4,668 results match your criteria Biotechnology progress[Journal]


Repurposing fed-batch media and feeds for highly productive CHO perfusion processes.

Biotechnol Prog 2019 Apr 15:e2821. Epub 2019 Apr 15.

GE Healthcare Bio-Sciences AB, Björkgatan 30, 751 84 Uppsala, Sweden.

Perfusion is a cell culture mode that is gaining popularity for the manufacture of monoclonal antibodies and their derivatives. The cell culture media supporting perfusion culture need to support higher cell densities than those used in fed-batch culture. Therefore, when switching from a fed-batch to a perfusion mode, a new medium needs to be developed which supports high cell densities, high productivity and favorable product quality. Read More

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http://dx.doi.org/10.1002/btpr.2821DOI Listing

Anxa2- and Ctsd-knockout CHO cell lines to diminish the risk of contamination with host cell proteins.

Biotechnol Prog 2019 Apr 11:e2820. Epub 2019 Apr 11.

Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Higashi 1-1-1, Tsukuba, Ibaraki, Japan.

Chinese hamster ovary (CHO) cells have been used as host cells in the production of a range of recombinant therapeutic proteins, including monoclonal antibodies and Fc-fusion proteins. Host cell proteins (HCP) represent impurities that must be removed from therapeutic formulations because of their potential risks for immunogenicity. While the majority of HCP impurities are effectively removed in typical downstream purification processes, clearance of a small population of HCP remains challenging. Read More

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http://dx.doi.org/10.1002/btpr.2820DOI Listing

Heat-inactivated coelomic fluid of the earthworm Perionyx excavatus is a possible alternative source for Fetal Bovine Serum in animal cell culture.

Biotechnol Prog 2019 Apr 11:e2817. Epub 2019 Apr 11.

Molecular Nanomedicine Research Unit, Centre for Nanoscience & Nanotechnology, International Research Centre, Sathyabama Institute of Science & Technology, Chennai, Tamilnadu, India.

Fetal bovine serum (FBS) is used as a major supplement in culturing animal cells under in-vitro conditions. Due to ethical concern, high cost, biosafety and geographical as well as batch wise result variations, it is important to reduce or replace the use of FBS in animal cell culture. The major objective of this work is to evaluate the feasibility of heat-inactivated coelomic fluid (HI-CF) of the earthworm, Perionyx excavatus as a possible alternative for FBS in animal cell culture experiments. Read More

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http://dx.doi.org/10.1002/btpr.2817DOI Listing

Comparison of Expression Optimization of New Derivative of Staphylokinase (SAK-2RGD-TTI) with the rSAK.

Biotechnol Prog 2019 Apr 11:e2819. Epub 2019 Apr 11.

Biotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran.

Staphylokinase (SAK) is a promising thrombolytic agent for the treatment of patients suffering from blood-clotting disorders. To increase the potency of SAK and to minimize vessel reocclusion, a new construct bearing SAK motif fused to tsetse thrombin inhibitor (TTI) via a 20-amino acid linker with 2 RGD (2 x Arginine-Glycine-Aspartic acid inhibiting platelet aggregation via attachment to integrin receptors of platelet) was codon optimized and expressed comparatively in Pichia pastoris GS115 as a Mut strain and KM71H as a Mut strain. Fusion protein was optimized in terms of best expression condition and fibrinolytic activity and compared with the rSAK. Read More

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http://dx.doi.org/10.1002/btpr.2819DOI Listing
April 2019
1 Read

Effects of phosphonium-based ionic liquids on the lipase activity evaluated by experimental results and molecular docking.

Biotechnol Prog 2019 Apr 10:e2816. Epub 2019 Apr 10.

Universidade Tiradentes, Av. Murilo Dantas 300, Farolândia, 49032-490, Aracaju, SE, Brazil.

In this work, the effect of several phosphonium-based ionic liquids (ILs) on the activity of lipase from Burkholderia cepacia (BCL) was evaluated by experimental assays and molecular docking. ILs comprising different cations ([P ] , [P ] , [P ] ) and anions (Cl , Br , [Deca] , [Phosp] , [NTf ]-) were investigated to appraise the individual roles of IL ions on the BCL activity. From the activity assays, it was found that an increase in the cation alkyl chain length leads to a decrease on the BCL enzymatic activity. Read More

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http://dx.doi.org/10.1002/btpr.2816DOI Listing

Decision Tree - PLS (DT - PLS) algorithm for the development of process - specific local prediction models.

Biotechnol Prog 2019 Apr 10:e2818. Epub 2019 Apr 10.

Institute of Chemical and Bioengineering, Department of Chemistry and Applied Biosciences, ETH Zurich, Switzerland.

This work presents a novel multivariate statistical algorithm, DT - PLS, to improve the prediction and understanding of dynamic processes based on local partial least square regression (PLSR) models for characteristic process groups defined based on decision tree (DT) analysis. The DT-PLS algorithm is successfully applied to two different cell culture datasets, one obtained from bioreactors of 3.5L lab scale and the other obtained from the 15 mL ambr micro-bioreactor system. Read More

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http://dx.doi.org/10.1002/btpr.2818DOI Listing

A novel method for constructing an acellular 3D biomatrix from bovine spinal cord for neural tissue engineering applications.

Biotechnol Prog 2019 Apr 8:e2814. Epub 2019 Apr 8.

Regenerative Biomaterials Laboratory, Department of Bioengineering, Engineering Faculty, Canakkale Onsekiz Mart University, Turkey.

In this study, we aimed at generating 3-dimensional (3D) decellularized bovine spinal cord extracellular matrix-based scaffolds (3D-dCBS) for neural tissue engineering applications. Within this scope, bovine spinal cord (BSC) tissue pieces were homogenized in 0.1 M NaOH and this viscous mixture was molded to attain 3D bioscaffolds. Read More

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http://dx.doi.org/10.1002/btpr.2814DOI Listing

Experiment and simulation on kinetic resolution of (R,S)-2-chloromandelic acid by enzymatic transesterification.

Biotechnol Prog 2019 Apr 8:e2815. Epub 2019 Apr 8.

Dept. of Chemistry and Chemical Engineering, Hunan Institute of Science and Technology, Yueyang 414006, Hunan, China.

Optically pure 2-chloromandelic acid (ClMA) is a very important chiral drug intermediate for synthesis of (S)-clopidogrel, belonging to the platelet aggregation inhibitor. Enantioselective resolution of (R,S)-2-chloromandelic acid was carried out in organic solvent through irreversible transesterification catalyzed by lipase AK with vinyl acetate acting as the acyl donor. Effects of various conditions on enantioselectivity and activity of lipase were investigated, including organic solvents, temperature, water content, substrate ratio, enzyme loading and reaction time. Read More

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https://onlinelibrary.wiley.com/doi/abs/10.1002/btpr.2815
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http://dx.doi.org/10.1002/btpr.2815DOI Listing
April 2019
9 Reads

Multistage continuous countercurrent diafiltration for formulation of monoclonal antibodies.

Biotechnol Prog 2019 Apr 4:e2810. Epub 2019 Apr 4.

Department of Chemical Engineering, The Pennsylvania State University, University Park, Pennsylvania.

There is growing interest in the development of fully integrated and continuous biomanufacturing processes for the production of monoclonal antibody products. A recent study has demonstrated the feasibility of using a two-stage countercurrent diafiltration (DF) process for continuous product formulation, but this system did not provide sufficient levels of buffer exchange for most applications. The objective of this study was to design and test a three-stage countercurrent DF system that could achieve at least 99. Read More

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http://dx.doi.org/10.1002/btpr.2810DOI Listing

Model-based monitoring of industrial reversed phase chromatography to predict insulin variants.

Biotechnol Prog 2019 Apr 1:e2813. Epub 2019 Apr 1.

Division of Biotechnology, Department of Physics, Chemistry and Biology, Linköping University, Linköping, Sweden.

Downstream processing in the manufacturing biopharmaceutical industry is a multistep process separating the desired product from process- and product-related impurities. However, removing product-related impurities, such as product variants, without compromising the product yield or prolonging the process time due to extensive quality control analytics, remains a major challenge. Here, we show how mechanistic model-based monitoring, based on analytical quality control data, can predict product variants by modeling their chromatographic separation during product polishing with reversed phase chromatography. Read More

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http://dx.doi.org/10.1002/btpr.2813DOI Listing

Optimization of continuous purification of recombinant patchoulol synthase from Escherichia coli with membrane adsorbers.

Biotechnol Prog 2019 Apr 1:e2812. Epub 2019 Apr 1.

Institut für Technische Chemie, Leibniz Universität Hannover, Callinstraße 5, 30167 Hannover, Germany.

The natural production of patchouli oil in developing countries cannot meet the increasing demand any more. This leads to socioecological consequences, such as the use of arable land, which is actually intended for food. Hence, the world market price increased up to $150/kg. Read More

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http://dx.doi.org/10.1002/btpr.2812DOI Listing

Development of a Novel, High-throughput Screening Tool for Efficient Perfusion-based Cell Culture Process Development.

Biotechnol Prog 2019 Apr 1:e2811. Epub 2019 Apr 1.

Upstream Development, Bioprocess Development, Shire, 300 Shire Way, Lexington, MA.

Perfusion technology has been successfully used for the commercial production of biotherapeutics, in particular unstable recombinant proteins, for more than a decade. However, there has been a general lack of high-throughput cell culture tools specifically for perfusion-based cell culture processes. Here we have developed a high-throughput cell retention operation for use with the ambr® 15 bioreactor system. Read More

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http://dx.doi.org/10.1002/btpr.2811DOI Listing

Silver-based surface enhanced Raman spectroscopy devices for detection of organophosphorus pesticides traces.

Biotechnol Prog 2019 Mar 21:e2809. Epub 2019 Mar 21.

RENORBIO, Biotechnology Graduate Program, Universidade Federal Rural de Pernambuco, Recife, Pernambuco, Brazil.

The detection of traces of substances by surface-sensitive techniques such as surface enhanced Raman spectroscopy (SERS) explores the interaction of adsorbed molecules on plasmonic surfaces to improve the limit of detection of analytes. This article is an overview about recent development in SERS substrates applied in the detection of organophosphorus pesticides on plasmonic surfaces (arrays of metal nanoparticles). The morphology, roughness, chemical functionalization degree, and aggregation level of plasmonic centers are some of the critical parameters to be controlled in the optimization of SERS signal from specific analytes. Read More

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http://dx.doi.org/10.1002/btpr.2809DOI Listing
March 2019
4 Reads

Effects of preheating on briquetting and subsequent hydrothermal pretreatment for enzymatic saccharification of wheat straw.

Biotechnol Prog 2019 Mar 19:e2808. Epub 2019 Mar 19.

Department of Geosciences and Natural Resource Management, University of Copenhagen, Frederiksberg C, Denmark.

Briquetting of plant biomass with low bulk density is an advantage for handling, transport, and storage of the material, and heating of the biomass prior to the briquetting facilitates the densification process and improves the physical properties of the briquettes. This study investigates the effects of preheating prior to briquetting of wheat straw (WS) on subsequent hydrothermal pretreatment and enzymatic conversion to fermentable sugars. WS (11% moisture content) was densified to briquettes under different conditions; without preheating or with preheating at 75 or 125°C for either 5 or 10 min. Read More

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http://dx.doi.org/10.1002/btpr.2808DOI Listing

Hybrid coating of alginate microbeads based on protein-biopolymer multilayers for encapsulation of probiotics.

Biotechnol Prog 2019 Mar 18:e2806. Epub 2019 Mar 18.

University of Copenhagen, Department of Food Science, Copenhagen, Denmark.

A hybrid coating based on multilayers of proteins and biopolymers was developed to enhance the protection performance of alginate microbeads against acidic conditions for delivery of probiotics (Lactobacillus rhamnosus GG). Zeta potential measurements and quartz crystal microbalance with dissipation confirmed layer-by-layer deposition of protein-polymer layers. The stability of protein-based coatings during simulated gastric fluid (SGF) treatment was monitored by microscopy. Read More

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http://dx.doi.org/10.1002/btpr.2806DOI Listing

Cross-linked enzyme aggregates of recombinant Candida antarctica lipase B for the efficient synthesis of olvanil, a nonpungent capsaicin analogue.

Biotechnol Prog 2019 Mar 18:e2807. Epub 2019 Mar 18.

Biotecnología Industrial, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, CIATEJ, Zapopan, Jalisco, Mexico.

Despite the proven therapeutic role of capsaicin in human health, its usage is still hampered by its high pungency. In this sense, nonpungent capsaicin analogues as olvanil are a feasible alternative to the unpleasant sensations produced by capsaicin while maintaining a similar pharmacological profile. Olvanil can be obtained by a lipase-catalyzed chemoenzymatic process. Read More

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http://dx.doi.org/10.1002/btpr.2807DOI Listing
March 2019
1 Read

Identification of upstream culture conditions and harvest time parameters that affect host cell protein clearance.

Biotechnol Prog 2019 Mar 13:e2805. Epub 2019 Mar 13.

Department of Biochemical Engineering, University College London, London, UK.

During early stage bioprocess development, characterizing interactions between unit operations is a key challenge. Such interactions include the release of host cell enzymes early in the process causing losses in product quality downstream. Using a CHO-expressed IgG1 system, the impact of cell culture duration was investigated using a 50 L bioreactor and performing scale-down protein A purification. Read More

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http://doi.wiley.com/10.1002/btpr.2805
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http://dx.doi.org/10.1002/btpr.2805DOI Listing
March 2019
12 Reads

Carboxylic acid consumption and production by Corynebacterium glutamicum.

Biotechnol Prog 2019 Mar 9:e2804. Epub 2019 Mar 9.

Department of Biotechnology, Bioprocess Engineering, Technische Universität Berlin, Berlin, Germany.

Corynebacterium glutamicum is well-known as an industrial workhorse, most notably for its use in the bulk production of amino acids in the feed and food sector. Previous studies of the effect of gradients in scale-down reactors with complex media disclosed an accumulation of several carboxylic acids and a parallel decrease of growth and product accumulation. This study, therefore, addresses the impact of carboxylic acids, for example, acetate and l-lactate, on the cultivation of the cadaverine producing strain C. Read More

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http://dx.doi.org/10.1002/btpr.2804DOI Listing

Multiple response optimization of alkaline pretreatment of sisal fiber (Agave sisalana) assisted by ultrasound.

Biotechnol Prog 2019 Mar 6:e2802. Epub 2019 Mar 6.

Instituto de Química, Campus Universitário de Ondina, Universidade Federal da Bahia, Salvador, Bahia, Brazil.

A procedure for the alkaline pretreatment of sisal fiber assisted by ultrasound was optimized to obtain a higher solubilization of hemicellulose and the removal of lignin with cellulose fraction maintenance. A full factorial design 2 was used for the evaluation of the effects of the variables (sonication time, NaOH concentration, and sonication amplitude) on the pretreatment. The optimal values for the variables using the Doehlert matrix for the sonication time, NaOH concentration, and sonication amplitude were 27 min, 4. Read More

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http://dx.doi.org/10.1002/btpr.2802DOI Listing

Dihydroxyacetone production from glycerol using Gluconobacter oxydans: Study of medium composition and operational conditions in shaken flasks.

Biotechnol Prog 2019 Mar 6:e2803. Epub 2019 Mar 6.

Department of Chemical and Materials Engineering, Faculty of Chemistry, Universidad Complutense de Madrid, Madrid, Spain.

The production of dihydroxyacetone from glycerol employing aerobic cultures of Gluconobacter oxydans is studied. Dihydroxyacetone is one of the most important value-added products obtained from glycerol, a by-product of biodiesel production. The effect of organic nitrogen source and initial substrate concentrations has been studied together with the possibility of product inhibition. Read More

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http://dx.doi.org/10.1002/btpr.2803DOI Listing

Optimization of pH induced flocculation of marine and freshwater microalgae via central composite design.

Biotechnol Prog 2019 Mar 6:e2801. Epub 2019 Mar 6.

Bioengineering Department, Yıldız Technical University, Istanbul, Turkey.

Microalgae harvesting via pH induced flocculation along with utilization of recovered medium after flocculation is one of the most economical methods for separating the microalgal biomass in order to reduce the dewatering cost. In this study, optimization of marine and freshwater microalgae flocculation by pH adjustment was investigated via central composite design methodology. One molar of KOH and NaOH solutions were used to increase the pH level of the microalgal culture. Read More

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http://dx.doi.org/10.1002/btpr.2801DOI Listing

Expansion of retinal stem cells and their progeny using cell microcarriers in a bioreactor.

Biotechnol Prog 2019 Mar 6:e2800. Epub 2019 Mar 6.

Institute of Medical Science, University of Toronto, Toronto, Ontario, Canada.

Blindness as a consequence of degenerative eye diseases (e.g., age-related macular degeneration and retinitis pigmentosa) is a major health problem and numbers are expected to increase by up to 50% by 2020. Read More

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http://dx.doi.org/10.1002/btpr.2800DOI Listing

Efficient Itaconic acid production via protein-protein scaffold introduction between GltA, AcnA, and CadA in recombinant Escherichia coli.

Biotechnol Prog 2019 Mar 3:e2799. Epub 2019 Mar 3.

Department of Chemical Engineering, University of Ulsan, Ulsan, Republic of Korea.

Itaconic acid, which is a promising organic acid in synthetic polymers and some base-material production, has been produced by Aspergillus terreus fermentation at a high cost. The recombinant Escherichia coli that contained the cadA gene from A. terreus can produce itaconic acid but with low yield. Read More

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http://dx.doi.org/10.1002/btpr.2799DOI Listing
March 2019
4 Reads

Nutritional optimization of Arthrospira platensis for starch and Total carbohydrates production.

Biotechnol Prog 2019 Mar 3:e2798. Epub 2019 Mar 3.

Bioprocess Technology Division, School of Industrial Technology, Universiti Sains Malaysia, Penang, Malaysia.

Present study aims to optimize the production of starch and total carbohydrates from Arthrospira platensis. Growing concerns toward unprecedented environmental issues associated with plastic pollution has created a tremendous impetus to develop new biomaterials for the production of bioplastic. Starch-based biopolymers from algae serve as sustainable feedstock for thermoplastic starch production due to their abundant availability and low cost. Read More

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https://onlinelibrary.wiley.com/doi/abs/10.1002/btpr.2798
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http://dx.doi.org/10.1002/btpr.2798DOI Listing
March 2019
2 Reads

Amberlite IRA 900 versus calcium alginate in immobilization of a novel, engineered β-fructofuranosidase for short-chain fructooligosaccharide synthesis from sucrose.

Biotechnol Prog 2019 Feb 28:e2797. Epub 2019 Feb 28.

Department of Process Engineering, Stellenbosch University, Stellenbosch, South Africa.

The immobilization of β-fructofuranosidase for short-chain fructooligosaccharide (scFOS) synthesis holds the potential for a more efficient use of the biocatalyst. However, the choice of carrier and immobilization technique is a key to achieving that efficiency. In this study, calcium alginate (CA), Amberlite IRA 900 (AI900) and Dowex Marathon MSA (DMM) were tested as supports for immobilizing a novel engineered β-fructofuranosidase from Aspergillus japonicus for scFOS synthesis. Read More

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http://dx.doi.org/10.1002/btpr.2797DOI Listing
February 2019

Design of a low-cost culture medium based in whey permeate for biomass production of enological Lactobacillus plantarum strains.

Biotechnol Prog 2019 Feb 27:e2791. Epub 2019 Feb 27.

Laboratorio de Microbiología Molecular, Instituto de Microbiología Básica y Aplicada (IMBA), Departamento de Ciencia y Tecnología, Universidad Nacional de Quilmes, Bernal, Argentina.

The production of malolactic starter cultures requires the obtention of suitably large biomass at low-cost. In this work it was possible to obtain a good amount of biomass, at laboratory scale, of two enological strains of Lb. plantarum, by formulating a culture medium based on whey permeate (WP), a by-product of the cheese industry usually disposed as waste, when this was supplemented with yeast extract (Y), salts (S) and Tween 80 (T) (WPYST). Read More

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http://dx.doi.org/10.1002/btpr.2791DOI Listing
February 2019
2 Reads

Development of a rapid and reliable analytical method for screening poloxamer 188 for use in cell culture process.

Biotechnol Prog 2019 Feb 27:e2792. Epub 2019 Feb 27.

Materials Science, Biogen, Davis, North Carolina.

Poloxamer P188 is a common nonionic surfactant additive used in cell culture media as a cellular protectant from the hydrodynamic forces and shear stress during bioprocessing. Presence of a hydrophobic high molecular weight impurity contaminant has been shown to compromise its protective properties and lead to batch failure. In this work we present, a reliable, sensitive, and rapid analytical method to detect and quantify the contaminant impurity in poloxamer 188. Read More

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http://dx.doi.org/10.1002/btpr.2792DOI Listing
February 2019

Heterologous expression of Neurospora crassa cbh1 gene in Pichia pastoris resulted in production of a neutral cellobiohydrolase I.

Biotechnol Prog 2019 Feb 27:e2795. Epub 2019 Feb 27.

College of Life Sciences, Northwest A&F University, Yangling, Shaanxi, China.

The high production cost of cellulase is one of the limitations that hinder the commercialization of lignocellulose-based biorefineries. As one of the important cellulases, Neurospora crassa cellulase is not so intensively investigated as T. reesei cellulase. Read More

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http://dx.doi.org/10.1002/btpr.2795DOI Listing
February 2019
4 Reads

The effect of acetate on population heterogeneity in different cellular characteristics of Escherichia coli in aerobic batch cultures.

Biotechnol Prog 2019 Feb 28:e2796. Epub 2019 Feb 28.

Department of Chemical and Biochemical Engineering, Technical University of Denmark, Kongens Lyngby, Denmark.

Acetate as the major by-product in industrial-scale bioprocesses with Escherichia coli is found to decrease process efficiency as well as to be toxic to cells, which has several effects like a significant induction of cellular stress responses. However, the underlying phenomena are poorly explored. Therefore, we studied time-resolved population heterogeneity of the E. Read More

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http://dx.doi.org/10.1002/btpr.2796DOI Listing
February 2019
2 Reads

A novel nicotinamide adenine dinucleotide control strategy for increasing the cell density of Haemophilus parasuis.

Biotechnol Prog 2019 Feb 27:e2794. Epub 2019 Feb 27.

Post-doctoral Scientific Research Workstation, Shandong Binzhou Animal Science and Veterinary Medicine Academy, Binzhou, China.

Haemophilus parasuis is the causative agent of Glässer's disease and is a major source of economic losses in the swine industry each year. To enhance the production of an inactivated vaccine against H. parasuis, the availability of nicotinamide adenine dinucleotide (NAD) must be carefully controlled to ensure a sufficiently high cell density of H. Read More

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http://dx.doi.org/10.1002/btpr.2794DOI Listing
February 2019

Production of 2,3-butanediol by Klebsiella pneumoniae BLh-1 and Pantoea agglomerans BL1 cultivated in acid and enzymatic hydrolysates of soybean hull.

Biotechnol Prog 2019 Feb 27:e2793. Epub 2019 Feb 27.

Biotechnology & Biochemical Engineering Laboratory (BiotecLab), Federal University of Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil.

We investigated the production of 2,3-butanediol by two enterobacteria isolated from an environmental consortium, Klebsiella pneumoniae BLh-1 and Pantoea agglomerans BL1, in a bioprocess using acid and enzymatic hydrolysates of soybean hull as substrates. Cultivations were carried out in orbital shaker under microaerophilic conditions, at 30°C and 37°C, for both bacteria. Both hydrolysates presented high osmotic pressures, around 2,000 mOsm/kg, with varying concentrations of glucose, xylose, and arabinose. Read More

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http://dx.doi.org/10.1002/btpr.2793DOI Listing
February 2019
2 Reads

Semi-continuous scale-down models for clone and operating parameter screening in perfusion bioreactors.

Biotechnol Prog 2019 Feb 18:e2790. Epub 2019 Feb 18.

Department of Chemistry and Applied Biosciences, Institute for Chemical and Bioengineering, ETH Zürich, Zürich, Switzerland.

Perfusion cell culture, confined traditionally to the production of fragile molecules, is currently gaining broader attention in the biomanufacturing of therapeutic proteins. The development of these processes is made difficult by the limited availability of appropriate scale-down models. This is due to the continuous operation that requires complex control and cell retention capacity. Read More

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http://dx.doi.org/10.1002/btpr.2790DOI Listing
February 2019
1 Read

Effect of dilute acid pretreatment on the saccharification and fermentation of rye straw.

Biotechnol Prog 2019 Feb 18:e2789. Epub 2019 Feb 18.

Department of Spirit and Yeast Technology, Institute of Fermentation Technology and Microbiology, Faculty of Biotechnology and Food Sciences, Lodz University of Technology, Lodz, Poland.

This research shows the effect of dilute acid pretreatment with various sulfuric acid concentrations (0.5-2.0% [wt/vol]) on enzymatic saccharification and fermentation yield of rye straw. Read More

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http://dx.doi.org/10.1002/btpr.2789DOI Listing
February 2019

Mass spectrometric evaluation of upstream and downstream process influences on host cell protein patterns in biopharmaceutical products.

Biotechnol Prog 2019 Feb 15:e2788. Epub 2019 Feb 15.

Department of Analytical Development Biologicals, Boehringer Ingelheim Pharma GmbH & Co. KG, Analytical Development Biologicals, Biberach, Germany.

For production of different monoclonal antibodies (mAbs), biopharmaceutical companies often use related upstream and downstream manufacturing processes. Such platforms are typically characterized regarding influence of upstream and downstream process (DSP) parameters on critical quality attributes (CQAs). CQAs must be monitored strictly by an adequate control strategy. Read More

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http://dx.doi.org/10.1002/btpr.2788DOI Listing
February 2019
4 Reads

Apoptosis and cell cycle arrest of hepatocellular carcinoma spheroids treated by an alternating electric field.

Biotechnol Prog 2019 Feb 13:e2787. Epub 2019 Feb 13.

Department of Radiation Oncology, Chang Gung Memorial Hospital, Linkou, Taiwan.

Most of the current cancer therapies may induce serious side effects and affect patient quality of life. Recently, a novel treatment using an alternating low-intensity and intermediate-frequency electric field was proposed and found to be a noninvasive and minimally toxic approach. However, additional fundamental studies and scientific evidence are required to further support the development of this treatment into a standard cancer therapy. Read More

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http://dx.doi.org/10.1002/btpr.2787DOI Listing
February 2019

Transient gene expression using valproic acid in combination with co-transfection of SV40 large T antigen and human p21 /p27.

Biotechnol Prog 2019 Feb 13:e2786. Epub 2019 Feb 13.

R&D Cell Culture Technology of Centralized and Point of Care Solutions, Roche Diagnostics GmbH, Penzberg, Germany.

Transient gene expression (TGE) in HEK293 cells was optimized by Vink et al. by co-expression of human cell cycle inhibitors p21 /p27 and Simian virus 40 large T antigen (SVLT). In this study, we investigated the effect of this enhancer protein complex on the TGE experiments in a cell-cycle arrested condition of HEK293F cells induced by valproic acid. Read More

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http://doi.wiley.com/10.1002/btpr.2786
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http://dx.doi.org/10.1002/btpr.2786DOI Listing
February 2019
4 Reads

Using CFD simulations and statistical analysis to correlate oxygen mass transfer coefficient to both geometrical parameters and operating conditions in a stirred-tank bioreactor.

Biotechnol Prog 2019 Feb 13:e2785. Epub 2019 Feb 13.

Chemical Engineering, Oklahoma State University, Stillwater, Oklahoma.

Optimization of a bioreactor design can be an especially challenging process. For instance, testing different bioreactor vessel geometries and different impeller and sparger types, locations, and dimensions can lead to an exceedingly large number of configurations and necessary experiments. Computational fluid dynamics (CFD), therefore, has been widely used to model multiphase flow in stirred-tank bioreactors to minimize the number of optimization experiments. Read More

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http://dx.doi.org/10.1002/btpr.2785DOI Listing
February 2019

Balancing the effect of pretreatment severity on hemicellulose extraction and pulping performance during auto-hydrolysis prior to kraft pulping of acacia wood.

Biotechnol Prog 2019 Feb 12:e2784. Epub 2019 Feb 12.

Liaoning Key Laboratory of Pulp and Paper Engineering, Dalian Polytechnic University, Dalian, 116034, China.

When using a combination of pre-extraction and chemical pulping, a high yield of sugar recovery and minimal negative effect on the subsequent pulping step are expected. In this work, the P factor was utilized to investigate the effect of auto-hydrolysis severity on sugar recovery, removal of the main component, and impact on the kraft pulping of acacia wood chips. Using a P factor of 235, 84. Read More

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http://dx.doi.org/10.1002/btpr.2784DOI Listing
February 2019
1 Read

Biosorption of lanthanum and samarium by viable and autoclaved mycelium of Botryosphaeria rhodina MAMB-05.

Biotechnol Prog 2019 Feb 9:e2783. Epub 2019 Feb 9.

Departamento de Química, CCE, Universidade Estadual de Londrina, Londrina, Paraná, Brazil.

The ascomycetous fungus, Botryosphaeria rhodina MAMB-05, secretes a (1→3)(1→6)-β-D-glucan, and the scaled-up production of this β-glucan results in large amounts of mycelial biomass being produced that represent a potentially cost-effective biosorbent for rare-earth elements. High sorption capacities for both La(III) and Sm(III) were demonstrated for viable and autoclaved lyophilized fungal mycelium. Fourier-transformed infra-red absorption spectra and the point of zero charge (PZC) were similar for the viable and inactive fungal mycelia. Read More

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http://dx.doi.org/10.1002/btpr.2783DOI Listing
February 2019
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Case study: The characterization and implementation of dielectric spectroscopy (biocapacitance) for process control in a commercial GMP CHO manufacturing process.

Biotechnol Prog 2019 Feb 1:e2782. Epub 2019 Feb 1.

Cell Culture Development, Biogen, Research Triangle Park, North Carolina.

Dielectric spectroscopy (biocapacitance) is an up-and-coming technology for real time monitoring of biomass in cell culture processes and has opened the door for next-generation cell culture process control techniques such as automated on-demand nutrient feeding. In this case study we empirically demonstrate the lower limit of quantitation (LOQ), probe-to-probe consistency, and scalability of in situ biocapacitance probes using data generated from small- and large-scale Chinese hamster ovary (CHO) bioreactor cultures. The process understanding experiments culminated in the use of biocapacitance for process control in the current good manufacturing practices (GMP) manufacturing environment, first to automate the dilution of seed train cultures during scale-up stages and later as a method of predicting future glucose demand. Read More

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http://doi.wiley.com/10.1002/btpr.2782
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http://dx.doi.org/10.1002/btpr.2782DOI Listing
February 2019
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Comprehensive studies on optimization of cellulase and xylanase production by a local indigenous fungus strain via solid state fermentation using oil palm frond as substrate.

Biotechnol Prog 2019 Jan 30:e2781. Epub 2019 Jan 30.

Bioprocess Technology Division, School of Industrial Technology, Universiti Sains Malaysia, Penang, Malaysia.

The high cost of cellulases remains the most significant barrier to the economical production of bio-ethanol from lignocellulosic biomass. The goal of this study was to optimize cellulases and xylanase production by a local indigenous fungus strain (Aspergillus niger DWA8) using agricultural waste (oil palm frond [OPF]) as substrate. The enzyme production profile before optimization indicated that the highest carboxymethyl cellulose (CMCase), filter paper (FPase), and xylanase activities of 1. Read More

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http://dx.doi.org/10.1002/btpr.2781DOI Listing
January 2019

Determination of bisphenol A in red wine using a double vortex-ultrasound-assisted microextraction assay: Role of the interfacial properties.

Biotechnol Prog 2019 Jan 29:e2780. Epub 2019 Jan 29.

Dipartimento di Agricoltura, Ambiente Alimenti (DIAAA) and Consorzio Interuniversitario per lo Sviluppo dei Sistemi a Grande Interfase (CSGI), Università degli studi del Molise, Campobasso, Italy.

Bisphenol A (BPA) is a synthetic compound broadly used in medical devices as well as in packaging of food and drinks. Recently, BPA toxicity has become of concern to environmental public health. Red wine that is susceptible to BPA contamination is an alcoholic beverage made from yeast fermentation of grapes in the presence of grape skins so as to extract phenolic compounds. Read More

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http://dx.doi.org/10.1002/btpr.2780DOI Listing
January 2019
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Plasmid-encoded protein attenuates Escherichia coli swimming velocity and cell growth, not reprogrammed regulatory functions.

Biotechnol Prog 2019 Jan 21:e2778. Epub 2019 Jan 21.

Institute for Bioscience and Biotechnology Research, MD, 20742.

In addition to engineering new pathways for synthesis, synthetic biologists rewire cells to carry out "programmable" functions, an example being the creation of wound-healing probiotics. Engineering regulatory circuits and synthetic machinery, however, can be deleterious to cell function, particularly if the "metabolic burden" is significant. Here, a synthetic regulatory circuit previously constructed to direct Escherichia coli to swim toward hydrogen peroxide, a signal of wound generation, was shown to work even with coexpression of antibiotic resistance genes and genes associated with lactose utilization. Read More

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http://dx.doi.org/10.1002/btpr.2778DOI Listing
January 2019
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Investigation of fouling mechanisms of virus filters during the filtration of protein solutions using a high throughput filtration screening device.

Biotechnol Prog 2019 Jan 10:e2776. Epub 2019 Jan 10.

Sartorius Stedim Biotech GmbH, August-Spindler-Straße 11, 37079 Göttingen, Germany.

The downstream process development of novel antibodies (Abs) is often challenged by virus filter fouling making a better understanding of the underlying mechanisms highly desirable. The present study combines the protein characterization of different feedstreams with their virus filtration performance using a novel high throughput filtration screening system. Filtration experiments with Ab concentrations of up to 20 g/L using either low interacting or hydrophobically interacting pre-filters indicate the existence of two different fouling mechanisms, an irreversible and a reversible one. Read More

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January 2019

A comparison between continuous and batch processes to produce phosphatidylserine in the efficient and green aqueous-solid system.

Biotechnol Prog 2019 Jan 10. Epub 2019 Jan 10.

School of Chemical Engineering, Northwest University, 710069, Xi'an, Shaanxi, China.

The efficient and environmentally friendly aqueous-solid systems employed Triton-X-100-modified silica as the "artificial interface" to adsorb phosphatidylcholine (PC) in purely aqueous solutions and silica-adsorbed PC was successfully used for phospholipase D (PLD)-mediated transphosphatidylation. Three kinds of silicas with different sizes were employed to investigate advantages and disadvantages of batch and continuous technologies for PLD-catalyzed transphosphatidylation in aqueous-solid systems. The highest yields of product were obtained in the batch technology, but the continuous production had the simplest operational process and highest space-time yield. Read More

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http://doi.wiley.com/10.1002/btpr.2777
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http://dx.doi.org/10.1002/btpr.2777DOI Listing
January 2019
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Pilot-scale process for magnetic bead purification of antibodies directly from non-clarified CHO cell culture.

Biotechnol Prog 2019 Jan 10. Epub 2019 Jan 10.

AdBIOPRO, VINNOVA Competence Centre for Advanced BioProduction by Continuous Processing, Stockholm, Sweden.

High capacity magnetic protein A agarose beads, LOABeads PrtA, were used in the development of a new process for affinity purification of monoclonal antibodies (mAbs) from non-clarified CHO cell broth using a pilot-scale magnetic separator. The LOABeads had a maximum binding capacity of 65 mg/mL and an adsorption capacity of 25-42 mg IgG/mL bead in suspension for an IgG concentration of 1 to 8 g/L. Pilot-scale separation was initially tested in a mAb capture step from 26 L clarified harvest. Read More

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http://dx.doi.org/10.1002/btpr.2775DOI Listing
January 2019

Optimization and scale up of itaconic acid production from potato starch waste in stirred tank bioreactor.

Biotechnol Prog 2019 Jan 9. Epub 2019 Jan 9.

Academy of Scientific and Innovative Research, CSIR-National Environmental Engineering Research Institute, Nagpur, 440020, India.

Present study used Aspergillus terreus strain C1 isolated from mangrove soil for itaconic acid (IA) production from potato starch waste. Fermentation parameters were optimized by classical one factor approach and statistical experimental designs, such as Plackett-Burman and response surface designs. Anionic deionization of potato waste was found to be a very effective, economic, and easy way of improving IA production. Read More

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http://dx.doi.org/10.1002/btpr.2774DOI Listing
January 2019

Genetic and in silico analysis of plantaricin EFI locus in indigenous isolates of lactobacillus plantarum.

Biotechnol Prog 2019 Mar 22;35(2):e2773. Epub 2019 Jan 22.

Dept. of Food Biotechnology, Branch for Northwest & West region, Agricultural Biotechnology Research Inst. of Iran, Agricultural Research, Education and Extension Organization (AREEO), Tabriz, Iran.

Genetic investigation and in silico analysis of plantaricin EFI (plnEFI) locus was performed in three indigenous isolates of Lactobacillus plantarum EL3, L28 and BL1. Amplification with plnEFI specific primers and production of ~ 10 KDa size protein suggested the existence of class II bacteriocins. The analysis demonstrated that the studied fragment included structural bacteriocin, immunity, partial transporter and potential regulatory encoding regions. Read More

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http://dx.doi.org/10.1002/btpr.2773DOI Listing

Utilizing a regulated targeted integration cell line development approach to systematically investigate what makes an antibody difficult to express.

Biotechnol Prog 2019 Mar 10;35(2):e2772. Epub 2019 Jan 10.

Dept. of Cell Culture, Genentech Inc., South San Francisco, California, 94080.

Chinese hamster ovary (CHO) cells are conventionally used to generate therapeutic cell lines via random integration (RI), where desired transgenes are stably integrated into the genome. Targeted integration (TI) approaches, which involve integration of a transgene into a specific locus in the genome, are increasingly utilized for CHO cell line development (CLD) in recent years. None of these CLD approaches, however, are suitable for expression of toxic or difficult-to-express molecules, or for determining the underlying causes for poor expression of some molecules. Read More

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http://dx.doi.org/10.1002/btpr.2772DOI Listing
March 2019
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Nutrient modulation based process engineering strategy for improved butanol production from Clostridium acetobutylicum.

Biotechnol Prog 2019 Mar 14;35(2):e2771. Epub 2019 Jan 14.

Dept. of Biosciences & Bioengineering, Indian Inst. of Technology, Guwahati, Assam, 781039, India.

The present study demonstrates a process engineering strategy to achieve high butanol titer and productivity from wild type Clostridium acetobutylicum MTCC 11274. In the first step, two different media were optimized with the objectives of maximizing the biomass and butanol productivity, respectively. In the next step, attributes of these two media compositions were integrated to design a two-stage fed-batch process which resulted in maximal butanol productivity of 0. Read More

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http://dx.doi.org/10.1002/btpr.2771DOI Listing
March 2019
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