulation in fish and other aquatic animals.

J Exp Zool A Ecol Integr Physiol 2020 May 26. Epub 2020 May 26.

Department of Zoology, University of British Columbia, Vancouver, Canada.

The acid-base relevant molecules carbon dioxide (CO ), protons (H ), and bicarbonate (HCO ) are substrates and end products of some of the most essential physiological functions including aerobic and anaerobic respiration, ATP hydrolysis, photosynthesis, and calcification. The structure and function of many enzymes and other macromolecules are highly sensitive to changes in pH, and thus maintaining acid-base homeostasis in the face of metabolic and environmental disturbances is essential for proper cellular function. On the other hand, CO , H , and HCO have regulatory effects on various proteins and processes, both directly through allosteric modulation and indirectly through signal transduction pathways. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/jez.2367DOI Listing

How Does Ginsenoside Rh2 Mitigate Adipogenesis in Cultured Cells and Obese Mice?

Molecules 2020 May 21;25(10). Epub 2020 May 21.

Department of Human Sciences, Tennessee State University, Nashville, TN 37209, USA.

Ginsenoside Rh2, an intermediate metabolite of ginseng, but not naturally occurring, has recently drawn attention because of its anticancer effect. However, it is not clear if and how Rh2 inhibits preadipocytes differentiation. In the present study, we hypothesized that ginsenoside Rh2 attenuates adipogenesis through regulating the peroxisome proliferator-activated receptor gamma (PPAR-γ) pathway both in cells and obese mice. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3390/molecules25102412DOI Listing

T cell Co-Stimulatory molecules ICOS and CD28 stratify idiopathic pulmonary fibrosis survival.

Respir Med X 2019 1;1. Epub 2019 Feb 1.

Pulmonary and Critical Care Medicine, Department of Medicine, University of Chicago, Chicago, IL, USA.

Idiopathic pulmonary fibrosis (IPF) is a devastating disease that kills as many Americans as breast cancer each year. This study investigated whether lung function decline and survival associates with adaptive immunity in patients with IPF, specifically the expression of checkpoint molecules ICOS, CD28 and PD-1 on circulating CD4 T cells. Clinical data, blood samples and pulmonary function tests were collected prospectively and longitudinally from 59 patients with IPF over a study period of 5 years. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.yrmex.2019.100002DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7243672PMC
February 2019

Effect of peripheral blood-derived mesenchymal stem cells on macrophage polarization and Th17/Treg balance .

Regen Ther 2020 Jun 16;14:275-283. Epub 2020 May 16.

Key Laboratory of Cell Engineering of Guizhou Province and Regenerative Medicine Centre, Affiliated Hospital of Zunyi Medical University, Zunyi, Guizhou, China.

Introduction: Mesenchymal stem cells (MSCs) have always been the center of the experimental exploration of regenerative therapy together with other stem cells. Among with, peripheral blood-derived mesenchymal stem cells (PBMSCs) have been regarded as promising in clinical applications for its convenience of acquisition from peripheral blood. However, few reported experiments so far to elucidate the exact mechanisms of how PBMSC influence regeneration. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.reth.2020.03.008DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7232039PMC

Evaluation of blood-testis barrier integrity in terms of adhesion molecules in nonobstructive azoospermia.

Andrologia 2020 May 26:e13636. Epub 2020 May 26.

Department of Histology and Embryology, Faculty of Medicine, Ankara University, Ankara, Turkey.

Blood-testis barrier (BTB) is critical for maintaining fertility. The integrity of tight junctions (TJs) provides restricted permeability of BTB. The aim of this study was to evaluate the relationship between BTB and Sertoli cells. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/and.13636DOI Listing

Myeloma cells induce the accumulation of activated CD94low NK cells by cell-to-cell contacts involving CD56 molecules.

Blood Adv 2020 May;4(10):2297-2307

Laboratory of Immunology and Biotherapy and.

Natural killer (NK) cells represent innate effector cells potentially able to play a role during the immune response against multiple myeloma (MM). To better define the distribution and the specific properties of NK cell subsets during MM disease, we analyzed their features in the bone marrow and peripheral blood of newly diagnosed MM patients. Our findings revealed that, in both compartments, NK cells were more abundant than in healthy donors. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1182/bloodadvances.2019000953DOI Listing

Artificial feeder cells expressing ligands for killer cell immunoglobulin-like receptors and CD94/NKG2A for expansion of functional primary natural killer cells with tolerance to self.

Cytotherapy 2020 May 22. Epub 2020 May 22.

Department of Neurosurgery, Section Experimental Neurosurgery/Tumor Immunology, University Hospital Carl Gustav Carus, Technische Universitat Dresden, Dresden, Germany; German Cancer Consortium (DKTK), Partner Site Dresden, Dresden, Germany; German Cancer Research Center (DKFZ), Heidelberg, Germany; National Center for Tumor Diseases (NCT), Dresden, Germany. Electronic address:

Background Aims: Natural killer (NK) cells are promising cells for immunotherapy of cancer, and there are ongoing efforts to improve their ex vivo expansion to clinically relevant numbers. This study focused on the development of a C1-, C2-, Bw4 killer cell immunoglobulin-like receptor (KIR) ligand and NKG2A ligand-containing feeder cell line for autonomous expansion of functional NK cells.

Methods: PC3-derived feeder cells expressing IL-2, 4-1BBL and membrane-bound IL-15-mutDAP12 (mIL-15d) fusion protein in combinations or alone were generated and used for expansion. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jcyt.2020.02.004DOI Listing

Ability of the immune system to fight viruses highlighted by cytometry and TCR clonotype assessments: lessons taken prior to COVID-19 virus pandemic outbreak.

Pol Arch Intern Med 2020 May 25. Epub 2020 May 25.

The intriguing aspects of SARS-CoV-2 virus are the high rate of spread and rapid progression of pneumonitis. Confronted with thousands of deaths daily worldwide, we have to build quickly the rationale behind the treatment, taking advantage from past analogues. When a new virus strikes, T-cell receptor (TCR) gamma/delta cells are in the first line of defence, activated by stress molecules and recognising some epitopes in a process that is major histocompatibility complex (MHC) independent but still specific, e. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.20452/pamw.15388DOI Listing

[CD19 antigen loss after treatment of Bispecific T-cell Engager and effective response to salvage bispecific CAR-T therapy in B cell acute lymphoblastic leukemia: a case report and literature review].

Zhonghua Xue Ye Xue Za Zhi 2020 Apr;41(4):282-286

State Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Diseases, Institute of Hematology & Blood Diseases Hospital, CAMS & PUMC, Tianjin 300020, China.

To analyze the influence of CD19 isoforms to the efficacy of CD19/CD3 Bispecific T-cell Engager (BiTE) antibody, and explore the resistance mechanism of BiTE immunotherapy. Semi-quantitative RT-PCR (qRT-PCR) was used to detect the expression of CD19 mRNA isoforms before and after BiTE treatment in a patient with CD19(+) B cell acute lymphoblastic leukemia (ALL) . CD19 isoforms were analyzed by Sanger sequencing. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3760/cma.j.issn.0253-2727.2020.04.004DOI Listing

Proteomics and transcriptomics study reveals the utility of ISGs as novel molecules for early pregnancy diagnosis in dairy cows.

J Reprod Immunol 2020 May 19;140:103148. Epub 2020 May 19.

Lactation and Immuno-Physiology Laboratory, ICAR-National Dairy Research Institute, Karnal, Haryana 132001, India. Electronic address:

An early and precise diagnosis of pregnancy in cows is critical to short the calving interval and to improve their reproductive efficiency. Neutrophils are the first blood cells to sensitize the embryo in the uterus and participate in maternal recognition of pregnancy after getting induced by interferon tau (IFNτ). To study the protein abundance ratio, blood samples were collected on 0th, 10th, 18th and 36th day post-artificial insemination (AI) from crossbred Karan Fries cows. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jri.2020.103148DOI Listing

Mast cells and complement system: Ancient interactions between components of innate immunity.

Allergy 2020 May 23. Epub 2020 May 23.

Department of Bacteriology and Immunology, Immunobiology Research Program, University of Helsinki, Helsinki, Finland.

The emergence and evolution of the complement system and mast cells (MCs) can be traced back to sea urchins and the ascidian Styela plicata, respectively. Acting as a cascade of enzymatic reactions, complement is activated through the classical (CP), the alternative (AP) and the lectin pathway (LP) based on the recognized molecules. The system's main biological functions include lysis, opsonization and recruitment of phagocytes. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/all.14413DOI Listing

Discovery of novel N-substituted thiazolidinediones (TZDs) as HDAC8 inhibitors: in-silico studies, synthesis, and biological evaluation.

Bioorg Chem 2020 May 15;100:103934. Epub 2020 May 15.

Department of Pharmaceutical Chemistry, Bharati Vidyapeeth's College of Pharmacy, Navi Mumbai, India. Electronic address:

Epigenetics plays a fundamental role in cancer progression, and developing agents that regulate epigenetics is crucial for cancer management. Among Class I and Class II HDACs, HDAC8 is one of the essential epigenetic players in cancer progression. Therefore, we designed, synthesized, purified, and structurally characterized novel compounds containing N-substituted TZD (P1-P25). Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bioorg.2020.103934DOI Listing

Surmounting the endothelial barrier for delivery of drugs and imaging tracers.

Atherosclerosis 2020 May 11. Epub 2020 May 11.

Amsterdam UMC, University of Amsterdam, Department of Experimental Vascular Medicine, Amsterdam Cardiovascular Sciences, Meibergdreef 9, Amsterdam, Netherlands. Electronic address:

The endothelium is crucial for maintaining vascular homeostasis and functions as a barrier between blood components and tissue. In atherosclerosis, this barrier function is impaired, which is characterized by secretion of chemoattractants and cytokines, upregulation of adhesion molecules and increased vascular permeability. This facilitates enhanced leukocyte migration through the vessel wall. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.atherosclerosis.2020.04.025DOI Listing

Engagement of people with multiple sclerosis to enhance research into the physiological effect of hyperbaric oxygen therapy.

Mult Scler Relat Disord 2020 Apr 29;43:102084. Epub 2020 Apr 29.

Institute of Biomedical and Clinical Sciences, College of Medicine & Healthcare, University of Exeter, Exeter, UK; Royal Devon and Exeter Hospital Foundation Trust, Exeter, UK.

Background: Thousands of people with multiple sclerosis (MS) have used self-administered oxygen therapy in the UK. Clinical trials have been performed, with scant evidence that people with MS have been consulted to explore how they benefit from or how to optimize this treatment. The conventional MS disease disability scores used in trials seldom reflect the effects individuals report when using oxygen therapy to treat their symptoms. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.msard.2020.102084DOI Listing

Enhanced osteogenesis of hydroxyapatite scaffolds by coating with BMP-2-loaded short polylactide nanofiber: a new drug loading method for porous scaffolds.

Regen Biomater 2020 Feb 7;7(1):91-98. Epub 2019 Nov 7.

Sichuan Provincial Laboratory of Orthopaedic Engineering, Department of Bone and Joint Surgery, Affiliated Hospital of Southwest Medical University, Luzhou 646000, China.

Porous hydroxyapatite (HA) is widely used in porous forms to assist bone defect healing. However, further improvements in biological functions are desired for meeting complex clinical situations such as impaired bone regeneration in poor bone stock. The extracellular matrix (ECM) of human tissues is characterized by nanofibrous structures and a variety of signal molecules. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1093/rb/rbz040DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7233607PMC
February 2020

The NLRP3-Mediated Neuroinflammatory Responses to CdTe Quantum Dots and the Protection of ZnS Shell.

Int J Nanomedicine 2020 6;15:3217-3233. Epub 2020 May 6.

Key Laboratory of Environmental Medicine and Engineering, Ministry of Education, School of Public Health, Southeast University, Nanjing 210009, People's Republic of China.

Introduction: Since CdTe quantum dots (QDs) are still widely considered as advanced fluorescent probes because of their far superior optical performance and fluorescence efficiency over non-cadmium QDs, it is important to find ways to control their toxicity.

Methods: In this study, the adverse effects of two cadmium-containing QDs, ie, CdTe QDs and CdTe@ZnS QDs, on the nervous system of nematode , the hippocampus of mice, and cultured microglia were measured in order to evaluate the neuroinflammation caused by cadmium-containing QDs and the potential mechanisms.

Results: Firstly, we observed that cadmium-containing QD exposure-induced immune responses and neurobehavioral deficit in nematode . Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.2147/IJN.S246578DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7212783PMC

Thermodynamic interference with bile acid demicelleization reduces systemic entry and injury during cholestasis.

Sci Rep 2020 May 21;10(1):8462. Epub 2020 May 21.

Department of Medicine, Mayo Clinic, Scottsdale, AZ, USA.

Bile acids (BA), with their large hydrophobic steroid nucleus and polar groups are amphipathic molecules. In bile, these exist as micelles above their critical micellar concentration (CMC). In blood at low concentrations, these exist as monomers, initiating cellular signals. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41598-020-65451-wDOI Listing

Inhibition of circulating exosomal microRNA-15a-3p accelerates diabetic wound repair.

Aging (Albany NY) 2020 May 21;12. Epub 2020 May 21.

Department of Orthopedics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.

Diabetic foot ulcers are a common complication of diabetes, and are usually incurable in the clinic. Exosomes (carriers that transfer endogenous molecules) from diabetic patients' blood have been demonstrated to suppress diabetic wound repair. In this study, we investigated the effects of circulating exosomal microRNA-15a-3p (miR-15a-3p) on diabetic wound repair. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.18632/aging.103143DOI Listing

PD-L1 targeting high-affinity NK (t-haNK) cells induce direct antitumor effects and target suppressive MDSC populations.

J Immunother Cancer 2020 May;8(1)

Laboratory of Tumor Immunology and Biology, Center for Cancer Research, National Cancer Institute, Bethesda, Maryland, USA

Background: Although immune checkpoint inhibitors have revolutionized cancer treatment, clinical benefit with this class of agents has been limited to a subset of patients. Hence, more effective means to target tumor cells that express immune checkpoint molecules should be developed. For the first time, we report a novel natural killer (NK) cell line, programmed death-ligand 1 (PD-L1) targeting high-affinity natural killer (t-haNK), which was derived from NK-92 and was engineered to express high-affinity CD16, endoplasmic reticulum-retained interleukin (IL)-2, and a PD-L1-specific chimeric antigen receptor (CAR). Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1136/jitc-2019-000450DOI Listing

PECAM-1 overexpression signifies aggressive biologic behavior of oral lichen planus - A pilot study.

Indian J Dent Res 2020 Mar-Apr;31(2):277-281

Department of Oral Pathology and Microbiology, Faculty of Dental Sciences, M S Ramaiah University of Applied Sciences, M S R Nagar, Bangalore, Karnataka, India.

Context: The etiopathogenesis of oral lichen planus (OLP) is still debatable. According to literature, many studies have illustrated OLP as a T-cell-mediated chronic autoimmune disease. Currently, there is increased evidence of chronic inflammation in OLP and its association with vascular adhesion molecules (VAMs). Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.4103/ijdr.IJDR_653_18DOI Listing

Differential Expression of IgM and IgD Discriminates Two Subpopulations of Human Circulating IgMIgDCD27 B Cells That Differ Phenotypically, Functionally, and Genetically.

Front Immunol 2020 6;11:736. Epub 2020 May 6.

Instituto de Genética Humana, Facultad de Medicina, Pontificia Universidad Javeriana, Bogotá, Colombia.

The origin and function of blood IgMIgDCD27 B cells is controversial, and they are considered a heterogeneous population. Previous staining of circulating B cells of healthy donors with rotavirus fluorescent virus-like particles allowed us to differentiate two subsets of IgMIgDCD27: IgM and IgM B cells. Here, we confirmed this finding and compared the phenotype, transcriptome, function, and Ig gene repertoire of these two subsets. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3389/fimmu.2020.00736DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7219516PMC

Magnetic nanoparticles double wrapped into cross-linked salep/PEGylated carboxymethyl cellulose; a biocompatible nanocarrier for pH-triggered release of doxorubicin.

Int J Biol Macromol 2020 May 8;158:994-1006. Epub 2020 May 8.

Chemical Injuries Research Center, Faculty of Pharmacy, System Biology and Poisonings Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran.

A magnetic nanocarrier was synthesized in which FeO nanoparticles were encapsulated into double layers of polysaccharide shells. The first shell, which was composed of cross-linked salep polysaccharide, contained multiple nitrogen atoms in its structure and provided numerous sites for multiple functionalization. A fluorescence dye and doxorubicin, as widely used chemotherapy agent, were easily attached to the first shell and then a second shell of PEGylated carboxymethyl cellulose enveloped the drug loaded carrier to enhance its biocompatibility and regulates the drug release behavior. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ijbiomac.2020.05.040DOI Listing

Potential of Chitosan and Its Derivatives for Biomedical Applications in the Central Nervous System.

Front Bioeng Biotechnol 2020 5;8:389. Epub 2020 May 5.

Biotecnología Industrial, CONACYT Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco (CIATEJ), Zapopan, Mexico.

It is well known that the central nervous system (CNS) has a limited regenerative capacity and that many therapeutic molecules cannot cross the blood brain barrier (BBB). The use of biomaterials has emerged as an alternative to overcome these limitations. For many years, biomedical applications of chitosan have been studied due to its remarkable biological properties, biocompatibility, and high versatility. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3389/fbioe.2020.00389DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7214799PMC

Platelets in Neurodegenerative Conditions-Friend or Foe?

Front Immunol 2020 5;11:747. Epub 2020 May 5.

Queensland Brain Institute, The University of Queensland, Brisbane, QLD, Australia.

It is now apparent that platelet function is more diverse than originally thought, shifting the view of platelets from blood cells involved in hemostasis and wound healing to major contributors to numerous regulatory processes across different tissues. Given their intriguing ability to store, produce and release distinct subsets of bioactive molecules, including intercellular signaling molecules and neurotransmitters, platelets may play an important role in orchestrating healthy brain function. Conversely, a number of neurodegenerative conditions have recently been associated with platelet dysfunction, further highlighting the tissue-independent role of these cells. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3389/fimmu.2020.00747DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7214916PMC

Exploratory analysis of immune checkpoint receptor expression by circulating T cells and tumor specimens in patients receiving neo-adjuvant chemotherapy for operable breast cancer.

BMC Cancer 2020 May 19;20(1):445. Epub 2020 May 19.

The Ohio State University Comprehensive Cancer Center, The Ohio State University, 410 W 12th Avenue, Columbus, OH, 43210, USA.

Background: While combinations of immune checkpoint (ICP) inhibitors and neo-adjuvant chemotherapy (NAC) have begun testing in patients with breast cancer (BC), the effects of chemotherapy on ICP expression in circulating T cells and within the tumor microenvironment are still unclear. This information could help with the design of future clinical trials by permitting the selection of the most appropriate ICP inhibitors for incorporation into NAC.

Methods: Peripheral blood samples and/or tumor specimens before and after NAC were obtained from 24 women with operable BC. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1186/s12885-020-06949-4DOI Listing

Platelet Toll-Like Receptors Mediate Thromboinflammatory Responses in Patients With Essential Thrombocythemia.

Front Immunol 2020 30;11:705. Epub 2020 Apr 30.

Institute of Medical Research A. Lanari, School of Medicine, University of Buenos Aires, Buenos Aires, Argentina.

Essential thrombocythemia (ET) is comprised among chronic myeloproliferative neoplasms (MPN) and is caused by driver mutations in 2, , and , which lead to megakaryocyte proliferation and prominent thrombocytosis. Thrombosis remains the main cause of morbidity in ET and is driven by the interplay between blood cells, the endothelium, the clotting cascade, and host-derived inflammatory mediators. Platelet activation plays a key role in the thrombotic predisposition, although the underlying mechanisms remain poorly defined. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3389/fimmu.2020.00705DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7203216PMC

PDE3 Inhibition Reduces Epithelial Mast Cell Numbers in Allergic Airway Inflammation and Attenuates Degranulation of Basophils and Mast Cells.

Front Pharmacol 2020 1;11:470. Epub 2020 May 1.

Department of Pulmonary Medicine, Erasmus MC, Rotterdam, Netherlands.

Epithelial mast cells are generally present in the airways of patients with allergic asthma that are inadequately controlled. Airway mast cells (MCs) are critically involved in allergic airway inflammation and contribute directly to the main symptoms of allergic patients. Phosphodiesterase 3 (PDE3) tailors signaling of cyclic adenosine monophosphate (cAMP) and cyclic guanosine monophosphate (cGMP), which are critical intracellular second messenger molecules in various signaling pathways. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3389/fphar.2020.00470DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7206980PMC

Mechanistic Basis of ex Vivo Umbilical Cord Blood Stem Progenitor Cell Expansion.

Stem Cell Rev Rep 2020 May 18. Epub 2020 May 18.

Division of Hematology/Oncology, Department of Medicine, University of Illinois College of Medicine Chicago, 840 South Wood Street, Clinical Sciences Building (CSB) Rm# 826, Chicago, IL, 60612, USA.

Umbilical cord blood (CB) transplantation has been used successfully in humans for three decades due to its rapid availability for patients lacking a suitable allogeneic donor, less stringent HLA matching requirements, and low rates of relapse and chronic graft-versus-host disease (GVHD). However, CB transplantation is associated with complications, such as delayed hematopoietic engraftment, graft failure, which increases infection and bleeding and causes longer hospital stays, and transplant-related mortality. The majority of these biological limitations are due to the unforeseeable functional potency of multipotent hematopoietic stem cells (HSCs), which reduce the predictability of successful transplantation; however, several strategies have been developed to increase the number of hematopoietic stem progenitor cells (HSPCs) infused during CB transplantation. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s12015-020-09981-wDOI Listing

Preparation of Protein-Peptide-Calcium Phosphate Composites for Controlled Protein Release.

Molecules 2020 May 14;25(10). Epub 2020 May 14.

National Institute of Advanced Industrial Science and Technology (AIST), 2266-98 Anagahora, Shimoshidami, Moriyama-ku, Nagoya 463-8560, Japan.

Protein-peptide-calcium phosphate composites were developed for achieving sustainable and controlled protein release. Bovine serum albumin (BSA) as a model acidic protein was efficiently encapsulated with basic polypeptides such as polylysine and polyarginine during the precipitation of calcium phosphate (CaP). The prepared composites were fully characterized in terms of their morphologies, crystallinities, and the porosity of their structures, and from these analyses, it was observed that there are no significant differences between the composites. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3390/molecules25102312DOI Listing

Development of Novel Analogs of the Monocarboxylate Transporter Ligand FACH and Biological Validation of One Potential Radiotracer for Positron Emission Tomography (PET) Imaging.

Molecules 2020 May 14;25(10). Epub 2020 May 14.

Department of Neuroradiopharmaceuticals, Institute of Radiopharmaceutical Cancer Research, Helmholtz-Zentrum Dresden-Rossendorf, Permoserstraße 15, 04318 Leipzig, Germany.

Monocarboxylate transporters 1-4 (MCT1-4) are involved in several metabolism-related diseases, especially cancer, providing the chance to be considered as relevant targets for diagnosis and therapy. [F]FACH was recently developed and showed very promising preclinical results as a potential positron emission tomography (PET) radiotracer for imaging of MCTs. Given that [F]FACH did not show high blood-brain barrier permeability, the current work is aimed to investigate whether more lipophilic analogs of FACH could improve brain uptake for imaging of gliomas, while retaining binding to MCTs. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3390/molecules25102309DOI Listing

Two-Faced Roles of Tumor-Associated Neutrophils in Cancer Development and Progression.

Int J Mol Sci 2020 May 14;21(10). Epub 2020 May 14.

Division of Molecular Bioregulation, Cancer Research Institute, Kanazawa University, Kakuma-machi, Kanazawa, Ishikawa 920-1192, Japan.

Neutrophils are the most abundant circulating leukocytes in humans. Neutrophil infiltration into tumor tissues has long been observed but its roles have been ignored due to the presumed short life cycle and metabolic incompetence of neutrophils. Recent advances in neutrophil biology research have revealed that neutrophils have a longer life cycle with a potential to express various bioactive molecules. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3390/ijms21103457DOI Listing

Thr90Ser Mutation in Antithrombin is Associated with Recurrent Thrombosis in a Heterozygous Carrier.

Thromb Haemost 2020 May 18. Epub 2020 May 18.

Cardiovascular Biology Research Program, Oklahoma Medical Research Foundation, Oklahoma City, Oklahoma, United States.

Antithrombin (AT) is a serine protease inhibitor that regulates the activity of coagulation proteases of both intrinsic and extrinsic pathways. We identified an AT-deficient patient with a heterozygous Thr90Ser (T90S) mutation who experiences recurrent venous thrombosis. To understand the molecular basis of the clotting defect, we expressed AT-T90S in mammalian cells, purified it to homogeneity, and characterized its properties in established kinetics, binding, and coagulation assays. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1055/s-0040-1710590DOI Listing

CD70-silenced dendritic cells induce immune tolerance in immune thrombocytopenia patients.

Br J Haematol 2020 May 18. Epub 2020 May 18.

State Key Laboratory of Experimental Hematology, National Clinical Research Center for Hematological Disorders, Institute of Hematology and Blood Diseases Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, China.

The hyper-response of dendritic cell (DC) is believed to participate in the pathogenesis of immune thrombocytopenia (ITP). The CD70 expression on the surface of DCs that takes part in the CD27-CD70 costimulation pathway is an important element of DC 'licensing', which may initiate a series of autoreactive immune responses. To elucidate the roles CD70 molecules play in the DCs of ITP patients, we first stimulated the CD70 molecules on the DCs of ITP patients and normal controls, and found that the stimulated DCs from ITP patients exhibited higher ability to induce CD4 CD25 T lymphocytes proliferation, while lowering the ability of the induction of regulatory T cells (Tregs) from CD4 CD25 T lymphocytes. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/bjh.16689DOI Listing

Normal ageing of the brain: Histological and biological aspects.

Rev Neurol (Paris) 2020 May 14. Epub 2020 May 14.

Inserm, UMR-S 1172 « Alzheimer et Tauopathies », centre mémoire de ressources et de recherche, Labex DISTALZ, université de Lille, CHU de Lille, 59000 Lille, France; Pôle de neurologie, CHU de Lille, 59000 Lille, France.

All the hallmarks of ageing are observed in the brain, and its cells, especially neurons, are characterized by their remarkably long lifetime. Like any organ or system, the brain is exposed to ageing processes which affect molecules, cells, blood vessels, gross morphology and, uniquely for this organ, cognition. The preponderant cerebral structures are characterized by the cellular processes of neurons and glial cells and while the quantity of cerebral interstitial fluid is limited, it is now recognized as playing a crucial role in maintaining cerebral homeostasis. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.neurol.2020.03.017DOI Listing

Counter-regulatory 'Renin-Angiotensin' System-based Candidate Drugs to Treat COVID-19 Diseases in SARS-CoV-2-infected patients.

Infect Disord Drug Targets 2020 May 17. Epub 2020 May 17.

Université Aix-Marseille, Institut de Neuro-physiopathologie (INP), UMR 7051, Faculté de Pharmacie, 27 Bd Jean Moulin, 13385 Marseille Cedex. France.

SARS-CoV-2 / COVID-19 and the 'Renin-Angiotensin' System The ubiquitous 'Renin-Angiotensin' system (RAS), also referred to as 'Renin-Angiotensin-Aldosterone' system, plays a crucial physiological role in humans as being a key regulator of renal, cardiovascular and innate immune functions [1, 2]. It appears to work in tandem with vitamin D, a secosteroid pro-hormone which reportedly acts as a negative regulatory factor of the RAS [3-6]. A dysfunction (e. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.2174/1871526520666200518073329DOI Listing

Glucocorticoids and the cytokines IL-12, IL-15 and IL-18 present in the tumor microenvironment induce PD-1 expression on human Natural Killer cells.

J Allergy Clin Immunol 2020 May 14. Epub 2020 May 14.

Department of Immunology, IRCCS Bambino Gesù Children's Hospital, Rome, Italy. Electronic address:

Background: PD-1 immune checkpoint blockade has provided significant clinical efficacy across various types of cancer by unleashing both T- and NK cell-mediated anti-tumor responses. However, resistance to immunotherapy occurs for many patients, rendering the identification of the mechanisms that control PD-1 expression extremely important to increase the response to the therapy.

Objective: To identify the stimuli and the molecular mechanisms that induce the de novo PD-1 expression on human NK cells in the tumor setting. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jaci.2020.04.044DOI Listing

Lymphopenia and lymphocytosis in practical healthy people born and living in the North.

Immun Inflamm Dis 2020 May 16. Epub 2020 May 16.

N. Laverov Federal Center for Integrated Arctic Research, Russian Academy of Sciences, Institute of Environmental Physiology, Department of Environmental Immunology, Arkhangelsk, Russian Federation.

Introduction: The purpose of this study was to elucidate the mechanisms of the formation of lymphopenia and lymphocytosis in healthy people, who are living and working in the Arctic region.

Materials And Methods: A total of 88 practically healthy people living and working in the Arctic region were examined. An analysis of the results was carried out, depending on the concentration of lymphocytes in the peripheral venous blood: group 1-with lymphopenia, the content of lymphocytes below 1. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/iid3.308DOI Listing

Characterization of Plasma-Derived Small Extracellular Vesicles Indicates Ongoing Endothelial and Platelet Activation in Patients with Thrombotic Antiphospholipid Syndrome.

Cells 2020 May 13;9(5). Epub 2020 May 13.

Department of Rheumatology, University Medical Centre Ljubljana, SI-1000 Ljubljana, Slovenia.

Antiphospholipid syndrome (APS) is a systemic autoimmune disease, characterized by thrombosis, obstetric complications and the presence of antiphospholipid antibodies (aPL), which drive endothelial injury and thrombophilia. Extracellular vesicles (EVs) have been implicated in endothelial and thrombotic pathologies. Here, we characterized the quantity, cellular origin and the surface expression of biologically active molecules in small EVs (sEVs) isolated from the plasma of thrombotic APS patients ( = 14), aPL-negative patients with idiopathic thrombosis (aPL-neg IT, = 5) and healthy blood donors (HBD, = 7). Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3390/cells9051211DOI Listing

A human H1-HBB11-GFP reporter embryonic stem cell line (WAe001-A-2) generated using TALEN-based genome editing.

Stem Cell Res 2020 May 6;45:101837. Epub 2020 May 6.

Black Family Stem Cell Institute, Icahn School of Medicine at Mount Sinai, New York, NY, USA; Department of Cellular, Developmental, and Regenerative Biology, Icahn School of Medicine at Mount Sinai, New York, NY, USA. Electronic address:

Hemoglobin production during mammalian development is characterized by temporal switches of the genes coding for the α- and ß-globin chains. Defects in this controlled process can lead to hemoglobinapathies such as sickle cell disease and ß-thalassemia. The ability of human embryonic stem cells (hESC) to proceed through hematopoiesis could provide a clinically useful source of red blood cells. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.scr.2020.101837DOI Listing

Ultrasmall gold nanoparticles (2 nm) can penetrate and enter cell nuclei in an in vitro 3D brain spheroid model.

Acta Biomater 2020 May 12. Epub 2020 May 12.

Inorganic Chemistry and Center for Nanointegration Duisburg-Essen (CeNIDE), University of Duisburg-Essen, Essen, 45117, Germany. Electronic address:

The neurovascular unit (NVU) is a complex functional and anatomical structure composed of endothelial cells and their blood-brain barrier (BBB) forming tight junctions. It represents an efficient barrier for molecules and drugs. However, it also prevents a targeted transport for the treatment of cerebral diseases. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.actbio.2020.04.023DOI Listing

Phagocyte function of peripheral neutrophil granulocytes and monocytes in endometriosis before and after surgery.

J Gynecol Obstet Hum Reprod 2020 May 12:101796. Epub 2020 May 12.

Department of Obstetrics and Gynecology, Univesity of Debrecen, Faculty of Medicine, 98. Nagyerdei krt., Debrecen 4012, Hungary. Electronic address:

Introduction: Endometriosis is a chronic systemic disease, which influence negatively the quality of life of affected women and responsible for infertility and chronic pelvic pain. Pathophysiology of the disease is still enigmatic, but insufficient immune surveillance may play a role in it. Peripheral natural immune cell function is rarely examined. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jogoh.2020.101796DOI Listing

Tithonia diversifolia-derived orizabin suppresses cell adhesion, differentiation, and oxidized LDL accumulation by Akt signaling suppression via PTEN promotion in THP-1 cells.

J Food Biochem 2020 May 15:e13268. Epub 2020 May 15.

Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayama, Japan.

As a Japanese folk medicine, Tithonia diversifolia is used for cardiovascular disease prevention and health maintenance. We isolated T. diversifolia-derived orizabin based on the nitric oxide production inhibitory effect. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/jfbc.13268DOI Listing

Sickle Cell Anemia Patients Display an Intricate Cellular and Serum Biomarker Network Highlighted by TCD4+CD69+ Lymphocytes, IL-17/MIP-1, IL-12/VEGF, and IL-10/IP-10 Axis.

J Immunol Res 2020 8;2020:4585704. Epub 2020 Jan 8.

Programa de Pós-Graduação em Imunologia Básica e Aplicada, Universidade Federal do Amazonas (UFAM), 69077-000 Manaus, AM, Brazil.

Background: Sickle cell anemia (SCA) is associated with a chronic proinflammatory state characterized by elevated leukocyte count, mortality from severe recurrent infections, and subsequent vasoocclusive complications with leukocyte adhesion to the endothelium and increased plasma levels of inflammatory cytokines. The immune system has a close connection with morbidity in SCA, but further studies are needed to uncover the involvement of innate and adaptive immunities in modulating the SCA physiopathology. We performed measurements of the frequency of innate and adaptive immunity cells, cytokines, chemokines, and growth factors and immunophenotyping of Toll-like receptor and adhesion molecule expression in the blood of SCA patients and healthy donors to evaluate the different profiles of these biomarkers, the relationship among them, and their correlation to laboratory records and death risk. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1155/2020/4585704DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7199620PMC
January 2020

Neuron tau-targeting biomimetic nanoparticles for curcumin delivery to delay progression of Alzheimer's disease.

J Nanobiotechnology 2020 May 13;18(1):71. Epub 2020 May 13.

State Key Laboratory of Toxicology and Medical Countermeasures, Beijing Institute of Pharmacology and Toxicology, Beijing, 100850, China.

Background: Although many therapeutic strategies for Alzheimer's disease (AD) have been explored, these strategies are seldom used in the clinic. Therefore, AD therapeutic research is still urgently needed. One major challenge in the field of nanotherapeutics is to increase the selective delivery of drugs to a targeted location. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1186/s12951-020-00626-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7222444PMC

Therapeutic potential of lipids obtained from γ-irradiated PBMCs in dendritic cell-mediated skin inflammation.

EBioMedicine 2020 May 8;55:102774. Epub 2020 May 8.

Research Division of Biology and Pathobiology of the Skin, Department of Dermatology, Medical University of Vienna, Lazarettgasse 14, 1090 Vienna, Austria. Electronic address:

Background: Since numerous pathological conditions are evoked by unwanted dendritic cell (DC) activity, therapeutic agents modulating DC functions are of great medical interest. In regenerative medicine, cellular secretomes have gained increasing attention and valuable immunomodulatory properties have been attributed to the secretome of γ-irradiated peripheral blood mononuclear cells (PBMCs). Potential effects of the PBMC secretome (PBMCsec) on key DC functions have not been elucidated so far. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ebiom.2020.102774DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7218268PMC