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    1440 results match your criteria Biotechnology for Biofuels [Journal]

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    In vitro and in vivo characterization of threeglycoside hydrolase family 5 members reveals potent xyloglucan backbone-cleaving functions.
    Biotechnol Biofuels 2018 17;11:45. Epub 2018 Feb 17.
    1Michael Smith Laboratories, University of British Columbia, 2185 East Mall, Vancouver, BC V6T 1Z4 Canada.
    Background: Xyloglucan (XyG) is a ubiquitous and fundamental polysaccharide of plant cell walls. Due to its structural complexity, XyG requires a combination of backbone-cleaving and sidechain-debranching enzymes for complete deconstruction into its component monosaccharides. The soil saprophytehas emerged as a genetically tractable model system to study biomass saccharification, in part due to its innate capacity to utilize a wide range of plant polysaccharides for growth. Read More

    Tension wood structure and morphology conducive for better enzymatic digestion.
    Biotechnol Biofuels 2018 16;11:44. Epub 2018 Feb 16.
    3Neutron Scattering Division, Oak Ridge National Laboratory, 1 Bethel Valley Road, P.O. Box 2008, Oak Ridge, TN 37831 USA.
    Background: Tension wood is a type of reaction wood in response to bending or leaning stem as a corrective growth process. Tension wood is formed by both natural and man-made processes. Most attractively, tension wood contains higher glucan content and undergoes higher enzymatic conversion to fermentable sugars. Read More

    Function analysis of 5'-UTR of the cellulosomal-cluster in.
    Biotechnol Biofuels 2018 16;11:43. Epub 2018 Feb 16.
    3Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Institute of Biotechnology, Shanxi University, Taiyuan, 030006 Shanxi Province China.
    Background: Anaerobic, mesophilic, and cellulolyticproduces an efficient cellulolytic extracellular complex named cellulosome that hydrolyzes plant cell wall polysaccharides into simple sugars. Its genome harbors two long cellulosomal clusters:-operon encoding major cellulosome components (including scaffolding) and-gene cluster encoding hemicellulases. Compared with works on-operon, there are much fewer studies on-mainly due to its rare location in cellulolytic clostridia. Read More

    Genomic comparison ofspecies with the potential of utilizing red algal biomass for biobutanol production.
    Biotechnol Biofuels 2018 15;11:42. Epub 2018 Feb 15.
    1Department of Biology, Shantou University, Shantou, 515063 Guangdong China.
    Background: Sustainable biofuels, which are widely considered as an attractive alternative to fossil fuels, can be generated by utilizing various biomass from the environment. Marine biomass, such as red algal biomass, is regarded as one potential renewable substrate source for biofuels conversion due to its abundance of fermentable sugars (e.g. Read More

    Real-time imaging reveals that lytic polysaccharide monooxygenase promotes cellulase activity by increasing cellulose accessibility.
    Biotechnol Biofuels 2018 15;11:41. Epub 2018 Feb 15.
    1Department of Plant Biology, Michigan State University, East Lansing, MI 48824 USA.
    Background: The high cost of enzymes is one of the key technical barriers that must be overcome to realize the economical production of biofuels and biomaterials from biomass. Supplementation of enzyme cocktails with lytic polysaccharide monooxygenase (LPMO) can increase the efficiency of these cellulase mixtures for biomass conversion. The previous studies have revealed that LPMOs cleave polysaccharide chains by oxidization of the C1 and/or C4 carbons of the monomeric units. Read More

    Growth and lipid accumulation by different nutrients in the microalga.
    Biotechnol Biofuels 2018 13;11:40. Epub 2018 Feb 13.
    1Shenzhen Key Laboratory of Marine Bioresource & Eco-environmental Science, Shenzhen Engineering Laboratory for Marine Algal Biotechnology, Guangdong Provincial Key Laboratory for Plant Epigenetics, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen, 518060 Guangdong People's Republic of China.
    Background: Individual nutrient depletion is widely used to induce lipid accumulation in microalgae, which also causes cell growth inhibition and decreases the total biomass. Thus, improving the lipid accumulation without biomass loss in the nutrient deficiency cells becomes a potential cost-effective treatment for cheaper biofuels.

    Methods: In this study, the effects of different nutritional conditions on the growth and contents of lipids inwere compared, and the metabolic profiles under different nutritional conditions were also investigated. Read More

    Molybdenum anode: a novel electrode for enhanced power generation in microbial fuel cells, identified via extensive screening of metal electrodes.
    Biotechnol Biofuels 2018 13;11:39. Epub 2018 Feb 13.
    Division of Animal Environment and Waste Management Research, Institute of Livestock and Grassland Science, National Agriculture and Food Research Organization (NARO), 2 Ikenodai, Tsukuba, 305-0901 Japan.
    Background: Metals are considered a suitable anode material for microbial fuel cells (MFCs) because of their high electrical conductivity. However, only a few types of metals have been used as anodes, and an extensive screening of metals has not yet been conducted. In this study, to develop a new metal anode for increased electricity generation in MFCs, 14 different metals (Al, Ti, Fe, Ni, Cu, Zn, Zr, Nb, Mo, Ag, In, Sn, Ta, and W) and 31 of their oxidized forms were comprehensively tested. Read More

    Alignment of microbial fitness with engineered product formation: obligatory coupling between acetate production and photoautotrophic growth.
    Biotechnol Biofuels 2018 13;11:38. Epub 2018 Feb 13.
    1Molecular Microbial Physiology Group, Faculty of Science, Swammerdam Institute for Life Sciences, University of Amsterdam, Science Park 904, 1098 XH Amsterdam, The Netherlands.
    Background: Microbial bioengineering has the potential to become a key contributor to the future development of human society by providing sustainable, novel, and cost-effective production pipelines. However, the sustained productivity of genetically engineered strains is often a challenge, as spontaneous non-producing mutants tend to grow faster and take over the population. Novel strategies to prevent this issue of strain instability are urgently needed. Read More

    Deep Eutectic Solvents pretreatment of agro-industrial food waste.
    Biotechnol Biofuels 2018 12;11:37. Epub 2018 Feb 12.
    2Dipartimento di Ingegneria Chimica, dei Materiali e della Produzione Industriale - Università degli Studi di Napoli Federico II, P.le V. Tecchio 80, 80125 Naples, Italy.
    Background: Waste biomass from agro-food industries are a reliable and readily exploitable resource. From the circular economy point of view, direct residues from these industries exploited for production of fuel/chemicals is a winning issue, because it reduces the environmental/cost impact and improves the eco-sustainability of productions.

    Results: The present paper reports recent results of deep eutectic solvent (DES) pretreatment on a selected group of the agro-industrial food wastes (AFWs) produced in Europe. Read More

    Improved photobio-Hproduction regulated by artificial miRNA targetingin green microalga.
    Biotechnol Biofuels 2018 12;11:36. Epub 2018 Feb 12.
    1Guangdong Technology Research Center for Marine Algal Bioengineering, Guangdong Key Laboratory of Plant Epigenetics, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen, 518060 People's Republic of China.
    Background: Sulfur-deprived cultivation of, referred as "two-stage culture" transferring the cells from regular algal medium to sulfur-deplete one, has been extensively studied to improve photobio-Hproduction in this green microalga. During sulfur-deprivation treatment, the synthesis of a key component of photosystem II complex, D1 protein, was inhibited and improved photobio-Hproduction could be established in. However, separation of algal cells from a regular liquid culture medium to a sulfur-deprived one is not only a discontinuous process, but also a cost- and time-consuming operation. Read More

    Production of (2R, 3R)-2,3-butanediol using engineered: strain construction, characterization and fermentation.
    Biotechnol Biofuels 2018 12;11:35. Epub 2018 Feb 12.
    Department of Chemical and Biological Engineering, University of Ottawa, 161 Louis Pasteur Private, Ottawa, ON K1N 6N5 Canada.
    Background: 2,3-butanediol (2,3-BD) is a bulk platform chemical with various potential applications such as aviation fuel. 2,3-BD has three optical isomers: (2R, 3R)-, (2S, 3S)- and meso-2,3-BD. Optically pure 2,3-BD is a crucial precursor for the chiral synthesis and it can also be used as anti-freeze agent due to its low freezing point. Read More

    Mathematical models of lignin biosynthesis.
    Biotechnol Biofuels 2018 9;11:34. Epub 2018 Feb 9.
    1The Wallace H. Coulter, Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, 313, Ferst Drive, Atlanta, GA 30332 USA.
    Background: Lignin is a natural polymer that is interwoven with cellulose and hemicellulose within plant cell walls. Due to this molecular arrangement, lignin is a major contributor to the recalcitrance of plant materials with respect to the extraction of sugars and their fermentation into ethanol, butanol, and other potential bioenergy crops. The lignin biosynthetic pathway is similar, but not identical in different plant species. Read More

    A copper-controlled RNA interference system for reversible silencing of target genes in.
    Biotechnol Biofuels 2018 9;11:33. Epub 2018 Feb 9.
    State Key Laboratory of Microbial Technology, School of Life Science, Shandong University, No.27 Shanda South Road, Jinan, 250100 Shandong People's Republic of China.
    Background: is a primary lignocellulosic enzyme producer in industry. However, the mechanisms underlying cellulase synthesis as well as other physiological processes are insufficiently understood partly due to the sophisticated process for its genetic manipulation. Target gene knockdown by RNA interference (RNAi) is a powerful tool for genetic research and biotechnology in eukaryotes including filamentous fungi. Read More

    Characterization and engineering of a dual-function diacylglycerol acyltransferase in the oleaginous marine diatom.
    Biotechnol Biofuels 2018 9;11:32. Epub 2018 Feb 9.
    2State Key Laboratory of Marine Resource Utilization in South China Sea, College of Oceanology, Hainan University, Haikou, Hainan 570228 China.
    Background: Photosynthetic oleaginous microalgae are promising feedstocks for biofuels. Acyl-CoA:diacylglycerol acyltransferases (DGATs) represent rich sources for engineering microalgal lipid production. The principal activity of DGATs has been defined as a single-function enzyme catalyzing the esterification of diacylglycerol with acyl-CoA. Read More

    A bionic system with Fenton reaction and bacteria as a model for bioprocessing lignocellulosic biomass.
    Biotechnol Biofuels 2018 8;11:31. Epub 2018 Feb 8.
    1School of Metallurgy and Environment, Central South University, Changsha, 410083 People's Republic of China.
    Background: The recalcitrance of lignocellulosic biomass offers a series of challenges for biochemical processing into biofuels and bio-products. For the first time, we address these challenges with a biomimetic system via a mild yet rapid Fenton reaction and lignocellulose-degrading bacterial strainB-8 (here after B-8) to pretreat the rice straw (RS) by mimicking the natural fungal invasion process. Here, we also elaborated the mechanism through conducting a systematic study of physicochemical changes before and after pretreatment. Read More

    Highly efficient conversion of plant oil to bio-aviation fuel and valuable chemicals by combination of enzymatic transesterification, olefin cross-metathesis, and hydrotreating.
    Biotechnol Biofuels 2018 7;11:30. Epub 2018 Feb 7.
    1National Energy Research Center for Biorefinery, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, 100029 People's Republic of China.
    Background: The production of fuels and chemicals from renewable resources is increasingly important due to the environmental concern and depletion of fossil fuel. Despite the fast technical development in the production of aviation fuels, there are still several shortcomings such as a high cost of raw materials, a low yield of aviation fuels, and poor process techno-economic consideration. In recent years, olefin metathesis has become a powerful and versatile tool for generating new carbon-carbon bonds. Read More

    Time-series lipidomic analysis of the oleaginous green microalga speciesunder nutrient stress.
    Biotechnol Biofuels 2018 6;11:29. Epub 2018 Feb 6.
    1Department of Chemistry, University at Buffalo, The State University of New York (SUNY), Buffalo, NY 14260 USA.
    Background: Microalgae are uniquely advantageous organisms cultured and harvested for several value-added biochemicals. A majority of these compounds are lipid-based, such as triacylglycerols (TAGs), which can be used for biofuel production, and their accumulation is most affected under nutrient stress conditions. As such, the balance between cellular homeostasis and lipid metabolism becomes more intricate to achieve efficiency in bioproduct synthesis. Read More

    Association of improved oxidative stress tolerance and alleviation of glucose repression with superior xylose-utilization capability by a natural isolate of.
    Biotechnol Biofuels 2018 5;11:28. Epub 2018 Feb 5.
    1State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240 China.
    Background: wild strains generally have poor xylose-utilization capability, which is a major barrier for efficient bioconversion of lignocellulosic biomass. Laboratory adaption is commonly used to enhance xylose utilization of recombinant. Apparently, yeast cells could remodel the metabolic network for xylose metabolism. Read More

    SACCHARIS: an automated pipeline to streamline discovery of carbohydrate active enzyme activities within polyspecific families and de novo sequence datasets.
    Biotechnol Biofuels 2018 5;11:27. Epub 2018 Feb 5.
    Lethbridge Research and Development Centre, Agriculture and Agri-Food Canada, 5403-1st Avenue South, Lethbridge, AB T1J 4B1 Canada.
    Background: Deposition of new genetic sequences in online databases is expanding at an unprecedented rate. As a result, sequence identification continues to outpace functional characterization of carbohydrate active enzymes (CAZymes). In this paradigm, the discovery of enzymes with novel functions is often hindered by high volumes of uncharacterized sequences particularly when the enzyme sequence belongs to a family that exhibits diverse functional specificities (i. Read More

    Re-direction of carbon flux to key precursor malonyl-CoA via artificial small RNAs in photosyntheticsp. PCC 6803.
    Biotechnol Biofuels 2018 5;11:26. Epub 2018 Feb 5.
    1Laboratory of Synthetic Microbiology, School of Chemical Engineering & Technology, Tianjin University, Tianjin, 300072 People's Republic of China.
    Background: Photosynthetic cyanobacteria have attracted a significant attention as promising chassis to produce renewable fuels and chemicals due to their capability to utilizing solar energy and CO. Notably, the enhancing supply of key precursors like malonyl-CoA would benefit the production of many bio-compounds. Nevertheless, the lacking of genetic tools in cyanobacteria, especially the knockdown strategies for essential pathways, has seriously restricted the attempts to re-direct carbon flux from the central carbohydrate metabolism to the synthesis of bioproducts. Read More

    Quantitative trait loci for cell wall composition traits measured using near-infrared spectroscopy in the model C4 perennial grass.
    Biotechnol Biofuels 2018 3;11:25. Epub 2018 Feb 3.
    1Department of Integrative Biology, The University of Texas at Austin, Austin, TX 78712 USA.
    Background: Biofuels derived from lignocellulosic plant material are an important component of current renewable energy strategies. Improvement efforts in biofuel feedstock crops have been primarily focused on increasing biomass yield with less consideration for tissue quality or composition. Four primary components found in the plant cell wall contribute to the overall quality of plant tissue and conversion characteristics, cellulose and hemicellulose polysaccharides are the primary targets for fuel conversion, while lignin and ash provide structure and defense. Read More

    Transcriptome analysis of the digestive system of a wood-feeding termite () revealed a unique mechanism for effective biomass degradation.
    Biotechnol Biofuels 2018 3;11:24. Epub 2018 Feb 3.
    1Biofuels Institute of Jiangsu University, School of Environmental and Safety Engineering, Jiangsu University, Zhenjiang, 212013 Jiangsu China.
    Background: Wood-feeding termite,Shiraki, represents a highly efficient system for biomass deconstruction and utilization. However, the detailed mechanisms of lignin modification and carbohydrate degradation in this system are still largely elusive.

    Results: In order to reveal the inherent mechanisms for efficient biomass degradation, four different organs (salivary glands, foregut, midgut, and hindgut) within a complete digestive system of a lower termite,, were dissected and collected. Read More

    Life-cycle assessment of transportation biofuels from hydrothermal liquefaction of forest residues in British Columbia.
    Biotechnol Biofuels 2018 3;11:23. Epub 2018 Feb 3.
    1Department of Chemical and Biological Engineering, University of British Columbia, 2360 East Mall, Vancouver, BC V6T 1Z3 Canada.
    Background: Biofuels from hydrothermal liquefaction (HTL) of abundantly available forest residues in British Columbia (BC) can potentially make great contributions to reduce the greenhouse gas (GHG) emissions from the transportation sector. A life-cycle assessment was conducted to quantify the GHG emissions of a hypothetic 100 million liters per year HTL biofuel system in the Coast Region of BC. Three scenarios were defined and investigated, namely, supply of bulky forest residues for conversion in a central integrated refinery (Fr-CIR), HTL of forest residues to bio-oil in distributed biorefineries and subsequent upgrading in a central oil refinery (Bo-DBR), and densification of forest residues in distributed pellet plants and conversion in a central integrated refinery (Wp-CIR). Read More

    High activity CAZyme cassette for improving biomass degradation in thermophiles.
    Biotechnol Biofuels 2018 1;11:22. Epub 2018 Feb 1.
    1Biosciences Center, National Renewable Energy Laboratory, 15013 Denver West Parkway, Golden, CO 80401 USA.
    Background: Thermophilic microorganisms and their enzymes offer several advantages for industrial application over their mesophilic counterparts. For example, a hyperthermophilic anaerobe,, was recently isolated from hot springs in Kamchatka, Siberia, and shown to have very high cellulolytic activity. Additionally, it is one of a few microorganisms being considered as viable candidates for consolidated bioprocessing applications. Read More

    Combinatorial pretreatment and fermentation optimization enabled a record yield on lignin bioconversion.
    Biotechnol Biofuels 2018 29;11:21. Epub 2018 Jan 29.
    1Synthetic and Systems Biology Innovation Hub (SSBiH), Texas A&M University, College Station, TX 77843 USA.
    Background: Lignin valorization has recently been considered to be an essential process for sustainable and cost-effective biorefineries. Lignin represents a potential new feedstock for value-added products. Oleaginous bacteria such ascan produce intracellular lipids from biodegradation of aromatic substrates. Read More

    Processivity and enzymatic mechanism of a multifunctional family 5 endoglucanase fromBS-5 with potential applications in the saccharification of cellulosic substrates.
    Biotechnol Biofuels 2018 29;11:20. Epub 2018 Jan 29.
    3School of Chemistry and Molecular Biosciences, The University of Queensland, St. Lucia, Brisbane, QLD 4072 Australia.
    Background: Presently, enzymes still constitute a major part of the cost of biofuel production from lignocellulosic biomass. Processive endoglucanases, which possess both endoglucanase and exoglucanase activity, have the potential to reduce the costs of biomass saccharification when used together with commercial cellulases. Therefore, the exploration of new processive endoglucanases has attracted much attention with a view to accelerating the industrialization of biofuels and biochemicals. Read More

    Enhancing methane production from lignocellulosic biomass by combined steam-explosion pretreatment and bioaugmentation with cellulolytic bacterium.
    Biotechnol Biofuels 2018 29;11:19. Epub 2018 Jan 29.
    1Faculty of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences, P.O.Box 5003, 1432 Ås, Norway.
    Background: Biogas production from lignocellulosic biomass is generally considered to be challenging due to the recalcitrant nature of this biomass. In this study, the recalcitrance of birch was reduced by applying steam-explosion (SE) pretreatment (210 °C and 10 min). Moreover, bioaugmentation with the cellulolytic bacteriumwas applied to possibly enhance the methane production from steam-exploded birch in an anaerobic digestion (AD) process under thermophilic conditions (62 °C). Read More

    Purification of 2,3-butanediol from fermentation broth: process development and techno-economic analysis.
    Biotechnol Biofuels 2018 25;11:18. Epub 2018 Jan 25.
    School of Chemical Engineering, Yeungnam University, Dae-dong 214-1, Gyeongsan, 38541 Republic of Korea.
    Background: 2,3-Butanediol (2,3-BDO) is a synthetic chemical compound that also can be produced by biomass fermentation, which is gaining share in the global market as an intermediate product for numerous applications, i.e. as liquid fuel or fuel additive. Read More

    Optimizing anaerobic growth rate and fermentation kinetics instrains expressing Calvin-cycle enzymes for improved ethanol yield.
    Biotechnol Biofuels 2018 25;11:17. Epub 2018 Jan 25.
    1Department of Biotechnology, Delft University of Technology, Van der Maasweg 9, 2629 HZ Delft, The Netherlands.
    Background: Reduction or elimination of by-product formation is of immediate economic relevance in fermentation processes for industrial bioethanol production with the yeast. Anaerobic cultures of wild-typerequire formation of glycerol to maintain the intracellular NADH/NADbalance. Previously, functional expression of the Calvin-cycle enzymes ribulose-1,5-bisphosphate carboxylase (RuBisCO) and phosphoribulokinase (PRK) inwas shown to enable reoxidation of NADH with COas electron acceptor. Read More

    Physico-chemical oxidative cleavage strategy facilitates the degradation of recalcitrant crystalline cellulose by cellulases hydrolysis.
    Biotechnol Biofuels 2018 25;11:16. Epub 2018 Jan 25.
    Beijing Key Laboratory of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, 100029 China.
    Background: Efficient enzymatic conversion of recalcitrant crystalline cellulose is critical for enabling cost-effective industrial conversion of cellulosic biomass to biofuels and chemicals. Fully understanding enzyme digestion mechanism is paving a new way to design efficient process for biomass conversion. Accordingly, a continuing drive is inspiring to discover new routes to promote crystalline cellulose disruption. Read More

    Disruption of zinc finger DNA binding domain in catabolite repressor Mig1 increases growth rate, hyphal branching, and cellulase expression in hypercellulolytic fungusNCIM1228.
    Biotechnol Biofuels 2018 25;11:15. Epub 2018 Jan 25.
    1Microbial Engineering Group, International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi, 110067 India.
    Background: There is an urgent requirement for second-generation bio-based industries for economical yet efficient enzymatic cocktail to convert diverse cellulosic biomass into fermentable sugars. In our previous study, secretome ofNCIM1228 showed high commercial potential by exhibiting high biomass hydrolyzing efficiency. To develop NCIM1228 further as an industrial workhorse, one of the major genetic interventions needed is global deregulation of cellulolytic genes to achieve higher enzyme production. Read More

    A systematic optimization of styrene biosynthesis inBL21(DE3).
    Biotechnol Biofuels 2018 25;11:14. Epub 2018 Jan 25.
    1CAS Key Laboratory of Biobased Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, No.189 Songling Road, Laoshan District, Qingdao, 266101 China.
    Background: Styrene is a versatile commodity petrochemical used as a monomer building-block for the synthesis of many useful polymers. Although achievements have been made on styrene biosynthesis in microorganisms, several bottleneck problems limit factors for further improvement in styrene production.

    Results: A two-step styrene biosynthesis pathway was developed and introduced intoBL21(DE3). Read More

    Organic acid production from potato starch waste fermentation by rumen microbial communities from Dutch and Thai dairy cows.
    Biotechnol Biofuels 2018 25;11:13. Epub 2018 Jan 25.
    1Laboratory of Microbiology, Wageningen University & Research, Stippeneng 4, 6708 WE Wageningen, The Netherlands.
    Background: Exploring different microbial sources for biotechnological production of organic acids is important. Dutch and Thai cow rumen samples were used as inocula to produce organic acid from starch waste in anaerobic reactors. Organic acid production profiles were determined and microbial communities were compared using 16S ribosomal ribonucleic acid gene amplicon pyrosequencing. Read More

    Enhanced isopropanol-butanol-ethanol mixture production through manipulation of intracellular NAD(P)H level in the recombinantXY16.
    Biotechnol Biofuels 2018 24;11:12. Epub 2018 Jan 24.
    1State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Puzhu South Road 30#, Nanjing, 211816 People's Republic of China.
    Background: The formation of by-products, mainly acetone in acetone-butanol-ethanol (ABE) fermentation, significantly affects the solvent yield and downstream separation process. In this study, we genetically engineeredXY16 isolated by our lab to eliminate acetone production and altered ABE to isopropanol-butanol-ethanol (IBE). Meanwhile, process optimization under pH control strategies and supplementation of calcium carbonate were adopted to investigate the interaction between the reducing force of the metabolic networks and IBE production. Read More

    Engineeringto enhance lipid production from lignocellulosic materials.
    Biotechnol Biofuels 2018 22;11:11. Epub 2018 Jan 22.
    1Micalis Institute, INRA, AgroParisTech, Université Paris-Saclay, 78350 Jouy-en-Josas, France.
    Background: is a common biotechnological chassis for the production of lipids, which are the preferred feedstock for the production of fuels and chemicals. To reduce the cost of microbial lipid production, inexpensive carbon sources must be used, such as lignocellulosic hydrolysates. Unfortunately, lignocellulosic materials often contain toxic compounds and a large amount of xylose, which cannot be used by. Read More

    Laccase-derived lignin compounds boost cellulose oxidative enzymes AA9.
    Biotechnol Biofuels 2018 17;11:10. Epub 2018 Jan 17.
    1Faculty of Science, Department of Geosciences and Natural Resource Management, University of Copenhagen, Frederiksberg C, Denmark.
    Background: The discovery of lignin as activator for the redox enzyme lytic polysaccharide monooxygenases (LPMOs) for the oxidation of cell-wall polysaccharides opens a new scenario for investigation of the interplay between different lignocellulose-degrading enzymes. The lignin-active enzymes in one hand, and the carbohydrate active in the other, are linked through a variety of electrons carrier molecules either derived from lignin or enzymatically transferred. Likewise, in nature, many lignocellulose-degrading organisms are expressing those enzymes simultaneously, and we wanted to test if a major commercial available lignin oxidase enzyme, i. Read More

    Working towards recalcitrance mechanisms: increased xylan and homogalacturonan production by overexpression of() causes increased recalcitrance and decreased growth in.
    Biotechnol Biofuels 2018 17;11. Epub 2018 Jan 17.
    1Department of Biochemistry and Molecular Biology, University of Georgia, Athens, GA 30602 USA.
    Background: The development of fast-growing hardwood trees as a source of lignocellulosic biomass for biofuel and biomaterial production requires a thorough understanding of the plant cell wall structure and function that underlie the inherent recalcitrance properties of woody biomass. Downregulation ofinwas recently reported to result in improved biomass saccharification, plant growth, and biomass yield. To further understandfunction in biomass recalcitrance and plant growth, here we report the effects ofoverexpression in. Read More

    In vivo selection of sfGFP variants with improved and reliable functionality in industrially important thermophilic bacteria.
    Biotechnol Biofuels 2018 17;11. Epub 2018 Jan 17.
    1Department of Molecular Genetics, Groningen Biomolecular Sciences and Biotechnology Institute, Centre for Synthetic Biology, University of Groningen, Nijenborgh 7, 9747 AG Groningen, The Netherlands.
    Background: Fluorescent reporter proteins (FP) have become an indispensable tool for the optimization of microbial cell factories and in synthetic biology per se. The applicability of the currently available FPs is, however, constrained by species-dependent performance and misfolding at elevated temperatures. To obtain functional reporters for thermophilic, biotechnologically important bacteria such as, an in vivo screening approach based on a mutational library of superfolder GFP was applied. Read More

    Conversion of lignocellulosic agave residues into liquid biofuels using an AFEX™-based biorefinery.
    Biotechnol Biofuels 2018 16;11. Epub 2018 Jan 16.
    2Department of Chemical Engineering and Materials Science, Michigan State University, 3815 Technology Boulevard, Lansing, MI 48910 USA.
    Background: Agave-based alcoholic beverage companies generate thousands of tons of solid residues per year in Mexico. These agave residues might be used for biofuel production due to their abundance and favorable sustainability characteristics. In this work, agave leaf and bagasse residues from speciesandwere subjected to pretreatment using the ammonia fiber expansion (AFEX) process. Read More

    Determination of the native features of the exoglucanase Cel48S from.
    Biotechnol Biofuels 2018 13;11. Epub 2018 Jan 13.
    1Shandong Provincial Key Laboratory of Energy Genetics, CAS Key Laboratory of Biofuels, Qingdao Engineering Laboratory of Single Cell Oil, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, 266101 People's Republic of China.
    Background: is considered one of the most efficient natural cellulose degraders because of its cellulosomal system. As the major exoglucanase of cellulosome in, Cel48S plays key roles and influences the activity and features of cellulosome to a great extent. Thus, it is of great importance to reveal the enzymatic features of Cel48S. Read More

    Correlation of structure, function and protein dynamics in GH7 cellobiohydrolases from,and.
    Biotechnol Biofuels 2018 13;11. Epub 2018 Jan 13.
    1Department of Molecular Sciences, Swedish University of Agricultural Sciences, P.O. Box 7015, 750 07 Uppsala, Sweden.
    Background: The ascomycete fungusis the predominant source of enzymes for industrial conversion of lignocellulose. Its glycoside hydrolase family 7 cellobiohydrolase (GH7 CBH)Cel7A constitutes nearly half of the enzyme cocktail by weight and is the major workhorse in the cellulose hydrolysis process. The orthologs from(Cel7A) and(Cel7A) show high sequence identity withCel7A, ~ 80%, and represent naturally evolved combinations of cellulose-binding tunnel-enclosing loop motifs, which have been suggested to influence intrinsic cellobiohydrolase properties, such as endo-initiation, processivity, and off-rate. Read More

    One-step production of C6-C8 carboxylates by mixed culture solely grown on CO.
    Biotechnol Biofuels 2018 9;11. Epub 2018 Jan 9.
    State Key Laboratory of Pollution Control and Resource Reuse, Tongji University, Shanghai, 200092 People's Republic of China.
    Background: This study aimed at producing C6-C8 medium-chain carboxylates (MCCAs) directly from gaseous CO using mixed culture. The yield and C2-C8 product composition were investigated when CO was continuously fed with gradually increasing partial pressure.

    Results: The maximal concentrations of-caproate,-heptylate, and-caprylate were 1. Read More

    Construction of an ultrahigh-density genetic linkage map forL. and identification of QTL for fruit yield.
    Biotechnol Biofuels 2018 9;11. Epub 2018 Jan 9.
    The Institute of Tropical Biosciences and Biotechnology, Chinese Academy of Tropical Agriculture Sciences, Haikou, China.
    Background: As an important biofuel plant, the demand for higher yieldL. is rapidly increasing. However, genetic analysis ofand molecular breeding for higher yield have been hampered by the limited number of molecular markers available. Read More

    Gene stacking of multiple traits for high yield of fermentable sugars in plant biomass.
    Biotechnol Biofuels 2018 9;11. Epub 2018 Jan 9.
    Feedstocks Division, Joint BioEnergy Institute, Lawrence Berkeley National Laboratory, Berkeley, CA 94720 USA.
    Background: Second-generation biofuels produced from biomass can help to decrease dependency on fossil fuels, bringing about many economic and environmental benefits. To make biomass more suitable for biorefinery use, we need a better understanding of plant cell wall biosynthesis. Increasing the ratio of C6 to C5 sugars in the cell wall and decreasing the lignin content are two important targets in engineering of plants that are more suitable for downstream processing for second-generation biofuel production. Read More

    Metabolic engineering offor production of isobutanol and isobutyl acetate.
    Biotechnol Biofuels 2018 8;11. Epub 2018 Jan 8.
    National Center for Genetic Engineering and Biotechnology, 113 Thailand Science Park, Paholyothin Road, Klong 1, Klong Luang, Pathumthani 12120 Thailand.
    Background: Interests in renewable fuels have exploded in recent years as the serious effects of global climate change become apparent. Microbial production of high-energy fuels by economically efficient bioprocesses has emerged as an attractive alternative to the traditional production of transportation fuels. Here, we engineered, an industrial workhorse in heterologous enzyme production, to produce the biofuel isobutanol from two renewable carbon sources, glucose and glycerol. Read More

    Overexpression of a Domain of Unknown Function 231-containing protein increases O-xylan acetylation and cellulose biosynthesis in.
    Biotechnol Biofuels 2017 27;10:311. Epub 2017 Dec 27.
    BioEnergy Science Center and Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831 USA.
    Background: Domain of Unknown Function 231-containing proteins (DUF231) are plant specific and their function is largely unknown. Studies in the model plants Arabidopsis and rice suggested that some DUF231 proteins act in the process of-acetyl substitution of hemicellulose and esterification of pectin. However, little is known about the function of DUF231 proteins in woody plant species. Read More

    Enhanced rates of enzymatic saccharification and catalytic synthesis of biofuel substrates in gelatinized cellulose generated by trifluoroacetic acid.
    Biotechnol Biofuels 2017 27;10:310. Epub 2017 Dec 27.
    Laboratory of Renewable Resources Engineering, Purdue University, West Lafayette, IN 47907 USA.
    Background: The crystallinity of cellulose is a principal factor limiting the efficient hydrolysis of biomass to fermentable sugars or direct catalytic conversion to biofuel components. We evaluated the impact of TFA-induced gelatinization of crystalline cellulose on enhancement of enzymatic digestion and catalytic conversion to biofuel substrates.

    Results: Low-temperature swelling of cotton linter cellulose in TFA at subzero temperatures followed by gentle heating to 55 °C dissolves the microfibril structure and forms composites of crystalline and amorphous gels upon addition of ethanol. Read More

    Development and use of a switchgrass (L.) transformation pipeline by the BioEnergy Science Center to evaluate plants for reduced cell wall recalcitrance.
    Biotechnol Biofuels 2017 22;10:309. Epub 2017 Dec 22.
    BioEnergy Science Center (BESC), Oak Ridge National Laboratory, Oak Ridge, TN 37831 USA.
    Background: The mission of the BioEnergy Science Center (BESC) was to enable efficient lignocellulosic-based biofuel production. One BESC goal was to decrease poplar and switchgrass biomass recalcitrance to biofuel conversion while not affecting plant growth. A transformation pipeline (TP), to express transgenes or transgene fragments (constructs) in these feedstocks with the goal of understanding and decreasing recalcitrance, was considered essential for this goal. Read More

    Isolation, phenotypic characterization and genome wide analysis of astrain naturally modified under laboratory conditions: towards enhanced microalgal biomass and lipid production for biofuels.
    Biotechnol Biofuels 2017 22;10:308. Epub 2017 Dec 22.
    Department of Chemical and Biomolecular Engineering, KAIST, 291 Daehak-ro, Yuseong-gu, Daejeon, 34141 Republic of Korea.
    Background: Microalgal strain development through genetic engineering has received much attention as a way to improve the traits of microalgae suitable for biofuel production. However, there are still some limitations in application of genetically modified organisms. In this regard, there has been recent interest in the isolation and characterization of superior strains naturally modified and/or adapted under a certain condition and on the interpretation of phenotypic changes through the whole genome sequencing. Read More

    Identification of alcohol stress tolerance genes ofsp. PCC 6803 using adaptive laboratory evolution.
    Biotechnol Biofuels 2017 20;10:307. Epub 2017 Dec 20.
    Department of Bioinformatic Engineering, Graduate School of Information Science and Technology, Osaka University, 1-5 Yamadaoka, Suita, Osaka 565-0871 Japan.
    Background: sp. PCC 6803 is an attractive organism for the production of alcohols, such as isobutanol and ethanol. However, because stress against the produced alcohol is a major barrier for industrial applications, it is highly desirable to engineer organisms with strong alcohol tolerance. Read More

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