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    2036 results match your criteria Biotechnology and Applied Biochemistry[Journal]

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    Structure-based analysis of Clot as a thioredoxin-related protein of 14 kDa in Drosophila via experimental and computational approaches.
    Biotechnol Appl Biochem 2017 Oct 28. Epub 2017 Oct 28.
    Department of Medical Biotechnology, Soonchunhyang University, Asan, 31538, Korea.
    The clot gene is required for the biosynthesis of drosopterins, the red components of Drosophila eye pigments. However, the enzymatic role of Clot in Drosophila eye pigment formation and the molecular mechanisms underlying Clot function are not fully elucidated. In this study, we cloned and characterized Clot derived from Drosophila cDNA, and results showed that Clot exhibited ∼30% sequence identity with mammalian TRP14. Read More

    Large-scale purification of high purity α1-antitrypsin from Cohn Fraction IV with virus inactivation by solvent/detergent and dry-heat treatment.
    Biotechnol Appl Biochem 2017 Oct 26. Epub 2017 Oct 26.
    Beijing Key Laboratory of Blood Safety and Supply Technologies, Beijing Institute of Transfusion Medicine, Beijing, 100850, China.
    α1-antitrypsin (AAT) is widely used to treat patients with congenital AAT deficiency. Cohn Fraction IV (Cohn F IV) is normally discarded during the manufacturing process of albumin but contains approximately 33% of plasma AAT. We established a new process for large-scale purification of AAT from it. Read More

    Impact of mycolic acid deficiency on cells of Corynebacterium glutamicum ATCC13869.
    Biotechnol Appl Biochem 2017 Oct 26. Epub 2017 Oct 26.
    School of Biotechnology, Jiangnan University, Wuxi, People's Republic of China.
    Mycolic acid (MA) plays important role in Corynebacterium glutamicum, but the key enzymes in the biosynthetic pathway of MA in C. glutamicum ATCC13869 have not been characterized. Since the locus BBD29_RS14045 in C. Read More

    Trans-acting oligodeoxythymidine phosphorothioate triester reagents for transient transfection optimized and facilitated by a high-throughput microbioreactor system.
    Biotechnol Appl Biochem 2017 Oct 10. Epub 2017 Oct 10.
    Division of Biotechnology Review and Research II, OBP, CDER, FDA, Silver Spring, MD.
    A rapid and cost-effective transient transfection method for mammalian cells is essential for screening biopharmaceuticals in early stages of development. A library of 25 amphipathic trans-acting oligodeoxythymidine phosphorothioate triester (dTtaPS) transfection reagents, carrying positively charged and lipophilic groups, has been constructed for this purpose. High-throughput screening of the library, using an imaging cytometer and an automated microbioreactor system, has led to the identification of dTtaPS(10+) as a potent transfection reagent. Read More

    Biosensors: Classifications, medical applications and future prospective.
    Biotechnol Appl Biochem 2017 Oct 10. Epub 2017 Oct 10.
    Faculty of Applied Medical Sciences, Department of Medical Laboratory Technology, King Abdulaziz University, P. O. Box 80402, Jeddah, 21589, Kingdom of Saudi Arabia.
    Biosensors are devices that combine a biological material with a suitable platform for detection of pathogenic organisms, carcinogenic, mutagenic and/or toxic chemicals or for reporting a biological effect. In recent years, enormous number of different types of biosensors have been constructed and developed for several medical applications. The reason for that was primarily due to the numerous advantages and applications that can be offered by biosensors. Read More

    The BMP antagonist Noggin is produced by osteoblasts in response to the presence of prostate cancer cells.
    Biotechnol Appl Biochem 2017 Oct 5. Epub 2017 Oct 5.
    Department of Oncology and Metabolism, Medical School, University of Sheffield, Sheffield, United Kingdom.
    Bone metastasis is a key event responsible for morbidity in prostate cancer patients. Interactions between prostate cancer cells and the bone microenvironment facilitate survival of tumor cells and alter bone turnover, a process that is thought to enhance the growth of metastases in this site. This study aimed to test the hypothesis that the presence of tumors cells increases transforming growth factor beta (TGF-β) signaling in bone and that this regulates the proliferation and differentiation of osteoblastic lineage cells in metastatic sites. Read More

    Construction of tissue-engineered lymphatic vessel using human adipose derived stem cells differentiated lymphatic endothelial like cells and decellularized arterial scaffold: A preliminary study.
    Biotechnol Appl Biochem 2017 Oct 5. Epub 2017 Oct 5.
    Department of Microsurgery and Orthopedic Trauma, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, People's Republic of China.
    We have previously demonstrated that human adipose-derived stem cells (hADSCs) can be differentiated into lymphatic endothelial like cells. The purpose of this study was to investigate the feasibility of utilizing the induced lymphatic endothelial like cells and decellularized arterial scaffold to construct the tissue-engineered lymphatic vessel. The hADSCs were isolated from adipose tissue in healthy adults and were characterized the multilineage differentiation potential. Read More

    Progress in biopharmaceutical development.
    Biotechnol Appl Biochem 2017 Oct 3. Epub 2017 Oct 3.
    Department of Bioengineering, Institute of Biotechnology and Antibiotics, Warsaw, Poland.
    Since its introduction in 1982, biopharmaceutical drugs have revolutionized the treatment of a broad spectrum of diseases and are increasingly used in nearly all branches of medicine. In recent years, the biopharmaceuticals market has developed much faster than the market for all drugs and is believed to have great potential for further dynamic growth because of the tremendous demand for these drugs. Biobetters, which contain altered active pharmaceutical ingredients with enhanced efficacy, will play an important role in the development of biopharmaceuticals. Read More

    FleQ regulates both the type VI secretion system and flagella in Pseudomonas putida.
    Biotechnol Appl Biochem 2017 Oct 2. Epub 2017 Oct 2.
    State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, People's Republic of China.
    Based on the analysis of sodium dodecyl sulfate-polyacrylamide gel electrophoresis and mass spectrometry, flagellin FliC and a key member of the type VI secretion system Hcp1 were found to be secreted from Pseudomonas putida KT2442 cells in the late exponential phase, but in the early exponential phase only FliC was secreted. Relevant genes fleQ and rpoN were then individually deleted in P. putida KT2442, and the expression and transcription of fliC and hcp1 in these mutant strains were analyzed. Read More

    Studying the TRAF2 binding to model membranes: The role of subunits dissociation.
    Biotechnol Appl Biochem 2017 Sep 28. Epub 2017 Sep 28.
    Department of Experimental Medicine and Surgery, University of Rome Tor Vergata, Via Montpellier 1, 00133, Rome, Italy.
    The ability of a C-terminal truncated form of TRAF2 to bind synthetic vesicles has been quantitatively studied by steady-state fluorescence energy transfer from the protein to large unilamellar vesicles (LUVs) prepared with different lipid mixtures. The dissociation constants, the free energy of binding and the average number of phospholipids interacting with truncated-TRAF2 have been evaluated from the corresponding binding curves. The results indicate that the protein strongly interacts with the lipid bilayer, preferentially in the monomeric state. Read More

    Amine oxidase from Euphorbia characias: Kinetic and structural characterization.
    Biotechnol Appl Biochem 2017 Sep 20. Epub 2017 Sep 20.
    Department of Sciences of Life and Environment, University of Cagliari, Monserrato, Cagliari, Italy.
    This minireview focuses on a plant copper/2,4,5-trihydroxyphenyl alanine quinone amine oxidase isolated from the latex of the shrub Euphorbia characias (ELAO). This enzyme has been investigated in terms of both molecular structure and kinetic mechanism. The characterization of this enzyme allowed us to identify specific amino acids and domains that play a key role in modulating substrate access into the active site not only for ELAO but also for other plant and mammalian amine oxidases. Read More

    Working at the membrane interface: Ligand-induced changes in dynamic conformation and oligomeric structure in human aromatase.
    Biotechnol Appl Biochem 2017 Sep 19. Epub 2017 Sep 19.
    Department of Life Sciences and Systems Biology, University of Torino, Torino, Italy.
    Aromatase catalyzes the biosynthesis of estrogens from androgens. Owing to the physiological importance of this conversion of lipophilic substrates, the interaction with the lipid bilayer for this cytochrome P450 is crucial for its dynamics that must allow an easy access to substrates and inhibitors. Here, the aromatase-anastrozole interaction is studied by combining computational methods to identify possible access/egress routes with the protein inserted in the membrane and experimental tools aimed at the investigation of the effect of the inhibitor on the protein conformation. Read More

    Construction and application of an electrochemical biosensor based on an endotoxin aptamer.
    Biotechnol Appl Biochem 2017 Sep 9. Epub 2017 Sep 9.
    College of Pharmaceutical Science, ZheJiang University, HangZhou, People's Republic of China.
    An electrochemical biosensor that used an aptamer as a biological element was constructed to detect endotoxin. Biolayer interferometry was used to obtain the affinity constant of an aptamer for lipopolysaccharide, which had an equilibrium dissociation constant of 22.9 nM. Read More

    Improving properties of silk sericin membranes via enzymatic oxidation with laccase and TEMPO.
    Biotechnol Appl Biochem 2017 Sep 7. Epub 2017 Sep 7.
    Key Laboratory of Science and Technology of Eco-Textile, Ministry of Education, Jiangnan University, Wuxi, People's Republic of China.
    Silk sericin has excellent features of antioxidant ability and good cytocompatibility; however, high water solubility and poor mechanical properties have restricted its application in biomedical fields. In this study, aimed at improving the mechanical properties of a regenerated silk sericin, the primary hydroxy groups in silk sericin were enzymatically oxidized by using laccase and 2,2',6,6'-tetramethylpiperidine-N-oxyl (TEMPO), and the generated reactive groups then reacted further with the amino groups in the sericin chains. The efficacy of the enzymatic cross-linking was evaluated by means of determination of amino groups, SDS polyacrylamide gel electrophoresis, Fourier transform infrared (FTIR) spectroscopy, and size exclusion chromatography. Read More

    An activity transition from NADH dehydrogenase to NADH oxidase during protein denaturation.
    Biotechnol Appl Biochem 2017 Sep 7. Epub 2017 Sep 7.
    Department of Chemistry, Rutgers University-Camden, Camden, NJ, USA.
    A decrease in the specific activity of an enzyme is commonly observed when the enzyme is inappropriately handled or is stored over an extended period. Here, we reported a functional transition of an FMN-bound diaphorase (FMN-DI) that happened during the long-term storage process. It was found that FMN-DI did not simply lose its β-nicotinamide adenine diphosphate (NADH) dehydrogenase activity after a long-time storage, but obtained a new enzyme activity of NADH oxidase. Read More

    Membrane cholesterol effect on the 5-HT2A receptor: Insights into the lipid-induced modulation of an antipsychotic drug target.
    Biotechnol Appl Biochem 2017 Sep 6. Epub 2017 Sep 6.
    Research Programme on Biomedical Informatics (GRIB), Department of Experimental and Health Sciences of Pompeu Fabra University (UPF)-Hospital del Mar Medical Research Institute (IMIM), Barcelona, Spain.
    The serotonin 5-hydroxytryptamine 2A (5-HT2A ) receptor is a G-protein-coupled receptor (GPCR) relevant for the treatment of CNS disorders. In this regard, neuronal membrane composition in the brain plays a crucial role in the modulation of the receptor functioning. Since cholesterol is an essential component of neuronal membranes, we have studied its effect on the 5-HT2A receptor dynamics through all-atom MD simulations. Read More

    Taxadiene-5α-ol is a minor product of CYP725A4 when expressed in Escherichia coli.
    Biotechnol Appl Biochem 2017 Sep 6. Epub 2017 Sep 6.
    Department of Plant Biology, Southern Illinois University, Carbondale, IL, USA.
    CYP725A4 is a P450 enzyme from Taxus cuspidata that catalyzes the formation of taxadiene-5α-ol (T5α-ol) from taxadiene in paclitaxel biosynthesis. Past attempts expressing CYP725A4 in heterologous hosts reported the formation of 5(12)-oxa-3(11)-cyclotaxane (OCT) and/or 5(11)-oxa-3(11)-cyclotaxane (iso-OCT) instead of, or in addition to, T5α-ol. Here, we report that T5α-ol is produced as a minor product by Escherichia coli expressing both taxadiene synthase and CYP725A4. Read More

    Enhancement of  l-phenylalanine production in Escherichia coli by heterologous expression of Vitreoscilla hemoglobin.
    Biotechnol Appl Biochem 2017 Sep 5. Epub 2017 Sep 5.
    Engineering Research Center of Industrial Microbiology, Ministry of Education, Fujian Normal University, Fuzhou, People's Republic of China.
    l-Phenylalanine is an important amino acid that is widely used in the production of food flavors and pharmaceuticals. Generally, l-phenylalanine production by engineered Escherichia coli requires a high rate of oxygen supply. However, the coexpression of Vitreoscilla hemoglobin gene (vgb), driven bya tac promoter, with the genes encoding 3-deoxy-d-arabinoheptulosonate-7-phosphate synthetase (aroF) and feedback-resistant chorismate mutase/prephenate dehydratase (pheA(fbr) ), led to increased productivity and decreased demand for aeration by E. Read More

    Size of single-wall carbon nanotube affects the folate receptor-mediated cancer cell targeting.
    Biotechnol Appl Biochem 2017 Aug 30. Epub 2017 Aug 30.
    Department of Nanobiotechnology, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran.
    Advances in nanobiotechnology and targeting strategy could improve the delivery of therapeutic molecules into cancer cells, leading to improved treatment efficiency with minimal side effects on normal cells. To design an efficient nanocarrier, consideration of parameters that facilitate direct drug delivery into the target cells is important. We studied the effect of single-wall carbon nanotubes (SWNTs) size on their cell internalization level via the folate receptor-mediated pathway through folic acid targeting. Read More

    A camelid nanobody against EGFR was easily obtained through refolding of inclusion body expressed in Escherichia coli.
    Biotechnol Appl Biochem 2016 Nov 7. Epub 2016 Nov 7.
    School of Life Science and Biotechnology, Dalian University of Technology, Dalian City, Liaoning Province, People's Republic of China.
    Using anti-EGFR (epidermal growth factor receptor) nanobody is a good choice for diagnoses and therapeutics for high EGFR expression diseases. In the present study, the percentage composition of anti-EGFR nanobody attained 25% of the total cell protein expressed in Escherichia coli BL21 (DE3). However, almost all nanobodies were expressed as inclusion bodies. Read More

    Enhancing recombinant interleukin-6 production yield by fermentation optimization, two-step denaturing, and one-step purification.
    Biotechnol Appl Biochem 2017 Aug 22. Epub 2017 Aug 22.
    National Centre of Excellence in Molecular Biology, University of the Punjab, Lahore, Pakistan.
    Interleukin-6 a pleiotropic cytokine involved in a wide range of biological activities. So the large-scale production of biologically active recombinant human interleukin-6 is important for its structural and functional studies. Here, we report an optimized method for shake flask fermentation and a simplified high-yield purification procedure for the recombinant interleukin-6. Read More

    Generation and selection of naïve Fab library for parasitic antigen: Anti-BmSXP antibodies for lymphatic filariasis.
    Biotechnol Appl Biochem 2017 Aug 21. Epub 2017 Aug 21.
    Institute for Research in Molecular Medicine, Universiti Sains Malaysia, Minden, Penang, Malaysia.
    Phage display has been applied successfully as a tool for the generation of monoclonal antibodies (mAbs). Naive antibody libraries are unique as they are able to overcome several limitations associated with conventional mAb generation methods like the hybridoma technology. Here, we performed an in vitro selection and generation of Fab antibodies against Brugia malayi SXP protein (BmSXP), a recombinant antigen for the detection of lymphatic filariasis. Read More

    Microbial production of mannitol by Lactobacillus brevis 3-A5 from concentrated extract of Jerusalem artichoke tubers.
    Biotechnol Appl Biochem 2017 Aug 17. Epub 2017 Aug 17.
    Dalian Institute of Chemical Physics, Chinese Academy of Sciences, CAS, Dalian, 116023, PR China.
    In the present study, the conversion of the extract of Jerusalem artichoke tubers for mannitol production by Lactobacillus brevis 3-A5 was investigated. When the bacterium utilized enzymatic hydrolysates of Jerusalem artichoke extract as the main substrates in batch fermentation, the significant decrease in mannitol productivity was observed when the initial concentration of reducing sugar increased. Then, a strategy of continuous fed-batch fermentation was adopted for improving mannitol production with enzymatic hydrolysates of Jerusalem artichoke extract as main substrates. Read More

    In silico mapping of allosteric ligand binding sites in type-1 cannabinoid receptor.
    Biotechnol Appl Biochem 2017 Aug 17. Epub 2017 Aug 17.
    European Center for Brain Research (CERC)/Santa Lucia Foundation IRCCS, Rome, Italy.
    The recent resolution of the crystal structure of type-1 cannabinoid receptor (CB1 ), and the discovery of novel modulators for this target open the way to the possibility of elucidating the structural requirements for CB1 binding, and thereby facilitate a rational drug design. Compounds that target the orthosteric site of CB1 in some cases have shown side effects. Allosteric modulators could potentially avoid these side effects by influencing binding and/or efficacy of orthosteric ligands. Read More

    Cloning and bacterial expression systems for recombinant human heparanase production: Substrate specificity investigation by docking of a putative heparanase substrate.
    Biotechnol Appl Biochem 2017 Aug 14. Epub 2017 Aug 14.
    Institute of Food Science, CNR, Avellino, Italy.
    Human heparanase (HPSE) is an enzyme that degrades the extracellular matrix. It is implicated in a multiplicity of physiological and pathological processes encouraging angiogenesis and tumor metastasis. The protein is a heterodimer composed of a subunit of 8 kDa and another of 50 kDa. Read More

    Prediction of shorter oligonucleotide sequences recognizing aflatoxin M1.
    Biotechnol Appl Biochem 2017 Aug 10. Epub 2017 Aug 10.
    Dairy Chemistry Division, National Dairy Research Institute, Karnal, Haryana, India.
    Aflatoxin M1 (AFM1) is present in milk of lactating animals fed on aflatoxin B1 contaminated feeds. Aptamers are new emerging ligand molecules and can be employed in assay protocols. Shorter aptamers have several advantages. Read More

    Effects of gelling bath on the physical properties of alginate gel beads and the biological characteristics of entrapped HepG2 cells.
    Biotechnol Appl Biochem 2017 Aug 9. Epub 2017 Aug 9.
    Scientific Research Center for Translational Medicine, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, People's Republic of China.
    Optimizing alginate gel beads is necessary to support the survival, proliferation, and function of entrapped hepatocytes. In this study, gelling bath was modified by decreasing calcium ion concentration and increasing sodium ion concentration. Alginate gel beads (using 36% G sodium alginate) prepared in the modified gelling bath had more homogeneous structure and better mass transfer properties compared with the traditional gelling bath that contains only calcium ions. Read More

    Molecular dynamics study on the Apo- and Holo-forms of 5-lipoxygenase.
    Biotechnol Appl Biochem 2017 Aug 8. Epub 2017 Aug 8.
    European Center for Brain Research (CERC)/Santa Lucia Foundation IRCCS, Rome, Italy.
    Lipoxygenases (LOXs) are nonheme iron-containing enzymes catalyzing the dioxygenation of polyunsaturated fatty acids. LOX catalytic activity depends on the presence of iron in the active site and the iron removal is also able to affect the membrane binding properties of the enzyme. Leukotrienes biosynthesis is initiated by the action of 5-LOX at the level of nuclear membrane and the mechanism of enzyme-membrane interaction is thought to involve structural flexibility and conformational changes at the level of the protein tertiary structure. Read More

    Metformin promotes neuronal differentiation and neurite outgrowth through AMPK activation in human bone marrow-mesenchymal stem cells.
    Biotechnol Appl Biochem 2017 Aug 9. Epub 2017 Aug 9.
    Department of Biology, College of Natural Science, Chosun University, Gwangju, Korea.
    Metformin is an AMP-activated kinase (AMPK) activator that plays a role in glucose energy metabolism and cell protection. It is widely used to treat several diseases, including type 2 diabetes, cardiovascular diseases, cancer, and metabolic diseases. In this study, we investigated whether AMPK activation upon treatment with metformin may promote neurite outgrowth during the progression of neuronal differentiation in human bone marrow-mesenchymal stem cells (hBM-MSCs). Read More

    Construct a gene-to-metabolite network to screen the key genes of triterpene saponin biosynthetic pathway in Panax notoginseng.
    Biotechnol Appl Biochem 2017 Aug 4. Epub 2017 Aug 4.
    State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, 210009, People's Republic of China.
    Triterpene saponins are main active constituents of Panax notoginseng. Metabolites profiling of 12 triterpene saponins was analyzed by high-performance liquid chromatography-mass spectrometry in leaf, petiole, and root extracts of P. notoginseng. Read More

    SEC-SAXS and HDX-MS: A powerful combination. The case of the calcium-binding domain of a bacterial toxin.
    Biotechnol Appl Biochem 2017 Aug 2. Epub 2017 Aug 2.
    Institut de Biologie Intégrative de la Cellule, UMR 9198, Université Paris-Sud, Orsay, France.
    Small-angle X-ray scattering (SAXS) is a relatively simple experimental technique that provides information on the global conformation of macromolecules in solution, be they fully structured, partially, or extensively unfolded. Size exclusion chromatography in line with a SAXS measuring cell considerably improves the monodispersity and ideality of solutions, the two main requirements of a "good" SAXS sample. Hydrogen/deuterium exchange monitored by mass spectrometry (HDX-MS) offers a wealth of information regarding the solvent accessibility at the local (peptide) level. Read More

    Co-expression of alpha-1 antitrypsin with cytoplasmic domain of v-SNARE in Pichia pastoris: preserving biological activity of alpha-1 antitrypsin.
    Biotechnol Appl Biochem 2017 Aug 1. Epub 2017 Aug 1.
    Department of Biochemistry, Faculty of Basic Science, Tarbiat Modares University, Tehran, Iran.
    Alpha-1-antitrypsin (A1AT) is a major serum protein in human with protease inhibitory activity. Because of its extensive application in medicine, recombinant DNA technology has been considered for its production. The current study is going to examine co-expression of A1AT, and soluble domain of v-SNARE in Pichia pastoris, which can prevent the secretion of A1AT after passing the secretory pathway thoroughly. Read More

    Improvement of amphotericin B production by a newly isolated Streptomyces nodosus mutant.
    Biotechnol Appl Biochem 2017 Aug 1. Epub 2017 Aug 1.
    Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, 310014, People's Republic of China.
    Amphotericin B is an effective antifungal antibiotic. However, its production in the wild-type strain of Streptomyces nodosus is relatively low. In this study, a strain of ZJB 20130827, capable of producing amphotericin B, was isolated and identified as S. Read More

    Role of Q177A and K173A/Q177A substitutions in thermostability and activity of the ELBn12 lipase.
    Biotechnol Appl Biochem 2017 Jul 19. Epub 2017 Jul 19.
    Department of Industrial and Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran.
    Thermostable lipases have many applications in detergent industries and in organic synthesis. There are many ways to improve thermal stability of enzymes, for example, higher hydrophobicity, greater structural packing, higher content of the charged residues, and lower thermolabile ones. In this study, thermolabile Gln (sensitive to higher temperatures) was substituted with Ala in native ELBn12 and mutated K173A lipases to examine its effect on thermal stability and activity of the lipases. Read More

    Role of palmitoylation of cysteine 415 in functional coupling CB1 receptor to Gαi2 protein.
    Biotechnol Appl Biochem 2017 Jul 19. Epub 2017 Jul 19.
    Faculty of Bioscience and Technology for Food Agriculture and Environment, University of Teramo, Teramo, Italy.
    In this study, we investigated the role of CB1 palmitoylation in modulating the functional interaction with G proteins both in the absence and presence of agonist binding. Our data show that the nonpalmitoylated CB1 receptor significantly reduced its association with Gαi2 . The agonist stimulation induced a partial dissociation of Gαi2 proteins from the wild-type receptor, while on the C415A mutant the agonist binding was not able to induce a significant dissociation of Gαi2 from the receptor. Read More

    The relationship of metabolic burden to productivity levels in CHO cell lines.
    Biotechnol Appl Biochem 2017 Jul 6. Epub 2017 Jul 6.
    School of Chemical and Bioprocess Engineering, and Conway Institute of Biomolecular and Biomedical Research, University College Dublin, Dublin, Ireland.
    The growing demand for recombinant therapeutics has driven biotechnologists to develop new production strategies. One such strategy for increasing the expression of heterologous proteins has focused on enhancing cell-specific productivity through environmental perturbations. In this work, the effects of hypothermia, hyperosmolarity, high shear stress, and sodium butyrate treatment on growth and productivity were studied using three (low, medium, and high producing) CHO cell lines that differed in their specific productivities of monoclonal antibody. Read More

    Functional assessment of plant and microalgal lipid pathway genes in yeast to enhance microbial industrial oil production.
    Biotechnol Appl Biochem 2017 Jun 25. Epub 2017 Jun 25.
    Department of Chemical Engineering, Monash University, Clayton, Australia.
    As promising alternatives to fossil-derived oils, microbial lipids are important as industrial feedstocks for biofuels and oleochemicals. Our broad aim is to increase lipid content in oleaginous yeast through expression of lipid accumulation genes and use Saccharomyces cerevisiae to functionally assess genes obtained from oil-producing plants and microalgae. Lipid accumulation genes DGAT (diacylglycerol acyltransferase), PDAT (phospholipid: diacylglycerol acyltransferase), and ROD1 (phosphatidylcholine: diacylglycerol choline-phosphotransferase) were separately expressed in yeast and lipid production measured by fluorescence, solvent extraction, thin layer chromatography, and gas chromatography (GC) of fatty acid methyl esters. Read More

    Improved enzymatic performance of graphene-immobilized β-glucosidase A in the presence of glucose-6-phosphate.
    Biotechnol Appl Biochem 2017 Jun 21. Epub 2017 Jun 21.
    Department of Food and Animal Science, Center of Agroveterinary Sciences, State University of Santa Catarina, Lages, Brazil.
    Optimization of cellulose enzymatic hydrolysis is crucial for cost-effective bioethanol production from lignocellulosic biomass. Enzyme immobilization in solid support allows enzyme recycling for reuse, lowering hydrolysis costs. Graphene is a nanomaterial isolated in 2004, which possesses exceptional properties for biomolecule immobilization. Read More

    Improving thermostability of (R)-selective amine transaminase from Aspergillus terreus through introduction of disulfide bonds.
    Biotechnol Appl Biochem 2017 Jun 21. Epub 2017 Jun 21.
    Department of Biological and Pharmaceutical Engineering, Ningbo Institute of Technology, Zhejiang University, Ningbo, People's Republic of China.
    To improve the thermostability of (R)-selective amine transaminase from Aspergillus terreus (AT-ATA), we used computer software Disulfide by Design and Modelling of Disulfide Bonds in Proteins to identify mutation sites where the disulfide bonds were most likely to form. We obtained three stabilized mutants (N25C-A28C, R131C-D134C, M150C-M280C) from seven candidates by site-directed mutagenesis. Compared to the wild type, the best two mutants N25C-A28C and M150C-M280C showed improved thermal stability with a 3. Read More

    Biosynthesis of novel 7,8-dihydroxyflavone glycoside derivatives and in silico study of their effects on BACE1 inhibition.
    Biotechnol Appl Biochem 2017 Jun 12. Epub 2017 Jun 12.
    Department of Life Science and Biochemical Engineering, SunMoon University, Asan-si, Chungnam, Republic of Korea.
    7,8-Dihydroxyflavone (7,8-DHF) has been conjugated with glucose moiety to produce glucoside derivatives. Three analogues of 7,8-DHF (7-O-β-d-glucosyl-8-hydroxyflavone, 7-hydroxy-8-O-β-d-glucosyl flavone, and 7,8-di-O-β-d-glucosylflavone) have been successfully produced from in vitro reaction using glycosyltransferase of Bacillus licheniformis. Production of these 7,8-DHF derivatives were shifted to cheaper and easier approach in this study by using engineered Escherichia coli BL21 (DE3) ΔpgiΔzwfΔushA cells in which the flow of glucose-6-phospahte toward glycolysis and pentose phosphate pathway and hydrolysis of UDP-α-d-glucose were blocked while directing the carbon flux toward UDP-α-d-glucose by overexpressing UDP-α-d-glucose pathway genes. Read More

    Chemical tools to modulate 2-arachidonoylglycerol biosynthesis.
    Biotechnol Appl Biochem 2017 Jun 6. Epub 2017 Jun 6.
    Department of Molecular Physiology, Leiden Institute of Chemistry, Leiden University, Leiden, The Netherlands.
    2-Arachidonoylglycerol (2-AG) is an important endogenous signaling lipid that activates the cannabinoid receptors (CB1 R and CB2 R), thereby regulating a diverse range of physiological processes including anxiety, appetite, inflammation, memory, pain sensation, and nociception. Diacylglycerol lipases (DAGLs) are the principle enzymes responsible for 2-AG biosynthesis. Recently, the (patho)physiological functions of DAGLs have been explored by both genetic methods and chemical tools. Read More

    Production and application of a thermostable lipase from Serratia marcescens in detergent formulation and biodiesel production.
    Biotechnol Appl Biochem 2017 Apr 26. Epub 2017 Apr 26.
    Centro de Investigación en Biotecnología, Universidad Autónoma del Estado de Morelos, Morelos, México.
    In this study, extracellular lipase was produced by Serratia marcescens wild type and three mutant strains. The maximum lipase activity (80 U/mL) was obtained with the SMRG4 mutant strain using soybean oil. Using a 2(2) factorial design, the lipase production increased 1. Read More

    Production of high concentration of l-lactic acid from oil palm empty fruit bunch by thermophilic Bacillus coagulans JI12.
    Biotechnol Appl Biochem 2017 Apr 24. Epub 2017 Apr 24.
    Department of Industrial biotechnology, Institute of Chemical and Engineering Sciences, 1 Pesek Road, Jurong Island, Singapore 627833.
    Thermophilic Bacillus coagulans JI12 was used to ferment hemicellulose hydrolysate obtained by acid hydrolysis of oil palm empty fruit bunch at 50 °C and pH 6, producing 105.4 g/L of l-lactic acid with a productivity of 9.3 g/L/H by fed-batch fermentation under unsterilized conditions. Read More

    Stilbene content and expression of stilbene synthase genes in cell cultures of Vitis amurensis treated with cinnamic and caffeic acids.
    Biotechnol Appl Biochem 2017 Mar 22. Epub 2017 Mar 22.
    Laboratory of Biotechnology, Institute of Biology and Soil Science, Far Eastern Branch of the Russian Academy of Sciences, Vladivostok, Russia.
    It has previously been shown that exogenous application of p-coumaric acid (CA), a precursor of phenolic compounds, improved stilbene production in cell cultures of Vitis amurensis. This study examines the effect of cinnamic (Cin) and caffeic (Caf) acids, which are also phenolic precursors, on stilbene biosynthesis in the cell cultures. Five stilbenes, t-resveratrol diglucoside, t-piceid (t-resveratrol glucoside), t-resveratrol, t-ε-viniferin, and t-δ-viniferin, were found in the treated and untreated cells. Read More

    Entrapment of DyP-type peroxidase from Pseudomonas fluorescens Pf-5 into Ca-alginate magnetic beads.
    Biotechnol Appl Biochem 2017 Mar 21. Epub 2017 Mar 21.
    Department of Immunology, Microbiology and Physiological Chemistry, Faculty of Biotechnology and Animal Husbandry, West Pomeranian University of Technology, Szczecin, Poland.
    The aim of this study was to investigate the optimal conditions for the immobilization and stabilization of DyP1B dye decolorizing peroxidases type B (DyP1B) from Pseudomonas fluorescens Pf-5 immobilized in Ca-alginate ferromagnetic beads. The immobilized DyP1B was used in the degradation of the Reactive Blue 5 (RB5) synthetic dye. The enzyme was successfully entrapped in a Ca-alginate matrix and showed an encapsulation efficiency of 94%. Read More

    DNAzyme-aptamer or aptamer-DNAzyme paradigm: Biochemical approach for aflatoxin analysis.
    Biotechnol Appl Biochem 2017 Mar 22. Epub 2017 Mar 22.
    Department of Toxicology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
    DNAzyme and aptamer conjugations have already been used for sensitive and accurate detection of several molecules. In this study, we tested the relationship between conjugation orientation of DNAzyme and aflatoxin B1 aptamer and their subsequent peroxidase activity. Circular dichroism (CD) spectroscopy and biochemical analysis were used here to differentiate between these two conjugation patterns. Read More

    Insights into cell-free therapeutic approach: Role of stem cell "soup-ernatant".
    Biotechnol Appl Biochem 2017 Mar 21. Epub 2017 Mar 21.
    Department of Biophysics, PGIMER, Chandigarh, India.
    Current advances in medicine have revolutionized the field of regenerative medicine dramatically with newly evolved therapies for repair or replacement of degenerating or injured tissues. Stem cells (SCs) can be harvested from different sources for clinical therapeutics, which include fetal tissues, umbilical cord blood, embryos, and adult tissues. SCs can be isolated and differentiated into desired lineages for tissue regeneration and cell replacement therapy. Read More

    A comparative investigation of Bombyx mori silk fibroin hydrogels generated by chemical and enzymatic cross-linking.
    Biotechnol Appl Biochem 2017 Feb 21. Epub 2017 Feb 21.
    Polytech Marseille, Department of Biomedical Engineering, Aix-Marseille University, Site Luminy, Marseille, France.
    Fibroin, the major proteinaceous component of the silk fiber produced by larvae of the domesticated silk moth (Bombyx mori), has been widely investigated as a biomaterial for potential applications in tissue engineering and regenerative medicine. Following sol-gel transition, silk fibroin solutions can generate hydrogels that present certain advantages when employed as biomaterials, especially if they are cross-linked. The subject of this study was the self-cross-linking of silk fibroin through a process induced by the enzyme horseradish peroxidase (HRP) in the presence of hydrogen peroxide, a method only recently proposed and scarcely reported. Read More

    Immobilization of horseradish peroxidase on ZnO nanowires/macroporous SiO2 composites for the complete decolorization of anthraquinone dyes.
    Biotechnol Appl Biochem 2017 Feb 21. Epub 2017 Feb 21.
    Faculty of Materials Science and Chemical Engineering, Ningbo University, Ningbo, Zhejiang, People's Republic of China.
    A zinc oxide (ZnO) nanowires/macroporous silicon dioxide composite was used as support to immobilize horseradish peroxidase (HRP) simply by in situ cross-linking method. As cross-linker was adsorbed on the surface of ZnO nanowires, the cross-linked HRP was quite different from the traditional cross-linking enzyme aggregates on both structure and catalytic performance. Among three epoxy compounds, diethylene glycol diglycidyl ether (DDE) was the best cross-linker, with which the loading amount of HRP with pI of 5. Read More

    Enzymatic characterization and functional implication of two structurally different isocitrate dehydrogenases from Xylella fastidiosa.
    Biotechnol Appl Biochem 2017 Feb 21. Epub 2017 Feb 21.
    The Research Center of Life Omics and Health and Anhui Provincial Key Laboratory of the Conservation and Exploitation of Biological Resources, Anhui Normal University, Wuhu, Anhui, People's Republic of China.
    Isocitrate dehydrogenase (IDH) is a key enzyme at the critical junction between the tricarboxylic acid cycle and the glyoxylate cycle. Most bacteria have only one IDH, while a few contain two IDH isozymes. The coexistence of two different type IDHs in one organism was little known. Read More

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