2,144 results match your criteria Biotechnology and Applied Biochemistry[Journal]


Utilization of prickly pear waste for baker's yeast production.

Biotechnol Appl Biochem 2019 Apr 17. Epub 2019 Apr 17.

Laboratory Reactions and Process Engineering (LRPE), UMR CNRS 7224, University of Lorraine, ENSAIA, Vandoeuvre Cedex, 54505, France.

The feasibility of baker's yeast production using fruits and peels of Opuntia ficus indica (OFI) as carbohydrate feedstock was investigated. Two response surface methodologies involving central composite face centered design (CCFD) were successfully applied. The effects of four independent variables on baker's yeast production from OFI fruit juice was evaluated using the first CCFD. Read More

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http://dx.doi.org/10.1002/bab.1753DOI Listing
April 2019
2 Reads

Cilostazol protects against myocardial ischemia and reperfusion injury by activating transcrption factor EB (TFEB).

Biotechnol Appl Biochem 2019 Apr 17. Epub 2019 Apr 17.

Department of Cardiology, The Affiliated Huaian No.1 People's Hospital of Nanjing Medical University.

Although cilostazol was proved to have anti-tumor biological effects. its function in myocardial ischemia and reperfusion (I/R) injury and the underlying mechanisna were not fully illustrated yet. In this study, a rat model of I/R injurywas constructed and qRT-PCR, Western blot and Immunofluorescence (IF) assay were performed. Read More

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https://onlinelibrary.wiley.com/doi/abs/10.1002/bab.1754
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http://dx.doi.org/10.1002/bab.1754DOI Listing
April 2019
1 Read

Preparation and Characterization of Curcumin-Loaded Polymeric Nanomicelles to Interference with Amyloidogenesis through Glycation Method.

Biotechnol Appl Biochem 2019 Apr 16. Epub 2019 Apr 16.

Bioengineering Research Group, Nanotechnology and Advanced Materials Department, Materials and Energy Research Center (MERC), P.O. Box 14155-4777, Tehran, Iran.

Amyloid fibrils, including β-amyloid (Aβ) fibrils, are protein aggregates that form under certain conditions, associated with neurodegeneration that interfere with neural synaptic transmission resulting in some neural disorders, such as Alzheimer disease. The aim of this study is to inhibit amyloidogenesis by using preparatory polymeric nanomicelles as therapeutic agents and also as nano carriers for curcumin to target Aβ fibrils through the glycation method of Bovine Serum Albumin (BSA) in the presence of Phosphate Buffer Saline (PBS). Polymeric nanomicelles were prepared from Phosphatidylethanolamine-distearoyl methoxypolyethylene glycol conjugates in the presence and absence of Curcumin and then the morphological and structural characteristics of the nanomicelles were characterized in detail. Read More

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https://onlinelibrary.wiley.com/doi/abs/10.1002/bab.1751
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http://dx.doi.org/10.1002/bab.1751DOI Listing
April 2019
2 Reads

Identification of nacre matrix protein genes hic14 and hic19 and their roles in crystal growth and pearl formation in the mussel Hyriopsis cumingii.

Biotechnol Appl Biochem 2019 Apr 16. Epub 2019 Apr 16.

Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture, Shanghai, 201306, China.

Biological mineralization is a highly programmed process in which inorganic minerals reassociate under the strict control of the extracellular matrix to form minerals with special functions and patterns. Shells are bio-minerals, and their synthesis is finely regulated by organic matrix including matrix proteins, polysaccharides, lipids, pigments, free amino acids and small peptides. In this study, two matrix protein genes, hic14 and hic19, were isolated from the mantle of the mussel Hyriopsis cumingii. Read More

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http://dx.doi.org/10.1002/bab.1752DOI Listing

Improving the cost effectiveness of enhanced green fluorescent protein production using recombinant Escherichia coli BL21 (DE3): Decreasing the expression inducer concentration.

Biotechnol Appl Biochem 2019 Apr 8. Epub 2019 Apr 8.

Department of Bioprocesses and Biotechnology, School of Pharmaceutical Sciences, São Paulo State University (UNESP), Araraquara, Brazil.

Green fluorescent protein (GFP) is a globular protein used as biosensor and biomarker in medical and industrial fields. However, due to the expensive production costs of expressing proteins using high-cost inducers like isopropyl-β-d-1-thiogalactopyranoside (IPTG), the number of GFP applications are still scarce. This work studied the production of enhanced GFP (EGFP) using Escherichia coli BL21 (DE3) [pLysS; pET28(a)], aiming to increase its yield and reduce costs. Read More

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http://dx.doi.org/10.1002/bab.1749DOI Listing
April 2019
1 Read

A review: Nanofibrous scaffold in possible prevention and treatment of coronary artery disease.

Biotechnol Appl Biochem 2019 Apr 6. Epub 2019 Apr 6.

Department of Cardiology, Lorestan University of Medical Sciences, Khoramabad, Iran.

Nanofibrous scaffolds have potential to improve coronary stent applications by promoting endothelial recovery on the stent surface and aids regeneration of cardiac tissues. Presently, scaffolds fabricated via electro-spinning are been widely used because of their ability to bio-mimic the precise anatomical structure of the protein fibers. Properties like convenience to spin on several components and functionalization with several bioactive molecules have signify the use of nanofibrous scaffolds for tissue engineering. Read More

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http://dx.doi.org/10.1002/bab.1750DOI Listing
April 2019
5 Reads

Improved production of antifungal angucycline Sch47554 by manipulating three regulatory genes in Streptomyces sp. SCC-2136.

Biotechnol Appl Biochem 2019 Apr 1. Epub 2019 Apr 1.

Department of Biological Engineering, Utah State University, Logan, UT, USA.

Sch47554 and Sch47555 are two angucyclines with antifungal activities against various yeasts and dermatophytes from Streptomyces sp. SCC-2136. The sch gene cluster contains several putative regulatory genes. Read More

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http://dx.doi.org/10.1002/bab.1748DOI Listing

Development and identification of a fully human single-chain variable fragment 29 against TSLP.

Biotechnol Appl Biochem 2019 Mar 28. Epub 2019 Mar 28.

The School of Basic Medical Sciences, Southwest Medical University, Luzhou, Sichuan Province, People's Republic of China.

Thymic stromal lymphopoietin is a key initiator for inducing Th2-type inflammation and a potential therapeutic target for allergic disease. In the present study, the naive human antibody library was enriched using human thymic stromal lymphopoietin (hTSLP) as an antigen by phage display. Single clones were randomly picked from the enriched antibody library after three rounds of selection, and these were expressed for enzyme-linked immunosorbent assay (ELISA). Read More

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http://dx.doi.org/10.1002/bab.1747DOI Listing
March 2019
1.362 Impact Factor

A novel sensitive laccase biosensor using gold nanoparticles and poly L-arginine to detect catechol in natural water.

Biotechnol Appl Biochem 2019 Mar 27. Epub 2019 Mar 27.

Faculty of Chemistry, Razi University, Kermanshah, Iran.

In this study, the simple, green, and fast layer-by-layer modification of the glassy carbon electrode was mainly performed by electrodeposition of gold nanoparticles and then, poly-l-arginine, and finally, laccase was covalently bonded to poly-l-arginine using glutaraldehyde. This type of fabrication is used for the first time for catechol detection, which provides a bioelectrocatalytic cycle for electron transport in the presence of laccase that results in sensitive and fast detection of catechol. The scanning electron microscopy, Fourier-transform infrared spectroscopy, and electrochemical studies were performed to confirm successful immobilization of the enzyme. Read More

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http://dx.doi.org/10.1002/bab.1746DOI Listing

Optimization, purification, and characterization of hydroxylamine oxidoreductase from Acinetobacter sp. Y1.

Biotechnol Appl Biochem 2019 Mar 24. Epub 2019 Mar 24.

Key Laboratory of Coal Science and Technology of Shanxi Province and Ministry of Education, Taiyuan University of Technology, Taiyuan, Shanxi, People's Republic of China.

Hydroxylamine oxidoreductase (HAO) is a key enzyme involved in ammonium removal pathway. To further study the enzyme, HAO was purified from heterotrophic nitrifier Acinetobacter sp. Y1 and its property was investigated. Read More

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http://dx.doi.org/10.1002/bab.1745DOI Listing
March 2019
9 Reads

Activation of Nrf2 signaling by Icariin protects against 6-OHDA-induced neurotoxicity.

Biotechnol Appl Biochem 2019 Mar 20. Epub 2019 Mar 20.

Key Laboratory of Basic Pharmacology of Ministry of Education and Joint International Research Laboratory of Ethnomedicine of Ministry of Education, Zunyi Medical University, Zunyi, Guizhou, People's Republic of China.

Nerve damage is the main pathogenesis of neurodegenerative diseases. Recently, in search for a promising therapeutic target that could stop neurodegenerative diseases progression, the antioxidant signaling pathway regulated by transcription factor nuclear factor erythroid 2-related factor 2 (Nrf2) has attracted new hopes. Icariin (ICA) exhibited a battery of pharmacological properties, including antioxidation, anti-aging, and anti-inflammation activities. Read More

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https://onlinelibrary.wiley.com/doi/abs/10.1002/bab.1743
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http://dx.doi.org/10.1002/bab.1743DOI Listing
March 2019
3 Reads

A comprehensive review on staphylococcal protein A (SpA): Its production and applications.

Biotechnol Appl Biochem 2019 Mar 14. Epub 2019 Mar 14.

Associate Professor, Department of Industrial and Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran.

The Staphylococcus aureus protein A (SpA) can be obtained through the culture of wild-type S. aureus and also as a recombinant protein in safe bacterial hosts. Several methods have been used to purify SpA among which ion-exchange chromatography, affinity chromatography, gel filtration, and per aqueous liquid chromatography (PALC) are common. Read More

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http://dx.doi.org/10.1002/bab.1742DOI Listing
March 2019
1 Read
1.362 Impact Factor

A conductive cell-imprinted substrate based on CNT-PDMS composite.

Biotechnol Appl Biochem 2019 Feb 28. Epub 2019 Feb 28.

National Cell Bank of Iran, Pasteur Institute of Iran, Tehran, Iran.

Cell function regulation is influenced by continuous biochemical and biophysical signal exchange within the body. Substrates with nano/micro-scaled topographies that mimic the physiological niche are widely applied for tissue engineering applications. As the cartilage niche is composed of several stimulating factors, a multifunctional substrate providing topographical features while having the capability of electrical stimulation is presented. Read More

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http://dx.doi.org/10.1002/bab.1741DOI Listing
February 2019
1 Read

Facile reflux synthesis of polyethyleneimine-capped fluorescent carbon dots for sequential bioassays toward Cu /H S and its application for a logic system.

Biotechnol Appl Biochem 2019 Feb 22. Epub 2019 Feb 22.

School of Chemistry and Molecular Engineering, Shanghai Key Laboratory for Urban Ecological Processes and Eco-Restoration, East China Normal University, Shanghai, People's Republic of China.

In this work, we present a novel facile strategy for green synthesis of polyethyleneimine (PEI)-capped carbon dots (PEI-CDs), in which citric acid and PEI were chosen as reactants and highly fluorescent PEI-CDs could be readily obtained via a simple one-pot refluxing under 120 °C within 2 H. Fluorescence studies indicate that the as-prepared PEI-CDs exhibit strong fluorescence emission at 446 nm with excitation at 365 nm. Upon the sequential addition of Cu and H S, PEI-CDs result in an interesting "ON-OFF-ON" three-state emission responses, promising a bifunctional sensory platform. Read More

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http://dx.doi.org/10.1002/bab.1739DOI Listing
February 2019
1 Read

Ponicidin as a promising anticancer agent: Its biological and biopharmaceutical profile along with a molecular docking study.

Biotechnol Appl Biochem 2019 Feb 25. Epub 2019 Feb 25.

Department of Chemistry, The University of Jordan, Amman, Jordan.

Ponicidin, an ent-kaurane diterpenoid derived from Rabdosia rubescens, exhibits antitumor activities against several types of cancers. This review summarizes the botanical sources, biological activities, and biopharmaceutical profile of ponicidin. Additionally, a molecular docking study has been undertaken to correlate the interaction of this diterpenoid with biomacromolecules found in the literature. Read More

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http://dx.doi.org/10.1002/bab.1740DOI Listing
February 2019
2 Reads

Production of inulin- and neolevan-type fructooligosaccharides by Penicillium janczewskii Zaleski CCIBt 3352.

Biotechnol Appl Biochem 2019 Feb 13. Epub 2019 Feb 13.

Laboratório de Biologia, Prograd, Universidade Federal do ABC, Santo André, Brazil.

Fructooligosaccharides (FOS) are fructose-based oligosaccharides employed as additives to improve the nutritional and technological properties of foods. The rhizosphere of inulin-accumulating plants from the Cerrado (Brazilian savanna) harbor fungi capable of synthesizing FOS from sucrose through the transfructosylating activity of β-fructosyltransferases and/or β-fructofuranosidases. Here, we investigated the ability of Penicillium janczewskii Zaleski CCIBt 3352, a fungus isolated from the rhizosphere of Chrysolaena obovata (Asteraceae), to produce FOS in a medium supplemented with sucrose concentrations of 30, 100, or 150 g L . Read More

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http://dx.doi.org/10.1002/bab.1738DOI Listing
February 2019
1 Read

Selection and characterization of novel DNA aptamer against colorectal carcinoma Caco-2 cells.

Biotechnol Appl Biochem 2019 Feb 12. Epub 2019 Feb 12.

Department of Pharmacology, School of Medicine, Zhejiang University, Hangzhou, People's Republic of China.

Aptamers are short, single-stranded nucleic acid (DNA or RNA) oligonucleotides that can be obtained by a technique called systematic evolution of ligands by exponential enrichment (SELEX) in vitro. Due to superior properties such as small size, high binding affinity, and stability, they are considered to be feasible tools for diagnosis and treatment of disease. In the current study, we attempted to screen a high-affinity DNA aptamer to selectively target the colorectal carcinoma Caco-2 cells by using cell-based SELEX approach. Read More

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http://dx.doi.org/10.1002/bab.1737DOI Listing
February 2019
1 Read

Jasmonic acid-responsive AabHLH1 positively regulates artemisinin biosynthesis in Artemisia annua.

Biotechnol Appl Biochem 2019 Feb 4. Epub 2019 Feb 4.

Joint International Research Laboratory of Metabolic and Developmental Sciences, Key Laboratory of Urban Agriculture (South) Ministry of Agriculture, Plant Biotechnology Research Center, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, 200240, China.

Artemisia annua is the only natural source of the sesquiterpenoid artemisinin, which is widely used to treat malaria. The phytohormone jasmonic acid (JA) can significantly promote artemisinin biosynthesis in A. annua. Read More

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http://dx.doi.org/10.1002/bab.1733DOI Listing
February 2019
1 Read
1.362 Impact Factor

High-proline proteins in experimental hazy white wine produced from partially botrytized grapes.

Biotechnol Appl Biochem 2019 Feb 4. Epub 2019 Feb 4.

Department of Protein Biochemistry and Proteomics, Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacký University, Olomouc, Czech Republic.

Undesirable effects of the pathogen Botrytis cinerea include reduced quality and quantity of wine grapes. Winemaking is also complicated by the formation of a protein haze in white wines and oxidative browning of red wines. We analyzed proteins in experimental Moravian white wines characterized by their instability and haze formation in bottles during storage despite prior bentonite treatment. Read More

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http://dx.doi.org/10.1002/bab.1736DOI Listing
February 2019
2 Reads

A novel strategy to synthesize dual-responsive polymeric nanocarriers for investigating the activity and stability of immobilized pectinase.

Biotechnol Appl Biochem 2019 Feb 4. Epub 2019 Feb 4.

Key Laboratory of Applied Surface and Colloid Chemistry, School of Chemistry & Chemical Engineering, Shaanxi Normal University, Xi'an, People's Republic of China.

A dual-stimuli-responsive support material for pectinase immobilization through ionic bonding was prepared. Specifically, polystyrene-b-polymethylacrylic (PS-b-PMAA), light- and pH-sensitive polystyrene-(5-propargylether-2-nitrobenzyl bromoisobutyrate)-b-poly(diethylamino)ethyl methacrylate-b-poly(polyethylene glycol methacrylate) (PS-ONB-PDEAEMA-b-PPEGMA) were synthesized through atom transfer radical polymerization, click chemistry, and hydrolysis. The two parts could self-assemble into the micelles in an aqueous solution. Read More

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http://dx.doi.org/10.1002/bab.1734DOI Listing
February 2019
2 Reads

High cell density culture of baker's yeast FX-2 based on pH-stat coupling with respiratory quotient.

Biotechnol Appl Biochem 2019 Feb 4. Epub 2019 Feb 4.

Angel Yeast (Yili) Co., Ltd., Yining, China.

The high cell density culture of baker's yeast FX-2 was investigated in a 50 L(A) automatic bioreactor. Herein, it was found firstly that the Crabtree effect clearly existed in batch fermentation with higher glucose content, then the critical initial glucose content range (≤2.00 g L ) was reasonably ascertained to effectively avoid Crabtree effect. Read More

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http://dx.doi.org/10.1002/bab.1735DOI Listing
February 2019
3 Reads

Enzyme production by solid-state fermentation on soybean meal: A comparative study of conventional and ultrasound-assisted extraction methods.

Biotechnol Appl Biochem 2019 Jan 29. Epub 2019 Jan 29.

Faculty of Technology and Metallurgy, University of Belgrade, Department of Biochemical Engineering and Biotechnology, Belgrade, Serbia.

Protease, cellulase, and α-amylase producing Bacillus subtilis strain was cultivated by solid-state fermentation technique using soybean meal as a substrate. The aim of the present study was to establish a highly efficient enzymes' extraction method as a first stage in downstream processing. The conventional extraction procedure was optimized by determining pH, stirring rate, solid/liquid ratio, and time of extraction on enzymes' recoveries from fermented soybean meal. Read More

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http://dx.doi.org/10.1002/bab.1732DOI Listing
January 2019

Novel microfluidic graphene oxide-protein amperometric biosensor for detecting sulfur compounds.

Biotechnol Appl Biochem 2019 Jan 22. Epub 2019 Jan 22.

Department of Life Science Engineering, Faculty of New Science and Technology, University of Tehran, Tehran, Iran.

Sulfur compounds are essential for many industries and organisms; however, they cause serious respiratory problems in human beings. Therefore, determination of sulfur concentration is of paramount importance. The research approach in the field of detecting contaminants has led to smaller systems that provide faster and more effective ways for diagnosis purposes. Read More

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http://dx.doi.org/10.1002/bab.1731DOI Listing
January 2019
2 Reads
1.362 Impact Factor

Production and characterization of PHB-HV copolymer by Bacillus thuringiensis isolated from Eisenia foetida.

Biotechnol Appl Biochem 2019 Jan 17. Epub 2019 Jan 17.

N. Rama Varier Ayurveda Foundation, Madurai, India.

Polyhydroxyalkanoates (PHAs) are one of the most important biopolyesters produced by vast number of microorganisms that exist in the environment. PHAs gained much attention due to its inherent biodegradable and biocompatible ability along with its physicochemical properties, which are similar to those of conventional petroleum-based plastics. In the present study, PHA producing bacterial strains was isolated from earthworm. Read More

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http://dx.doi.org/10.1002/bab.1730DOI Listing
January 2019
2 Reads

Modulation of callus growth and secondary metabolites in different Thymus species and Zataria multiflora micropropagated under ZnO nanoparticles stress.

Biotechnol Appl Biochem 2019 Jan 16. Epub 2019 Jan 16.

Department of Biological Sciences, National University of Medical Sciences, Rawalpindi, Pakistan.

Thymus species are aromatic plants with diverse applications in food industries and medicine. This study was conducted to evaluate the potential effect of ZnO nanoparticles (NPs) on callus proliferation and thymol and carvacrol production in three Thymus species, that is, T. vulgaris, T. Read More

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http://dx.doi.org/10.1002/bab.1727DOI Listing
January 2019
5 Reads

A novel screening method for potential naringinase-producing microorganisms.

Biotechnol Appl Biochem 2019 Jan 16. Epub 2019 Jan 16.

University Institute of Chemical Technology, Kavayitri Bahinabai Chaudhari North Maharashtra University, Jalgaon, Maharashtra, India.

Naringinase has high industrial importance, and the progress in naringinase research is still quite slow. The unavailability of an effective, simple screening method, which will be applicable to different microorganisms such as bacteria, fungi, and actinomycetes, is one of the main reasons for this gap. Therefore, a simple plate assay was developed for effective screening of microorganisms for naringinase by exposing to iodine vapors. Read More

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http://dx.doi.org/10.1002/bab.1728DOI Listing
January 2019
2 Reads

Comparative detection method of early onset cytokine-induced stress in β-cells (INS-1E).

Biotechnol Appl Biochem 2019 Jan 15. Epub 2019 Jan 15.

Department of Biochemistry and Microbiology, Nelson Mandela University, Port Elizabeth, Eastern Cape, South Africa.

β-Cells contain a prominent endoplasmic reticulum (ER), disrupting ER homeostasis and function, activating the unfolded protein response (UPR). Currently, no direct protocols measure the UPR initiation. Current methods to measure ER stress include the quantification of nitric oxide (NO) (indirect method), Western blotting, and qRT-PCR of downstream components. Read More

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http://doi.wiley.com/10.1002/bab.1729
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http://dx.doi.org/10.1002/bab.1729DOI Listing
January 2019
6 Reads

Matrilin-2 prevents the TNFα-induced apoptosis of WB-F344 cells via suppressing JNK pathway.

Biotechnol Appl Biochem 2019 Jan 9. Epub 2019 Jan 9.

Department of Pathology, Weihai Municipal Hospital, Weihai, 264200, Shandong Province, China.

Oval cells, a kind of hepatic progenitor cell quiescent at normal condition, activates to proliferate and differentiate into hepatocytes under severe and long-term liver injury, which usually raises severe inflammation. However, how oval cell survives in the inflammatory milieu interne is still unclear. Tumor necrosis factor α (TNFα), mimicking inflammatory hepatic milieu interne, was used to treat oval cell line, WB-F344, to test the protective function of matrilin-2. Read More

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http://dx.doi.org/10.1002/bab.1726DOI Listing
January 2019
2 Reads
1.362 Impact Factor

The role of gibberellin synthase gene GhGA2ox1 in upland cotton (Gossypium hirsutum L.) responses to drought and salt stress.

Biotechnol Appl Biochem 2019 Jan 8. Epub 2019 Jan 8.

State Key Laboratory of Cotton Biology, Institute of Cotton Research of Chinese Academy of Agricultural Sciences, Anyang, People's Republic of China.

Gibberellins (GAs) is one kind of important endogenous hormone in plants that regulates vegetative and reproductive growth of plants. GA2ox is a class of oxidase that plays a regulatory role in the third stage of GAs synthesis. In this paper, we cloned the GhGA2ox1 gene from an upland cotton (Gossypium hirsutum L. Read More

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http://doi.wiley.com/10.1002/bab.1725
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http://dx.doi.org/10.1002/bab.1725DOI Listing
January 2019
2 Reads

Preparation and anti-tumor efficiency evaluation of bacterial magnetosome-anti-4-1BB antibody complex: Bacterial magnetosome as antibody carriers isolated from Magnetospirillum gryphiswaldense.

Biotechnol Appl Biochem 2019 Jan 1. Epub 2019 Jan 1.

Department of Immunology, Cancer Institute and Cancer Hospital, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing, People's Republic of China.

Bacterial magnetosomes (BMs) are used as carriers for antibodies, enzymes, and nucleic acids. This study aimed to demonstrate the clinical utility of BMs derived from Magnetospirillum gryphiswaldense for use in anti-tumor immunotherapy. Bis(sulfosuccinimidyl) suberate (BS3) was used to prepare BM-anti-4-1BB antibody complexes. Read More

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http://dx.doi.org/10.1002/bab.1724DOI Listing
January 2019
1 Read

Production of a novel N-terminal PEGylated crisantaspase.

Biotechnol Appl Biochem 2018 Dec 29. Epub 2018 Dec 29.

Department of Biochemical and Pharmaceutical Technology, University of São Paulo, São Paulo, Brazil.

Crisantaspase is an asparaginase enzyme produced by Erwinia chrysanthemi and used to treat acute lymphoblastic leukemia (ALL) in case of hypersensitivity to Escherichia coli l-asparaginase (ASNase). The main disadvantages of crisantaspase are the short half-life (10 H) and immunogenicity. In this sense, its PEGylated form (PEG-crisantaspase) could not only reduce immunogenicity but also improve plasma half-life. Read More

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http://dx.doi.org/10.1002/bab.1723DOI Listing
December 2018
5 Reads

Cytoprotective, anti-inflammatory, and antioxidant properties of high-molecular-weight hyaluronan enriched with red orange extract in human fibroblasts exposed to ultra violet light B irradiation.

Biotechnol Appl Biochem 2018 Dec 27. Epub 2018 Dec 27.

PharmaChemistry Line, Istituto Italiano di Tecnologia, Genova, Italy.

Ultraviolet (UV) light exposure is the primary factor responsible for skin photoaging, affecting all the skin layers, mainly through the production of reactive oxygen species (ROS), activation of inflammatory responses, and apoptosis. In keeping with this evidence, exogenous supplementation with dietary antioxidants has been shown to provide photoprotective benefits. Moreover, oral administration of hyaluronic acid (HA) has been proved to reduce the signs of aged skin, such as wrinkles, and increase hydration and elasticity. Read More

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http://doi.wiley.com/10.1002/bab.1722
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http://dx.doi.org/10.1002/bab.1722DOI Listing
December 2018
20 Reads

Isolation and screening for limonin-producing endophytic bacteria from Citrus maxima (Burm.) Merr. cv. Shatian Yu.

Biotechnol Appl Biochem 2019 Mar 9;66(2):192-201. Epub 2019 Jan 9.

College of Life Science, Shanxi University, Taiyuan, China.

Limonin, a compound of highly oxidized triterpenoids, has potential functions in preventing or slowing the occurrences of many diseases. In this study, five different bacterial strains were isolated and identified from Citrus maxima (Burm.) Merr. Read More

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http://doi.wiley.com/10.1002/bab.1721
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http://dx.doi.org/10.1002/bab.1721DOI Listing
March 2019
3 Reads

Bioconversion of lignin and its derivatives into polyhydroxyalkanoates: Challenges and opportunities.

Biotechnol Appl Biochem 2019 Mar 28;66(2):153-162. Epub 2018 Dec 28.

Department of Chemical Engineering, Chungbuk National University, Chungbuk, Republic of Korea.

Renewable energy resources are considered to be promising for the development of a sustainable circular economy. Among various alternatives, the microbial route for various biofuels production is quite lucrative. Use of cellulose and lignocellulose for methane, H , organic acids, ethanol, and cellulase has been explored a lot in the past few decades. Read More

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http://dx.doi.org/10.1002/bab.1720DOI Listing
March 2019
2 Reads
1.362 Impact Factor

Salidroside protected against MPP -induced Parkinson's disease in PC12 cells by inhibiting inflammation, oxidative stress and cell apoptosis.

Biotechnol Appl Biochem 2019 Mar 24;66(2):247-253. Epub 2018 Dec 24.

Department of Neurosurgery, First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China.

The present study aimed to investigate the protective effects of salidroside (SAL) on 1-methyl-4-phenylpyridinium (MPP )-induced PC12 cell model for Parkinson's disease. PC12 cells were pretreated with SAL in different concentrations and then exposed to MPP . To evaluate the effects of SAL on cytotoxicity, the survival rate was tested by the 3-(4,5-dimethylthiazol-2-yl)-2,5-dimethyltetrazolium bromide (MTT) assay and the apoptosis was tested via flow cytometry and Western blot. Read More

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http://dx.doi.org/10.1002/bab.1719DOI Listing
March 2019
12 Reads

Employment of fucosidases for the synthesis of fucosylated oligosaccharides with biological potential.

Biotechnol Appl Biochem 2019 Mar 3;66(2):172-191. Epub 2018 Dec 3.

Departamento de Biotecnología, Universidad Autónoma Metropolitana-Iztapalapa, Ciudad de México, México.

Fucosylated oligosaccharides play important physiological roles in humans, including in the immune response, transduction of signals, early embryogenesis and development, growth regulation, apoptosis, pathogen adhesion, and so on. Efforts have been made to synthesize fucosylated oligosaccharides, as it is difficult to purify them from their natural sources, such as human milk, epithelial tissue, blood, and so on. Within the strategies for its in vitro synthesis, it is remarkable the employment of fucosidases, enzymes that normally cleave the fucosyl residue from the non-reducing end of fucosylated compounds, as these enzymes are also capable of synthesizing them by means of a transfucosylation reaction. Read More

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http://dx.doi.org/10.1002/bab.1714DOI Listing
March 2019
2 Reads

SOX11 regulates apoptosis and cell cycle in hepatocellular carcinoma via Wnt/β-catenin signaling pathway.

Biotechnol Appl Biochem 2019 Mar 17;66(2):240-246. Epub 2018 Dec 17.

Department of Hepatobiliary Surgery, Affiliated Hospital of North Sichuan Medical College, Nanchong, People's Republic of China.

Hepatocellular carcinoma (HCC) is the most common type of liver cancer with high mortality. Identifying key molecules involved in the regulation of HCC development is of great clinical significance. SOX11 is a transcription factor belonging to group C of Sry-related high mobility group box family whose abnormal expression is frequently seen in many kinds of human cancers. Read More

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http://doi.wiley.com/10.1002/bab.1718
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http://dx.doi.org/10.1002/bab.1718DOI Listing
March 2019
9 Reads

Enhanced cecropin B2 production via chitin-binding domain and intein self-cleavage system.

Biotechnol Appl Biochem 2019 Mar 4;66(2):209-215. Epub 2018 Dec 4.

Department of Chemical Engineering, National Chung Hsing University, Taichung, Taiwan, Republic of China.

In this study, various constructs and hosts were used to produce high levels of cecropin B2 (cecB2). To mitigate cecB2's toxic inhibition of host cells, various cecB2 constructs were built. Results showed that the combination of a chitin-binding domain and an intein self-cleavage motif in front of cecropin B2, without a His-tag, was best for cecB2 expression. Read More

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http://dx.doi.org/10.1002/bab.1716DOI Listing
March 2019
15 Reads

Cai's Neiyi Prescription promotes apoptosis and inhibits inflammation in endometrial stromal cells with endometriosis through inhibiting USP10.

Biotechnol Appl Biochem 2019 Mar 4;66(2):231-239. Epub 2018 Dec 4.

Department of Traditional Chinese Medicine, Shanghai General Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, People's Republic of China.

To observe the effect of Cai's Neiyi Prescription (CNYP) on the apoptosis and inflammation in endometrial stromal cells with endometriosis (EM) both in vivo and in vitro, EM model rats and endometrial stromal cells were treated with CNYP and the level of USP10, p-ERK1/2, ERK1/2, and apoptosis-related protein as well as the levels of proinflammatory factors were measured by Western blotting and ELISA, respectively. Rats with surgically induced EM showed increased USP10 expression and ERK/2 activation. Intragastric administration of CNYP granule significantly inhibited EM-induced ERK1/2 activation and expression of USP10 and Bcl-2, but increased the expression of Bax and Caspase-7 in EM-induced rats. Read More

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http://dx.doi.org/10.1002/bab.1715DOI Listing
March 2019
7 Reads

Effective filter screening approach with fractionation for enabling inline filtration of protein A eluate.

Biotechnol Appl Biochem 2019 Mar 30;66(2):216-223. Epub 2018 Nov 30.

Purification Process Sciences, MedImmune LLC, One MedImmune Way, Gaithersburg, MD, USA.

Filtration of protein A eluates inline with a chromatography column is a common challenge for monoclonal antibody (mAb) purification due to the high system backpressure during elution, which can result in system shut down or require a decreased elution flow rate. The inability to filter inline not only poses a risk for process deviations, but can also lead to tank constraints and microbial ingress risk. Here, we evaluated and described a novel approach for identifying filters for inline filtration of protein A eluates at pilot scale. Read More

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http://dx.doi.org/10.1002/bab.1713DOI Listing
March 2019
2 Reads
1.362 Impact Factor

Application of ultra-rapid qPCR and DNA chips for viral RNA detection and confirmation.

Biotechnol Appl Biochem 2019 Mar 5;66(2):224-230. Epub 2018 Dec 5.

Department of Life Science, Kyonggi University, Suwon, Republic of Korea.

The rapid and accurate detection of the presence of microorganisms, such as viruses, has been an important issue in the fields of public health, as well as agriculture. A PCR-based detection method has been developed and applied in these fields to determine the presence of specific pathogens. Although the major advantage of real-time PCR is the monitoring of amplification and ability to quantify the template genes, the method described here should solve the problem of nonspecific product synthesis. Read More

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http://doi.wiley.com/10.1002/bab.1711
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http://dx.doi.org/10.1002/bab.1711DOI Listing
March 2019
9 Reads

Effects of multicopper oxidase orientation in multiwalled carbon nanotube biocathodes on direct electron transfer.

Biotechnol Appl Biochem 2019 Mar 23;66(2):137-141. Epub 2018 Nov 23.

Department of Frontier Fiber Technology and Science, Graduate School of Engineering, University of Fukui, Fukui, Japan.

In this study, multicopper oxidase (MCO) was immobilized on multiwalled carbon nanotubes (MWCNTs) at two different orientations, and the electrochemical properties of the resulting cathodes were investigated. Using N- or C-terminal His-tagged MCO and MWCNTs, we constructed two types of cathodes. We assumed that the distance between the type 1 (T1)Cu of the C-terminal His-tagged MCO and the MWCNT surface was lesser than that between the T1Cu of the N-terminal His-tagged MCO and the MWCNT surface. Read More

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http://doi.wiley.com/10.1002/bab.1710
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http://dx.doi.org/10.1002/bab.1710DOI Listing
March 2019
3 Reads

Identification of the first Crocodylus siamensis cathelicidin gene and RN15 peptide derived from cathelin domain exhibiting antibacterial activity.

Biotechnol Appl Biochem 2019 Mar 23;66(2):142-152. Epub 2018 Nov 23.

Protein and Proteomics Research Center for Commercial and Industrial Purposes (ProCCI), Faculty of Science, Khon Kaen University, Khon Kaen, Thailand.

Cathelicidins are effector molecules of vertebrate immunity that play vital roles against microbial invasion. They are widely identified in mammals, but few have been reported in Crocodilians, which are considered to be species with a powerful immune system. In the present study, we identified and characterized a novel cathelicidin from the blood of the Siamese crocodile, Crocodylus siamensis. Read More

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http://doi.wiley.com/10.1002/bab.1709
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http://dx.doi.org/10.1002/bab.1709DOI Listing
March 2019
10 Reads

Circulating miR-26a and miR-21 as biomarkers for glioblastoma multiform.

Biotechnol Appl Biochem 2019 Mar 19;66(2):261-265. Epub 2018 Nov 19.

Department of Biochemistry, Pasteur Institute of Iran, Tehran, Iran.

Glioblastoma multiform is the most common and lethal primary central nervous system tumor. Circulating microRNAs (miRNAs), present in cell-free bodily fluids, have been gaining importance as cancer biomarkers. The primary aim of this study was to assess whether circulating miRNA-128, -21, and -26a in glioblastoma patients can be used as diagnostic biomarkers. Read More

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http://doi.wiley.com/10.1002/bab.1707
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http://dx.doi.org/10.1002/bab.1707DOI Listing
March 2019
22 Reads

The C-terminal region of tumor necrosis factor like weak inducer of apoptosis is required for interaction with advanced glycation end products.

Biotechnol Appl Biochem 2019 Mar 20;66(2):254-260. Epub 2018 Dec 20.

Department of Pharmacology, School of Pharmacy, Shujitsu University, Okayama, Japan.

Previously, we found that endogenously produced pro-inflammatory molecules, advanced glycation end products (AGEs), interact with tumor necrosis factor-like weak inducer of apoptosis (TWEAK), and attenuate its immunomodulatory function. In the present study, to elucidate the mechanism by which AGEs attenuate TWEAK function, we searched for regions responsible for TWEAK-AGE interaction using TWEAK deletion mutants. Pull-down assays with the TWEAK mutants and AGEs revealed that the C-terminal half of TWEAK, which is the region essential for receptor stimulation, was required for this interaction. Read More

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http://dx.doi.org/10.1002/bab.1706DOI Listing
March 2019
15 Reads

Antioxidant and reactive oxygen species scavenging properties of cellular albumin in HepG2 cells is mediated by the glutathione redox system.

Biotechnol Appl Biochem 2019 Mar 23;66(2):163-171. Epub 2018 Nov 23.

Department of Hematology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.

This study was carried out to examine the role of intracellular albumin in the modulation of oxidative damage induced by glutathione modifiers in HepG2 cells. Also, the relationship of albumin synthesis with oxidative stress factors including antioxidants was studied. HepG2 cell culture was supplemented with glutathione modifiers; L-Buthionine-sulfoximine (BSO; 0. Read More

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http://dx.doi.org/10.1002/bab.1708DOI Listing
March 2019
3 Reads

Expression of the hybrid antimicrobial peptide lactoferrin-lysozyme in Pichia pastoris.

Biotechnol Appl Biochem 2019 Mar 27;66(2):202-208. Epub 2018 Nov 27.

Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, People's Republic of China.

The use of lactoferrin antimicrobial peptides and lysozymes as traditional antibiotic alternatives is suitable for solving drug residue and pathogen resistance. In this study, bovine lactoferrin (LfcinB) and human lysozyme (hLY) were combined through fusion expression in Pichia pastoris GS115 driven by constitutive GAP promoter. For neutralizing the toxic property of the antimicrobial peptide, anion antioxidant peptides from porcine myofibrillar protein and enzymatically hydrolyzed chicken egg white were fused to the hybrid antimicrobial peptide LfcinB-hLY. Read More

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http://dx.doi.org/10.1002/bab.1705DOI Listing
March 2019
3 Reads

Expression and production of recombinant scorpine as a potassium channel blocker protein in Escherichia coli.

Biotechnol Appl Biochem 2019 Jan 12;66(1):119-129. Epub 2018 Nov 12.

Department of Biotechnology, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Scorpine is a cationic protein from the venom of Pandinus imperator, belonging to potassium channel blocker family, which has been shown to have antibacterial, antiviral, and antiplasmodia activities. In the present study, a pET-44a vector containing scorpine synthetic gene with T7 Promoter (pET 44a-His6-Nus-His6-tev-scorpine) was transferred into Escherichia coli Rosetta-gami B (DE3) for soluble expression of the protein in the cytoplasm and its overproduction. After confirming recombinant scorpine peptide expression using SDS-PAGE and Western blot, augmentation of production was performed during two stages. Read More

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http://doi.wiley.com/10.1002/bab.1704
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http://dx.doi.org/10.1002/bab.1704DOI Listing
January 2019
18 Reads
1.362 Impact Factor

Production of high crystallinity type-I cellulose from Komagataeibacter hansenii JR-02 isolated from Kombucha tea.

Biotechnol Appl Biochem 2019 Jan 29;66(1):108-118. Epub 2018 Nov 29.

State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi University, Nanning, Guangxi, People's Republic of China.

In this study, a bacterial cellulose (BC) producing strain was isolated from Kombucha tea and identified as Komagataeibacter hansenii JR-02 by morphological, physiological, and biochemical characterization and 16S rRNA sequence. Then, the media components and culture conditions for BC production were optimized. Result showed that the highest BC yield was 3. Read More

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http://doi.wiley.com/10.1002/bab.1703
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http://dx.doi.org/10.1002/bab.1703DOI Listing
January 2019
3 Reads

Improved activity of α-L-arabinofuranosidase from Geobacillus vulcani GS90 by directed evolution: Investigation on thermal and alkaline stability.

Biotechnol Appl Biochem 2019 Jan 8;66(1):101-107. Epub 2018 Nov 8.

Department of Chemistry, İzmir Institute of Technology, İzmir, Turkey.

α-L-Arabinofuranosidase (Abf) is a potential enzyme because of its synergistic effect with other hemicellulases in agro-industrial field. In this study, directed evolution was applied to Abf from Geobacillus vulcani GS90 (GvAbf) using one round error-prone PCR and constructed a library of 73 enzyme variants of GvAbf. The activity screening of the enzyme variants was performed on soluble protein extracts using p-nitrophenyl α-L-arabinofuranoside as substrate. Read More

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http://dx.doi.org/10.1002/bab.1702DOI Listing
January 2019
1 Read