In vitro and in vivo characterization of threeglycoside hydrolase family 5 members reveals potent xyloglucan backbone-cleaving functions.
- Mohamed A Attia,
- Cassandra E Nelson,
- Wendy A Offen,
- Namrata Jain,
- Gideon J Davies,
- Jeffrey G Gardner,
- Harry Brumer
Biotechnol Biofuels 2018 17;11:45. Epub 2018 Feb 17.
1Michael Smith Laboratories, University of British Columbia, 2185 East Mall, Vancouver, BC V6T 1Z4 Canada.
Background: Xyloglucan (XyG) is a ubiquitous and fundamental polysaccharide of plant cell walls. Due to its structural complexity, XyG requires a combination of backbone-cleaving and sidechain-debranching enzymes for complete deconstruction into its component monosaccharides. The soil saprophytehas emerged as a genetically tractable model system to study biomass saccharification, in part due to its innate capacity to utilize a wide range of plant polysaccharides for growth. Read More