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    1308 results match your criteria Biotechnology for Biofuels [Journal]

    1 OF 27

    The effects of CO2 and H2 on CO metabolism by pure and mixed microbial cultures.
    Biotechnol Biofuels 2017 16;10:220. Epub 2017 Sep 16.
    Biodesign Swette Center for Environmental Biotechnology, Arizona State University, P.O. Box 875701, Tempe, AZ 85287-5701 USA.
    Background: Syngas fermentation, the bioconversion of CO, CO2, and H2 to biofuels and chemicals, has undergone considerable optimization for industrial applications. Even more, full-scale plants for ethanol production from syngas fermentation by pure cultures are being built worldwide. The composition of syngas depends on the feedstock gasified and the gasification conditions. Read More

    Removal of glucuronic acid from xylan is a strategy to improve the conversion of plant biomass to sugars for bioenergy.
    Biotechnol Biofuels 2017 19;10:224. Epub 2017 Sep 19.
    Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QW UK.
    Background: Plant lignocellulosic biomass can be a source of fermentable sugars for the production of second generation biofuels and biochemicals. The recalcitrance of this plant material is one of the major obstacles in its conversion into sugars. Biomass is primarily composed of secondary cell walls, which is made of cellulose, hemicelluloses and lignin. Read More

    High-level expression and characterization of a novel cutinase from Malbranchea cinnamomea suitable for butyl butyrate production.
    Biotechnol Biofuels 2017 19;10:223. Epub 2017 Sep 19.
    Beijing Advanced Innovation Center for Food Nutrition and Human Health, China Agricultural University, Beijing, 100083 China.
    Background: Butyl butyrate has been considered as a promising fuel source because it is a kind of natural ester which can be converted from renewable and sustainable lignocellulosic biomass. Compared with the conventional chemical methods for butyl butyrate production, the enzymatic approach has been demonstrated to be more attractive, mainly owing to the mild reaction conditions, high specificity, low energy consumption, and environmental friendliness. Cutinases play an important role in the butyl butyrate production process. Read More

    How does cellulosome composition influence deconstruction of lignocellulosic substrates in Clostridium (Ruminiclostridium) thermocellum DSM 1313?
    Biotechnol Biofuels 2017 18;10:222. Epub 2017 Sep 18.
    Department of Biomolecular Sciences, The Weizmann Institute of Science, 76100 Rehovot, Israel.
    Background: Bioethanol production processes involve enzymatic hydrolysis of pretreated lignocellulosic biomass into fermentable sugars. Due to the relatively high cost of enzyme production, the development of potent and cost-effective cellulolytic cocktails is critical for increasing the cost-effectiveness of bioethanol production. In this context, the multi-protein cellulolytic complex of Clostridium (Ruminiclostridium) thermocellum, the cellulosome, was studied here. Read More

    AtCesA8-driven OsSUS3 expression leads to largely enhanced biomass saccharification and lodging resistance by distinctively altering lignocellulose features in rice.
    Biotechnol Biofuels 2017 16;10:221. Epub 2017 Sep 16.
    Biomass and Bioenergy Research Centre, Huazhong Agricultural University, Wuhan, China.
    Background: Biomass recalcitrance and plant lodging are two complex traits that tightly associate with plant cell wall structure and features. Although genetic modification of plant cell walls can potentially reduce recalcitrance for enhancing biomass saccharification, it remains a challenge to maintain a normal growth with enhanced biomass yield and lodging resistance in transgenic plants. Sucrose synthase (SUS) is a key enzyme to regulate carbon partitioning by providing UDP-glucose as substrate for cellulose and other polysaccharide biosynthesis. Read More

    Determinants of stakeholders' attitudes towards biodiesel.
    Biotechnol Biofuels 2017 15;10:219. Epub 2017 Sep 15.
    Pusat Citra Universiti, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor Darul Ehsan Malaysia.
    Background: Concern about the inevitable depletion of global energy resources is rising and many countries are shifting their focus to renewable energy. Biodiesel is one promising energy source that has garnered much public attention in recent years. Many believe that this alternative source of energy will be able to sustain the need for increased energy security while at the same time being friendly to the environment. Read More

    A novel and efficient fungal delignification strategy based on versatile peroxidase for lignocellulose bioconversion.
    Biotechnol Biofuels 2017 13;10:218. Epub 2017 Sep 13.
    College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074 People's Republic of China.
    Background: The selective lignin-degrading white-rot fungi are regarded to be the best lignin degraders and have been widely used for reducing the saccharification recalcitrance of lignocellulose. However, the biological delignification and conversion of lignocellulose in biorefinery is still limited. It is necessary to develop novel and more efficient bio-delignification systems. Read More

    Greenhouse gas performance of biochemical biodiesel production from straw: soil organic carbon changes and time-dependent climate impact.
    Biotechnol Biofuels 2017 13;10:217. Epub 2017 Sep 13.
    Department of Energy and Technology, Swedish University of Agricultural Sciences, Uppsala, Sweden.
    Background: Use of bio-based diesel is increasing in Europe. It is currently produced from oilseed crops, but can also be generated from lignocellulosic biomass such as straw. However, removing straw affects soil organic carbon (SOC), with potential consequences for the climate impact of the biofuel. Read More

    Phenotypic landscape of non-conventional yeast species for different stress tolerance traits desirable in bioethanol fermentation.
    Biotechnol Biofuels 2017 13;10:216. Epub 2017 Sep 13.
    Laboratory of Molecular Cell Biology, Institute of Botany and Microbiology, VIB Center of Microbiology, KU Leuven, Kasteelpark Arenberg 31, B-3001 Louvain, Belgium.
    Background: Non-conventional yeasts present a huge, yet barely exploited, resource of yeast biodiversity for industrial applications. This presents a great opportunity to explore alternative ethanol-fermenting yeasts that are more adapted to some of the stress factors present in the harsh environmental conditions in second-generation (2G) bioethanol fermentation. Extremely tolerant yeast species are interesting candidates to investigate the underlying tolerance mechanisms and to identify genes that when transferred to existing industrial strains could help to design more stress-tolerant cell factories. Read More

    The yeast Geotrichum candidum encodes functional lytic polysaccharide monooxygenases.
    Biotechnol Biofuels 2017 12;10:215. Epub 2017 Sep 12.
    INRA, Aix Marseille University BBF, Biodiversité et Biotechnologie Fongiques, 13288 Marseille, France.
    Background: Lytic polysaccharide monooxygenases (LPMOs) are a class of powerful oxidative enzymes that have revolutionized our understanding of lignocellulose degradation. Fungal LPMOs of the AA9 family target cellulose and hemicelluloses. AA9 LPMO-coding genes have been identified across a wide range of fungal saprotrophs (Ascomycotina, Basidiomycotina, etc. Read More

    Characterization of a heat-tolerant Chlorella sp. GD mutant with enhanced photosynthetic CO2 fixation efficiency and its implication as lactic acid fermentation feedstock.
    Biotechnol Biofuels 2017 12;10:214. Epub 2017 Sep 12.
    Department of Chemical Engineering, National Cheng-Kung University, Tainan, 70146 Taiwan.
    Background: Fermentative production of lactic acid from algae-based carbohydrates devoid of lignin has attracted great attention for its potential as a suitable alternative substrate compared to lignocellulosic biomass.

    Results: A Chlorella sp. GD mutant with enhanced thermo-tolerance was obtained by mutagenesis using N-methyl-N'-nitro-N-nitrosoguanidine to overcome outdoor high-temperature inhibition and it was used as a feedstock for fermentative lactic acid production. Read More

    Sustaining fermentation in high-gravity ethanol production by feeding yeast to a temperature-profiled multifeed simultaneous saccharification and co-fermentation of wheat straw.
    Biotechnol Biofuels 2017 12;10:213. Epub 2017 Sep 12.
    Division of Industrial Biotechnology, Department of Biology and Biological Engineering, Chalmers University of Technology, Gothenburg, Sweden.
    Background: Considerable progress is being made in ethanol production from lignocellulosic feedstocks by fermentation, but negative effects of inhibitors on fermenting microorganisms are still challenging. Feeding preadapted cells has shown positive effects by sustaining fermentation in high-gravity simultaneous saccharification and co-fermentation (SSCF). Loss of cell viability has been reported in several SSCF studies on different substrates and seems to be the main reason for the declining ethanol production toward the end of the process. Read More

    Perspectives for biocatalytic lignin utilization: cleaving 4-O-5 and Cα-Cβ bonds in dimeric lignin model compounds catalyzed by a promiscuous activity of tyrosinase.
    Biotechnol Biofuels 2017 11;10:212. Epub 2017 Sep 11.
    Clean Energy Research Center, Korea Institute of Science and Technology (KIST), Seoul, 02792 Republic of Korea.
    Background: In the biorefinery utilizing lignocellulosic biomasses, lignin decomposition to value-added phenolic derivatives is a key issue, and recently biocatalytic delignification is emerging owing to its superior selectivity, low energy consumption, and unparalleled sustainability. However, besides heme-containing peroxidases and laccases, information about lignolytic biocatalysts is still limited till date.

    Results: Herein, we report a promiscuous activity of tyrosinase which is closely associated with delignification requiring high redox potentials (>1. Read More

    Unique organization and unprecedented diversity of the Bacteroides (Pseudobacteroides) cellulosolvens cellulosome system.
    Biotechnol Biofuels 2017 7;10:211. Epub 2017 Sep 7.
    Department of Biomolecular Sciences, Weizmann Institute of Science, Rehovot, Israel.
    Background: (Pseudo) Bacteroides cellulosolvens is an anaerobic, mesophilic, cellulolytic, cellulosome-producing clostridial bacterium capable of utilizing cellulose and cellobiose as carbon sources. Recently, we sequenced the B. cellulosolvens genome, and subsequent comprehensive bioinformatic analysis, herein reported, revealed an unprecedented number of cellulosome-related components, including 78 cohesin modules scattered among 31 scaffoldins and more than 200 dockerin-bearing ORFs. Read More

    Genome sequencing and transcriptome analysis of Trichoderma reesei QM9978 strain reveals a distal chromosome translocation to be responsible for loss of vib1 expression and loss of cellulase induction.
    Biotechnol Biofuels 2017 7;10:209. Epub 2017 Sep 7.
    IFP Energies Nouvelles, 1-4 Avenue de Bois-Préau, 92852 Rueil-Malmaison, France.
    Background: The hydrolysis of biomass to simple sugars used for the production of biofuels in biorefineries requires the action of cellulolytic enzyme mixtures. During the last 50 years, the ascomycete Trichoderma reesei, the main source of industrial cellulase and hemicellulase cocktails, has been subjected to several rounds of classical mutagenesis with the aim to obtain higher production levels. During these random genetic events, strains unable to produce cellulases were generated. Read More

    In vitro oxidative decarboxylation of free fatty acids to terminal alkenes by two new P450 peroxygenases.
    Biotechnol Biofuels 2017 7;10:208. Epub 2017 Sep 7.
    Shandong Provincial Key Laboratory of Synthetic Biology, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, No. 189 Songling Road, Qingdao, 266101 Shandong China.
    Background: P450 fatty acid decarboxylases represented by the unusual CYP152 peroxygenase family member OleTJE have been receiving great attention recently since these P450 enzymes are able to catalyze the simple and direct production of 1-alkenes for potential applications in biofuels and biomaterials. To gain more mechanistic insights, broader substrate spectra, and improved decarboxylative activities, it is demanded to discover and investigate more P450 fatty acid decarboxylases.

    Results: Here, we describe for the first time the expression, purification, and in vitro biochemical characterization of two new CYP152 peroxygenases, CYP-Aa162 and CYP-Sm46Δ29, that are capable of decarboxylating straight-chain saturated fatty acids. Read More

    Bacillus coagulans MA-13: a promising thermophilic and cellulolytic strain for the production of lactic acid from lignocellulosic hydrolysate.
    Biotechnol Biofuels 2017 7;10:210. Epub 2017 Sep 7.
    Dipartimento di Biologia, Università degli Studi di Napoli Federico II, Naples, Italy.
    Background: The transition from a petroleum-based economy towards more sustainable bioprocesses for the production of fuels and chemicals (circular economy) is necessary to alleviate the impact of anthropic activities on the global ecosystem. Lignocellulosic biomass-derived sugars are suitable alternative feedstocks that can be fermented or biochemically converted to value-added products. An example is lactic acid, which is an essential chemical for the production of polylactic acid, a biodegradable bioplastic. Read More

    Coexistence and competition of sulfate-reducing and methanogenic populations in an anaerobic hexadecane-degrading culture.
    Biotechnol Biofuels 2017 5;10:207. Epub 2017 Sep 5.
    Key Laboratory of Development and Application of Rural Renewable Energy, Biogas Institute of Ministry of Agriculture, Section 4-13, Renmin South Road, Chengdu, 610041 People's Republic of China.
    Background: Over three-fifths of the world's known crude oil cannot be recovered using state-of-the-art techniques, but microbial conversion of petroleum hydrocarbons trapped in oil reservoirs to methane is one promising path to increase the recovery of fossil fuels. The process requires cooperation between syntrophic bacteria and methanogenic archaea, which can be affected by sulfate-reducing prokaryotes (SRPs). However, the effects of sulfate on hydrocarbon degradation and methane production remain elusive, and the microbial communities involved are not well understood. Read More

    High-throughput analysis of chemical components and theoretical ethanol yield of dedicated bioenergy sorghum using dual-optimized partial least squares calibration models.
    Biotechnol Biofuels 2017 4;10:206. Epub 2017 Sep 4.
    College of Agronomy and Biotechnology, China Agricultural University, Beijing, 100193 China.
    Background: Due to its chemical composition and abundance, lignocellulosic biomass is an attractive feedstock source for global bioenergy production. However, chemical composition variations interfere with the success of any single methodology for efficient bioenergy extraction from diverse lignocellulosic biomass sources. Although chemical component distributions could guide process design, they are difficult to obtain and vary widely among lignocellulosic biomass types. Read More

    Saccharomyces cerevisiae strain comparison in glucose-xylose fermentations on defined substrates and in high-gravity SSCF: convergence in strain performance despite differences in genetic and evolutionary engineering history.
    Biotechnol Biofuels 2017 4;10:205. Epub 2017 Sep 4.
    Institute of Biotechnology and Biochemical Engineering, Graz University of Technology, Graz, Austria.
    Background: The most advanced strains of xylose-fermenting Saccharomyces cerevisiae still utilize xylose far less efficiently than glucose, despite the extensive metabolic and evolutionary engineering applied in their development. Systematic comparison of strains across literature is difficult due to widely varying conditions used for determining key physiological parameters. Here, we evaluate an industrial and a laboratory S. Read More

    Thermotolerant genes essential for survival at a critical high temperature in thermotolerant ethanologenic Zymomonas mobilis TISTR 548.
    Biotechnol Biofuels 2017 24;10:204. Epub 2017 Aug 24.
    Life Science, Graduate School of Science and Technology for Innovation, Yamaguchi University, Ube, 755-8505 Japan.
    Background: High-temperature fermentation (HTF) technology is expected to reduce the cost of bioconversion of biomass to fuels or chemicals. For stable HTF, the development of a thermotolerant microbe is indispensable. Elucidation of the molecular mechanism of thermotolerance would enable the thermal stability of microbes to be improved. Read More

    Screening and evolution of a novel protist xylose isomerase from the termite Reticulitermes speratus for efficient xylose fermentation in Saccharomyces cerevisiae.
    Biotechnol Biofuels 2017 23;10:203. Epub 2017 Aug 23.
    Bioinspired Systems Research-Domain, Toyota Central R&D Labs., Inc., 41-1, Yokomichi, Nagakute, Aichi 480-1192 Japan.
    Background: The yeast Saccharomyces cerevisiae, a promising host for lignocellulosic bioethanol production, is unable to metabolize xylose. In attempts to confer xylose utilization ability in S. cerevisiae, a number of xylose isomerase (XI) genes have been expressed heterologously in this yeast. Read More

    Single cell oil production by Trichosporon cutaneum from steam-exploded corn stover and its upgradation for production of long-chain α,ω-dicarboxylic acids.
    Biotechnol Biofuels 2017 23;10:202. Epub 2017 Aug 23.
    College of Life Sciences, Northwest A&F University, 22 Xinong Road, Yangling, 712100 Shaanxi China.
    Background: Single cell oil (SCO) production from lignocelluloses by oleaginous microorganisms is still high in production cost, making the subsequent production of biofuels inviable economically in such an era of low oil prices. Therefore, how to upgrade the final products of lignocellulose-based bioprocess to more valuable ones is becoming a more and more important issue.

    Results: Differently sourced cellulases were compared in the enzymatic hydrolysis of the steam-exploded corn stover (SECS) and the cellulase from the mixed culture of Trichoderma reesei and Aspergillus niger was found to have the highest enzymatic hydrolysis yield 86. Read More

    Enhanced polyhydroxyalkanoate (PHA) production from the organic fraction of municipal solid waste by using mixed microbial culture.
    Biotechnol Biofuels 2017 22;10:201. Epub 2017 Aug 22.
    Gruppo Ricicla labs-DiSAA-Università degli Studi di Milano, Via Celoria 2, 20133 Milan, Italy.
    Background: In Europe, almost 87.6 million tonnes of food waste are produced. Despite the high biological value of food waste, traditional management solutions do not consider it as a precious resource. Read More

    Metagenomic mining pectinolytic microbes and enzymes from an apple pomace-adapted compost microbial community.
    Biotechnol Biofuels 2017 22;10:198. Epub 2017 Aug 22.
    College of Food Science and Engineering, Northwest A&F University, Yangling, Shaanxi Province China.
    Background: Degradation of pectin in lignocellulosic materials is one of the key steps for biofuel production. Biological hydrolysis of pectin, i.e. Read More

    Propionic acid production from corn stover hydrolysate by Propionibacterium acidipropionici.
    Biotechnol Biofuels 2017 17;10:200. Epub 2017 Aug 17.
    National Bioenergy Center, National Renewable Energy Laboratory, Golden, CO 80401 USA.
    Background: The production of value-added chemicals alongside biofuels from lignocellulosic hydrolysates is critical for developing economically viable biorefineries. Here, the production of propionic acid (PA), a potential building block for C3-based chemicals, from corn stover hydrolysate is investigated using the native PA-producing bacterium Propionibacterium acidipropionici.

    Results: A wide range of culture conditions and process parameters were examined and experimentally optimized to maximize titer, rate, and yield of PA. Read More

    Anodic electro-fermentation of 3-hydroxypropionic acid from glycerol by recombinant Klebsiella pneumoniae L17 in a bioelectrochemical system.
    Biotechnol Biofuels 2017 17;10:199. Epub 2017 Aug 17.
    School of Chemical and Biomolecular Engineering, Pusan National University, Busan, 609-735 Republic of Korea.
    Background: 3-Hydroxypropionic acid (3-HP) is an important platform chemical which can be produced biologically from glycerol. Klebsiella pneumoniae is an ideal biocatalyst for 3-HP because it can grow well on glycerol and naturally synthesize the essential coenzyme B12. On the other hand, if higher yields and titers of 3-HP are to be achieved, the sustained regeneration of NAD(+) under anaerobic conditions, where coenzyme B12 is synthesized sustainably, is required. Read More

    Time-resolved transcriptome analysis and lipid pathway reconstruction of the oleaginous green microalga Monoraphidium neglectum reveal a model for triacylglycerol and lipid hyperaccumulation.
    Biotechnol Biofuels 2017 14;10:197. Epub 2017 Aug 14.
    Algae Biotechnology and Bioenergy, Faculty of Biology, Center for Biotechnology (CeBiTec), Bielefeld University, 33615 Bielefeld, Germany.
    Background: Oleaginous microalgae are promising production hosts for the sustainable generation of lipid-based bioproducts and as bioenergy carriers such as biodiesel. Transcriptomics of the lipid accumulation phase, triggered efficiently by nitrogen starvation, is a valuable approach for the identification of gene targets for metabolic engineering.

    Results: An explorative analysis of the detailed transcriptional response to different stages of nitrogen availability was performed in the oleaginous green alga Monoraphidium neglectum. Read More

    Photoautotrophic production of polyhydroxyalkanoates in a synthetic mixed culture of Synechococcus elongatus cscB and Pseudomonas putida cscAB.
    Biotechnol Biofuels 2017 19;10:190. Epub 2017 Jul 19.
    Fachgebiet für Systembiotechnologie, Technische Universität München, Boltzmannstr 15, 85748 Garching, Germany.
    Background: One of the major challenges for the present and future generations is to find suitable substitutes for the fossil resources we rely on today. Cyanobacterial carbohydrates have been discussed as an emerging renewable feedstock in industrial biotechnology for the production of fuels and chemicals, showing promising production rates when compared to crop-based feedstock. However, intrinsic capacities of cyanobacteria to produce biotechnological compounds are limited and yields are low. Read More

    Engineering Shewanella oneidensis enables xylose-fed microbial fuel cell.
    Biotechnol Biofuels 2017 8;10:196. Epub 2017 Aug 8.
    Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072 China.
    Background: The microbial fuel cell (MFC) is a green and sustainable technology for electricity energy harvest from biomass, in which exoelectrogens use metabolism and extracellular electron transfer pathways for the conversion of chemical energy into electricity. However, Shewanella oneidensis MR-1, one of the most well-known exoelectrogens, could not use xylose (a key pentose derived from hydrolysis of lignocellulosic biomass) for cell growth and power generation, which limited greatly its practical applications.

    Results: Herein, to enable S. Read More

    Bioethanol production from spent mushroom compost derived from chaff of millet and sorghum.
    Biotechnol Biofuels 2017 4;10:195. Epub 2017 Aug 4.
    The Biorefinery Centre, Quadram Institute Bioscience, Colney, Norwich Research Park, Norwich, NR4 7UA UK.
    Background: In Uganda, the chaff remaining from threshed panicles of millet and sorghum is a low value, lignocellulose-rich agricultural by-product. Currently, it is used as a substrate for the cultivation of edible Oyster mushrooms (Pleurotus ostreatus). The aim of this study was to assess the potential to exploit the residual post-harvest compost for saccharification and fermentation to produce ethanol. Read More

    A novel transcription factor specifically regulates GH11 xylanase genes in Trichoderma reesei.
    Biotechnol Biofuels 2017 3;10:194. Epub 2017 Aug 3.
    CAS-Key Laboratory of Synthetic Biology, CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Chinese Academy of Science, Fenglin Rd 300, Shanghai, 200032 China.
    Background: The filamentous fungus Trichoderma reesei is widely utilized in industry for cellulase production, but its xylanase activity must be improved to enhance the accessibility of lignocellulose to cellulases. Several transcription factors play important roles in this progress; however, nearly all the reported transcription factors typically target both cellulase and hemi-cellulase genes. Specific xylanase transcription factor would be useful to regulate xylanase activity directly. Read More

    Improving ethanol productivity through self-cycling fermentation of yeast: a proof of concept.
    Biotechnol Biofuels 2017 2;10:193. Epub 2017 Aug 2.
    Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, T6G 2P5 Canada.
    Background: The cellulosic ethanol industry has developed efficient strategies for converting sugars obtained from various cellulosic feedstocks to bioethanol. However, any further major improvements in ethanol productivity will require development of novel and innovative fermentation strategies that enhance incumbent technologies in a cost-effective manner. The present study investigates the feasibility of applying self-cycling fermentation (SCF) to cellulosic ethanol production to elevate productivity. Read More

    Lignin valorization: lignin nanoparticles as high-value bio-additive for multifunctional nanocomposites.
    Biotechnol Biofuels 2017 24;10:192. Epub 2017 Jul 24.
    State Key Laboratory of Polymer Materials Engineering, Polymer Research Institute of Sichuan University, Chengdu, 610065 China.
    Background: Although conversion of low value but high-volume lignin by-product to its usable form is one of the determinant factors for building an economically feasible integrated lignocellulose biorefinery, it has been challenged by its structural complexity and inhomogeneity. We and others have shown that uniform lignin nanoparticles can be produced from a wide range of technical lignins, despite the varied lignocellulosic biomass and the pretreatment methods/conditions applied. This value-added nanostructure lignin enriched with multifunctional groups can be a promising versatile material platform for various downstream utilizations especially in the emerging nanocomposite fields. Read More

    The impact of considering land intensification and updated data on biofuels land use change and emissions estimates.
    Biotechnol Biofuels 2017 20;10:191. Epub 2017 Jul 20.
    Department of Agricultural Economics, Purdue University, West Lafayette, USA.
    Background: The GTAP model has been used to estimate biofuel policy induced land use changes and consequent GHG emissions for more than a decade. This paper reviews the history of the model and database modifications and improvements that have occurred over that period. In particular, the paper covers in greater detail the move from the 2004 to the 2011 database, and the inclusion of cropland intensification in the modeling structure. Read More

    Genome-wide landscape of position effects on heterogeneous gene expression in Saccharomyces cerevisiae.
    Biotechnol Biofuels 2017 18;10:189. Epub 2017 Jul 18.
    Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072 People's Republic of China.
    Background: Integration of heterogeneous genes is widely applied in synthetic biology and metabolic engineering. However, knowledge about the effect of integrative position on gene expression remains limited.

    Results: We established a genome-wide landscape of position effect on gene expression in Saccharomyces cerevisiae. Read More

    Towards microalgal triglycerides in the commodity markets.
    Biotechnol Biofuels 2017 17;10:188. Epub 2017 Jul 17.
    Bioprocess Engineering, AlgaePARC, Wageningen University, P.O. Box 16, 6700 AA Wageningen, The Netherlands.
    Background: Microalgal triglycerides (TAGs) hold great promise as sustainable feedstock for commodity industries. However, to determine research priorities and support business decisions, solid techno-economic studies are essential. Here, we present a techno-economic analysis of two-step TAG production (growth reactors are operated in continuous mode such that multiple batch-operated stress reactors are inoculated and harvested sequentially) for a 100-ha plant in southern Spain using vertically stacked tubular photobioreactors. Read More

    Heterologous co-expression of a yeast diacylglycerol acyltransferase (ScDGA1) and a plant oleosin (AtOLEO3) as an efficient tool for enhancing triacylglycerol accumulation in the marine diatom Phaeodactylum tricornutum.
    Biotechnol Biofuels 2017 17;10:187. Epub 2017 Jul 17.
    Department of Plant Biochemistry, Albrecht-von-Haller-Institute for Plant Sciences, University of Goettingen, 37077 Goettingen, Germany.
    Background: Microalgae are promising alternate and renewable sources for producing valuable products such as biofuel and essential fatty acids. Although this is the case, there are still challenges impeding on the effective commercial production of microalgal products. For instance, their product yield is still too low. Read More

    Highly efficient methane generation from untreated microalgae biomass.
    Biotechnol Biofuels 2017 17;10:186. Epub 2017 Jul 17.
    Department of Biology/Center for Biotechnology (CeBiTec), Bielefeld University, Universitätsstrasse 27, 33615 Bielefeld, Germany.
    Background: The fact that microalgae perform very efficiently photosynthetic conversion of sunlight into chemical energy has moved them into the focus of regenerative fuel research. Especially, biogas generation via anaerobic digestion is economically attractive due to the comparably simple apparative process technology and the theoretical possibility of converting the entire algal biomass to biogas/methane. In the last 60 years, intensive research on biogas production from microalgae biomass has revealed the microalgae as a rather challenging substrate for anaerobic digestion due to its high cell wall recalcitrance and unfavorable protein content, which requires additional pretreatment and co-fermentation strategies for sufficient fermentation. Read More

    Two bifunctional enzymes from the marine protist Thraustochytrium roseum: biochemical characterization of wax ester synthase/acyl-CoA:diacylglycerol acyltransferase activity catalyzing wax ester and triacylglycerol synthesis.
    Biotechnol Biofuels 2017 15;10:185. Epub 2017 Jul 15.
    Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Wuhan, 430062 China.
    Background: Triacylglycerols (TAGs) and wax esters (WEs) are important neutral lipids which serve as energy reservoir in some plants and microorganisms. In recent years, these biologically produced neutral lipids have been regarded as potential alternative energy sources for biofuel production because of the increased interest on developing renewable and environmentally benign alternatives for fossil fuels. In bacteria, the final step in TAG and WE biosynthetic pathway is catalyzed by wax ester synthase/acyl coenzyme A (acyl-CoA):diacylglycerol acyltransferase (WS/DGAT). Read More

    Identification of developmental stage and anatomical fraction contributions to cell wall recalcitrance in switchgrass.
    Biotechnol Biofuels 2017 15;10:184. Epub 2017 Jul 15.
    Department of Chemical Engineering and Materials Science, Michigan State University, East Lansing, MI USA.
    Background: Heterogeneity within herbaceous biomass can present important challenges for processing feedstocks to cellulosic biofuels. Alterations to cell wall composition and organization during plant growth represent major contributions to heterogeneity within a single species or cultivar. To address this challenge, the focus of this study was to characterize the relationship between composition and properties of the plant cell wall and cell wall response to deconstruction by NaOH pretreatment and enzymatic hydrolysis for anatomical fractions (stem internodes, leaf sheaths, and leaf blades) within switchgrass at various tissue maturities as assessed by differing internode. Read More

    Modeling and simulation of the redox regulation of the metabolism in Escherichia coli at different oxygen concentrations.
    Biotechnol Biofuels 2017 14;10:183. Epub 2017 Jul 14.
    Department of Bioscience and Bioinformatics, Kyushu Institute of Technology, 680-4 Kawazu, Iizuka, Fukuoka, 820-8502 Japan.
    Background: Microbial production of biofuels and biochemicals from renewable feedstocks has received considerable recent attention from environmental protection and energy production perspectives. Many biofuels and biochemicals are produced by fermentation under oxygen-limited conditions following initiation of aerobic cultivation to enhance the cell growth rate. Thus, it is of significant interest to investigate the effect of dissolved oxygen concentration on redox regulation in Escherichia coli, a particularly popular cellular factory due to its high growth rate and well-characterized physiology. Read More

    Comparison of four glycosyl residue composition methods for effectiveness in detecting sugars from cell walls of dicot and grass tissues.
    Biotechnol Biofuels 2017 14;10:182. Epub 2017 Jul 14.
    Department of Biochemistry and Molecular Biology, University of Georgia, Athens, GA 30602 USA.
    Background: The effective use of plant biomass for biofuel and bioproduct production requires a comprehensive glycosyl residue composition analysis to understand the different cell wall polysaccharides present in the different biomass sources. Here we compared four methods side-by-side for their ability to measure the neutral and acidic sugar composition of cell walls from herbaceous, grass, and woody model plants and bioenergy feedstocks.

    Results: Arabidopsis, Populus, rice, and switchgrass leaf cell walls, as well as cell walls from Populus wood, rice stems, and switchgrass tillers, were analyzed by (1) gas chromatography-mass spectrometry (GC-MS) of alditol acetates combined with a total uronic acid assay; (2) carbodiimide reduction of uronic acids followed by GC-MS of alditol acetates; (3) GC-MS of trimethylsilyl (TMS) derivatives; and (4) high-pressure, anion-exchange chromatography (HPAEC). Read More

    Comparison of ethanol production from corn cobs and switchgrass following a pyrolysis-based biorefinery approach.
    Biotechnol Biofuels 2016 9;9:242. Epub 2016 Nov 9.
    Department of Chemical and Biochemical Engineering, University of Western Ontario, 1151 Richmond Street, London, ON Canada.
    Background: One of the main obstacles in lignocellulosic ethanol production is the necessity of pretreatment and fractionation of the biomass feedstocks to produce sufficiently pure fermentable carbohydrates. In addition, the by-products (hemicellulose and lignin fraction) are of low value, when compared to dried distillers grains (DDG), the main by-product of corn ethanol. Fast pyrolysis is an alternative thermal conversion technology for processing biomass. Read More

    Transcriptome and key genes expression related to carbon fixation pathways in Chlorella PY-ZU1 cells and their growth under high concentrations of CO2.
    Biotechnol Biofuels 2017 11;10:181. Epub 2017 Jul 11.
    State Key Laboratory of Clean Energy Utilization, Zhejiang University, Hangzhou, 310027 China.
    Background: The biomass yield of Chlorella PY-ZU1 drastically increased when cultivated under high CO2 condition compared with that cultivated under air condition. However, less attention has been given to the microalgae photosynthetic mechanisms response to different CO2 concentrations. The genetic reasons for the higher growth rate, CO2 fixation rate, and photosynthetic efficiency of microalgal cells under higher CO2 concentration have not been clearly defined yet. Read More

    Electricity-assisted production of caproic acid from grass.
    Biotechnol Biofuels 2017 11;10:180. Epub 2017 Jul 11.
    Department of Biochemical and Microbial Technology, Centre for Microbial Ecology and Technology (CMET), Ghent University, Coupure Links 653, 9000 Ghent, Belgium.
    Background: Medium chain carboxylic acids, such as caproic acid, are conventionally produced from food materials. Caproic acid can be produced through fermentation by the reverse β-oxidation of lactic acid, generated from low value lignocellulosic biomass. In situ extraction of caproic acid can be achieved by membrane electrolysis coupled to the fermentation process, allowing recovery by phase separation. Read More

    Modification of the nanostructure of lignocellulose cell walls via a non-enzymatic lignocellulose deconstruction system in brown rot wood-decay fungi.
    Biotechnol Biofuels 2017 11;10:179. Epub 2017 Jul 11.
    Biology and Soft Matter Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831 USA.
    Wood decayed by brown rot fungi and wood treated with the chelator-mediated Fenton (CMF) reaction, either alone or together with a cellulose enzyme cocktail, was analyzed by small angle neutron scattering (SANS), sum frequency generation (SFG) spectroscopy, Fourier transform infrared (FTIR) analysis, X-ray diffraction (XRD), atomic force microscopy (AFM), and transmission electron microscopy (TEM). Results showed that the CMF mechanism mimicked brown rot fungal attack for both holocellulose and lignin components of the wood. Crystalline cellulose and lignin were both depolymerized by the CMF reaction. Read More

    Metabolic engineering of Rhodopseudomonas palustris for the obligate reduction of n-butyrate to n-butanol.
    Biotechnol Biofuels 2017 11;10:178. Epub 2017 Jul 11.
    Department of Biological and Environmental Engineering, Cornell University, Ithaca, NY 14853 USA.
    Background: Rhodopseudomonas palustris is a versatile microbe that encounters an innate redox imbalance while growing photoheterotrophically with reduced substrates. The resulting excess in reducing equivalents, together with ATP from photosynthesis, could be utilized to drive a wide range of bioconversions. The objective of this study was to genetically modify R. Read More

    Enzymatic degradation of sulfite-pulped softwoods and the role of LPMOs.
    Biotechnol Biofuels 2017 11;10:177. Epub 2017 Jul 11.
    Faculty of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences, P.O. Box 5003, 1432 Ås, Norway.
    Background: Recent advances in the development of enzyme cocktails for degradation of lignocellulosic biomass, especially the discovery of lytic polysaccharide monooxygenases (LPMOs), have opened new perspectives for process design and optimization. Softwood biomass is an abundant resource in many parts of the world, including Scandinavia, but efficient pretreatment and subsequent enzymatic hydrolysis of softwoods are challenging. Sulfite pulping-based pretreatments, such as in the BALI™ process, yield substrates that are relatively easy to degrade. Read More

    Limitation of cellulose accessibility and unproductive binding of cellulases by pretreated sugarcane bagasse lignin.
    Biotechnol Biofuels 2017 11;10:176. Epub 2017 Jul 11.
    Departamento de Biotecnologia, Escola de Engenharia de Lorena, Universidade de São Paulo, CP 116, Lorena, SP 12602-810 Brazil.
    Background: The effectiveness of the enzymatic hydrolysis of cellulose in plant cell wall is strongly influenced by the access of enzymes to cellulose, which is at least in part limited by the presence of lignin. Although physicochemical treatments preceding the enzymatic catalysis significantly overcome this recalcitrance, the residual lignin can still play a role in the process. Lignin is suggested to act as a barrier, hindering cellulose and limiting the access of the enzymes. Read More

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