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    2051 results match your criteria Biotechnology and Applied Biochemistry[Journal]

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    Secretory expression of negative regulatory region of human Notch1 in E.coli and preparation of a functional polyclonal antibody.
    Biotechnol Appl Biochem 2018 Jan 17. Epub 2018 Jan 17.
    Key Laboratory of Pu-er Tea Science, Ministry of Education, Yunnan Agricultural University, Kunming, 650201, China.
    Notch signaling is a highly conserved pathway existed in multicellular organisms. It plays roles in normal human body development, human cancer initiation, progression and metastasis. The Notch negative regulatory region (NRR) is critical for Notch signaling, and cleavage at the S2 site in the NRR ultimately leads to the activation of Notch signaling. Read More

    4-phenylbutyric acid presents therapeutic effect on osteoarthritis via inhibiting cell apoptosis and inflammatory response induced by endoplasmic reticulum stress.
    Biotechnol Appl Biochem 2018 Jan 11. Epub 2018 Jan 11.
    Department of Orthopedics, Xiaoshan Traditional Chinese Medical Hospital, Hangzhou, 311201, Zhejiang, P.R. China.
    Osteoarthritis (OA) is a common bone and joint disease with a wild range of risk factors, which is associated with endoplasmic reticulum (ER) stress. The aim of our study was to discuss the possible mechanism of ER stress associated with OA in vivo and explore novel therapeutic method against OA. OA-induced damages in cartilage tissues were evaluated by HE, Safranin O/fast green and TUNEL staining. Read More

    Biotechnol Appl Biochem 2018 Jan 3. Epub 2018 Jan 3.
    Department of Medicine, Campus Bio-Medico University of Rome, Via Alvaro del Portillo 21, Rome, Italy.
    In the recent years it has become evident that most expressed and not yet expressed proteins do not show a stable folding. This apparently confusing disorder is instead a fundamental property of polypeptides enabling them to interact with different partners playing many different functions. This article is protected by copyright. Read More

    Fabrication of silver chloride nanoparticles using a plant serine protease in combination with photo-activation and investigation of their biological activities.
    Biotechnol Appl Biochem 2018 Jan 3. Epub 2018 Jan 3.
    Department of Medical Technology, Faculty of Allied Health Sciences, Thammasat University, Rangsit Center, Pathum Thani, Thailand.
    Recently, the development of 'green' methods for fabrication of silver nanoparticles (Ag-NPs) has been emphasized, in view of their environmental safety, feasibility and low cost. In this study, a serine protease, EuP-82 from Euphorbia cf. lactea latex, was used to fabricate silver chloride nanoparticles (AgCl-NPs) in phosphate-buffered saline (pH 7. Read More

    Semi-quantitative Dot-blot Immunogold Assay for Specific Detection of White Spot Syndrome Virus (WSSV).
    Biotechnol Appl Biochem 2018 Jan 4. Epub 2018 Jan 4.
    Department of Molecular Biotechnology and Bioinformatics, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla, 90112, Thailand.
    A Dot-blot immunogold assay (DBIA) was developed to detect white spot syndrome virus (WSSV) using the polyclonal antibody VP26 (anti-VP26). The anti-VP26 was immobilized on gold nanoparticles (Ab-AuNPs) and a nitrocellulose membrane was used as a detection pad. When the target WSSV bound to the Ab-AuNPs a reddish dots appeared on the surface of the membrane used within 2 to 5 minutes which could be seen with the naked eye. Read More

    In situ downstream strategies for cost-effective bio/surfactant recovery.
    Biotechnol Appl Biochem 2018 Jan 3. Epub 2018 Jan 3.
    Faculty of Life and Health Sciences, School of Biomedical Sciences, University of Ulster, Coleraine, N. Ireland.
    Since 60-80% of total costs of production are usually associated with downstream collection, separation and purification processes, it has become advantageous to investigate how to replace traditional methods with efficient and cost-effective alternative techniques for recovery and purification of biosurfactants. In the traditional techniques, large volumes of organic solvents are usually used increasing production cost and the overall environmental burden. In addition, traditional production and separation methods typically carried out in batch cultures reduce biosurfactant yields due to product inhibition and lower biosurfactants activity as a result of interaction with the organic solvents used. Read More

    Improved pulp bleaching potential of Bacillus subtilis WB800 through overexpression of three lignolytic enzymes from various bacteria.
    Biotechnol Appl Biochem 2017 Dec 29. Epub 2017 Dec 29.
    Department of Biology, Faculty of Science, Karadeniz Technical University, Trabzon, Turkey.
    A chemical bleaching process of paper pulps gives off excessive amount of chlorinated organic wastes mostly released to environment without exposing complete bioremediaton. Recent alternative and eco-friendly approaches toward pulp bleaching appear more responsive to environmental awareness. Here we report, direct use of a recombinant Bacillus subtilis bacterium for pulp bleaching, endowed with three ligninolytic enzymes from various bacteria. Read More

    Human neonatal Fc receptor as a new potential antibody binding protein for antibody immobilization.
    Biotechnol Appl Biochem 2017 Dec 27. Epub 2017 Dec 27.
    Institute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia, Pulau Pinang, Malaysia.
    A critical challenge in producing an antibody-based assay with the highest reproducibility and sensitivity is the strategy to immobilize antibodies to solid phase. To date, numerous methods of antibody immobilization were reported but each was subjected to its advantages and limitations. The current study proposes a new potential antibody binding protein, the human neonatal fragment crystallizable (Fc) receptor. Read More

    Investigation of autophagy and differentiation of myenteric interstitial cells of cajal in the pathogenesis of gastric motility disorders in rats with functional dyspepsia.
    Biotechnol Appl Biochem 2017 Dec 23. Epub 2017 Dec 23.
    The First Affiliated Hospital of Guangxi Medical University, Nanning, China.
    Background: Interstitial cells of Cajal (ICC), especially myenteric interstitial cells of Cajal (ICC-MY), are key to gastrointestinal motility. However, their role in the pathogenesis of functional dyspepsia (FD) is unclear. Therefore, autophagy and differentiation of ICC-MY were investigated to elucidate the pathogenesis of gastric motility disorder in FD. Read More

    Effects of celastrol on Tau hyperphosphorylation and expression of HSF-1 and HSP70 in SH-SY5Y neuroblastoma cells induced by amyloid-β peptides.
    Biotechnol Appl Biochem 2017 Dec 22. Epub 2017 Dec 22.
    Department of Clinical Lab, Shanghai Gongli Hospital, the Second Military Medical University, Pudong New District, Shanghai, People's Republic of China.
    To observe the effects of celastrol on Tau hyperphosphorylation induced by amyloid-β peptides (Aβ) in SH-SY5Y neuroblastoma cells, the changes of Tau hyperphosphorylation and the expression of heat shock protein 90 (HSP90), HSP70, and heat shock factor 1 (HSF-1) in SH-SY5Y cells treated with Aβ1-42 and celastrol were measured. Tau hyperphosphorylation and HSP90 expression induced by Aβ1-42 was also measured by Western blotting after HSP70 or HSF-1 knockdown by siRNA. The interaction between HSP70 and Tau or HSP70 and carboxyl terminus of HSP70 interacting protein (CHIP) was measured by co-immunoprecipitation. Read More

    Peptide (Lys-Leu) and amino acids (Lys and Leu) supplementations improve physiological activity and fermentation performance of brewer's yeast during very high-gravity (VHG) wort fermentation.
    Biotechnol Appl Biochem 2017 Dec 22. Epub 2017 Dec 22.
    School of Food Science and Engineering, South China University of Technology, Guangzhou, People's Republic of China.
    Lys and Leu were generally considered as the key amino acids for brewer's yeast during beer brewing. In the present study, peptide Lys-Leu and a free amino acid (FAA) mixture of Lys and Leu (Lys + Leu) were supplemented in 24 °P wort to examine their effects on physiological activity and fermentation performance of brewer's yeast during very high-gravity (VHG) wort fermentation. Results showed that although both peptide Lys-Leu and their FAA mixture supplementations could increase the growth and viability, intracellular trehalose and glycerol content, wort fermentability, and ethanol content for brewer's yeast during VHG wort fermentation, and peptide was better than their FAA mixture at promoting growth and fermentation for brewer's yeast when the same dose was kept. Read More

    Noncoded amino acids in protein engineering: Structure-activity relationship studies of hirudin-thrombin interaction.
    Biotechnol Appl Biochem 2017 Dec 12. Epub 2017 Dec 12.
    Laboratory of Protein Chemistry, Department of Pharmaceutical & Pharmacological Sciences, University of Padua, Padua, Italy.
    The advent of recombinant DNA technology allowed to site-specifically insert, delete, or mutate almost any amino acid in a given protein, significantly improving our knowledge of protein structure, stability, and function. Nevertheless, a quantitative description of the physical and chemical basis that makes a polypeptide chain to efficiently fold into a stable and functionally active conformation is still elusive. This mainly originates from the fact that nature combined, in a yet unknown manner, different properties (i. Read More

    Immobilization of carbonic anhydrase on polyvinylidene fluoride membranes.
    Biotechnol Appl Biochem 2017 Dec 9. Epub 2017 Dec 9.
    School of Chemistry and Chemical Engineering, Beijing Institute of Technology, Beijing, People's Republic of China.
    In recent years, the application of carbonic anhydrase (CA) in CO2 removal has attracted great interest. However, obtaining high enzyme recovery activity is difficult in existing immobilization techniques. In this work, water plasma-treated poly(vinylidene fluoride) (PVDF) membranes were modified via 3-aminopropyl triethoxy silane (KH550) or γ-(2, 3-epoxypropoxy) propyl trimethoxy silane (KH560), and then CA was attached. Read More

    Application of aqueous two-phase micellar system to improve extraction of adenoviral particles from cell lysate.
    Biotechnol Appl Biochem 2017 Dec 4. Epub 2017 Dec 4.
    Department of Biochemical and Pharmaceutical Technology, School of Pharmaceutical Sciences, University of São Paulo, São Paulo, Brazil.
    Viral vectors are important in medical approaches, such as disease prevention and gene therapy, and their production depends on efficient prepurification steps. In the present study, an aqueous two-phase micellar system (ATPMS) was evaluated to extract human adenovirus type 5 particles from a cell lysate. Adenovirus was cultured in human embryonic kidney 293 (HEK-293) cells to a concentration of 1. Read More

    Novel antioxidant and anti-inflammatory peptides from the Siamese crocodile (Crocodylus siamensis) hemoglobin hydrolysate.
    Biotechnol Appl Biochem 2017 Nov 22. Epub 2017 Nov 22.
    Protein and Proteomics Research Center for Commercial and Industrial Purposes (ProCCI), Faculty of Science, Khon Kaen University, Khon Kaen, Thailand.
    Novel antioxidant and anti-inflammatory peptides were isolated from hydrolysates of Siamese crocodile (Crocodylus siamensis) hemoglobin. C. siamensis hemoglobin hydrolysates (CHHs) were obtained by pepsin digestion at different incubation times (2, 4, 6, and 8 H) at 37 °C and subjected to antioxidant and anti-inflammatory activity assessment. Read More

    Structure-based analysis of Clot as a thioredoxin-related protein of 14 kDa in Drosophila via experimental and computational approaches.
    Biotechnol Appl Biochem 2017 Oct 28. Epub 2017 Oct 28.
    Department of Medical Biotechnology, Soonchunhyang University, Asan, 31538, Korea.
    The clot gene is required for the biosynthesis of drosopterins, the red components of Drosophila eye pigments. However, the enzymatic role of Clot in Drosophila eye pigment formation and the molecular mechanisms underlying Clot function are not fully elucidated. In this study, we cloned and characterized Clot derived from Drosophila cDNA, and results showed that Clot exhibited ∼30% sequence identity with mammalian TRP14. Read More

    Large-scale purification of high purity α1-antitrypsin from Cohn Fraction IV with virus inactivation by solvent/detergent and dry-heat treatment.
    Biotechnol Appl Biochem 2017 Oct 26. Epub 2017 Oct 26.
    Beijing Key Laboratory of Blood Safety and Supply Technologies, Beijing Institute of Transfusion Medicine, Beijing, People's Republic of China.
    α1-Antitrypsin (AAT) is widely used to treat patients with congenital AAT deficiency. Cohn Fraction IV (Cohn F IV) is normally discarded during the manufacturing process of albumin but contains approximately 33% of plasma AAT. We established a new process for large-scale purification of AAT from it. Read More

    Impact of mycolic acid deficiency on cells of Corynebacterium glutamicum ATCC13869.
    Biotechnol Appl Biochem 2017 Oct 26. Epub 2017 Oct 26.
    School of Biotechnology, Jiangnan University, Wuxi, People's Republic of China.
    Mycolic acid (MA) plays important role in Corynebacterium glutamicum, but the key enzymes in the biosynthetic pathway of MA in C. glutamicum ATCC13869 have not been characterized. Since the locus BBD29_RS14045 in C. Read More

    Trans-acting oligodeoxythymidine phosphorothioate triester reagents for transient transfection optimized and facilitated by a high-throughput microbioreactor system.
    Biotechnol Appl Biochem 2017 Oct 10. Epub 2017 Oct 10.
    Division of Biotechnology Review and Research II, OBP, CDER, Food and Drug Administration, Silver Spring, MD, USA.
    A rapid and cost-effective transient transfection method for mammalian cells is essential for screening biopharmaceuticals in early stages of development. A library of 25 amphipathic trans-acting oligodeoxythymidine phosphorothioate triester (dTtaPS) transfection reagents, carrying positively charged and lipophilic groups, has been constructed for this purpose. High-throughput screening of the library, using an imaging cytometer and an automated microbioreactor system, has led to the identification of dTtaPS10+ as a potent transfection reagent. Read More

    Biosensors: Classifications, medical applications, and future prospective.
    Biotechnol Appl Biochem 2017 Oct 10. Epub 2017 Oct 10.
    Faculty of Applied Medical Sciences, Department of Medical Laboratory Technology, King Abdulaziz University, Jeddah, Kingdom of Saudi Arabia.
    Biosensors are devices that combine a biological material with a suitable platform for detection of pathogenic organisms, carcinogenic, mutagenic, and/or toxic chemicals or for reporting a biological effect. In recent years, an enormous number of different types of biosensors have been constructed and developed for several medical applications. The reason for that was primarily due to the numerous advantages and applications that can be offered by biosensors. Read More

    The BMP antagonist Noggin is produced by osteoblasts in response to the presence of prostate cancer cells.
    Biotechnol Appl Biochem 2017 Oct 5. Epub 2017 Oct 5.
    Department of Oncology and Metabolism, Medical School, University of Sheffield, Sheffield, United Kingdom.
    Bone metastasis is a key event responsible for morbidity in prostate cancer patients. Interactions between prostate cancer cells and the bone microenvironment facilitate survival of tumor cells and alter bone turnover, a process that is thought to enhance the growth of metastases in this site. This study aimed to test the hypothesis that the presence of tumors cells increases transforming growth factor beta (TGF-β) signaling in bone and that this regulates the proliferation and differentiation of osteoblastic lineage cells in metastatic sites. Read More

    Construction of tissue-engineered lymphatic vessel using human adipose derived stem cells differentiated lymphatic endothelial like cells and decellularized arterial scaffold: A preliminary study.
    Biotechnol Appl Biochem 2017 Oct 5. Epub 2017 Oct 5.
    Department of Microsurgery and Orthopedic Trauma, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, People's Republic of China.
    We have previously demonstrated that human adipose-derived stem cells (hADSCs) can be differentiated into lymphatic endothelial like cells. The purpose of this study was to investigate the feasibility of utilizing the induced lymphatic endothelial like cells and decellularized arterial scaffold to construct the tissue-engineered lymphatic vessel. The hADSCs were isolated from adipose tissue in healthy adults and were characterized the multilineage differentiation potential. Read More

    Progress in biopharmaceutical development.
    Biotechnol Appl Biochem 2017 Oct 3. Epub 2017 Oct 3.
    Department of Bioengineering, Institute of Biotechnology and Antibiotics, Warsaw, Poland.
    Since its introduction in 1982, biopharmaceutical drugs have revolutionized the treatment of a broad spectrum of diseases and are increasingly used in nearly all branches of medicine. In recent years, the biopharmaceuticals market has developed much faster than the market for all drugs and is believed to have great potential for further dynamic growth because of the tremendous demand for these drugs. Biobetters, which contain altered active pharmaceutical ingredients with enhanced efficacy, will play an important role in the development of biopharmaceuticals. Read More

    FleQ regulates both the type VI secretion system and flagella in Pseudomonas putida.
    Biotechnol Appl Biochem 2017 Oct 2. Epub 2017 Oct 2.
    State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, People's Republic of China.
    Based on the analysis of sodium dodecyl sulfate-polyacrylamide gel electrophoresis and mass spectrometry, flagellin FliC and a key member of the type VI secretion system Hcp1 were found to be secreted from Pseudomonas putida KT2442 cells in the late exponential phase, but in the early exponential phase only FliC was secreted. Relevant genes fleQ and rpoN were then individually deleted in P. putida KT2442, and the expression and transcription of fliC and hcp1 in these mutant strains were analyzed. Read More

    Studying the TRAF2 binding to model membranes: The role of subunits dissociation.
    Biotechnol Appl Biochem 2017 Sep 28. Epub 2017 Sep 28.
    Department of Experimental Medicine and Surgery, University of Rome Tor Vergata, Via Montpellier 1, 00133, Rome, Italy.
    The ability of a C-terminal truncated form of TRAF2 to bind synthetic vesicles has been quantitatively studied by steady-state fluorescence energy transfer from the protein to large unilamellar vesicles (LUVs) prepared with different lipid mixtures. The dissociation constants, the free energy of binding and the average number of phospholipids interacting with truncated-TRAF2 have been evaluated from the corresponding binding curves. The results indicate that the protein strongly interacts with the lipid bilayer, preferentially in the monomeric state. Read More

    Amine oxidase from Euphorbia characias: Kinetic and structural characterization.
    Biotechnol Appl Biochem 2017 Sep 20. Epub 2017 Sep 20.
    Department of Sciences of Life and Environment, University of Cagliari, Monserrato, Cagliari, Italy.
    This minireview focuses on a plant copper/2,4,5-trihydroxyphenyl alanine quinone amine oxidase isolated from the latex of the shrub Euphorbia characias (ELAO). This enzyme has been investigated in terms of both molecular structure and kinetic mechanism. The characterization of this enzyme allowed us to identify specific amino acids and domains that play a key role in modulating substrate access into the active site not only for ELAO but also for other plant and mammalian amine oxidases. Read More

    Working at the membrane interface: Ligand-induced changes in dynamic conformation and oligomeric structure in human aromatase.
    Biotechnol Appl Biochem 2017 Sep 19. Epub 2017 Sep 19.
    Department of Life Sciences and Systems Biology, University of Torino, Torino, Italy.
    Aromatase catalyzes the biosynthesis of estrogens from androgens. Owing to the physiological importance of this conversion of lipophilic substrates, the interaction with the lipid bilayer for this cytochrome P450 is crucial for its dynamics that must allow an easy access to substrates and inhibitors. Here, the aromatase-anastrozole interaction is studied by combining computational methods to identify possible access/egress routes with the protein inserted in the membrane and experimental tools aimed at the investigation of the effect of the inhibitor on the protein conformation. Read More

    Construction and application of an electrochemical biosensor based on an endotoxin aptamer.
    Biotechnol Appl Biochem 2017 Sep 9. Epub 2017 Sep 9.
    College of Pharmaceutical Science, ZheJiang University, HangZhou, People's Republic of China.
    An electrochemical biosensor that used an aptamer as a biological element was constructed to detect endotoxin. Biolayer interferometry was used to obtain the affinity constant of an aptamer for lipopolysaccharide, which had an equilibrium dissociation constant of 22.9 nM. Read More

    Improving properties of silk sericin membranes via enzymatic oxidation with laccase and TEMPO.
    Biotechnol Appl Biochem 2017 Sep 7. Epub 2017 Sep 7.
    Key Laboratory of Science and Technology of Eco-Textile, Ministry of Education, Jiangnan University, Wuxi, People's Republic of China.
    Silk sericin has excellent features of antioxidant ability and good cytocompatibility; however, high water solubility and poor mechanical properties have restricted its application in biomedical fields. In this study, aimed at improving the mechanical properties of a regenerated silk sericin, the primary hydroxy groups in silk sericin were enzymatically oxidized by using laccase and 2,2',6,6'-tetramethylpiperidine-N-oxyl (TEMPO), and the generated reactive groups then reacted further with the amino groups in the sericin chains. The efficacy of the enzymatic cross-linking was evaluated by means of determination of amino groups, SDS polyacrylamide gel electrophoresis, Fourier transform infrared (FTIR) spectroscopy, and size exclusion chromatography. Read More

    An activity transition from NADH dehydrogenase to NADH oxidase during protein denaturation.
    Biotechnol Appl Biochem 2017 Sep 7. Epub 2017 Sep 7.
    Department of Chemistry, Rutgers University-Camden, Camden, NJ, USA.
    A decrease in the specific activity of an enzyme is commonly observed when the enzyme is inappropriately handled or is stored over an extended period. Here, we reported a functional transition of an FMN-bound diaphorase (FMN-DI) that happened during the long-term storage process. It was found that FMN-DI did not simply lose its β-nicotinamide adenine diphosphate (NADH) dehydrogenase activity after a long-time storage, but obtained a new enzyme activity of NADH oxidase. Read More

    Membrane cholesterol effect on the 5-HT2A receptor: Insights into the lipid-induced modulation of an antipsychotic drug target.
    Biotechnol Appl Biochem 2017 Sep 6. Epub 2017 Sep 6.
    Research Programme on Biomedical Informatics (GRIB), Department of Experimental and Health Sciences of Pompeu Fabra University (UPF)-Hospital del Mar Medical Research Institute (IMIM), Barcelona, Spain.
    The serotonin 5-hydroxytryptamine 2A (5-HT2A ) receptor is a G-protein-coupled receptor (GPCR) relevant for the treatment of CNS disorders. In this regard, neuronal membrane composition in the brain plays a crucial role in the modulation of the receptor functioning. Since cholesterol is an essential component of neuronal membranes, we have studied its effect on the 5-HT2A receptor dynamics through all-atom MD simulations. Read More

    Taxadiene-5α-ol is a minor product of CYP725A4 when expressed in Escherichia coli.
    Biotechnol Appl Biochem 2017 Sep 6. Epub 2017 Sep 6.
    Department of Plant Biology, Southern Illinois University, Carbondale, IL, USA.
    CYP725A4 is a P450 enzyme from Taxus cuspidata that catalyzes the formation of taxadiene-5α-ol (T5α-ol) from taxadiene in paclitaxel biosynthesis. Past attempts expressing CYP725A4 in heterologous hosts reported the formation of 5(12)-oxa-3(11)-cyclotaxane (OCT) and/or 5(11)-oxa-3(11)-cyclotaxane (iso-OCT) instead of, or in addition to, T5α-ol. Here, we report that T5α-ol is produced as a minor product by Escherichia coli expressing both taxadiene synthase and CYP725A4. Read More

    Enhancement of  l-phenylalanine production in Escherichia coli by heterologous expression of Vitreoscilla hemoglobin.
    Biotechnol Appl Biochem 2017 Sep 5. Epub 2017 Sep 5.
    Engineering Research Center of Industrial Microbiology, Ministry of Education, Fujian Normal University, Fuzhou, People's Republic of China.
    l-Phenylalanine is an important amino acid that is widely used in the production of food flavors and pharmaceuticals. Generally, l-phenylalanine production by engineered Escherichia coli requires a high rate of oxygen supply. However, the coexpression of Vitreoscilla hemoglobin gene (vgb), driven bya tac promoter, with the genes encoding 3-deoxy-d-arabinoheptulosonate-7-phosphate synthetase (aroF) and feedback-resistant chorismate mutase/prephenate dehydratase (pheAfbr ), led to increased productivity and decreased demand for aeration by E. Read More

    Size of single-wall carbon nanotube affects the folate receptor-mediated cancer cell targeting.
    Biotechnol Appl Biochem 2017 Aug 30. Epub 2017 Aug 30.
    Department of Nanobiotechnology, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran.
    Advances in nanobiotechnology and targeting strategy could improve the delivery of therapeutic molecules into cancer cells, leading to improved treatment efficiency with minimal side effects on normal cells. To design an efficient nanocarrier, consideration of parameters that facilitate direct drug delivery into the target cells is important. We studied the effect of single-wall carbon nanotubes (SWNTs) size on their cell internalization level via the folate receptor-mediated pathway through folic acid targeting. Read More

    A camelid nanobody against EGFR was easily obtained through refolding of inclusion body expressed in Escherichia coli.
    Biotechnol Appl Biochem 2017 Nov 29;64(6):895-901. Epub 2017 Aug 29.
    School of Life Science and Biotechnology, Dalian University of Technology, Dalian City, Liaoning Province, People's Republic of China.
    Using anti-EGFR (epidermal growth factor receptor) nanobody is a good choice for diagnoses and therapeutics for high EGFR expression diseases. In the present study, the percentage composition of anti-EGFR nanobody attained 25% of the total cell protein expressed in Escherichia coli BL21 (DE3). However, almost all nanobodies were expressed as inclusion bodies. Read More

    Enhancing recombinant interleukin-6 production yield by fermentation optimization, two-step denaturing, and one-step purification.
    Biotechnol Appl Biochem 2017 Aug 22. Epub 2017 Aug 22.
    National Centre of Excellence in Molecular Biology, University of the Punjab, Lahore, Pakistan.
    Interleukin-6 a pleiotropic cytokine involved in a wide range of biological activities. So the large-scale production of biologically active recombinant human interleukin-6 is important for its structural and functional studies. Here, we report an optimized method for shake flask fermentation and a simplified high-yield purification procedure for the recombinant interleukin-6. Read More

    Generation and selection of naïve Fab library for parasitic antigen: Anti-BmSXP antibodies for lymphatic filariasis.
    Biotechnol Appl Biochem 2017 Aug 21. Epub 2017 Aug 21.
    Institute for Research in Molecular Medicine, Universiti Sains Malaysia, Minden, Penang, Malaysia.
    Phage display has been applied successfully as a tool for the generation of monoclonal antibodies (mAbs). Naive antibody libraries are unique as they are able to overcome several limitations associated with conventional mAb generation methods like the hybridoma technology. Here, we performed an in vitro selection and generation of Fab antibodies against Brugia malayi SXP protein (BmSXP), a recombinant antigen for the detection of lymphatic filariasis. Read More

    Microbial production of mannitol by Lactobacillus brevis 3-A5 from concentrated extract of Jerusalem artichoke tubers.
    Biotechnol Appl Biochem 2017 Aug 17. Epub 2017 Aug 17.
    Dalian Institute of Chemical Physics, Chinese Academy of Sciences, CAS, Dalian, People's Republic of China.
    In the present study, the conversion of the extract of Jerusalem artichoke tubers for mannitol production by Lactobacillus brevis 3-A5 was investigated. When the bacterium utilized enzymatic hydrolysates of Jerusalem artichoke extract as the main substrates in batch fermentation, the significant decrease in mannitol productivity was observed when the initial concentration of reducing sugar increased. Then, a strategy of continuous fed-batch fermentation was adopted for improving mannitol production with enzymatic hydrolysates of Jerusalem artichoke extract as main substrates. Read More

    In silico mapping of allosteric ligand binding sites in type-1 cannabinoid receptor.
    Biotechnol Appl Biochem 2017 Aug 17. Epub 2017 Aug 17.
    European Center for Brain Research (CERC)/Santa Lucia Foundation IRCCS, Rome, Italy.
    The recent resolution of the crystal structure of type-1 cannabinoid receptor (CB1 ) and the discovery of novel modulators for this target open the way to the possibility of elucidating the structural requirements for CB1 binding, and thereby facilitate a rational drug design. Compounds that target the orthosteric site of CB1 in some cases have shown side effects. Allosteric modulators could potentially avoid these side effects by influencing binding and/or efficacy of orthosteric ligands. Read More

    Cloning and bacterial expression systems for recombinant human heparanase production: Substrate specificity investigation by docking of a putative heparanase substrate.
    Biotechnol Appl Biochem 2017 Aug 14. Epub 2017 Aug 14.
    Institute of Food Science, CNR, Avellino, Italy.
    Human heparanase (HPSE) is an enzyme that degrades the extracellular matrix. It is implicated in a multiplicity of physiological and pathological processes encouraging angiogenesis and tumor metastasis. The protein is a heterodimer composed of a subunit of 8 kDa and another of 50 kDa. Read More

    Prediction of shorter oligonucleotide sequences recognizing aflatoxin M1.
    Biotechnol Appl Biochem 2017 Aug 10. Epub 2017 Aug 10.
    Dairy Chemistry Division, National Dairy Research Institute, Karnal, Haryana, India.
    Aflatoxin M1 (AFM1) is present in milk of lactating animals fed on aflatoxin B1 contaminated feeds. Aptamers are new emerging ligand molecules and can be employed in assay protocols. Shorter aptamers have several advantages. Read More

    Effects of gelling bath on the physical properties of alginate gel beads and the biological characteristics of entrapped HepG2 cells.
    Biotechnol Appl Biochem 2017 Aug 9. Epub 2017 Aug 9.
    Scientific Research Center for Translational Medicine, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, People's Republic of China.
    Optimizing alginate gel beads is necessary to support the survival, proliferation, and function of entrapped hepatocytes. In this study, gelling bath was modified by decreasing calcium ion concentration and increasing sodium ion concentration. Alginate gel beads (using 36% G sodium alginate) prepared in the modified gelling bath had more homogeneous structure and better mass transfer properties compared with the traditional gelling bath that contains only calcium ions. Read More

    Molecular dynamics study on the Apo- and Holo-forms of 5-lipoxygenase.
    Biotechnol Appl Biochem 2017 Aug 8. Epub 2017 Aug 8.
    European Center for Brain Research (CERC)/Santa Lucia Foundation IRCCS, Rome, Italy.
    Lipoxygenases (LOXs) are nonheme iron-containing enzymes catalyzing the dioxygenation of polyunsaturated fatty acids. LOX catalytic activity depends on the presence of iron in the active site and the iron removal is also able to affect the membrane binding properties of the enzyme. Leukotrienes biosynthesis is initiated by the action of 5-LOX at the level of nuclear membrane and the mechanism of enzyme-membrane interaction is thought to involve structural flexibility and conformational changes at the level of the protein tertiary structure. Read More

    Metformin promotes neuronal differentiation and neurite outgrowth through AMPK activation in human bone marrow-mesenchymal stem cells.
    Biotechnol Appl Biochem 2017 Nov 7;64(6):836-842. Epub 2017 Sep 7.
    Department of Biology, College of Natural Science, Chosun University, Gwangju, Korea.
    Metformin is an AMP-activated kinase (AMPK) activator that plays a role in glucose energy metabolism and cell protection. It is widely used to treat several diseases, including type 2 diabetes, cardiovascular diseases, cancer, and metabolic diseases. In this study, we investigated whether AMPK activation upon treatment with metformin may promote neurite outgrowth during the progression of neuronal differentiation in human bone marrow-mesenchymal stem cells (hBM-MSCs). Read More

    Construct a gene-to-metabolite network to screen the key genes of triterpene saponin biosynthetic pathway in Panax notoginseng.
    Biotechnol Appl Biochem 2017 Aug 4. Epub 2017 Aug 4.
    State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, 210009, People's Republic of China.
    Triterpene saponins are main active constituents of Panax notoginseng. Metabolites profiling of 12 triterpene saponins was analyzed by high-performance liquid chromatography-mass spectrometry in leaf, petiole, and root extracts of P. notoginseng. Read More

    SEC-SAXS and HDX-MS: A powerful combination. The case of the calcium-binding domain of a bacterial toxin.
    Biotechnol Appl Biochem 2017 Aug 2. Epub 2017 Aug 2.
    Institut de Biologie Intégrative de la Cellule, UMR 9198, Université Paris-Sud, Orsay, France.
    Small-angle X-ray scattering (SAXS) is a relatively simple experimental technique that provides information on the global conformation of macromolecules in solution, be they fully structured, partially, or extensively unfolded. Size exclusion chromatography in line with a SAXS measuring cell considerably improves the monodispersity and ideality of solutions, the two main requirements of a "good" SAXS sample. Hydrogen/deuterium exchange monitored by mass spectrometry (HDX-MS) offers a wealth of information regarding the solvent accessibility at the local (peptide) level. Read More

    Co-expression of alpha-1 antitrypsin with cytoplasmic domain of v-SNARE in Pichia pastoris: Preserving biological activity of alpha-1 antitrypsin.
    Biotechnol Appl Biochem 2017 Aug 1. Epub 2017 Aug 1.
    Department of Biochemistry, Faculty of Basic Science, Tarbiat Modares University, Tehran, Iran.
    Alpha-1-antitrypsin (A1AT) is a major serum protein in human with protease inhibitory activity. Because of its extensive application in medicine, recombinant DNA technology has been considered for its production. The current study examines coexpression of A1AT and soluble domain of v-SNARE in Pichia pastoris, which can prevent the secretion of A1AT after thoroughly passing the secretory pathway. Read More

    Improvement of amphotericin B production by a newly isolated Streptomyces nodosus mutant.
    Biotechnol Appl Biochem 2017 Aug 1. Epub 2017 Aug 1.
    Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, 310014, People's Republic of China.
    Amphotericin B is an effective antifungal antibiotic. However, its production in the wild-type strain of Streptomyces nodosus is relatively low. In this study, a strain of ZJB 20130827, capable of producing amphotericin B, was isolated and identified as S. Read More

    Role of Q177A and K173A/Q177A substitutions in thermostability and activity of the ELBn12 lipase.
    Biotechnol Appl Biochem 2017 Jul 19. Epub 2017 Jul 19.
    Department of Industrial and Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran.
    Thermostable lipases have many applications in detergent industries and in organic synthesis. There are many ways to improve thermal stability of enzymes, for example, higher hydrophobicity, greater structural packing, higher content of the charged residues, and lower thermolabile ones. In this study, thermolabile Gln (sensitive to higher temperatures) was substituted with Ala in native ELBn12 and mutated K173A lipases to examine its effect on thermal stability and activity of the lipases. Read More

    Role of palmitoylation of cysteine 415 in functional coupling CB1 receptor to Gαi2 protein.
    Biotechnol Appl Biochem 2017 Jul 19. Epub 2017 Jul 19.
    Faculty of Bioscience and Technology for Food Agriculture and Environment, University of Teramo, Teramo, Italy.
    In this study, we investigated the role of CB1 palmitoylation in modulating the functional interaction with G proteins both in the absence and presence of agonist binding. Our data show that the nonpalmitoylated CB1 receptor significantly reduced its association with Gαi2 . The agonist stimulation induced a partial dissociation of Gαi2 proteins from the wild-type receptor, while on the C415A mutant the agonist binding was not able to induce a significant dissociation of Gαi2 from the receptor. Read More

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