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    1331 results match your criteria Biotechnology for Biofuels [Journal]

    1 OF 27

    A novel, highly efficient β-glucosidase with a cellulose-binding domain: characterization and properties of native and recombinant proteins.
    Biotechnol Biofuels 2017 6;10:256. Epub 2017 Nov 6.
    Department of Environmental Biology, Centro de Investigaciones Biológicas, CSIC, Ramiro de Maeztu 9, 28040 Madrid, Spain.
    Background: Cellulose, the most abundant biopolymer on earth, is an alternative for fossil fuels as a renewable feedstock for the production of second-generation biofuels and other chemicals. The discovery of novel, highly efficient β-glucosidases remains as one of the major bottlenecks for cellulose degradation. In this context, the ascomycete Talaromyces amestolkiae, isolated from cereal samples, has been studied as a promising source for these enzymes. Read More

    Targeted metatranscriptomics of compost-derived consortia reveals a GH11 exerting an unusual exo-1,4-β-xylanase activity.
    Biotechnol Biofuels 2017 2;10:254. Epub 2017 Nov 2.
    Instituto de Física de São Carlos, Universidade de São Paulo, Av. Trabalhador São-carlense 400, São Carlos, SP 13560-970 Brazil.
    Background: Using globally abundant crop residues as a carbon source for energy generation and renewable chemicals production stand out as a promising solution to reduce current dependency on fossil fuels. In nature, such as in compost habitats, microbial communities efficiently degrade the available plant biomass using a diverse set of synergistic enzymes. However, deconstruction of lignocellulose remains a challenge for industry due to recalcitrant nature of the substrate and the inefficiency of the enzyme systems available, making the economic production of lignocellulosic biofuels difficult. Read More

    A seven-gene cluster in Ruminiclostridium cellulolyticum is essential for signalization, uptake and catabolism of the degradation products of cellulose hydrolysis.
    Biotechnol Biofuels 2017 30;10:250. Epub 2017 Oct 30.
    Aix Marseille Univ, CNRS, LCB, Marseille, France.
    Background: Like a number of anaerobic and cellulolytic Gram-positive bacteria, the model microorganism Ruminiclostridium cellulolyticum produces extracellular multi-enzymatic complexes called cellulosomes, which efficiently degrade the crystalline cellulose. Action of the complexes on cellulose releases cellobiose and longer cellodextrins but to date, little is known about the transport and utilization of the produced cellodextrins in the bacterium. A better understanding of the uptake systems and fermentation of sugars derived from cellulose could have a major impact in the field of biofuels production. Read More

    Hybrid photosynthesis-powering biocatalysts with solar energy captured by inorganic devices.
    Biotechnol Biofuels 2017 30;10:249. Epub 2017 Oct 30.
    School of Chemistry, Chemical Engineering and Life Science, Wuhan University of Technology, Wuhan, 430070 People's Republic of China.
    The biological reduction of CO2 driven by sunlight via photosynthesis is a crucial process for life on earth. However, the conversion efficiency of solar energy to biomass by natural photosynthesis is low. This translates in bioproduction processes relying on natural photosynthesis that are inefficient energetically. Read More

    Impairment of NADH dehydrogenase and regulation of anaerobic metabolism by the small RNA RyhB and NadE for improved biohydrogen production in Enterobacter aerogenes.
    Biotechnol Biofuels 2017 30;10:248. Epub 2017 Oct 30.
    Key Lab of Industrial Biocatalysis, Ministry of Education, Department of Chemical Engineering, Tsinghua University, Beijing, 100084 China.
    Background: Enterobacter aerogenes is a facultative anaerobe and is one of the most widely studied bacterial strains because of its ability to use a variety of substrates, to produce hydrogen at a high rate, and its high growth rate during dark fermentation. However, the rate of hydrogen production has not been optimized. In this present study, three strategies to improve hydrogen production in E. Read More

    Oleaginous yeast Yarrowia lipolytica culture with synthetic and food waste-derived volatile fatty acids for lipid production.
    Biotechnol Biofuels 2017 30;10:247. Epub 2017 Oct 30.
    School of Energy and Environmental Engineering, Beijing Key Laboratory of Resource-oriented Treatment of Industrial Pollutants, University of Science and Technology Beijing, Beijing, 100083 People's Republic of China.
    Background: The sustainability of microbial lipids production from traditional carbon sources, such as glucose or glycerol, is problematic given the high price of raw materials. Considerable efforts have been directed to minimize the cost and find new alternative carbon sources. Volatile fatty acids (VFAs) are especially attractive raw materials, because they can be produced from a variety of organic wastes fermentation. Read More

    Bioprospecting of novel thermostable β-glucosidase from Bacillus subtilis RA10 and its application in biomass hydrolysis.
    Biotechnol Biofuels 2017 30;10:246. Epub 2017 Oct 30.
    Enzyme Technology and Protein Bioinformatics Laboratory, Department of Microbiology, Maharshi Dayanand University, Rohtak, Haryana 124001 India.
    Background: Saccharification is the most crucial and cost-intensive process in second generation biofuel production. The deficiency of β-glucosidase in commercial enzyme leads to incomplete biomass hydrolysis. The decomposition of biomass at high temperature environments leads us to isolate thermotolerant microbes with β-glucosidase production potential. Read More

    Modular pathway engineering for the microbial production of branched-chain fatty alcohols.
    Biotechnol Biofuels 2017 27;10:244. Epub 2017 Oct 27.
    Department of Energy, Environmental and Chemical Engineering, Washington University in St. Louis, 1 Brookings Drive, Campus Box 1180, Saint Louis, MO 63130 USA.
    The intrinsic structural properties of branched long-chain fatty alcohols (BLFLs) in the range of C12 to C18 make them more suitable as diesel fuel replacements and for other industrial applications than their straight-chain counterparts. While microbial production of straight long-chain fatty alcohols has been achieved, biosynthesis of BLFLs has never been reported. In this work, we engineered four different biosynthetic pathways in Escherichia coli to produce BLFLs. Read More

    Sustainable carbon sources for microbial organic acid production with filamentous fungi.
    Biotechnol Biofuels 2017 23;10:242. Epub 2017 Oct 23.
    Technical Biology, Institute of Process Engineering in Life Sciences, Karlsruhe Institute of Technology (KIT), Engler-Bunte-Ring 3, Karlsruhe, 76131 Germany.
    Background: The organic acid producer Aspergillus oryzae and Rhizopus delemar are able to convert several alternative carbon sources to malic and fumaric acid. Thus, carbohydrate hydrolysates from lignocellulose separation are likely suitable as substrate for organic acid production with these fungi.

    Results: Before lignocellulose hydrolysate fractions were tested as substrates, experiments with several mono- and disaccharides, possibly present in pretreated biomass, were conducted for their suitability for malic acid production with A. Read More

    Rhodosporidium toruloides: a new platform organism for conversion of lignocellulose into terpene biofuels and bioproducts.
    Biotechnol Biofuels 2017 23;10:241. Epub 2017 Oct 23.
    Joint BioEnergy Institute, 5885 Hollis St, Emeryville, CA 94608 USA.
    Background: Economical conversion of lignocellulosic biomass into biofuels and bioproducts is central to the establishment of a robust bioeconomy. This requires a conversion host that is able to both efficiently assimilate the major lignocellulose-derived carbon sources and divert their metabolites toward specific bioproducts.

    Results: In this study, the carotenogenic yeast Rhodosporidium toruloides was examined for its ability to convert lignocellulose into two non-native sesquiterpenes with biofuel (bisabolene) and pharmaceutical (amorphadiene) applications. Read More

    Comparative characterization of all cellulosomal cellulases from Clostridium thermocellum reveals high diversity in endoglucanase product formation essential for complex activity.
    Biotechnol Biofuels 2017 23;10:240. Epub 2017 Oct 23.
    Department of Microbiology, Technische Universität München, Emil-Ramann-Str. 4, 85354 Freising, Germany.
    Background: Clostridium thermocellum is a paradigm for efficient cellulose degradation and a promising organism for the production of second generation biofuels. It owes its high degradation rate on cellulosic substrates to the presence of supra-molecular cellulase complexes, cellulosomes, which comprise over 70 different single enzymes assembled on protein-backbone molecules of the scaffold protein CipA.

    Results: Although all 24 single-cellulosomal cellulases were described previously, we present the first comparative catalogue of all these enzymes together with a comprehensive analysis under identical experimental conditions, including enzyme activity, binding characteristics, substrate specificity, and product analysis. Read More

    Metabolic responses to ethanol and butanol in Chlamydomonas reinhardtii.
    Biotechnol Biofuels 2017 17;10:239. Epub 2017 Oct 17.
    Shenzhen Key Laboratory of Marine Bioresource and Eco-environmental Science, Shenzhen University, Shenzhen, 518060 People's Republic of China.
    Background: Microalgae have been demonstrated to be among the most promising phototrophic species for producing renewable biofuels and chemicals. Ethanol and butanol are clean energy sources with good chemical and physical properties as alternatives to gasoline. However, biosynthesis of these two biofuels has not been achieved due to low tolerance of algal cells to ethanol or butanol. Read More

    Pulse electromagnetic fields enhance extracellular electron transfer in magnetic bioelectrochemical systems.
    Biotechnol Biofuels 2017 16;10:238. Epub 2017 Oct 16.
    School of Environment, State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, 73 Huanghe Road, Nangang District, P.O. Box 2614, Harbin, 150090 Heilongjiang China.
    Background: Microbial extracellular electron transfer (EET) is essential in driving the microbial interspecies interaction and redox reactions in bioelectrochemical systems (BESs). Magnetite (Fe3O4) and magnetic fields (MFs) were recently reported to promote microbial EET, but the mechanisms of MFs stimulation of EET and current generation in BESs are not known. This study investigates the behavior of current generation and EET in a state-of-the-art pulse electromagnetic field (PEMF)-assisted magnetic BES (PEMF-MBES), which was equipped with magnetic carbon particle (Fe3O4@N-mC)-coated electrodes. Read More

    Replacing process water and nitrogen sources with biogas slurry during cellulosic ethanol production.
    Biotechnol Biofuels 2017 16;10:236. Epub 2017 Oct 16.
    Biomass Energy Technology Research Centre, Key Laboratory of Development and Application of Rural Renewable Energy (Ministry of Agriculture), Biogas Institute of Ministry of Agriculture, Section 4-13, Renmin Nanlu, Chengdu, 610041 People's Republic of China.
    Background: Environmental issues, such as the fossil energy crisis, have resulted in increased public attention to use bioethanol as an alternative renewable energy. For ethanol production, water and nutrient consumption has become increasingly important factors being considered by the bioethanol industry as reducing the consumption of these resources would decrease the overall cost of ethanol production. Biogas slurry contains not only large amounts of wastewater, but also the nutrients required for microbial growth, e. Read More

    Engineering Bacillus licheniformis as a thermophilic platform for the production of l-lactic acid from lignocellulose-derived sugars.
    Biotechnol Biofuels 2017 11;10:235. Epub 2017 Oct 11.
    Clinical and Translational Research Center, Shanghai First Maternity and Infant Hospital, Tongji University School of Medicine, Shanghai, 200092 People's Republic of China.
    Background: Bacillus licheniformis MW3 as a GRAS and thermophilic strain is a promising microorganism for chemical and biofuel production. However, its capacity to co-utilize glucose and xylose, the major sugars found in lignocellulosic biomass, is severely impaired by glucose-mediated carbon catabolite repression (CCR). In this study, a "dual-channel" process was implemented to engineer strain MW3 for simultaneous utilization of glucose and xylose, using l-lactic acid as a target product. Read More

    A new method to evaluate temperature vs. pH activity profiles for biotechnological relevant enzymes.
    Biotechnol Biofuels 2017 11;10:234. Epub 2017 Oct 11.
    Department of Microbiology, TUM School of Life Sciences Weihenstephan, Technical University of Munich, Emil-Ramann-Str. 4, 85354 Freising-Weihenstephan, Germany.
    Background: Glycoside hydrolases are important for various industrial and scientific applications. Determination of their temperature as well as pH optima and range is crucial to evaluate whether an enzyme is suitable for application in a biotechnological process. These basic characteristics of enzymes are generally determined by two separate measurements. Read More

    Conversion of cellulose and hemicellulose of biomass simultaneously to acetoin by thermophilic simultaneous saccharification and fermentation.
    Biotechnol Biofuels 2017 10;10:232. Epub 2017 Oct 10.
    National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, 100190 China.
    Background: Acetoin (3-hydroxy-2-butanone), the precursor of biofuel 2,3-butanediol, is an important bio-based platform chemical with wide applications. Fermenting the low-cost and renewable plant biomass is undoubtedly a promising strategy for acetoin production. Isothermal simultaneous saccharification and fermentation (SSF) is regarded as an efficient method for bioconversion of lignocellulosic biomass, in which the temperature optima fitting for both lignocellulose-degrading enzymes and microbial strains. Read More

    Increased lipid production by heterologous expression of AtWRI1 transcription factor in Nannochloropsis salina.
    Biotechnol Biofuels 2017 10;10:231. Epub 2017 Oct 10.
    Department of Chemical and Biomolecular Engineering, KAIST, 291, Daehak-ro, Yuseong-gu, Daejeon, 34141 Republic of Korea.
    Background: Genetic engineering of microalgae is necessary to produce economically feasible strains for biofuel production. Current efforts are focused on the manipulation of individual metabolic genes, but the outcomes are not sufficiently stable and/or efficient for large-scale production of biofuels and other materials. Transcription factors (TFs) are emerging as good alternatives for engineering of microalgae, not only to increase production of biomaterials but to enhance stress tolerance. Read More

    Genetic engineering of the Calvin cycle toward enhanced photosynthetic CO2 fixation in microalgae.
    Biotechnol Biofuels 2017 5;10:229. Epub 2017 Oct 5.
    Institute for Food and Bioresource Engineering, College of Engineering, Peking University, Beijing, 100871 China.
    Background: Photosynthetic microalgae are emerging as potential biomass feedstock for sustainable production of biofuels and value-added bioproducts. CO2 biomitigation through these organisms is considered as an eco-friendly and promising alternative to the existing carbon sequestration methods. Nonetheless, the inherent relatively low photosynthetic capacity of microalgae has hampered the practical use of this strategy for CO2 biomitigation applications. Read More

    Cellulase hyper-production by Trichoderma reesei mutant SEU-7 on lactose.
    Biotechnol Biofuels 2017 4;10:228. Epub 2017 Oct 4.
    Department of Chemistry, University of Michigan, 930 North University Avenue, Ann Arbor, MI 48109 USA.
    Background: The induction of cellulase production by insoluble carbon source cellulose was a common and efficient strategy, but has some drawbacks, such as difficult fermentation operation, substantial cellulase loss, long fermentation time, and high energy-consumption, resulting in high cost of cellulase production in industry. These drawbacks can be overcome if soluble carbon sources are utilized as the inducers for cellulase production. However, until now the induction efficiency of most soluble carbon sources, especially lactose and glucose, is still inferior to cellulose despite extensive efforts have been made by either optimizing the fermentation process or constructing the recombinant strains. Read More

    Variation across a wheat genetic diversity panel for saccharification of hydrothermally pretreated straw.
    Biotechnol Biofuels 2017 2;10:227. Epub 2017 Oct 2.
    The Biorefinery Centre, Quadram Institute Bioscience, Norwich Research Park, Colney, Norwich, NR4 7UA UK.
    Background: Wheat straw forms an important, reliable source of lignocellulosic biomass for use in second-generation ethanol production. However, there is limited understanding of the variation in quality of straw from current breeding cultivars, and studies on such variation have generally employed suboptimal pretreatments. There is also a degree of confusion regarding phenotypic characteristics relevant to optimising the enzymatic saccharification of cellulose after suitable pretreatments for biorefining compared with those which determine good ruminant digestibility. Read More

    Co-production of 11α-hydroxyprogesterone and ethanol using recombinant yeast expressing fungal steroid hydroxylases.
    Biotechnol Biofuels 2017 29;10:226. Epub 2017 Sep 29.
    Institute of Life Science, Swansea University Medical School, Swansea, SA2 8PP Wales UK.
    Background: Bioethanol production from sustainable sources of biomass that limit effect on food production are needed and in a biorefinery approach co-products are desirable, obtained from both the plant material and from the microbial biomass. Fungal biotransformation of steroids was among the first industrial biotransformations allowing corticosteroid production. In this work, the potential of yeast to produce intermediates needed in corticosteroid production is demonstrated at laboratory scale following bioethanol production from perennial ryegrass juice. Read More

    Network reconstruction and systems analysis of plant cell wall deconstruction by Neurospora crassa.
    Biotechnol Biofuels 2017 21;10:225. Epub 2017 Sep 21.
    Energy Biosciences Institute, University of California Berkeley, Berkeley, CA 94704 USA.
    Background: Plant biomass degradation by fungal-derived enzymes is rapidly expanding in economic importance as a clean and efficient source for biofuels. The ability to rationally engineer filamentous fungi would facilitate biotechnological applications for degradation of plant cell wall polysaccharides. However, incomplete knowledge of biomolecular networks responsible for plant cell wall deconstruction impedes experimental efforts in this direction. Read More

    The effects of CO2 and H2 on CO metabolism by pure and mixed microbial cultures.
    Biotechnol Biofuels 2017 16;10:220. Epub 2017 Sep 16.
    Biodesign Swette Center for Environmental Biotechnology, Arizona State University, P.O. Box 875701, Tempe, AZ 85287-5701 USA.
    Background: Syngas fermentation, the bioconversion of CO, CO2, and H2 to biofuels and chemicals, has undergone considerable optimization for industrial applications. Even more, full-scale plants for ethanol production from syngas fermentation by pure cultures are being built worldwide. The composition of syngas depends on the feedstock gasified and the gasification conditions. Read More

    Removal of glucuronic acid from xylan is a strategy to improve the conversion of plant biomass to sugars for bioenergy.
    Biotechnol Biofuels 2017 19;10:224. Epub 2017 Sep 19.
    Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QW UK.
    Background: Plant lignocellulosic biomass can be a source of fermentable sugars for the production of second generation biofuels and biochemicals. The recalcitrance of this plant material is one of the major obstacles in its conversion into sugars. Biomass is primarily composed of secondary cell walls, which is made of cellulose, hemicelluloses and lignin. Read More

    High-level expression and characterization of a novel cutinase from Malbranchea cinnamomea suitable for butyl butyrate production.
    Biotechnol Biofuels 2017 19;10:223. Epub 2017 Sep 19.
    Beijing Advanced Innovation Center for Food Nutrition and Human Health, China Agricultural University, Beijing, 100083 China.
    Background: Butyl butyrate has been considered as a promising fuel source because it is a kind of natural ester which can be converted from renewable and sustainable lignocellulosic biomass. Compared with the conventional chemical methods for butyl butyrate production, the enzymatic approach has been demonstrated to be more attractive, mainly owing to the mild reaction conditions, high specificity, low energy consumption, and environmental friendliness. Cutinases play an important role in the butyl butyrate production process. Read More

    How does cellulosome composition influence deconstruction of lignocellulosic substrates in Clostridium (Ruminiclostridium) thermocellum DSM 1313?
    Biotechnol Biofuels 2017 18;10:222. Epub 2017 Sep 18.
    Department of Biomolecular Sciences, The Weizmann Institute of Science, 76100 Rehovot, Israel.
    Background: Bioethanol production processes involve enzymatic hydrolysis of pretreated lignocellulosic biomass into fermentable sugars. Due to the relatively high cost of enzyme production, the development of potent and cost-effective cellulolytic cocktails is critical for increasing the cost-effectiveness of bioethanol production. In this context, the multi-protein cellulolytic complex of Clostridium (Ruminiclostridium) thermocellum, the cellulosome, was studied here. Read More

    AtCesA8-driven OsSUS3 expression leads to largely enhanced biomass saccharification and lodging resistance by distinctively altering lignocellulose features in rice.
    Biotechnol Biofuels 2017 16;10:221. Epub 2017 Sep 16.
    Biomass and Bioenergy Research Centre, Huazhong Agricultural University, Wuhan, China.
    Background: Biomass recalcitrance and plant lodging are two complex traits that tightly associate with plant cell wall structure and features. Although genetic modification of plant cell walls can potentially reduce recalcitrance for enhancing biomass saccharification, it remains a challenge to maintain a normal growth with enhanced biomass yield and lodging resistance in transgenic plants. Sucrose synthase (SUS) is a key enzyme to regulate carbon partitioning by providing UDP-glucose as substrate for cellulose and other polysaccharide biosynthesis. Read More

    Determinants of stakeholders' attitudes towards biodiesel.
    Biotechnol Biofuels 2017 15;10:219. Epub 2017 Sep 15.
    Pusat Citra Universiti, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor Darul Ehsan Malaysia.
    Background: Concern about the inevitable depletion of global energy resources is rising and many countries are shifting their focus to renewable energy. Biodiesel is one promising energy source that has garnered much public attention in recent years. Many believe that this alternative source of energy will be able to sustain the need for increased energy security while at the same time being friendly to the environment. Read More

    A novel and efficient fungal delignification strategy based on versatile peroxidase for lignocellulose bioconversion.
    Biotechnol Biofuels 2017 13;10:218. Epub 2017 Sep 13.
    College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074 People's Republic of China.
    Background: The selective lignin-degrading white-rot fungi are regarded to be the best lignin degraders and have been widely used for reducing the saccharification recalcitrance of lignocellulose. However, the biological delignification and conversion of lignocellulose in biorefinery is still limited. It is necessary to develop novel and more efficient bio-delignification systems. Read More

    Greenhouse gas performance of biochemical biodiesel production from straw: soil organic carbon changes and time-dependent climate impact.
    Biotechnol Biofuels 2017 13;10:217. Epub 2017 Sep 13.
    Department of Energy and Technology, Swedish University of Agricultural Sciences, Uppsala, Sweden.
    Background: Use of bio-based diesel is increasing in Europe. It is currently produced from oilseed crops, but can also be generated from lignocellulosic biomass such as straw. However, removing straw affects soil organic carbon (SOC), with potential consequences for the climate impact of the biofuel. Read More

    Phenotypic landscape of non-conventional yeast species for different stress tolerance traits desirable in bioethanol fermentation.
    Biotechnol Biofuels 2017 13;10:216. Epub 2017 Sep 13.
    Laboratory of Molecular Cell Biology, Institute of Botany and Microbiology, VIB Center of Microbiology, KU Leuven, Kasteelpark Arenberg 31, B-3001 Louvain, Belgium.
    Background: Non-conventional yeasts present a huge, yet barely exploited, resource of yeast biodiversity for industrial applications. This presents a great opportunity to explore alternative ethanol-fermenting yeasts that are more adapted to some of the stress factors present in the harsh environmental conditions in second-generation (2G) bioethanol fermentation. Extremely tolerant yeast species are interesting candidates to investigate the underlying tolerance mechanisms and to identify genes that when transferred to existing industrial strains could help to design more stress-tolerant cell factories. Read More

    The yeast Geotrichum candidum encodes functional lytic polysaccharide monooxygenases.
    Biotechnol Biofuels 2017 12;10:215. Epub 2017 Sep 12.
    INRA, Aix Marseille University BBF, Biodiversité et Biotechnologie Fongiques, 13288 Marseille, France.
    Background: Lytic polysaccharide monooxygenases (LPMOs) are a class of powerful oxidative enzymes that have revolutionized our understanding of lignocellulose degradation. Fungal LPMOs of the AA9 family target cellulose and hemicelluloses. AA9 LPMO-coding genes have been identified across a wide range of fungal saprotrophs (Ascomycotina, Basidiomycotina, etc. Read More

    Characterization of a heat-tolerant Chlorella sp. GD mutant with enhanced photosynthetic CO2 fixation efficiency and its implication as lactic acid fermentation feedstock.
    Biotechnol Biofuels 2017 12;10:214. Epub 2017 Sep 12.
    Department of Chemical Engineering, National Cheng-Kung University, Tainan, 70146 Taiwan.
    Background: Fermentative production of lactic acid from algae-based carbohydrates devoid of lignin has attracted great attention for its potential as a suitable alternative substrate compared to lignocellulosic biomass.

    Results: A Chlorella sp. GD mutant with enhanced thermo-tolerance was obtained by mutagenesis using N-methyl-N'-nitro-N-nitrosoguanidine to overcome outdoor high-temperature inhibition and it was used as a feedstock for fermentative lactic acid production. Read More

    Sustaining fermentation in high-gravity ethanol production by feeding yeast to a temperature-profiled multifeed simultaneous saccharification and co-fermentation of wheat straw.
    Biotechnol Biofuels 2017 12;10:213. Epub 2017 Sep 12.
    Division of Industrial Biotechnology, Department of Biology and Biological Engineering, Chalmers University of Technology, Gothenburg, Sweden.
    Background: Considerable progress is being made in ethanol production from lignocellulosic feedstocks by fermentation, but negative effects of inhibitors on fermenting microorganisms are still challenging. Feeding preadapted cells has shown positive effects by sustaining fermentation in high-gravity simultaneous saccharification and co-fermentation (SSCF). Loss of cell viability has been reported in several SSCF studies on different substrates and seems to be the main reason for the declining ethanol production toward the end of the process. Read More

    Perspectives for biocatalytic lignin utilization: cleaving 4-O-5 and Cα-Cβ bonds in dimeric lignin model compounds catalyzed by a promiscuous activity of tyrosinase.
    Biotechnol Biofuels 2017 11;10:212. Epub 2017 Sep 11.
    Clean Energy Research Center, Korea Institute of Science and Technology (KIST), Seoul, 02792 Republic of Korea.
    Background: In the biorefinery utilizing lignocellulosic biomasses, lignin decomposition to value-added phenolic derivatives is a key issue, and recently biocatalytic delignification is emerging owing to its superior selectivity, low energy consumption, and unparalleled sustainability. However, besides heme-containing peroxidases and laccases, information about lignolytic biocatalysts is still limited till date.

    Results: Herein, we report a promiscuous activity of tyrosinase which is closely associated with delignification requiring high redox potentials (>1. Read More

    Unique organization and unprecedented diversity of the Bacteroides (Pseudobacteroides) cellulosolvens cellulosome system.
    Biotechnol Biofuels 2017 7;10:211. Epub 2017 Sep 7.
    Department of Biomolecular Sciences, Weizmann Institute of Science, Rehovot, Israel.
    Background: (Pseudo) Bacteroides cellulosolvens is an anaerobic, mesophilic, cellulolytic, cellulosome-producing clostridial bacterium capable of utilizing cellulose and cellobiose as carbon sources. Recently, we sequenced the B. cellulosolvens genome, and subsequent comprehensive bioinformatic analysis, herein reported, revealed an unprecedented number of cellulosome-related components, including 78 cohesin modules scattered among 31 scaffoldins and more than 200 dockerin-bearing ORFs. Read More

    Genome sequencing and transcriptome analysis of Trichoderma reesei QM9978 strain reveals a distal chromosome translocation to be responsible for loss of vib1 expression and loss of cellulase induction.
    Biotechnol Biofuels 2017 7;10:209. Epub 2017 Sep 7.
    IFP Energies Nouvelles, 1-4 Avenue de Bois-Préau, 92852 Rueil-Malmaison, France.
    Background: The hydrolysis of biomass to simple sugars used for the production of biofuels in biorefineries requires the action of cellulolytic enzyme mixtures. During the last 50 years, the ascomycete Trichoderma reesei, the main source of industrial cellulase and hemicellulase cocktails, has been subjected to several rounds of classical mutagenesis with the aim to obtain higher production levels. During these random genetic events, strains unable to produce cellulases were generated. Read More

    In vitro oxidative decarboxylation of free fatty acids to terminal alkenes by two new P450 peroxygenases.
    Biotechnol Biofuels 2017 7;10:208. Epub 2017 Sep 7.
    Shandong Provincial Key Laboratory of Synthetic Biology, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, No. 189 Songling Road, Qingdao, 266101 Shandong China.
    Background: P450 fatty acid decarboxylases represented by the unusual CYP152 peroxygenase family member OleTJE have been receiving great attention recently since these P450 enzymes are able to catalyze the simple and direct production of 1-alkenes for potential applications in biofuels and biomaterials. To gain more mechanistic insights, broader substrate spectra, and improved decarboxylative activities, it is demanded to discover and investigate more P450 fatty acid decarboxylases.

    Results: Here, we describe for the first time the expression, purification, and in vitro biochemical characterization of two new CYP152 peroxygenases, CYP-Aa162 and CYP-Sm46Δ29, that are capable of decarboxylating straight-chain saturated fatty acids. Read More

    Bacillus coagulans MA-13: a promising thermophilic and cellulolytic strain for the production of lactic acid from lignocellulosic hydrolysate.
    Biotechnol Biofuels 2017 7;10:210. Epub 2017 Sep 7.
    Dipartimento di Biologia, Università degli Studi di Napoli Federico II, Naples, Italy.
    Background: The transition from a petroleum-based economy towards more sustainable bioprocesses for the production of fuels and chemicals (circular economy) is necessary to alleviate the impact of anthropic activities on the global ecosystem. Lignocellulosic biomass-derived sugars are suitable alternative feedstocks that can be fermented or biochemically converted to value-added products. An example is lactic acid, which is an essential chemical for the production of polylactic acid, a biodegradable bioplastic. Read More

    Coexistence and competition of sulfate-reducing and methanogenic populations in an anaerobic hexadecane-degrading culture.
    Biotechnol Biofuels 2017 5;10:207. Epub 2017 Sep 5.
    Key Laboratory of Development and Application of Rural Renewable Energy, Biogas Institute of Ministry of Agriculture, Section 4-13, Renmin South Road, Chengdu, 610041 People's Republic of China.
    Background: Over three-fifths of the world's known crude oil cannot be recovered using state-of-the-art techniques, but microbial conversion of petroleum hydrocarbons trapped in oil reservoirs to methane is one promising path to increase the recovery of fossil fuels. The process requires cooperation between syntrophic bacteria and methanogenic archaea, which can be affected by sulfate-reducing prokaryotes (SRPs). However, the effects of sulfate on hydrocarbon degradation and methane production remain elusive, and the microbial communities involved are not well understood. Read More

    High-throughput analysis of chemical components and theoretical ethanol yield of dedicated bioenergy sorghum using dual-optimized partial least squares calibration models.
    Biotechnol Biofuels 2017 4;10:206. Epub 2017 Sep 4.
    College of Agronomy and Biotechnology, China Agricultural University, Beijing, 100193 China.
    Background: Due to its chemical composition and abundance, lignocellulosic biomass is an attractive feedstock source for global bioenergy production. However, chemical composition variations interfere with the success of any single methodology for efficient bioenergy extraction from diverse lignocellulosic biomass sources. Although chemical component distributions could guide process design, they are difficult to obtain and vary widely among lignocellulosic biomass types. Read More

    Saccharomyces cerevisiae strain comparison in glucose-xylose fermentations on defined substrates and in high-gravity SSCF: convergence in strain performance despite differences in genetic and evolutionary engineering history.
    Biotechnol Biofuels 2017 4;10:205. Epub 2017 Sep 4.
    Institute of Biotechnology and Biochemical Engineering, Graz University of Technology, Graz, Austria.
    Background: The most advanced strains of xylose-fermenting Saccharomyces cerevisiae still utilize xylose far less efficiently than glucose, despite the extensive metabolic and evolutionary engineering applied in their development. Systematic comparison of strains across literature is difficult due to widely varying conditions used for determining key physiological parameters. Here, we evaluate an industrial and a laboratory S. Read More

    Thermotolerant genes essential for survival at a critical high temperature in thermotolerant ethanologenic Zymomonas mobilis TISTR 548.
    Biotechnol Biofuels 2017 24;10:204. Epub 2017 Aug 24.
    Life Science, Graduate School of Science and Technology for Innovation, Yamaguchi University, Ube, 755-8505 Japan.
    Background: High-temperature fermentation (HTF) technology is expected to reduce the cost of bioconversion of biomass to fuels or chemicals. For stable HTF, the development of a thermotolerant microbe is indispensable. Elucidation of the molecular mechanism of thermotolerance would enable the thermal stability of microbes to be improved. Read More

    Screening and evolution of a novel protist xylose isomerase from the termite Reticulitermes speratus for efficient xylose fermentation in Saccharomyces cerevisiae.
    Biotechnol Biofuels 2017 23;10:203. Epub 2017 Aug 23.
    Bioinspired Systems Research-Domain, Toyota Central R&D Labs., Inc., 41-1, Yokomichi, Nagakute, Aichi 480-1192 Japan.
    Background: The yeast Saccharomyces cerevisiae, a promising host for lignocellulosic bioethanol production, is unable to metabolize xylose. In attempts to confer xylose utilization ability in S. cerevisiae, a number of xylose isomerase (XI) genes have been expressed heterologously in this yeast. Read More

    Single cell oil production by Trichosporon cutaneum from steam-exploded corn stover and its upgradation for production of long-chain α,ω-dicarboxylic acids.
    Biotechnol Biofuels 2017 23;10:202. Epub 2017 Aug 23.
    College of Life Sciences, Northwest A&F University, 22 Xinong Road, Yangling, 712100 Shaanxi China.
    Background: Single cell oil (SCO) production from lignocelluloses by oleaginous microorganisms is still high in production cost, making the subsequent production of biofuels inviable economically in such an era of low oil prices. Therefore, how to upgrade the final products of lignocellulose-based bioprocess to more valuable ones is becoming a more and more important issue.

    Results: Differently sourced cellulases were compared in the enzymatic hydrolysis of the steam-exploded corn stover (SECS) and the cellulase from the mixed culture of Trichoderma reesei and Aspergillus niger was found to have the highest enzymatic hydrolysis yield 86. Read More

    Enhanced polyhydroxyalkanoate (PHA) production from the organic fraction of municipal solid waste by using mixed microbial culture.
    Biotechnol Biofuels 2017 22;10:201. Epub 2017 Aug 22.
    Gruppo Ricicla labs-DiSAA-Università degli Studi di Milano, Via Celoria 2, 20133 Milan, Italy.
    Background: In Europe, almost 87.6 million tonnes of food waste are produced. Despite the high biological value of food waste, traditional management solutions do not consider it as a precious resource. Read More

    Metagenomic mining pectinolytic microbes and enzymes from an apple pomace-adapted compost microbial community.
    Biotechnol Biofuels 2017 22;10:198. Epub 2017 Aug 22.
    College of Food Science and Engineering, Northwest A&F University, Yangling, Shaanxi Province China.
    Background: Degradation of pectin in lignocellulosic materials is one of the key steps for biofuel production. Biological hydrolysis of pectin, i.e. Read More

    Propionic acid production from corn stover hydrolysate by Propionibacterium acidipropionici.
    Biotechnol Biofuels 2017 17;10:200. Epub 2017 Aug 17.
    National Bioenergy Center, National Renewable Energy Laboratory, Golden, CO 80401 USA.
    Background: The production of value-added chemicals alongside biofuels from lignocellulosic hydrolysates is critical for developing economically viable biorefineries. Here, the production of propionic acid (PA), a potential building block for C3-based chemicals, from corn stover hydrolysate is investigated using the native PA-producing bacterium Propionibacterium acidipropionici.

    Results: A wide range of culture conditions and process parameters were examined and experimentally optimized to maximize titer, rate, and yield of PA. Read More

    Anodic electro-fermentation of 3-hydroxypropionic acid from glycerol by recombinant Klebsiella pneumoniae L17 in a bioelectrochemical system.
    Biotechnol Biofuels 2017 17;10:199. Epub 2017 Aug 17.
    School of Chemical and Biomolecular Engineering, Pusan National University, Busan, 609-735 Republic of Korea.
    Background: 3-Hydroxypropionic acid (3-HP) is an important platform chemical which can be produced biologically from glycerol. Klebsiella pneumoniae is an ideal biocatalyst for 3-HP because it can grow well on glycerol and naturally synthesize the essential coenzyme B12. On the other hand, if higher yields and titers of 3-HP are to be achieved, the sustained regeneration of NAD(+) under anaerobic conditions, where coenzyme B12 is synthesized sustainably, is required. Read More

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