360,124 results match your criteria Biotechnology and Bioengineering [Journal]


Identification of biofilm formation and exoelectrogenic population structure and function with graphene/polyanliline modified anode in microbial fuel cell.

Chemosphere 2018 Nov 30;219:358-364. Epub 2018 Nov 30.

State Key Laboratory of Urban Water Resources and Environment, Harbin Institute of Technology, Harbin, 150090, China; Key Laboratory of Environmental Biotechnology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, 100085, PR China. Electronic address:

Improving anode configuration with polymer or nanomaterial modification is promising for enhancing microbial fuel cell performance. However, how anode modification affects biofilm development and electrogenic function remains poorly understood. In this study, the carbon cloth anode modified with polyaniline and reduced graphene oxide was successfully fabricated which obtained the highest power output. Read More

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November 2018

Targeted and untargeted LC-MS polyphenolic profiling and chemometric analysis of propolis from different regions of Croatia.

J Pharm Biomed Anal 2018 Dec 4;165:162-172. Epub 2018 Dec 4.

University of Rijeka, Department of Biotechnology, Centre for high-throughput technologies, Radmile Matejčić 2, 51000 Rijeka, Croatia. Electronic address:

Propolis is a complex biological matrix consisting mostly of plant resins and waxes, and in a small proportion of the herbal secondary metabolites, phenols. Phenols are components that are responsible for biological activities of propolis, however, their qualitative and quantitative composition is strongly influenced by climate and vegetation. Although studies on profiling of propolis samples from different countries have been carried out for some time propolis from Croatia is still not characterized till now. Read More

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December 2018

A novel pro-apoptotic role of zinc octacarboxyphthalocyanine in melanoma me45 cancer cell's photodynamic therapy (PDT).

J Photochem Photobiol B 2018 Dec 5;190:146-153. Epub 2018 Dec 5.

Biosystems Group, Institute of Automatic Control, Silesian University of Technology, Akademicka 16, 44-100 Gliwice, Poland; Biotechnology Centre, Silesian University of Technology, Krzywoustego 8, 44-100 Gliwice, Poland. Electronic address:

Zn-based phthalocyanine acts as drug or photosensitizer in photodynamic therapy (PDT) for the treatment of cancer cells. The activated zinc octacarboxyphthalocyanine (ZnPcOC) reacts with oxygen, to generate reactive oxygen species for the damage of melanoma cancer cells, Me45. This in vitro study aimed at investigating the cytotoxic effects of different concentrations of ZnPcOC activated with a diode laser (λ = 685 nm) on Me45, and normal human fibroblast cells, NHDF. Read More

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December 2018

Risk of Chlamydia abortus transmission via embryo transfer using in vitro produced early bovine embryos.

Theriogenology 2018 Dec 1;126:114-120. Epub 2018 Dec 1.

LUNAM University, Oniris, Nantes-Atlantic National College of Veterinary Medicine, Food Science and Engineering, Sanitary Security of Reproduction Biotechnology Unit, Nantes, France. Electronic address:

The objectives of this study were to determine (i) whether Chlamydia (C.) abortus would adhere to the intact zona pellucida (ZP-intact) of early in vitro produced bovine embryos; (ii) whether the bacteria would adhere to the embryos (ZP-free) after in vitro infection; and (iii) the efficacy of the International Embryo Transfer Society (IETS) washing protocol. The experimentation was made twice. Read More

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December 2018

Near-infrared light control of bone regeneration with biodegradable photothermal osteoimplant.

Biomaterials 2018 Dec 10;193:1-11. Epub 2018 Dec 10.

Institute of Biomedicine and Biotechnology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China; Department of Physics and Materials Science, City University of Hong Kong, Tat Chee Avenue, Kowloon, Hong Kong, China. Electronic address:

Mild heat stimulation can promote the restoration of bone defects but unfortunately, the delivery of exo-hyperthermy into human body is not efficient enough. In this study, mild heat-induced osteogenesis with high efficacy is demonstrated on an osteoimplant composed of black phosphorus nanosheets and poly(lactic-co-glycolic acid) (BPs@PLGA) with the participation of near-infrared (NIR) light irradiation. BPs@PLGA with only 0. Read More

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December 2018
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Effects of inosine 5'-monophosphate supplementation in high fishmeal and high soybean diets on growth, immune-related gene expression in gibel carp (Carassius auratus gibelio var. CAS Ⅲ), and its challenge against Aeromonas hydrophila infection.

Fish Shellfish Immunol 2018 Dec 11. Epub 2018 Dec 11.

State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, Hubei, 430072, PR China.

The present study was conducted to evaluate dietary inosine 5'-monophosphate (5'-IMP) on growth, immune genes expression and disease resistance against Aeromonas hydrophila in juvenile gibel carp (Carassius auratus gibelio var. CAS Ⅲ) (initial body weight: 7.48g). Read More

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December 2018

Effects of hypoxia-reoxygenation stress on mitochondrial proteome and bioenergetics of the hypoxia-tolerant marine bivalve Crassostrea gigas.

J Proteomics 2018 Dec 11. Epub 2018 Dec 11.

Department of Marine Biology, University of Rostock, Rostock, Germany; Department of Maritime Systems, Interdisciplinary Faculty, University of Rostock, Rostock, Germany.

Mitochondria are key intracellular targets of hypoxia-reoxygenation (H/R) stress due to their central role in generation of ATP and reactive oxygen species (ROS). Intertidal oysters Crassostrea gigas are adapted to frequent H/R cycles and maintain aerobic function despite frequent oxygen fluctuations. To gain insight into the molecular mechanisms of H/R tolerance, we assessed changes in mitochondrial respiration and (phospho)proteome of C. Read More

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December 2018

Identification of B cell epitopes enhanced by protein unfolding and aggregation.

Mol Immunol 2018 Dec 11;105:181-189. Epub 2018 Dec 11.

Lydia Becker Institute of Immunology and Inflammation, School of Biological Sciences, Faculty of Biology, Medicine and Health, Manchester Academic Health Science Centre, The University of Manchester, Oxford Road, Manchester, UK. Electronic address:

Aggregation of therapeutic proteins is a key factor in the generation of unwanted immunogenicity, and can result in reduced serum half-life, neutralization of function and adverse health effects. There is currently little information regarding how aggregates interact with B-cell receptors or cognate antibodies at the protein sequence level, or whether non-native, aggregate-induced epitopes predominate in these interactions. Using an antibody fragment (single chain antibody variable fragment; scFv) that forms aggregates readily at low temperature, anti-scFv IgG antibody responses were generated by intraperitoneal injection of BALB/c strain mice with monomer or aggregate preparations. Read More

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December 2018

Enhanced hypovirus transmission by engineered super donor strains of the chestnut blight fungus, Cryphonectria parasitica, into a natural population of strains exhibiting diverse vegetative compatibility genotypes.

Virology 2018 Dec 11;528:1-6. Epub 2018 Dec 11.

Division of Plant and Soil Sciences, West Virginia University, Morgantown, WV, USA. Electronic address:

Horizontal transmission of virulence attenuating hypoviruses of Cryphonectria parasitica is restricted by an allorecognition system termed vegetative incompatibility (vic). A super donor formulation of two engineered C. parasitica strains (SD328/SD82) with gene disruptions at four of six vic loci transmitted hypovirus to strains in the laboratory independent of vic genotype. Read More

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December 2018

Targeting STATs in neuroinflammation: the Road Less Traveled!

Pharmacol Res 2018 Dec 11. Epub 2018 Dec 11.

Department of Pharmacology, Faculty of Pharmacy, Kermanshah University of Medical Sciences, Kermanshah, Iran.

JAK/STAT transduction pathway is a highly conserved pathway implicated in regulating cellular proliferation, differentiation, survival and apoptosis. Dysregulation of this pathway is involved in the onset of autoimmune, haematological, oncological, metabolic and neurological diseases. Over the last few years, the research of anti-neuroinflammatory agents has gained considerable attention. Read More

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December 2018

Induction of protective immunity by recombinant peptidoglycan associated lipoprotein (rPAL) protein of Legionella pneumophila in a BALB/c mouse model.

Microb Pathog 2018 Dec 11. Epub 2018 Dec 11.

Department of Microbiology, Faculty of Advanced Sciences, Medical Sciences University Islamic Azad, Tehran, Iran.

Legionella pneumophila causes a severe form of pneumonia known as Legionnaires' disease especially in patients with impaired cellular immune response. In order to prevent the disease, immunogenicity and the level of the induction of protective immunity from the recombinant peptidoglycan-associated lipoprotein (rPAL) against Legionella pneumophila in BALB/c mice was examined. Mice immunized with (rPAL) rapidly increased an antibody response in serum and also displayed a strong activation of both innate and adaptive cell-mediated immunity as determined by antigen-specific splenocyte proliferation, an early production of pro-inflammatory cytokines in the serum and in the splenocyte cultures. Read More

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December 2018

Iron-associated protein interaction networks reveal the key functional modules related to survival and virulence of Pasteurella multocida.

Microb Pathog 2018 Dec 11. Epub 2018 Dec 11.

Department of Zoology, Faculty of Science, Kasetsart University, Bangkok, 10900, Thailand; Omics Center for Agriculture, Bioresources, Food, and Health, Faculty of Science, Kasetsart University (OmiKU), Bangkok, 10900, Thailand. Electronic address:

Pasteurella multocida causes respiratory infectious diseases in a multitude of birds and mammals. A number of virulence-associated genes were reported across different strains of P. multocida, including those involved in the iron transport and metabolism. Read More

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December 2018

Biochemical characterization of a native group III trypsin ZT from Atlantic cod (Gadus morhua).

Int J Biol Macromol 2018 Dec 11. Epub 2018 Dec 11.

Faculty of Food Science and Nutrition, School of Health Sciences, University of Iceland. University of Iceland, Sæmundargata 2, 101 Reykjavik, Iceland. Electronic address:

Atlantic cod trypsin ZT is biochemically characterized for the first time in this report in comparison to a group I trypsin (cod trypsin I). To our knowledge, trypsin ZT is the first thoroughly characterized group III trypsin. A more detailed understanding of trypsin ZT biochemistry may give insight into its physiological role as well as its potential use within the biotechnology sector. Read More

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December 2018

Virus and host interactions critical for filoviral RNA synthesis as therapeutic targets.

Antiviral Res 2018 Dec 11. Epub 2018 Dec 11.

Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, MO 63110, USA.

Filoviruses, which include Ebola virus and Marburg virus, are negative-sense RNA viruses associated with sporadic outbreaks of severe viral hemorrhagic fever characterized by uncontrolled virus replication. The extreme virulence and emerging nature of these zoonotic pathogens make them a significant threat to human health. Replication of the filovirus genome and production of viral RNAs requires the function of a complex of four viral proteins, the nucleoprotein (NP), viral protein 35 (VP35), viral protein 30 (VP30) and large protein (L). Read More

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December 2018

Identification and immune-related analysis of SNPs in Litopenaeus vannamei Toll3 receptor.

Immunol Lett 2018 Dec 11. Epub 2018 Dec 11.

Department of Biology and Guangdong Provincial Key Laboratory of Marine Biotechnology, Shantou University, Shantou, 515063, China. Electronic address:

Tolls and Toll-like receptors (TLRs), as innate immune-recognition receptors that recognize molecular patterns associated with microbial pathogens, play a critical role in antimicrobial immune responses. Here, we report on single nucleotide polymorphisms (SNPs) of Litopenaeus vannamei Toll3 (LvToll3). Multiple sequence alignment of the L. Read More

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December 2018

Genome survey sequencing for the characterization of genetic background of Dracaena cambodiana and its defense response during dragon's blood formation.

PLoS One 2018 14;13(12):e0209258. Epub 2018 Dec 14.

Key Laboratory of Biology and Genetic Resources of Tropical Crops of Ministry of Agriculture and Rural Affairs, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan, People's Republic of China.

Dragon's blood collected from the genus Dracaena is used as a renowned traditional medicine in various cultures worldwide. However, the genetics of the genus Dracaena and the formation mechanism of dragon's blood remain poorly understood. Here, we generate the first draft genome reference assembly of an elite Chinese Dracaena species, Dracaena cambodiana, from next-generation sequencing data with 89. Read More

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December 2018

Prediction of antibiotic resistance in Escherichia coli from large-scale pan-genome data.

PLoS Comput Biol 2018 Dec 14;14(12):e1006258. Epub 2018 Dec 14.

Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, Cambridgeshire, United Kingdom.

The emergence of microbial antibiotic resistance is a global health threat. In clinical settings, the key to controlling spread of resistant strains is accurate and rapid detection. As traditional culture-based methods are time consuming, genetic approaches have recently been developed for this task. Read More

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December 2018

Novel mass spectrometry based detection and identification of variants of rabies virus nucleoprotein in infected brain tissues.

PLoS Negl Trop Dis 2018 Dec 14;12(12):e0006984. Epub 2018 Dec 14.

Poxvirus and Rabies Branch, Centers for Disease Control and Prevention, Atlanta, GA, United States of America.

Human rabies is an encephalitic disease transmitted by animals infected with lyssaviruses. The most common lyssavirus that causes human infection is rabies virus (RABV), the prototypic member of the genus. The incubation period of RABV in humans varies from few weeks to several months in some instances. Read More

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December 2018

Functional and Transcriptomic Characterization of a Dye-decolorizing Fungus from Rhizosphere.

Pol J Microbiol 2018 ;67(4):417-430

Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences , Beijing , China.

We isolated three laccase-producing fungus strains from Taxus rhizosphere. Myrotheium verrucaria strain DJTU-sh7 had the highest laccase activity of 216.2 U/ml, which was increased to above 300 U/ml after optimization. Read More

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January 2018

[The study of patterns of development of resistance of Staphylococcus aureus to triclosan].

Klin Lab Diagn 2018 ;63(1):60-64

The Federal budget institution of science "The state scientifc center of applied microbiology and biotechnology" of the Rospotrebnadzor, 142279, Obolensk, Moscow oblast, Russia.

The article presents the results of studying molecular genetic mechanisms of development of resistance to antiseptic Triclosan in strain Staphylococcus aureus АТСС25923. The modifcations of strain S. aureus АТСС25923 (Tr1, Tr2, Tr1С и Tr2С) are obtained resistant to 64 mg/l of Triclosan and stably preserving the given characteristic under cultivation in absence of selective pressure. Read More

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January 2018

[The becoming of reactions of lipolysis in phylogenesis. The palmitic and oleic triglycerides as substrates. Insulin, condition of normolipemia and formation of hyper lipoproteinemia type IIb, IV and V].

Klin Lab Diagn 2018 ;63(1):4-15

The Federal state budget scientifc institution "The research institute of general pathology and pathophysiology" of the Russian academy of sciences, 125315, Moscow, Russia.

According to phylogenetic theory of general pathology, when living in ocean all were carnivorous (piscivorous) fatty acids transferring to cells in form of non-polar triglycerides nitially began apoB-48 chylomicrons, continued lipoproteins of very low and low density and fnalized its apoB-100 endocytosis. The fatty acids are transferred by chylomicrons + lipoproteins of very low density + lipoproteins of low density and non-polar triglycerides are hydrolyzed by hepatic glycerolhydrogenase and co-enzyme apoC-III; according WHO classifcation, hyperlipoproteinemia corresponds to type V. On land, in herbivorous who are not yet synthesized insulin, apoB-48 and chylomicrons left process of non-polar triglycerides transferring. Read More

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January 2018

Role and regulation of proapoptotic Bax in oral squamous cell carcinoma and drug resistance.

Head Neck 2018 Dec 14. Epub 2018 Dec 14.

Centre for Life Sciences, School of Natural Sciences, Central University of Jharkhand, Ranchi, Jharkhand, India.

Background: Bax, a proapoptotic protein but its regulation during oral cancer progression and resistance remains elusive.

Methods: A total of 127 samples including adjacent normal, primary tumor, and resistance to chemoradiation therapy (RCRT) samples from oral squamous cell carcinoma (OSCC) patients were used. The status of Bax was analyzed at DNA/mRNA/protein levels and the results were correlated with p53 and Akt expression in tissue samples/cisplatin-resistant oral tongue SCC (SCC9/SCC4-CisR) cell line. Read More

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December 2018

DNA damage signatures in peripheral blood cells (PBMC) as biomarkers in prodromal Huntington's disease.

Ann Neurol 2018 Dec 14. Epub 2018 Dec 14.

Department of Biology, University of Naples "Federico II", Naples, Italy.

Easily accessible biomarkers in Huntington disease (HD) are actively searched. We investigated telomere length (TL) and DNA double-strand breaks (histone variant pγ-H2AX) as predictive disease biomarkers in peripheral blood mononuclear cells (PBMC) from 25 pre-manifest, 58 HD patients, with similar CAG expansion in the huntingtin gene (HTT) gene and 44 healthy controls (HC). PBMC from PRE-HD and HD groups showed shorter telomeres (p<0. Read More

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December 2018

Detection of an HLA-C*03 variant, HLA-C*03:258, in a Taiwanese individual.

Authors:
K L Yang P Y Lin

HLA 2018 Dec 13. Epub 2018 Dec 13.

Laboratory of Immunogenetics, Tzu Chi Cord Blood Bank, and Buddhist Tzu Chi Bone Marrow Donor Registry, Buddhist Tzu Chi Stem Cells Centre, Hualien Tzu Chi Hospital, Hualien, Taiwan.

One nucleotide substitution at residue 506 of HLA-C*03:02:01 results in a novel allele, HLA-C*03:258. This article is protected by copyright. All rights reserved. Read More

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December 2018

Tetrandrine inhibits human brain glioblastoma multiforme GBM 8401 cancer cell migration and invasion in vitro.

Environ Toxicol 2018 Dec 13. Epub 2018 Dec 13.

Department of Biological Science and Technology, China Medical University, Taichung, Taiwan.

Tetrandrine (TET) has been reported to induce anti-cancer activity in many human cancer cells and also to inhibit cancer cell migration and invasion. However, there are no reports to show TET inhibits cell migration and invasion in human brain glioblastoma multiforme GBM 8401 cells. In this study, we investigated the anti-metastasis effects of TET on GBM 8401 cells in vitro. Read More

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December 2018

Climate change impact and adaptation for wheat protein.

Glob Chang Biol 2019 Jan 22;25(1):155-173. Epub 2018 Nov 22.

Institute of Crop Science and Resource Conservation INRES, University of Bonn, Bonn, Germany.

Wheat grain protein concentration is an important determinant of wheat quality for human nutrition that is often overlooked in efforts to improve crop production. We tested and applied a 32-multi-model ensemble to simulate global wheat yield and quality in a changing climate. Potential benefits of elevated atmospheric CO concentration by 2050 on global wheat grain and protein yield are likely to be negated by impacts from rising temperature and changes in rainfall, but with considerable disparities between regions. Read More

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January 2019

The interaction of Chitosan and BMP-2 tuned by deacetylation degree and pH value.

J Biomed Mater Res A 2018 Dec 13. Epub 2018 Dec 13.

Key Lab of Advanced Technologies of Materials, Ministry of Education, School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu, 610031, Sichuan, China.

Integrating proteins with chitosan (CS) can not only improve the biocompatibility of CS, but also enable the sustained release of proteins. The deacetylation degree (DD) and pH conditions are considered as the two most important factors affecting the interaction between CS and proteins. In this study, the effects of CS with different DDs and pH conditions on bone morphogenetic protein-2 (BMP-2) adsorption were evaluated using the molecular dynamics (MD) method. Read More

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December 2018

Developmental neurotoxicity and immunotoxicity induced by graphene oxide in zebrafish embryos.

Environ Toxicol 2018 Dec 13. Epub 2018 Dec 13.

College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, China.

Graphene oxide (GO) has emerged as the worldwide promising candidate for biomedical application, such as for drug delivery, bio-sensing and anti-cancer therapy. This study was focused on the zebrafish and RAW264.7 cell line as in vivo and in vitro models to assess the potential developmental neurotoxicity and immunotoxicity of GO. Read More

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December 2018

Chalcone flavokawain A attenuates TGF-β1-induced fibrotic pathology via inhibition of ROS/Smad3 signaling pathways and induction of Nrf2/ARE-mediated antioxidant genes in vascular smooth muscle cells.

J Cell Mol Med 2018 Dec 13. Epub 2018 Dec 13.

Institute of Nutrition, College of Biopharmaceutical and Food Sciences, China Medical University, Taichung, Taiwan.

TGF-β1 plays a crucial role in the pathogenesis of vascular fibrotic diseases. Chalcones are reportedly cancer chemo-preventive food components that are rich in fruits and vegetables. In this study, flavokawain A (FKA, 2-30 μM), a naturally occurring chalcone in kava extracts, was evaluated for its anti-fibrotic and antioxidant properties in TGF-β1-stimulated vascular smooth muscle (A7r5) cells, as well as its underlying molecular mechanism of action. Read More

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December 2018

Integrating a genome-wide association study with a large-scale transcriptome analysis to predict genetic regions influencing the glycaemic index and texture in rice.

Plant Biotechnol J 2018 Dec 13. Epub 2018 Dec 13.

International Rice Research Institute, Los Baños, Laguna, 4030, Philippines.

Reliably generating rice varieties with low glycaemic index (GI) is an important nutritional intervention given the high rates of Type II diabetes incidences in Asia where rice is staple diet. We integrated a genome-wide association study (GWAS) with a transcriptome-wide association study (TWAS) to determine the genetic basis of the GI in rice. GWAS utilized 305 re-sequenced diverse indica panel comprising ~2. Read More

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December 2018

Surface Attachment Enhances the Thermodynamic Stability of Protein L.

Angew Chem Int Ed Engl 2018 Dec 13. Epub 2018 Dec 13.

University of California Santa Barbara, Department of Chemistry and Biochemistry, Dept. Chem & Biochem, UCSB, 93106, Santa Barbara, UNITED STATES.

Despite the importance of protein-surface interactions in both biology and biotechnology, our understanding of their origins is limited due to a paucity of experimental studies of the thermodynamics behind such interactions. In response we have characterized the extent to which interaction with a chemically well-defined macroscopic surface alters the stability of protein L. To do so, we site-specifically attached a redox-reporter-modified protein variant to a hydroxyl-terminated monolayer on a gold surface and then used electrochemistry to monitor its guanidine denaturation and determine its folding free energy. Read More

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December 2018

Reductive performance of ZVI/Cu polyscale particle to decolorize reactive black 5.

Microsc Res Tech 2018 Dec 14. Epub 2018 Dec 14.

Key Lab of Science and Technology of Eco-textile, Ministry of Education, College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai, China.

Based on direct reducing of copper ions by commercially available zero valent iron (ZVI), ZVI/Cu polyscale particles with high activation are synthesized and exhibit significant removal efficiency for reactive black (RB) dye. The ZVI/Cu polyscale particles could maintain a good performance for RB removal at a wide pH range of 3.0-9. Read More

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December 2018

Polyelemental Nanolithography via Plasma Ion Bombardment: From Fabrication to Superior H Sensing Application.

Adv Mater 2018 Dec 14:e1805343. Epub 2018 Dec 14.

Department of Chemical and Biomolecular Engineering (BK-21 Plus), Korea Advanced Institute of Science and Technology (KAIST), Yuseong-gu, Daejeon, 34141, Republic of Korea.

The development of complex nanostructures containing a homo- and heteromixture of two or more metals is a considerable challenge in nanotechnology. However, previous approaches are considerably limited to the number of combinations of metals depending on the compatibility of elements, and to the complex shape control of the nanostructure. In this study, a significant step is taken toward resolving these limitations via the utilization of a low-energy argon-ion bombardment. Read More

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December 2018

EgRBP42 from oil palm enhances adaptation to stress in Arabidopsis through regulation of nucleocytoplasmic transport of stress-responsive mRNAs.

Plant Cell Environ 2018 Dec 13. Epub 2018 Dec 13.

Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia.

Abiotic stress reduces plant growth and crop productivity. However, the mechanism underlying post-transcriptional regulations of stress response remains elusive. Herein, we report the post-transcriptional mechanism of nucleocytoplasmic RNA transport of stress-responsive transcripts mediated by EgRBP42, an hnRNP-like RNA-binding protein from oil palm, which could be necessary for rapid protein translation to confer abiotic stress tolerance in plants. Read More

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December 2018

Enhancing the oral bioavailability of baicalein via Solutol HS15 and Poloxamer 188 mixed micelles system.

J Pharm Pharmacol 2018 Dec 14. Epub 2018 Dec 14.

Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing, China.

Objectives: To increase the solubility of baicalein (BAI) by preparing BAI-micelles (BAI-M) with Solutol HS15 (HS15) and Poloxamer 188 (F68), thereby improving its oral bioavailability.

Methods: Baicalein micelles were prepared with HS15 and F68 by thin-film dispersion method and optimized by central composite design (CCD) approach. Physicochemical, in vitro release, Caco-2 cell transport and pharmacokinetic studies of BAI-M were performed. Read More

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December 2018

Myocardial Cell Signaling During the Transition to Heart Failure: Cellular Signaling and Therapeutic Approaches.

Compr Physiol 2018 Dec 13;9(1):75-125. Epub 2018 Dec 13.

St. Boniface Hospital Research Centre, Department of Physiology and Pathophysiology, University of Manitoba, Winnipeg, Canada.

Cardiovascular disease leading to heart failure (HF) remains a leading cause of morbidity and mortality worldwide. Improved pharmacological and interventional coronary procedures have led to improved outcomes following acute myocardial infarction. This success has translated into an unforeseen increased incidence in HF. Read More

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December 2018

Cell wall traits that influence plant development, immunity and bioconversion.

Plant J 2018 Dec 13. Epub 2018 Dec 13.

Department of Biology and Biotechnology "C. Darwin", Sapienza University of Rome, Piazzale A. Moro, 00185, Roma, Italy.

The architecture of the plant cell wall is highly dynamic, being substantially re-modelled during growth and development. Cell walls determine size and shape of cells and contribute to the functional specialization of tissues and organs. Beyond the physiological dynamics, the wall structure undergoes changes upon biotic or abiotic stresses. Read More

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December 2018

Anti-psoriatic effect of myeloid-derived suppressor cells on imiquimod-induced skin inflammation in mice.

Scand J Immunol 2018 Dec 13:e12742. Epub 2018 Dec 13.

Pharmicell Co, Ltd, 484, Dunchon-daero, Jungwon-gu, Seongnam, Gyeonggi-do, Korea.

Myeloid-derived suppressor cells (MDSCs) play an important role in controlling the immune response against cancer and in suppression of autoimmunity and allergic inflammation. However, the beneficial effects of MDSCs on the experimental mouse model of psoriasis have not been reported. Therefore, we investigated the anti-psoriatic effect of MDSCs on IMQ-induced skin inflammation in mice and explored the mechanisms involved. Read More

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December 2018

No Lanthanides-based Catalysis in Eukaryotes.

IUBMB Life 2018 Dec 13. Epub 2018 Dec 13.

Interdepartmental Laboratory for Electron Microscopy, Roma Tre University, Roma, Italy.

In the "Hypothesis" paper entitled "Lanthanides-Based Catalysis in Eukaryotes" (IUBMB Life 2018 Nov;70(11):1067-1075), we analyzed the possibility that Ce -dependent methanol dehydrogenases (MDHs) could be found not only in archaea and bacteria but also in eukaryotic organisms. This hypothesis was based on the observation that MDHs protein sequences carrying the signature of Ce -based active sites could be found in genome-derived proteomes of the eukaryotes Plasmodium yoelii yoelii, Nephila clavipes, Hyalella azteca, Pantholops hodgsonii, and Homo sapiens. Data were analyzed following standard procedures employed in the study of phylogenetic relationships among members of protein families and their occurrence in diverse organisms. Read More

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December 2018

Effect of BMP15 and/or AMH during in Vitro Maturation of Oocytes from Involuntarily Culled Dairy Cows.

Mol Reprod Dev 2018 Dec 13. Epub 2018 Dec 13.

Laboratory of Animal Biotechnology, Department of Animal Science, Faculty of Veterinary Sciences, University of Concepcion, Chillán, Chile.

The high metabolic activity to which the dairy cattle are exposed for maintain milk production altered steroid metabolism that affect reproductive physiology and reducing oocyte competence. Our aims were 1) to characterize the competence of immature oocytes collected from dairy cattle based on the expression of genes in cumulus cells and 2) to improve oocyte competence to support pre-implantation embryo development by the supplementation of maturation medium with BMP15 and/or AMH. Oocyte donors were identified at the moment of ovary collection and grouped by involuntarily culled dairy cows (Holstein breed) or beef cattle. Read More

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December 2018

LC/MS detection of oligogalacturonic acids obtained from tragacanth degradation by pectinase producing bacteria.

J Basic Microbiol 2018 Dec 13. Epub 2018 Dec 13.

School of Chemical and Biological Engineering, Seoul National University, Gwanak-gu, Seoul, Republic of Korea.

Tragacanth, a highly branched carbohydrate polymer isolated from Astragalus, is one of the most commonly used gums in food industry. The primary structure of tragacanth is composed of galacturonic acid monomers connected with α 1-4 links, and it is very similar to the pectin. Tragacanth degradation by microorganisms is significant in two aspects: first, food preservation and microbial growth control due to too much use of tragacanth in the food industry, second, therapeutic and pharmaceutical potential of obtained oligosaccharides. Read More

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December 2018

The role of inflammasome complex in ischemia-reperfusion injury.

J Cell Biochem 2018 Dec 13. Epub 2018 Dec 13.

Health research institute,, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.

Ischemia-reperfusion injury refers to a temporary interruption of blood flow in a tissue. Restoration of blood flow initiates the inflammation in tissue causing ischemic damage through the activation of a multiprotein complex termed inflammasome. The complex contains a receptor, mainly a member of nucleotide oligomerization domain-like receptors, that receives danger signals. Read More

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December 2018

MMP-14 single-nucleotide polymorphisms are related to steroid-induced osteonecrosis of the femoral head in the population of northern China.

Mol Genet Genomic Med 2018 Dec 12. Epub 2018 Dec 12.

The 2nd Affiliated Hospital of Inner Mongolia Medical University, Hohhot, China.

Background: Osteonecrosis of the femoral head (ONFH) is a refractory disease which frequently occurs in young and middle-aged people. Recent studies indicated that MMP-14 played an important role in the development of chondrocytes, metabolism of osteoblasts as well as fate decision of hypertrophic chondrocytes. The aim of this study was to investigate the association between polymorphisms of MMP-14 and steroid-induced osteonecrosis of the femoral head in the Chinese population. Read More

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December 2018

The construction of an in vitro synthetic enzymatic biosystem that facilitates laminaribiose biosynthesis from maltodextrin and glucose.

Biotechnol J 2018 Dec 13:e1800493. Epub 2018 Dec 13.

Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 32 West 7th Avenue, Tianjin Airport Economic Area, Tianjin 300308, People's Republic of China.

Laminaribiose is a reducing glucobiose linked by a β-1,3 glycosidic bond; it is also a precursor for building blocks in the pharmaceutical industry, a powerful germinating agent and antiseptic, as well as a potential prebiotic. In this study, an in vitro enzymatic biosystem composed of α-glucan phosphorylase, laminaribiose phosphorylase, isoamylase and 4-glucanotransferase was designed for the one-pot synthesis of laminaribiose from low-cost maltodextrin and glucose. Through condition optimization, 51 mM laminaribiose was produced from 10 g/L maltodextrin (55. Read More

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December 2018

Toxicological evaluation of metal oxide nanoparticles and mixed exposures at low doses using zebra fish and THP1 cell line.

Environ Toxicol 2018 Dec 12. Epub 2018 Dec 12.

Department of Biotechnology, University Institute of Engineering and Technology (UIET), Panjab University, Chandigarh, India.

Metal and metal oxide nanoparticles are being used in different industries now-a-days leading to their unavoidable exposure to humans and animals. In the present study, toxicological testing was done using nanoparticles of copper oxide, cerium oxide and their mixture (1:1 ratio) on zebra fish embryos and THP-1 cell line. Zebrafish embryos were exposed to 0. Read More

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December 2018

Bacteria and oxidative stress: An annotated selection of World Wide Web sites relevant to the topics in environmental microbiology.

Environ Microbiol 2018 Dec;20(12):4629-4630

McKnight Professor, Microbial Biochemistry & Biotechnology, Department of Biochemistry, Molecular Biology & Biophysics, University of Minnesota, St. Paul, MN, 55108, USA.

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December 2018

Etiology of acute viral respiratory infections common in Pakistan: A review.

Rev Med Virol 2018 Dec 12:e2024. Epub 2018 Dec 12.

Department of Bioinformatics and Biotechnology, International Islamic University, Islamabad, Pakistan.

Respiratory infections, especially those of the lower respiratory tract, remain a foremost cause of mortality and morbidity of children greater than 5 years in developing countries including Pakistan. Ignoring these acute-level infections may lead to complications. Particularly in Pakistan, respiratory infections account for 20% to 30% of all deaths of children. Read More

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December 2018

Structural and biochemical evaluation of Ceratitis capitata OBP22 affinity for odorants involved in inter-sex communication.

Insect Mol Biol 2018 Dec 12. Epub 2018 Dec 12.

Department of Biology and Biotechnology "L. Spallanzani", University of Pavia, 27100, Pavia, Italy.

In insects, odorant-binding proteins (OBPs) connect the peripheral sensory system to receptors of olfactory organs. Medfly Ceratitis capitata CcapObp22 shows 37% identity and close phylogenetic affinities with Drosophila melanogaster OBP69a/PB-PRP1 (pheromone-binding protein related protein 1). The CcapObp22 gene is transcribed in the antennae and maxillary palps, suggesting an active role in olfaction. Read More

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December 2018

Kernel size-related genes revealed by an integrated eQTL analysis during early maize kernel development.

Plant J 2018 Dec 12. Epub 2018 Dec 12.

Biotechnology Research Institute.

In maize, kernel traits strongly impact overall grain yields, and it is known that sophisticated spatiotemporal programs of gene expression coordinate kernel development, so advancing our knowledge of kernel development can help efforts to improve grain yields. Here, using phenotype, genotype, and transcriptomics data of maize kernels at 5 and 15 days after pollination (DAP) for a large association mapping panel, we employed multiple quantitative genetics approaches-genome wide association studies (GWAS) as well as expression quantitative trait loci (eQTL) and quantitative trait transcript (QTT) analyses-to gain insights about molecular genetic basis of kernel development in maize. This resulted in the identification of 137 putative kernel length-related genes at 5 DAP, of which 43 are located in previously reported QTL regions. Read More

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December 2018

Parentage assignment with genotyping-by-sequencing data.

J Anim Breed Genet 2018 Dec 13. Epub 2018 Dec 13.

The Roslin Institute and Royal (Dick) School of Veterinary Studies, The University of Edinburgh, Midlothian, UK.

In this paper, we evaluate using genotype-by-sequencing (GBS) data to perform parentage assignment in lieu of traditional array data. The use of GBS data raises two issues: First, for low-coverage (e.g. Read More

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December 2018