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    2071 results match your criteria Biotechnology and Applied Biochemistry[Journal]

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    A dual-label time-resolved fluorescence immunoassay for the simultaneous determination of carcinoembryonic antigen and squamous cell carcinoma antigen.
    Biotechnol Appl Biochem 2018 Apr 20. Epub 2018 Apr 20.
    Guangzhou Youdi Bio-technology Co., Ltd., Guangzhou, People's Republic of China.
    Among women worldwide, cervical cancer is the second-most common cancer, and cervical smears and DNA detection have low sensitivity or are too expensive. The concentrations of carcinoembryonic antigen (CEA) and squamous cell carcinoma (SCC) in the serum were detected using a sandwich immunoassay. The CEA and SCC in the serum were captured by anti-CEA and anti-SCC antibodies. Read More

    Characterization and epitope mapping of a panel of monoclonal antibodies against HIV-1 matrix protein.
    Biotechnol Appl Biochem 2018 Apr 17. Epub 2018 Apr 17.
    State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health, Xiamen University, Xiamen, People's Republic of China.
    The HIV-1 Gag precursor protein (p55) is the main structural protein comprising the matrix (MA/p17), capsid (CA/p24), and nucleocapsid (NC/p7) proteins, and is uniquely responsible for virion assembly within the virus life cycle. The MA protein plays a critical role in plasma membrane targeting and envelope glycoprotein (Env) uptake during virion assembly. Yet, when viral infection occurs, the MA protein may also be involved in virion uncoating, dissociating from the plasma membrane, and participating in the nuclear importation process. Read More

    Amidated Scolopin-2 inhibits proliferation and induces apoptosis of Hela cells in vitro and in vivo.
    Biotechnol Appl Biochem 2018 Apr 12. Epub 2018 Apr 12.
    Jiangsu Province Key Laboratory for Molecular and Medical Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing, People's Republic of China.
    This study aimed to investigate the effect of Scolopin-2, a cationic antimicrobial peptide from centipede venoms, and amidated Scolopin-2 on Hela cell viability in vitro and in vivo. The cellular proliferation was investigated with the MTT assay. Confocal laser scanning, flow cytometry, and Western blot analysis were employed to localize Scolopin-2-NH in Hela cells and to study the caused cells apoptosis. Read More

    Identification and characterization of a novel esterase from Thauera sp.
    Biotechnol Appl Biochem 2018 Apr 6. Epub 2018 Apr 6.
    Research Institute of Tropical Forestry, Chinese Academy of Forestry, Guangzhou, 510520, People's Republic of China.
    A novel esterase gene TLip was identified from the strain Thauera sp. and expressed at high levels in Escherichia coli. The TLip protein shared the highest identity (48%) to esterase TesA from Pseudomonas aeruginosa when compared to enzymes with reported properties. Read More

    Production and characterization of a human lysosomal recombinant iduronate-2-sulfatase produced in Pichia pastoris.
    Biotechnol Appl Biochem 2018 Apr 6. Epub 2018 Apr 6.
    Institute for the Study of Inborn Errors of Metabolism, Faculty of Science, Pontificia Universidad Javeriana, Bogotá, Colombia.
    Hunter syndrome (Mucopolysaccharidosis II, MPS II) is an X-linked lysosomal storage disease produced by the deficiency of the lysosomal enzyme iduronate-2-sulfatase (IDS). Currently, MPS II patients are mainly treated with enzyme replacement therapy (ERT) using recombinant enzymes produced in mammalian cells. As an alternative, several studies have shown the production of active and therapeutic forms of lysosomal proteins in microorganisms. Read More

    Gemcitabine induces apoptosis and autophagy via the AMPK/mTOR signaling pathway in pancreatic cancer cells.
    Biotechnol Appl Biochem 2018 Mar 25. Epub 2018 Mar 25.
    Department of General Surgery, Second Affiliated Hospital Zhejiang University School of Medicine, Hangzhou, People's Republic of China.
    Gemcitabine (GEM)-based chemotherapy is a commonly used treatment for pancreatic cancer. However, acquired drug resistance, a major problem in pancreatic cancer treatment, causes a reduction in the survival rate of patients with cancer. In this study, we attempted to reveal the molecular mechanism of GEM resistance. Read More

    Structural basis of noncanonical polyphenol oxidase activity in DLL-II: A lectin from Dolichos lablab.
    Biotechnol Appl Biochem 2018 Mar 23. Epub 2018 Mar 23.
    Department of Molecular Nutrition, CSIR-Central Food Technological Research Institute, Mysore, India.
    Lectins known to possess an additional enzymatic function are called leczymes. Previous studies reported a unique polyphenol oxidase (PPO) activity in DLL-II-a leczyme from Dolichos lablab. DLL-II shares a high sequence and structural homology with DBL-another leczyme from Dolichos biflorus. Read More

    Kinetic models for the effect of temperature on flavonoid production in liquid submerged fermentation by Phellinus baumii.
    Biotechnol Appl Biochem 2018 Mar 23. Epub 2018 Mar 23.
    Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai, People's Republic of China.
    Kinetic models and temperature control strategy were established to reflect the effect of temperature (22 °C-30 °C) on flavonoid production of Phellinus baumii (P. baumii) in 6-L fermentor. A modified Logistic equation, Hinshelwood model, and Luedeking Piret equation were used to describe mycelial growth and product formation. Read More

    The psychrotrophic yeast Sporobolomyces roseus LOCK 1119 as a source of a highly active aspartic protease for the in vitro production of antioxidant peptides.
    Biotechnol Appl Biochem 2018 Mar 23. Epub 2018 Mar 23.
    Institute of Technical Biochemistry, Lodz University of Technology, Lodz, Poland.
    A psychrotrophic yeast strain producing a cold-adapted protease at low temperature was classified as Sporobolomyces roseus. In standard YPG medium, S. roseus LOCK 1119 synthesized an extracellular protease with an activity of approximately 560 U/L. Read More

    Development and characterization of a camelid single-domain antibody directed to human CD22 biomarker.
    Biotechnol Appl Biochem 2018 Mar 15. Epub 2018 Mar 15.
    National Cell Bank of Iran, Pasteur Institute of Iran, Tehran, Iran.
    CD22 is a B-cell-specific trans-membrane glycoprotein, which is found on the surface of the most B cells and modulates their function, survival, and apoptosis. Recently, targeting this cell surface biomarker in B-cell malignancies and disorders has attracted a lot of attention. The variable domain of camelid single-chain antibodies (VHH, nanobody) is a form of antibodies with novel properties including small size (15-17 kDa), thermal and chemical stability, high affinity and homology to human antibody sequences. Read More

    Improved production, purification, and characterization of biosurfactants produced by Serratia marcescens SM3 and its isogenic SMRG-5 strain.
    Biotechnol Appl Biochem 2018 Feb 19. Epub 2018 Feb 19.
    Laboratorio de Biotecnología Ambiental, Centro de Investigación en Biotecnología, Universidad Autónoma del Estado de Morelos, Cuernavaca, México.
    In this study, the biosurfactants (Bs) production of two Serratia marcescens strains (SM3 and its isogenic SMRG-5 strain) was improved and the tenso-active agents were purified and characterized. A 2 factorial design was used to evaluate the effect of nitrogen and carbon sources on the surface tension (ST) reduction and emulsion index (EI ) of the produced Bs. Optimum Bs production by SM3 was achieved at high concentrations of carbon and nitrogen, reducing ST to 26. Read More

    Efficient bioconversion of oleuropein from olive leaf extract to antioxidant hydroxytyrosol by enzymatic hydrolysis and high-temperature degradation.
    Biotechnol Appl Biochem 2018 Feb 10. Epub 2018 Feb 10.
    Key Laboratory of Forest Genetics and Biotechnology of the Ministry of Education, Nanjing, People's Republic of China.
    Hydroxytyrosol (HT), a powerful antioxidant, clears free radicals and exhibits many biological activities. Because contents of HT are low in natural sources, bioconversion of oleuropein (OLE) to HT is of increasing interest. A biotechnological process was investigated to produce HT from OLE presented in olive leaf extract. Read More

    Extracting functional groups of ALLINI to design derivatives of FDA-approved drugs: Inhibition of HIV-1 integrase.
    Biotechnol Appl Biochem 2018 Feb 6. Epub 2018 Feb 6.
    Department of Pharmaceutical Technology and Biochemistry, Faculty of Chemistry, Gdansk University of Technology, Gdansk, Poland.
    HIV-1 integrase (IN) is crucial for integration of viral DNA into the host genome and a promising target in development of antiretroviral inhibitors. In this work, six new compounds were designed by linking the structures of two different class of HIV-1 IN inhibitors (active site binders and allosteric IN inhibitors (ALLINIs)). Among newly designed compounds, INRAT10b was found most potent HIV-1 IN inhibitor considering different docking results. Read More

    Process optimization of the extraction condition of β-amylase from brewer's malt and its application in the maltose syrup production.
    Biotechnol Appl Biochem 2018 Feb 2. Epub 2018 Feb 2.
    Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, People's Republic of China.
    β-Amylase is of important biotechnological aid in maltose syrup production. In this study, the extraction condition of β-amylase from brewer's malt and the optimal dosage of β-amylase in maltose syrup production were optimized using response surface methodology and uniform design method. The optimal extraction condition of β-amylase from brewer's malt was composed of 1:17 (g/v) material/liquid ratio, 44°C extraction temperature, pH 6. Read More

    Advances in ginsenoside biosynthesis and metabolic regulation.
    Biotechnol Appl Biochem 2018 Jan 29. Epub 2018 Jan 29.
    Tianjin Key Laboratory for Modern Drug Delivery and High Efficiency, School of Pharmaceutical Science and Technology, Tianjin University, Tianjin, People's Republic of China.
    In this paper, we reviewed the advances in ginsenoside biosynthesis and metabolic regulation. To begin with, the application of elicitors in the ginsenoside biosynthesis was discussed. Methyl jasmonate (MJ) and analogues have the best effect on accumulation of ginsenoside compared with other elicitors, and few biotic elicitors are applied in Panax genus plants tissue culture. Read More

    Effect of linkers on immobilization of scFvs with biotin-streptavidin interaction.
    Biotechnol Appl Biochem 2018 Jan 29. Epub 2018 Jan 29.
    Department of Chemical and Biomolecular Engineering, University of Maryland, College Park, MD, USA.
    Single-chain variable fragment antibodies (scFvs) are attractive for use in applications that require high specificity and binding to a target, such as biosensors. Previously, we demonstrated that a variety of scFvs can be immobilized onto a streptavidin surface through in vivo biotinylation of the biotin carboxyl carrier protein (BCCP) or smaller AviTag fused to the scFvs. However, the BCCP constructs showed better immobilization than the AviTag constructs. Read More

    Identification of small molecular inhibitors for efflux protein Rv2688c of Mycobacterium tuberculosis.
    Biotechnol Appl Biochem 2018 Jan 29. Epub 2018 Jan 29.
    Molecular Modeling Research Laboratory, Department of Chemistry, University College of Science, Osmania University, Hyderabad, India.
    Mycobacterium tuberculosis (Mtb) is the pathogen, which causes tuberculosis. The development of multidrug-resistant and extensively drug-resistant strains in Mtb is due to an efflux mechanism of antibiotics in the bacteria. The efflux pump proteins in the bacteria are implicated in the active efflux of antibiotics. Read More

    Efficient production and characterization of homopolymeric poly(3-hydroxyvalerate) produced by Bacillus strain PJC48.
    Biotechnol Appl Biochem 2018 Jan 29. Epub 2018 Jan 29.
    School of Chemistry and Chemical Engineering/Key Laboratory for Green Processing of Chemical Engineering of Xinjiang Bingtuan, Shihezi University, Shihezi, Xinjiang, People's Republic of China.
    Aliphatic polyester, poly(3-hydroxyvalerate) (PHV), is commonly produced as a granular component in bacterial cells of various species. Based on 16S rDNA gene sequence analysis, strain PJC48 was identified as a Bacillus species. The current study is aimed to screen for a high-yield strain that can produce PHV efficiently and to increase PHV product yield by optimizing the fermentative process. Read More

    Combat biofilm by bacteriostatic aptamer-functionalized graphene oxide.
    Biotechnol Appl Biochem 2018 Jan 22. Epub 2018 Jan 22.
    State Key Laboratory of Developmental Biology of Freshwater Fish, College of Life Sciences, Hunan Normal University, Changsha, Hunan, People's Republic of China.
    Biofilms are the main reason for a large number deaths and high health costs. Their better protection compared to planktonic form against conventional antibiotics leads to poor treatment efficiency. Nanoagent-targeted delivery is a promising avenue for disease therapeutic, but its application targeting biofilms has not been reported currently. Read More

    Secretory expression of negative regulatory region of human Notch1 in Escherichia coli and preparation of a functional polyclonal antibody.
    Biotechnol Appl Biochem 2018 Jan 17. Epub 2018 Jan 17.
    Key Laboratory of Pu-er Tea Science, Ministry of Education, Yunnan Agricultural University, Kunming, People's Republic of China.
    Notch signaling is a highly conserved pathway existed in multicellular organisms. It plays roles in normal human body development, human cancer initiation, progression and metastasis. The Notch negative regulatory region (NRR) is critical for Notch signaling, and cleavage at the S2 site in the NRR ultimately leads to the activation of Notch signaling. Read More

    4-Phenylbutyric acid presents therapeutic effect on osteoarthritis via inhibiting cell apoptosis and inflammatory response induced by endoplasmic reticulum stress.
    Biotechnol Appl Biochem 2018 Jan 11. Epub 2018 Jan 11.
    Department of Orthopedics, Xiaoshan Traditional Chinese Medical Hospital, Hangzhou, 311201, Zhejiang, People's Republic of China.
    Osteoarthritis (OA) is a common bone and joint disease with a wild range of risk factors, which is associated with endoplasmic reticulum (ER) stress. The aim of our study was to discuss the possible mechanism of ER stress associated with OA in vivo and explore novel therapeutic method against OA. OA-induced damages in cartilage tissues were evaluated by HE, Safranin O/fast green, and TUNEL staining. Read More

    Fabrication of silver chloride nanoparticles using a plant serine protease in combination with photoactivation and investigation of their biological activities.
    Biotechnol Appl Biochem 2018 Jan 3. Epub 2018 Jan 3.
    Department of Medical Technology, Faculty of Allied Health Sciences, Thammasat University, Rangsit Center, Pathum Thani, Thailand.
    Recently, the development of "green" methods for fabrication of silver nanoparticles (Ag-NPs) has been emphasized, in view of their environmental safety, feasibility, and low cost. In this study, a serine protease, EuP-82 from Euphorbia cf. lactea latex, was used to fabricate silver chloride nanoparticles (AgCl-NPs) in phosphate-buffered saline (pH 7. Read More

    Semiquantitative dot-blot immunogold assay for specific detection of white spot syndrome virus.
    Biotechnol Appl Biochem 2018 Jan 4. Epub 2018 Jan 4.
    Department of Molecular Biotechnology and Bioinformatics, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla, Thailand.
    A dot-blot immunogold assay (DBIA) was developed to detect white spot syndrome virus (WSSV) using the polyclonal antibody VP26 (anti-VP26). The anti-VP26 was immobilized on gold nanoparticles (Ab-AuNPs), and a nitrocellulose membrane was used as a detection pad. When the target WSSV bound to the Ab-AuNPs a reddish dot appeared on the surface of the membrane used within 2-5 Min, which could be seen with the naked eye. Read More

    In situ downstream strategies for cost-effective bio/surfactant recovery.
    Biotechnol Appl Biochem 2018 Jan 3. Epub 2018 Jan 3.
    Faculty of Life and Health Sciences, School of Biomedical Sciences, University of Ulster, Coleraine, N. Ireland, UK.
    Since 60-80% of total costs of production are usually associated with downstream collection, separation, and purification processes, it has become advantageous to investigate how to replace traditional methods with efficient and cost-effective alternative techniques for recovery and purification of biosurfactants. In the traditional techniques, large volumes of organic solvents are usually used for increasing production cost and the overall environmental burden. In addition, traditional production and separation methods typically carried out in batch cultures reduce biosurfactant yields due to product inhibition and lower biosurfactants activity as a result of interaction with the organic solvents used. Read More

    Improved pulp bleaching potential of Bacillus subtilis WB800 through overexpression of three lignolytic enzymes from various bacteria.
    Biotechnol Appl Biochem 2017 Dec 29. Epub 2017 Dec 29.
    Department of Biology, Faculty of Science, Karadeniz Technical University, Trabzon, Turkey.
    A chemical bleaching process of paper pulps gives off excessive amount of chlorinated organic wastes mostly released to environment without exposing complete bioremediaton. Recent alternative and eco-friendly approaches toward pulp bleaching appear more responsive to environmental awareness. Here we report, direct use of a recombinant Bacillus subtilis bacterium for pulp bleaching, endowed with three ligninolytic enzymes from various bacteria. Read More

    Human neonatal Fc receptor as a new potential antibody binding protein for antibody immobilization.
    Biotechnol Appl Biochem 2017 Dec 27. Epub 2017 Dec 27.
    Institute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia, Pulau Pinang, Malaysia.
    A critical challenge in producing an antibody-based assay with the highest reproducibility and sensitivity is the strategy to immobilize antibodies to solid phase. To date, numerous methods of antibody immobilization were reported but each was subjected to its advantages and limitations. The current study proposes a new potential antibody binding protein, the human neonatal fragment crystallizable (Fc) receptor. Read More

    Investigation of autophagy and differentiation of myenteric interstitial cells of Cajal in the pathogenesis of gastric motility disorders in rats with functional dyspepsia.
    Biotechnol Appl Biochem 2017 Dec 23. Epub 2017 Dec 23.
    The First Affiliated Hospital of Guangxi Medical University, Nanning, People's Republic of China.
    Interstitial cells of Cajal (ICC), especially myenteric interstitial cells of Cajal (ICC-MY), are key to gastrointestinal motility. However, their role in the pathogenesis of functional dyspepsia (FD) is unclear. Therefore, autophagy and differentiation of ICC-MY were investigated to elucidate the pathogenesis of gastric motility disorder in FD. Read More

    Effects of celastrol on Tau hyperphosphorylation and expression of HSF-1 and HSP70 in SH-SY5Y neuroblastoma cells induced by amyloid-β peptides.
    Biotechnol Appl Biochem 2017 Dec 22. Epub 2017 Dec 22.
    Department of Clinical Lab, Shanghai Gongli Hospital, the Second Military Medical University, Pudong New District, Shanghai, People's Republic of China.
    To observe the effects of celastrol on Tau hyperphosphorylation induced by amyloid-β peptides (Aβ) in SH-SY5Y neuroblastoma cells, the changes of Tau hyperphosphorylation and the expression of heat shock protein 90 (HSP90), HSP70, and heat shock factor 1 (HSF-1) in SH-SY5Y cells treated with Aβ and celastrol were measured. Tau hyperphosphorylation and HSP90 expression induced by Aβ was also measured by Western blotting after HSP70 or HSF-1 knockdown by siRNA. The interaction between HSP70 and Tau or HSP70 and carboxyl terminus of HSP70 interacting protein (CHIP) was measured by co-immunoprecipitation. Read More

    Peptide (Lys-Leu) and amino acids (Lys and Leu) supplementations improve physiological activity and fermentation performance of brewer's yeast during very high-gravity (VHG) wort fermentation.
    Biotechnol Appl Biochem 2017 Dec 22. Epub 2017 Dec 22.
    School of Food Science and Engineering, South China University of Technology, Guangzhou, People's Republic of China.
    Lys and Leu were generally considered as the key amino acids for brewer's yeast during beer brewing. In the present study, peptide Lys-Leu and a free amino acid (FAA) mixture of Lys and Leu (Lys + Leu) were supplemented in 24 °P wort to examine their effects on physiological activity and fermentation performance of brewer's yeast during very high-gravity (VHG) wort fermentation. Results showed that although both peptide Lys-Leu and their FAA mixture supplementations could increase the growth and viability, intracellular trehalose and glycerol content, wort fermentability, and ethanol content for brewer's yeast during VHG wort fermentation, and peptide was better than their FAA mixture at promoting growth and fermentation for brewer's yeast when the same dose was kept. Read More

    Noncoded amino acids in protein engineering: Structure-activity relationship studies of hirudin-thrombin interaction.
    Biotechnol Appl Biochem 2018 Jan 16;65(1):69-80. Epub 2018 Jan 16.
    Laboratory of Protein Chemistry, Department of Pharmaceutical & Pharmacological Sciences, University of Padua, Padua, Italy.
    The advent of recombinant DNA technology allowed to site-specifically insert, delete, or mutate almost any amino acid in a given protein, significantly improving our knowledge of protein structure, stability, and function. Nevertheless, a quantitative description of the physical and chemical basis that makes a polypeptide chain to efficiently fold into a stable and functionally active conformation is still elusive. This mainly originates from the fact that nature combined, in a yet unknown manner, different properties (i. Read More

    Immobilization of carbonic anhydrase on polyvinylidene fluoride membranes.
    Biotechnol Appl Biochem 2017 Dec 9. Epub 2017 Dec 9.
    School of Chemistry and Chemical Engineering, Beijing Institute of Technology, Beijing, People's Republic of China.
    In recent years, the application of carbonic anhydrase (CA) in CO removal has attracted great interest. However, obtaining high enzyme recovery activity is difficult in existing immobilization techniques. In this work, water plasma-treated poly(vinylidene fluoride) (PVDF) membranes were modified via 3-aminopropyl triethoxy silane (KH550) or γ-(2, 3-epoxypropoxy) propyl trimethoxy silane (KH560), and then CA was attached. Read More

    Application of aqueous two-phase micellar system to improve extraction of adenoviral particles from cell lysate.
    Biotechnol Appl Biochem 2017 Dec 4. Epub 2017 Dec 4.
    Department of Biochemical and Pharmaceutical Technology, School of Pharmaceutical Sciences, University of São Paulo, São Paulo, Brazil.
    Viral vectors are important in medical approaches, such as disease prevention and gene therapy, and their production depends on efficient prepurification steps. In the present study, an aqueous two-phase micellar system (ATPMS) was evaluated to extract human adenovirus type 5 particles from a cell lysate. Adenovirus was cultured in human embryonic kidney 293 (HEK-293) cells to a concentration of 1. Read More

    Novel antioxidant and anti-inflammatory peptides from the Siamese crocodile (Crocodylus siamensis) hemoglobin hydrolysate.
    Biotechnol Appl Biochem 2017 Nov 22. Epub 2017 Nov 22.
    Protein and Proteomics Research Center for Commercial and Industrial Purposes (ProCCI), Faculty of Science, Khon Kaen University, Khon Kaen, Thailand.
    Novel antioxidant and anti-inflammatory peptides were isolated from hydrolysates of Siamese crocodile (Crocodylus siamensis) hemoglobin. C. siamensis hemoglobin hydrolysates (CHHs) were obtained by pepsin digestion at different incubation times (2, 4, 6, and 8 H) at 37 °C and subjected to antioxidant and anti-inflammatory activity assessment. Read More

    Structure-based analysis of Clot as a thioredoxin-related protein of 14 kDa in Drosophila via experimental and computational approaches.
    Biotechnol Appl Biochem 2017 Oct 28. Epub 2017 Oct 28.
    Department of Medical Biotechnology, Soonchunhyang University, Asan, 31538, Korea.
    The clot gene is required for the biosynthesis of drosopterins, the red components of Drosophila eye pigments. However, the enzymatic role of Clot in Drosophila eye pigment formation and the molecular mechanisms underlying Clot function are not fully elucidated. In this study, we cloned and characterized Clot derived from Drosophila cDNA, and results showed that Clot exhibited ∼30% sequence identity with mammalian TRP14. Read More

    Large-scale purification of high purity α1-antitrypsin from Cohn Fraction IV with virus inactivation by solvent/detergent and dry-heat treatment.
    Biotechnol Appl Biochem 2017 Oct 26. Epub 2017 Oct 26.
    Beijing Key Laboratory of Blood Safety and Supply Technologies, Beijing Institute of Transfusion Medicine, Beijing, People's Republic of China.
    α1-Antitrypsin (AAT) is widely used to treat patients with congenital AAT deficiency. Cohn Fraction IV (Cohn F IV) is normally discarded during the manufacturing process of albumin but contains approximately 33% of plasma AAT. We established a new process for large-scale purification of AAT from it. Read More

    Impact of mycolic acid deficiency on cells of Corynebacterium glutamicum ATCC13869.
    Biotechnol Appl Biochem 2017 Oct 26. Epub 2017 Oct 26.
    School of Biotechnology, Jiangnan University, Wuxi, People's Republic of China.
    Mycolic acid (MA) plays important role in Corynebacterium glutamicum, but the key enzymes in the biosynthetic pathway of MA in C. glutamicum ATCC13869 have not been characterized. Since the locus BBD29_RS14045 in C. Read More

    Trans-acting oligodeoxythymidine phosphorothioate triester reagents for transient transfection optimized and facilitated by a high-throughput microbioreactor system.
    Biotechnol Appl Biochem 2017 Oct 10. Epub 2017 Oct 10.
    Division of Biotechnology Review and Research II, OBP, CDER, Food and Drug Administration, Silver Spring, MD, USA.
    A rapid and cost-effective transient transfection method for mammalian cells is essential for screening biopharmaceuticals in early stages of development. A library of 25 amphipathic trans-acting oligodeoxythymidine phosphorothioate triester (dTtaPS) transfection reagents, carrying positively charged and lipophilic groups, has been constructed for this purpose. High-throughput screening of the library, using an imaging cytometer and an automated microbioreactor system, has led to the identification of dTtaPS as a potent transfection reagent. Read More

    Biosensors: Classifications, medical applications, and future prospective.
    Biotechnol Appl Biochem 2017 Oct 10. Epub 2017 Oct 10.
    Faculty of Applied Medical Sciences, Department of Medical Laboratory Technology, King Abdulaziz University, Jeddah, Kingdom of Saudi Arabia.
    Biosensors are devices that combine a biological material with a suitable platform for detection of pathogenic organisms, carcinogenic, mutagenic, and/or toxic chemicals or for reporting a biological effect. In recent years, an enormous number of different types of biosensors have been constructed and developed for several medical applications. The reason for that was primarily due to the numerous advantages and applications that can be offered by biosensors. Read More

    The BMP antagonist Noggin is produced by osteoblasts in response to the presence of prostate cancer cells.
    Biotechnol Appl Biochem 2017 Oct 5. Epub 2017 Oct 5.
    Department of Oncology and Metabolism, Medical School, University of Sheffield, Sheffield, United Kingdom.
    Bone metastasis is a key event responsible for morbidity in prostate cancer patients. Interactions between prostate cancer cells and the bone microenvironment facilitate survival of tumor cells and alter bone turnover, a process that is thought to enhance the growth of metastases in this site. This study aimed to test the hypothesis that the presence of tumors cells increases transforming growth factor beta (TGF-β) signaling in bone and that this regulates the proliferation and differentiation of osteoblastic lineage cells in metastatic sites. Read More

    Construction of tissue-engineered lymphatic vessel using human adipose derived stem cells differentiated lymphatic endothelial like cells and decellularized arterial scaffold: A preliminary study.
    Biotechnol Appl Biochem 2017 Oct 5. Epub 2017 Oct 5.
    Department of Microsurgery and Orthopedic Trauma, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, People's Republic of China.
    We have previously demonstrated that human adipose-derived stem cells (hADSCs) can be differentiated into lymphatic endothelial like cells. The purpose of this study was to investigate the feasibility of utilizing the induced lymphatic endothelial like cells and decellularized arterial scaffold to construct the tissue-engineered lymphatic vessel. The hADSCs were isolated from adipose tissue in healthy adults and were characterized the multilineage differentiation potential. Read More

    Progress in biopharmaceutical development.
    Biotechnol Appl Biochem 2017 Oct 3. Epub 2017 Oct 3.
    Department of Bioengineering, Institute of Biotechnology and Antibiotics, Warsaw, Poland.
    Since its introduction in 1982, biopharmaceutical drugs have revolutionized the treatment of a broad spectrum of diseases and are increasingly used in nearly all branches of medicine. In recent years, the biopharmaceuticals market has developed much faster than the market for all drugs and is believed to have great potential for further dynamic growth because of the tremendous demand for these drugs. Biobetters, which contain altered active pharmaceutical ingredients with enhanced efficacy, will play an important role in the development of biopharmaceuticals. Read More

    FleQ regulates both the type VI secretion system and flagella in Pseudomonas putida.
    Biotechnol Appl Biochem 2017 Oct 2. Epub 2017 Oct 2.
    State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, People's Republic of China.
    Based on the analysis of sodium dodecyl sulfate-polyacrylamide gel electrophoresis and mass spectrometry, flagellin FliC and a key member of the type VI secretion system Hcp1 were found to be secreted from Pseudomonas putida KT2442 cells in the late exponential phase, but in the early exponential phase only FliC was secreted. Relevant genes fleQ and rpoN were then individually deleted in P. putida KT2442, and the expression and transcription of fliC and hcp1 in these mutant strains were analyzed. Read More

    Studying the TRAF2 binding to model membranes: The role of subunits dissociation.
    Biotechnol Appl Biochem 2018 Jan;65(1):38-45
    Department of Experimental Medicine and Surgery, University of Rome Tor Vergata, Rome, Italy.
    The ability of a C-terminal truncated form of TRAF2 to bind synthetic vesicles has been quantitatively studied by steady-state fluorescence energy transfer from the protein to large unilamellar vesicles (LUVs) prepared with different lipid mixtures. The dissociation constants, the free energy of binding, and the average number of phospholipids interacting with truncated TRAF2 have been evaluated from the corresponding binding curves. The results indicate that the protein strongly interacts with the lipid bilayer, preferentially in the monomeric state. Read More

    Amine oxidase from Euphorbia characias: Kinetic and structural characterization.
    Biotechnol Appl Biochem 2018 Jan 23;65(1):81-88. Epub 2017 Oct 23.
    Department of Sciences of Life and Environment, University of Cagliari, Monserrato, Cagliari, Italy.
    This minireview focuses on a plant copper/2,4,5-trihydroxyphenyl alanine quinone amine oxidase isolated from the latex of the shrub Euphorbia characias (ELAO). This enzyme has been investigated in terms of both molecular structure and kinetic mechanism. The characterization of this enzyme allowed us to identify specific amino acids and domains that play a key role in modulating substrate access into the active site not only for ELAO but also for other plant and mammalian amine oxidases. Read More

    Working at the membrane interface: Ligand-induced changes in dynamic conformation and oligomeric structure in human aromatase.
    Biotechnol Appl Biochem 2018 Jan 23;65(1):46-53. Epub 2017 Oct 23.
    Department of Life Sciences and Systems Biology, University of Torino, Torino, Italy.
    Aromatase catalyzes the biosynthesis of estrogens from androgens. Owing to the physiological importance of this conversion of lipophilic substrates, the interaction with the lipid bilayer for this cytochrome P450 is crucial for its dynamics that must allow an easy access to substrates and inhibitors. Here, the aromatase-anastrozole interaction is studied by combining computational methods to identify possible access/egress routes with the protein inserted in the membrane and experimental tools aimed at the investigation of the effect of the inhibitor on the protein conformation. Read More

    Construction and application of an electrochemical biosensor based on an endotoxin aptamer.
    Biotechnol Appl Biochem 2017 Sep 9. Epub 2017 Sep 9.
    College of Pharmaceutical Science, ZheJiang University, HangZhou, People's Republic of China.
    An electrochemical biosensor that used an aptamer as a biological element was constructed to detect endotoxin. Biolayer interferometry was used to obtain the affinity constant of an aptamer for lipopolysaccharide, which had an equilibrium dissociation constant of 22.9 nM. Read More

    Improving properties of silk sericin membranes via enzymatic oxidation with laccase and TEMPO.
    Biotechnol Appl Biochem 2017 Sep 7. Epub 2017 Sep 7.
    Key Laboratory of Science and Technology of Eco-Textile, Ministry of Education, Jiangnan University, Wuxi, People's Republic of China.
    Silk sericin has excellent features of antioxidant ability and good cytocompatibility; however, high water solubility and poor mechanical properties have restricted its application in biomedical fields. In this study, aimed at improving the mechanical properties of a regenerated silk sericin, the primary hydroxy groups in silk sericin were enzymatically oxidized by using laccase and 2,2',6,6'-tetramethylpiperidine-N-oxyl (TEMPO), and the generated reactive groups then reacted further with the amino groups in the sericin chains. The efficacy of the enzymatic cross-linking was evaluated by means of determination of amino groups, SDS polyacrylamide gel electrophoresis, Fourier transform infrared (FTIR) spectroscopy, and size exclusion chromatography. Read More

    An activity transition from NADH dehydrogenase to NADH oxidase during protein denaturation.
    Biotechnol Appl Biochem 2017 Sep 7. Epub 2017 Sep 7.
    Department of Chemistry, Rutgers University-Camden, Camden, NJ, USA.
    A decrease in the specific activity of an enzyme is commonly observed when the enzyme is inappropriately handled or is stored over an extended period. Here, we reported a functional transition of an FMN-bound diaphorase (FMN-DI) that happened during the long-term storage process. It was found that FMN-DI did not simply lose its β-nicotinamide adenine diphosphate (NADH) dehydrogenase activity after a long-time storage, but obtained a new enzyme activity of NADH oxidase. Read More

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