1,552 results match your criteria Biotechnology for Biofuels [Journal]

Stochastic techno-economic analysis of the production of aviation biofuel from oilseeds.

Biotechnol Biofuels 2018 8;11:161. Epub 2018 Jun 8.

Institute for Future Environments, Science and Engineering Faculty, Queensland University of Technology (QUT), Brisbane, QLD Australia.

Background: The economic viability of hydrodeoxygenation process using Camelina, Carinata and Jatropha feedstocks for aviation biofuel production was evaluated for two product profiles: (i) maximum diesel production and (ii) maximum jet fuel production (HRJ).

Results: Deterministic analysis of Camelina and Carinata diesel facilities returned positive NPVs and IRRs of 25 and 18%, respectively. Stochastic analysis suggested that the probabilities of positive NPVs were 75, 59 and 15%, respectively, for Camelina, Carinata and Jatropha diesel plants. Read More

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A novel hybrid organosolv: steam explosion method for the efficient fractionation and pretreatment of birch biomass.

Biotechnol Biofuels 2018 8;11:160. Epub 2018 Jun 8.

1Biochemical Process Engineering, Division of Chemical Engineering, Department of Civil, Environmental, and Natural Resources Engineering, Luleå University of Technology, 971-87 Luleå, Sweden.

Background: The main role of pretreatment is to reduce the natural biomass recalcitrance and thus enhance saccharification yield. A further prerequisite for efficient utilization of all biomass components is their efficient fractionation into well-defined process streams. Currently available pretreatment methods only partially fulfill these criteria. Read More

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Insight into the functional roles of Glu175 in the hyperthermostable xylanase XYL10C-ΔN through structural analysis and site-saturation mutagenesis.

Biotechnol Biofuels 2018 8;11:159. Epub 2018 Jun 8.

1Key Laboratory for Feed Biotechnology of the Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing, 100081 China.

Background: Improving the hydrolytic performance of hemicellulases to degrade lignocellulosic biomass is of considerable importance for second-generation biorefinery. Xylanase, as the crucial hemicellulase, must be thermostable and have high activity for its potential use in the bioethanol industry. To obtain excellent xylanase candidates, it is necessary to understand the structure-function relationships to provide a meaningful reference to improve the enzyme properties. Read More

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June 2018
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Optimization of odd chain fatty acid production by .

Biotechnol Biofuels 2018 7;11:158. Epub 2018 Jun 7.

1UMR1319, Team BIMLip: Biologie Intégrative du Métabolisme Lipidique, Institut Micalis, INRA-AgroParisTech, Université Paris-Saclay, Domaine de Vilvert, 78352 Jouy-en-Josas, France.

Background: Odd chain fatty acids (odd FAs) have a wide range of applications in therapeutic and nutritional industries, as well as in chemical industries including biofuel. is an oleaginous yeast considered a preferred microorganism for the production of lipid-derived biofuels and chemicals. However, it naturally produces negligible amounts of odd chain fatty acids. Read More

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Biomimetic strategy for constructing cellulosomal operons in .

Biotechnol Biofuels 2018 7;11:157. Epub 2018 Jun 7.

5Department of Life Sciences, National Chung Hsing University, Taichung, 40227 Taiwan.

Background: Enzymatic conversion of lignocellulosic biomass into soluble sugars is a major bottleneck in the plant biomass utilization. Several anaerobic organisms cope these issues via multiple-enzyme complex system so called 'cellulosome'. Hence, we proposed a "biomimic operon" concept for making an artificial cellulosome which can be used as a promising tool for the expression of cellulosomal enzymes in . Read More

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The synchronous TAG production with the growth by the expression of chloroplast transit peptide-fused ScPDAT in .

Biotechnol Biofuels 2018 6;11:156. Epub 2018 Jun 6.

2Marine Bioengineering Group, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023 China.

Background: The synchronous triacylglycerol (TAG) production with the growth is a key step to lower the cost of the microalgae-based biofuel production. Phospholipid: diacylglycerol acyltransferase (PDAT) has been identified recently and catalyzes the phospholipid contributing acyl group to diacylglycerol to synthesize TAG, and is considered as the important source of TAG in .

Results: Using a chimeric Hsp70A-RbcS2 promoter, exogenous PDAT form fused with a chloroplast transit peptide was expressed in CC-137. Read More

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June 2018
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Genetic tool development and systemic regulation in biosynthetic technology.

Biotechnol Biofuels 2018 1;11:152. Epub 2018 Jun 1.

1State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Puzhu South Road 30#, Nanjing, 211800 People's Republic of China.

With the increased development in research, innovation, and policy interest in recent years, biosynthetic technology has developed rapidly, which combines engineering, electronics, computer science, mathematics, and other disciplines based on classical genetic engineering and metabolic engineering. It gives a wider perspective and a deeper level to perceive the nature of life via cell mechanism, regulatory networks, or biological evolution. Currently, synthetic biology has made great breakthrough in energy, chemical industry, and medicine industries, particularly in the programmable genetic control at multiple levels of regulation to perform designed goals. Read More

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Recent advances in bio-based multi-products of agricultural Jerusalem artichoke resources.

Biotechnol Biofuels 2018 1;11:151. Epub 2018 Jun 1.

1College of Food Science and Light Industry, Nanjing Tech University, Nanjing, 211816 China.

The Jerusalem artichoke is a perennial plant that belongs to the sunflower family. As a non-grain crop, Jerusalem artichoke possesses a number of desirable characteristics that make it a valuable feedstock for biorefinery, such as inulin content, rapid growth, strong adaptability, and high yields. This review provides a comprehensive introduction to renewable Jerusalem artichoke-based biomass resources and recent advances in bio-based product conversion. Read More

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An engineered fatty acid synthase combined with a carboxylic acid reductase enables de novo production of 1-octanol in .

Biotechnol Biofuels 2018 1;11:150. Epub 2018 Jun 1.

1Faculty of Biological Sciences, Institute of Molecular Bioscience, Goethe University Frankfurt, Max-von-Laue-Str. 9, 60438 Frankfurt, Germany.

Background: The ideal biofuel should not only be a regenerative fuel from renewable feedstocks, but should also be compatible with the existing fuel distribution infrastructure and with normal car engines. As the so-called drop-in biofuel, the fatty alcohol 1-octanol has been described as a valuable substitute for diesel and jet fuels and has already been produced fermentatively from sugars in small amounts with engineered bacteria via reduction of thioesterase-mediated premature release of octanoic acid from fatty acid synthase or via a reversal of the β-oxidation pathway.

Results: The previously engineered short-chain acyl-CoA producing yeast Fas1/Fas2 fatty acid synthase variant was expressed together with carboxylic acid reductase from and phosphopantetheinyl transferase Sfp from in a mutant strain. Read More

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Single-cell computational analysis of light harvesting in a flat-panel photo-bioreactor.

Biotechnol Biofuels 2018 26;11:149. Epub 2018 May 26.

1Forschungszentrum Jülich GmbH, Institute of Bio- and Geosciences, IBG-1: Biotechnology, Wilhelm-Johnen-Straße, 52428 Jülich, Germany.

Background: Flat-panel photo-bioreactors (PBRs) are customarily applied for investigating growth of microalgae. Optimal design and operation of such reactors is still a challenge due to complex non-linear combinations of various impact factors, particularly hydrodynamics, light irradiation, and cell metabolism. A detailed analysis of single-cell light reception can lead to novel insights into the complex interactions of light exposure and algae movement in the reactor. Read More

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Systems analysis of phosphate-limitation-induced lipid accumulation by the oleaginous yeast .

Biotechnol Biofuels 2018 25;11:148. Epub 2018 May 25.

1Division of Biotechnology, Dalian Institute of Chemical Physics, CAS, 457 Zhongshan Road, Dalian, 116023 People's Republic of China.

Background: Lipid accumulation by oleaginous microorganisms is of great scientific interest and biotechnological potential. While nitrogen limitation has been routinely employed, low-cost raw materials usually contain rich nitrogenous components, thus preventing from efficient lipid production. Inorganic phosphate (Pi) limitation has been found sufficient to promote conversion of sugars into lipids, yet the molecular basis of cellular response to Pi limitation and concurrent lipid accumulation remains elusive. Read More

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Yeast lipids from cardoon stalks, stranded driftwood and olive tree pruning residues as possible extra sources of oils for producing biofuels and biochemicals.

Biotechnol Biofuels 2018 23;11:147. Epub 2018 May 23.

1Department of Agricultural, Food and Environmental Sciences, Industrial Yeasts Collection DBVPG, University of Perugia, Perugia, Italy.

Background: Some lignocellulosic biomass feedstocks occur in Mediterranean Countries. They are still largely unexploited and cause considerable problems due to the lack of cost-effective harvesting, storage and disposal technologies. Recent studies found that some basidiomycetous yeasts are able to accumulate high amount of intracellular lipids for biorefinery processes (i. Read More

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Use of bacteria for improving the lignocellulose biorefinery process: importance of pre-erosion.

Biotechnol Biofuels 2018 23;11:146. Epub 2018 May 23.

1School of Metallurgy and Environment, Central South University, Changsha, 410083 China.

Background: Biological pretreatment is an important alternative strategy for biorefining lignocellulose and has attracted increasing attention in recent years. However, current designs for this pretreatment mainly focus on using various white rot fungi, overlooking the bacteria. To the best of our knowledge, for the first time, we evaluated the potential contribution of bacteria to lignocellulose pretreatment, with and without a physicochemical process, based on the bacterial strain sp. Read More

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Effect of oxygen mass transfer rate on the production of 2,3-butanediol from glucose and agro-industrial byproducts by ATCC9789.

Biotechnol Biofuels 2018 23;11:145. Epub 2018 May 23.

Department of Civil, Chemical, Environmental and Materials Engineering, University of Bologna, Via Terracini 28, 40131 Bologna, Italy.

Background: 2,3-Butanediol (BD) is a largely used fossil-based platform chemical. The yield and productivity of bio-based BD fermentative production must be increased and cheaper substrates need to be identified, to make bio-based BD production more competitive. As BD bioproduction occurs under microaerobic conditions, a fine tuning and control of the oxygen transfer rate (OTR) is crucial to maximize BD yield and productivity. Read More

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Determination of optimal biomass pretreatment strategies for biofuel production: investigation of relationships between surface-exposed polysaccharides and their enzymatic conversion using carbohydrate-binding modules.

Biotechnol Biofuels 2018 18;11:144. Epub 2018 May 18.

1Centre de recherche sur les matériaux lignocellulosiques, Université du Québec à Trois-Rivières, C.P. 500, Trois-Rivières, QC G9A 5H7 Canada.

Background: Pretreatment of lignocellulosic biomass (LCB) is a key step for its efficient bioconversion into ethanol. Determining the best pretreatment and its parameters requires monitoring its impacts on the biomass material. Here, we used fluorescent protein-tagged carbohydrate-binding modules method (FTCM)-depletion assay to study the relationship between surface-exposed polysaccharides and enzymatic hydrolysis of LCB. Read More

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Integrated experimental and technoeconomic evaluation of two-stage Cu-catalyzed alkaline-oxidative pretreatment of hybrid poplar.

Biotechnol Biofuels 2018 17;11:143. Epub 2018 May 17.

1DOE Great Lakes Bioenergy Research Center, Michigan State University, 1129 Farm Lane, East Lansing, MI 48824 USA.

Background: When applied to recalcitrant lignocellulosic feedstocks, multi-stage pretreatments can provide more processing flexibility to optimize or balance process outcomes such as increasing delignification, preserving hemicellulose, and maximizing enzymatic hydrolysis yields. We previously reported that adding an alkaline pre-extraction step to a copper-catalyzed alkaline hydrogen peroxide (Cu-AHP) pretreatment process resulted in improved sugar yields, but the process still utilized relatively high chemical inputs (catalyst and HO) and enzyme loadings. We hypothesized that by increasing the temperature of the alkaline pre-extraction step in water or ethanol, we could reduce the inputs required during Cu-AHP pretreatment and enzymatic hydrolysis without significant loss in sugar yield. Read More

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Identification of two integration sites in favor of transgene expression in .

Biotechnol Biofuels 2018 17;11:142. Epub 2018 May 17.

2State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101 China.

Background: The ascomycete fungus was widely used as a biotechnological workhorse for production of cellulases and recombinant proteins due to its large capacity of protein secretion. Transgenesis by random integration of a gene of interest (GOI) into the genome of can generate series of strains that express different levels of the indicated transgene. The insertion site of the GOI plays an important role in the ultimate production of the targeted proteins. Read More

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Developing cellulolytic as a platform for the production of valuable products in consolidated bioprocessing of cellulose.

Biotechnol Biofuels 2018 15;11:141. Epub 2018 May 15.

LISBP, CNRS, INSA/INRA UMR 792, Université de Toulouse, 135, Avenue de Rangueil, 31077 Toulouse, France.

Background: Both industrial biotechnology and the use of cellulosic biomass as feedstock for the manufacture of various commercial goods are prominent features of the bioeconomy. In previous work, with the aim of developing a consolidated bioprocess for cellulose bioconversion, we conferred cellulolytic activity of , one of the most widely studied "nonconventional" oleaginous yeast species. However, further engineering this strain often leads to the loss of previously introduced heterologous genes due to the presence of multiple sites when using -recombinase to remove previously employed selection markers. Read More

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Promiscuous activities of heterologous enzymes lead to unintended metabolic rerouting in engineered to assimilate various sugars from renewable biomass.

Biotechnol Biofuels 2018 14;11:140. Epub 2018 May 14.

1Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, IL 61801 USA.

Background: Understanding the global metabolic network, significantly perturbed upon promiscuous activities of foreign enzymes and different carbon sources, is crucial for systematic optimization of metabolic engineering of yeast . Here, we studied the effects of promiscuous activities of overexpressed enzymes encoded by foreign genes on rerouting of metabolic fluxes of an engineered yeast capable of assimilating sugars from renewable biomass by profiling intracellular and extracellular metabolites.

Results: Unbiased metabolite profiling of the engineered strain EJ4 revealed promiscuous enzymatic activities of xylose reductase and xylitol dehydrogenase on galactose and galactitol, respectively, resulting in accumulation of galactitol and tagatose during galactose fermentation. Read More

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Life cycle assessment of residual lignocellulosic biomass-based jet fuel with activated carbon and lignosulfonate as co-products.

Biotechnol Biofuels 2018 14;11:139. Epub 2018 May 14.

Center for International Trade in Forest Products, School of Environmental and Forest Sciences, University of Washington, Seattle, WA 98195 USA.

Background: Bio-jet fuels are emerging as a valuable alternative to petroleum-based fuels for their potential for reducing greenhouse gas emissions and fossil fuel dependence. In this study, residual woody biomass from slash piles in the U.S. Read More

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Continuous succinic acid fermentation by in a packed-bed biofilm reactor.

Biotechnol Biofuels 2018 14;11:138. Epub 2018 May 14.

1Dipartimento di Ingegneria Chimica, dei Materiali e della Produzione Industriale, Università degli Studi di Napoli Federico II, P.le V. Tecchio 80, 80125 Naples, Italy.

Background: Succinic acid is one of the most interesting platform chemicals that can be produced in a biorefinery approach. In this study, continuous succinic acid production by fermentation in a packed-bed biofilm reactor (PBBR) was investigated.

Results: The effects of the operating conditions tested, dilution rate (D), and medium composition (mixture of glucose, xylose, and arabinose-that simulate the composition of a lignocellulosic hydrolysate)-on the PBBR performances were investigated. Read More

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One-step process of hydrothermal and alkaline treatment of wheat straw for improving the enzymatic saccharification.

Biotechnol Biofuels 2018 12;11:137. Epub 2018 May 12.

2Beijing Key Laboratory of Lignocellulosic Chemistry, Beijing Forestry University, Beijing, 100083 China.

Background: To increase the production of bioethanol, a two-step process based on hydrothermal and dilute alkaline treatment was applied to reduce the natural resistance of biomass. However, the process required a large amount of water and a long operation time due to the solid/liquid separation before the alkaline treatment, which led to decrease the pure economic profit for production of bioethanol. Therefore, four one-step processes based on order of hydrothermal and alkaline treatment have been developed to enhance concentration of glucose of wheat straw by enzymatic saccharification. Read More

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Bioreactor microbial ecosystems with differentiated methanogenic phenol biodegradation and competitive metabolic pathways unraveled with genome-resolved metagenomics.

Biotechnol Biofuels 2018 11;11:135. Epub 2018 May 11.

1Environmental Biotechnology Lab, The University of Hong Kong SAR, Pokfulam Road, Hong Kong, China.

Background: Methanogenic biodegradation of aromatic compounds depends on syntrophic metabolism. However, metabolic enzymes and pathways of uncultured microorganisms and their ecological interactions with methanogenic consortia are unknown because of their resistance to isolation and limited genomic information.

Results: Genome-resolved metagenomics approaches were used to reconstruct and dissect 23 prokaryotic genomes from 37 and 20 °C methanogenic phenol-degrading reactors. Read More

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May 2018
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A efflux pump acts on short-chain alcohols.

Biotechnol Biofuels 2018 11;11:136. Epub 2018 May 11.

1Department of Chemical and Biomolecular Engineering, University of California, Berkeley, CA USA.

Background: The microbial production of biofuels is complicated by a tradeoff between yield and toxicity of many fuels. Efflux pumps enable bacteria to tolerate toxic substances by their removal from the cells while bypassing the periplasm. Their use for the microbial production of biofuels can help to improve cell survival, product recovery, and productivity. Read More

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Integrated in situ gas stripping-salting-out process for high-titer acetone-butanol-ethanol production from sweet sorghum bagasse.

Biotechnol Biofuels 2018 10;11:134. Epub 2018 May 10.

1National Energy R&D Center for Biorefinery, Beijing University of Chemical Technology, No. 15 Beisanhuan East Road, Chaoyang District, Beijing, 100029 People's Republic of China.

Background: The production of biobutanol from renewable biomass resources is attractive. The energy-intensive separation process and low-titer solvents production are the key constraints on the economy-feasible acetone-butanol-ethanol (ABE) production by fermentation. To decrease energy consumption and increase the solvents concentration, a novel two-stage gas stripping-salting-out system was established for effective ABE separation from the fermentation broth using sweet sorghum bagasse as feedstock. Read More

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A downstream box fusion allows stable accumulation of a bacterial cellulase in chloroplasts.

Biotechnol Biofuels 2018 10;11:133. Epub 2018 May 10.

1Department of Biological and Environmental Engineering, Cornell University, 111 Wing Drive, Ithaca, NY USA.

Background: We investigated strategies to improve foreign protein accumulation in the chloroplasts of the model algae and tested the outcome in both standard culture conditions as well as one pertinent to algal biofuel production. The downstream box (DB) of the or genes, the first 15 codons following the start codon, was -terminally fused to the coding region of , an endoglucanase from . We also employed a chimeric regulatory element, consisting of the rRNA promoter and the 5'UTR, previously reported to enhance protein expression, to regulate the expression of the TetC- gene. Read More

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Rich biotin content in lignocellulose biomass plays the key role in determining cellulosic glutamic acid accumulation by .

Biotechnol Biofuels 2018 10;11:132. Epub 2018 May 10.

State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237 China.

Background: Lignocellulose is one of the most promising alternative feedstocks for glutamic acid production as commodity building block chemical, but the efforts by the dominant industrial fermentation strain failed for accumulating glutamic acid using lignocellulose feedstock.

Results: We identified the existence of surprisingly high biotin concentration in corn stover hydrolysate as the determining factor for the failure of glutamic acid accumulation by . Under excessive biotin content, induction by penicillin resulted in 41. Read More

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High-oleate yeast oil without polyunsaturated fatty acids.

Biotechnol Biofuels 2018 9;11:131. Epub 2018 May 9.

Novogy, Inc, 85 Bolton Street, Cambridge, MA 02140 USA.

Background: Oleate-enriched triacylglycerides are well-suited for lubricant applications that require high oxidative stability. Fatty acid carbon chain length and degree of desaturation are key determinants of triacylglyceride properties and the ability to manipulate fatty acid composition in living organisms is critical to developing a source of bio-based oil tailored to meet specific application requirements.

Results: We sought to engineer the oleaginous yeast for production of high-oleate triacylglyceride oil. Read More

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May 2018
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Engineering and systems-level analysis of for production of phenazine-1-carboxamide using glycerol as the cost-effective carbon source.

Biotechnol Biofuels 2018 4;11:130. Epub 2018 May 4.

1State Key Laboratory of Microbial Metabolism, and School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai, 200240 China.

Background: Glycerol, an inevitable byproduct of biodiesel, has become an attractive feedstock for the production of value-added chemicals due to its availability and low price. HT66 can use glycerol to synthesize phenazine-1-carboxamide (PCN), a phenazine derivative, which is strongly antagonistic to fungal phytopathogens. A systematic understanding of underlying mechanisms for the PCN overproduction will be important for the further improvement and industrialization. Read More

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May 2018
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Enhanced biological fixation of methane for microbial lipid production by recombinant .

Biotechnol Biofuels 2018 4;11:129. Epub 2018 May 4.

2National Bioenergy Center, National Renewable Energy Laboratory, Golden, CO USA.

Background: Due to the success of shale gas development in the US, the production cost of natural gas has been reduced significantly, which in turn has made methane (CH), the major component of natural gas, a potential alternative substrate for bioconversion processes compared with other high-price raw material sources or edible feedstocks. Therefore, exploring effective ways to use CH for the production of biofuels is attractive. Biological fixation of CH by methanotrophic bacteria capable of using CH as their sole carbon and energy source has obtained great attention for biofuel production from this resource. Read More

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A novel close-circulating vapor stripping-vapor permeation technique for boosting biobutanol production and recovery.

Biotechnol Biofuels 2018 4;11:128. Epub 2018 May 4.

School of Life Science and Biotechnology, Dalian University of Technology, No 2 Linggong Road, Dalian, 116024 China.

Background: Butanol derived from renewable resources by microbial fermentation is considered as one of not only valuable platform chemicals but alternative advanced biofuels. However, due to low butanol concentration in fermentation broth, butanol production is restricted by high energy consumption for product recovery. For in situ butanol recovery techniques, such as gas stripping and pervaporation, the common problem is their low efficiency in harvesting and concentrating butanol. Read More

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Ethanol production potential from AFEX™ and steam-exploded sugarcane residues for sugarcane biorefineries.

Biotechnol Biofuels 2018 4;11:127. Epub 2018 May 4.

1Department of Process Engineering, Stellenbosch University, Private Bag X1 Matieland, Stellenbosch, South Africa.

Background: Expanding biofuel markets are challenged by the need to meet future biofuel demands and mitigate greenhouse gas emissions, while using domestically available feedstock sustainably. In the context of the sugar industry, exploiting under-utilized cane leaf matter (CLM) in addition to surplus sugarcane bagasse as supplementary feedstock for second-generation ethanol production has the potential to improve bioenergy yields per unit land. In this study, the ethanol yields and processing bottlenecks of ammonia fibre expansion (AFEX™) and steam explosion (StEx) as adopted technologies for pretreating sugarcane bagasse and CLM were experimentally measured and compared for the first time. Read More

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An endogenous microRNA (miRNA1166.1) can regulate photobio-H production in eukaryotic green alga .

Biotechnol Biofuels 2018 2;11:126. Epub 2018 May 2.

1Guangdong Technology Research Center for Marine Algal Bioengineering, Guangdong Key Laboratory of Plant Epigenetic, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen, 518060 People's Republic of China.

Background: Hydrogen photoproduction from green microalgae is regarded as a promising alternative solution for energy problems. However, the simultaneous oxygen evolution from microalgae can prevent continuous hydrogen production due to the hypersensitivity of hydrogenases to oxygen. Sulfur deprivation can extend the duration of algal hydrogen production, but it is uneconomical to alternately culture algal cells in sulfur-sufficient and sulfur-deprived media. Read More

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Complete genome sequence and the expression pattern of plasmids of the model ethanologen ZM4 and its xylose-utilizing derivatives 8b and 2032.

Biotechnol Biofuels 2018 2;11:125. Epub 2018 May 2.

3DOE-Great Lakes Bioenergy Research Center (GLBRC), University of Wisconsin-Madison, Madison, WI USA.

Background: is a natural ethanologen being developed and deployed as an industrial biofuel producer. To date, eight strains have been completely sequenced and found to contain 2-8 native plasmids. However, systematic verification of predicted plasmid genes and their contribution to cell fitness has not been hitherto addressed. Read More

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CIPK9 is involved in seed oil regulation in L. and (L.) Heynh.

Biotechnol Biofuels 2018 2;11:124. Epub 2018 May 2.

1National Key Laboratory of Crop Genetic Improvement/National Engineering Research Center of Rapeseed, Huazhong Agricultural University, Wuhan, 430070 China.

Background: Accumulation of storage compounds during seed development plays an important role in the life cycle of oilseed plants; these compounds provide carbon and energy resources to support the establishment of seedlings.

Results: In this study, we show that has a broad expression pattern in L. tissues and that wounding stress strongly induces its expression. Read More

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Methane-yielding microbial communities processing lactate-rich substrates: a piece of the anaerobic digestion puzzle.

Biotechnol Biofuels 2018 21;11:116. Epub 2018 Apr 21.

1Department of Molecular Biology, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland.

Background: Anaerobic digestion, whose final products are methane and carbon dioxide, ensures energy flow and circulation of matter in ecosystems. This naturally occurring process is used for the production of renewable energy from biomass. Lactate, a common product of acidic fermentation, is a key intermediate in anaerobic digestion of biomass in the environment and biogas plants. Read More

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The β-glucosidase secreted by under carbon starvation: a versatile catalyst for biofuel production from plant and algal biomass.

Biotechnol Biofuels 2018 27;11:123. Epub 2018 Apr 27.

Department of Microbial and Plant Biotechnology, Centro de Investigaciones Biológicas, CSIC, Ramiro de Maeztu 9, 28040 Madrid, Spain.

Background: In the last years, the most outstanding trend for obtaining high added-value components and second-generation (2G) biofuels consisted on exploitation of plant biomass. But recently, 3G biofuels, based in algae biomass, have emerged as a great alternative for production of energy.

Results: In this work, a versatile β-glucosidase from the ascomycete fungus has been purified, characterized, and heterologously expressed. Read More

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Switchgrass ( L.) promoters for green tissue-specific expression of the transcription factor for reduced-recalcitrance transgenic switchgrass.

Biotechnol Biofuels 2018 24;11:122. Epub 2018 Apr 24.

1Department of Plant Sciences, University of Tennessee, Knoxville, TN USA.

Background: Genetic engineering of switchgrass ( L.) for reduced cell wall recalcitrance and improved biofuel production has been a long pursued goal. Up to now, constitutive promoters have been used to direct the expression of cell wall biosynthesis genes toward attaining that goal. Read More

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April 2018
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Meta-proteomic analysis of protein expression distinctive to electricity-generating biofilm communities in air-cathode microbial fuel cells.

Biotechnol Biofuels 2018 23;11:121. Epub 2018 Apr 23.

1Department of Chemical and Biological Engineering, Colorado State University, Fort Collins, USA.

Background: Bioelectrochemical systems (BESs) harness electrons from microbial respiration to generate power or chemical products from a variety of organic feedstocks, including lignocellulosic biomass, fermentation byproducts, and wastewater sludge. In some BESs, such as microbial fuel cells (MFCs), bacteria living in a biofilm use the anode as an electron acceptor for electrons harvested from organic materials such as lignocellulosic biomass or waste byproducts, generating energy that may be used by humans. Many BES applications use bacterial biofilm communities, but no studies have investigated protein expression by the anode biofilm community as a whole. Read More

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April 2018
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AC-202, a highly effective fluorophore for the visualization of lipid droplets in green algae and diatoms.

Biotechnol Biofuels 2018 23;11:120. Epub 2018 Apr 23.

Aix Marseille Univ, CEA, CNRS, UMR7265 BVME, 13009 Marseille, France.

Background: Lipid-specific live cell dyes are an important tool for the study of algal lipid metabolism, the monitoring of lipid production, and the identification of algal strains with high lipid yields. Nile Red and BODIPY have emerged as the principal dyes for these purposes. However, they suffer from a number of shortcomings including for specificity, penetration, interference from chlorophyll autofluorescence, and incompatibility with widely used genetically encoded reporters in the green and blue regions of the spectrum such as the green fluorescent protein and the red fluorescent protein. Read More

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Enhanced extraction of butyric acid under high-pressure CO conditions to integrate chemical catalysis for value-added chemicals and biofuels.

Biotechnol Biofuels 2018 23;11:119. Epub 2018 Apr 23.

Department of Chemical Engineering, Hanyang University, 222 Wangshimni-ro, Seongdong-gu, Seoul, 04763 South Korea.

Background: Extractive fermentation with the removal of carboxylic acid requires low pH conditions because acids are better partitioned into the solvent phase at low pH values. However, this requirement conflicts with the optimal near-neutral pH conditions for microbial growth.

Results: CO pressurization was used, instead of the addition of chemicals, to decrease pH for the extraction of butyric acid, a fermentation product of , and butyl butyrate was selected as an extractant. Read More

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Process analysis and optimization of simultaneous saccharification and co-fermentation of ethylenediamine-pretreated corn stover for ethanol production.

Biotechnol Biofuels 2018 23;11:118. Epub 2018 Apr 23.

1Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University, Weijin Road 92, Nankai District, Tianjin, 300072 People's Republic of China.

Background: Improving ethanol concentration and reducing enzyme dosage are main challenges in bioethanol refinery from lignocellulosic biomass. Ethylenediamine (EDA) pretreatment is a novel method to improve enzymatic digestibility of lignocellulose. In this study, simultaneous saccharification and co-fermentation (SSCF) process using EDA-pretreated corn stover was analyzed and optimized to verify the constraint factors on ethanol production. Read More

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Genome-centric metatranscriptomes and ecological roles of the active microbial populations during cellulosic biomass anaerobic digestion.

Biotechnol Biofuels 2018 23;11:117. Epub 2018 Apr 23.

B5423-AC1, School of Energy and Environment, City University of Hong Kong, Tat Chee Avenue, Kowloon, Hong Kong.

Background: Although anaerobic digestion for biogas production is used worldwide in treatment processes to recover energy from carbon-rich waste such as cellulosic biomass, the activities and interactions among the microbial populations that perform anaerobic digestion deserve further investigations, especially at the population genome level. To understand the cellulosic biomass-degrading potentials in two full-scale digesters, this study examined five methanogenic enrichment cultures derived from the digesters that anaerobically digested cellulose or xylan for more than 2 years under 35 or 55 °C conditions.

Results: Metagenomics and metatranscriptomics were used to capture the active microbial populations in each enrichment culture and reconstruct their meta-metabolic network and ecological roles. Read More

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Engineering global transcription to tune lipophilic properties in .

Biotechnol Biofuels 2018 19;11:115. Epub 2018 Apr 19.

1Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072 People's Republic of China.

Background: Evolution of complex phenotypes in cells requires simultaneously tuning expression of large amounts of genes, which can be achieved by reprograming global transcription. Lipophilicity is an important complex trait in oleaginous yeast . It is necessary to explore the changes of which genes' expression levels will tune cellular lipophilic properties via the strategy of global transcription engineering. Read More

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Mannanase hydrolysis of spruce galactoglucomannan focusing on the influence of acetylation on enzymatic mannan degradation.

Biotechnol Biofuels 2018 19;11:114. Epub 2018 Apr 19.

1Division of Industrial Biotechnology, Department of Biology and Biological Engineering, Chalmers University of Technology, 412 96 Gothenburg, Sweden.

Background: Galactoglucomannan (GGM) is the most abundant hemicellulose in softwood, and consists of a backbone of mannose and glucose units, decorated with galactose and acetyl moieties. GGM can be hydrolyzed into fermentable sugars, or used as a polymer in films, gels, and food additives. -β-mannanases, which can be found in the glycoside hydrolase families 5 and 26, specifically cleave the mannan backbone of GGM into shorter oligosaccharides. Read More

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Characterisation of an efficient atrazine-degrading bacterium, sp. ZXY-2: an attempt to lay the foundation for potential bioaugmentation applications.

Biotechnol Biofuels 2018 18;11:113. Epub 2018 Apr 18.

State Key Laboratory of Urban Water Resource and Environment and Environment, School of Environment, Harbin Institute of Technology, Harbin, 150090 China.

Background: The isolation of atrazine-degrading microorganisms with specific characteristics is fundamental for bioaugmenting the treatment of wastewater containing atrazine. However, studies describing the specific features of such microorganisms are limited, and further investigation is needed to improve our understanding of bioaugmentation.

Results And Conclusions: In this study, strain sp. Read More

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Disruption of the transcription factors Thi2p and Nrm1p alleviates the post-glucose effect on xylose utilization in .

Biotechnol Biofuels 2018 16;11:112. Epub 2018 Apr 16.

1State Key Laboratory of Microbial Technology, Microbiology and Biotechnology Institute, Shandong University, Shan Da Nan Road 27, Jinan, 250100 China.

Background: The recombinant strains that acquired the ability to utilize xylose through metabolic and evolutionary engineering exhibit good performance when xylose is the sole carbon source in the medium (designated the X stage in the present work). However, the xylose consumption rate of strains is generally low after glucose depletion during glucose-xylose co-fermentation, despite the presence of xylose in the medium (designated the GX stage in the present work). Glucose fermentation appears to reduce the capacity of these strains to "recognize" xylose during the GX stage, a phenomenon termed the post-glucose effect on xylose metabolism. Read More

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Determinants on an efficient cellulase recycling process for the production of bioethanol from recycled paper sludge under high solid loadings.

Biotechnol Biofuels 2018 16;11:111. Epub 2018 Apr 16.

Centre of Biological Engineering, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal.

Background: In spite of the continuous efforts and investments in the last decades, lignocellulosic ethanol is still not economically competitive with fossil fuels. Optimization is still required in different parts of the process. Namely, the cost effective usage of enzymes has been pursued by different strategies, one of them being recycling. Read More

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Glycoside hydrolase gene transcription by during growth on wheat arabinoxylan and monosaccharides: a proposed xylan hydrolysis mechanism.

Biotechnol Biofuels 2018 16;11:110. Epub 2018 Apr 16.

3Department of Biology, New Mexico Institute of Mining and Technology, 801 Leroy Pl, Socorro, NM 87801 USA.

Background: Metabolism of carbon bound in wheat arabinoxylan (WAX) polysaccharides by bacteria requires a number of glycoside hydrolases active toward different bonds between sugars and other molecules. is a Gram-positive thermoacidophilic bacterium capable of growth on a variety of mono-, di-, oligo-, and polysaccharides. Nineteen proposed glycoside hydrolases have been annotated in the Type Strain ATCC27009/DSM 446 genome. Read More

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Visualizing cellulase adsorption and quantitatively determining cellulose accessibility with an updated fungal cellulose-binding module-based fluorescent probe protein.

Biotechnol Biofuels 2018 9;11:105. Epub 2018 Apr 9.

Key Laboratory for Industrial Biocatalysis, Ministry of Education of China, Institute of Applied Chemistry, Department of Chemical Engineering, Tsinghua University, Beijing, 100084 China.

Background: Cellulose accessibility to cellulases (CAC) is a direct factor determining the enzymatic digestibility of lignocellulosic cellulose. Improving CAC by pretreatment is a prerequisite step for the efficient release of fermentable sugars from biomass cell wall. However, conventional methods to study the porosimetry of solid materials showed some limitations to be used for investigating CAC. Read More

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April 2018
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