431,452 results match your criteria Biotechnology reports Amsterdam Netherlands[Journal]


Electronic structure benchmark calculations of CO fixing elementary chemical steps in RuBisCO using the projector-based embedding approach.

J Comput Chem 2020 Jul 8. Epub 2020 Jul 8.

Departamento de Físico-Química, Facultad de Ciencias Químicas, Universidad de Concepción, Concepción, Chile.

Ribulose 1,5-bisphosphate carboxylase-oxygenase (RuBisCO) is the main enzyme involved in atmospheric carbon dioxide (CO ) fixation in the biosphere. This enzyme catalyzes a set of five chemical steps that take place in the same active-site within magnesium (II) coordination sphere. Here, a set of electronic structure benchmark calculations have been carried out on a reaction path proposed by Gready et al. Read More

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http://dx.doi.org/10.1002/jcc.26380DOI Listing

Low-dose biochar added to sediment improves water quality and promotes the growth of submerged macrophytes.

Sci Total Environ 2020 Jun 29;742:140602. Epub 2020 Jun 29.

Ministry of Water Resources Research Center of Poyang Lake Water Resources and Water Environment, Jiangxi Institute of Water Sciences, Nanchang 330029, PR China. Electronic address:

Biochar is a good adsorbent for water pollutants. However, the effects of biochar on aquatic organisms are not well understood. In this study, different amounts of biochar (CK, 0 mg/g; T1, 10 mg/g; T2, 30 mg/g) were added to sediment to study changes in water quality and its impact on three submerged macrophytes (Hydrilla verticillata, Vallisneria natans, and Ceratophyllum demersum) and the sediment microbial community. Read More

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http://dx.doi.org/10.1016/j.scitotenv.2020.140602DOI Listing

Analysis of genotoxic effects on plants exposed to high traffic volume in urban crossing intersections.

Chemosphere 2020 Jun 29;259:127511. Epub 2020 Jun 29.

Federal University of Uberlândia, Institute of Biotechnology, Umuarama Campus, Uberlândia, Minas Gerais, Brazil; Federal University of Uberlândia, Institute of Geography, Santa Mônica Campus, Avenida João Naves de Ávila, 2121, 38.408-100, Uberlândia, Minas Gerais, Brazil. Electronic address:

A biological assessment of environmental quality was performed using the tropical plant species Tradescantia pallida (Rose) D.R. Hunt. Read More

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http://dx.doi.org/10.1016/j.chemosphere.2020.127511DOI Listing

Analysis of the intricate effects of polyunsaturated fatty acids and polyphenols on inflammatory pathways in health and disease.

Food Chem Toxicol 2020 Jul 5:111558. Epub 2020 Jul 5.

Laboratory of Toxicology and Forensic Sciences, Medical School, University of Crete, 71003, Heraklion, Greece; Department of Analytical and Forensic Medical Toxicology, Sechenov University, 2-4 Bolshaya Pirogovskaya st, 119991, Moscow, Russia. Electronic address:

Prevention and treatment of non-communicable diseases (NCDs), including cardiovascular disease, diabetes, obesity, cancer, Alzheimer's and Parkinson's disease, arthritis, non-alcoholic fatty liver disease and various infectious diseases; lately most notably COVID-19 have been in the front line of research worldwide. Although targeting different organs, these pathologies have common biochemical impairments - redox disparity and, prominently, dysregulation of the inflammatory pathways. Research data have shown that diet components like polyphenols, poly-unsaturated fatty acids (PUFAs), fibres as well as lifestyle (fasting, physical exercise) are important factors influencing signalling pathways with a significant potential to improve metabolic homeostasis and immune cells' functions. Read More

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http://dx.doi.org/10.1016/j.fct.2020.111558DOI Listing

Modulating effect of Vitamin D3 on the mutagenicity and carcinogenicity of doxorubicin in Drosophila melanogaster and in silico studies.

Food Chem Toxicol 2020 Jul 5:111549. Epub 2020 Jul 5.

Institute of Biotechnology, Federal University of Uberlândia, Campus Umuarama, Uberlândia, MG, Brazil. Electronic address:

Vitamin D3 (VD3) deficiency increases DNA damage, while supplementation may exert a pro-oxidant activity, prevent viral infections and formation of tumors. The aim of this study was to investigate the mutagenicity and carcinogenicity of VD3 alone or in combination with doxorubicin (DXR) using the Somatic Mutation and Recombination Test and the Epithelial Tumor Test, both in Drosophila melanogaster. For better understanding of the molecular interactions of VD3 and receptors, in silico analysis were performed with molecular docking associated with molecular dynamics. Read More

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http://dx.doi.org/10.1016/j.fct.2020.111549DOI Listing

Antimicrobial peptides-loaded smart chitosan hydrogel: Release behavior and antibacterial potential against antibiotic resistant clinical isolates.

Int J Biol Macromol 2020 Jul 5. Epub 2020 Jul 5.

Cellular and Molecular Research Centre, Iran University of Medical Sciences, Tehran, Iran; Department of Tissue Engineering & Regenerative Medicine, Faculty of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, Iran. Electronic address:

In this study, we synthesized thermo-responsive chitosan (TCTS) hydrogels, and loaded with different concentrations of antimicrobial peptide (AMP) (0, 4, 8 and 16 μg·ml) to fabricate an antibacterial wound dressing against resistant clinical isolates. Physico-chemical properties, release behavior, cytobiocompatibility and antibacterial activity of the AMP-TCTS hydrogels against standard strain and resistant Acinetobacter baumannii were fully determined in vitro. The TCTS-40% β-glycerolphosphate hydrogels showed a gelation time of 15 min at 37 °C. Read More

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http://dx.doi.org/10.1016/j.ijbiomac.2020.07.011DOI Listing

Structure and dynamics analysis of a family 43 glycoside hydrolase α-L-arabinofuranosidase (PsGH43_12) from Pseudopedobacter saltans by computational modeling and small-angle X-ray scattering.

Int J Biol Macromol 2020 Jul 5. Epub 2020 Jul 5.

Carbohydrate Enzyme Biotechnology Laboratory, Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati 781039, Assam, India. Electronic address:

The structure and molecular dynamics of α-L-arabinofuranosidase (PsGH43_12) of family 43 glycoside hydrolase, subfamily 12 from Pseudopedobacter saltans were studied. The modeled PsGH43_12 structure displayed 5-bladed β-propeller fold at N-terminal and β-sandwich fold at C terminal. Ramachandran plot showed 95. Read More

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http://dx.doi.org/10.1016/j.ijbiomac.2020.07.007DOI Listing

Transcription factor LkWOX4 is involved in adventitious root development in Larix kaempferi.

Gene 2020 Jul 5:144942. Epub 2020 Jul 5.

State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Xiangshan Rd., Beijing 100091, China; Research Institute of Forestry, Chinese Academy of Forestry, Xiangshan Rd., Beijing 100091, China. Electronic address:

WUSCHEL-related homeobox4 (WOX4) plays important roles in vascular formation and adventitious root (AR) development. Here, we cloned the WOX4 from the AR of Larix kaempferi, whose cDNA is 1452 bp in length and encodes 483 amino acids. LkWOX4 is mainly expressed in the layer formation area of the stem at 10 days after cutting and its expression levels in the middles and ends of the ARs were higher than that in the AR tips. Read More

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http://dx.doi.org/10.1016/j.gene.2020.144942DOI Listing

A review of the toxicity in fish exposed to antibiotics.

Comp Biochem Physiol C Toxicol Pharmacol 2020 Jul 5:108840. Epub 2020 Jul 5.

Department of Food and Nutrition, Kookmin University, Seoul 02707, Republic of Korea. Electronic address:

Antibiotics are widely used in the treatment of human and veterinary diseases and are being used worldwide in the agriculture industry to promote livestock growth. However, a variety of antibiotics that are found in aquatic environments are toxic to aquatic organisms. Antibiotics are not completely removed by wastewater treatment plants and are therefore released into aquatic environments, which raises concern about the destruction of the ecosystem owing to their non-target effects. Read More

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http://dx.doi.org/10.1016/j.cbpc.2020.108840DOI Listing

Towards model-driven characterization and manipulation of plant lipid metabolism.

Prog Lipid Res 2020 Jul 5:101051. Epub 2020 Jul 5.

Genetics of Metabolic Traits Group, Max Planck Institute for Molecular Plant Physiology, Potsdam 14476, Germany; Department of Life Sciences, Ben-Gurion University of the Negev, 8410501 Beer-Sheva, Israel.

Plant lipids have versatile applications and provide essential fatty acids in human diet. Therefore, there has been a growing interest to better characterize the genetic basis, regulatory networks, and metabolic pathways that shape lipid quantity and composition. Addressing these issues is challenging due to context-specificity of lipid metabolism integrating environmental, developmental, and tissue-specific cues. Read More

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http://dx.doi.org/10.1016/j.plipres.2020.101051DOI Listing

The Mitotic Crosslinking Protein PRC1 Acts Like a Mechanical Dashpot to Resist Microtubule Sliding.

Dev Cell 2020 Jun 30. Epub 2020 Jun 30.

Department of Biological Sciences, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY 12180, USA. Electronic address:

Cell division in eukaryotes requires the regulated assembly of the spindle apparatus. The proper organization of microtubules within the spindle is driven by motor proteins that exert forces to slide filaments, whereas non-motor proteins crosslink filaments into higher-order motifs, such as overlapping bundles. It is not clear how active and passive forces are integrated to produce regulated mechanical outputs within spindles. Read More

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http://dx.doi.org/10.1016/j.devcel.2020.06.017DOI Listing

RNA Sensing by Gut Piezo1 Is Essential for Systemic Serotonin Synthesis.

Cell 2020 Jun 29. Epub 2020 Jun 29.

WPI Immunology Frontier Research Center (IFReC), Osaka University, Osaka 565-0871, Japan; Division of Cell Signaling, Okazaki Institute for Integrative Bioscience (National Institute for Physiological Sciences), National Institutes of Natural Sciences, Aichi 444-8787, Japan. Electronic address:

Gastrointestinal enterochromaffin cells regulate bone and gut homeostasis via serotonin (5-hydroxytryptamine [5-HT]) production. A recent report suggested that gut microbes regulate 5-HT levels; however, the precise underlying molecular mechanisms are unexplored. Here, we reveal that the cation channel Piezo1 in the gut acts as a sensor of single-stranded RNA (ssRNA) governing 5-HT production. Read More

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http://dx.doi.org/10.1016/j.cell.2020.06.022DOI Listing

Syringic acid modulates molecular marker-involved cell proliferation, survival, apoptosis, inflammation, and angiogenesis in DMBA-induced oral squamous cell carcinoma in Syrian hamsters.

J Biochem Mol Toxicol 2020 Jul 8:e22574. Epub 2020 Jul 8.

Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalai Nagar, India.

Despite, different medicinal phyto compounds giving an inexhaustible variety of anticancer drugs, potent signalling mechanism of leads  the key successes of anticancer agent, anti-inflammatory, induction of apoptosis, and antiangiogenic. The current study was conducted to estimate the effect of syringic acid (SA) on tumor necrosis factor-α (TNF-α)-mediated nuclear factor-κB (NF-κB) signaling pathways, inducing apoptosis and angiogenic signaling pathways in a hamster model by preneoplastic stages, histological, immunohistochemistry and immunoblots analysis. Hamsters were given oral cancer by painting 0. Read More

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http://dx.doi.org/10.1002/jbt.22574DOI Listing

Enzyme-catalysed polymer cross-linking: Biocatalytic tools for chemical biology, materials science and beyond.

Biopolymers 2020 Jul 8:e23390. Epub 2020 Jul 8.

School of Biochemistry, University of Bristol, University Walk, Bristol, UK.

Intermolecular cross-linking is one of the most important techniques that can be used to fundamentally alter the material properties of a polymer. The introduction of covalent bonds between individual polymer chains creates 3D macromolecular assemblies with enhanced mechanical properties and greater chemical or thermal tolerances. In contrast to many chemical cross-linking reactions, which are the basis of thermoset plastics, enzyme catalysed processes offer a complimentary paradigm for the assembly of cross-linked polymer networks through their predictability and high levels of control. Read More

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http://dx.doi.org/10.1002/bip.23390DOI Listing

Using unnatural amino acids to selectively label proteins for cellular imaging: A cell-biologist viewpoint.

Authors:
Natalie Elia

FEBS J 2020 Jul 8. Epub 2020 Jul 8.

Department of Life Sciences, Ben-Gurion University of the Negev, Beer Sheva, 84105, Israel.

Twenty-five years ago, green fluorescent protein (GFP) revolutionized the field of cell biology by enabling scientists to visualize, for the first time, proteins in living cells. However, when it comes to current, state-of-the-art imaging technologies, fluorescent proteins (such as GFP) have several limitations that result from their size and photophysics. Over the past decade, an elegant, alternative approach, which is based on the direct labeling of proteins with fluorescent dyes and is compatible with live-cell and super-resolution imaging applications, has been introduced. Read More

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http://dx.doi.org/10.1111/febs.15477DOI Listing

Characterization of two family AA9 LPMOs from Aspergillus tamarii with distinct activities on xyloglucan reveals structural differences linked to cleavage specificity.

PLoS One 2020 8;15(7):e0235642. Epub 2020 Jul 8.

Faculty of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences (NMBU), Ås, Norway.

Aspergillus tamarii grows abundantly in naturally composting waste fibers of the textile industry and has a great potential in biomass decomposition. Amongst the key (hemi)cellulose-active enzymes in the secretomes of biomass-degrading fungi are the lytic polysaccharide monooxygenases (LPMOs). By catalyzing oxidative cleavage of glycoside bonds, LPMOs promote the activity of other lignocellulose-degrading enzymes. Read More

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0235642PLOS

Drought-induced alterations in photosynthetic, ultrastructural and biochemical traits of contrasting sugarcane genotypes.

PLoS One 2020 8;15(7):e0235845. Epub 2020 Jul 8.

College of Agronomy and Biotechnology, China Agricultural University, Beijing, China.

Drought is an important factor which limits growth of sugarcane. To elucidate the physiological and biochemical mechanisms of tolerance, a pot experiment was conducted at Sugarcane Research Institute, Kaiyuan, China. Two genotypes (Yuetang 93-159-sensitive and Yunzhe 05-51-tolerant), were subjected to three treatments; 70±5% (control), 50±5% (moderate drought) and 30±5% (severe drought) of soil field capacity. Read More

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0235845PLOS
July 2020
3.234 Impact Factor

Highly Modified Lanostane Triterpenes from the Wood-Rot Basidiomycete : Comparative Chemical Investigations of Natural and Artificially Cultivated Fruiting Bodies and Mycelial Cultures.

J Nat Prod 2020 Jul 8. Epub 2020 Jul 8.

Institute of Quantum Beam Science, Ibaraki University, Mito 3101512, Japan.

The wood-rot basidiomycete has been chemically investigated. Comparative analyses of the natural fruiting body, artificially cultivated fruiting bodies, and mycelial cultures resulted in the isolation, in total, of 13 new highly modified lanostanes, ganocolossusins A-H (-) and ganodermalactones T-X (-), together with 23 known compounds (-). There were significant overlaps of the same compounds among the three different states of the fungal materials. Read More

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http://dx.doi.org/10.1021/acs.jnatprod.9b00947DOI Listing

Improving Enzyme Optimum Temperature Prediction with Resampling Strategies and Ensemble Learning.

J Chem Inf Model 2020 Jul 8. Epub 2020 Jul 8.

Accurate prediction of the optimal catalytic temperature (T) of enzymes is vital in biotechnology, as enzymes with high T values are desired for enhanced reaction rates. Recently, a machine-learning method (TOME) for predicting T was developed. TOME was trained on a normally-distributed dataset with a median T of 37°C and less than five percent of T values above 85°C, limiting the method's predictive capabilities for thermostable enzymes. Read More

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http://dx.doi.org/10.1021/acs.jcim.0c00489DOI Listing

WarmStart Colorimetric RT-LAMP for the rapid, sensitive and specific detection of Enteroviruses A-D targeting the 5'UTR region.

J Appl Microbiol 2020 Jul 8. Epub 2020 Jul 8.

University of Thessaly, School of Health Sciences, Department of Biochemistry & Biotechnology, Microbiology-Virology Laboratory, Biopolis, 41500, Larissa, Greece.

Aims: The aim of the present study was to develop a colorimetric LAMP assay for the detection of enteroviruses belonging to species A-D targeting the 5' untranslated region (5΄ UTR) of enteroviruses genome.

Methods And Results: The RNA was converted to cDNA by the reverse transcriptase and then amplified via LAMP by the WarmStart®Bst DNA polymerase, simultaneously in a single reaction tube, so we shortened the reaction time to 50 min. The sensitivity of the assay regarding Enterovirus B, C and D was determined to be 0. Read More

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http://dx.doi.org/10.1111/jam.14770DOI Listing

Interaction between CHOP and FoxO6 promotes hepatic lipid accumulation.

Liver Int 2020 Jul 8. Epub 2020 Jul 8.

Department of Pharmacy, College of Pharmacy, Pusan National University, 2, Busandaehak-ro 63beon-gi, Geumjeong-Gu, Busan, 46241, Korea.

Background & Aims: Endoplasmic reticulum (ER) stress is one of the major causes of hepatic insulin resistance through increasing de novo lipogenesis. Forkhead box O6 (FoxO6) is a transcription factor mediating insulin signaling to glucose and lipid metabolism, therefore, dysregulated FoxO6 is involved in hepatic insulin resistance. In this study, we elucidated the role of FoxO6 in ER stress-induced hepatic lipogenesis. Read More

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http://dx.doi.org/10.1111/liv.14594DOI Listing

Rapid adaptation for fibre degradation by changes in plasmid stoichiometry within Lactobacillus plantarum at the synthetic community level.

Microb Biotechnol 2020 Jul 8. Epub 2020 Jul 8.

Department of Life Sciences, National Institute for Biotechnology in the Negev, Ben-Gurion University of the Negev, Beer-Sheva, 8499000, Israel.

The multi-enzyme cellulosome complex can mediate the valorization of lignocellulosic biomass into soluble sugars that can serve in the production of biofuels and valuable products. A potent bacterial chassis for the production of active cellulosomes displayed on the cell surface is the bacterium Lactobacillus plantarum, a lactic acid bacterium used in many applications. Here, we developed a methodological pipeline to produce improved designer cellulosomes, using a cell-consortium approach, whereby the different components self-assemble on the surface of L. Read More

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http://dx.doi.org/10.1111/1751-7915.13584DOI Listing

Peroxiredoxin 2 deficiency reduces white adipogenesis due to the excessive ROS generation.

Cell Biol Int 2020 Jul 8. Epub 2020 Jul 8.

School of Life Sciences, BK21 Plus KNU Creative Bioresearch Group, Kyungpook National University, Daegu, Republic of Korea.

Reactive oxygen species (ROS) act as signaling molecules to regulate various cell functions. Numerous studies have demonstrated ROS to be essential for the differentiation of adipocytes. Peroxiredoxins (Prxs) are a ubiquitous family of antioxidant enzymes in mammalian cells. Read More

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http://dx.doi.org/10.1002/cbin.11417DOI Listing

Mutual environmental drivers of the community composition, functional attributes, and co-occurrence patterns of bacterioplankton in the composite aquatic ecosystem of Taihu watershed in China.

FEMS Microbiol Ecol 2020 Jul 8. Epub 2020 Jul 8.

State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, P.R.CHINA.

This study aimed to determine the environmental and ecological factors influencing the planktonic prokaryotic community profiles in the composite ecosystem comprising Taihu Lake, Taipu River, and Jinze Reservoir in the Taihu Watershed in China. A total of 42 water samples were intermittently collected from different sites in six months across four seasons. Physicochemical characteristics of the ecosystem, bacterioplankton diversity, and composition, the presence of co-occurrence patterns, and environmental predictors of ecological modules in the bacterioplankton network were determined. Read More

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http://dx.doi.org/10.1093/femsec/fiaa137DOI Listing

Mutations in the exocyst component EXOC2 cause severe defects in human brain development.

J Exp Med 2020 Oct;217(10)

Brain and Mitochondrial Research Group, Murdoch Children's Research Institute, Royal Children's Hospital, Melbourne, Victoria, Australia.

The exocyst, an octameric protein complex, is an essential component of the membrane transport machinery required for tethering and fusion of vesicles at the plasma membrane. We report pathogenic variants in an exocyst subunit, EXOC2 (Sec5). Affected individuals have severe developmental delay, dysmorphism, and brain abnormalities; variability associated with epilepsy; and poor motor skills. Read More

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http://dx.doi.org/10.1084/jem.20192040DOI Listing
October 2020

Immune responses of meningococcal B outer membrane vesicles in middle-aged mice.

Pathog Dis 2020 Jul 8. Epub 2020 Jul 8.

Department of Immunology, Adolfo Lutz Institute, Sao Paulo, Brazil.

The elderly are more likely to die when infected with Neisseria meningitidis. Aging is associated with immune system dysfunctions that impair responses to vaccines and infections. Therefore, immunization of middle-aged individuals could be beneficial. Read More

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http://dx.doi.org/10.1093/femspd/ftaa028DOI Listing

Antifungal Phenols from Collected in Oman.

J Nat Prod 2020 Jul 8. Epub 2020 Jul 8.

School of Pharmacy, Sungkyunkwan University, Suwon 16419, Republic of Korea.

is a flowering shrub unique to the Dhofar region of Oman and is used locally as a sedative and remedy for skin infection. However, no study to date has examined the pharmacological properties of this plant, and studies regarding phytochemicals present in are limited. Herein, phytochemical screening of the extract of was performed using LC/MS. Read More

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http://dx.doi.org/10.1021/acs.jnatprod.0c00395DOI Listing

Patterns of Avian Influenza A (H5) and A (H9) virus infection in backyard, commercial broiler and layer chicken farms in Bangladesh.

Transbound Emerg Dis 2020 Jul 8. Epub 2020 Jul 8.

School of Veterinary Science, University of Queensland, Gatton, Qld, Australia.

In order to control Highly Pathogenic Avian Influenza (HPAI) H5N1 and Low Pathogenic Avian Influenza (LPAI) H9N2 virus spread in endemically infected countries, a detailed understanding of infection patterns is required. We conducted cross-sectional studies in Bangladesh in 2016 and 2017, on 144 backyard, 106 broiler and 113 layer chicken farms. Although all sampled birds were negative for H5 virus by RT-PCR, H5 antibodies were detected in unvaccinated birds on all three farming systems. Read More

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http://dx.doi.org/10.1111/tbed.13657DOI Listing

Detection of the HLA-A*11:280 allele in a Taiwanese individual.

HLA 2020 Jul 8. Epub 2020 Jul 8.

Laboratory of Immunogenetics, Tzu Chi Cord Blood Bank, and Buddhist Tzu Chi Bone Marrow Donor Registry, Buddhist Tzu Chi Stem Cells Centre, Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Hualien, Taiwan, Republic of China.

One nucleotide substitution in codon 304 of HLA-A*11:01:01:01 results in a novel allele, HLA-A*11:280. This article is protected by copyright. All rights reserved. Read More

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http://dx.doi.org/10.1111/tan.13992DOI Listing

CCR5 deficiency/CCR5Δ32: resistant to HIV infection at the cost of curtailed CD4 T cell memory responses.

EMBO J 2020 Jul 8:e105854. Epub 2020 Jul 8.

Biotechnology Institute Thurgau (BITg), University of Konstanz, Kreuzlingen, Switzerland.

Orchestrated trafficking and activation by pathogen-derived peptides define the ability of CD4 T helper cells to contribute to an effective adaptive immunity. In this issue of The EMBO Journal, Martín-Leal et al show that the inflammatory chemokine receptor CCR5, well known for its role in cell migration and HIV infection, regulates ceramide synthesis and TCR nanoclustering to promote memory CD4 T cell activation. Read More

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http://dx.doi.org/10.15252/embj.2020105854DOI Listing

Viscoelastic tribopairs in dry and lubricated sliding friction.

Soft Matter 2020 Jul 8. Epub 2020 Jul 8.

Surface Science and Bio-nanomaterials Laboratory, Waterloo Institute for Nanotechnology, Institute for Polymer Research, Centre for Bioengineering and Biotechnology, Department of Chemical Engineering, University of Waterloo, 200 University Avenue West, Waterloo, Ontario, Canada N2L 3G1.

Soft contacts present different tribological responses compared to stiff materials, especially when soft materials exhibit viscoelastic behaviour, as viscoelastic materials have intermediate mechanical properties between viscous liquids and elastic solids. In this work, we investigated the influence of viscoelasticity of soft materials on sliding friction in dry and lubricated conditions. To achieve this, soft tribopairs with varying viscoelasticity were obtained by tuning the weight ratios of polydimethylsiloxane (PDMS) base and curing agent. Read More

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http://dx.doi.org/10.1039/d0sm00516aDOI Listing

Peptide-mediated porphyrin based hierarchical complexes for light-to-chemical conversion.

Nanoscale 2020 Jul 8. Epub 2020 Jul 8.

State Key Laboratory of Heavy Oil Processing and Center for Bioengineering and Biotechnology, China University of Petroleum, Qingdao 266580, China.

We present a new strategy for the biomimetic preparation of integrated photoactive complexes consisting of light harvesting and electron separation/transfer components via the hierarchical assembly of porphyrin and platinum nanoparticles on the surface of short-peptide self-assembled structures. The complexes can catalyze the conversion of visible light energy into chemical energy in the absence of an electron mediator and store it as nicotinamide adenine dinucleotide (NADH). This provides a promising step towards artificial photosystems through precisely controlled interactions of light-capturing agents, electron separators and biomolecules. Read More

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http://dx.doi.org/10.1039/d0nr03124kDOI Listing

Protein crystals as a key for deciphering macromolecular crowding effects on biological reactions.

Phys Chem Chem Phys 2020 Jul 8. Epub 2020 Jul 8.

ICBAS - Instituto de Ciências Biomédicas Abel Salazar, Universidade do Porto, Porto, Portugal and IBMC - Instituto de Biologia Molecular e Celular, Universidade do Porto, Porto, Portugal. and Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Porto, Portugal.

When placed in the same environment, biochemically unrelated macromolecules influence each other's biological function through macromolecular crowding (MC) effects. This has been illustrated in vitro by the effects of inert polymers on protein stability, protein structure, enzyme kinetics and protein aggregation kinetics. While a unified way to quantitatively characterize MC is still lacking, we show that the crystal solubility of lysozyme can be used to predict the influence of crowding agents on the catalytic efficiency of this enzyme. Read More

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http://dx.doi.org/10.1039/d0cp02469dDOI Listing

Building Communities-The 8th Congress of European Microbiologists.

Authors:
Michael Sauer

Biotechnol J 2020 Jul;15(7):e2000222

Institute of Microbiology and Microbial Biotechnology, Department of Biotechnology, BOKU-University of Natural Resources and Life Sciences, Vienna, 1180, Austria.

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http://dx.doi.org/10.1002/biot.202000222DOI Listing

The role of adipokines in the modulation of lymphoid lineage cell development and activity: An overview.

Obes Rev 2020 Jul 7. Epub 2020 Jul 7.

Faculty of Health Sciences, Department of Experimental Immunology, Medical University of Lodz, Lodz, Poland.

Adipokines are predominantly known to play a vital role in the control of food intake, energy homeostasis and regulation of glucose and lipid metabolism. However, evidence supporting the concept of their extensive involvement in immune system defence mechanisms and inflammatory processes continues to grow. Some of the adipokines, that is, leptin and resistin, have been recognized to exhibit mainly pro-inflammatory properties, whereas others such as visfatin, chemerin, apelin and vaspin have been found to exert regulatory effects. Read More

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http://dx.doi.org/10.1111/obr.13055DOI Listing

Leptin protects brain from ischemia/reperfusion-induced infarction by stabilizing the blood-brain barrier to block brain infiltration of the blood-borne neutrophils.

Eur J Neurosci 2020 Jul 7. Epub 2020 Jul 7.

Dept. of Life Sciences, National Chung Hsing University, Taichung City, 40227, Taiwan.

The cellular and molecular mechanisms underlying leptin-mediated brain protection against cerebral ischemia were investigated at the blood brain barrier (BBB) and neutrophil level. Through the ischemia/reperfusion (I/R) animal model, we found that leptin expression level was significantly decreased in ischemic hemisphere. Brain injection with leptin (15 μg/kg, intracisternally) could block the I/R-increased BBB permeability, activation of matrix metallopeptidase 9 (MMP-9) and brain infiltration of blood-borne neutrophils to reduce the infarct volume of ischemic brain. Read More

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http://dx.doi.org/10.1111/ejn.14896DOI Listing

Effect of cinnamon on migraine attacks and inflammatory markers: A randomized double-blind placebo-controlled trial.

Phytother Res 2020 Jul 7. Epub 2020 Jul 7.

Department of Community Nutrition, School of Nutrition and Food Science, Food Security Research Center, Isfahan University of Medical Sciences, Isfahan, Iran.

Migraine is the most common type of primary headaches. Increased levels of interleukin-6 (IL-6), calcitonin-gene-related peptide (CGRP) and nitric oxide (NO) lead to inflammation and neurogenic pain. Cinnamon has anti-inflammatory and neuroprotective properties. Read More

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http://dx.doi.org/10.1002/ptr.6721DOI Listing

Genetic and Transcriptomic Evidences Suggest ARO10 Genes Are Involved in Benzenoid Biosynthesis by Yeast.

Yeast 2020 Jul 7. Epub 2020 Jul 7.

Facultad de Quimica, Food Science and Technology Department, Enology and Fermentation Biotechnology Area, Universidad de la República, Uruguay.

Benzenoids are compounds associated with floral and fruity flavours in flowers, fruits and leaves and present a role in hormonal signalling in plants. These molecules are produced by the phenyl ammonia lyase pathway. However, some yeasts can also synthesize them from aromatic amino acids using an alternative pathway that remains unknown. Read More

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http://dx.doi.org/10.1002/yea.3508DOI Listing

Lifespan Extension Via Dietary Restriction: Time to Reconsider the Evolutionary Mechanisms?

Bioessays 2020 Jul 8:e1900241. Epub 2020 Jul 8.

Institute of Evolutionary Biology, School of Biological Science, University of Edinburgh, Edinburgh, EH9 3FL, UK.

Dietary restriction (DR) is the most consistent environmental manipulation to extend lifespan. Originally thought to be caused by a reduction in caloric intake, recent evidence suggests that macronutrient intake underpins the effect of DR. The prevailing evolutionary explanations for the DR response are conceptualized under the caloric restriction paradigm, necessitating reconsideration of how or whether these evolutionary explanations fit this macronutrient perspective. Read More

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http://dx.doi.org/10.1002/bies.201900241DOI Listing

Utility of Silane-Modified Magnesium-Based Magnetic Nanoparticles for Efficient Immobilization of Bacillus thermoamylovorans Lipase.

Appl Biochem Biotechnol 2020 Jul 8. Epub 2020 Jul 8.

Department of Physics, Himachal Pradesh University, Shimla, 171005, India.

Enzymes and protein's immobilization on magnetic nano supports is emerging as a promising candidate in the food, medical field, and areas of environmental studies. This work presents a study on purified Bacillus thermoamylovorans lipase (BTL) by utilizing tetraethoxysilane (TEOS)-modified magnesium nano ferrite (MgNF) of 20 nm size. Its structural and morphological studies were investigated by powder X-ray diffractometry, high-resolution transmission electron microscopy, etc. Read More

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http://dx.doi.org/10.1007/s12010-020-03379-7DOI Listing

Integrating Value Considerations in the Decision Making for the Design of Biorefineries.

Sci Eng Ethics 2020 Jul 7. Epub 2020 Jul 7.

Biotechnology Department, Delft University of Technology, Van der Maasweg 9, 2629HZ, Delft, The Netherlands.

Biobased production has been promoted as a sustainable alternative to fossil resources. However, controversies over its impact on sustainability highlight societal concerns, value tensions and uncertainties that have not been taken into account during its development. In this work, the consideration of stakeholders' values in a biorefinery design project is investigated. Read More

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http://dx.doi.org/10.1007/s11948-020-00251-zDOI Listing

Phylogenetic analysis of Anguilla marmorata population in Thua Thien Hue, Vietnam based on the cytochrome C oxidase I (COI) gene fragments.

AMB Express 2020 Jul 7;10(1):122. Epub 2020 Jul 7.

Faculty of Fisheries, University of Agriculture and Forestry, Hue University, 102 Phung Hung, Hue, 49000, Vietnam.

The giant mottled eel is a species with high commercial value so overfishing, river management, and water pollution have negatively affected its movement and population numbers. Anguilla marmorata (eel) was listed in the Vietnam Red Data Book 2007 with a description of Vulnerability. This study used a barcode technique to analyze molecular characteristics and build genetic plants based on the cytochrome c oxidase I gene segment isolated from the mitochondrial genome of 48 individuals of A. Read More

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http://dx.doi.org/10.1186/s13568-020-01059-7DOI Listing

The present and potential future methods for delivering CRISPR/Cas9 components in plants.

J Genet Eng Biotechnol 2020 Jul 7;18(1):25. Epub 2020 Jul 7.

Department of Biotechnology, UIET, Panjab University, Chandigarh, India.

Background: CRISPR/Cas9 genome editing technology is a DNA manipulation tool for trait improvement. This technology has been demonstrated and successfully applied to edit the genome in various species of plants. The delivery of CRISPR/Cas9 components within rigid plant cells is very crucial for high editing efficiency. Read More

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http://dx.doi.org/10.1186/s43141-020-00036-8DOI Listing

Enhancement of protoplast preparation and regeneration of Hirsutella sinensis based on process optimization.

Biotechnol Lett 2020 Jul 8. Epub 2020 Jul 8.

Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, 310014, People's Republic of China.

Objective: To explore the optimal methods for the protoplast preparation and regeneration of Hirsutella sinensis by optimizing the limiting factors.

Results: During the treatment of enzymatic protoplast preparation, mycelium cultured for 7 days was the optimal start material. The maximum protoplast preparation rate of 4. Read More

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http://dx.doi.org/10.1007/s10529-020-02958-2DOI Listing

Microscopic analysis of the microbiota of three commercial Phytoseiidae species (Acari: Mesostigmata).

Exp Appl Acarol 2020 Jul 7. Epub 2020 Jul 7.

Ecology and Evolutionary Biology Section, School of Biological Sciences, University of Reading, Reading, RG6 6AS, UK.

Microbes associated with the external and internal anatomy of three commercially available predatory mite species-Phytoseiulus persimilis, Typhlodromips (= Amblyseius) swirskii, and Neoseiulus (= Amblyseius) cucumeris-were examined using light microscopy, confocal laser scanning microscopy and fluorescence in-situ hybridization (FISH). Four microbe morphotypes were observed on external body regions. These included three microfungi-like organisms (named T1, T2 and T3) and rod-shaped bacteria (T4). Read More

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http://dx.doi.org/10.1007/s10493-020-00520-3DOI Listing

The specific PKC-α inhibitor chelerythrine blunts costunolide-induced eryptosis.

Apoptosis 2020 Jul 7. Epub 2020 Jul 7.

Department of Dermatology and Allergology, School of Medicine, Technical University of Munich, Biedersteinerstr. 29, 80802, München, Germany.

Costunolide, a natural sesquiterpene lactone, has multiple pharmacological activities such as neuroprotection or induction of apoptosis and eryptosis. However, the effects of costunolide on pro-survival factors and enzymes in human erythrocytes, e.g. Read More

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http://dx.doi.org/10.1007/s10495-020-01620-6DOI Listing

The gut and feed residue microbiota changing during the rearing of Hermetia illucens larvae.

Antonie Van Leeuwenhoek 2020 Jul 7. Epub 2020 Jul 7.

Institute of Applied Microbiology, Justus-Liebig University Giessen, IFZ-Heinrich-Buff-Ring 26-32, 35392, Giessen, Germany.

Larvae of Hermetia illucens, commonly known as black soldier fly, efficiently convert organic waste into nutrient-rich supplements for different applications. Here we performed a preliminary experiment to investigate the dynamics of the H. illucens gut microbiota and changes in the composition of the bacterial community in the residue of the larval feed during rearing. Read More

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http://dx.doi.org/10.1007/s10482-020-01443-0DOI Listing

Intestinal protozoan and helminthic infections among hemodialysis and cancer patients.

Parasitol Res 2020 Jul 8. Epub 2020 Jul 8.

University of Cologne, Faculty of Medicine and University Hospital, Cologne, Germany.

Intestinal parasitic infections (IPIs) can be a severe threat to immunocompromised patients. This is particularly true for those undergoing chemotherapy and hemodialysis. The present research is aimed at identifying intestinal parasites that might be present in immunocompromised patients. Read More

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http://dx.doi.org/10.1007/s00436-020-06774-5DOI Listing

Advances in histone deacetylase inhibitors in targeting glioblastoma stem cells.

Cancer Chemother Pharmacol 2020 Jul 7. Epub 2020 Jul 7.

CSIR-Centre for Cellular and Molecular Biology, Habsiguda, Uppal Road, Hyderabad, 500007, Telangana, India.

Glioblastoma multiforme (GBM) is a lethal grade IV glioma (WHO classification) and widely prevalent primary brain tumor in adults. GBM tumors harbor cellular heterogeneity with the presence of a small subpopulation of tumor cells, described as GBM cancer stem cells (CSCs) that pose resistance to standard anticancer regimens and eventually mediate aggressive relapse or intractable progressive GBM. Existing conventional anticancer therapies for GBM do not target GBM stem cells and are mostly palliative; therefore, exploration of new strategies to target stem cells of GBM has to be prioritized for the development of effective GBM therapy. Read More

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http://dx.doi.org/10.1007/s00280-020-04109-wDOI Listing

Protein intake and osteosarcopenic adiposity in Korean adults aged 50 years and older.

Authors:
M-K Choi Y-J Bae

Osteoporos Int 2020 Jul 8. Epub 2020 Jul 8.

Division of Food Science and Biotechnology, Korea National University of Transportation, Jeungpyeong, South Korea.

Intake of plant-based protein and recommended protein intake are associated with a lower risk of osteosarcopenic adiposity (co-occurrence of osteopenia/osteoporosis, sarcopenia, and adiposity) in elderly Korean men.

Introduction: Osteosarcopenic adiposity (OSA) syndrome is defined as the concurrent presence of osteopenia/osteoporosis, sarcopenia, and adiposity and leads to negative functional and metabolic outcomes in late adulthood. This study aimed to investigate the association between OSA and protein intake in adults aged 50 or older. Read More

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http://dx.doi.org/10.1007/s00198-020-05529-3DOI Listing