4,882 results match your criteria Biotechnology progress[Journal]


Purification of Rabies Virus Glycoprotein produced in Drosophila melanogaster S2 cells: an efficient immunoaffinity method.

Biotechnol Prog 2020 Jul 6:e3046. Epub 2020 Jul 6.

Science and Technology Institute, Federal University of São Paulo (UNIFESP), São José dos Campos, Brazil.

Most rabies vaccines are based on inactivated virus, which production process demands a high level of biosafety structures. In the past decades recombinant Rabies Virus Glycoprotein (RVGP) produced in several expression systems, has been extensively studied to be used as an alternative vaccine. The immunogenic characteristics of this protein depend on its correct conformation, which is present only after the correct post-translational modifications, typically performed by animal cells. Read More

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http://dx.doi.org/10.1002/btpr.3046DOI Listing

Glycan Engineering Reveals Interrelated Effects of Terminal Galactose and Core Fucose on Antibody-dependent Cell-mediated Cytotoxicity.

Biotechnol Prog 2020 Jul 5:e3045. Epub 2020 Jul 5.

Attribute Sciences, Amgen Inc., Thousand Oaks, CA.

Antibody-dependent cell-mediated cytotoxicity (ADCC) has been identified as one of the potentially critical effector functions underlying the clinical efficacy of some therapeutic IgG1 antibodies. It has been well established that higher levels of afucosylated N-linked glycan structures on the Fc region enhance the IgG binding affinity to the FcγIIIa receptor and lead to increased ADCC activity. However, whether terminal galactosylation of an IgG1 impacts its ADCC activity is less understood. Read More

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http://dx.doi.org/10.1002/btpr.3045DOI Listing

Development of a Small-Scale Rotary Lobe Pump Cell Culture Model for Examining Cell Damage in Large-Scale N-1 Seed Perfusion Process.

Biotechnol Prog 2020 Jun 28:e3044. Epub 2020 Jun 28.

Manufacturing Sciences, Biogen, Research Triangle Park, North Carolina.

Perfusion technology has been identified as a process improvement capable of eliminating some of the constraints in cell culture and allows for high cell densities and viabilities. However, when implementing this N-1 seed perfusion platform in large-scale manufacturing, unexpected cell damage was observed as early as day one. Given shear rate within recirculation hollow fibers was normalized and aligned correctly across bench, pilot and manufacture scale, the primary mitigation was placed on the rotary lobe pump. Read More

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http://dx.doi.org/10.1002/btpr.3044DOI Listing

Decoration of electrical conductive polyurethane-polyaniline/polyvinyl alcohol matrixes with mussel-inspired polydopamine for bone tissue engineering.

Biotechnol Prog 2020 Jun 26. Epub 2020 Jun 26.

Department of Orthopedics, Shanghai Pudong Hospital, Fudan University Pudong Medical Center, 2800 Gongwei Road, Pudong, Shanghai, China.

Electrospinning is a versatile technology for the fabrication of nanofibrous matrixes to regenerate defects. This study aims to develop a functionalized and electro-conductive polymeric matrix to improve rat bone marrow mesenchymal stem cell adhesion, proliferation, and differentiation. Herein, the influence of the chemical composition of the substrate on homogeneous modification of the surface with mussel-inspired polydopamine (PDA) is focused. Read More

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http://dx.doi.org/10.1002/btpr.3043DOI Listing

Traces matter: targeted optimization of mAb N-glycosylation based on/by implementing automated high-throughput trace element screening.

Biotechnol Prog 2020 Jun 25:e3042. Epub 2020 Jun 25.

Roche Diagnostics GmbH, Cell Culture Research, Pharma Research and Early Development, Roche Innovation Center Munich, pRED, LMR, Penzberg, Germany.

The use of high-throughput systems in cell culture process optimization offers various opportunities in biopharmaceutical process development. Here we describe the potential for acceleration and enhancement of product quality optimization and de novo bioprocess design regarding monoclonal antibody N-glycosylation by using an iterative statistical Design of Experiments (DoE) strategy based on our automated microtiter plate (MTP)-based system for suspension cell culture. In our example, the combination of an initial screening of trace metal building blocks with a comprehensive DoE-based screening of 13 different trace elemental ions at three concentration levels in one run revealed most effective levers for N-glycan processing and biomass formation. Read More

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http://dx.doi.org/10.1002/btpr.3042DOI Listing

High Throughput Solubility and Re-dissolution Screening for Antibody Purification via Combined PEG and Zinc Chloride Precipitation.

Biotechnol Prog 2020 Jun 24:e3041. Epub 2020 Jun 24.

Department of Chemical Engineering, The Pennsylvania State University, State College PA 16801.

As upstream product titers increase, the downstream chromatographic capture step has become a significant "downstream bottleneck". Precipitation appears to be more attractive under these conditions as the supersaturation driving force increases with the ever-increasing titer. In this study, two precipitating reagents with orthogonal mechanisms, polyethylene glycol (PEG) as a volume excluder and zinc chloride (ZnCl ) as a cross linker, were examined as precipitants for two monoclonal antibodies (mAbs), one was stable and the other was aggregation-prone, in purified drug substance and harvested cell culture fluid forms. Read More

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http://dx.doi.org/10.1002/btpr.3041DOI Listing

Modulation of transmembrane pressure in manufacturing scale tangential flow filtration N-1 perfusion seed culture.

Biotechnol Prog 2020 Jun 24:e3040. Epub 2020 Jun 24.

Process Sciences, Global Manufacturing Sciences, Biogen, Research Triangle Park, Durham, North Carolina, USA.

Mammalian cells were grown to high density in a 3,000 L culture using perfusion with hollow fibers operated in a tangential flow filtration mode. The high-density culture was used to inoculate the production stage of a biomanufacturing process. At constant permeate flux operation, increased transmembrane pressures (TMPs) were observed on the final day of the manufacturing batches. Read More

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http://dx.doi.org/10.1002/btpr.3040DOI Listing

Nanoelicitor based enhancement of camptothecin production in fungi isolated from Ophiorrhiza mungos.

Biotechnol Prog 2020 Jun 18:e3039. Epub 2020 Jun 18.

School of Biosciences, Mahatma Gandhi University, Kottayam, India.

In the study, endophytic fungi isolated from Ophiorrhiza mungos were screened for camptothecin (CPT) biosynthetic potential by high performance liquid chromatography (HPLC). Among the 16 fungi screened, OmF3, OmF4, and OmF6 were identified to synthesize CPT. Further LC-MS analysis also showed the presence of CPT specific m/z of 349 for the extracts from OmF3, OmF4, and OmF6. Read More

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http://dx.doi.org/10.1002/btpr.3039DOI Listing

Osmolality as a lever to modulate the N-glycolylneuraminic acid (Neu5Gc) level of a recombinant glycoprotein produced in Chinese hamster ovary cells.

Biotechnol Prog 2020 Jun 15. Epub 2020 Jun 15.

Manufacturing Science & Technology, Bristol Myers Squibb Co., 38 Jackson Road, Devens, Massachusetts.

Glycoproteins could be highly sialylated and controlling the sialic acid levels for some therapeutic proteins is critical to ensure product consistency and efficacy. N-acetylneuraminic acid (Neu5Ac, or NANA) and N-glycolylneuraminic acid (Neu5Gc, or NGNA) are two most common forms of sialic acids produced in mammalian cells. As Neu5Gc is not produced in humans and can elicit immune responses, minimizing Neu5Gc formation is important in controlling this quality attribute for complex glycoproteins. Read More

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http://dx.doi.org/10.1002/btpr.3038DOI Listing

Identification and characterization of a Triton X-100 replacement for virus inactivation.

Biotechnol Prog 2020 Jun 12:e3036. Epub 2020 Jun 12.

Bioproduct Research and Development, Lilly Research Laboratories, Eli Lilly and Company, Lilly Corporate Center, Indianapolis, Indiana, USA.

Triton X-100 detergent treatment is a robust enveloped virus inactivation unit operation included in biopharmaceutical manufacturing processes. However, the European Commission officially placed Triton X-100 on the Annex XIV authorization list in 2017 because a degradation product of Triton X-100, 4-(1,1,3,3-tetramethylbutyl) phenol (also known as 4-tert-octylphenol), is considered to have harmful endocrine disrupting activities. As a result, the use of Triton X-100 in the European Economic Area (EEA) would not be allowed unless an ECHA issued authorization was granted after the sunset date of January 4, 2021. Read More

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http://dx.doi.org/10.1002/btpr.3036DOI Listing

Simultaneous and rapid quantification of microalga biomolecule content using electrochemical impedance spectroscopy.

Biotechnol Prog 2020 Jun 12:e3037. Epub 2020 Jun 12.

Department of Chemical and Petroleum Engineering, College of Engineering, UAE University, United Arab Emirates.

Lipids, proteins, and carbohydrates are the major constituents found in microalga cells, in varying proportions, and these biomolecules find applications in different industries. During microalga cultivation, to efficiently manipulate, control, and optimize the productivity of a specific compound for a specific application, real-time monitoring of these three cell components is essential. In this study, a method using measurement of electrical capacitance was developed to simultaneously determine the lipid, protein, and carbohydrate content of microalga cells without the requirement for any pre-processing steps. Read More

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http://dx.doi.org/10.1002/btpr.3037DOI Listing

The Effect of Magnetized Water on the Oxidation Reaction of Phenol Derivatives and Aromatic Amines by Horseradish Peroxidase Enzyme.

Biotechnol Prog 2020 Jun 9:e3035. Epub 2020 Jun 9.

Department of Chemistry, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran.

The present study aimed to investigate, for the first time, the rate of the oxidation reaction of some derivatives of phenol and aromatic amines, that is, Pyrogallol (PGL), Catechol (CTL), Resorcinol (RSL), Ortho-Aminophenol (OAP), Meta-Aminophenol (MAP), Para-Aminophenol (PAP), Ortho-Phenylenediamine (OPD) and Para-Phenylenediamine (PPD), in the presence of hydrogen peroxide in pure and magnetized solvents using horseradish peroxidase enzyme. The reaction was studied in the absence and presence of a magnetized solvent under completely identical conditions. The results showed that magnetized solvent could change the structure of the enzyme and reduce its activity. Read More

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http://dx.doi.org/10.1002/btpr.3035DOI Listing

Establishment and Characterization of an In Vitro 3D Ovarian Cancer Model for Drug Screening Assays.

Biotechnol Prog 2020 Jun 9:e3034. Epub 2020 Jun 9.

School of Pharmaceutical Sciences of Ribeirao Preto, University of Sao Paulo, Ribeirão Preto, SP, Brazil.

The acquired drug chemoresistance represents the main challenge of the ovarian cancer treatment. In addition, the absence of an adequate in vitro model able to reproduce the native tumor environment can contribute to the poor success rate of pre-clinical studies of new compounds. Three-dimensional (3D) culture models have been recently used for drug screening purposes due to their ability to reproduce the main characteristics of in vivo solid tumors. Read More

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http://dx.doi.org/10.1002/btpr.3034DOI Listing

Stabilization of immobilized l-arabinose isomerase for the production of d-tagatose from d-galactose.

Biotechnol Prog 2020 Jun 7:e3033. Epub 2020 Jun 7.

Department for Innovation in Biological, Agro-food and Forest Systems, University of Tuscia, Viterbo, Italy.

The aim of this work was to develop a stable immobilized enzyme biocatalyst for the isomerization of d-galactose to d-tagatose at high temperature. l-Arabinose isomerase from the hyperthermophilic bacterium Thermotoga maritima (TMAI) was produced as a (His) -tagged protein, immobilized on a copper-chelate epoxy support and subjected to several postimmobilization treatments aimed at increasing its operational and structural stability. Treatment with glutaraldehyde and ethylenediamine resulted in a more than twofold increase in the operational stability and in all enzyme subunits linked, directly or indirectly, to the support via covalent bonds. Read More

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http://dx.doi.org/10.1002/btpr.3033DOI Listing

Bioremediation of water contaminated with uranium using Penicillium piscarium.

Biotechnol Prog 2020 May 31:e30322. Epub 2020 May 31.

Laboratório de Micotoxinas, Departamento de Microbiologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, Brazil.

Penicillium piscarium can be indicated as promising in the treatment of sites contaminated with uranium. Thus, this research aimed to analyze the P. piscarium dead biomass in uranium biosorption. Read More

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http://dx.doi.org/10.1002/btpr.3032DOI Listing

Biosurfactants: Production and application prospects in the food industry.

Biotechnol Prog 2020 May 28:e3030. Epub 2020 May 28.

Centre for Science and Technology, Catholic University of Pernambuco, Recife, Brazil.

There has been considerable interest in the use of biosurfactants due to the diversity of structures and the possibility of production from a variety of substrates. The potential for industrial applications has been growing, as these natural compounds are tolerant to common processing methods and can compete with synthetic surfactants with regards to the capacity to reduce surface and interfacial tensions as well as stabilise emulsions while offering the advantages of biodegradability and low toxicity. Among biosurfactant-producing microorganisms, some yeasts present no risks of toxicity or pathogenicity, making them ideal for use in food formulations. Read More

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http://dx.doi.org/10.1002/btpr.3030DOI Listing

A suitable and effective stepwise oxidative refolding procedure for highly-cationic tetrameric avidin in nucleic acid free conditions.

Biotechnol Prog 2020 May 28:e3031. Epub 2020 May 28.

Department of Interdisciplinary Science and Engineering in Health Systems, Okayama University, Okayama, Japan.

Optimized conditions are needed to refold recombinant proteins from bacterial inclusion bodies into their biologically active conformations. In this study, we found two crucial requirements for efficient refolding of cationic tetrameric chicken avidin. The first step is to eliminate nucleic acid contaminants from the bacterial inclusion body. Read More

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http://dx.doi.org/10.1002/btpr.3031DOI Listing

Cadmium binding characterization and mechanism of a newly isolated strain Cystobasidium oligophagum QN-3.

Authors:
Wen Li Tao Wang

Biotechnol Prog 2020 May 28:e3029. Epub 2020 May 28.

Jiangsu Key Construction Laboratory of Food Resource Development and Quality Safe, Xuzhou University of Technology, Xuzhou, PR China.

The aim of this study was to screen a strain for the removal of Cd from aqueous solution and investigate the characterization and mechanism of the Cd binding process. A novel strain of yeast showed high tolerance of cadmium, namely Cystobasidium oligophagum QN-3, was isolated from soils, which could resist 22,000 mg/L and 18,000 mg/L Cd on PDA (potato dextrose agar) plate and in PDA liquid medium, respectively. Cd binding experiment showed that the strain could remove Cd from aqueous solution effectively, the maximum Cd removal rate of 84. Read More

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http://dx.doi.org/10.1002/btpr.3029DOI Listing

Effectiveness of host cell protein removal using depth filtration with a filter containing diatomaceous earth.

Biotechnol Prog 2020 May 23:e3028. Epub 2020 May 23.

Department of Chemical Engineering, The Pennsylvania State University, University Park, Pennsylvania, USA.

The increased cell density and product titer in biomanufacturing have led to greater use of depth filtration as part of the initial clarification of cell culture fluid, either as a stand-alone unit operation or after centrifugation. Several recent studies have shown that depth filters can also reduce the concentration of smaller impurities like host cell proteins (HCP) and DNA, decreasing the burden on subsequent chromatographic operations. The objective of this study was to evaluate the HCP removal properties of the Pall PDH4 depth filter media, a model depth filter containing diatomaceous earth, cellulose fibers, and a binder. Read More

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http://dx.doi.org/10.1002/btpr.3028DOI Listing

Effects of different graphene-based nanomaterials as elicitors on growth and ganoderic acid production by Ganoderma lucidum.

Biotechnol Prog 2020 May 20:e3027. Epub 2020 May 20.

Department of Microbiology, Faculty of Medicine, AJA University of Medical Sciences, Tehran, Iran.

Graphene-based nanomaterials (GBNs) have attracted considerable interest nowadays due to their wide range of applications. However, very little attention has been paid to the application of nanomaterials as potential elicitors for production of valuable metabolites. Herein, aiming to earn insight into effects of nanomaterials on secondary metabolite biosynthesis by medicinal fungi, we evaluated the influence of GBNs on growth and production of ganoderic acid (GA) by Ganoderma lucidum in submerged culture. Read More

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http://dx.doi.org/10.1002/btpr.3027DOI Listing

Reduction of medium consumption in perfusion mammalian cell cultures using a perfusion rate equivalent concentrated nutrient feed.

Biotechnol Prog 2020 May 16:e3026. Epub 2020 May 16.

Biotech Process Sciences, Merck Biopharma, Corsier-sur-Vevey, Switzerland.

Media preparation for perfusion cell culture processes contributes significantly to operational costs and the footprint of continuous operations for therapeutic protein manufacturing. In this study, definitions are given for the use of a perfusion equivalent nutrient feed stream which, when used in combination with basal perfusion medium, supplements the culture with targeted compounds and increases the medium depth. Definitions to compare medium and feed depth are given in this article. Read More

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http://dx.doi.org/10.1002/btpr.3026DOI Listing

Integration of ion exchange resin materials for a downstream-processing approach of an imine reductase-catalyzed reaction.

Biotechnol Prog 2020 May 14:e3024. Epub 2020 May 14.

Biocatalytic Synthesis Group, Institute of Chemistry, University of Rostock, Rostock, Germany.

In this study, an ion exchange resin-based downstream-processing concept for imine reductase (IRED)-catalyzed reactions was investigated. As a model reaction, 2-methylpyrroline was converted to its corresponding product (S)-2-methylpyrrolidine with >99% of conversion by the (S)-selective IRED from Paenibacillus elgii B69. Under optimized reaction conditions full conversion was achieved using a substrate concentration of 150 and 500 mmol/L of d-glucose. Read More

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http://dx.doi.org/10.1002/btpr.3024DOI Listing

Mesenchymal stem cells engineered by modified polyethylenimine polymer for targeted cancer gene therapy, in vitro and in vivo.

Biotechnol Prog 2020 May 14:e3025. Epub 2020 May 14.

Pharmaceutical Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran.

Cell-based delivery system is a promising strategy to protect therapeutic agents from the immune system and provide targeted delivery. Mesenchymal stem cells (MSCs) have recently been introduced as an encouraging vehicle in cell-based gene therapy due to their unique features including tumor-tropic property and migratory ability. However, gene transfer into MSCs is limited due to low efficiency and cytotoxicity of carriers. Read More

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http://dx.doi.org/10.1002/btpr.3025DOI Listing

Erratum.

Authors:

Biotechnol Prog 2020 May 12:e3005. Epub 2020 May 12.

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http://dx.doi.org/10.1002/btpr.3005DOI Listing

Optimization of culture media to enhance the growth of tissue engineered cartilage.

Biotechnol Prog 2020 May 11:e3017. Epub 2020 May 11.

Biomedical Engineering, Ryerson University, Toronto, Ontario, Canada.

Tissue engineering is a promising option for cartilage repair. However, several hurdles still need to be overcome to develop functional tissue constructs suitable for implantation. One of the most common challenges is the general low capacity of chondrocytes to synthesize cartilage-specific extracellular matrix (ECM). Read More

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http://dx.doi.org/10.1002/btpr.3017DOI Listing

Highly sensitive HPLC analysis and biophysical characterization of N-glycans of IgG-Fc domain in comparison between CHO and 293 cells using FcγRIIIa ligand.

Biotechnol Prog 2020 May 11:e3016. Epub 2020 May 11.

School of Engineering, The University of Tokyo, Tokyo, Japan.

Quality control of monoclonal antibodies is challenging due in part to the diversity of post-translational modifications present. The regulation of the N-glycans of IgG-Fc domain is one of the key factors to maintain the safety and efficacy of antibody drugs. The FcγRIIIa affinity column is an attractive tool for the precise analysis of the N-glycans in IgG-Fc domain. Read More

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http://dx.doi.org/10.1002/btpr.3016DOI Listing

Evaluation of quenching methods for metabolite recovery in photoautotrophic Synechococcus sp. PCC 7002.

Biotechnol Prog 2020 May 10:e3015. Epub 2020 May 10.

Chemical & Biological Engineering , Colorado School of Mines, Golden, Colorado, USA.

The first step of many metabolomics studies is quenching, a technique vital for rapidly halting metabolism and ensuring that the metabolite profile remains unchanging during sample processing. The most widely used approach is to plunge the sample into prechilled cold methanol; however, this led to significant metabolite loss in Synecheococcus sp. PCC 7002. Read More

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http://dx.doi.org/10.1002/btpr.3015DOI Listing

Newly synthesized peptide, Ara-27, exhibits significant improvement in cell-penetrating ability compared to conventional peptides.

Biotechnol Prog 2020 May 6:e3014. Epub 2020 May 6.

Cellular Reprogramming and Embryo Biotechnology Laboratory, Dental Research Institute, School of Dentistry, Seoul National University, Seoul, Korea.

Cell-penetrating peptides (CPPs) are short amino acid sequences known to act as a vehicle for enhancing the intracellular translocating efficiency of extracellular molecules. Although many groups have attempted to develop peptides with high cell-penetrating efficiencies, very few have demonstrated efficient cellular uptake of CPPs at low concentrations. Here, we describe a newly synthesized peptide derived from Arabidopsis, Ara-27, which exhibits significant improvement in cell-penetrating efficiency compared to existing CPPs. Read More

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http://dx.doi.org/10.1002/btpr.3014DOI Listing

Characterization of liver zonation-like transcriptomic patterns in HLCs derived from hiPSCs in a microfluidic biochip environment.

Biotechnol Prog 2020 May 4:e3013. Epub 2020 May 4.

CNRS UMI 2820, Laboratory for Integrated Micro Mechatronic Systems, Institute of Industrial Science, University of Tokyo, Tokyo, Japan.

The liver zonation is an important phenomenon characterized by a gradient of several functions along the liver acinus. However, this gradient remains difficult to reproduce in in-vitro conditions, making the obtention of an in-vitro method to recapitulate the liver zonation a challenging issue. In this study, we evaluated the spatial evolution of the transcriptome profile of human induced pluripotent stem cells (hiPSCs) differentiated toward hepatocytes-like cells (HLCs) phenotype in a microfluidic biochip environment. Read More

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http://dx.doi.org/10.1002/btpr.3013DOI Listing

Cell culture process metabolomics together with multivariate data analysis tools opens new routes for bioprocess development and glycosylation prediction.

Biotechnol Prog 2020 May 4:e3012. Epub 2020 May 4.

Department of Chemistry and Applied Biosciences, Institute of Chemical and Bioengineering, ETH Zürich, Switzerland.

Multivariate latent variable methods have become a popular and versatile toolset to analyze bioprocess data in industry and academia. This work spans such applications from the evaluation of the role of the standard process variables and metabolites to the metabolomics level, that is, to the extensive number metabolic compounds detectable in the extracellular and intracellular domains. Given the substantial effort currently required for the measurement of the latter groups, a tailored methodology is presented that is capable of providing valuable process insights as well as predicting the glycosylation profile based on only four experiments measured over 12 cell culture days. Read More

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http://dx.doi.org/10.1002/btpr.3012DOI Listing

Biosynthesis of vitamin B12 by Propionibacterium freudenreichii subsp. shermanii ATCC 13673 using liquid acid protein residue of soybean as culture medium.

Biotechnol Prog 2020 May 1:e3011. Epub 2020 May 1.

Biotechnology & Biochemical Engineering Laboratory (BiotecLab), Food Science and Technology Institute, Federal University of Rio Grande do Sul, Porto Alegre, Brazil.

Vitamin B12 deficiency still persists, mainly caused by low intake of animal food products affecting vegetarians, vegans, and populations of underdeveloped countries. In this study, we investigate the biosynthesis of vitamin B12 by potential probiotic bacterium using an agroindustry residue, the liquid acid protein residue of soybean (LAPRS), as a low-cost, animal derivate-free alternative culture medium. Cultures of Propionibacterium freudenreichii subsp. Read More

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http://dx.doi.org/10.1002/btpr.3011DOI Listing

Tangential flow filtration of haptoglobin.

Biotechnol Prog 2020 Apr 29:e3010. Epub 2020 Apr 29.

William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, Columbus, Ohio, USA.

Haptoglobin (Hp) is a plasma glycoprotein that scavenges cell-free hemoglobin (Hb). Hp has various potential therapeutic applications, but it has been mainly studied for treatment of acute hemolytic conditions that can arise from situations such as massive blood transfusion, infusion of stored red blood cells, severe burns, trauma, sepsis, radiation injury, and others. Therefore, Hp may also be beneficial during chronic hemolytic disease states such as hereditary spherocytosis, nocturnal hemoglobinuria, sickle-cell anemia, and malaria. Read More

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http://dx.doi.org/10.1002/btpr.3010DOI Listing

Molecularly imprinted cryogel cartridges for the selective recognition of tyrosine.

Biotechnol Prog 2020 Apr 23:e3006. Epub 2020 Apr 23.

Department of Chemistry, Hacettepe University, Ankara, Turkey.

Molecularly imprinted polymers are used for creating a specific cavity and selective recognition sites for the structure of a target molecule in a polymeric structure. In this study, specific molecularly imprinted cryogel cartridges were synthesized using two distinct functional monomers to compare imprinting efficiency for the selective recognition of Tyrosine (Tyr). Tyr-imprinted cryogel cartridge (MIP1) was prepared using metal-chelate coordination for the imprinting process by free-radical bulk polymerization under frozen conditions, and Tyr-imprinted cryogel cartridge (MIP2) was prepared in the same way using hydrophobic effects for imprinting. Read More

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http://dx.doi.org/10.1002/btpr.3006DOI Listing

Wireless bioreactor for anaerobic cultivation of bacteria.

Biotechnol Prog 2020 Apr 23:e3009. Epub 2020 Apr 23.

Department of Electrical Engineering, National Tsing Hua University, Hsinchu, Taiwan.

Anaerobic cultivation methods of bacteria are indispensable in microbiology. One methodology is to cultivate the microbes in anaerobic enclosure with oxygen-adosrbing chemicals. Here, we report an electronic extension of such strategy for facultative anaerobic bacteria. Read More

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http://dx.doi.org/10.1002/btpr.3009DOI Listing

A simplified method for the efficient purification and refolding of recombinant human TRAIL.

Biotechnol Prog 2020 Apr 23:e3007. Epub 2020 Apr 23.

Department of Biomedical Engineering, Vanderbilt University, Nashville, TN, USA.

Tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) belongs to the TNF cytokine superfamily that specifically induces apoptosis in a broad spectrum of human cancer cell lines but not in most healthy cells. The antitumor potential of recombinant human TRAIL (rhTRAIL) has attracted great attention among biologists and oncologists. However, attempts to express rhTRAIL in Escherichia coli often results in limited yield of bioactive protein due to the formation of inclusion bodies (IBs), which are dense insoluble particulate protein aggregates inside cells. Read More

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http://dx.doi.org/10.1002/btpr.3007DOI Listing

Recent advances in domesticating non-model microorganisms.

Biotechnol Prog 2020 Apr 23:e3008. Epub 2020 Apr 23.

Department of Chemical and Biomolecular Engineering, Carl R. Woese Institute for Genomic Biology , University of Illinois at Urbana-Champaign, Urbana, Illinois, USA.

Non-model microorganisms have been increasingly explored as microbial cell factories for production of chemicals, fuels, and materials owing to their unique physiology and metabolic capabilities. However, these microorganisms often lack facile genetic tools for strain development, which hinders their adoption as production hosts. In this review, we describe recent advances in domestication of non-model microorganisms, including bacteria, actinobacteria, cyanobacteria, yeast, and fungi, with a focus on the development of genetic tools. Read More

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http://dx.doi.org/10.1002/btpr.3008DOI Listing

Elemental metal variance in cell culture raw materials for process risk profiling.

Biotechnol Prog 2020 Apr 20:e3004. Epub 2020 Apr 20.

Materials Science, Durham, North Carolina, USA.

Elemental metals are critical raw material attributes which can impact cell culture performance and associated therapeutic protein product quality profiles. Metals such as copper and manganese act as cofactors and reagents for numerous metabolic pathways which govern cell growth, protein expression, and glycosylation, thus mandating elemental monitoring. The growing complexity of modern cell culture media formulations adds additional opportunities for elemental variance and its associated impact risks. Read More

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http://dx.doi.org/10.1002/btpr.3004DOI Listing

Development of a scale-up strategy for Chinese hamster ovary cell culture processes using the k a ratio as a direct indicator of gas stripping conditions.

Biotechnol Prog 2020 Apr 16:e3000. Epub 2020 Apr 16.

Institute of Bioscience and Bioindustry, Tokushima University, Tokushima, Japan.

Herein, we described a scale-up strategy focused on the dissolved carbon dioxide concentration (dCO ) during fed-batch cultivation of Chinese hamster ovary cells. A fed-batch culture process for a 2000-L scale stainless steel (SS) bioreactor was scaled-up from similarly shaped 200-L scale bioreactors based on power input per unit volume (P/V). However, during the 2000-L fed-batch culture, the dCO was higher compared with the 200-L scale bioreactor. Read More

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http://dx.doi.org/10.1002/btpr.3000DOI Listing

Purification of Pseudomonas sp. proteases through aqueous biphasic systems as an alternative source to obtain bioactive protein hydrolysates.

Biotechnol Prog 2020 Apr 12:e3003. Epub 2020 Apr 12.

Laboratorio de Biología Molecular, Facultad de Farmacia y Bioquímica, Universidad Nacional Mayor de San Marcos, Lima, Peru.

Aqueous biphasic systems (ABSs) are an interesting alternative for separating industrial enzymes due to easy scale-up and low operational cost. The proteases of Pseudomonas sp. M211 were purified through ABS platforms formed by polyethylene glycol (PEG) and citrate buffer salt. Read More

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http://dx.doi.org/10.1002/btpr.3003DOI Listing

Targeted double domain nanoplex based on galactosylated polyethylenimine enhanced the delivery of IL-12 plasmid.

Biotechnol Prog 2020 Apr 12:e3002. Epub 2020 Apr 12.

Pharmaceutics Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran.

The objective of the present investigation was to design a targeted polyethylenimine (PEI)-based polyplex by conjugating lactose bearing galactose groups on low molecular weight PEI (LMW PEI) grafted to a high molecular weight PEI (HMW PEI) via a succinic acid linker in order to restore the amine content of the whole conjugate used for ligand conjugation. The PEI conjugate was synthesized and characterized in terms of buffering capacity, particle size, zeta potential, plasmid condensation ability, and protection of DNA against degrading enzymes. Also, the transfection efficiency and cytotoxicity were evaluated in the cell line over-expressing asialoglycoprotein receptors (ASGPRs) and compared with the cells lacking the receptors. Read More

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http://dx.doi.org/10.1002/btpr.3002DOI Listing

The effect of amino acid supplementation in an industrial Chinese Hamster Ovary process.

Biotechnol Prog 2020 Apr 10:e3001. Epub 2020 Apr 10.

Bioprocess Engineering, Integrated Bioprocess Development, TU Wien, Vienna, Austria.

The main goal in biosimilar development is to increase Chinese Hamster Ovary (CHO) viability and productivity while maintaining product quality. Despite media and feed optimization during process development, depletion of amino acids still occurs. The aim of the work was to optimize an existing industrial fed batch process by preventing shortage of amino acids and to gather knowledge about CHO metabolism. Read More

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http://dx.doi.org/10.1002/btpr.3001DOI Listing

Erratum.

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Biotechnol Prog 2020 Apr 8:e2997. Epub 2020 Apr 8.

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http://dx.doi.org/10.1002/btpr.2997DOI Listing

Extraction of recombinant periplasmic proteins under industrially relevant process conditions: Selectivity and yield strongly depend on protein titer and methodology.

Biotechnol Prog 2020 Apr 7:e2999. Epub 2020 Apr 7.

Christian Doppler Laboratory for Production of Next-Level Biopharmaceuticals in E. coli, Department of Biotechnology, University of Natural Resources and Life Sciences, Vienna, Austria.

In this work, we attempted to identify a method for the selective extraction of periplasmic endogenously expressed proteins, which is applicable at an industrial scale. For this purpose, we used an expression model that allows coexpression of two fluorescent proteins, each of which is specifically targeted to either the cytoplasm or periplasm. We assessed a number of scalable lysis methods (high-pressure homogenization, osmotic shock procedures, extraction with ethylenediaminetetraacetic acid, and extraction with deoxycholate) for the ability to selectively extract periplasmic proteins rather than cytoplasmic proteins. Read More

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http://dx.doi.org/10.1002/btpr.2999DOI Listing

Evaluation of exosome loading characteristics in their purification via a glycerol-assisted hydrophobic interaction chromatography method on a polyester, capillary-channeled polymer fiber phase.

Biotechnol Prog 2020 Apr 4:e2998. Epub 2020 Apr 4.

Department of Chemistry, Biosystems Research Complex, Clemson University, Clemson, South Carolina, USA.

Exosomes are membrane-secreted vesicles, with sizes ranging from 30 to 150 nm, which play key roles in intercellular communication. There is intense interest in developing methods to isolate and quantify exosomes toward clinical diagnostics, fundamental studies of intercellular processes, and use of exosomes as delivery vehicles for therapeutic agents. Current methods for exosomes isolation and quantification are time consuming and have operational high costs; few combine isolation and quantification into a singular operation unit. Read More

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http://dx.doi.org/10.1002/btpr.2998DOI Listing

Model-based design and control of a small-scale integrated continuous end-to-end mAb platform.

Biotechnol Prog 2020 Mar 31:e2995. Epub 2020 Mar 31.

Department of Industrial Biotechnology, School of Engineering Sciences in Chemistry, Biotechnology and Health, Royal Institute of Technology, Stockholm, Sweden.

A continuous integrated bioprocess available from the earliest stages of process development allows for an easier, more efficient and faster development and characterization of an integrated process as well as production of small-scale drug candidates. The process presented in this article is a proof-of-concept of a continuous end-to-end monoclonal antibody production platform at a very small scale based on a 200 ml alternating tangential flow filtration perfusion bioreactor, integrated with the purification process with a model-based design and control. The downstream process, consisting of a periodic twin-column protein A capture, a virus inactivation, a CEX column and an AEX column, was compactly implemented in a single chromatography system, with a purification time of less than 4 hr. Read More

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Pretreatments for enhancing clarification efficiency of depth filtration during production of monoclonal antibody therapeutics.

Biotechnol Prog 2020 Mar 29:e2996. Epub 2020 Mar 29.

Department of Chemical Engineering, Indian Institute of Technology, New Delhi, India.

High cell density, high product titer mammalian cell culture is the new paradigm for production of recombinant proteins. While the typical motivation is to get a high product titer, additional undesirable outcomes often include an increase in percentage solids in the cell culture fluid (cellular debris and sub-micron colloids), thereby offering new challenges to downstream processing. This article focuses on scouting and comparison of different approaches used for clarification of cell culture fluid. Read More

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http://dx.doi.org/10.1002/btpr.2996DOI Listing

Purification of animal immunoglobulin G (IgG) using peptoid affinity ligands.

Biotechnol Prog 2020 Mar 25:e2994. Epub 2020 Mar 25.

Department of Chemical and Biomolecular Engineering, North Carolina State University, Raleigh, North Carolina, USA.

The availability of highly pure animal antibodies is critical in the production of diagnostic tools and biosensors. The peptoid PL16, previously isolated from an ensemble of peptoid variants of the IgG-binding peptide HWRGWV, was utilized in this work as affinity ligand on WorkBeads resin for the purification of immunoglobulin G (IgG) from a variety of mammalian sources and chicken immunoglobulin Y (IgY). The chromatographic protocol initially optimized for murine serum and ascites was subsequently employed for processing rabbit, goat and sheep, donkey, llama, and chicken sera. Read More

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Preparation, statistical optimization and characterization of poly(3-hydroxybutyrate) fermented by Cupriavidus necator utilizing various hydrolysates of alligator weed (Alternanthera philoxeroides) as a sole carbon source.

Biotechnol Prog 2020 Mar 17:e2992. Epub 2020 Mar 17.

School of Food Science and Technology, Jiangnan University, Wuxi, China.

Alligator weed (Alternanthera philoxeroides) is a stoloniferous, amphibious and perennial herb which has invaded many parts of the world and led to serious environmental and ecological problems. In order to exploit cheap carbon source for poly(3-hydroxybutyrate) (PHB) production, alligator weed hydrolysates were prepared by acid and enzyme treatment and used for PHB production via Cupriavidus necator. The bacterium utilized alligator weed enzymatic hydrolysate and produced the PHB concentration of 3. Read More

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http://dx.doi.org/10.1002/btpr.2992DOI Listing

Mechanistic model of pH and excipient concentration during ultrafiltration and diafiltration processes of therapeutic antibodies.

Biotechnol Prog 2020 Mar 17:e2993. Epub 2020 Mar 17.

Process Engineering, Alcami Corporation, Wilmington, North Carolina, USA.

Ultrafiltration and diafiltration (UF/DF) unit operations are widely used for the manufacture of therapeutic antibodies to control drug substance protein concentration, pH, and excipient properties. During UF/DF, molecular interactions and volume exclusion effects often lead to substantial differences in pH and excipient concentrations between the diafiltration buffer and final UF/DF pool. These differences complicate the design process beyond simply specifying a buffer with the desired drug substance pH and excipient conditions. Read More

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http://dx.doi.org/10.1002/btpr.2993DOI Listing

Immobilization of horseradish peroxidase onto Purolite® A109 and its anthraquinone dye biodegradation and detoxification potential.

Biotechnol Prog 2020 Mar 13:e2991. Epub 2020 Mar 13.

Faculty of Technology and Metallurgy, University of Belgrade, Belgrade, Serbia.

Horseradish peroxidase (HRP) is a highly specific enzyme with great potential for use in the decolorization of synthetic dyes. A comprehensive study of HRP immobilization using various techniques such as adsorption and covalent immobilization on the novel carrier Purolite® A109 with a special focus on enzymatic decolorization and toxicity of artificially colored wastewater. The immobilized preparations with an activity of 156. Read More

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