224 results match your criteria Biotechnology biotechnological equipment[Journal]


Increasing plasmid-based DNA vaccine construct (16 kb pSVK-HBVA) production in XL10-Gold through optimization of media component.

Biotechnol Biotechnol Equip 2015 Jan 22;29(1):164-174. Epub 2015 Jan 22.

Institute of Basic Medical Science, Academy of Military Medical Sciences , 27 Tai Ping Road, Beijing 100850 , China.

At present, there are production processes to produce protein by () fermentation. Research on the design and optimization of the plasmid fermentation medium, however, is less advanced. The fermentation medium that is optimized for plasmid DNA production is different from the medium that is optimized for protein production. Read More

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http://dx.doi.org/10.1080/13102818.2014.989103DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4697194PMC
January 2015
15 Reads

Subclinical hypothyroidism: association with cardiovascular risk factors and components of metabolic syndrome.

Biotechnol Biotechnol Equip 2015 Jan 16;29(1):157-163. Epub 2014 Dec 16.

Department of Microbiology-Virusology, Institute for Public Health Nis, University of Nis , Nis , Serbia.

The aim of this cross-sectional study was to evaluate the cardiovascular risk in patients with subclinical hypothyroidism (SH) and metabolic syndrome (MetS) components. The study included 60 patients with SH and a control group of 60 healthy volunteers, gender and age matched, with normal thyroid-stimulating hormone (TSH) and free thyroxin (FT4) concentration. The following measurements were made in all participants: TSH, FT4, thyroid peroxidase antibodies, anti-thyroglobulin antibodies, body mass index (BMI), waist circumference, blood pressure, fasting plasma glucose, total cholesterol (TC), low-density lipoprotein (LDL) cholesterol, high-density lipoprotein (HDL) cholesterol, triglycerides (TG), TC/HDL cholesterol and LDL/HDL cholesterol ratio, basal insulin level and homeostatic model assessment insulin resistance (HOMA-IR) index. Read More

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http://dx.doi.org/10.1080/13102818.2014.991136DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4684069PMC
January 2015
3 Reads

Complementary annealing mediated by exonuclease: a method for seamless cloning and conditioning site-directed mutagenesis.

Biotechnol Biotechnol Equip 2015 Jan 10;29(1):105-110. Epub 2014 Dec 10.

Institute of Developmental and Regenerative Biology, College of Life and Environmental Sciences, Hangzhou Normal University , #16 Xuelin Street, Hangzhou , Zhejiang 310036 , China.

Traditional cut-paste DNA cloning is often limited by the availability of restriction enzyme sites. Here, we described the complementary annealing mediated by exonuclease (CAME), in which the insert or vector fragment is amplified to carry sequences complementary to the other, and both fragments are modified by exonuleases to create directional single-stranded overhangs. The two recessed DNA fragments are joined through complementary strand annealing. Read More

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http://dx.doi.org/10.1080/13102818.2014.988094DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4686905PMC
January 2015
2 Reads

Optimization of lipase production on agro-industrial residue medium by (NRLL B-2641) using response surface methodology.

Biotechnol Biotechnol Equip 2015 Jan 16;29(1):64-71. Epub 2014 Dec 16.

The Technology of Chemistry, Vocational School, Tunceli University , Tunceli , Turkey.

The aim of our research was to explore the most cost-efficient and optimal medium composition for the production of lipase from (NRLL B-2641) culture grown on sunflower oil cake (SuOC) by applying response surface methodology (RSM). The oil cake was used instead of carbon sources. Peptone, ammonium sulphate and the carbon source (SuOC) were the most important factors as it is obligatory for microbial growth. Read More

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http://dx.doi.org/10.1080/13102818.2014.991635DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4684071PMC
January 2015

LAU 13: a new source of keratinase and its multi-functional biocatalytic applications.

Biotechnol Biotechnol Equip 2015 Jan 1;29(1):54-63. Epub 2014 Dec 1.

Department of Microbiology, School of Life Science, University of KwaZulu-Natal , Pietermaritzburg , South Africa.

A newly isolated bacterium identified as based on biochemical tests and 16S rRNA analysis and its mutant variant created by exposure to ultraviolet radiation at 254 nm were investigated for keratinolytic activity. The wild-type strain produced 35.4-50. Read More

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http://dx.doi.org/10.1080/13102818.2014.986360DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4684068PMC
January 2015
1 Read

Degradation kinetics and pathway of phenol by and species.

Biotechnol Biotechnol Equip 2015 Jan 7;29(1):45-53. Epub 2015 Jan 7.

Institute of Environmental Studies, University of Karachi , Karachi -75270, Pakistan.

This article elucidates that strain (IES--1) is a versatile toxic organic compound degrader. With the degradation of malathion and cypermethrin (studied by other researchers previously), this strain was able to degrade phenol. Two other indigenous soil flora (i. Read More

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http://dx.doi.org/10.1080/13102818.2014.991638DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4684072PMC
January 2015
4 Reads

Effect of cyprodinil and fludioxonil pesticides on bovine liver catalase activity.

Biotechnol Biotechnol Equip 2015 Jan 7;29(1):40-44. Epub 2015 Jan 7.

Department of Chemistry, Faculty of Science and Letters, University of Adiyaman , Adiyaman , Turkey.

This study investigated if the use of the pesticides cyprodinil and fludioxonil produced an inhibitory effect on the bovine liver catalase (CAT) activity. It was documented that the activity of the enzyme decreased with increasing concentrations of cyprodinil and fludioxonil from 0 to 500 ppm. At pesticide concentrations of 250 and 500 ppm, the activity of CAT remained unchanged and passed to a steady state. Read More

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http://dx.doi.org/10.1080/13102818.2014.992740DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4684073PMC
January 2015
1 Read

Probiotic and cultural characteristic of strain 4/13 of human origin.

Biotechnol Biotechnol Equip 2014 Nov 31;28(6):1084-1088. Epub 2014 Oct 31.

R & D Center, LB-Bulgaricum PLC , Sofia , Bulgaria.

within the group is a major species in the human microflora. The potential probiotic properties of a strain of human origin were evaluated. Out of 17 studied strains, 4/13 showed the highest immunomodulatory effect (induction of interferon gamma measured by sandwich enzyme-linked immunosorbent assay) in Balb/c mouse splenocytes and the highest rate of adhesion to Caco-2 human epithelial cells. Read More

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http://dx.doi.org/10.1080/13102818.2014.974303DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4648341PMC
November 2014
1 Read

Research into acetone removal from air by biofiltration using a biofilter with straight structure plates.

Biotechnol Biotechnol Equip 2015 Mar 3;29(2):404-413. Epub 2015 Feb 3.

Faculty of Environmental Engineering, Research Institute of Environment Protection, Vilnius Gediminas Technical University , Vilnius , Lithuania.

The biological air treatment method is based on the biological destruction of organic compounds using certain cultures of microorganisms. This method is simple and may be applied in many branches of industry. The main element of biological air treatment devices is a filter charge. Read More

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http://dx.doi.org/10.1080/13102818.2015.1006413DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4434071PMC
March 2015
3 Reads

De novo transcriptome analysis of a medicinal fungi and identification of SSR markers.

Biotechnol Biotechnol Equip 2015 Mar 5;29(2):395-403. Epub 2015 Feb 5.

Department of Forest Bioengineering, College of Life Science, Northeast Forestry University , Harbin , P.R. China.

The aim of this study was to facilitate gene discovery for functional genome studies and to identify simple sequence repeat (SSR) markers for molecular-assisted selection in . The transcriptome of was sequenced using а high-throughput RNA sequencing system - the Illumina Hiseq 2000. A total of 16,383,818 clean sequencing reads, 35,532 contigs and 25,811 unigenes were postulated. Read More

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http://dx.doi.org/10.1080/13102818.2015.1008228DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4434122PMC
March 2015
7 Reads

Effect of hydrophilic polymers on the wettability, static and dynamic, of solid substrate covered by confluent monolayer of air-damaged SIRC cells.

Biotechnol Biotechnol Equip 2015 Mar 13;29(2):390-394. Epub 2015 Jan 13.

Department of Biochemistry, Faculty of Biology, Sofia University 'St. Kliment Ohridski' , Sofia , Bulgaria.

The aim of this study was to evaluate the possible implementation of hydrophilic polymers as recovery agents in air-damaged corneal cells. The sessile bubble technique was implemented to measure the wetting properties of four selected polymers: hydroxyethyl cellulose (HEC), sodium chondroitin sulphate (SCS), hydroxypropyl-methylcellulose (HPMC) and poloxamer F127 (PO12), at equilibrium conditions and in the case of advancing and receding contact angle. For testing the wetting properties of the polymers, glass slides covered with a confluent monolayer of Statens Seruminstitut rabbit cornea (SIRC) cells were used. Read More

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http://dx.doi.org/10.1080/13102818.2014.997541DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4433808PMC
March 2015
1 Read

The positive effects of Mn on nitrogen use and surfactin production by ATCC 21332.

Biotechnol Biotechnol Equip 2015 Mar 12;29(2):381-389. Epub 2015 Feb 12.

College of Environmental Science and Engineering, State Key Laboratory of Pollution Control and Resource Reuse, Key Laboratory of Yangtze River Water Environment of the Ministry of Education, the Collaborative Innovation Center of Advanced Technology and Equipment for Water Pollution Control, the Collaborative Innovation Center for Regional Environmental Quality, Tongji University , Shanghai , China.

Surfactin, one of the most effective biosurfactants, has great potential in commercial applications. Studies on effective methods to reduce surfactin's production cost are always a hotspot in the research field of biosurfactants. The aim of this study was to reveal the role of Mn in promoting the biosynthesis of surfactin by ATCC 21332, which could arise more targeted suggestions on surfactin yield promotion. Read More

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http://dx.doi.org/10.1080/13102818.2015.1006905DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4433937PMC
March 2015
4 Reads

Optimization of carbon source and glucose feeding strategy for improvement of L-isoleucine production by .

Biotechnol Biotechnol Equip 2015 Mar 4;29(2):374-380. Epub 2015 Feb 4.

National and Local United Engineering Lab of Metabolic Control Fermentation Technology, Tianjin University of Science and Technology , Tianjin , China ; Key Laboratory of Industrial Microbiology of Education Ministry, Tianjin University of Science and Technology , Tianjin , China.

Fed-batch cultivations of L-isoleucine-producing TRFP (SG, -ABA, with a pTHR101 plasmid containing a thr operon and ilvA) were carried out on different carbon sources: glucose, sucrose, fructose, maltose and glycerol. The results indicated that sucrose was the best initial carbon source for L-isoleucine production and then sucrose concentration of 30 g·L was determined in the production medium. The results of different carbon sources feeding showed that the glucose solution was the most suitable feeding media. Read More

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http://dx.doi.org/10.1080/13102818.2015.1006899DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4433842PMC
March 2015
24 Reads

Evaluation of dentinal defect formation after root canal preparation with two reciprocating systems and hand instruments: an study.

Biotechnol Biotechnol Equip 2015 Mar 13;29(2):368-373. Epub 2015 Jan 13.

Department of Endodontics, Faculty of Dentistry, Istanbul University , Istanbul , Turkey.

The purpose of this study was to evaluate the presence of dentinal defects after root canal preparation with hand instruments and two different reciprocating instruments. Sixty freshly extracted mandibular incisor teeth were selected for this study. On the basis of root length, mesiodistal and buccolingual dimensions, the teeth were allocated into three identical experimental groups ( = 15) and one control group ( = 15). Read More

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http://dx.doi.org/10.1080/13102818.2014.996982DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4434093PMC
March 2015
4 Reads

Resin cementation of zirconia ceramics with different bonding agents.

Biotechnol Biotechnol Equip 2015 Mar 9;29(2):363-367. Epub 2015 Jan 9.

Department of Prosthetic Dentistry, Faculty of Dentistry, Gazi University , Ankara , Turkey.

The aim of this study was to evaluate the effects of sandblasting and different chemical bonding agents on shear bond strength of zirconia and conventional resin cement. In this study, 35 zirconia specimens were treated as follows: Group I: control; Group II: sandblasting; Group III: sandblasting + Monobond S; Group IV: sandblasting + Monobond Plus; Group V: sandblasting + Z-Prime Plus. The specimens in each group were bonded with conventional composite resin cement Variolink II. Read More

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http://dx.doi.org/10.1080/13102818.2014.996606DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4433915PMC
March 2015
6 Reads

Distribution patterns of carbohydrates in murine glycocalyx.

Biotechnol Biotechnol Equip 2015 Mar 29;29(2):357-362. Epub 2015 Jan 29.

Department of Experimental Morphology, Institute of Experimental Morphology, Pathology and Anthropology with Museum, Bulgarian Academy of Sciences , Sofia , Bulgaria.

Enterocytes are unique cells governing an array of processes. They are covered by the gut glycocalyx, which is an extraneous carbohydrate-rich coat and an integral part of the plasma membrane. The intestinal glycocalyx and secreted mucins constitute a glycosylated milieu which has a number of physiological and protective functions. Read More

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http://dx.doi.org/10.1080/13102818.2014.999214DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4434138PMC
March 2015
1 Read

Frequency of the common promoter polymorphism in a population from central Bulgaria.

Biotechnol Biotechnol Equip 2015 Mar 28;29(2):351-356. Epub 2015 Jan 28.

Department of Chemistry and Biochemistry, Medical Faculty , Trakia University , Stara Zagora , Bulgaria.

Matrix metalloproteinases (MMPs) are a family of highly homologous extracellular Zn-dependent endopeptidases, also known as matrixins. MMP-2 (gelatinase A) and MMP-9 (gelatinase B) are considered to play a key role in a variety of physiological processes as well as in the development and progression of a vast majority of pathological conditions. Most of the genes encoding MMPs, including MMP-2, are highly polymorphic. Read More

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http://dx.doi.org/10.1080/13102818.2014.995411DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4433921PMC
March 2015
4 Reads

Severe pre-eclampsia complicated by HELLP syndrome alterations in the structure of the umbilical cord (morphometric and immunohistochemical study).

Biotechnol Biotechnol Equip 2015 Mar 19;29(2):345-350. Epub 2015 Jan 19.

Department of Obstetrics and Gynecology, Diyarbakır Education Research Hospital , Diyarbakır , Turkey.

The aim of this study was to evaluate the morphometric and immunohistochemistry in umbilical cords from patients with severe pre-eclampsia with and without haemolysis, elevated liver enzymes and low platelets (HELLP) syndrome. The patient and control groups were similar according to baseline obstetric characteristics. White blood cell count in patients with HELLP syndrome and the control group was significantly increased among patients with HELLP syndrome ( < 0. Read More

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http://dx.doi.org/10.1080/13102818.2014.991545DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4433885PMC
March 2015
8 Reads

Effect of BMP-6 on development and maturation of mouse preantral follicles .

Biotechnol Biotechnol Equip 2015 Mar 14;29(2):336-344. Epub 2015 Jan 14.

Department of Histology and Embryology, Medical College, Nantong University , Nantong , P.R. China.

The aim of this study was to investigate the effect and mechanism of bone morphogenetic protein-6 (BMP-6) on the growth and maturation of mouse follicles . Preantral follicles isolated from mice were incubated with recombinant human BMP-6 (rhBMP-6) before analysis. BMP-6 expression was detected by immunofluorescence and western blot. Read More

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http://www.tandfonline.com/doi/abs/10.1080/13102818.2014.996
Publisher Site
http://dx.doi.org/10.1080/13102818.2014.996605DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4434052PMC
March 2015
5 Reads

Relationship between vitamin D status and immunosuppressive therapy in kidney transplant recipients.

Biotechnol Biotechnol Equip 2015 Mar 8;29(2):331-335. Epub 2015 Jan 8.

Laboratory of Therapeutic Drug Management & Clinical Pharmacology, University Hospital 'Alexandrovska', Medical University of Sofia , Sofia , Bulgaria.

There is a growing body of evidence for the protective role of vitamin D in diabetes mellitus (DM), infection, cancer, cardiovascular disease, immune disorders and kidney function. Considering the reported high prevalence of vitamin D insufficiency among kidney transplant recipients (KTRs), the aim of this study was to assess the influence of immunosuppressive therapy and other factors on vitamin D status in such patients. The study included 289 KTRs (189 males and 100 females) who consented to participate. Read More

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http://dx.doi.org/10.1080/13102818.2014.995415DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4433925PMC

Toxigenic profiles and trinucleotide repeat diversity of species isolated from banana fruits.

Biotechnol Biotechnol Equip 2015 Mar 8;29(2):324-330. Epub 2015 Jan 8.

Botany and Microbiology Department, Faculty of Science, King Saud University , Riyadh , Saudi Arabia.

Infesting species isolated from banana fruit samples were identified and quantified by morphological, mycotoxicological and molecular tools. A total of 19 isolates were obtained: was most predominant (26%), followed by (16%), (16%), (10.5%), (10. Read More

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http://dx.doi.org/10.1080/13102818.2014.995519DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4433895PMC

Optimization and purification of mannanase produced by an alkaliphilic-thermotolerant N1 isolated from Bani Salama Lake in Wadi El-Natron.

Biotechnol Biotechnol Equip 2015 Mar 13;29(2):315-323. Epub 2015 Jan 13.

Botany and Microbiology Department, Faculty of Science, Alexandria University , Alexandria , Egypt.

An alkaliphilic-thermotolerant N1 isolated from Bani Salama Lake, Wadi El-Natron, Egypt, was proved to produce mannanase enzyme. Optimization of the fermentation medium components using Plackett-Burman design was applied. Glucose and inoculum size were found to be the most significant factors enhancing the production of the enzyme. Read More

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http://dx.doi.org/10.1080/13102818.2014.995932DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4433781PMC
March 2015
9 Reads

Assessment of genetic characteristics of germplasms in Xinjiang Province (China) by RAPD and ISSR markers.

Biotechnol Biotechnol Equip 2015 Mar 29;29(2):309-314. Epub 2015 Jan 29.

Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Xinjiang Medical University , Urumqi , Xinjiang , PR China.

is a medicinal treasure trove that grows extensively on fertile pastures in Xinjiang Province (China); however, its molecular genetic characteristics are still poorly studied. We studied Reichb., Stapf. Read More

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http://dx.doi.org/10.1080/13102818.2015.1004899DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4434092PMC
March 2015
1 Read

Light-emitting diodes and their potential in callus growth, plantlet development and saponin accumulation during somatic embryogenesis of Ha Grushv.

Biotechnol Biotechnol Equip 2015 Mar 14;29(2):299-308. Epub 2015 Jan 14.

Research Center of Ginseng and Medicinal Materials, National Institute of Medicinal Materials , Vietnam.

In recent years, LED (light-emitting diode) has been the subject of research within the field of plant growth and development. However, there has been little discussion about using LED cultures of , one of the important medicinal plants belonging to the genus. This study examines the influence of various LED lamps on callus growth and plant formation of . Read More

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http://dx.doi.org/10.1080/13102818.2014.1000210DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4433904PMC
March 2015
9 Reads

The effects of composite photosynthetic bacterial inoculant PS21 on the biochemical characteristics of wheat seedlings under tetrabromobisphenol A stress.

Biotechnol Biotechnol Equip 2015 Mar 13;29(2):289-298. Epub 2015 Jan 13.

College of Life Science and Agronomy, Zhoukou Normal University , Zhoukou 466001 , China.

The aim of this study was to investigate whether the composite photosynthetic bacterial inoculant PS21 alleviate the damage inflicted on wheat seedlings by tetrabromobisphenol A (TBBPA). The biochemical characteristics of wheat seedlings were analysed through laboratory simulation after co-treating seedlings with PS21 and 0, 5, 10, 20, 40, 80 and 100 mg kg TBBPA, respectively. The results showed that TBBPA reduced the total chlorophyll content and increased the malondialdehyde (MDA) content. Read More

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http://dx.doi.org/10.1080/13102818.2014.999298DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4433861PMC

Screening for antibacterial activity of some Turkish plants against fish pathogens: a possible alternative in the treatment of bacterial infections.

Biotechnol Biotechnol Equip 2015 Mar 17;29(2):281-288. Epub 2015 Feb 17.

Field Crops Department, Agricultural and Natural Sciences Faculty, Abant Izzet Baysal University , Bolu , Turkey.

The antibacterial activity of ethanolic and aqueous crude extracts from 36 plants in Turkey, including seven endemic species, against fish pathogens was studied using the disc diffusion assay. The extract that was most active against all microbial strains, except , was that of . Some of the extracts also showed a very broad spectrum of potent antimicrobial activity. Read More

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http://dx.doi.org/10.1080/13102818.2015.1006445DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4433805PMC
March 2015
2 Reads

Antibiotic resistance of Gram-negative benthic bacteria isolated from the sediments of Kardzhali Dam (Bulgaria).

Biotechnol Biotechnol Equip 2015 Mar 20;29(2):274-280. Epub 2015 Jan 20.

Department of Biochemistry and Microbiology, Faculty of Biology, University of Plovdiv "Paisii Hilendarski" , Plovdiv , Bulgaria.

The aim of the present study was to carry out a preliminary assessment for the occurrence of bacterial strains resistant to frequently used antibiotics in the sediments beneath the sturgeon cage farm in Kardzhali Dam (Bulgaria). Samples were taken from the top 2 cm of sediments under a fish farm and from a control station in the aquatory of the reservoir in the period July-October 2011. Surveillance of bacterial susceptibility to 16 antimicrobial agents was performed for 160 Gram-negative strains ( - 100 strains; - 30 strains; and - 30 strains). Read More

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http://dx.doi.org/10.1080/13102818.2014.998160DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4433961PMC

Geneticin (G418) resistance and electroporation-mediated transformation of and .

Biotechnol Biotechnol Equip 2015 Mar 23;29(2):268-273. Epub 2015 Jan 23.

Department of Molecular Biology and Genetics, Faculty of Science, Istanbul University , 34134 Vezneciler , Istanbul , Turkey.

and are phytopathogenic species causing scab and root rot diseases in all small grain cereals worldwide including Turkey. In this study, resistance levels to geneticin (G418) of 14 and 24 isolates collected from cereals were determined. Fungal cultures were grown on potato dextrose agar medium supplemented with 0, 25, 50, 75 and 100 µg/mL of G418. Read More

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http://dx.doi.org/10.1080/13102818.2014.996978DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4434094PMC
March 2015
5 Reads

Frequent problems and their resolutions by using thermal asymmetric interlaced PCR (TAIL-PCR) to clone genes in T-DNA tagged mutants.

Biotechnol Biotechnol Equip 2015 Mar 14;29(2):260-267. Epub 2015 Jan 14.

Department of MOE Key Laboratory of Cell Activities and Stress Adaptations, School of Life Sciences, Lanzhou University, Lanzhou , Gansu , P.R. China.

T-DNA insertional mutagenesis is a powerful tool in functional genomics research. Previous studies have developed thermal asymmetric interlaced polymerase chain reaction (TAIL-PCR) as an efficient strategy in isolation of DNA sequences adjacent to known sequences in T-DNA tagged mutants. However, a number of problems are encountered when attempts are made to clone flanking sequences in T-DNA tagged mutants. Read More

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http://dx.doi.org/10.1080/13102818.2014.998161DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4433792PMC
March 2015
7 Reads

Molecular cloning, sequence characterization and expression pattern of Rab18 gene from watermelon ().

Authors:
Xiao Xinli Peng Lei

Biotechnol Biotechnol Equip 2015 Mar 3;29(2):255-259. Epub 2015 Feb 3.

Department of Horticulture, College of Horticulture and Landscape, Yunnan Agricultural University , Kunming 650201 , China.

The complete mRNA sequence of watermelon Rab18 gene was amplified through the rapid amplification of cDNA ends (RACE) method. The full-length mRNA was 1010 bp containing a 645 bp open reading frame, which encodes a protein of 214 amino acids. Sequence analysis revealed that watermelon Rab18 protein shares high homology with the Rab18 of cucumber (99%), muskmelon (98%), (90%), tomato (89%), wine grape (89%) and potato (88%). Read More

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http://dx.doi.org/10.1080/13102818.2015.1008198DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4434096PMC
March 2015
11 Reads

Marker-assisted backcrossing: a useful method for rice improvement.

Biotechnol Biotechnol Equip 2015 Mar 26;29(2):237-254. Epub 2015 Feb 26.

Department of Plant Pathology, Bangladesh Rice Research Institute , Gazipur , Bangladesh.

The world's population is increasing very rapidly, reducing the cultivable land of rice, decreasing table water, emerging new diseases and pests, and the climate changes are major issues that must be addressed to researchers to develop sustainable crop varieties with resistance to biotic and abiotic stresses. However, recent scientific discoveries and advances particularly in genetics, genomics and crop physiology have opened up new opportunities to reduce the impact of these stresses which would have been difficult if not impossible as recently as the turn of the century. Marker assisted backcrossing (MABC) is one of the most promising approaches is the use of molecular markers to identify and select genes controlling resistance to those factors. Read More

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http://dx.doi.org/10.1080/13102818.2014.995920DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4433898PMC
March 2015
62 Reads
9 Citations
0.380 Impact Factor

Myconanoparticles: synthesis and their role in phytopathogens management.

Biotechnol Biotechnol Equip 2015 Mar 9;29(2):221-236. Epub 2015 Mar 9.

Plant Pathology Research Institute, Agricultural Research Center (ARC) , Giza , Egypt ; Unit of Excellence in Nano-Molecular Plant Pathology Research Institute , Giza , Egypt.

Nanotechnology can offer green and eco-friendly alternatives for plant disease management. Apart from being eco-friendly, fungi are used as bio-manufacturing units, which will provide an added benefit in being easy to use, as compared to other microbes. The non-pathogenic nature of some fungal species in combination with the simplicity of production and handling will improve the mass production of silver nanoparticles. Read More

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http://dx.doi.org/10.1080/13102818.2015.1008194DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4433920PMC
March 2015
3 Reads

An overview of technologies for immobilization of enzymes and surface analysis techniques for immobilized enzymes.

Biotechnol Biotechnol Equip 2015 Mar 17;29(2):205-220. Epub 2015 Feb 17.

Department of Chemistry, Faculty of Science, Universiti Teknologi Malaysia , Skudai 81310 , Johor , Malaysia.

The current demands of sustainable green methodologies have increased the use of enzymatic technology in industrial processes. Employment of enzyme as biocatalysts offers the benefits of mild reaction conditions, biodegradability and catalytic efficiency. The harsh conditions of industrial processes, however, increase propensity of enzyme destabilization, shortening their industrial lifespan. Read More

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http://www.tandfonline.com/doi/abs/10.1080/13102818.2015.100
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http://dx.doi.org/10.1080/13102818.2015.1008192DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4434042PMC
March 2015
24 Reads
0.379 Impact Factor

Assessment of food habits in children aged 6-12 years and the risk of caries.

Biotechnol Biotechnol Equip 2015 Jan 12;29(1):200-204. Epub 2014 Dec 12.

Department of Pediatric Dentistry, Faculty of Dental Medicine, Medical University of Sofia , Sofia , Bulgaria.

Food is necessary for the proper growth and development of children. The excessive intake of low-molecular carbohydrates constitutes a serious health issue, which has an unfavourable impact on the dental health status. The aim of this study was to assess the food habits in healthy children aged 6-12 years and the effect on their oral risk profile. Read More

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http://dx.doi.org/10.1080/13102818.2014.989180DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4433830PMC
January 2015
1 Read

Time lag model for batch bioreactor simulation accounting the effect of micro-organism mortality.

Biotechnol Biotechnol Equip 2015 Jan 7;29(1):195-199. Epub 2015 Jan 7.

Faculty of Mathematics and Informatics, University of Plovdiv , Plovdiv , Bulgaria.

In the present work, a generalization of the classical model of Monod accounting the influence of both delayed and instant mortalities on the dynamics of the micro-organism population is proposed. The model was analysed and compared with respect to its quality and applicability for simulation of the cultivation process of micro-organisms. Existence of a unique global positive solution of the Cauchy problem for the proposed model is proved and explicit relations between the decay parameters and the nutrition substrate concentration are obtained. Read More

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http://dx.doi.org/10.1080/13102818.2014.993111DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4434070PMC
January 2015

Biological evaluation of synthesized allicin and its transformation products obtained by microwaves in methanol: antioxidant activity and effect on cell growth.

Biotechnol Biotechnol Equip 2015 Jan 8;29(1):189-194. Epub 2015 Jan 8.

Department of Chemistry and Chemical Technology, Faculty of Technology , University of Niš , Leskovac , Serbia.

Allicin is the most biologically active substance present in garlic. It can be synthesized or obtained by extraction of fresh garlic. Transformation products of allicin are also biologically active. Read More

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http://dx.doi.org/10.1080/13102818.2014.994267DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4433831PMC
January 2015
5 Reads
0.380 Impact Factor

Changes in the functional characteristics of tumor and normal cells after treatment with extracts of white dead-nettle.

Biotechnol Biotechnol Equip 2015 Jan 12;29(1):181-188. Epub 2014 Dec 12.

Department of Cytology, Histology and Embryology, Faculty of Biology, Sofia University 'St. Kl. Ohridski' , Sofia , Bulgaria.

L. is a perennial herb widely used in folk medicine. It possesses a wide spectrum of therapeutic activities (anti-inflammatory, astringent, antiseptic, antibiotic, antispasmodic, antioxidant and anti-proliferative). Read More

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http://dx.doi.org/10.1080/13102818.2014.989094DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4433899PMC
January 2015
62 Reads

Delivery of size-controlled long-circulating polymersomes in solid tumours, visualized by quantum dots and optical imaging .

Biotechnol Biotechnol Equip 2015 Jan 26;29(1):175-180. Epub 2014 Nov 26.

Diagnostic Imaging Program, Molecular Imaging Center, National Institute of Radiological Sciences, Anagawa , Chiba , Japan.

The present study was designed to investigate whether poly-ion complex hollow vesicles (polymersomes), based on chemically modified chitosan, are appropriate for passive tumour targeting in the context of their application as drug carriers. The experiments were performed on colon cancer-grafted mice. The mice were subjected to anaesthesia and injected intravenously with water-soluble nanoparticles: (1) QD-labelled polymersomes (average size ∼120 nm; size distribution ∼10%) or (2) native QD. Read More

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http://dx.doi.org/10.1080/13102818.2014.984894DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4434099PMC
January 2015
1 Read

Rapid identification of by pulsed-field gel electrophoresis.

Biotechnol Biotechnol Equip 2015 Jan 18;29(1):152-156. Epub 2014 Nov 18.

Department of Botany, Faculty of Science, Suez Canal University , Ismailia , Egypt.

Twenty clinical isolates recovered from patients admitted to The General Hospital in Ismailia Governorate (Egypt) were examined in this study. We analysed ATCC 9027 (as a control strain) and 19 of the isolates after digestion with restriction endonuclease. After this we conducted a pulsed-field gel electrophoresis (PFGE) and typed the obtained 10 unique patterns, designated as A, A1, B, B1, C, C1, D, D1, E and F. Read More

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http://dx.doi.org/10.1080/13102818.2014.981065DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4433924PMC
January 2015
1 Read

Cystathionine gamma-lyase of perivascular adipose tissue with reversed regulatory effect in diabetic rat artery.

Biotechnol Biotechnol Equip 2015 Jan 18;29(1):147-151. Epub 2014 Dec 18.

Department of Animal and Human Physiology, Faculty of Biology, Sofia University St. Kliment Ohridski , Sofia , Bulgaria.

The aim of this study is to reveal the regulatory role of cystathionine gamma-lyase (CSE), the main source of hydrogen sulphide (HS) in perivascular adipose tissue (PVAT), of diabetic rats. Diabetes was induced in male rats by a single intraperitoneal injection of streptozotocin. Animals with glucose levels above 20 mmol/L were determined as diabetic. Read More

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http://www.tandfonline.com/doi/abs/10.1080/13102818.2014.991
Publisher Site
http://dx.doi.org/10.1080/13102818.2014.991565DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4433913PMC
January 2015
7 Reads

Combination of Hook F and angiotensin receptor blocker synergistically reduces excretion of urinary podocytes in patients with type 2 diabetic kidney disease.

Biotechnol Biotechnol Equip 2015 Jan 11;29(1):139-146. Epub 2014 Dec 11.

Department of Nephrology, Affiliated Hospital of Medical College, Qingdao University , Qingdao , Shandong , P.R. China.

The aim of this study was to investigate whether Hook F (TwHF) and irbesartan could synergistically affect the urinary excretion of podocytes and proteins in type 2 diabetic kidney disease (DKD) patients and the underlying mechanisms. Forty DKD patients were divided into a DI group (DKD patients treated with irbesartan alone) and a DTI group (DKD patients treated with Tripterygium wilfordii Hook F and irbesartan). Urinary podocytes were observed by immunofluorescence. Read More

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http://dx.doi.org/10.1080/13102818.2014.989727DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4434037PMC
January 2015

A polysaccharide from prevents hepatic inflammatory response caused by carbon tetrachloride.

Biotechnol Biotechnol Equip 2015 Jan 6;29(1):132-138. Epub 2014 Dec 6.

Hefei University of Technology, School of Biotechnology and Food Engineering , Hefei , China.

is a precious herbal medicine in China, which exhibits a variety of restorative and therapeutic effects. This study aimed at investigating the hepatoprotective effects of a polysaccharide (DHP1A) isolated from via water extraction, diethylaminoethyl (DEAE) cellulose anion exchange and size exclusion chromatography. The animal experiment indicated that the oral administration of DHP1A obviously reduced the levels of alanine aminotransferase, aspartate aminotransferase, lactate dehydrogenase and 8-hydroxy-2'-deoxyguanosine in the serum of mice treated with carbon tetrachloride (CCl), suggesting the hepatoprotective potential of this polysaccharide. Read More

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http://dx.doi.org/10.1080/13102818.2014.987514DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4434038PMC
January 2015
6 Reads

Tetramethylpyrazine inhibits CTGF and Smad2/3 expression and proliferation of hepatic stellate cells.

Biotechnol Biotechnol Equip 2015 Jan 28;29(1):124-131. Epub 2015 Jan 28.

Department of Gastroenterology, The Second Affiliated Hospital of Chongqing Medical University , Chongqing 400010 , China.

To study the effects of tetramethylpyrazine (TMP) on the proliferation of hepatic stellate cells-T6 (HSC-T6), and the expression of connective tissue growth factor (CTGF) and Smad2/3 in these cells, HSC-T6 cells were cultured with TMP at different concentrations after transforming growth factor-β1 (TGF-β1) stimulation. MTT assay was used to assess the cell proliferation. Cells were divided into the control group, TGF-β1-treated group and TMP-treated groups, which were treated with different concentrations of TMP. Read More

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http://dx.doi.org/10.1080/13102818.2014.984382DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4433940PMC
January 2015
4 Reads

Correlation between vascular endothelial growth factor levels and prognosis of hepatocellular carcinoma patients receiving radiofrequency ablation.

Biotechnol Biotechnol Equip 2015 Jan 1;29(1):119-123. Epub 2014 Dec 1.

Xinjiang Chinese Medicine Hospital , Huanghe road , Wulumuqi , 830054 , P. R. China.

This study is aimed to investigate the effect of serum vascular endothelial growth factor (VEGF) levels on prognosis of hepatocellular carcinoma (HCC) patients receiving radiofrequency ablation (RFA). The 110 HCC patients who received computed tomography (CT) guided RFA were enrolled in this study. The levels of serum VEGF were determined before and after RFA by enzyme-linked immunosorbent assay (ELISA). Read More

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http://dx.doi.org/10.1080/13102818.2014.981776DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4434046PMC
January 2015
3 Reads

Whole genome microarray analysis in non-small cell lung cancer.

Biotechnol Biotechnol Equip 2015 Jan 19;29(1):111-118. Epub 2015 Jan 19.

Department of Medical Genetics, Faculty of Medicine, Medical University of Sofia , Sofia , Bulgaria.

Lung cancer is a serious health problem, since it is one of the leading causes for death worldwide. Molecular-cytogenetic studies could provide reliable data about genetic alterations which could be related to disease pathogenesis and be used for better prognosis and treatment strategies. We performed whole genome oligonucleotide microarray-based comparative genomic hybridization in 10 samples of non-small cell lung cancer. Read More

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http://dx.doi.org/10.1080/13102818.2014.989179DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4433918PMC
January 2015
34 Reads

Transepithelial resistance in human bestrophin-1 stably transfected Madin-Darby canine kidney cells.

Biotechnol Biotechnol Equip 2015 Jan 10;29(1):101-104. Epub 2014 Dec 10.

Faculty of Biology, Sofia University "St. Kliment Ohridski" , Sofia , Bulgaria.

Bestrophin-1 (Best1) is a transmembrane protein, found in the basolateral plasma membrane of retinal pigmented epithelial cells. The exact structure and functions of Best1 protein are still unclear. The protein is thought to be a regulator of ion channels, or an ion channel itself: it was shown to be permeable for chloride, thiocyanate, bicarbonate, glutamate and γ-aminobutyric acid (GABA). Read More

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http://www.tandfonline.com/doi/abs/10.1080/13102818.2014.988
Publisher Site
http://dx.doi.org/10.1080/13102818.2014.988078DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4434050PMC
January 2015
7 Reads

Nitroxidergic modulation of behavioural, cardiovascular and immune responses, and brain NADPH diaphorase activity upon morphine tolerance/dependence in rats.

Biotechnol Biotechnol Equip 2015 Jan 13;29(1):92-100. Epub 2014 Dec 13.

Department of Pharmacology and Toxicology, Medical Faculty, Medical University of Sofia , Sofia , Bulgaria.

Opioid and non-opioid effects of acute and chronic morphine administration on behaviour, cardiovascular responses, cell proliferation and apoptosis and nitric-oxide synthase (NOS) activity were studied in rats. A novel score-point scale was introduced to quantify the signs of opioid withdrawal syndrome. NOS inhibitor L-NAME (N-nitro-L-arginine methyl ester) was applied to reveal the role of NOS/NO pathway in the modulation of morphine-induced and responses. Read More

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http://www.tandfonline.com/doi/abs/10.1080/13102818.2014.990
Publisher Site
http://dx.doi.org/10.1080/13102818.2014.990924DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4434040PMC
January 2015
2 Reads

Antimicrobial activity and antibiotic susceptibility of and spp. intended for use as starter and probiotic cultures.

Biotechnol Biotechnol Equip 2015 Jan 11;29(1):84-91. Epub 2014 Dec 11.

Lactina Ltd. , Bankya , Bulgaria.

Antimicrobial activity and antibiotic susceptibility were tested for 23 and three strains isolated from different ecological niches. Agar-well diffusion method was used to test the antagonistic effect (against , , and ) of acid and neutralized (pH 5.5) lyophilized concentrated supernatants (cell-free supernatant; CFS) and whey (cell-free whey fractions; CFW) from de Man-Rogosa-Sharpe/trypticase-phytone-yeast broth and skim milk. Read More

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http://dx.doi.org/10.1080/13102818.2014.987450DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4434095PMC
January 2015
2 Reads

Selected adjunct cultures remarkably increase the content of bioactive peptides in Bulgarian white brined cheese.

Biotechnol Biotechnol Equip 2015 Jan 28;29(1):78-83. Epub 2014 Oct 28.

Department of Biotechnology, Faculty of Biology, Sofia University "St. Kliment Ohridski" , Sofia , Bulgaria.

Some lactic acid bacteria strains in milk media are capable of releasing bioactive peptides. In this study, we evaluated the angiotensin-converting enzyme (ACE)inhibitory activity of 180 lactic acid bacteria and selected several , subsp and strains that demonstrated strong ACE-inhibitory activity. The aim was to carry out a molecular study on the bioactive peptides released by the strains with the best ACE-inhibitory properties and by the strains demonstrating a calcium-binding effect. Read More

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http://dx.doi.org/10.1080/13102818.2014.969918DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4434043PMC
January 2015
7 Reads

Optimization of permeabilization process of yeast cells for catalase activity using response surface methodology.

Biotechnol Biotechnol Equip 2015 Jan 9;29(1):72-77. Epub 2015 Jan 9.

Department of Chemical and Biochemical Engineering, Faculty of Chemical Technology and Engineering, University of Technology and Life Sciences , Bydgoszcz , Poland.

Biotransformation processes accompanied by whole yeast cells as biocatalyst are a promising area of food industry. Among the chemical sanitizers currently used in food technology, hydrogen peroxide is a very effective microbicidal and bleaching agent. In this paper, permeabilization has been applied to yeast cells aiming at increased intracellular catalase activity for decomposed HO. Read More

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http://dx.doi.org/10.1080/13102818.2014.934986DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4434045PMC
January 2015