1,756 results match your criteria Biotechnology for Biofuels [Journal]


Improved production of succinic acid from growing on and process evaluation through material flow analysis.

Biotechnol Biofuels 2019 4;12:22. Epub 2019 Feb 4.

Department of Experimental Medicine, University of Campania L. Vanvitelli, Via de Crecchio 7, 80138 Naples, Italy.

Background: Due to its wide range of applications in the food, pharmaceutical and chemical fields, microbial synthesis of succinic acid is receiving growing attention, generating already relevant industrial results, as well as fueling constant research for improvements. In order to develop a sustainable process, a special focus is now set on the exploitation and conversion of lignocellulosic biomasses into platform chemicals.

Results: In the present work we used BPP7 in separated hydrolysis and fermentation experiments with as starting material. Read More

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http://dx.doi.org/10.1186/s13068-019-1362-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6360672PMC
February 2019

Proteomic study uncovers molecular principles of single-cell-level phenotypic heterogeneity in lipid storage of .

Biotechnol Biofuels 2019 4;12:21. Epub 2019 Feb 4.

2Plant Biochemistry, Ruhr University Bochum, 44801 Bochum, Germany.

Background: belongs to a large group of photoautotrophic eukaryotic organisms that play important roles in fixation and cycling of atmospheric CO. Its capability of storing solar energy and carbon dioxide in the form of triacylglycerol (TAG) of up to 60% of total weight under nitrogen deprivation stress sparked interest in its use for biofuel production. Phenotypes varying in lipid accumulation among an population can be disclosed by single-cell analysis/sorting using fluorescence-activated cell sorting (FACS); yet the phenomenon of single cell heterogeneity in an algae population remains to be fully understood at the molecular level. Read More

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http://dx.doi.org/10.1186/s13068-019-1361-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6360718PMC
February 2019

Xylose fermentation efficiency of industrial yeast with separate or combined xylose reductase/xylitol dehydrogenase and xylose isomerase pathways.

Biotechnol Biofuels 2019 28;12:20. Epub 2019 Jan 28.

1CEB-Centre of Biological Engineering, University of Minho, 4710-057 Braga, Portugal.

Background: Xylose isomerase (XI) and xylose reductase/xylitol dehydrogenase (XR/XDH) pathways have been extensively used to confer xylose assimilation capacity to and tackle one of the major bottlenecks in the attainment of economically viable lignocellulosic ethanol production. Nevertheless, there is a lack of studies comparing the efficiency of those pathways both separately and combined. In this work, the XI and/or XR/XDH pathways were introduced into two robust industrial strains, evaluated in synthetic media and corn cob hemicellulosic hydrolysate and the results were correlated with the differential enzyme activities found in the xylose-pathway engineered strains. Read More

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http://dx.doi.org/10.1186/s13068-019-1360-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6348659PMC
January 2019

Existence of a scaling relation in continuous cultures of : the steady states are completely determined by the ratio of carbon and oxygen uptake rates.

Biotechnol Biofuels 2019 28;12:19. Epub 2019 Jan 28.

Department of Biochemical Engineering and Biotechnology, Indian Institute of Technology Delhi, Hauz Khas, New Delhi, 110016 India.

Background: Recently, we showed that steady-state continuous cultures of   follow the principles of growth on mixture of two complementary substrates. More precisely, when such cultures are fed with progressively higher concentrations of glucose at fixed dilution rate  = 0.1 h, oxygen mass-transfer coefficient  = 50 h, and oxygen solubility , they transition from glucose- to oxygen-limited growth through an intermediate dual-limited regime in which both glucose and oxygen are limiting, and ethanol is produced without loss of glucose. Read More

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http://dx.doi.org/10.1186/s13068-019-1357-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6348663PMC
January 2019

Comparative transcriptome analysis of a long-time span two-step culture process reveals a potential mechanism for astaxanthin and biomass hyper-accumulation in JNU35.

Biotechnol Biofuels 2019 28;12:18. Epub 2019 Jan 28.

Department of Ecology, Institute of Hydrobiology, College of Life Science and Technology, Jinan University, No.601 Huangpu Road, Tianhe District, Guangzhou, 510632 Guangdong People's Republic of China.

Background: Among all organisms tested, can accumulate the highest levels of natural astaxanthin. Nitrogen starvation and high irradiance promote the accumulation of starch, lipid, and astaxanthin in . , yet their cell division is significantly retarded. Read More

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http://dx.doi.org/10.1186/s13068-019-1355-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6348685PMC
January 2019

Methanol fermentation increases the production of NAD(P)H-dependent chemicals in synthetic methylotrophic .

Biotechnol Biofuels 2019 21;12:17. Epub 2019 Jan 21.

State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing, 211816 Jiangsu China.

Background: Methanol has attracted increased attention as a non-food alternative carbon source to sugar for biological production of chemicals and fuels. Moreover, the high degree of reduction of methanol offers some advantages in increasing the production yields of NAD(P)H-dependent metabolites. Here, we demonstrate an example of methanol bioconversion with the aim of improving production of NAD(P)H-dependent chemicals in synthetic methylotrophic . Read More

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http://dx.doi.org/10.1186/s13068-019-1356-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6340170PMC
January 2019

Identification of genes associated with ricinoleic acid accumulation in via transcriptome analysis.

Biotechnol Biofuels 2019 21;12:16. Epub 2019 Jan 21.

2Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, AB T6G 2P5 Canada.

Background: Ricinoleic acid is a high-value hydroxy fatty acid with broad industrial applications. seed oil contains a high amount of ricinoleic acid (~ 80%) and represents an emerging source of this unusual fatty acid. However, the mechanism of ricinoleic acid accumulation in is yet to be explored at the molecular level, which hampers the exploration of its potential in ricinoleic acid production. Read More

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http://dx.doi.org/10.1186/s13068-019-1358-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6340187PMC
January 2019
1 Read

Multiple levers for overcoming the recalcitrance of lignocellulosic biomass.

Biotechnol Biofuels 2019 17;12:15. Epub 2019 Jan 17.

1Thayer School of Engineering, Dartmouth College, 14 Engineering drive, Hanover, NH 03755 USA.

Background: The recalcitrance of cellulosic biomass is widely recognized as a key barrier to cost-effective biological processing to fuels and chemicals, but the relative impacts of physical, chemical and genetic interventions to improve biomass processing singly and in combination have yet to be evaluated systematically. Solubilization of plant cell walls can be enhanced by non-biological augmentation including physical cotreatment and thermochemical pretreatment, the choice of biocatalyst, the choice of plant feedstock, genetic engineering of plants, and choosing feedstocks that are less recalcitrant natural variants. A two-tiered combinatoric investigation of lignocellulosic biomass deconstruction was undertaken with three biocatalysts (, Novozymes Cellic Ctec2 and Htec2), three transgenic switchgrass plant lines (COMT, MYB4, GAUT4) and their respective nontransgenic controls, two natural variants, and augmentation of biological attack using either mechanical cotreatment or cosolvent-enhanced lignocellulosic fractionation (CELF) pretreatment. Read More

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http://dx.doi.org/10.1186/s13068-019-1353-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6335785PMC
January 2019

Characterization of involved in fatty acid transport for oil accumulation in the green alga .

Biotechnol Biofuels 2019 12;12:14. Epub 2019 Jan 12.

1Research Center of Bioremediation and Bioenergy, College of Resources and Environment, Southwest University, Beibei, Chongqing, 400715 People's Republic of China.

Background: In the past few decades, microalgae biofuel has become one of the most interesting sources of renewable energy. However, the higher cost of microalgae biofuel compared to that of petroleum prevented microalgae biofuel production. Therefore, the research on increasing lipid productivity from microalgae becomes more important. Read More

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https://biotechnologyforbiofuels.biomedcentral.com/articles/
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http://dx.doi.org/10.1186/s13068-018-1332-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6330502PMC
January 2019
6 Reads

Elucidation of degrading pattern and substrate recognition of a novel bifunctional alginate lyase from sp. NJ-04 and its use for preparation alginate oligosaccharides.

Biotechnol Biofuels 2019 10;12:13. Epub 2019 Jan 10.

1College of Food Science and Light Industry, Nanjing Tech University, 30 Puzhu Rd, Nanjing, 211816 People's Republic of China.

Background: The alginate oligosaccharides have been widely used in agriculture, medicine, and food industries due to their versatile physiological functions such as antioxidant, anticoagulant, and antineoplastic activities. The bifunctional alginate lyases can degrade the alginate polysaccharide more efficiently into alginate oligosaccharides. Therefore, it is crucial to discover new bifunctional alginate lyase for alginate oligosaccharide production. Read More

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http://dx.doi.org/10.1186/s13068-019-1352-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6327446PMC
January 2019
1 Read

Overcoming biomass recalcitrance by synergistic pretreatment of mechanical activation and metal salt for enhancing enzymatic conversion of lignocellulose.

Biotechnol Biofuels 2019 10;12:12. Epub 2019 Jan 10.

1School of Chemistry and Chemical Engineering, Guangxi University, Nanning, 530004 China.

Background: Due to biomass recalcitrance, including complexity of lignocellulosic matrix, crystallinity of cellulose, and inhibition of lignin, the bioconversion of lignocellulosic biomass is difficult and inefficient. The aim of this study is to investigate an effective and green pretreatment method for overcoming biomass recalcitrance of lignocellulose.

Results: An effective mechanical activation (MA) + metal salt (MAMS) technology was applied to pretreat sugarcane bagasse (SCB), a typical kind of lignocellulosic biomass, in a stirring ball mill. Read More

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http://dx.doi.org/10.1186/s13068-019-1354-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6327530PMC
January 2019
2 Reads

Overproduction of native endo-β-1,4-glucanases leads to largely enhanced biomass saccharification and bioethanol production by specific modification of cellulose features in transgenic rice.

Biotechnol Biofuels 2019 9;12:11. Epub 2019 Jan 9.

1Biomass and Bioenergy Research Centre, College of Plant Science and Technology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, 430070 China.

Background: Genetic modification of plant cell walls has been implemented to reduce lignocellulosic recalcitrance for biofuel production. Plant glycoside hydrolase family 9 (GH9) comprises endo-β-1,4-glucanase in plants. Few studies have examined the roles of GH9 in cell wall modification. Read More

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https://biotechnologyforbiofuels.biomedcentral.com/articles/
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http://dx.doi.org/10.1186/s13068-018-1351-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6325865PMC
January 2019
5 Reads

Engineered tolerant to acetic acid and low pH via multiplex atmospheric and room temperature plasma mutagenesis.

Biotechnol Biofuels 2019 5;12:10. Epub 2019 Jan 5.

1Biomass Energy Technology Research Centre, Key Laboratory of Development and Application of Rural Renewable Energy (Ministry of Agriculture), Biogas Institute of Ministry of Agriculture, Section 4-13, Renmin Rd. South, Chengdu, 610041 China.

Background: Cellulosic biofuels are sustainable compared to fossil fuels. However, inhibitors, such as acetic acid generated during lignocellulose pretreatment and hydrolysis, would significantly inhibit microbial fermentation efficiency. Microbial mutants able to tolerate high concentration of acetic acid are needed urgently to alleviate this inhibition. Read More

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https://biotechnologyforbiofuels.biomedcentral.com/articles/
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http://dx.doi.org/10.1186/s13068-018-1348-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6321654PMC
January 2019
4 Reads
6.044 Impact Factor

PLDα1-knockdown soybean seeds display higher unsaturated glycerolipid contents and seed vigor in high temperature and humidity environments.

Biotechnol Biofuels 2019 4;12. Epub 2019 Jan 4.

1State Key Laboratory of Tea Plant Biology and Utilization, College of Tea and Food Science and Technology, Anhui Agricultural University, Hefei, 230036 China.

Background: Soybean oil constitutes an important source of vegetable oil and biofuel. However, high temperature and humidity adversely impacts soybean seed development, yield, and quality during plant development and after harvest. Genetic improvement of soybean tolerance to stress environments is highly desirable. Read More

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http://dx.doi.org/10.1186/s13068-018-1340-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6319013PMC
January 2019
5 Reads

Improved lipid production via fatty acid biosynthesis and free fatty acid recycling in engineered sp. PCC 6803.

Biotechnol Biofuels 2019 4;12. Epub 2019 Jan 4.

1Laboratory of Cyanobacterial Biotechnology, Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok, 10330 Thailand.

Background: Cyanobacteria are potential sources for third generation biofuels. Their capacity for biofuel production has been widely improved using metabolically engineered strains. In this study, we employed metabolic engineering design with target genes involved in selected processes including the fatty acid synthesis (a cassette of , , and encoding acetyl-CoA carboxylase, ACC), phospholipid hydrolysis ( encoding lipase A), alkane synthesis ( encoding acyl-ACP reductase, AAR), and recycling of free fatty acid (FFA) ( encoding acyl-acyl carrier protein synthetase, AAS) in the unicellular cyanobacterium sp. Read More

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http://dx.doi.org/10.1186/s13068-018-1349-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6319012PMC
January 2019
1 Read

Identification of an essential regulator controlling the production of raw-starch-digesting glucoamylase in .

Biotechnol Biofuels 2019 4;12. Epub 2019 Jan 4.

State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangxi Research Center for Microbial and Enzyme Engineering Technology, College of Life Science and Technology, Guangxi University, 100 Daxue Road, Nanning, 530004 Guangxi People's Republic of China.

Background: Raw-starch-digesting glucoamylases (RSDGs) from filamentous fungi have great commercial values in starch processing; however, the regulatory mechanisms associated with their production in filamentous fungi remain unknown. HP7-1 isolated by our laboratory secretes RSDG with suitable properties but at low production levels. Here, we screened and identified novel regulators of RSDG gene expression in . Read More

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http://dx.doi.org/10.1186/s13068-018-1345-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6318894PMC
January 2019
1 Read

Comprehensive evaluation of fuel properties and complex regulation of intracellular transporters for high oil production in developing seeds of for woody biodiesel.

Biotechnol Biofuels 2019 4;12. Epub 2019 Jan 4.

1Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, College of Biological Sciences and Biotechnology, School of Soil and Water Conservation, National Engineering Laboratory for Tree Breeding, Beijing Forestry University, Beijing, 100083 China.

Background: Based on our previous studies of 17 germplasms, one plus tree with high quality and quantity of seed oils has emerged as novel potential source of biodiesel. To better develop seed oils as woody biodiesel, a concurrent exploration of oil content, FA composition, biodiesel yield and fuel properties as well as prediction model construction for fuel properties was conducted on developing seeds to determine the optimal seed harvest time for producing high-quality biodiesel. Oil synthesis required supply of carbon source, energy and FA, but their transport mechanisms still remains enigmatic. Read More

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https://biotechnologyforbiofuels.biomedcentral.com/articles/
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http://dx.doi.org/10.1186/s13068-018-1347-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6318995PMC
January 2019
4 Reads
6.044 Impact Factor

Exploring the roles of and interactions among microbes in dry co-digestion of food waste and pig manure using high-throughput 16S rRNA gene amplicon sequencing.

Biotechnol Biofuels 2019 4;12. Epub 2019 Jan 4.

6Department of Science, Waterford Institute of Technology, Waterford, Ireland.

Background: With the increasing global population and increasing demand for food, the generation of food waste and animal manure increases. Anaerobic digestion is one of the best available technologies for food waste and pig manure management by producing methane-rich biogas. Dry co-digestion of food waste and pig manure can significantly reduce the reactor volume, capital cost, heating energy consumption and the cost of digestate liquid management. Read More

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http://dx.doi.org/10.1186/s13068-018-1344-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6318937PMC
January 2019
1 Read

Effects of amino acids on the lignocellulose degradation by Z5: insights into performance, transcriptional, and proteomic profiles.

Biotechnol Biofuels 2019 4;12. Epub 2019 Jan 4.

Jiangsu Key Lab for Organic Solid Waste Utilization, Nanjing, 210095 China.

Background: As a ubiquitous filamentous fungal, spp. play a critical role in lignocellulose degradation, which was also defined as considerable cell factories for organic acids and industrially relevant enzymes producer. Nevertheless, the production of various extracellular enzymes can be influenced by different factors including nitrogen source, carbon source, cultivation temperature, and initial pH value. Read More

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http://dx.doi.org/10.1186/s13068-018-1350-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6318881PMC
January 2019
1 Read

Enzymatic hydrolysis is limited by biomass-water interactions at high-solids: improved performance through substrate modifications.

Biotechnol Biofuels 2019 4;12. Epub 2019 Jan 4.

Department of Geosciences and Natural Resource Management, University of Copenhagen, Frederiksberg, Denmark.

Background: To improve process economics for production of fuels and chemicals from lignocellulosic biomass, high solids concentrations are applied in enzymatic hydrolysis, to increase product concentration and reduce energy input. However, increasing solids concentrations decrease cellulose conversion yields, the so called 'high-solids effect.' Previous work suggests that product inhibition and mixing contribute, but an understanding of how biomass properties influence the high-solids effect, is lacking. Read More

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http://dx.doi.org/10.1186/s13068-018-1339-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6318902PMC
January 2019
1 Read

Delineating the molecular responses of a halotolerant microalga using integrated omics approach to identify genetic engineering targets for enhanced TAG production.

Biotechnol Biofuels 2019 4;12. Epub 2019 Jan 4.

1Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand 247667 India.

Background: Harnessing the halotolerant characteristics of microalgae provides a viable alternative for sustainable biomass and triacylglyceride (TAG) production. sp. IITRIND2 is a fast growing fresh water microalga that has the capability to thrive in high saline environments. Read More

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http://dx.doi.org/10.1186/s13068-018-1343-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6318984PMC
January 2019
1 Read
6.044 Impact Factor

Sequential fractionation of the lignocellulosic components in hardwood based on steam explosion and hydrotropic extraction.

Biotechnol Biofuels 2019 4;12. Epub 2019 Jan 4.

Department of Chemical Engineering, Lund University, P.O. Box 124, 221 00 Lund, Sweden.

Background: The forest biorefinery plays an important part in the evolving circular bioeconomy due to its capacity to produce a portfolio of bio-based and sustainable fuels, chemicals, and materials. To tap into its true potential, more efficient and environmentally benign methods are needed to fractionate woody biomass into its main components (cellulose, hemicellulose, and lignin) without reducing their potential for valorization. This work presents a sequential fractionation method for hardwood based on steam pretreatment (STEX) and hydrotropic extraction (HEX) with sodium xylene sulfonate. Read More

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http://dx.doi.org/10.1186/s13068-018-1346-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6318938PMC
January 2019
5 Reads

Discovery of novel geranylgeranyl reductases and characterization of their substrate promiscuity.

Biotechnol Biofuels 2018 28;11:340. Epub 2018 Dec 28.

1Joint BioEnergy Institute, 5885 Hollis Street, 4th floor, Emeryville, CA 94608 USA.

Background: Geranylgeranyl reductase (GGR) is a flavin-containing redox enzyme that hydrogenates a variety of unactivated polyprenyl substrates, which are further processed mostly for lipid biosynthesis in archaea or chlorophyll biosynthesis in plants. To date, only a few GGR genes have been confirmed to reduce polyprenyl substrates in vitro or in vivo.

Results: In this work, we aimed to expand the confirmed GGR activity space by searching for novel genes that function under amenable conditions for microbial mesophilic growth in conventional hosts such as or 31 putative GGRs were selected to test for potential reductase activity in vitro on farnesyl pyrophosphate, geranylgeranyl pyrophosphate, farnesol (FOH), and geranylgeraniol (GGOH). Read More

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http://dx.doi.org/10.1186/s13068-018-1342-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6309074PMC
December 2018
6 Reads

Lipid metabolism of phenol-tolerant strains for lignin bioconversion.

Biotechnol Biofuels 2018 28;11:339. Epub 2018 Dec 28.

1Department of Energy, Environmental and Chemical Engineering, Washington University in St. Louis, St. Louis, MO 63130 USA.

Background: Lignin is a recalcitrant aromatic polymer that is a potential feedstock for renewable fuel and chemical production. PD630 is a promising strain for the biological upgrading of lignin due to its ability to tolerate and utilize lignin-derived aromatic compounds. To enhance its aromatic tolerance, we recently applied adaptive evolution using phenol as a sole carbon source and characterized a phenol-adapted strain (evol40) and the wild-type (WT) strain by whole genome and RNA sequencing. Read More

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http://dx.doi.org/10.1186/s13068-018-1337-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6309088PMC
December 2018
2 Reads
6.044 Impact Factor

Genomics and biochemistry investigation on the metabolic pathway of milled wood and alkali lignin-derived aromatic metabolites of SP-35.

Biotechnol Biofuels 2018 27;11:338. Epub 2018 Dec 27.

1Biofuels Institute, School of the Environment and Safety Engineering, Jiangsu University, Zhenjiang, Jiangsu China.

Background: The efficient depolymerization and utilization of lignin are one of the most important goals for the renewable use of lignocelluloses. The degradation and complete mineralization of lignin by bacteria represent a key step for carbon recycling in land ecosystems as well. However, many aspects of this process remain unclear, for example, the complex network of metabolic pathways involved in the degradation of lignin and the catabolic pathway of intermediate aromatic metabolites. Read More

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http://dx.doi.org/10.1186/s13068-018-1341-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6307125PMC
December 2018
1 Read

A process for purifying xylosugars of pre-hydrolysis liquor from kraft-based dissolving pulp production process.

Biotechnol Biofuels 2018 21;11:337. Epub 2018 Dec 21.

1State Key Laboratory of Bio-based Materials and Green Papermaking, Qilu University of Technology (Shandong Academy of Sciences), Jinan, 250353 Shandong China.

Background: In the kraft-based dissolving pulp production process, pre-hydrolysis liquor (PHL) is produced, which contains hemicelluloses, lignin, furfural and acetic acid. PHL is currently burned in the recovery boiler of the kraft pulping process, but it can be utilized for the generation of high-valued products, such as xylitol and xylanase, via fermentation processes. However, some PHL constituents, e. Read More

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http://dx.doi.org/10.1186/s13068-018-1336-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6302441PMC
December 2018
2 Reads
6.044 Impact Factor

Combined ensiling and hydrothermal processing as efficient pretreatment of sugarcane bagasse for 2G bioethanol production.

Biotechnol Biofuels 2018 20;11:336. Epub 2018 Dec 20.

1Center for BioProcess Engineering, Department of Chemical and Biochemical Engineering, Technical University of Denmark, DTU, Søltofts Plads 229, 2800 Kgs. Lyngby, Denmark.

Background: Ensiling cannot be utilized as a stand-alone pretreatment for sugar-based biorefinery processes but, in combination with hydrothermal processing, it can enhance pretreatment while ensuring a stable long-term storage option for abundant but moist biomass. The effectiveness of combining ensiling with hydrothermal pretreatment depends on biomass nature, pretreatment, and silage conditions.

Results: In the present study, the efficiency of the combined pretreatment was assessed by enzymatic hydrolysis and ethanol fermentation, and it was demonstrated that ensiling of sugarcane bagasse produces organic acids that can partly degrade biomass structure when in combination with hydrothermal treatment, with the consequent improvement of the enzymatic hydrolysis of cellulose and of the overall 2G bioethanol process efficiency. Read More

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https://biotechnologyforbiofuels.biomedcentral.com/articles/
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http://dx.doi.org/10.1186/s13068-018-1338-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6300893PMC
December 2018
2 Reads

Comparative transcriptome and metabolome analysis suggests bottlenecks that limit seed and oil yields in transgenic expressing diacylglycerol acyltransferase 1 and glycerol-3-phosphate dehydrogenase.

Biotechnol Biofuels 2018 19;11:335. Epub 2018 Dec 19.

Stockbridge School of Agriculture, University of Massachusetts, Amherst, MA 01003 USA.

Background: has attracted much interest as alternative renewable resources for biodiesel, other oil-based industrial products and a source for edible oils. Its unique oil attributes attract research to engineering new varieties of improved oil quantity and quality. The overexpression of enzymes catalyzing the synthesis of the glycerol backbone and the sequential conjugation of fatty acids into this backbone is a promising approach for increasing the levels of triacylglycerol (TAG). Read More

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http://dx.doi.org/10.1186/s13068-018-1326-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6299664PMC
December 2018
4 Reads

An NIRS-based assay of chemical composition and biomass digestibility for rapid selection of Jerusalem artichoke clones.

Biotechnol Biofuels 2018 19;11:334. Epub 2018 Dec 19.

1College of Agronomy and Biotechnology, China Agricultural University, Beijing, 100193 China.

Background: High-throughput evaluation of lignocellulosic biomass feedstock quality is the key to the successful commercialization of bioethanol production. Currently, wet chemical methods for the determination of chemical composition and biomass digestibility are expensive and time-consuming, thus hindering comprehensive feedstock quality assessments based on these biomass specifications. To find the ideal bioethanol feedstock, we perform a near-infrared spectroscopic (NIRS) assay to rapidly and comprehensively analyze the chemical composition and biomass digestibility of 59 Jerusalem artichoke ( L. Read More

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http://dx.doi.org/10.1186/s13068-018-1335-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6299672PMC
December 2018
1 Read

Comparative transcriptome analyses of oleaginous race A reveal significant differences in gene expression upon cobalt enrichment.

Biotechnol Biofuels 2018 18;11:333. Epub 2018 Dec 18.

2State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237 People's Republic of China.

Background: is known for its high hydrocarbon content, thus making it a strong candidate feedstock for biofuel production. Previous study has revealed that a high cobalt concentration can promote hydrocarbon synthesis and it has little effect on growth of cells. However, mechanisms beyond the cobalt enrichment remain unknown. Read More

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http://dx.doi.org/10.1186/s13068-018-1331-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6297975PMC
December 2018

Altering the linker in processive GH5 endoglucanase 1 modulates lignin binding and catalytic properties.

Biotechnol Biofuels 2018 18;11:332. Epub 2018 Dec 18.

The Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, Jiangsu Key Lab for the Chemistry & Utilization of Agricultural and Forest Biomass, College of Chemical Engineering, Nanjing Forestry University, Nanjing, 210037 Jiangsu China.

Background: The non-productive adsorption of cellulases onto lignin in biomass is a key issue for the biofuel process economy. It would be helpful to reduce the inhibitory effect of lignin on enzymatic hydrolysis by engineering weak lignin-binding cellulases. Cellulase linkers are highly divergent in their lengths, compositions, and glycosylations. Read More

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http://dx.doi.org/10.1186/s13068-018-1333-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6297974PMC
December 2018
1 Read
6.044 Impact Factor

Co-generation of ethanol and l-lactic acid from corn stalk under a hybrid process.

Biotechnol Biofuels 2018 18;11:331. Epub 2018 Dec 18.

1Fermentation Engineering Technology Research Center of Heibei Province, College of Bioscience & Bioengineering, Hebei University of Science and Technology, Shijiazhuang, 050000 People's Republic of China.

Background: Corn stover, as one important lignocellulosic material, has characteristics of low price, abundant output and easy availability. Using corn stover as carbon source in the fermentation of valuable organic chemicals contributes to reducing the negative environmental problems and the cost of production. In ethanol fermentation based on the hydrolysate of corn stover, the conversion rate of fermentable sugars is at a low level because the native does not utilize xylose. Read More

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http://dx.doi.org/10.1186/s13068-018-1330-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6298020PMC
December 2018
1 Read

Seed-specific suppression of ADP-glucose pyrophosphorylase in increases seed size and weight.

Biotechnol Biofuels 2018 18;11:330. Epub 2018 Dec 18.

1Department of Plant Sciences and Plant Pathology, Montana State University, Bozeman, MT 59717-3150 USA.

Background: Camelina ( L.) is a promising oilseed crop that may provide sustainable feedstock for biofuel production. One of the major drawbacks of Camelina is its smaller seeds compared to other major oil crops such as canola, which limit oil yield and may also pose challenges in successful seedling establishment, especially in dryland cultivation. Read More

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https://biotechnologyforbiofuels.biomedcentral.com/articles/
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http://dx.doi.org/10.1186/s13068-018-1334-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6297958PMC
December 2018
11 Reads

Integrating sugarcane molasses into sequential cellulosic biofuel production based on SSF process of high solid loading.

Biotechnol Biofuels 2018 18;11:329. Epub 2018 Dec 18.

College of Forestry and Landscape Architecture, Guangdong Engineering Technology Research Center of Agricultural and Forestry Biomass, Key Laboratory of Energy Plants Resource and Utilization, Ministry of Agriculture, South China Agricultural University, Guangzhou, 510642 People's Republic of China.

Background: Sugarcane bagasse (SCB) is one of the most promising lignocellulosic biomasses for use in the production of biofuels. However, bioethanol production from pure SCB fermentation is still limited by its high process cost and low fermentation efficiency. Sugarcane molasses, as a carbohydrate-rich biomass, can provide fermentable sugars for ethanol production. Read More

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http://dx.doi.org/10.1186/s13068-018-1328-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6297951PMC
December 2018
1 Read

Mechanism and enhancement of lipid accumulation in filamentous oleaginous microalgae under heterotrophic condition.

Biotechnol Biofuels 2018 11;11:328. Epub 2018 Dec 11.

1Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, 266101 Shandong People's Republic of China.

Background: The filamentous microalgae accumulates large amounts of lipids under photoautotrophic condition, while under heterotrophic condition, the lipid content decreased dramatically. Determination of the differences in metabolic pathways between photoautotrophic and heterotrophic growth will provide targets and strategies for improvement of lipid accumulation in heterotrophic cells.

Methods: The metabolic differences between photoautotrophically and heterotrophically cultivated cells were studied by comparing the growth, biochemical compositions and transcriptomic and metabolomic profiles of the cells. Read More

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http://dx.doi.org/10.1186/s13068-018-1329-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6290495PMC
December 2018
2 Reads

Correction to: Developing fast enzyme recycling strategy through elucidating enzyme adsorption kinetics on alkali and acid pretreated corn stover.

Biotechnol Biofuels 2018 10;11:327. Epub 2018 Dec 10.

School of Environmental and Biological Engineering, Nanjing University of Science and Technology, 200 Xiaolingwei Street, Nanjing, 210094 China.

[This corrects the article DOI: 10.1186/s13068-018-1315-5.]. Read More

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http://dx.doi.org/10.1186/s13068-018-1322-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6287345PMC
December 2018
1 Read

Cas9-guide RNA ribonucleoprotein-induced genome editing in the industrial green alga sp. strain KJ.

Biotechnol Biofuels 2018 10;11:326. Epub 2018 Dec 10.

2Research and Development Initiative, Chuo University, Bunkyo-ku, Tokyo, 112-8551 Japan.

Background: Oxygen-evolving photosynthetic microorganisms, collectively termed as microalgae, are gaining attention as alternative fuel sources. The unicellular alga sp. strain KJ that belongs to the class Trebouxiophyceae can grow rapidly in minimal mineral media and accumulate triacylglycerols at levels > 60% (w/w) of its dry weight under nitrogen depletion conditions. Read More

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http://dx.doi.org/10.1186/s13068-018-1327-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6287348PMC
December 2018
1 Read

In silico-designed lignin peroxidase from shows enhanced acid stability for depolymerization of lignin.

Biotechnol Biofuels 2018 10;11:325. Epub 2018 Dec 10.

1School of Energy and Chemical Engineering, UNIST, 50 UNIST-gil, Ulju-gun, Ulsan, 44919 Republic of Korea.

Background: The lignin peroxidase isozyme H8 from the white-rot fungus (LiPH8) demonstrates a high redox potential and can efficiently catalyze the oxidation of veratryl alcohol, as well as the degradation of recalcitrant lignin. However, native LiPH8 is unstable under acidic pH conditions. This characteristic is a barrier to lignin depolymerization, as repolymerization of phenolic products occurs simultaneously at neutral pH. Read More

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https://biotechnologyforbiofuels.biomedcentral.com/articles/
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http://dx.doi.org/10.1186/s13068-018-1324-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6287364PMC
December 2018
5 Reads
6.044 Impact Factor

Expression of an endoglucanase-cellobiohydrolase fusion protein in and .

Biotechnol Biofuels 2018 3;11:322. Epub 2018 Dec 3.

Biosciences Center, National Renewable Energy Laboratory, Golden, CO 80401 USA.

The low secretion levels of cellobiohydrolase I (CBHI) in yeasts are one of the key barriers preventing yeast from directly degrading and utilizing lignocellulose. To overcome this obstacle, we have explored the approach of genetically linking an easily secreted protein to CBHI, with CBHI being the last to be folded. The eg2 (EGII) gene was selected as the leading gene due to its previously demonstrated outstanding secretion in yeast. Read More

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http://dx.doi.org/10.1186/s13068-018-1301-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6278004PMC
December 2018
11 Reads

NO and CH emission from energy crop fields in the infertile Loess Plateau of China.

Biotechnol Biofuels 2018 3;11:321. Epub 2018 Dec 3.

1Key Laboratory of Plant Resources and Beijing Botanical Garden, Institute of Botany, Chinese Academy of Sciences, No. 20 Nanxincun, Xiangshan, Beijing, 100093 China.

Background: The greenhouse gas (GHG) mitigation is one of the most important environmental benefits of using bioenergy replacing fossil fuels. Nitrous oxide (NO) and methane (CH) are important GHGs and have drawn extra attention for their roles in global warming. Although there have been many works of soil emissions of NO and CH from bioenergy crops in the field scale, GHG emissions in large area of marginal lands are rather sparse and how soil temperature and moisture affect the emission potential remains unknown. Read More

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http://dx.doi.org/10.1186/s13068-018-1320-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6276234PMC
December 2018
8 Reads

A new approach to recycle oxalic acid during lignocellulose pretreatment for xylose production.

Biotechnol Biofuels 2018 5;11:324. Epub 2018 Dec 5.

1State Key Laboratory of Pulp and Paper Engineering, South China University of Technology, Guangzhou, 510640 China.

Background: Dilute oxalic acid pretreatment has drawn much attention because it could selectively hydrolyse the hemicellulose fraction during lignocellulose pretreatment. However, there are few studies focusing on the recovery of oxalic acid. Here, we reported a new approach to recycle oxalic acid used in pretreatment via ethanol extraction. Read More

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http://dx.doi.org/10.1186/s13068-018-1325-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6280388PMC
December 2018
8 Reads

Metabolic engineering of the thermophilic filamentous fungus to produce fumaric acid.

Biotechnol Biofuels 2018 3;11:323. Epub 2018 Dec 3.

2Key Laboratory of Systems Microbial Biotechnology, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308 China.

Background: Fumaric acid is widely used in food and pharmaceutical industries and is recognized as a versatile industrial chemical feedstock. Increasing concerns about energy and environmental problems have resulted in a focus on fumaric acid production by microbial fermentation via bioconversion of renewable feedstocks. Filamentous fungi are the predominant microorganisms used to produce organic acids, including fumaric acid, and most studies to date have focused on species. Read More

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https://biotechnologyforbiofuels.biomedcentral.com/articles/
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http://dx.doi.org/10.1186/s13068-018-1319-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6278111PMC
December 2018
9 Reads

Simultaneous consumption of cellobiose and xylose by to circumvent glucose repression and identification of its cellobiose-assimilating operons.

Biotechnol Biofuels 2018 1;11:320. Epub 2018 Dec 1.

1Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, Nanjing Forestry University, Nanjing, 210037 People's Republic of China.

Background: The use of inedible lignocellulosic biomasses for biomanufacturing provides important environmental and economic benefits for society. Efficient co-utilization of lignocellulosic biomass-derived sugars, primarily glucose and xylose, is critical for the viability of lignocellulosic biorefineries. However, the phenomenon of glucose repression prevents co-utilization of both glucose and xylose in cellulosic hydrolysates. Read More

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http://dx.doi.org/10.1186/s13068-018-1323-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6271610PMC
December 2018
7 Reads

Cellulose-specific Type B carbohydrate binding modules: understanding oligomeric and non-crystalline substrate recognition mechanisms.

Biotechnol Biofuels 2018 30;11:319. Epub 2018 Nov 30.

Department of Chemical and Materials Engineering, University of Kentucky, 177 F Paul Anderson Tower, Lexington, KY 40506 USA.

Background: Effective enzymatic degradation of crystalline polysaccharides requires a synergistic cocktail of hydrolytic enzymes tailored to the wide-ranging degree of substrate crystallinity. To accomplish this type of targeted carbohydrate recognition, nature produces multi-modular enzymes, having at least one catalytic domain appended to one or more carbohydrate binding modules (CBMs). The Type B CBM categorization encompasses several families (i. Read More

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http://dx.doi.org/10.1186/s13068-018-1321-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6267901PMC
November 2018
4 Reads

Dual expression of plastidial GPAT1 and LPAT1 regulates triacylglycerol production and the fatty acid profile in .

Biotechnol Biofuels 2018 22;11:318. Epub 2018 Nov 22.

1Key Laboratory of Eutrophication and Red Tide Prevention of Guangdong Higher Education Institutes, College of Life Science, Jinan University, Guangzhou, 510632 China.

Background: Metabolic engineering has emerged as a potential strategy for improving microalgal lipid content through targeted changes to lipid metabolic networks. However, the intricate nature of lipogenesis has impeded metabolic engineering. Therefore, it is very important to identify the crucial metabolic nodes and develop strategies to exploit multiple genes for transgenesis. Read More

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https://biotechnologyforbiofuels.biomedcentral.com/articles/
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http://dx.doi.org/10.1186/s13068-018-1317-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6249879PMC
November 2018
8 Reads

Real-time adsorption and action of expansin on cellulose.

Biotechnol Biofuels 2018 22;11:317. Epub 2018 Nov 22.

1State Key Laboratory of Chemical Engineering, Tianjin Key Laboratory of Membrane Science and Desalination Technology, School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072 China.

Background: Biological pretreatment is an environmentally safe method for disrupting recalcitrant structures of lignocellulose and thereby improving their hydrolysis efficiency. Expansin and expansin-like proteins act synergistically with cellulases during hydrolysis. A systematic analysis of the adsorption behavior and mechanism of action of expansin family proteins can provide a basis for the development of highly efficient pretreatment methods for cellulosic substrates using expansins. Read More

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https://biotechnologyforbiofuels.biomedcentral.com/articles/
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http://dx.doi.org/10.1186/s13068-018-1318-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6249958PMC
November 2018
16 Reads

Developing fast enzyme recycling strategy through elucidating enzyme adsorption kinetics on alkali and acid pretreated corn stover.

Biotechnol Biofuels 2018 20;11:316. Epub 2018 Nov 20.

School of Environmental and Biological Engineering, Nanjing University of Science and Technology, 200 Xiaolingwei Street, Nanjing, 210094 China.

Background: Although various pre-treatment methods have been developed to disrupt the structure of lignocellulosic biomass, high dosage of cellulases is still required to hydrolyze lignocellulose to fermentable sugars. Enzyme recycling via recycling unhydrolyzed solids after enzymatic hydrolysis is a promising strategy to reduce enzyme loading for production of cellulosic ethanol.

Results: To develop effective enzyme recycling method via recycling unhydrolyzed solids, this work investigated both enzymatic hydrolysis kinetics and enzyme adsorption kinetics on dilute acid and dilute alkali pre-treated corn stover (CS). Read More

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http://dx.doi.org/10.1186/s13068-018-1315-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6245881PMC
November 2018
2 Reads

grows vegetatively in a biofilm rich in heteropolysaccharides and cytoplasmic proteins.

Biotechnol Biofuels 2018 20;11:315. Epub 2018 Nov 20.

1State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, No. 30, Puzhu South Road, Nanjing, 211800 China.

Background: Biofilms are cell communities wherein cells are embedded in a self-produced extracellular polymeric substances (EPS). The biofilm of confers the cells superior phenotypes and has been extensively exploited to produce a variety of liquid biofuels and bulk chemicals. However, little has been known about the physiology of in biofilm as well as the composition and biosynthesis of the EPS. Read More

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https://biotechnologyforbiofuels.biomedcentral.com/articles/
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http://dx.doi.org/10.1186/s13068-018-1316-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6245871PMC
November 2018
11 Reads

The putative β-glucosidase BGL3I regulates cellulase induction in .

Biotechnol Biofuels 2018 19;11:314. Epub 2018 Nov 19.

1CAS-Key Laboratory of Synthetic Biology, CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 300 Fenglin Rd, Shanghai, 200032 China.

Background: The filamentous fungus (anamorph of ) displays increased cellulase expression while growing on inducers such as lactose or cellulose. However, the mechanism of cellulase induction in is not yet completely characterized. Here, a protein annotated as β-glucosidase (BGL3I) was found to be involved in cellulase induction in . Read More

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http://dx.doi.org/10.1186/s13068-018-1314-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6240962PMC
November 2018
2 Reads
6.044 Impact Factor

Retraction Note to: Bacterial conversion of depolymerized Kraft lignin.

Biotechnol Biofuels 2018 12;11:313. Epub 2018 Nov 12.

1Department of Chemical Engineering, Lund University, P.O. Box 124, 221 00 Lund, Sweden.

[This retracts the article DOI: 10.1186/s13068-018-1240-7.]. Read More

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http://dx.doi.org/10.1186/s13068-018-1307-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6233592PMC
November 2018
2 Reads