360,244 results match your criteria Biotechnology and Applied Biochemistry[Journal]


Porcine-Derived Biomaterials in Tissue Engineering and Reconstructive Surgery: Considerations and Alternatives in Muslim Patients.

J Tissue Eng Regen Med 2018 Dec 16. Epub 2018 Dec 16.

Department of Plastic Surgery, Hand Surgery and Burn Centre, Faculty of Medicine, RWTH University Hospital, Aachen, Germany.

During the last three decades, tissue engineering and reconstructive surgery have become standard therapeutic options in the world of medicine. Several biomaterials, either alone, or in combination with cultured cellular products, have been introduced to compensate for the scarcity of autologous donor tissue or to improve healing in a variety of surgical specialties e.g. Read More

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December 2018

Orf virus infection of human keratinocytes and dermal fibroblasts: limited virus detection and interference with intercellular adhesion molecule-1 up-regulation.

Exp Dermatol 2018 Dec 15. Epub 2018 Dec 15.

Institute of Immunology/Molecular Pathogenesis, Center for Biotechnology and Biomedicine, College of Veterinary Medicine, Leipzig University, Leipzig, Germany.

Orf virus (Parapoxvirus ovis, ORFV) is a dermatotropic virus causing pustular dermatitis in small ruminants and humans. We analyzed isolated human primary keratinocytes (KC) and dermal fibroblasts (FB) for cell death and virus replication by infection with a patient-derived ORFV isolate. ORFV infection was associated with rapid induction of cell death in KC allowing for considerable virus removal. Read More

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December 2018

SOG1-dependent NAC103 modulates the DNA damage response as a transcriptional regulator in Arabidopsis.

Plant J 2018 Dec 15. Epub 2018 Dec 15.

Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, 29 Geumgu-gil, Jeongeup-si, Jeollabuk-do, 56212, Republic of Korea.

The plant-specific transcription factor (TF) NAC103 was previously reported to modulate the unfolded protein response in Arabidopsis under endoplasmic reticulum (ER) stress. Alternatively, we here report that the NAC103 is involved in the downstream signaling of SOG1, a master regulator for expression of DNA damage response (DDR) genes induced by genotoxic stress. Arabidopsis NAC103 expression was strongly induced by genotoxic stress and nac103 mutants displayed substantial inhibition of DDR gene expression after gamma radiation or radiomimetic zeocin treatment. Read More

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December 2018

Genome analysis and -omics approaches provide new insights into the biodegradation potential of Rhodococcus.

Appl Microbiol Biotechnol 2018 Dec 15. Epub 2018 Dec 15.

Department of Biotechnology and Biosciences, University of Milano-Bicocca, Piazza della Scienza 2, 20126, Milan, Italy.

The past few years observed a breakthrough of genome sequences of bacteria of Rhodococcus genus with significant biodegradation abilities. Invaluable knowledge from genome data and their functional analysis can be applied to develop and design strategies for attenuating damages caused by hydrocarbon contamination. With the advent of high-throughput -omic technologies, it is currently possible to utilize the functional properties of diverse catabolic genes, analyze an entire system at the level of molecule (DNA, RNA, protein, and metabolite), simultaneously predict and construct catabolic degradation pathways. Read More

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December 2018

Influence of seed priming with iron and/or zinc in the nucleolar activity and protein content of bread wheat.

Protoplasma 2018 Dec 15. Epub 2018 Dec 15.

Biosystems and Integrative Sciences Institute (BioISI), University of Tras-os-Montes and Alto Douro (UTAD), 5000-801, Vila Real, Portugal.

Seed priming with iron (Fe) and/or zinc (Zn) can overcome the reduced availability of these micronutrients in soils and crops, but suitable dosages should be predetermined. Nucleolus responds to stress, such as cytotoxicity, with alterations perceivable by cytogenetic analyses. This work intends to study how seed priming with Fe and/or Zn affects the nucleolar activity in roots and the total soluble protein content in the flour of bread wheat cv. Read More

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December 2018

Resveratrol Protects Against Renal Damage via Attenuation of Inflammation and Oxidative Stress in High-Fat-Diet-Induced Obese Mice.

Inflammation 2018 Dec 15. Epub 2018 Dec 15.

College of Animal Science and Technology, Nanjing Agricultural University, No. 1 Weigang, Nanjing, 210095, Jiangsu, People's Republic of China.

Oxidative stress and inflammation play an important role in the chronic kidney disease associated with obesity. Resveratrol (RSV) has been reported to exhibit a wide range of biological activities including antioxidant and anti-inflammatory properties. The objective of the present study was to investigate the effects of RSV on renal inflammation and oxidative stress in obese mice induced by high-fat diet. Read More

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December 2018

Novel flourescent spiroborate esters: potential therapeutic agents in in vitro cancer models.

Mol Biol Rep 2018 Dec 15. Epub 2018 Dec 15.

Department of Biochemistry, University of Kerala, Thiruvananthapuram, Kerala, India.

The current treatment system in cancer therapy, which includes chemotherapy/radiotherapy is expensive and often deleterious to surrounding healthy tissue. Presently, several medicinal plants and their constituents are in use to manage the development and progression of these diseases.They have been found effective, safe, and less expensive. Read More

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December 2018

Bacterial exopolysaccharide promotes acid tolerance in Bacillus amyloliquefaciens and improves soil aggregation.

Mol Biol Rep 2018 Dec 15. Epub 2018 Dec 15.

Department of Agricultural Biotechnology, Assam Agricultural University, Jorhat, Assam, India.

In this paper we report the isolation and taxonomic characterization of exopolysaccharide (EPS) producing bacteria followed by the role of EPS in conferring acid tolerance to the soil bacteria Bacillus amyloliquefaciens p16. The role of EPS in promoting soil aggregation is also presented. A total of 75 isolates were tested for acid tolerance and biofilm production under acid stress of which, 54 isolates were further tested for EPS production. Read More

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December 2018

Therapeutic targeting of angiogenesis molecular pathways in angiogenesis-dependent diseases.

Biomed Pharmacother 2018 Dec 13;110:775-785. Epub 2018 Dec 13.

Biotechnology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran; Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran; Molecular Medicine Research Centre, Tabriz University of Medical Sciences, Tabriz, Iran. Electronic address:

Angiogenesis is a critical step in the progression of almost all human malignancies and some other life-threatening diseases. Anti-angiogenic therapy is a novel and effective approach for treatment of angiogenesis-dependent diseases such as cancer, diabetic retinopathy, and age-related macular degeneration. In this article, we will review the main strategies developed for anti-angiogenic therapies beside their clinical applications, the major challenges, and the latest advances in the development of anti-angiogenesis-based targeted therapies. Read More

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December 2018

A general strategy to fabricate soft magnetic CuFeO@SiO nanofibrous membranes as efficient and recyclable Fenton-like catalysts.

J Colloid Interface Sci 2018 Dec 8;538:620-629. Epub 2018 Dec 8.

State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, China; Key Laboratory of Textile Science and Technology, Ministry of Education, College of Textiles, Donghua University, Shanghai 201620, China; Innovation Center for Textile Science and Technology, Donghua University, Shanghai 200051, China. Electronic address:

Fenton or Fenton-like technique, as one of the advanced oxidation processes, plays a significant role in the removal of non-easily degradable organic pollutants; however, most of such catalysts are fragile with poor structural integrity under large deformation, thereby restricting their wide applications. Herein, soft copper ferrite nanostructures functionalized silica nanofibrous membranes (CuFeO@SNM) were fabricated through a novel strategy with the combination of in-situ dopamine polymerization, ion adsorption, and cohesive precipitation method. Benefiting from the high metallic ion adsorption capacity of polydopamine together with the rapid co-precipitation of adsorbed ions on fiber surface in alkaline solution, the membranes possessed homogenously distributed nanostructured CuFeO, large specific surface area, and high pore volume, which are a benefit for the improvement of Fenton-like catalytic activity towards organic pollutants decomposition. Read More

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December 2018

White spot syndrome virus infection activates Caspase 1-mediated cell death in crustacean.

Virology 2018 Dec 13;528:37-47. Epub 2018 Dec 13.

College of Life Sciences and Laboratory for Marine Biology and Biotechnology of Qingdao National Laboratory for Marine Science and Technology, Zhejiang University, Hangzhou 310058, People's Republic of China. Electronic address:

In vertebrates, pyroptosis is an intense inflammatory form of programmed cell death. This death pathway is critical for controlling pathogenic infection. In invertebrates, however, due to the lack of adaptive immune response, it is still elusive whether Caspase 1-dependent cell death pathway exists. Read More

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December 2018

Customized protein glycosylation to improve biopharmaceutical function and targeting.

Curr Opin Biotechnol 2018 Dec 13;60:17-28. Epub 2018 Dec 13.

VIB-UGent Center for Medical Biotechnology, Belgium; Department of Biochemistry and Microbiology, Ghent University, Technologiepark 927, B-9052 Gent, Belgium. Electronic address:

For a long time, glycoprotein production has been limited by the inherent properties of production hosts. Glycosylation of biopharmaceuticals has been regarded as a necessary evil, often needed for protein folding or function, but also a source of heterogeneity, complicating downstream processing and product characterization. This has strongly determined the choice of production hosts. Read More

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December 2018

DNA variants in Helicobacter pylori infected patients with chronic gastritis, dysplasia and gastric cancer.

Adv Med Sci 2018 Dec 13;64(1):79-84. Epub 2018 Dec 13.

Department of Biochemistry and Biotechnology, Poznan University of Life Sciences, Poznan, Poland; Institute of Human Genetics, Polish Academy of Sciences, Poznan, Poland. Electronic address:

Purpose: The main scope of this study was to evaluate the importance of selected DNA variants for developing inflammation of gastric mucosa and carcinogenesis in gastrointestinal diseases in patients infected with Helicobacter pylori.

Patients And Methods: Patients subjected to analysis constituted a group of 131 consecutive cases, with control groups consisting of 100 healthy volunteers and 13 dyspeptic patients. Molecular analysis included the following genes: TP53 (c. Read More

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December 2018

Comparison of F FDG PET-CT AND CECT in pretreatment staging of adults with Hodgkin's lymphoma.

Leuk Res 2018 Dec 5;76:48-52. Epub 2018 Dec 5.

Hematology, Department of Translation and Precision Medicine, "Sapienza" University of Rome, Italy; Hematology Unit, S. Maria Goretti Hospital, AUSL Latina, Italy. Electronic address:

We compared 2-[fluorine-18] fluoro-2-deoxy-d-glucose PET-CT and contrast-enhanced computed tomography (CECT) in 62 consecutive patients with newly diagnosed Hodgkin Lymphoma (HL), aiming to provide evidences that may spare CECT from the staging procedures of HL patients. Among a total of 1448 nodal sites examined, disease involvement was detected in 232 (16%) and 280 (19.3%) nodal areas by CECT and PET-CT, respectively (P < 0. Read More

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December 2018

The whole blood transcriptome at the time of maternal recognition of pregnancy in pigs reflects certain alterations in gene expression within the endometrium and the myometrium.

Theriogenology 2018 Nov 22;126:159-165. Epub 2018 Nov 22.

Department of Anatomy and Animal Physiology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego 1A, 10-718, Olsztyn, Poland.

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November 2018

Response of the biomining Acidithiobacillus ferrooxidans to high cadmium concentrations.

J Proteomics 2018 Dec 13. Epub 2018 Dec 13.

Laboratory of Molecular Microbiology and Biotechnology, Department of Biology, Faculty of Sciences, University of Chile, Santiago, Chile. Electronic address:

Cadmium is a heavy metal present in contaminated soils. It has no biological role but when entering cells generates DNA damage, overexpression of stress response proteins and misfolded proteins, amongst other deleterious effects. Acidithiobacillus ferrooxidans is an acidophilic bacterium resisting high concentrations of heavy metals such as cadmium. Read More

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December 2018

Engineering of the citrate exporter protein enables high citric acid production in Aspergillus niger.

Metab Eng 2018 Dec 13. Epub 2018 Dec 13.

Austrian Centre of Industrial Biotechnology (ACIB GmbH), Muthgasse 18, Vienna, Austria; Department of Biotechnology, University of Natural Resources and Life Sciences Vienna, Muthgasse 18, Vienna, Austria; CD Laboratory for Biotechnology of Glycerol, Muthgasse 18, Vienna, Austria. Electronic address:

We engineered Aspergillus niger using a gene responsible for citric acid transport, which has a significant impact on citric acid secretion when overexpressed.. The transport gene was identified by a homology search using an itaconic acid transporter from Ustilago maydis as template. Read More

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December 2018

Production of biofuels and chemicals from xylose using native and engineered yeast strains.

Biotechnol Adv 2018 Dec 13. Epub 2018 Dec 13.

Department of Agricultural Biotechnology and Center for Food and Bioconvergence, Seoul National University, Seoul 08826, South Korea. Electronic address:

Numerous metabolic engineering strategies have allowed yeasts to efficiently assimilate xylose, the second most abundant sugar component of lignocellulosic biomass. During the investigation of xylose utilization by yeasts, a global rewiring of metabolic networks upon xylose cultivation has been captured, as opposed to a pattern of glucose repression. A clear understanding of the xylose-induced metabolic reprogramming in yeast would shed light on the optimization of yeast-based bioprocesses to produce biofuels and chemicals using xylose. Read More

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December 2018

Trichoderma harzianum mitigates salt stress in cucumber via multiple responses.

Ecotoxicol Environ Saf 2018 Dec 13;170:436-445. Epub 2018 Dec 13.

School of Life Science and Agronomy, Zhoukou Normal University, Zhoukou, Henan 466001, PR China.

Trichoderma harzianum T-soybean plays an important role in controlling soybean root rot disease. However, the mechanism by which it improves plant tolerance to salt stress is not clear. In this study, we investigated the possible mechanism of T-soybean in mitigating the damage caused by salt stress in Cucumis sativus L plants. Read More

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December 2018

Hematopoietic tissue of Macrobrachium rosenbergii plays dual roles as a source of hemocyte hematopoiesis and as a defensive mechanism against Macrobrachium rosenbergii nodavirus infection.

Fish Shellfish Immunol 2018 Dec 13. Epub 2018 Dec 13.

Center of Excellence for Shrimp Molecular Biology and Biotechnology (Centex Shrimp), Faculty of Science, Mahidol University, Rama VI Road, Bangkok, 10400, Thailand; Nakhonsawan Campus, Mahidol University, Nakhonsawan, 60130, Thailand. Electronic address:

White tail disease caused by Macrobrachium rosenbergii nodavirus (MrNV) infection takes place only in nauplii, not adults, of M. rosenbergii prawn. Hemocyte homeostasis and immune-related functions derived from the hematopoietic tissue (Hpt) in adult prawn are presumed to play roles in resisting viral infection. Read More

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December 2018

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Genomics Proteomics Bioinformatics 2018 Dec 13. Epub 2018 Dec 13.

State Key Laboratory of Protein and Plant Gene Research, Peking-Tsinghua Center for Life Sciences, School of Advanced Agriculture Sciences and School of Life Sciences, Peking University, Beijing 100871, China. Electronic address:

Rice is a major cereal crop for China. The development of the "three-line" hybrid rice system based on cytoplasmic male sterility in the 1970s (first-generation) and the "two-line" hybrid rice system based on photoperiod- and thermo-sensitive genic male-sterile lines (second-generation) in the 1980s has contributed significantly to rice yield increase and food security in China. Here we describe the development and implementation of the "third-generation" hybrid rice breeding system that is based on a transgenic approach to propagate and utilize stable recessive nuclear male sterile lines, and as such, the male sterile line and hybrid rice produced using such a system is non-transgenic. Read More

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December 2018

Structural and functional characterization of a family GH53 β-1,4-galactanase from Bacteroides thetaiotaomicron that facilitates degradation of prebiotic galactooligosaccharides.

J Struct Biol 2018 Dec 13. Epub 2018 Dec 13.

Microbial Physiology, Groningen Biomolecular Sciences and Biotechnology Institute (GBB), University of Groningen, Nijenborgh 7, 9747AG Groningen, The Netherlands. Electronic address:

Galactooligosaccharides (GOS) are prebiotic compounds synthesized from lactose using bacterial enzymes and are known to stimulate growth of beneficial bifidobacteria in the human colon. Bacteroides thetaiotaomicron is a prominent human colon commensal bacterial species that hydrolyzes GOS using an extracellular Glycosyl Hydrolase (GH) family GH53 endo-galactanase enzyme (BTGH53), releasing galactose-based products for growth. Here we dissect the molecular basis for GOS activity of this B. Read More

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December 2018

A comprehensive analysis of the protein-ligand interactions in crystal structures of Mycobacterium tuberculosis EthR.

Biochim Biophys Acta Proteins Proteom 2018 Dec 13. Epub 2018 Dec 13.

Unité Microbiologie, Bioorganique et Macromoléculaire (CP206/04), département R3D, Faculté de Pharmacie, Université Libre de Bruxelles, B-1050 Brussels, Belgium. Electronic address:

The Mycobacterium tuberculosis EthR is a member of the TetR family of repressors, controlling the expression of EthA, a mono-oxygenase responsible for the bioactivation of the prodrug ethionamide. This protein was established as a promising therapeutic target against tuberculosis, allowing, when inhibited by a drug-like molecule, to boost the action of ethionamide. Dozens of EthR crystal structures have been solved in complex with ligands. Read More

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December 2018

Mediatory role of BLT2 in the proliferation of KRAS mutant colorectal cancer cells.

Biochim Biophys Acta Mol Cell Res 2018 Dec 13. Epub 2018 Dec 13.

School of Life Sciences and Biotechnology, Korea University, Seoul 136-701, Republic of Korea. Electronic address:

Inflammatory lipid mediators play various roles in colorectal cancer progression through complex pathways. However, the mechanism by which lipoxygenase-derived inflammatory lipid mediators contribute to colorectal cancer progression remains elusive. In this study, we found that BLT2, a cell surface GPCR for leukotriene B and 12‑hydroxyeicosatetraenoic acid, is highly upregulated in KRAS mutant LOVO and SW480 colorectal cancer cells and plays critical roles in mediating proliferation through activation of phosphatidylinositol 3‑kinase (PI3K)/protein kinase B (Akt) and subsequent upregulation of cyclin D1. Read More

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December 2018

Corrigendum to "Comparative analysis of novel autoantibody isotypes against citrullinatedinter-alpha-trypsin inhibitor heavy chain 3 (ITIH3) 542-556 peptide in serum from Taiwanese females with rheumatoid arthritis, primary Sjögren's syndrome and secondary Sjögren's syndrome in rheumatoid arthritis" [J Proteomics 141: (2016) 1-11].

J Proteomics 2018 Dec 12. Epub 2018 Dec 12.

School of Medical Laboratory Science and Biotechnology, College of Medical Science and Technology, Taipei Medical University, Taipei 11031, Taiwan; PhD Program for Cancer Biology and Drug Discovery, College of Medical Science and Technology, Taipei Medical University and Academia Sinica, Taipei 11031, Taiwan. Electronic address:

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December 2018

Corrigendum to "Isotypes of autoantibodies against differentially expressed novel malondialdehyde-modifiedpeptide adducts in serum of Taiwanese women with rheumatoid arthritis" [J Proteomics 170: (2018) 141-150].

J Proteomics 2018 Dec 12. Epub 2018 Dec 12.

Department of Biotechnology and Animal Science, National Ilan University, Ilan 26047, Taiwan; School of Medical Laboratory Science and Biotechnology, College of Medical Science and Technology, Taipei Medical University, Taipei 11031, Taiwan. Electronic address:

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December 2018

Increasing complexity and interactions of oxidative stress in chronic respiratory diseases: An emerging need for novel drug delivery systems.

Chem Biol Interact 2018 Dec 13. Epub 2018 Dec 13.

Priority Research Centre for Healthy Lungs, Hunter Medical Research Institute (HMRI) & School of Biomedical Sciences and Pharmacy, University of Newcastle, Callaghan, NSW, 2308, Australia; Faculty of Science, University of Technology Sydney, Ultimo, NSW, 2007, Australia; Centre for Inflammation, Centenary Institute, Sydney, NSW, 2050, Australia.

Oxidative stress is intensely involved in enhancing the severity of various chronic respiratory diseases (CRDs) including asthma, chronic obstructive pulmonary disease (COPD), infections and lung cancer. Even though there are various existing anti-inflammatory therapies, which are not enough to control the inflammation caused due to various contributing factors such as anti-inflammatory genes and antioxidant enzymes. This leads to an urgent need of novel drug delivery systems to combat the oxidative stress. Read More

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December 2018

Pyrrolidinone-bearing methylated and halogenated benzenesulfonamides as inhibitors of carbonic anhydrases.

Bioorg Med Chem 2018 Dec 6. Epub 2018 Dec 6.

Department of Biothermodynamics and Drug Design, Institute of Biotechnology, Life Sciences Center, Vilnius University, Saulėtekio 7, Vilnius LT-10257, Lithuania.

Two series of benzenesulfonamides bearing methyl groups at ortho/ortho or meta/ortho positions and a pyrrolidinone moiety at para position were synthesized and tested as inhibitors of the twelve catalytically active human carbonic anhydrase (CA) isoforms. Observed binding affinities were determined by fluorescent thermal shift assay and intrinsic binding affinities representing the binding of benzenesulfonamide anion to the Zn(II)-bound water form of CA were calculated. Introduction of dimethyl groups into benzenesulfonamide ring decreased the binding affinity to almost all CA isoforms, but gained in selectivity towards one CA isoform. Read More

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December 2018

Interlaboratory comparison about feasibility of insoluble particulate matter test for injections with reduced test volume in light obscuration method.

Biologicals 2018 Dec 12. Epub 2018 Dec 12.

Division of Biological Chemistry and Biologicals, National Institute of Health Sciences, Tonomachi 3-25-26, Kawasaki-ku, Kanagawa, 210-9501, Japan.

Insoluble particulate matter test for injections in pharmacopoeia is mandatory for parenteral drug products. In this test using light obscuration, four measurements of at least 5-mL are required. Since therapeutic protein injections of low dosage volumes are getting more popular, reduction of test volumes is desired. Read More

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December 2018

Mutation screening of SLC52A3, C19orf12, and TARDBP in Iranian ALS patients.

Neurobiol Aging 2018 Nov 16. Epub 2018 Nov 16.

School of Biology, College of Science, University of Tehran, Tehran, Iran; Department of Biotechnology, College of Science, University of Tehran, Tehran, Iran. Electronic address:

Mutations in the same gene are sometimes the cause of different clinically diagnosed neurologic disorders; this emphasizes interrelationships between various neurologic diseases. In this light, we screened SLC52A3, which is the cause of Brown-Vialetto-Van Laere syndrome, and C19orf12, which is the cause of neurodegeneration with brain iron accumulation in 60 Iranian amyotrophic lateral sclerosis (ALS) patients without mutations in the 2 most important ALS-causing genes, SOD1 and C9orf72. To the best of our knowledge, neither SLC52A3 nor C19orf12 has been mutation-screened previously in ALS cohorts. Read More

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November 2018

Loss of ATM positively regulates Rac1 activity and cellular migration through oxidative stress.

Biochem Biophys Res Commun 2018 Dec 13. Epub 2018 Dec 13.

Department of Chemistry, High Point University, High Point, NC, 27268, USA. Electronic address:

Ataxia-telangiectasia mutated (ATM) is a serine-threonine kinase that is integral in the response to DNA double-stranded breaks (DSBs). Cells and tissues lacking ATM are prone to tumor development and enhanced tumor cell migration and invasion. Interestingly, ATM-deficient cells exhibit high levels of oxidative stress; however, the direct mechanism whereby ATM-associated oxidative stress may contribute to the cancer phenotype remains largely unexplored. Read More

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December 2018

Microwave assisted κ-carrageenan capped silver nanocomposites for eradication of bacterial biofilms.

Carbohydr Polym 2019 Feb 14;206:854-862. Epub 2018 Nov 14.

Centre for Nanotechnology, Indian Institute of Technology Roorkee, Roorkee, 247667, Uttarakhand, India; Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, 247667, Uttarakhand, India. Electronic address:

Maturation of bacterial biofilms and their resistance to recurrent antimicrobial agents results in convoluted infectious diseases. In the current study, kappa-Carrageenan (κ-Carrageenan/CRG), was used to formulate CRG-silver nanocomposites through a facile microwave green synthesis approach. CRG-Ag nanoparticles of size 50 ± 10 nm were obtained by using CRG as a reducing and stabilizing agent. Read More

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February 2019

Bio-based polymer colorants from nonaqueous reactive dyeing of regenerated cellulose for plastics and textiles.

Carbohydr Polym 2019 Feb 19;206:734-741. Epub 2018 Nov 19.

Key Lab of Science and Technology of Eco-textile, Ministry of Education, College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, People's Republic of China; Key Lab of High Performance Fibers & Products, Ministry of Education, Donghua University, Shanghai 201620, People's Republic of China. Electronic address:

A general way to prepare bio-based polymer colorant by reactive dyeing of regenerated cellulose (RC) in dimethyl sulfoxide (DMSO) was established. Up to 40% higher dye utilization was achieved compared with aqueous reactive dyeing of RC for two reasons. 1. Read More

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February 2019

Development of modified montmorillonite-bacterial cellulose nanocomposites as a novel substitute for burn skin and tissue regeneration.

Carbohydr Polym 2019 Feb 13;206:548-556. Epub 2018 Nov 13.

Biotechnology Program, Department of Environmental Sciences, COMSATS University Islamabad, Abbottabad Campus 22060, Pakistan. Electronic address:

Bacterial cellulose (BC) is a promising biopolymer with wound healing and tissue regenerative properties but lack of antimicrobial property limits its biomedical applications. Therefore, current study was proposed to combine wound healing property of BC with antimicrobial activity of montmorillonite (MMT) and modified montmorillonites (Cu-MMT, Na-MMT and Ca-MMT) to design novel artificial substitute for burns. Designed nanocomposites were characterized through Fe-SEM, FTIR and XRD. Read More

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February 2019

Inhibition of bacterial adhesion and biofilm formation of sulfonated chitosan against Pseudomonas aeruginosa.

Carbohydr Polym 2019 Feb 10;206:412-419. Epub 2018 Nov 10.

Jiangsu Key Laboratory of Regional Resource Exploitation and Medicinal Research, Huaiyin Institute of Technology, Huaian 223003, PR China.

The exploitation of chitosan derivatives as safe and natural polymers against microorganisms has been reconsidered as an alternative to antibiotics and chemical preservatives. Herein, inhibition of bacterial adhesion and biofilm formation by anionic chitosan (sulfonated chitosan, SCS) and cationic chitosan (chitosan hydrochloride, WCS) against P. aeruginosa were investigated. Read More

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February 2019

Studies on the molecular dynamics of acetylated oligosaccharides of different topologies (linear versus cyclic).

Carbohydr Polym 2019 Feb 2;206:273-280. Epub 2018 Nov 2.

Silesian Center for Education and Interdisciplinary Research, University of Silesia, ul. 75 Pulku Piechoty 1A, 41-500 Chorzow, Poland; Institute of Physics, University of Silesia, ul. 75 Pulku Piechoty 1, 41-500 Chorzow, Poland.

In this paper, the molecular dynamics and thermal properties of representative acetylated linear and cyclic oligosaccharides: acTRE, acRAF, acSTA, ac-α-CD, ac-β-CD, ac-γ-CD, have been investigated by using broadband dielectric spectroscopy and differential scanning calorimetry. We found that there are marked differences in the dynamics of the structural and secondary relaxation processes in both groups of materials. Just to mention a variation in the distribution of the structural relaxation times as well as different evolutions of the glass transition temperature (T) and fragility (m) versus molecular weight (M), which seem to be affected by the shape of the molecule, strain in the carbohydrate ring and mobility of side acetyl moieties. Read More

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February 2019

Immobilization of hyaluronic acid from Lactococcus lactis on polyethylene terephthalate for improved biocompatibility and drug release.

Carbohydr Polym 2019 Feb 31;206:132-140. Epub 2018 Oct 31.

Department of Biotechnology, Bhupat and Jyoti Mehta School of Biosciences, Indian Institute of Technology Madras, Chennai 600036, India. Electronic address:

Hyaluronic acid from metabolically engineered Lactococcus lactis (HA) was characterized for its biocompatibility and immobilized on the polyethylene terephthalate (PET) surface. HA was chemically crosslinked on hydrolyzed PET (hPET) surface to form HA-coated PET (hPET-HA). The unmodified and modified PET were characterized by Fourier-transform infrared (FT-IR) spectroscopy, scanning electron microscopy (SEM), contact angle measurement, thermogravimetric analysis (TGA), universal testing machine (UTM) and assessed for their biocompatibility. Read More

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February 2019

Characterization and Bifidobacterium sp. growth stimulation of exopolysaccharide produced by Enterococcus faecalis EJRM152 isolated from human breast milk.

Carbohydr Polym 2019 Feb 2;206:102-109. Epub 2018 Nov 2.

Department of Biotechnology, Faculty of Technology, Mahasarakham University, Mahasarakham, 44150, Thailand. Electronic address:

Sixty lactic acid bacteria (LAB) isolated from human breast milk were screened for exopolysaccharides (EPS) production. EPS characterization and Bifidobacterium sp. growth support were investigated. Read More

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February 2019

Fms-like tyrosine kinase 3 is a key factor of male fertility.

Theriogenology 2018 Dec 8;126:145-152. Epub 2018 Dec 8.

Department of Animal Science & Technology, Chung-Ang University, Anseong, Gyeonggi-Do, 17546, Republic of Korea. Electronic address:

Fms-like tyrosine kinase 3 (FLT3) is a type III kinase that is highly expressed in seminal plasma of infertile men. FLT3 activation can be blocked by inhibition of its phosphorylation using the nontoxic and selective inhibitor, quizartinib. We investigated the function of FLT3 and the corresponding effects of quizartinib in mouse spermatozoa. Read More

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December 2018
1 Read

Optimizing denaturing HPLC as a robust technique for identification of Short Tandem Repeats (STR) in forensic medicine.

J Forensic Leg Med 2018 Dec 10;61:108-114. Epub 2018 Dec 10.

Department and Biotechnology Research Center, Semnan University of Medical Sciences, Semnan, Iran. Electronic address:

Introduction: Short Tandem Repeats (STRs) are defined as short lengths of 2-7 base pairs spreading through human genome which due to their highly diverse individually distribution are widely applied for identity detection and other forensic medicine purposes. Burdening considerable costs by the conventional methods such as capillary electrophoresis, we aimed to compare concomitant usage of multiplex PCR and denaturing high-performance liquid chromatography (DHPLC) as cheap, fast, highly accurate, and more accessible methods, with capillary electrophoresis (CE) to evaluate their potential for early screening of STRs.

Materials And Methods: The present study randomly included 20 blood samples from the subjects referred to forensic medicine of Semnan, Iran. Read More

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December 2018
1 Read

Detection of norovirus epidemic genotypes in raw sewage using next generation sequencing.

Environ Int 2018 Dec 12;123:282-291. Epub 2018 Dec 12.

Laboratory of Comparative and Environmental Virology, Oswaldo Cruz Institute, Rio de Janeiro, RJ, Brazil.

Noroviruses are a leading cause of epidemic and pandemic acute gastroenteritis (AGE) worldwide, and contaminated food and water are important routes for its transmission. Raw sewage has been used for viral surveillance to monitor the emergence of new norovirus strains with the potential to cause epidemics. In this study, we investigated norovirus occurrence and norovirus RNA levels in 156 samples collected from May 2013 to May 2014, across three different stages (52 samples each) of a wastewater treatment plant (WWTP) in Rio de Janeiro, Brazil. Read More

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December 2018
2 Reads

Housefly larvae (Musca domestica) significantly accelerates degradation of monensin by altering the structure and abundance of the associated bacterial community.

Ecotoxicol Environ Saf 2018 Dec 12;170:418-426. Epub 2018 Dec 12.

College of Veterinary Medicine, China Agricultural University, No. 2 Yuan Ming Yuan West Road, Hai Dian District, Beijing 100193, China. Electronic address:

Vermicomposting of livestock manure using housefly larvae is a promising biotechnology for waste reduction and control of antibiotic pollution. Monensin (MON), an ionophore polyether antibiotic (IPA), is widely used in broiler feed to control coccidiosis. However, MON residues in litter have become a major source of pollution in the environment. Read More

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December 2018
2 Reads

Two coumarin-based turn-on fluorescent probes based on for hypochlorous acid detection and imaging in living cells.

Spectrochim Acta A Mol Biomol Spectrosc 2018 Dec 10;211:239-245. Epub 2018 Dec 10.

School of Pharmacy, Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection, Yancheng Teachers' University, Yancheng, Jiangsu 224051, People's Republic of China.

This work, two turn-on fluorescent probes (3-acetyl-2H-chromen-2-one (ACO) & (1E)-1-(1-(2-oxo-2H-chromen-3-yl)ethylidene)thiosemicarbazide (CETC)) based on coumarin have been designed and synthesized, which could selectively and sensitively recognize ClO with fast response time. ACO &CETC were almost non fluorescent possibly due to both the lacton form of coumarin and unbridged CN bonds which can undergo a nonradiative decay process in the excited state. Upon the addition of ClO, ACO &CETC were oxidized to ring - opened by cleavage the CO and CN and the fluorescence intensity were increased considerably. Read More

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December 2018

Synthesis of new chalcone-based homoserine lactones and their antiproliferative activity evaluation.

Eur J Med Chem 2018 Dec 7;163:500-511. Epub 2018 Dec 7.

School of Pharmaceutical Sciences, Zhengzhou University, Zhengzhou, 450001, PR China. Electronic address:

Three series of new homoserine lactone analogs were efficiently synthesized starting from methionine and further evaluated for their antiproliferative activity against different cancer cell lines. Among these compounds, some of the chalcone containing compounds 6a-n showed acceptable antiproliferative activity against prostate cancer cells DU145 and PC-3 with the IC values less than 10 μM. Compounds 6c, 6e and 6h inhibited growth of DU145 and PC-3 cells at low micromolar levels with the IC values ranging from 3. Read More

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December 2018
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Successful genetic modification of porcine spermatogonial stem cells via an electrically responsive Au nanowire injector.

Biomaterials 2018 Dec 10;193:22-29. Epub 2018 Dec 10.

Department of Animal Life Science, Kangwon National University, Chuncheon, 24341, South Korea; Department of Applied Animal Science, Kangwon National University, Chuncheon, 24341, South Korea. Electronic address:

Transgenic pigs are quite useful in many biomedical fields, such as xenotransplantation research and the production of biopharmaceutical materials. The genetic transformation of porcine spermatogonial stem cells (pSSCs) followed by differentiation into mature spermatozoa enables the effective production of transgenic pigs. Improving the transfection efficiency of pSSCs, however, has been much desired. Read More

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December 2018

Ergosterol reduction impairs mitochondrial DNA maintenance in S. cerevisiae.

Biochim Biophys Acta Mol Cell Biol Lipids 2018 Dec 12. Epub 2018 Dec 12.

Department of Biology and Biotechnology "Charles Darwin", La Sapienza University of Rome, Piazzale A. Moro 5, 00185 Rome, Italy.; Pasteur Institute-Cenci Bolognetti Foundation, Viale Regina Elena, 291, 00100 Rome, Italy.. Electronic address:

Sterols are essential lipids, involved in many biological processes. In Saccharomyces cerevisiae, the enzymes of the ergosterol biosynthetic pathway (Erg proteins) are localized in different cellular compartments. With the aim of studying organelle interactions, we discovered that Erg27p resides mainly in Lipid Droplets (LDs) in respiratory competent cells, while in absence of respiration, is found mostly in the ER. Read More

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December 2018

Emex spinosa (L.) Campd. ethyl acetate fractions effects on inflammatory mediators and oxidative stressmarkers in carrageenan induced paw oedema in mice.

J Ethnopharmacol 2018 Dec 12. Epub 2018 Dec 12.

Biopesticides Laboratory (LPIP), Center of Biotechnology of Sfax, University of Sfax, P.O. Box. 1177, 3018 Sfax, TUNISIA. Electronic address:

Ethnopharmacological Relevance: Emex spinosa (L.) Campd. (E. Read More

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December 2018
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Human olfactory stem cells: As a promising source of dopaminergic neuron-like cells for treatment of Parkinson's disease.

Neurosci Lett 2018 Dec 12. Epub 2018 Dec 12.

Applied Biophotonics Research Center, Science and Research Branch, Islamic Azad University, Tehran, Iran. Electronic address:

The production of dopaminergic (DA) neurons from stem cells holds a great promise for future clinical treatment of neurodegenerative diseases, such as Parkinson's disease (PD). Olfactory ecto-mesenchymal stem cells (OE-MSCs) derived from the adult human olfactory mucosa can be easily isolated and expanded in culture while maintaining their immense plasticity. In addition to reduced ethical concerns, OE-MSCs could be transplanted across allogeneic barriers, making them desirable stem cells for clinical applications. Read More

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December 2018

Magnetic beads assay based on zip nucleic acid for electrochemical detection of factor V Leiden mutation.

Int J Biol Macromol 2018 Dec 12. Epub 2018 Dec 12.

Faculty of Pharmacy, Analytical Chemistry Department, Ege University, Bornova, Izmir 35100, Turkey; Biotechnology Department, Graduate School of Natural and Applied Sciences, Ege University, Bornova, Izmir 35100, Turkey.

Single nucleotide polymorphisms (SNPs) are the most common type of genetic variation among people. Development of reliable methods for the detection of SNP is crucial in aspects of molecular diagnosis and personalized medicine. In our study, a genomagnetic assay in combination with zip nucleic acid (ZNA) for electrochemical detection of SNP related to Factor V Leiden (FV Leiden) mutation. Read More

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December 2018

Eukaryotic Initiation Factor 4E (eIF4E) sequestration mediates 4E-BP1 response to rapamycin.

Int J Biol Macromol 2018 Dec 12. Epub 2018 Dec 12.

Department of Biotechnology, Science Block, University of Kashmir, Srinagar, J&K 190006, India. Electronic address:

The cap dependent translation initiation is a tightly controlled process of cooperative ternary complex formation by 4E-BP1, eIF4E and the 5' cap of eukaryotic mRNA in response to environmental cues like glucose, nutrients and growth factor levels. Based on the well-described effects of mTORC1/rapamycin complex on 4E-BP1 phosphorylation/s, it is generally accepted that rapamycin is a global inhibitor of cap-dependent translation. We have previously shown that 4E-BP1 resistance to rapamycin was overcome by the stoichiometric abundance of S6K1. Read More

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December 2018
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