2,129 results match your criteria Biotechnology and Applied Biochemistry[Journal]


Production of inulin- and neolevan-type fructooligosaccharides by Penicillium janczewskii Zaleski CCIBt 3352.

Biotechnol Appl Biochem 2019 Feb 13. Epub 2019 Feb 13.

Universidade Federal do ABC, Avenida dos Estados, 5001, CEP 09210-580, Santo André, SP, Brazil.

Fructooligosaccharides (FOS) are fructose-based oligosaccharides employed as additives to improve the nutritional and technological properties of foods. The rhizosphere of inulin-accumulating plants from the Cerrado (Brazilian savanna) harbor fungi capable of synthesizing FOS from sucrose through the transfructosylating activity of β-fructosyltransferases and/or β-fructofuranosidases. Here, we investigated the ability of Penicillium janczewskii Zaleski CCIBt 3352, a fungus isolated from the rhizosphere of Chrysolaena obovata (Asteraceae), to produce FOS in medium supplemented with sucrose concentrations of 30, 100 or 150 g L . Read More

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http://dx.doi.org/10.1002/bab.1738DOI Listing
February 2019

Selection and characterization of novel DNA aptamer against colorectal carcinoma Caco-2 cells.

Biotechnol Appl Biochem 2019 Feb 12. Epub 2019 Feb 12.

Department of Pharmacology, School of Medicine, Zhejiang University.

Aptamers are short, single-stranded nucleic acid (DNA or RNA) oligonucleotides that can be obtained by a technique called systematic evolution of ligands by exponential enrichment (SELEX) in vitro. Due to superior properties such as small size, high binding affinity and stability, they are considered to be feasible tools for diagnosis and treatment of disease. In the current study, we attempted to screen a high affinity DNA aptamer to selectively target the colorectal carcinoma Caco-2 cells by using cell-based SELEX approach. Read More

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http://dx.doi.org/10.1002/bab.1737DOI Listing
February 2019

Jasmonic acid-responsive AabHLH1 positively regulates artemisinin biosynthesis in Artemisia annua.

Biotechnol Appl Biochem 2019 Feb 4. Epub 2019 Feb 4.

Joint International Research Laboratory of Metabolic and Developmental Sciences, Key Laboratory of Urban Agriculture (South) Ministry of Agriculture, Plant Biotechnology Research Center, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, 200240, China.

Artemisia annua is the only natural source of the sesquiterpenoid artemisinin, which is widely used to treat malaria. The phytohormone jasmonic acid (JA) can significantly promote artemisinin biosynthesis in A. annua. Read More

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http://dx.doi.org/10.1002/bab.1733DOI Listing
February 2019
1.362 Impact Factor

High-proline proteins in experimental hazy white wine produced from partially botrytized grapes.

Biotechnol Appl Biochem 2019 Feb 4. Epub 2019 Feb 4.

Department of Protein Biochemistry and Proteomics, Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacký University, Olomouc, Czech Republic.

Undesirable effects of the pathogen Botrytis cinerea include reduced quality and quantity of wine grapes. Winemaking is also complicated by the formation of a protein haze in white wines and oxidative browning of red wines. We analyzed proteins in experimental Moravian white wines characterized by their instability and haze formation in bottles during storage despite prior bentonite treatment. Read More

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http://dx.doi.org/10.1002/bab.1736DOI Listing
February 2019

A novel strategy to synthesize dual-responsive polymeric nanocarriers for investigating the activity and stability of immobilized pectinase.

Biotechnol Appl Biochem 2019 Feb 4. Epub 2019 Feb 4.

Key Laboratory of Applied Surface and Colloid Chemistry, School of Chemistry & Chemical Engineering, Shaanxi Normal University, Xi'an, People's Republic of China.

A dual-stimuli-responsive support material for pectinase immobilization through ionic bonding was prepared. Specifically, polystyrene-b-polymethylacrylic (PS-b-PMAA), light- and pH-sensitive polystyrene-(5-propargylether-2-nitrobenzyl bromoisobutyrate)-b-poly(diethylamino)ethyl methacrylate-b-poly(polyethylene glycol methacrylate) (PS-ONB-PDEAEMA-b-PPEGMA) were synthesized through atom transfer radical polymerization, click chemistry, and hydrolysis. The two parts could self-assemble into the micelles in an aqueous solution. Read More

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http://dx.doi.org/10.1002/bab.1734DOI Listing
February 2019
1 Read

High cell density culture of baker's yeast FX-2 based on pH-stat coupling with respiratory quotient.

Biotechnol Appl Biochem 2019 Feb 4. Epub 2019 Feb 4.

Angel Yeast (Yili) Co., Ltd., Yining, China.

The high cell density culture of baker's yeast FX-2 was investigated in a 50 L(A) automatic bioreactor. Herein, it was found firstly that the Crabtree effect clearly existed in batch fermentation with higher glucose content, then the critical initial glucose content range (≤2.00 g L ) was reasonably ascertained to effectively avoid Crabtree effect. Read More

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http://dx.doi.org/10.1002/bab.1735DOI Listing
February 2019

Enzyme production by solid-state fermentation on soybean meal: A comparative study of conventional and ultrasound-assisted extraction methods.

Biotechnol Appl Biochem 2019 Jan 29. Epub 2019 Jan 29.

Faculty of Technology and Metallurgy, University of Belgrade, Department of Biochemical Engineering and Biotechnology, Belgrade, Serbia.

Protease, cellulase, and α-amylase producing Bacillus subtilis strain was cultivated by solid-state fermentation technique using soybean meal as a substrate. The aim of the present study was to establish a highly efficient enzymes' extraction method as a first stage in downstream processing. The conventional extraction procedure was optimized by determining pH, stirring rate, solid/liquid ratio, and time of extraction on enzymes' recoveries from fermented soybean meal. Read More

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http://dx.doi.org/10.1002/bab.1732DOI Listing
January 2019

Novel microfluidic graphene oxide-protein amperometric biosensor for detecting sulfur compounds.

Biotechnol Appl Biochem 2019 Jan 22. Epub 2019 Jan 22.

Department of Life Science Engineering, Faculty of New Science and Technology, University of Tehran, Tehran, Iran.

Sulfur compounds are essential for many industries and organisms; however, they cause serious respiratory problems in human beings. Therefore, determination of sulfur concentration is of paramount importance. The research approach in the field of detecting contaminants has led to smaller systems that provide faster and more effective ways for diagnosis purposes. Read More

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http://dx.doi.org/10.1002/bab.1731DOI Listing
January 2019
1.362 Impact Factor

Production and characterization of PHB-HV copolymer by Bacillus thuringiensis isolated from Eisenia foetida.

Biotechnol Appl Biochem 2019 Jan 17. Epub 2019 Jan 17.

N. Rama Varier Ayurveda Foundation, Madurai, India.

Polyhydroxyalkanoates (PHAs) are one of the most important biopolyesters produced by vast number of microorganisms that exist in the environment. PHAs gained much attention due to its inherent biodegradable and biocompatible ability along with its physicochemical properties, which are similar to those of conventional petroleum-based plastics. In the present study, PHA producing bacterial strains was isolated from earthworm. Read More

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http://dx.doi.org/10.1002/bab.1730DOI Listing
January 2019
1 Read

Modulation of callus growth and secondary metabolites in different Thymus species and Zataria multiflora micropropagated under ZnO nanoparticles stress.

Biotechnol Appl Biochem 2019 Jan 16. Epub 2019 Jan 16.

Department of Biological Sciences, National University of Medical Sciences, Rawalpindi, Pakistan.

Thymus species are aromatic plants with diverse applications in food industries and medicine. This study was conducted to evaluate the potential effect of ZnO nanoparticles (NPs) on callus proliferation and thymol and carvacrol production in three Thymus species, that is, T. vulgaris, T. Read More

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http://dx.doi.org/10.1002/bab.1727DOI Listing
January 2019
3 Reads

A novel screening method for potential naringinase-producing microorganisms.

Biotechnol Appl Biochem 2019 Jan 16. Epub 2019 Jan 16.

University Institute of Chemical Technology, Kavayitri Bahinabai Chaudhari North Maharashtra University, Jalgaon, Maharashtra, India.

Naringinase has high industrial importance, and the progress in naringinase research is still quite slow. The unavailability of an effective, simple screening method, which will be applicable to different microorganisms such as bacteria, fungi, and actinomycetes, is one of the main reasons for this gap. Therefore, a simple plate assay was developed for effective screening of microorganisms for naringinase by exposing to iodine vapors. Read More

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http://dx.doi.org/10.1002/bab.1728DOI Listing
January 2019
1 Read

Comparative detection method of early onset cytokine-induced stress in β-cells (INS-1E).

Biotechnol Appl Biochem 2019 Jan 15. Epub 2019 Jan 15.

Department of Biochemistry and Microbiology, Nelson Mandela University, Port Elizabeth, Eastern Cape, South Africa.

β-Cells contain a prominent endoplasmic reticulum (ER), disrupting ER homeostasis and function, activating the unfolded protein response (UPR). Currently, no direct protocols measure the UPR initiation. Current methods to measure ER stress include the quantification of nitric oxide (NO) (indirect method), Western blotting, and qRT-PCR of downstream components. Read More

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http://doi.wiley.com/10.1002/bab.1729
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http://dx.doi.org/10.1002/bab.1729DOI Listing
January 2019
3 Reads

Matrilin-2 prevents the TNFα-induced apoptosis of WB-F344 cells via suppressing JNK pathway.

Biotechnol Appl Biochem 2019 Jan 9. Epub 2019 Jan 9.

Department of Pathology, Weihai Municipal Hospital, Weihai, 264200, Shandong Province, China.

Oval cells, a kind of hepatic progenitor cell quiescent at normal condition, activates to proliferate and differentiate into hepatocytes under severe and long-term liver injury, which usually raises severe inflammation. However, how oval cell survives in the inflammatory milieu interne is still unclear. Tumor necrosis factor α (TNFα), mimicking inflammatory hepatic milieu interne, was used to treat oval cell line, WB-F344, to test the protective function of matrilin-2. Read More

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http://dx.doi.org/10.1002/bab.1726DOI Listing
January 2019
1 Read
1.362 Impact Factor

The role of gibberellin synthase gene GhGA2ox1 in upland cotton (Gossypium hirsutum L.) responses to drought and salt stress.

Biotechnol Appl Biochem 2019 Jan 8. Epub 2019 Jan 8.

State Key Laboratory of Cotton Biology, Institute of Cotton Research of Chinese Academy of Agricultural Sciences, Anyang, People's Republic of China.

Gibberellins (GAs) is one kind of important endogenous hormone in plants that regulates vegetative and reproductive growth of plants. GA2ox is a class of oxidase that plays a regulatory role in the third stage of GAs synthesis. In this paper, we cloned the GhGA2ox1 gene from an upland cotton (Gossypium hirsutum L. Read More

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http://doi.wiley.com/10.1002/bab.1725
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http://dx.doi.org/10.1002/bab.1725DOI Listing
January 2019
2 Reads

Preparation and anti-tumor efficiency evaluation of bacterial magnetosome-anti-4-1BB antibody complex: Bacterial magnetosome as antibody carriers isolated from Magnetospirillum gryphiswaldense.

Biotechnol Appl Biochem 2019 Jan 1. Epub 2019 Jan 1.

Department of Immunology, Cancer Institute and Cancer Hospital, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing, People's Republic of China.

Bacterial magnetosomes (BMs) are used as carriers for antibodies, enzymes, and nucleic acids. This study aimed to demonstrate the clinical utility of BMs derived from Magnetospirillum gryphiswaldense for use in anti-tumor immunotherapy. Bis(sulfosuccinimidyl) suberate (BS3) was used to prepare BM-anti-4-1BB antibody complexes. Read More

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http://dx.doi.org/10.1002/bab.1724DOI Listing
January 2019
1 Read

Production of a novel N-terminal PEGylated crisantaspase.

Biotechnol Appl Biochem 2018 Dec 29. Epub 2018 Dec 29.

Department of Biochemical and Pharmaceutical Technology, University of São Paulo, São Paulo, Brazil.

Crisantaspase is an asparaginase enzyme produced by Erwinia chrysanthemi and used to treat acute lymphoblastic leukemia (ALL) in case of hypersensitivity to Escherichia coli l-asparaginase (ASNase). The main disadvantages of crisantaspase are the short half-life (10 H) and immunogenicity. In this sense, its PEGylated form (PEG-crisantaspase) could not only reduce immunogenicity but also improve plasma half-life. Read More

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http://dx.doi.org/10.1002/bab.1723DOI Listing
December 2018
3 Reads

Cytoprotective, anti-inflammatory, and antioxidant properties of high-molecular-weight hyaluronan enriched with red orange extract in human fibroblasts exposed to ultra violet light B irradiation.

Biotechnol Appl Biochem 2018 Dec 27. Epub 2018 Dec 27.

PharmaChemistry Line, Istituto Italiano di Tecnologia, Genova, Italy.

Ultraviolet (UV) light exposure is the primary factor responsible for skin photoaging, affecting all the skin layers, mainly through the production of reactive oxygen species (ROS), activation of inflammatory responses, and apoptosis. In keeping with this evidence, exogenous supplementation with dietary antioxidants has been shown to provide photoprotective benefits. Moreover, oral administration of hyaluronic acid (HA) has been proved to reduce the signs of aged skin, such as wrinkles, and increase hydration and elasticity. Read More

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http://doi.wiley.com/10.1002/bab.1722
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http://dx.doi.org/10.1002/bab.1722DOI Listing
December 2018
11 Reads

Isolation and screening for limonin-producing endophytic bacteria from Citrus maxima (Burm.) Merr. cv. Shatian Yu.

Biotechnol Appl Biochem 2018 Dec 22. Epub 2018 Dec 22.

College of Life Science, Shanxi University, Taiyuan, China.

Limonin, a compound of highly oxidized triterpenoids, has potential functions in preventing or slowing the occurrences of many diseases. In this study, five different bacterial strains were isolated and identified from Citrus maxima (Burm.) Merr. Read More

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http://doi.wiley.com/10.1002/bab.1721
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http://dx.doi.org/10.1002/bab.1721DOI Listing
December 2018
2 Reads

Bioconversion of lignin and its derivatives into polyhydroxyalkanoates: Challenges and opportunities.

Biotechnol Appl Biochem 2018 Dec 20. Epub 2018 Dec 20.

Department of Chemical Engineering, Chungbuk National University, Chungbuk, Republic of Korea.

Renewable energy resources are considered to be promising for the development of a sustainable circular economy. Among various alternatives, the microbial route for various biofuels production is quite lucrative. Use of cellulose and lignocellulose for methane, H , organic acids, ethanol, and cellulase has been explored a lot in the past few decades. Read More

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http://dx.doi.org/10.1002/bab.1720DOI Listing
December 2018
1 Read
1.362 Impact Factor

Salidroside protected against MPP -induced Parkinson's disease in PC12 cells by inhibiting inflammation, oxidative stress and cell apoptosis.

Biotechnol Appl Biochem 2018 Dec 13. Epub 2018 Dec 13.

Department of Neurosurgery, First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China.

The present study aimed to investigate the protective effects of salidroside (SAL) on 1-methyl-4-phenylpyridinium (MPP )-induced PC12 cell model for Parkinson's disease. PC12 cells were pretreated with SAL in different concentrations and then exposed to MPP . To evaluate the effects of SAL on cytotoxicity, the survival rate was tested by the 3-(4,5-dimethylthiazol-2-yl)-2,5-dimethyltetrazolium bromide (MTT) assay and the apoptosis was tested via flow cytometry and Western blot. Read More

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http://dx.doi.org/10.1002/bab.1719DOI Listing
December 2018
8 Reads

Employment of fucosidases for the synthesis of fucosylated oligosaccharides with biological potential.

Biotechnol Appl Biochem 2018 Nov 22. Epub 2018 Nov 22.

Departamento de Biotecnología, Universidad Autónoma Metropolitana-Iztapalapa, Ciudad de México, México.

Fucosylated oligosaccharides play important physiological roles in humans, including in the immune response, transduction of signals, early embryogenesis and development, growth regulation, apoptosis, pathogen adhesion, and so on. Efforts have been made to synthesize fucosylated oligosaccharides, as it is difficult to purify them from their natural sources, such as human milk, epithelial tissue, blood, and so on. Within the strategies for its in vitro synthesis, it is remarkable the employment of fucosidases, enzymes that normally cleave the fucosyl residue from the non-reducing end of fucosylated compounds, as these enzymes are also capable of synthesizing them by means of a transfucosylation reaction. Read More

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http://dx.doi.org/10.1002/bab.1714DOI Listing
November 2018
2 Reads

SOX11 regulates apoptosis and cell cycle in hepatocellular carcinoma via Wnt/β-catenin signaling pathway.

Biotechnol Appl Biochem 2018 Dec 5. Epub 2018 Dec 5.

Department of Hepatobiliary Surgery, Affiliated Hospital of North Sichuan Medical College, Nanchong, People's Republic of China.

Hepatocellular carcinoma (HCC) is the most common type of liver cancer with high mortality. Identifying key molecules involved in the regulation of HCC development is of great clinical significance. SOX11 is a transcription factor belonging to group C of Sry-related high mobility group box family whose abnormal expression is frequently seen in many kinds of human cancers. Read More

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http://doi.wiley.com/10.1002/bab.1718
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http://dx.doi.org/10.1002/bab.1718DOI Listing
December 2018
7 Reads

Enhanced cecropin B2 production via chitin-binding domain and intein self-cleavage system.

Biotechnol Appl Biochem 2018 Nov 24. Epub 2018 Nov 24.

Department of Chemical Engineering, National Chung Hsing University, Taichung, Taiwan, Republic of China.

In this study, various constructs and hosts were used to produce high levels of cecropin B2 (cecB2). To mitigate cecB2's toxic inhibition of host cells, various cecB2 constructs were built. Results showed that the combination of a chitin-binding domain and an intein self-cleavage motif in front of cecropin B2, without a His-tag, was best for cecB2 expression. Read More

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http://dx.doi.org/10.1002/bab.1716DOI Listing
November 2018
7 Reads

Cai's Neiyi Prescription promotes apoptosis and inhibits inflammation in endometrial stromal cells with endometriosis through inhibiting USP10.

Biotechnol Appl Biochem 2018 Nov 23. Epub 2018 Nov 23.

Department of Traditional Chinese Medicine, Shanghai General Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, People's Republic of China.

To observe the effect of Cai's Neiyi Prescription (CNYP) on the apoptosis and inflammation in endometrial stromal cells with endometriosis (EM) both in vivo and in vitro, EM model rats and endometrial stromal cells were treated with CNYP and the level of USP10, p-ERK1/2, ERK1/2, and apoptosis-related protein as well as the levels of proinflammatory factors were measured by Western blotting and ELISA, respectively. Rats with surgically induced EM showed increased USP10 expression and ERK/2 activation. Intragastric administration of CNYP granule significantly inhibited EM-induced ERK1/2 activation and expression of USP10 and Bcl-2, but increased the expression of Bax and Caspase-7 in EM-induced rats. Read More

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http://dx.doi.org/10.1002/bab.1715DOI Listing
November 2018
6 Reads

Effective filter screening approach with fractionation for enabling inline filtration of protein A eluate.

Biotechnol Appl Biochem 2018 Nov 22. Epub 2018 Nov 22.

Purification Process Sciences, MedImmune LLC, One MedImmune Way, Gaithersburg, MD, USA.

Filtration of protein A eluates inline with a chromatography column is a common challenge for monoclonal antibody (mAb) purification due to the high system backpressure during elution, which can result in system shut down or require a decreased elution flow rate. The inability to filter inline not only poses a risk for process deviations, but can also lead to tank constraints and microbial ingress risk. Here, we evaluated and described a novel approach for identifying filters for inline filtration of protein A eluates at pilot scale. Read More

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http://dx.doi.org/10.1002/bab.1713DOI Listing
November 2018
1 Read
1.362 Impact Factor

Application of ultra-rapid qPCR and DNA chips for viral RNA detection and confirmation.

Biotechnol Appl Biochem 2018 Nov 19. Epub 2018 Nov 19.

Department of Life Science, Kyonggi University, Suwon, Republic of Korea.

The rapid and accurate detection of the presence of microorganisms, such as viruses, has been an important issue in the fields of public health, as well as agriculture. A PCR-based detection method has been developed and applied in these fields to determine the presence of specific pathogens. Although the major advantage of real-time PCR is the monitoring of amplification and ability to quantify the template genes, the method described here should solve the problem of nonspecific product synthesis. Read More

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http://doi.wiley.com/10.1002/bab.1711
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http://dx.doi.org/10.1002/bab.1711DOI Listing
November 2018
9 Reads

Effects of multicopper oxidase orientation in multiwalled carbon nanotube biocathodes on direct electron transfer.

Biotechnol Appl Biochem 2018 Nov 14. Epub 2018 Nov 14.

Department of Frontier Fiber Technology and Science, Graduate School of Engineering, University of Fukui, Fukui, Japan.

In this study, multicopper oxidase (MCO) was immobilized on multiwalled carbon nanotubes (MWCNTs) at two different orientations, and the electrochemical properties of the resulting cathodes were investigated. Using N- or C-terminal His-tagged MCO and MWCNTs, we constructed two types of cathodes. We assumed that the distance between the type 1 (T1)Cu of the C-terminal His-tagged MCO and the MWCNT surface was lesser than that between the T1Cu of the N-terminal His-tagged MCO and the MWCNT surface. Read More

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http://doi.wiley.com/10.1002/bab.1710
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http://dx.doi.org/10.1002/bab.1710DOI Listing
November 2018
2 Reads

Identification of the first Crocodylus siamensis cathelicidin gene and RN15 peptide derived from cathelin domain exhibiting antibacterial activity.

Biotechnol Appl Biochem 2018 Nov 10. Epub 2018 Nov 10.

Protein and Proteomics Research Center for Commercial and Industrial Purposes (ProCCI), Faculty of Science, Khon Kaen University, Khon Kaen, Thailand.

Cathelicidins are effector molecules of vertebrate immunity that play vital roles against microbial invasion. They are widely identified in mammals, but few have been reported in Crocodilians, which are considered to be species with a powerful immune system. In the present study, we identified and characterized a novel cathelicidin from the blood of the Siamese crocodile, Crocodylus siamensis. Read More

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http://doi.wiley.com/10.1002/bab.1709
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http://dx.doi.org/10.1002/bab.1709DOI Listing
November 2018
9 Reads

Circulating miR-26a and miR-21 as biomarkers for glioblastoma multiform.

Biotechnol Appl Biochem 2018 Nov 8. Epub 2018 Nov 8.

Department of Biochemistry, Pasteur Institute of Iran, Tehran, Iran.

Glioblastoma multiform is the most common and lethal primary central nervous system tumor. Circulating microRNAs (miRNAs), present in cell-free bodily fluids, have been gaining importance as cancer biomarkers. The primary aim of this study was to assess whether circulating miRNA-128, -21, and -26a in glioblastoma patients can be used as diagnostic biomarkers. Read More

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http://doi.wiley.com/10.1002/bab.1707
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http://dx.doi.org/10.1002/bab.1707DOI Listing
November 2018
14 Reads

The C-terminal region of tumor necrosis factor like weak inducer of apoptosis is required for interaction with advanced glycation end products.

Biotechnol Appl Biochem 2018 Nov 7. Epub 2018 Nov 7.

Department of Pharmacology, School of Pharmacy, Shujitsu University, Okayama, Japan.

Previously, we found that endogenously produced pro-inflammatory molecules, advanced glycation end products (AGEs), interact with tumor necrosis factor-like weak inducer of apoptosis (TWEAK), and attenuate its immunomodulatory function. In the present study, to elucidate the mechanism by which AGEs attenuate TWEAK function, we searched for regions responsible for TWEAK-AGE interaction using TWEAK deletion mutants. Pull-down assays with the TWEAK mutants and AGEs revealed that the C-terminal half of TWEAK, which is the region essential for receptor stimulation, was required for this interaction. Read More

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http://dx.doi.org/10.1002/bab.1706DOI Listing
November 2018
7 Reads

Antioxidant and reactive oxygen species scavenging properties of cellular albumin in HepG2 cells is mediated by the glutathione redox system.

Biotechnol Appl Biochem 2018 Nov 7. Epub 2018 Nov 7.

Department of Hematology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.

This study was carried out to examine the role of intracellular albumin in the modulation of oxidative damage induced by glutathione modifiers in HepG2 cells. Also, the relationship of albumin synthesis with oxidative stress factors including antioxidants was studied. HepG2 cell culture was supplemented with glutathione modifiers; L-Buthionine-sulfoximine (BSO; 0. Read More

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http://dx.doi.org/10.1002/bab.1708DOI Listing
November 2018
2 Reads

Expression of the hybrid antimicrobial peptide lactoferrin-lysozyme in Pichia pastoris.

Biotechnol Appl Biochem 2018 Nov 1. Epub 2018 Nov 1.

Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, People's Republic of China.

The use of lactoferrin antimicrobial peptides and lysozymes as traditional antibiotic alternatives is suitable for solving drug residue and pathogen resistance. In this study, bovine lactoferrin (LfcinB) and human lysozyme (hLY) were combined through fusion expression in Pichia pastoris GS115 driven by constitutive GAP promoter. For neutralizing the toxic property of the antimicrobial peptide, anion antioxidant peptides from porcine myofibrillar protein and enzymatically hydrolyzed chicken egg white were fused to the hybrid antimicrobial peptide LfcinB-hLY. Read More

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http://dx.doi.org/10.1002/bab.1705DOI Listing
November 2018
2 Reads

Expression and production of recombinant scorpine as a potassium channel blocker protein in Escherichia coli.

Biotechnol Appl Biochem 2019 Jan 12;66(1):119-129. Epub 2018 Nov 12.

Department of Biotechnology, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Scorpine is a cationic protein from the venom of Pandinus imperator, belonging to potassium channel blocker family, which has been shown to have antibacterial, antiviral, and antiplasmodia activities. In the present study, a pET-44a vector containing scorpine synthetic gene with T7 Promoter (pET 44a-His6-Nus-His6-tev-scorpine) was transferred into Escherichia coli Rosetta-gami B (DE3) for soluble expression of the protein in the cytoplasm and its overproduction. After confirming recombinant scorpine peptide expression using SDS-PAGE and Western blot, augmentation of production was performed during two stages. Read More

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http://doi.wiley.com/10.1002/bab.1704
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http://dx.doi.org/10.1002/bab.1704DOI Listing
January 2019
15 Reads
1.362 Impact Factor

Production of high crystallinity type-I cellulose from Komagataeibacter hansenii JR-02 isolated from Kombucha tea.

Biotechnol Appl Biochem 2019 Jan 29;66(1):108-118. Epub 2018 Nov 29.

State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi University, Nanning, Guangxi, People's Republic of China.

In this study, a bacterial cellulose (BC) producing strain was isolated from Kombucha tea and identified as Komagataeibacter hansenii JR-02 by morphological, physiological, and biochemical characterization and 16S rRNA sequence. Then, the media components and culture conditions for BC production were optimized. Result showed that the highest BC yield was 3. Read More

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http://doi.wiley.com/10.1002/bab.1703
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http://dx.doi.org/10.1002/bab.1703DOI Listing
January 2019
3 Reads

Improved activity of α-L-arabinofuranosidase from Geobacillus vulcani GS90 by directed evolution: Investigation on thermal and alkaline stability.

Biotechnol Appl Biochem 2019 Jan 8;66(1):101-107. Epub 2018 Nov 8.

Department of Chemistry, İzmir Institute of Technology, İzmir, Turkey.

α-L-Arabinofuranosidase (Abf) is a potential enzyme because of its synergistic effect with other hemicellulases in agro-industrial field. In this study, directed evolution was applied to Abf from Geobacillus vulcani GS90 (GvAbf) using one round error-prone PCR and constructed a library of 73 enzyme variants of GvAbf. The activity screening of the enzyme variants was performed on soluble protein extracts using p-nitrophenyl α-L-arabinofuranoside as substrate. Read More

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http://dx.doi.org/10.1002/bab.1702DOI Listing
January 2019
1 Read

High recovery of intracellular recombinant HBsAg from Pichia pastoris via continuous pulsed laser cell disruption system optimized by response surface methodology.

Biotechnol Appl Biochem 2019 Jan 23;66(1):91-100. Epub 2018 Oct 23.

Production and Research Complex, Department of Recombinant Products, Pasteur Institute of Iran, Tehran, Iran.

In our previous study, we demonstrated that continuous power laser could be a clean, rapid, and convenient alternative to the other conventional disruption techniques for the release of recombinant hepatitis B surface antigen (rHBsAg) from Pichia pastoris. In the current work, we examined the effect of pulsed laser in the continuous laboratory-scale process on cell disruption. Design-of-experiments (DOE) methodology was used for optimization of cell disruption process to obtain the highest protein concentration in the disruption buffer. Read More

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http://dx.doi.org/10.1002/bab.1701DOI Listing
January 2019
2 Reads

Role and mechanism of ROS scavengers in alleviating NLRP3-mediated inflammation.

Biotechnol Appl Biochem 2019 Jan 25;66(1):4-13. Epub 2018 Oct 25.

Shanghai Key Laboratory for Veterinary and Biotechnology, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, People's Republic of China.

Inflammation, as a common immune response to various infections or injuries, can cause many dangerous and complicated diseases. Inflammasome is a protein complex playing a vital role in an inflammation process, and the nucleotide-binding oligomerization domain (NOD)-like receptor containing pyrin domain 3 (NLRP3) inflammasome has been the most-widely studied one. Recent evidence suggests the reactive oxygen species (ROS)-NLRP3 signaling pathway to be a possible NLRP3 inflammasome regulation model. Read More

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http://dx.doi.org/10.1002/bab.1700DOI Listing
January 2019
1 Read

Portable fluorescence-based microRNA detection system based on isothermal signal amplification technology.

Biotechnol Appl Biochem 2019 Jan 8;66(1):82-90. Epub 2018 Nov 8.

Anhui Province Key Laboratory of Medical Physics and Technology, Center of Medical Physics and Technology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, Anhui, People's Republic of China.

MicroRNAs (miRNAs) diagnostics can be useful for diagnosing or confirming miRNA abundance and are used in screening tests and to assess changes in miRNAs in vivo. At present, the use of traditional nucleic acid amplification assays to detect miRNAs has been limited in laboratory environment because of the time, equipment, and technical expertise required to perform these assays. A specialized, rapid affordable miRNA detection system is necessary when there are limited resources or point-of-care testing needs. Read More

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http://dx.doi.org/10.1002/bab.1699DOI Listing
January 2019
11 Reads

Cysteine synthase A overexpression in Corynebacterium glutamicum enhances l-isoleucine production.

Biotechnol Appl Biochem 2019 Jan 19;66(1):74-81. Epub 2018 Oct 19.

State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, People's Republic of China.

Cysteine synthase A (CysK) catalyzes the last reaction of l-cysteine synthesis in bacteria, but its moonlighting functions have been revealed recently. In this study, CysK was overexpressed in Corynebacterium glutamicum IWJ001, an l-isoleucine producer. Compared with the control IWJ001/pDXW-8, IWJ001/pDXW-8-cysK cells grew fast during log phase, and produced 26. Read More

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http://doi.wiley.com/10.1002/bab.1698
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http://dx.doi.org/10.1002/bab.1698DOI Listing
January 2019
16 Reads

Estrogen receptor-based multi-residue screening of bisphenol compounds in urine.

Biotechnol Appl Biochem 2019 Jan 29;66(1):68-73. Epub 2018 Oct 29.

Institute of Agricultural Resources and Environment, Jilin Academy of Agricultural Sciences, Changchun, People's Republic of China.

Human exposure to bisphenol compounds (BPs) has been implicated in the development of several chronic diseases. Instead of exploiting the traditional methods for determination of BPs, this work confirms that the human estrogen receptor α ligand binding domain (hERα-LBD) is a powerful recognition element that can be used to monitor multi-residue of BPs in urine samples by fluorescence polarization (FP) assay. Test parameters were optimized for the best performance. Read More

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http://dx.doi.org/10.1002/bab.1697DOI Listing
January 2019
2 Reads

Agrowastes utilization by Raoultella ornithinolytica for optimal extracellular peroxidase activity.

Biotechnol Appl Biochem 2019 Jan 5;66(1):60-67. Epub 2018 Nov 5.

SAMRC Microbial Water Quality Monitoring Centre, University of Fort Hare, Alice, South Africa.

The industrial applications and prospects of microbial peroxidase are on the upwards trend, thus necessitating the search for sources with high turnaround time. Actinobacterial species have been a major source of peroxidase for the obvious reasons of having robust metabolite expression capabilities. However, other bacteria species have been underexplored for peroxidase production, hence the motivation for the investigation into the peroxidase production potential of Raoultella ornithinolytica OKOH-1 (KX640917). Read More

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http://doi.wiley.com/10.1002/bab.1696
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http://dx.doi.org/10.1002/bab.1696DOI Listing
January 2019
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Enzyme-mediated transglycosylation of rutinose (6-O-α-l-rhamnosyl-d-glucose) to phenolic compounds by a diglycosidase from Acremonium sp. DSM 24697.

Biotechnol Appl Biochem 2019 Jan 23;66(1):53-59. Epub 2018 Oct 23.

INCITAP (CONICET-UNLPam) National Scientific and Technical Research Council, Department of Chemistry, Faculty of Natural Sciences, National University of La Pampa (UNLPam), Santa Rosa, La Pampa, Argentina.

The structure of the carbohydrate moiety of a natural phenolic glycoside can have a significant effect on the molecular interactions and physicochemical and pharmacokinetic properties of the entire compound, which may include anti-inflammatory and anticancer activities. The enzyme 6-O-α-rhamnosyl-β-glucosidase (EC 3.2. Read More

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http://dx.doi.org/10.1002/bab.1695DOI Listing
January 2019
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Novel bioactive porous starch-siloxane matrix for bone regeneration: Physicochemical, mechanical, and in vitro properties.

Biotechnol Appl Biochem 2019 Jan 10;66(1):43-52. Epub 2018 Oct 10.

Biomaterials Research Group, Department of Nanotechnology and Advanced Materials, Materials and Energy Research Center, Tehran, Iran.

So far, many studies have focused on biodegradable scaffolds for tissue engineering purposes. Herein, a starch-based biodegradable scaffold was fabricated by the freeze-drying method and cross-linked using a different concentration of 3-glycidoxypropyl-trimethoxysilane (GPTMS). Field emission scanning electron microscopy (FE-SEM) micrographs indicated an interconnected porous microstructure in which the porosity decreased as a function of starch and GPTMS content. Read More

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http://dx.doi.org/10.1002/bab.1694DOI Listing
January 2019
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Biochemical characteristics of a nitroreductase with diverse substrate specificity from Streptomyces mirabilis DUT001.

Biotechnol Appl Biochem 2019 Jan 2;66(1):33-42. Epub 2018 Oct 2.

State Key Laboratory of Fine Chemical Engineering and School of Life Science and Biotechnology, Dalian University of Technology, Dalian, People's Republic of China.

A nitroreductase-encoded gene from an efficient nitro-reducing bacterium Streptomyces mirabilis DUT001, named snr, was cloned and heterogeneously expressed in Escherichia coli. The purified Streptomyces nitroreductase SNR was a homodimer with an apparent subunit molecular weight of 24 kDa and preferred NADH to NADPH as a cofactor. By enzyme incubation and isothermal calorimetry experiments, flavin mononucleotide (FMN) was found to be the preferred flavin cofactor; the binding process was exothermic and primarily enthalpy driven. Read More

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http://dx.doi.org/10.1002/bab.1692DOI Listing
January 2019
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A novel nacre matrix protein hic24 in Hyriopsis cumingii is essential for calcium carbonate nucleation and involved in pearl formation.

Biotechnol Appl Biochem 2019 Jan 25;66(1):14-20. Epub 2018 Sep 25.

Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture, Shanghai, People's Republic of China.

Matrix proteins play important roles in molluscan shell biomineralization, which helps in the understanding of mechanisms associated with pearl formation. In this study, we characterized the gene encoding a novel shell-matrix protein, hic24, in Hyriopsis cumingii and investigated its structure and function. The full cDNA sequence of hic24 is 756 bp, with an open reading frame of 654 bp encoding 217 amino acids, including a signal peptide of 18 amino acids. Read More

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http://dx.doi.org/10.1002/bab.1690DOI Listing
January 2019
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miR-330-3p promotes lung cancer cells invasion, migration, and metastasis by directly targeting hSOD2b.

Biotechnol Appl Biochem 2019 Jan 15;66(1):21-32. Epub 2018 Oct 15.

Institute of Genomic Medicine, College of Pharmacy, Jinan University, Guangzhou, People's Republic of China.

Lung cancer is a serious threat to human health. Studies have revealed that human manganese superoxide dismutase (hSOD2) and miRNAs play an essential role in the metastasis process of lung cancer. However, the miRNAs that associated with hSOD2 and involved in metastasis, remain elusive. Read More

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http://dx.doi.org/10.1002/bab.1691DOI Listing
January 2019
9 Reads
1.362 Impact Factor

Epigenetic changes due to physical activity.

Biotechnol Appl Biochem 2018 Nov 19;65(6):761-767. Epub 2018 Sep 19.

Nutritional Health Research Center, Health and Nutrition Department, Lorestan University of Medical Sciences, Khorramabad, Iran.

One of the epigenetic-modifying factors is regular and continuous physical activity. This article attempts to investigate the effects of physical activity and exercise on changes in histone proteins and gene expression, as well as the effect of these exercises on the prevention of certain cancers and the ejection of age-related illnesses and cellular oxidation interactions. All of this is due to epigenetic changes and gene expression. Read More

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http://dx.doi.org/10.1002/bab.1689DOI Listing
November 2018
2 Reads
1.362 Impact Factor

Comparison of aqueous soluble proteins profile of Mycobacterium tuberculosis H37Rv and H37Ra and a Malaysian clinical isolate.

Biotechnol Appl Biochem 2018 Nov 31;65(6):876-882. Epub 2018 Aug 31.

School of Pharmaceutical Sciences, University Sains Malaysia, Minden, Penang, Malaysia.

Differentially expressed aqueous soluble proteins between Mycobacterium tuberculosis H37Ra and H37Rv were identified. The protein extracts were separated by two-dimensional gel electrophoresis followed by tandem mass spectrometric analysis. Twelve proteins were detected to be differentially expressed significantly between virulent strain H37Rv and attenuated strain H37Ra. Read More

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http://dx.doi.org/10.1002/bab.1687DOI Listing
November 2018
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Improving the thermal stability of azoreductase from Halomonas elongata by introducing a disulfide bond via site-directed mutagenesis.

Biotechnol Appl Biochem 2018 Nov 31;65(6):883-891. Epub 2018 Aug 31.

Extremophiles Laboratory, Department of Microbiology, School of Biology and Center of Excellence in Phylogeny of Living Organisms, College of Science, University of Tehran, Tehran, Iran.

Azoreductases mainly reduce azo dyes, the largest class of colorants, to colorless aromatic amines. AzoH, a new azoreductase from the halophilic bacterium, Halomonas elongata, has been recently cloned and expressed in Escherichia coli. The aim of this study was to improve thermal stability of this enzyme by introducing new disulfide bonds. Read More

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http://doi.wiley.com/10.1002/bab.1688
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http://dx.doi.org/10.1002/bab.1688DOI Listing
November 2018
16 Reads

Biosurfactants from probiotic bacteria: A review.

Biotechnol Appl Biochem 2018 Nov 19;65(6):768-783. Epub 2018 Sep 19.

National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran.

Among microorganisms, bacteria are the main group of biosurfactant-producing organisms. Different types of bacteria including Pseudomonas sp., Acinetobacter sp. Read More

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http://dx.doi.org/10.1002/bab.1686DOI Listing
November 2018
17 Reads