2,345 results match your criteria Biotechnology and Applied Biochemistry[Journal]


Silica Nanoparticle Assists Determining Liver Cancer Gene Sequence on Interdigitated Electrode Surface.

Biotechnol Appl Biochem 2020 Jul 6. Epub 2020 Jul 6.

Tianjin Third Central Hospital, School of Bioprocess Engineering, Arau, 02600, Malaysia.

A high-performance interdigitated electrode (IDE) biosensing surface was reported here by utilizing self-assembled silica nanoparticle (SiNP). The modified surface was used to evaluate the complementation of hairpin forming region from Mitoxantrone resistance gene 7 (MXR7; liver cancer related short gene). The conjugated SiNPs on 3-Aminopropyl Triethoxysilane functionalization were captured with probe sequence on IDE biosensing surface. Read More

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http://dx.doi.org/10.1002/bab.1980DOI Listing

Forty years of study on the thermostable β-glycosidase from S. solfataricus: Production, biochemical characterization and biotechnological applications.

Biotechnol Appl Biochem 2020 Jul 4. Epub 2020 Jul 4.

Research Institute on Terrestrial Ecosystems - National Research Council (CNR), Via P. Castellino 111 80131, Naples, Italy.

The aim of this paper is to make the point on the fortieth years study on the β-glycosidase from S. solfataricus. This enzyme represents one of the thermophilic biocatalysts the more extensively studied as witnessed by the numerous literature reports available since 1980. Read More

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http://dx.doi.org/10.1002/bab.1982DOI Listing

Immunodetection of urinary C-terminal telopeptide fragment of type II collagen: An osteoarthritis biomarker analysis.

Biotechnol Appl Biochem 2020 Jul 4. Epub 2020 Jul 4.

Department of Orthopedics, Shouguang Hospital of TCM, Weifang, Shandong Province, 262700, China.

The urinary C-terminal telopeptide fragment of type II collagen (uCTX-II) has been reported as the efficient blood-based biomarker for osteoarthritis, which affects knees, hands, spine, and hips. This study reports a sensing strategy with antibody-conjugated gold nanoparticles (GNP) on an interdigitated electrode (IDE) to determine uCTX-II. The GNP-antibody complex was chemically immobilized on the IDE surface through the amine linker. Read More

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http://dx.doi.org/10.1002/bab.1981DOI Listing

Downregulation of gap junctional intercellular communication and connexin 43 expression by bisphenol A in human granulosa cells.

Biotechnol Appl Biochem 2020 Jul 1. Epub 2020 Jul 1.

Department of Obstetrics and Gynecology, Kuo General Hospital, Tainan, Taiwan.

Gap junctional intercellular communication (GJIC) is the transfer of ions, metabolites, and second messengers between neighboring cells through intercellular junctions. Connexin 43 (Cx43) was found to be the type of gap junction protein responsible for human granulosa cells (GCs) and oocyte communication, which is required for folliculogenesis and oocyte maturation. Bisphenol A (BPA), an estrogenic-like endocrine-disrupting chemical, is one of the most widely produced chemicals around the world. Read More

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http://dx.doi.org/10.1002/bab.1979DOI Listing

Differential spectroscopic analysis of size-dependent phycobilisome from Spirulina maxima.

Biotechnol Appl Biochem 2020 Jun 29. Epub 2020 Jun 29.

Department of Biological Engineering, Inha University, Incheon, 402-751, Republic of Korea.

C-Phycocyanin (C-Pc), a photosynthetic pigment for use as a fluorescent indicator or in pharmaceutical, food, and cosmetic products, exists in a phycobilisome complex with allophycocyanin (APC), phycoerythrin (PE), and linker polypeptides. This heterogeneity makes it difficult to quantify phycobilisome composition in an ultraviolet-visible (UV-vis) spectrum. In this study, derivative analysis of UV-vis spectra was successfully applied to display the distinct wavelengths at which C-Pc, APC, and PE have maximal peaks. Read More

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http://dx.doi.org/10.1002/bab.1978DOI Listing

A novel approach to production of Chlorella protothecoides oil-loaded nanoparticles via electrospraying method: Modeling of critical parameters for particle sizing.

Biotechnol Appl Biochem 2020 Jun 27. Epub 2020 Jun 27.

Bioengineering Department, Faculty of Chemical and Metallurgical Engineering, Yıldız Technical University, Esenler, Istanbul, Turkey.

Bioactive compounds in algae have chain rings that protect the tissue from chemical damage and disease symptoms. In addition, algal bioactive agents have the ability to stimulate the immune system, protective and therapeutic effects against many diseases, including various types of cancers, coronary heart disease, premature aging, and arthritis. These bioactive compounds also have antioxidant, anticoagulant, antiviral, and anti-inflammatory properties. Read More

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http://dx.doi.org/10.1002/bab.1977DOI Listing

Light-driven catalysis with engineered enzymes and biomimetic systems.

Biotechnol Appl Biochem 2020 Jun 26. Epub 2020 Jun 26.

Department of Chemistry, University of Rochester, Rochester, NY, USA.

Efforts to drive catalytic reactions with light, inspired by natural processes like photosynthesis, have a long history and have seen significant recent growth. Successfully engineering systems using biomolecular and bioinspired catalysts to carry out light-driven chemical reactions capitalizes on advantages offered from the fields of biocatalysis and photocatalysis. In particular, driving reactions under mild conditions and in water, in which enzymes are operative, using sunlight as a renewable energy source yield environmentally friendly systems. Read More

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http://dx.doi.org/10.1002/bab.1976DOI Listing

Structural elucidation of selenocysteine insertion machinery of microalgal selenoprotein T and its transcriptional analysis.

Biotechnol Appl Biochem 2020 Jun 24. Epub 2020 Jun 24.

Microbial Processes and Technology Division, National Institute for Interdisciplinary Science and Technology (NIIST), Council of Scientific and Industrial Research (CSIR), Trivandrum, Kerala, India.

Essential trace element selenium in association with selenoproteins, which is found in almost all organisms except higher plants and fungi, is involved in various biological functions. Advancement in the field of whole genome sequencing and data analyzing bioinformatic tools led to the accumulation of genome information of organisms. However, selenoproteins are unique and it needs specialized genomics tool for its identification as well as characterization. Read More

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http://dx.doi.org/10.1002/bab.1974DOI Listing

Transient expression of the full-length glycoprotein from infectious hematopoietic necrosis virus in bean (Phaseolus vulgaris) leaves via agroinfiltration.

Biotechnol Appl Biochem 2020 Jun 24. Epub 2020 Jun 24.

Research Institute for Biotechnology and Bioengineering, Isfahan University of Technology, Isfahan, 84156-83111, Iran.

The glycoprotein of infectious hematopoietic necrosis virus (IHNV), the causative agent of acute disease in salmonids, is the only structural protein of the virus that can induce protective immunity in the fish host. Here, the reliability of bean (Phaseolus vulgaris) plant for the production of this viral protein was examined by the transient expression method. Using the syringe agroinfiltration method, leaves of bean plants were transformed with the expression construct encoding the full-length of IHNV glycoprotein (IHNV-G) gene. Read More

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http://dx.doi.org/10.1002/bab.1975DOI Listing

Rapid and label-free electrochemical DNA biosensor based on a facile one-step electrochemical synthesis of rGO-PPy-(L-Cys)-AuNPs nanocomposite for the HTLV-1 oligonucleotide detection.

Biotechnol Appl Biochem 2020 Jun 15. Epub 2020 Jun 15.

Inflammation and Inflammatory Disease Research Centre, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.

Human T cell leukemia virus type 1 (HTLV-1) as the first human retrovirus is currently a serious endemic health challenge. Despite the use of assorted molecular or serological assays for HTLV-1 detection, there are several limitations due to the lack of a confirmatory test that may affect the accuracy of the results. Herein, a novel label-free biosensor for the detection of HTLV-1 Tax gene has been reported. Read More

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http://dx.doi.org/10.1002/bab.1973DOI Listing

Organic solvent stability and long-term storage of myoglobin-based carbene transfer biocatalysts.

Biotechnol Appl Biochem 2020 Jun 15. Epub 2020 Jun 15.

Department of Chemistry, University of Rochester, Rochester, NY, USA.

Recent years have witnessed a rapid increase in the application of enzymes for chemical synthesis and manufacturing, including the industrial-scale synthesis of pharmaceuticals using multienzyme processes. From an operational standpoint, these bioprocesses often require robust biocatalysts capable of tolerating high concentrations of organic solvents and possessing long shelflife stability. In this work, we investigated the activity and stability of myoglobin (Mb)-based carbene transfer biocatalysts in the presence of organic solvents and after lyophilization. Read More

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http://dx.doi.org/10.1002/bab.1972DOI Listing

Conjugation of micro/nanocurcumin particles to ZnO nanoparticles changes the surface charge and hydrodynamic size thereby enhancing its antibacterial activity against Escherichia coli and Staphylococcus aureus.

Biotechnol Appl Biochem 2020 Jun 13. Epub 2020 Jun 13.

Department of Botany, Cotton University, Guwahati, India.

Nanobiotechnology-mediated synthesis of ZnO nanoparticles, micro/nanocurcumin, and curcumin-ZnO nanocomposites and their characterization followed by comparative study of their antibacterial, antioxidant, and iron-chelating efficiency at various dosages are discussed. Micro/nanocurcumin and ZnO nanoparticles were synthesized using curcumin and zinc nitrate as precursor and then conjugated by sonication to synthesize curcumin-ZnO nanocomposites. The synthesized nanoparticles were then characterized by using ultraviolet-visible spectroscopy, X-ray diffraction, Scanning electron microscopy, Fourier-transform infrared spectroscopy, and dynamic light scattering analysis. Read More

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http://dx.doi.org/10.1002/bab.1968DOI Listing
June 2020
1.362 Impact Factor

Determination of parvovirus antigen in the vaccine using time-resolved fluorescence immunoassay.

Biotechnol Appl Biochem 2020 Jun 13. Epub 2020 Jun 13.

Guangzhou Youdi Bio-technology Co., Ltd., Guangzhou, 510663, China.

As a highly contagious and potentially fatal disease of dogs, canine parvovirus type 2 (CPV-2) usually causes severe myocarditis and gastroenteritis, while vaccine injection has greatly reduced the incidence of CPV-2 diseases. However, there is currently a lack of simple and effective method for quantitative detection of CPV-2 in vaccine. Therefore, this study aims to prepare an accurate method to determine the CPV-2 antigen (CPV-2-Ag) in vaccine. Read More

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http://dx.doi.org/10.1002/bab.1967DOI Listing

Microfluidic-assisted fabrication of reverse micelle/PLGA hybrid microspheres for sustained vascular endothelial growth factor delivery.

Biotechnol Appl Biochem 2020 Jun 13. Epub 2020 Jun 13.

Marquette University School of Dentistry, Milwaukee, WI, USA.

In this study, poly (d, l-lactide-co-glycolide) (PLGA) composite microspheres containing anhydrous reverse micelle (R.M.) dipalmitoylphosphatidylcholine (DPPC) nanoparticles loaded vascular endothelial growth factor (VEGF) were produced using microfluidic platforms. Read More

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http://dx.doi.org/10.1002/bab.1971DOI Listing

Biological properties and structural characterization of a novel rhamnolipid like-biosurfactants produced by Lactobacillus casei subsp. casei TM1B.

Biotechnol Appl Biochem 2020 Jun 4. Epub 2020 Jun 4.

Department of Food Sciences and Nutrition, National School of Agro-Industrial Sciences, University of Ngaoundéré, Ngaoundéré, Cameroon.

Biosurfactants are microbial surface-active compounds with antimicrobial and antioxidant activities that display a range of physiological functions. In this study, a strain isolated from a Cameroonian fermented milk "pendidam" and identified as Lactobacillus casei subsp. casei TM1B was used for biosurfactants production. Read More

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http://dx.doi.org/10.1002/bab.1966DOI Listing

Metabolic engineering of Corynebacterium glutamicum WM001 to improve l-isoleucine production.

Biotechnol Appl Biochem 2020 May 31. Epub 2020 May 31.

State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, People's Republic of China.

In this study, l-isoleucine production in Corynebacterium glutamicum WM001 was improved by deleting three genes in the genome, replacing the native promoter of ilvA in the genome, and overexpression of five genes in an alr-based auxotrophic complementation expression system. The three genes deleted in the genome are alaT, brnQ, and alr. Deletion of alaT improved l-isoleucine production by increasing the supply of pyruvate, whereas deletion of brnQ improved l-isoleucine production by blocking the uptake of extracellular l-isoleucine. Read More

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http://dx.doi.org/10.1002/bab.1963DOI Listing

Development of novel strategies for higher fermentative biohydrogen recovery along with novel metabolites from organic wastes: The present state of the art.

Biotechnol Appl Biochem 2020 May 31. Epub 2020 May 31.

Department of Biotechnology, Dr. B. R Ambedkar National Institute of Technology, Jalandhar, 144 011, India.

Depletion of fossil fuels and environmental concern has compelled us to search for alternative fuel. Hydrogen is considered as a dream fuel as it has high energy content (142 kJ g ) and is not chemically bound to carbon. At present, fossil fuel-based methods for producing hydrogen require high-energy input, which makes the processes expensive. Read More

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http://dx.doi.org/10.1002/bab.1964DOI Listing

16S rRNA-functionalized multi-HCR concatemers in a signal amplification nanostructure for visual detection of Salmonella.

Biotechnol Appl Biochem 2020 May 30. Epub 2020 May 30.

Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun, China.

To prevent foodborne diseases and minimize their impacts, it is extremely important to develop a cost-effective and efficient bacterial detection assay for diagnostics, particularly in resource-poor settings. In this study, 16S rRNA from foodborne Salmonella was coupled with multiple HCR (hybridization chain reaction) concatemers and functionalized in a signal structure for lateral flow nucleic acid biosensor (LFNAB) detection. The 16S rRNA was incubated with two specific capture probes and multiple helper probes carrying the same initiator, to unwind its secondary structure and form an "initiators-on-a-string" complex. Read More

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http://dx.doi.org/10.1002/bab.1962DOI Listing

DNA-RNA complementation on silicon wafer for thyroid cancer determination.

Biotechnol Appl Biochem 2020 May 27. Epub 2020 May 27.

Department of Mammary and Thyroid Surgery, Central Hospital Affiliated to Shandong First Medical University, Jinan, 250013, China.

One of the current issues with thyroid tumor is early diagnosis as it makes the higher possibility of curing. This research was focused to detect and quantify the level of specific target sequence complementation of miR-222 with capture DNA sequence on interdigitated electrode (IDE) sensor. The aluminum electrode with the gap and finger sizes of 10 µm was fabricated on silicon wafer, further the surface was amine-functionalized for accommodating carboxylated-DNA probe. Read More

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http://dx.doi.org/10.1002/bab.1961DOI Listing

Purification of cell-derived Japanese encephalitis virus by dual-mode chromatography.

Biotechnol Appl Biochem 2020 May 27. Epub 2020 May 27.

College of Veterinary Medicine, Northwest A&F University, Yangling, People's Republic of China.

Purification of the enveloped virus poses a challenge as one must retain viral infectivity to preserve immunogenicity. The traditional process of virus purification is time-consuming, laborious and hard to scale up. Here, a rapid, simple and extensible laboratory program for the purification of Japanese encephalitis virus (JEV) was developed by using differential centrifugation, ultrafiltration, Sepharose 4 fast flow gel chromatography, and Capto Core 700 chromatography. Read More

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http://dx.doi.org/10.1002/bab.1960DOI Listing

Computational investigations on the dynamic binding effect of molecular tweezer CLR01 toward intrinsically disordered HIV-1 Nef.

Biotechnol Appl Biochem 2020 May 24. Epub 2020 May 24.

Bioinformatics Programming Laboratory, Department of Biotechnology, School of Bio-Sciences and Technology, Vellore Institute of Technology, Vellore, India.

Intrinsically disordered proteins (IDPs) are highly flexible molecules that undergo disorder to order transition through their interaction with other molecules. IDPs play a vital role in several biological processes ranging from molecular recognition to several human diseases through the protein-protein interaction. The dynamic flexibility of IDPs and their implications in several human diseases enable these molecules to serve as novel therapeutic targets. Read More

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http://dx.doi.org/10.1002/bab.1957DOI Listing

Application of lipase immobilized on a hydrophobic support for the synthesis of aromatic esters.

Biotechnol Appl Biochem 2020 May 21. Epub 2020 May 21.

Department of Exact Sciences and Technology, State University of Santa Cruz, Ilhéus, Brazil.

The present study aimed at preparing three biocatalysts via physical adsorption of lipases from Candida rugosa (CRL), Mucor javanicus, and Candida sp. on a hydrophobic and mesoporous support (Diaion HP-20). These biocatalysts were later applied to the synthesis of aromatic esters of apple peel and citrus (hexyl butyrate), apple and rose (geranyl butyrate), and apricot and pineapple (propyl butyrate). Read More

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http://dx.doi.org/10.1002/bab.1959DOI Listing

Effect of phenolic compounds from the leaves of Psidium guajava on the activity of three metabolism-related enzymes.

Biotechnol Appl Biochem 2020 May 20. Epub 2020 May 20.

Department of Pharmacognosy, Faculty of Pharmacy, Mansoura University, Mansoura, Egypt.

Enzyme activity modulation by synthetic compounds provide strategies combining the inhibitory and therapeutic mode of action of the confirmed inhibitors. However, natural modulators could offer a valuable alternative for synthetic ones for the treatment of different chronic diseases (diabetes, hypertension, cancer); due to the numerous side effects of the latter. In vitro screening assays were conducted for Psidium guajava leaf methanolic extract against three metabolism-related enzymes; α-amylase, tyrosinase, and hyaluronidase. Read More

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http://dx.doi.org/10.1002/bab.1956DOI Listing
May 2020
1.362 Impact Factor

Biosynthesis of bioactive tamarixetin in recombinant Escherichia coli.

Biotechnol Appl Biochem 2020 May 19. Epub 2020 May 19.

Department of Life Science and Biochemical Engineering, SunMoon University, Asan-si, Chungnam, Republic of Korea.

Tamarixetin, a monomethylated derivative of quercetin, has been reported to possess many important biological activities. In the present study, a whole cell biotransformation system was used for regiospecific methylation of quercetin to produce 4'-O-methylated quercetin (tamarixetin) using methyltransferase from Streptomyces sp. KCTC 0041BP in Escherichia coli Bl21 (DE3). Read More

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http://dx.doi.org/10.1002/bab.1958DOI Listing
May 2020
1.362 Impact Factor

Vanillic acid as phospholipase A and proteases inhibitor: In vitro and computational analyses.

Biotechnol Appl Biochem 2020 May 18. Epub 2020 May 18.

Biochemistry Laboratory, Department of Chemistry, Federal University of Lavras, Lavras, Brazil.

Enzymatic inhibition by natural compounds may represent a valuable adjuvant in snakebite serum therapy. The objective in this work was to evaluate possible in vitro interactions between vanillic acid and enzymes from Bothrops spp. and Crotalus durissus terrificus venoms, and also suggest a theory as how they interact based on molecular docking. Read More

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http://dx.doi.org/10.1002/bab.1943DOI Listing

Synthesis of substituted 2H-chromenes catalyzed by lipase immobilized on magnetic multiwalled carbon nanotubes.

Biotechnol Appl Biochem 2020 May 16. Epub 2020 May 16.

Key Laboratory of Molecular Enzymology and Engineering of Ministry of Education, School of Life Sciences, Jilin University, Changchun, People's Republic of China.

Porcine pancreas lipase (PPL) was immobilized on magnetic multiwalled carbon nanotubes successfully for the synthesis of substituted 2H-chromenes. The catalytic activity of immobilized PPL was much higher than that of free PPL. Effects of reaction medium, temperature, and enzyme dosage were also investigated. Read More

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http://dx.doi.org/10.1002/bab.1939DOI Listing

Screening and molecular characterization of new thermo- and ethanol-tolerant Acetobacter malorum strains isolated from two biomes Moroccan cactus fruits.

Biotechnol Appl Biochem 2020 May 15. Epub 2020 May 15.

Laboratory of Plant Biotechnology and Molecular Biology, Department of Biology, Faculty of Sciences, Moulay Ismail University, Zitoune, Meknes, Morocco.

Industrially, the sensitivity of acetic acid bacteria (AAB) to the high temperatures and the high ethanol concentrations is the major concerns for manufacturers. This study was conceived and designed to isolate and identify new thermo- and ethanol-tolerant AAB from Opuntia ficus-indica L. fruits. Read More

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http://dx.doi.org/10.1002/bab.1941DOI Listing

Development and optimization of N-acetylneuraminic acid biosensors in Bacillus subtilis.

Biotechnol Appl Biochem 2020 May 13. Epub 2020 May 13.

Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi, People's Republic of China.

Transcriptional factor (TF)-based metabolite-responsive biosensors are important tools for screening engineered enzymes with desired properties and for the dynamic regulation of biosynthetic pathways. However, TF-based biosensor construction is often constrained by undesired effects of TF-binding site sequence insertion on gene expression and unpredictable optimal TF expression levels. In the present study, a stepwise TF-based biosensor construction approach was developed using an N-acetylneuraminic acid (NeuAc) biosensor for Bacillus subtilis, as a case study. Read More

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http://dx.doi.org/10.1002/bab.1942DOI Listing

Harmful effects of high amounts of glucose on the immune system: An updated review.

Biotechnol Appl Biochem 2020 May 12. Epub 2020 May 12.

Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.

Release and storage of energy can be regulated by the metabolic parameter dependent on the central nervous system. Macrophages are one of the most professional antigen-presenting cells that are formed by the accumulation of dead or damaged cells or in response to the infection, which has the main function of phagocytosis, secretion of cytokines, and presenting antigen to T cells. A proper immune response is needed for the production of effector cytokines along with comprehensive and rapid cell proliferation and growth. Read More

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http://dx.doi.org/10.1002/bab.1938DOI Listing
May 2020
1.362 Impact Factor

RuBisCO nano enzyme for mimicking CO conversion system in plants.

Biotechnol Appl Biochem 2020 May 10. Epub 2020 May 10.

Department of Chemistry, Faculty of Sciences, Eskişehir Technical University, Yunus Emre Campus, Tepebaşı, Eskişehir, Turkey.

In this study, carbon dioxide (CO ) capture and conversion systems based on the combination of biomimetic systems with nano enzymes have been developed. The effectiveness of the developed system has been investigated toward CO conversion. For this aim, nano ribulose bisphosphate carboxylase/oxygenase (RuBisCO) enzyme that plays role in the Calvin cycle in photosynthesis has been synthesized in 93 nm size according to AmiNoAcid (monomer) Decorated and Light Underpinning Conjugation Approach (ANADOLUCA) method. Read More

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http://dx.doi.org/10.1002/bab.1937DOI Listing

Betaine-assisted recombinase polymerase assay for rapid hepatitis B virus detection.

Biotechnol Appl Biochem 2020 May 10. Epub 2020 May 10.

Department of Laboratory Medicine, North Sichuan Medical College, Nanchong, Sichuan, People's Republic of China.

Hepatitis B virus (HBV) is a worldwide epidemic pathogen that causes hepatitis B. On-site screening the HBV infection is of critical importance for preventing and diagnosing HBV infection. In this paper, a simple, visual, and rapid method for on-site detection of HBV-DNA has been developed. Read More

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http://dx.doi.org/10.1002/bab.1940DOI Listing

Protection against peripheral artery disease injury by Ruan Jian Qing Mai formula via metabolic programming.

Biotechnol Appl Biochem 2020 May 6. Epub 2020 May 6.

Shanghai TCM-Integrated Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, China.

Ruan Jian Qing Mai formula (RJQM), a multicomponent herbal formula, has been widely used to treat peripheral arterial disease (PAD) in China. However, its active compounds and mechanisms of action are still unknown. First, RNA sequencing analysis of 15 healthy and 16 PAD samples showed that 524 PAD differential genes were significantly enriched in Go Ontology (ribonucleotide metabolic process, oxidoreductase complex, and electron transfer activity), Kyoto Encyclopedia of Genes and Genomes (KEGG) and GSEA pathways (OXPHOS and TCA cycle), miRNA (MIR183), and kinase (PAK6). Read More

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http://dx.doi.org/10.1002/bab.1934DOI Listing

Correspondence: Importance of the validated serum biochemistry and hemogram parameters for rapid diagnosis and to prevent false negative results during COVID-19 pandemic.

Biotechnol Appl Biochem 2020 May 3. Epub 2020 May 3.

School of Medicine, Koç University, Istanbul, Turkey.

COVID-19 threatens millions of lives, especially elderly population and people with chronic diseases including diabetes, hypertension, cancer, and cardiovascular diseases. Rapid and effective diagnoses are vital for the isolation of infected people and starting treatment immediately to stop the spread of COVID-19 virus. Bioinformatics techniques such as artificial intelligence should be used for collecting the hemogram and serum biochemistry data of all COVID-19- infected people worldwide, even they do not show severe symptoms. Read More

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http://dx.doi.org/10.1002/bab.1936DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7267458PMC

Ghrelin ameliorates chronic obstructive pulmonary disease-associated infllammation and autophagy.

Biotechnol Appl Biochem 2020 May 1. Epub 2020 May 1.

Department of Respiratory and Critical Care Medicine, The Second Hospital of Hebei Medical University, Shijiazhuang, China.

Chronic obstructive pulmonary disease (COPD) is a chronic and devastating condition characterized by poor airflow and breath. Smoking and other environmental factors-caused inflammations triggered excessive autophagy of normal lung epithelial cells, eventually leading to impaired lung functions. Previous studies showed that ghrelin exhibited beneficial effects on patients with COPD. Read More

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http://dx.doi.org/10.1002/bab.1933DOI Listing

Cloning, characterization, and enzymatic identification of a new tryptophan decarboxylase from Ophiorrhiza pumila.

Biotechnol Appl Biochem 2020 Apr 30. Epub 2020 Apr 30.

Laboratory of Medicinal Plant Biotechnology, College of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou, People's Republic of China.

Tryptophan decarboxylase (TDC, EC 4.1.1. Read More

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http://dx.doi.org/10.1002/bab.1935DOI Listing

Overexpression of blue light receptor AaCRY1 improves artemisinin content in Artemisia annua L. .

Biotechnol Appl Biochem 2020 Apr 27. Epub 2020 Apr 27.

Key Laboratory of Urban Agriculture (South), Ministry of Agriculture, Plant Biotechnology Research Center, Fudan-SJTU-Nottingham Plant Biotechnology R&D Center, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, People's Republic of China.

Artemisinin, an effective antimalarial compound, is isolated from the medicinal plant Artemisia annua L. However, because of the low content of artemisinin in A. annua, the demand of artemisinin exceeds supply. Read More

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http://dx.doi.org/10.1002/bab.1931DOI Listing

Inoculation concentration modulating the secretion and accumulation pattern of exopolysaccharides in desert cyanobacterium Microcoleus vaginatus.

Biotechnol Appl Biochem 2020 Apr 26. Epub 2020 Apr 26.

School of Resources and Environmental Engineering, Wuhan University of Technology, Wuhan, People's Republic of China.

Cyanobacterial exopolysaccharides (EPS) accumulated during microalgal cultivation have significant application potential in antioxidation, pharmaceutical products, and so on. Inoculation concentration strongly affects the cultivation cost, biomass, and EPS accumulation. In this study, a high-EPS-excreted desert cyanobacterium Microcoleus vaginatus was isolated, and the effects of inoculation concentration on biomass, photosynthetic activity, and EPS accumulation were explored. Read More

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http://dx.doi.org/10.1002/bab.1930DOI Listing
April 2020
1.362 Impact Factor

Spectroscopic analysis of Muga silk nanoparticles synthesized by microwave method.

Biotechnol Appl Biochem 2020 Apr 26. Epub 2020 Apr 26.

Department of Physical Sciences, School of Basic and Applied Sciences, Central University of Punjab, Bathinda, Punjab, India.

Muga silk nanoparticles (MSNP) were synthesized using a microwave-assisted radiolysis method. The effect of microwave on the Muga protein secondary structures was analyzed. The evolution of the secondary structure from random coils to the β-sheets was determined by using FTIR, circular dichroism and X-ray diffraction techniques. Read More

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http://dx.doi.org/10.1002/bab.1932DOI Listing

Biomarkers of minimal residual disease in rituximab-treated patients with mixed cryoglobulinemia.

Biotechnol Appl Biochem 2020 Apr 25. Epub 2020 Apr 25.

Department of Translational and Precision Medicine, Sapienza University of Rome, Rome, Italy.

Hepatitis C virus (HCV) represents the major risk factor for mixed cryoglobulinemia (MC), a small-vessel vasculitis that may evolve into an overt B-cell non-Hodgkin's lymphoma. Here, we aimed to identify a biomarker signature for the early diagnosis of minimal residual disease (MRD). We assessed free light chains (FLCs), IgM k,and IgM λ heavy/light chain (HLC) pairs, and vascular endothelial growth factor (VEGF) in sera from 34 patients with MC vasculitis (32 HCV- and 2 HBV-related), treated with low-dose rituximab (RTX). Read More

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http://dx.doi.org/10.1002/bab.1929DOI Listing
April 2020
1.362 Impact Factor

Composite of medium-chain-length polyhydroxyalkanoates-co-methyl acrylate and carbon nanotubes as innovative electrodes modifier in microbial fuel cell.

Biotechnol Appl Biochem 2020 Apr 21. Epub 2020 Apr 21.

Department of Physics, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia.

A microbial fuel cell is a sustainable and environmental-friendly device that combines electricity generation and wastewater treatment through metabolic activities of microorganisms. However, low power output from inadequate electron transfer to the anode electrode hampers its practical implementation. Nanocomposites of oxidized carbon nanotubes and medium-chain-length polyhydroxyalkanoates (mcl-PHA) grafted with methyl acrylate monomers enhance the electrochemical function of electrodes in microbial fuel cell. Read More

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http://dx.doi.org/10.1002/bab.1928DOI Listing

The effect of IFN-β 1a on expression of MDA5 and RIG-1 in multiple sclerosis patients.

Biotechnol Appl Biochem 2020 Apr 20. Epub 2020 Apr 20.

Immunology of Infectious Diseases Research Center, Research Institute of Basic Medical Sciences, Rafsanjan University of Medical Sciences, Rafsanjan, Iran.

The aims of this study were to compare mRNA levels of melanoma differentiation-associated protein 5 (MDA5) and retinoic acid-inducible gene 1 (RIG-1) in multiple sclerosis (MS) patients in comparison to the healthy controls as well as investigating the effects of IFN-β 1a on the expression of these molecules. In this study, mRNA levels of MDA5 and RIG-1 in peripheral leukocytes of 30 new cases of MS patients and 35 healthy controls were evaluated using the real-time-PCR method. mRNA levels of MDA5 and RIG-1 were determined in the MS patients 6 months after treatment with standard doses of IFN-β 1a. Read More

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http://dx.doi.org/10.1002/bab.1920DOI Listing

Primary metabolites from overproducing microbial system using sustainable substrates.

Biotechnol Appl Biochem 2020 Apr 15. Epub 2020 Apr 15.

Department of Biotechnology, GIT, GITAM (Deemed to be University), Gandhi Nagar Campus, Rushikonda, Visakhapatnam, India.

Primary (or secondary) metabolites are produced by animals, plants, or microbial cell systems either intracellularly or extracellularly. Production capabilities of microbial cell systems for many types of primary metabolites have been exploited at a commercial scale. But the high production cost of metabolites is a big challenge for most of the bioprocess industries and commercial production needs to be achieved. Read More

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http://dx.doi.org/10.1002/bab.1927DOI Listing
April 2020
1.362 Impact Factor

Purification and properties of a laccase from the mushroom Agaricus sinodeliciosus.

Biotechnol Appl Biochem 2020 Apr 13. Epub 2020 Apr 13.

School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, People's Republic of China.

A homogeneous monomeric laccase (ASL) from Agaricus sinodeliciosus, with a molecular mass of 65 kDa, was isolated using ion-exchange chromatography (CM-cellulose and Q-Sepharose) and gel-filtration chromatography (Superdex 75). This laccase exhibited maximum activity at 50 °C and pH 5.0. Read More

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http://dx.doi.org/10.1002/bab.1926DOI Listing

Effect of nickel, cobalt, and iron on methanogenesis from methanol and cometabolic conversion of 1,2-dichloroethene by Methanosarcina barkeri.

Biotechnol Appl Biochem 2020 Apr 13. Epub 2020 Apr 13.

Laboratory of Microbiology, Wageningen University & Research, Wageningen, The Netherlands.

Methanogens are responsible for the last step in anaerobic digestion (AD), in which methane (a biofuel) is produced. Some methanogens can cometabolize chlorinated pollutants, contributing for their removal during AD. Methanogenic cofactors involved in cometabolic reductive dechlorination, such as F and cobalamin, contain metal ions (nickel, cobalt, iron) in their structure. Read More

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http://dx.doi.org/10.1002/bab.1925DOI Listing

Chemoselective N-H insertion catalyzed by a de novo carbene transferase.

Biotechnol Appl Biochem 2020 Apr 11. Epub 2020 Apr 11.

School of Biochemistry, University of Bristol, Bristol, UK.

The ability to perform organic reactions with chemoselectivity is of critical importance in synthesis. Recently, we reported that a de novo carbene transferase, a tetra-α-helical c-type heme-containing protein, C45, is proficient at N-H insertion reactions, proceeding via the intermolecular transfer of a metallocarbenoid intermediate into the N-H σ-bond to form a new N-C σ-bond. Here we demonstrate that C45 can also catalyse N-H insertion reactions chemoselectively, even when the substrate contains an unprotected hydroxyl group. Read More

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http://dx.doi.org/10.1002/bab.1924DOI Listing

Gene expression studies of Saccharum spontaneum, a wild relative of sugarcane in response to salinity stress.

Biotechnol Appl Biochem 2020 Apr 10. Epub 2020 Apr 10.

Genomics Laboratory, Division of Crop Improvement, ICAR-Sugarcane Breeding Institute, Coimbatore, India.

The study is aimed to assess the morphological, physiological, and molecular responses of seven Saccharum spontaneum clones for salinity stress. These clones (IND-07-1462, IND-07-1465, IND-07-1470, IND-07-1471, IND 16-1761, IND 16-1762, and IND 16-1763) were subjected to salinity stress at two different concentrations of electrical conductivity 6 and 8 ds/m after 60 days of planting. All seven genotypes showed a decrease in relative water content and nitrate reductase activity with an increase in severity of salt stress. Read More

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http://dx.doi.org/10.1002/bab.1923DOI Listing

Electroimmunodetection of cardiac C-reactive protein for determining myocardial Injury.

Biotechnol Appl Biochem 2020 Apr 10. Epub 2020 Apr 10.

Department of Cardiology, Hainan Cancer Hospital, Xiuying District, Haikou, Hainan, China.

C-reactive protein (CRP) is an acute phase reactant to be a marker of inflammation and has been correlated with the cardiac injury. An immunoassay was performed using anti-human CRP antibody on an InterDigitated electrode (IDE) sensor to determine and specify CRP concentration for diagnosing the condition of myocardial inflammation. To promote the detection, gold nanoparticle (GNP) was seeded on the aminated-IDE surface. Read More

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http://dx.doi.org/10.1002/bab.1921DOI Listing

Relationship between Sphk1/S1P and microRNAs in human cancers.

Biotechnol Appl Biochem 2020 Apr 10. Epub 2020 Apr 10.

Research Center for Molecular Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.

Sphingosine kinases type 1 (SphK1) is a key enzyme in the phosphorylation of sphingosine to sphingosine 1-phosphate (S1P). Different abnormalities in SphK1 functions may correspond with poor prognosis in various cancers. Additionally, upregulated SphK1/S1P could promote cancer cell proliferation, angiogenesis, mobility, invasion, and metastasis. Read More

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http://dx.doi.org/10.1002/bab.1922DOI Listing
April 2020
1.362 Impact Factor

Isolation and characterization of a novel nanobody for detection of GRP78 expressing cancer cells.

Biotechnol Appl Biochem 2020 Apr 9. Epub 2020 Apr 9.

Department of Biology, Faculty of Basic Sciences, Shahed University, Tehran, Iran.

Glucose-regulated protein 78 (GRP78) is an endoplasmic reticulum (ER) chaperone that has been shown that is overexpressed in cancer cells. Overexpression of GRP78 on cancer cells makes this molecule a suitable candidate for cancer detection and targeted therapy. VHH is the binding fragment of camelid heavy-chain antibodies also known as "nanobody. Read More

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http://dx.doi.org/10.1002/bab.1916DOI Listing
April 2020
1.362 Impact Factor

Di-2-picolylamine triggers caspase-independent apoptosis by inducing oxidative stress in human liver hepatocellular carcinoma cells.

Biotechnol Appl Biochem 2020 Apr 6. Epub 2020 Apr 6.

Discipline of Medical Biochemistry, School of Laboratory Medicine and Medical Science, University of KwaZulu-Natal, Durban, South Africa.

Di-2-picolylamine (DPA) is an organic compound that has been shown to possess antioxidant properties when conjugated to form a metal complex. The basis of this study was to determine the effects of DPA on the proliferation and apoptosis of human hepatocellular carcinoma cells and elucidate the possible mechanisms. The methylthiazol tetrazolium assay served to measure cell viability and generated an IC of 1591 µM. Read More

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http://dx.doi.org/10.1002/bab.1918DOI Listing
April 2020
1.362 Impact Factor