1,814 results match your criteria Biotechnology for Biofuels [Journal]


Assessing the stability and techno-economic implications for wet storage of harvested microalgae to manage seasonal variability.

Biotechnol Biofuels 2019 8;12:80. Epub 2019 Apr 8.

3Arizona State University, Mesa, AZ 85212 USA.

Background: Seasonal variation in microalgae production is a significant challenge to developing cost-competitive algae biofuels. Summer production can be three to five times greater than winter production, which could result in winter biomass shortages and summer surpluses at algae biorefineries. While the high water content (80%, wet basis) of harvested microalgae biomass makes drying an expensive approach to preservation, it is not an issue for ensiling. Read More

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http://dx.doi.org/10.1186/s13068-019-1420-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6452513PMC

Biochemical characterization of three new α-olefin-producing P450 fatty acid decarboxylases with a halophilic property.

Biotechnol Biofuels 2019 8;12:79. Epub 2019 Apr 8.

1Shandong Provincial Key Laboratory of Synthetic Biology, CAS Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, No. 189 Songling Road, Qingdao, 266101 Shandong China.

Background: The CYP152 family member OleT from sp. ATCC 8456 has been well-known to catalyze the unusual one-step decarboxylation of free fatty acids towards the formation of terminal alkenes. Efforts to tune up its decarboxylation activity for better production of biological alkenes have been extensively explored via approaches such as site-directed mutagenesis and electron source engineering, but with limited success. Read More

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http://dx.doi.org/10.1186/s13068-019-1419-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6452516PMC

Direct enzymatic ethanolysis of potential biomass for co-production of sustainable biodiesel and nutraceutical eicosapentaenoic acid.

Biotechnol Biofuels 2019 5;12:78. Epub 2019 Apr 5.

1BIC-ESAT, College of Engineering, Peking University, Beijing, 100871 China.

Background: Marine microalga is a promising source for the production of renewable and sustainable biodiesel in replacement of depleting petroleum. Other than biodiesel, is a green and potential resource for the commercial production of nutraceutical eicosapentaenoic acid (EPA, C). In recent studies, low-value biodiesel can be achieved by transesterification of biomass. Read More

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https://biotechnologyforbiofuels.biomedcentral.com/articles/
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http://dx.doi.org/10.1186/s13068-019-1418-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6449970PMC
April 2019
1 Read

Moulding the mould: understanding and reprogramming filamentous fungal growth and morphogenesis for next generation cell factories.

Biotechnol Biofuels 2019 2;12:77. Epub 2019 Apr 2.

1Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308 China.

Filamentous fungi are harnessed as cell factories for the production of a diverse range of organic acids, proteins, and secondary metabolites. Growth and morphology have critical implications for product titres in both submerged and solid-state fermentations. Recent advances in systems-level understanding of the filamentous lifestyle and development of sophisticated synthetic biological tools for controlled manipulation of fungal genomes now allow rational strain development programs based on data-driven decision making. Read More

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http://dx.doi.org/10.1186/s13068-019-1400-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6446404PMC

Tracking of enzymatic biomass deconstruction by fungal secretomes highlights markers of lignocellulose recalcitrance.

Biotechnol Biofuels 2019 1;12:76. Epub 2019 Apr 1.

2INRA, Aix Marseille Univ., UMR1163, BBF, Biodiversité et Biotechnologie Fongiques, Marseille, France.

Background: Lignocellulose biomass is known as a recalcitrant material towards enzymatic hydrolysis, increasing the process cost in biorefinery. In nature, filamentous fungi naturally degrade lignocellulose, using an arsenal of hydrolytic and oxidative enzymes. Assessment of enzyme hydrolysis efficiency generally relies on the yield of glucose for a given biomass. Read More

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http://dx.doi.org/10.1186/s13068-019-1417-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6442405PMC
April 2019
1 Read

Degradation of bamboo lignocellulose by bamboo snout beetle in vivo and vitro: efficiency and mechanism.

Biotechnol Biofuels 2019 1;12:75. Epub 2019 Apr 1.

1Bamboo Diseases and Pests Control and Resources Development Key Laboratory of Sichuan Province, Leshan Normal University, No. 778, Riverside Road, Central District, Leshan, 614000 China.

Background: As an important biomass raw material, the lignocellulose in bamboo is of significant value in energy conversion. The conversion of bamboo lignocellulose into fermentable reducing sugar, i.e. Read More

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http://dx.doi.org/10.1186/s13068-019-1406-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6442404PMC
April 2019
1 Read

Expression and secretion of a lytic polysaccharide monooxygenase by a fast-growing cyanobacterium.

Biotechnol Biofuels 2019 1;12:74. Epub 2019 Apr 1.

1Copenhagen Plant Science Centre, Department of Plant and Environmental Sciences, University of Copenhagen, Frederiksberg C, Denmark.

Background: Cyanobacteria have the potential to become next-generation cell factories due to their ability to use CO, light and inorganic nutrients to produce a range of biomolecules of commercial interest. UTEX 2973, in particular, is a fast-growing, genetically tractable, cyanobacterium that has garnered attention as a potential biotechnological chassis. To establish this unique strain as a host for heterologous protein production, we aimed to demonstrate expression and secretion of the industrially relevant AA10A, a lytic polysaccharide monooxygenase from the Gram-positive bacterium

Results: Two variations of AA10A were successfully expressed in UTEX 2973: One containing the native N-terminal, Sec-targeted, signal peptide and a second with a Tat-targeted signal peptide from the trimethylamine--oxide reductase (TorA). Read More

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http://dx.doi.org/10.1186/s13068-019-1416-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6442416PMC

Identification of functional butanol-tolerant genes from mutants derived from error-prone PCR-based whole-genome shuffling.

Biotechnol Biofuels 2019 1;12:73. Epub 2019 Apr 1.

1Biomass Conversion Laboratory, R&D Center for Petrochemical Technology, Tianjin University, Tianjin, 300072 People's Republic of China.

Background: Butanol is an important biofuel and chemical. The development of butanol-tolerant strains and the identification of functional butanol-tolerant genes is essential for high-yield bio-butanol production due to the toxicity of butanol.

Results: BW25113 was subjected for the first time to error-prone PCR-based whole-genome shuffling. Read More

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https://biotechnologyforbiofuels.biomedcentral.com/articles/
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http://dx.doi.org/10.1186/s13068-019-1405-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6442406PMC
April 2019
6 Reads

Less biomass and intracellular glutamate in anodic biofilms lead to efficient electricity generation by microbial fuel cells.

Biotechnol Biofuels 2019 1;12:72. Epub 2019 Apr 1.

1Graduate School of Science, Technology and Innovation, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe, Hyogo 657-8501 Japan.

Background: Using a microbial fuel cell (MFC), we observed that a complex microbial community decomposed starch and transferred electrons to a graphite felt anode to generate current. In spite of the same reactor configuration, inoculum, substrate, temperature, and pH, MFCs produced different current and power density. To understand which factor(s) affected electricity generation, here, we analyzed a complex microbial community in an anodic biofilm and fermentation broth using Illumina MiSeq sequencing and metabolomics. Read More

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http://dx.doi.org/10.1186/s13068-019-1414-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6442422PMC

MoC-induced hydrogen production enhances microbial electrosynthesis of acetate from CO reduction.

Biotechnol Biofuels 2019 1;12:71. Epub 2019 Apr 1.

1State Key Laboratory of Materials-Oriented Chemical Engineering, Nanjing Tech University, Nanjing, 211816 People's Republic of China.

Background: Microbial electrosynthesis (MES) is a biocathode-driven process, in which electroautotrophic microorganisms can directly uptake electrons or indirectly via H from the cathode as energy sources and CO as only carbon source to produce chemicals.

Results: This study demonstrates that a hydrogen evolution reaction (HER) catalyst can enhance MES performance. An active HER electrocatalyst molybdenum carbide (MoC)-modified electrode was constructed for MES. Read More

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http://dx.doi.org/10.1186/s13068-019-1413-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6442412PMC
April 2019
4 Reads

Bamboo lignocellulose degradation by gut symbiotic microbiota of the bamboo snout beetle .

Biotechnol Biofuels 2019 1;12:70. Epub 2019 Apr 1.

1Bamboo Diseases and Pests Control and Resources Development Key Laboratory of Sichuan Province, Leshan Normal University, No. 778, Riverside Road, Central District, Leshan, 614000 Sichuan China.

Background: Gut symbiotic microbiota plays a critical role in nutrient supply, digestion, and absorption. The bamboo snout beetle, , a common pest of several bamboo species, exhibits high lignocellulolytic enzyme activity and contains various CAZyme genes. However, to date, no studies have evaluated the role of gut symbiotic microbiota of the snout beetle on bamboo lignocellulose degradation. Read More

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https://biotechnologyforbiofuels.biomedcentral.com/articles/
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http://dx.doi.org/10.1186/s13068-019-1411-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6442426PMC
April 2019
2 Reads

Comprehensive evaluation of a cost-effective method of culturing with unsterilized piggery wastewater for biofuel production.

Biotechnol Biofuels 2019 1;12:69. Epub 2019 Apr 1.

3School of Animal Rural and Environmental Sciences, Nottingham Trent University, Brackenhurst, Southwell, Nottinghamshire NG25 0QF UK.

Background: The utilization of for the dual goals of biofuel production and wastewater nutrient removal is highly attractive. Moreover, this technology combined with flue gas (rich in CO) cleaning is considered to be an effective way of improving biofuel production. However, the sterilization of wastewater is an energy-consuming step. Read More

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http://dx.doi.org/10.1186/s13068-019-1407-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6442423PMC
April 2019
1 Read

Understanding the functions of endogenous DOF transcript factor in .

Biotechnol Biofuels 2019 27;12:67. Epub 2019 Mar 27.

Guangdong Engineering Research Center for Marine Algal Biotechnology, Guangdong Key Laboratory of Plant Epigenetics, Shenzhen Key Laboratory of Marine Bioresource and Eco-environmental Science, Longhua Innovation Institute for Biotechnology, College of Life Sciences, Shenzhen University, Shenzhen, 518060 People's Republic of China.

Background: The regulation of genes related to lipid metabolism by genetic engineering is an important way to increase the accumulation of lipids in microalgae. DNA binding with one finger (DOF) is a plant-specific transcription factor in higher plants, where it regulates carbon and nitrogen metabolic pathways by regulating key genes involved in these pathways. Overexpression of DOF can increase lipid production in plants; however, it is not clear whether overexpression of DOF can increase lipids in microalgae. Read More

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http://dx.doi.org/10.1186/s13068-019-1403-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6436238PMC

Improving squalene production by enhancing the NADPH/NADP ratio, modifying the isoprenoid-feeding module and blocking the menaquinone pathway in .

Biotechnol Biofuels 2019 28;12:68. Epub 2019 Mar 28.

The Molecular Virology and Viral Immunology Laboratory, Xi'an Medical University, Xi'an, 710021 Shaanxi China.

Background: Squalene is currently used widely in the food, cosmetics, and medicine industries. It could also replace petroleum as a raw material for fuels. Microbial fermentation processes for squalene production have been emerging over recent years. Read More

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http://dx.doi.org/10.1186/s13068-019-1415-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6437923PMC
March 2019
6.044 Impact Factor

CRISPR-Cas ribonucleoprotein mediated homology-directed repair for efficient targeted genome editing in microalgae IMET1.

Biotechnol Biofuels 2019 25;12:66. Epub 2019 Mar 25.

1Laboratory of Microbiology, Wageningen University, Stippeneng 4, 6708 PD Wageningen, The Netherlands.

Background: Microalgae are considered as a sustainable feedstock for the production of biofuels and other value-added compounds. In particular, spp. stand out from other microalgal species due to their capabilities to accumulate both triacylglycerol (TAG) and polyunsaturated fatty acids (PUFAs). Read More

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http://dx.doi.org/10.1186/s13068-019-1401-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6432748PMC

Modular systems metabolic engineering enables balancing of relevant pathways for l-histidine production with .

Biotechnol Biofuels 2019 25;12:65. Epub 2019 Mar 25.

1Institute of Biochemical Engineering, University of Stuttgart, Allmandring 31, 70569 Stuttgart, Germany.

Background: l-Histidine biosynthesis is embedded in an intertwined metabolic network which renders microbial overproduction of this amino acid challenging. This is reflected in the few available examples of histidine producers in literature. Since knowledge about the metabolic interplay is limited, we systematically perturbed the metabolism of to gain a holistic understanding in the metabolic limitations for l-histidine production. Read More

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http://dx.doi.org/10.1186/s13068-019-1410-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6432763PMC

Insight into the role of α-arabinofuranosidase in biomass hydrolysis: cellulose digestibility and inhibition by xylooligomers.

Biotechnol Biofuels 2019 22;12:64. Epub 2019 Mar 22.

College of Forestry, Northwest A&F University, 3 Taicheng Road, Yangling, 712100 Shaanxi China.

Background: α-l-Arabinofuranosidase (ARA), a debranching enzyme that can remove arabinose substituents from arabinoxylan and arabinoxylooligomers (AXOS), promotes the hydrolysis of the arabinoxylan fraction of biomass; however, the impact of ARA on the overall digestibility of cellulose is controversial. In this study, we investigated the effects of the addition of ARA on cellulase hydrolytic action.

Results: We found that approximately 15% of the xylan was converted into AXOS during the hydrolysis of aqueous ammonia-pretreated corn stover and that this AXOS fraction was approximately 12% substituted with arabinose. Read More

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http://dx.doi.org/10.1186/s13068-019-1412-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6429694PMC
March 2019
1 Read

developing ethanol tolerance during adaptive evolution with significant improvements of multiple pathways.

Biotechnol Biofuels 2019 22;12:63. Epub 2019 Mar 22.

1State Key Laboratory of Genetic Engineering, School of Life Science, Fudan University, Shanghai, 200438 China.

Background: , the known fastest-growing eukaryote on the earth, has remarkable thermotolerance and capacity to utilize various agricultural residues to produce low-cost bioethanol, and hence is industrially important to resolve the imminent energy shortage crisis. Currently, the poor ethanol tolerance hinders its operable application in the industry, and it is necessary to improve ' ethanol resistance and unravel the underlying systematical mechanisms. However, this has been seldom reported to date. Read More

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https://biotechnologyforbiofuels.biomedcentral.com/articles/
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http://dx.doi.org/10.1186/s13068-019-1393-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6429784PMC
March 2019
8 Reads

Metabolic engineering of for the production of butyric acid at high titer and productivity.

Biotechnol Biofuels 2019 22;12:62. Epub 2019 Mar 22.

1Department of Microbiology and Cell Science, University of Florida, Gainesville, FL 32611 USA.

Background: Several anaerobic bacteria produce butyric acid, a commodity chemical with use in chemical, pharmaceutical, food and feed industries, using complex media with acetate as a co-product. Butyrate titer of various recombinant did not exceed 10 g l in batch fermentations in any of the media tested.

Results: A recombinant (strain LW393) that produced butyrate as the major fermentation product was constructed with genes from , and . Read More

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https://biotechnologyforbiofuels.biomedcentral.com/articles/
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http://dx.doi.org/10.1186/s13068-019-1408-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6429758PMC
March 2019
1 Read

Modulation of acetate utilization in by metabolic engineering of tolerance and metabolism.

Biotechnol Biofuels 2019 21;12:61. Epub 2019 Mar 21.

1State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237 China.

Background: Acetate, an economical industrial chemical, which is also the precursor of acetyl-CoA, could serve as an alternative substrate for biomanufacturing. This nontraditional substrate can be widely produced from syngas via hydrolysis or pyrolysis of the cellulosic biomass, chemical or microbial catalysis, anaerobic fermentation in treated wastewater, etc. However, the toxicity of acetate to microorganisms has held back its utilization, especially for the eukaryotes that are good hosts for production of complicated pharmaceuticals or chemicals. Read More

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http://dx.doi.org/10.1186/s13068-019-1404-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6427870PMC

Discovery of potential pathways for biological conversion of poplar wood into lipids by co-fermentation of strains.

Biotechnol Biofuels 2019 19;12:60. Epub 2019 Mar 19.

1Bioproducts, Sciences and Engineering Laboratory, Department of Biological Systems Engineering, Washington State University, 2710 Crimson Way, Richland, WA 99354 USA.

Background: Biological routes for utilizing both carbohydrates and lignin are important to reach the ultimate goal of bioconversion of full carbon in biomass into biofuels and biochemicals. Recent biotechnology advances have shown promises toward facilitating biological transformation of lignin into lipids. Natural and engineered  strains (e. Read More

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http://dx.doi.org/10.1186/s13068-019-1395-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6423811PMC

QTL analysis reveals genomic variants linked to high-temperature fermentation performance in the industrial yeast.

Biotechnol Biofuels 2019 19;12:59. Epub 2019 Mar 19.

1CAS Key Laboratory of Systems Microbial Biotechnology, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308 China.

Background: High-temperature fermentation is desirable for the industrial production of ethanol, which requires thermotolerant yeast strains. However, yeast thermotolerance is a complicated quantitative trait. The understanding of genetic basis behind high-temperature fermentation performance is still limited. Read More

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http://dx.doi.org/10.1186/s13068-019-1398-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6423876PMC
March 2019
1 Read
6.044 Impact Factor

On the functional characterization of lytic polysaccharide monooxygenases (LPMOs).

Biotechnol Biofuels 2019 19;12:58. Epub 2019 Mar 19.

Faculty of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences (NMBU), PO Box 5003, 1432 Ås, Norway.

Lytic polysaccharide monooxygenases (LPMOs) are abundant in nature and best known for their role in the enzymatic conversion of recalcitrant polysaccharides such as chitin and cellulose. LPMO activity requires an oxygen co-substrate, which was originally thought to be O, but which may also be HO. Functional characterization of LPMOs is not straightforward because typical reaction mixtures will promote side reactions, including auto-catalytic inactivation of the enzyme. Read More

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http://dx.doi.org/10.1186/s13068-019-1392-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6423801PMC
March 2019
2 Reads

New strategy to elucidate the positive effects of extractable lignin on enzymatic hydrolysis by quartz crystal microbalance with dissipation.

Biotechnol Biofuels 2019 19;12:57. Epub 2019 Mar 19.

1Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, Nanjing Forestry University, Nanjing, 210037 China.

Background: The presence of lignin normally affects enzymatic saccharification of lignocellulose detrimentally. However, positive effects of lignin on enzymatic hydrolysis have been recently reported. Enzyme-lignin interactions could be the key to reveal the underlying mechanism of their discrepant behaviors. Read More

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http://dx.doi.org/10.1186/s13068-019-1402-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6423845PMC

Bacterial conversion of depolymerized Kraft lignin.

Biotechnol Biofuels 2019 16;12:56. Epub 2019 Mar 16.

1Department of Chemical Engineering, Lund University, P.O. Box 124, 221 00 Lund, Sweden.

Background: Lignin is a potential feedstock for microbial conversion into various chemicals. However, the microbial degradation rate of native or technical lignin is low, and chemical depolymerization is needed to obtain reasonable conversion rates. In the current study, nine bacterial strains belonging to the and genera were evaluated for their ability to grow on alkaline-treated softwood lignin as a sole carbon source. Read More

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http://dx.doi.org/10.1186/s13068-019-1397-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6420747PMC

AA16, a new lytic polysaccharide monooxygenase family identified in fungal secretomes.

Biotechnol Biofuels 2019 16;12:55. Epub 2019 Mar 16.

1Biodiversité et Biotechnologie Fongiques, UMR1163, INRA, Aix Marseille Université, Marseille, France.

Background: Lignocellulosic biomass is considered as a promising alternative to fossil resources for the production of fuels, materials and chemicals. Efficient enzymatic systems are needed to degrade the plant cell wall and overcome its recalcitrance. A widely used producer of cellulolytic cocktails is the ascomycete , but this organism secretes a limited set of enzymes. Read More

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http://dx.doi.org/10.1186/s13068-019-1394-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6420742PMC

Hydrodynamic performance of floating photobioreactors driven by wave energy.

Biotechnol Biofuels 2019 16;12:54. Epub 2019 Mar 16.

3State Key Laboratory of Biotechnology, East China University of Science and Technology, Shanghai, 200237 China.

Background: Unlike conventional cultivation systems, liquid mixing in floating photobioreactors (PBRs) is solely induced by their hydrodynamic movement in response to waves, and this movement is affected by the wave conditions (wave height and wave period), the PBR configuration and the culture depth. However, to the best of our knowledge, a practical study of the hydrodynamic movements of PBRs has not been previously conducted.

Results: This study aims to investigate the hydrodynamic performance of floating PBRs in response to wave conditions. Read More

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http://dx.doi.org/10.1186/s13068-019-1396-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6420745PMC

Filamentous cyanobacteria triples oil production in seawater-based medium supplemented with industrial waste: monosodium glutamate residue.

Biotechnol Biofuels 2019 14;12:53. Epub 2019 Mar 14.

1School of Environmental Science and Engineering, Shandong University, No.27 Shanda Nan Road, Jinan, 250100 China.

Background: To overcome the daunting technical and economic barriers of algal biofuels, we evaluated whether seawater can be a viable medium for economically producing filamentous as feedstock, using monosodium glutamate residue (MSGR) produced by the glutamate extraction process as an inexpensive nutrient source.

Results: cannot grow in pure seawater, but exhibited faster biomass accumulation in seawater supplemented with MSGR than in freshwater medium (modified Zarrouk medium). Introducing seawater into media ensured this cyanobacterium obtained high lipid productivity (120 mg/L/day) and suffered limited bacterial infections during growth. Read More

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http://dx.doi.org/10.1186/s13068-019-1391-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6417114PMC

Prediction and characterization of promoters and ribosomal binding sites of in system biology era.

Biotechnol Biofuels 2019 14;12:52. Epub 2019 Mar 14.

1State Key Laboratory of Biocatalysis and Enzyme Engineering, Hubei Collaborative Innovation Center for Green Transformation of Bio-resources, Environmental Microbial Technology Center of Hubei Province, and School of Life Sciences, Hubei University, Wuhan, 430062 China.

Background: is a model bacterial ethanologen with many systems biology studies reported. Besides lignocellulosic ethanol production, has been developed as a platform for biochemical production through metabolic engineering. However, identification and rigorous understanding of the genetic origins of cellular function, especially those based in non-coding region of DNA, such as promoters and ribosomal binding sites (RBSs), are still in its infancy. Read More

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https://biotechnologyforbiofuels.biomedcentral.com/articles/
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http://dx.doi.org/10.1186/s13068-019-1399-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6417218PMC
March 2019
5 Reads

Promoting enzymatic hydrolysis of lignocellulosic biomass by inexpensive soy protein.

Biotechnol Biofuels 2019 13;12:51. Epub 2019 Mar 13.

3Department of Sustainable Biomaterials, Virginia Tech, 230 Cheatham Hall, Blacksburg, VA 24060 USA.

Background: Liquid hot water (LHW) pretreatment has been considered as one of the most industrially viable and environment-friendly methods for facilitating the transformation of lignocelluloses into biofuels through biological conversion. However, lignin fragments in pretreatment hydrolysates are preferential to condense with each other and then deposit back onto cellulose surface under severe conditions. Particularly, lignin tends to relocate or redistribute under high-temperature LHW pretreatment conditions. Read More

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http://dx.doi.org/10.1186/s13068-019-1387-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6417190PMC
March 2019
2 Reads
6.044 Impact Factor

Metabolic engineering of to improve glycerol metabolism and furfural tolerance.

Biotechnol Biofuels 2019 9;12:50. Epub 2019 Mar 9.

1Department of Animal Sciences and Ohio State Agricultural Research and Development Center (OARDC), The Ohio State University, 305 Gerlaugh Hall, 1680 Madison Avenue, Wooster, OH 44691 USA.

Background: Inefficient utilization of glycerol by () is a major impediment to adopting glycerol metabolism as a strategy for increasing NAD(P)H regeneration, which would in turn, alleviate the toxicity of lignocellulose-derived microbial inhibitory compounds (LDMICs, e.g., furfural), and improve the fermentation of lignocellulosic biomass hydrolysates (LBH) to butanol. Read More

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http://dx.doi.org/10.1186/s13068-019-1388-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6408787PMC

Proteomic profiling and integrated analysis with transcriptomic data bring new insights in the stress responses of after an arrest during high-temperature ethanol fermentation.

Biotechnol Biofuels 2019 9;12:49. Epub 2019 Mar 9.

1MOST-USDA Joint Research Center for Biofuels, Beijing Engineering Research Center for Biofuels, Institute of New Energy Technology, Tsinghua University, Beijing, 100084 China.

Background: The thermotolerant yeast is a potential candidate for high-temperature fermentation. When was used for high-temperature ethanol fermentation, a fermentation arrest was observed during the late fermentation stage and the stress responses have been investigated based on the integration of RNA-Seq and metabolite data. In order to bring new insights into the cellular responses of after the fermentation arrest during high-temperature ethanol fermentation, quantitative proteomic profiling and integrated analysis with transcriptomic data were performed in this study. Read More

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http://dx.doi.org/10.1186/s13068-019-1390-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6408782PMC
March 2019
6.044 Impact Factor

A novel thermostable GH10 xylanase with activities on a wide variety of cellulosic substrates from a xylanolytic strain exhibiting significant synergy with commercial Celluclast 1.5 L in pretreated corn stover hydrolysis.

Biotechnol Biofuels 2019 9;12:48. Epub 2019 Mar 9.

College of Fundamental Medical Science, Guangzhou University of Chinese Medicine, Guangzhou Higher Education Mega Center, No.232 Outer Ring West Rd., Panyu District, Guangzhou, 510006 Guangdong China.

Background: Cellulose and hemicellulose are the two largest components in lignocellulosic biomass. Enzymes with activities towards cellulose and xylan have attracted great interest in the bioconversion of lignocellulosic biomass, since they have potential in improving the hydrolytic performance and reducing the enzyme costs. Exploring glycoside hydrolases (GHs) with good thermostability and activities on xylan and cellulose would be beneficial to the industrial production of biofuels and bio-based chemicals. Read More

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http://dx.doi.org/10.1186/s13068-019-1389-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6408826PMC
March 2019
1 Read

Butanol production from laccase-pretreated brewer's spent grain.

Biotechnol Biofuels 2019 5;12:47. Epub 2019 Mar 5.

Biopox srl, Via Salita Arenella 9, Naples, Italy.

Background: Beer is the most popular alcoholic beverage worldwide. In the manufacture of beer, various by-products and residues are generated, and the most abundant (85% of total by-products) are spent grains. Thanks to its high (hemi)cellulose content (about 50% w/w dry weight), this secondary raw material is attractive for the production of second-generation biofuels as butanol through fermentation processes. Read More

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http://dx.doi.org/10.1186/s13068-019-1383-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6399911PMC
March 2019
2 Reads

Deciphering optimal biostimulation strategy of supplementing anthocyanin-abundant plant extracts for bioelectricity extraction in microbial fuel cells.

Biotechnol Biofuels 2019 1;12:46. Epub 2019 Mar 1.

2Department of Chemical and Materials Engineering, National I-Lan University, Yilan, 26047 Taiwan.

Background: Microbial fuel cells (MFCs) are effective biofuel devices that use indigenous microbes to directly convert chemical energy from organics oxidation into bioelectric energy. To maximize energy-converting efficiency for bioelectricity generation in MFCs, redox mediators (RMs) (e.g. Read More

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http://dx.doi.org/10.1186/s13068-019-1385-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6396463PMC

Seed culture pre-adaptation of MA-13 improves lactic acid production in simultaneous saccharification and fermentation.

Biotechnol Biofuels 2019 28;12:45. Epub 2019 Feb 28.

2Division of Industrial Biotechnology, Department of Biology and Biological Engineering, Chalmers University of Technology, 412 96 Gothenburg, Sweden.

Background: Lignocellulosic biomass is an abundant and sustainable feedstock, which represents a promising raw material for the production of lactic acid via microbial fermentation. However, toxic compounds that affect microbial growth and metabolism are released from the biomass upon thermochemical pre-treatment. So far, susceptibility of bacterial strains to biomass-derived inhibitors still represents a major barrier to lactic acid production from lignocellulose. Read More

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https://biotechnologyforbiofuels.biomedcentral.com/articles/
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http://dx.doi.org/10.1186/s13068-019-1382-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6394018PMC
February 2019
3 Reads

Creation of a functional hyperthermostable designer cellulosome.

Biotechnol Biofuels 2019 28;12:44. Epub 2019 Feb 28.

1Department of Biomolecular Sciences, The Weizmann Institute of Science, 7610001 Rehovot, Israel.

Background: Renewable energy has become a field of high interest over the past decade, and production of biofuels from cellulosic substrates has a particularly high potential as an alternative source of energy. Industrial deconstruction of biomass, however, is an onerous, exothermic process, the cost of which could be decreased significantly by use of hyperthermophilic enzymes. An efficient way of breaking down cellulosic substrates can also be achieved by highly efficient enzymatic complexes called cellulosomes. Read More

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http://dx.doi.org/10.1186/s13068-019-1386-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6394049PMC
February 2019

Electron balancing under different sink conditions reveals positive effects on photon efficiency and metabolic activity of sp. PCC 6803.

Biotechnol Biofuels 2019 27;12:43. Epub 2019 Feb 27.

1Department of Solar Materials, Helmholtz Center for Environmental Research GmbH-UFZ, Permoserstraße 15, 04318 Leipzig, Germany.

Background: Cyanobacteria are ideal model organisms to exploit photosynthetically derived electrons or fixed carbon for the biotechnological synthesis of high value compounds and energy carriers. Much effort is spent on the rational design of heterologous pathways to produce value-added chemicals. Much less focus is drawn on the basic physiological responses and potentials of phototrophs to deal with natural or artificial electron and carbon sinks. Read More

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http://dx.doi.org/10.1186/s13068-019-1378-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6391784PMC
February 2019

Polysaccharide monooxygenase-catalyzed oxidation of cellulose to glucuronic acid-containing cello-oligosaccharides.

Biotechnol Biofuels 2019 27;12:42. Epub 2019 Feb 27.

Department of Mycology, Shandong Agricultural University, Taian, 271018 Shandong China.

Background: Polysaccharide monooxygenases (PMOs) play an important role in the enzymatic degradation of cellulose. They have been demonstrated to able to C6-oxidize cellulose to produce C6-hexodialdoses. However, the biological function of C6 oxidation of PMOs remains unknown. Read More

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http://dx.doi.org/10.1186/s13068-019-1384-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6391835PMC
February 2019

Engineering to produce the biogasoline isopentenol from plant biomass hydrolysates.

Biotechnol Biofuels 2019 27;12:41. Epub 2019 Feb 27.

3Joint BioEnergy Institute, Emeryville, CA USA.

Background: Many microbes used for the rapid discovery and development of metabolic pathways have sensitivities to final products and process reagents. Isopentenol (3-methyl-3-buten-1-ol), a biogasoline candidate, has an established heterologous gene pathway but is toxic to several microbial hosts. Reagents used in the pretreatment of plant biomass, such as ionic liquids, also inhibit growth of many host strains. Read More

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http://dx.doi.org/10.1186/s13068-019-1381-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6391826PMC
February 2019

Evaluation of divergent yeast genera for fermentation-associated stresses and identification of a robust sugarcane distillery waste isolate NGY10 for lignocellulosic ethanol production in SHF and SSF.

Biotechnol Biofuels 2019 27;12:40. Epub 2019 Feb 27.

Yeast Biofuel Group, DBT-ICGEB Center for Advanced Bioenergy Research, International Center for Genetic Engineering and Biotechnology (ICGEB), New Delhi, 110067 India.

Background: Lignocellulosic hydrolysates contain a mixture of hexose (C6)/pentose (C5) sugars and pretreatment-generated inhibitors (furans, weak acids and phenolics). Therefore, robust yeast isolates with characteristics of C6/C5 fermentation and tolerance to pretreatment-derived inhibitors are pre-requisite for efficient lignocellulosic material based biorefineries. Moreover, use of thermotolerant yeast isolates will further reduce cooling cost, contamination during fermentation, and required for developing simultaneous saccharification and fermentation (SSF), simultaneous saccharification and co-fermentation (SScF), and consolidated bio-processing (CBP) strategies. Read More

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http://dx.doi.org/10.1186/s13068-019-1379-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6391804PMC
February 2019
6.044 Impact Factor

Light/dark cycling causes delayed lipid accumulation and increased photoperiod-based biomass yield by altering metabolic flux in oleaginous sp.

Biotechnol Biofuels 2019 21;12:39. Epub 2019 Feb 21.

1Engineering Biology Research Center, Kobe University, 1-1 Rokkodai, Nada-ku, Kobe, 657-8501 Japan.

Background: Light/dark cycling is an inevitable outdoor culture condition for microalgal biofuel production; however, the influence of this cycling on cellular lipid production has not been clearly established. The general aim of this study was to determine the influence of light/dark cycling on microalgal biomass production and lipid accumulation. To achieve this goal, specific causative mechanisms were investigated using a metabolomics approach. Read More

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http://dx.doi.org/10.1186/s13068-019-1380-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6383270PMC
February 2019

Enhancing co-translational folding of heterologous protein by deleting non-essential ribosomal proteins in .

Biotechnol Biofuels 2019 21;12:38. Epub 2019 Feb 21.

1Guangdong Key Laboratory of Fermentation and Enzyme Engineering, School of Biology and Biological Engineering, South China University of Technology, Guangzhou, 510006 China.

Background: Translational regulation played an important role in the correct folding of heterologous proteins to form bioactive conformations during biogenesis. Translational pausing coordinates protein translation and co-translational folding. Decelerating translation elongation speed has been shown to improve the soluble protein yield when expressing heterologous proteins in industrial expression hosts. Read More

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http://dx.doi.org/10.1186/s13068-019-1377-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6383220PMC
February 2019
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Two-stage steam explosion pretreatment of softwood with 2-naphthol as carbocation scavenger.

Biotechnol Biofuels 2019 21;12:37. Epub 2019 Feb 21.

2School of Agricultural, Forest and Food Science, Bern University of Applied Science, Länggasse 85, 3052 Zollikofen, Switzerland.

Background:  Lignocellulosic biomass is considered as a potential source for sustainable biofuels. In the conversion process, a pretreatment step is necessary in order to overcome the biomass recalcitrance and allow for sufficient fermentable sugar yields in enzymatic hydrolysis. Steam explosion is a well known pretreatment method working without additional chemicals and allowing for efficient particle size reduction. Read More

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http://dx.doi.org/10.1186/s13068-019-1373-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6383249PMC
February 2019

,-dimethylformamide induces cellulase production in the filamentous fungus .

Biotechnol Biofuels 2019 19;12:36. Epub 2019 Feb 19.

State Key Lab of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, P.O.B. 311, Shanghai, 200237 China.

Background: The filamentous fungus produces cellulase enzymes that are widely studied for lignocellulose bioconversion to biofuel. ,-dimethylformamide (DMF) is a versatile organic solvent used in large quantities in industries.

Results: In this study, we serendipitously found that biologically relevant concentrations of extracellular DMF-induced cellulase production in the hyper-cellulolytic mutant Rut-C30 and wild-type strain QM6a. Read More

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http://dx.doi.org/10.1186/s13068-019-1375-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6380019PMC
February 2019
6.044 Impact Factor

Construction of consolidated bio-saccharification biocatalyst and process optimization for highly efficient lignocellulose solubilization.

Biotechnol Biofuels 2019 18;12:35. Epub 2019 Feb 18.

1CAS Key Laboratory of Biofuels, Shandong Provincial Key Laboratory of Synthetic Biology, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, China.

Background: The industrial conversion of biomass to high-value biofuels and biochemical is mainly restricted by lignocellulose solubilization. Consolidated bio-saccharification (CBS) is considered a promising process for lignocellulose solubilization depending on whole-cell biocatalysts that simultaneously perform effective cellulase production and hydrolysis. However, it usually takes a long time to reach a high saccharification level using the current CBS biocatalyst and process. Read More

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http://dx.doi.org/10.1186/s13068-019-1374-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6378752PMC
February 2019

Development of modified HCH-1 kinetic model for long-term enzymatic cellulose hydrolysis and comparison with literature models.

Biotechnol Biofuels 2019 18;12:34. Epub 2019 Feb 18.

1Department of Chemical Engineering, Texas A&M University, College Station, TX 77843-3122 USA.

Background: Enzymatic hydrolysis is a major step for cellulosic ethanol production. A thorough understanding of enzymatic hydrolysis is necessary to help design optimal conditions and economical systems. The original HCH-1 (Holtzapple-Caram-Humphrey-1) model is a generalized mechanistic model for enzymatic cellulose hydrolysis, but was previously applied only to the initial rates. Read More

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https://biotechnologyforbiofuels.biomedcentral.com/articles/
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http://dx.doi.org/10.1186/s13068-019-1371-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6378734PMC
February 2019
4 Reads

Microfluidic techniques for enhancing biofuel and biorefinery industry based on microalgae.

Biotechnol Biofuels 2019 15;12:33. Epub 2019 Feb 15.

2Department of Engineering and System Science, National Tsing Hua University, Hsinchu, Taiwan.

This review presents a critical assessment of emerging microfluidic technologies for the application on biological productions of biofuels and other chemicals from microalgae. Comparisons of cell culture designs for the screening of microalgae strains and growth conditions are provided with three categories: mechanical traps, droplets, or microchambers. Emerging technologies for the in situ characterization of microalgae features and metabolites are also presented and evaluated. Read More

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http://dx.doi.org/10.1186/s13068-019-1369-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6376642PMC
February 2019

Recent advances in lignin valorization with bacterial cultures: microorganisms, metabolic pathways, and bio-products.

Biotechnol Biofuels 2019 15;12:32. Epub 2019 Feb 15.

1School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing, 210094 China.

Lignin is the most abundant aromatic substrate on Earth and its valorization technologies are still under developed. Depolymerization and fragmentation are the predominant preparatory strategies for valorization of lignin to chemicals and fuels. However, due to the structural heterogeneity of lignin, depolymerization and fragmentation typically result in diverse product species, which require extensive separation and purification procedures to obtain target products. Read More

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http://dx.doi.org/10.1186/s13068-019-1376-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6376720PMC
February 2019