5,020 results match your criteria Biotechnology Letters[Journal]


Purification and characterization of glucoamylase of Aspergillus oryzae from Luzhou-flavour Daqu.

Biotechnol Lett 2020 Jul 4. Epub 2020 Jul 4.

College of Bioengineering, Sichuan University of Science & Engineering, No. 180, Xueyuan Street, Huixing Road, Zigong, 643000, Sichuan, People's Republic of China.

Objective: To obtain novel glucoamylase from Daqu microbe.

Results: A dominant strain known as LZ2 with high activity of hydrolyzing starch was isolated from Luzhou Daqu, a Chinese traditional fermentation starter. The LZ2 was identified as Aspergillus oryzae by 18S rDNA sequence analysis. Read More

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http://dx.doi.org/10.1007/s10529-020-02956-4DOI Listing

Improving enzyme activity of glucosamine-6-phosphate synthase by semi-rational design strategy and computer analysis.

Biotechnol Lett 2020 Jun 29. Epub 2020 Jun 29.

State Key Laboratory of Biobased Material and Green Papermaking (LBMP) (Qilu University of Technology), Jinan, 250353, Shandong, People's Republic of China.

Objective: To improve enzyme activity of Glucosamine-6-phosphate synthase (Glms) of Bacillus subtilis by site saturation mutagenesis at Leu, Ala, Lys, Ser and Val based on computer-aided semi-rational design.

Results: The results indicated that LS had the greatest effect on the activity of BsGlms and the enzyme activity increased from 5 to 48 U/mL. The mutation of LS increased the yield of glucosamine by 1. Read More

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http://dx.doi.org/10.1007/s10529-020-02949-3DOI Listing

Inhibition of nattokinase against the production of poly (γ-glutamic Acid) in Bacillus subtilis natto.

Biotechnol Lett 2020 Jun 28. Epub 2020 Jun 28.

College of Biochemical Engineering, Anhui Polytechnic University, Wuhu, Anhui, 241000, People's Republic of China.

Objective: To study the effect of nattokinse (NK) on the synthesis of poly(γ-glutamic acid) (γ-PGA) in Bacillus subtilis natto.

Results: γ-PGA yield significantly decreased as NK was added in the original medium. With the increment of NK dosage, the yield decreased increasingly, but biomass increased instead of decreasing. Read More

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http://dx.doi.org/10.1007/s10529-020-02941-xDOI Listing

Co-evolution of β-glucosidase activity and product tolerance for increasing cellulosic ethanol yield.

Biotechnol Lett 2020 Jun 24. Epub 2020 Jun 24.

College of Life Science, Jiangxi Normal University, Nanchang, 330022, China.

β-Glucosidase (BGL) plays a key role in cellulose hydrolysis. However, it is still a great challenge to enhance product tolerance and enzyme activity of BGL simultaneously. Here, we utilized one round error-prone PCR to engineer the Penicillium oxalicum 16 BGL (16BGL) for improving the cellulosic ethanol yield. Read More

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http://dx.doi.org/10.1007/s10529-020-02935-9DOI Listing

Investigation of the gut microbiome of Apis cerana honeybees from Vietnam.

Biotechnol Lett 2020 Jun 23. Epub 2020 Jun 23.

Molecular Microbiology Lab, Institute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Cau Giay, Hanoi, Vietnam.

Objectives: In this study, the gut microbiome of healthy adult honeybees, Apis cerana, was investigated by sequencing the V3 - V4 region in 16S rRNA gene using Illumina Miseq platform.

Results: The total of 37,853 reads for 16S rRNA gene were obtained and 30,121 (79.6%) reads were valid with 25,291 (84. Read More

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http://dx.doi.org/10.1007/s10529-020-02948-4DOI Listing

Enhancement of S-adenosylmethionine production by deleting thrB gene and overexpressing SAM2 gene in Bacillus amyloliquefaciens.

Biotechnol Lett 2020 Jun 23. Epub 2020 Jun 23.

Key Laboratory of Environment Correlative Dietology (Ministry of Education), College of Food Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China.

Objectives: To improve the S-adenosylmethionine (SAM) production in methionine-free medium, effects of deleting genes of SAM decarboxylase (speD) and homoserine kinase (thrB) on SAM titers were investigated, and the SAM synthetase gene (SAM2) was also overexpressed.

Results: In B. amyloliquefaciens HSAM2, deleting speD to block the SAM utilization pathway significantly reduced the SAM titer. Read More

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http://dx.doi.org/10.1007/s10529-020-02945-7DOI Listing

Aerobic biodegradation of high explosive hexahydro-1,3,5- trinitro-1,3,5-triazine by Janibacter cremeus isolated from contaminated soil.

Biotechnol Lett 2020 Jun 22. Epub 2020 Jun 22.

Delhi Technological University, Delhi, India.

Objective: To evaluate the ability of Janibacter cremeus a soil bacterium isolated from explosive contaminated site in degradation of hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) and to study enzyme responsible for degradation.

Results: The isolate exhibited 88% degradation of RDX in 30 days of incubation. The biodegradation process followed the first order kinetics. Read More

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http://dx.doi.org/10.1007/s10529-020-02946-6DOI Listing

Recombinant ferritin nanoparticles can induce dendritic cell maturation through TLR4/NF-κB pathway.

Biotechnol Lett 2020 Jun 22. Epub 2020 Jun 22.

Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, 600 Changjiang Road, Xiangfang District, Harbin, 150030, Heilongjiang, People's Republic of China.

Objective: Immune response initiation and regulation require activation of dendritic cells (DCs). However, the mechanism by which ferritin, a carrier for immunogen, induces DCs maturation remains unclear.

Results: Recombinant ferritin nanoparticle (RFNp), were prepared through the baculovirus expression vector system, formed spherical and hollow cage-liked proteins with a diameter of approximately 12. Read More

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http://dx.doi.org/10.1007/s10529-020-02944-8DOI Listing

Correction to: Bioconversion of arachidonic acid into human 14,15-hepoxilin B and 13,14,15-trioxilin B by recombinant cells expressing microbial 15-lipoxygenase without and with epoxide hydrolase.

Authors:
J Lee T-H Kim D-K Oh

Biotechnol Lett 2020 Jun 20. Epub 2020 Jun 20.

Department of Bioscience and Biotechnology, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul, 05029, Republic of Korea.

In the original publication of the article, some chirality of Fig. 1 was published incorrectly. The corrected figure is provided below. Read More

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http://dx.doi.org/10.1007/s10529-020-02947-5DOI Listing

By targeting TRAF6, miR-140-3p inhibits TGF-β1-induced human osteosarcoma epithelial-to-mesenchymal transition, migration, and invasion.

Biotechnol Lett 2020 Jun 19. Epub 2020 Jun 19.

Department of Orthopaedics, General Hospital of Tianjin Medical University, 154, Anshan road, Heping district, Tianjin, 300052, China.

Objectives: We evaluated the effects of miR-140-3p on EMT, cellular migration, and invasion in TGF-β1 treated human OS cells. Human fresh OS tissue and normal bone tissue specimens were gathered from 42 patients (29 male and 13 female, 11 to 24 years of age with a mean age of 17.5 ± 2. Read More

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http://dx.doi.org/10.1007/s10529-020-02943-9DOI Listing

Inhibition of berberine hydrochloride on Candida albicans biofilm formation.

Biotechnol Lett 2020 Jun 15. Epub 2020 Jun 15.

College of Medical Technology, Chengdu University of Traditional Chinese Medicine, Sichuan, 611137, China.

Objective: This study aimed to investigate the inhibitory effect of berberine hydrochloride (BH) on Candida albicans (C.albicans) ATCC10231 biofilm formation.

Results: This paper found a positive correlation between the concentration of BH and its inhibitory effect on the cellular activity of early biofilms because we found that 128 and 32 μg/mL BH significantly inhibited biofilm formation (P < 0. Read More

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http://dx.doi.org/10.1007/s10529-020-02938-6DOI Listing
June 2020
1.591 Impact Factor

Purification and characterization of a native lytic polysaccharide monooxygenase from Thermoascus aurantiacus.

Biotechnol Lett 2020 Jun 15. Epub 2020 Jun 15.

Lawrence Berkeley National Laboratory, Joint BioEnergy Institute, Emeryville, CA, 94608, USA.

Lytic polysaccharide monooxygenases (LPMOs) have emerged as key proteins for depolymerization of cellulose. These copper-containing enzymes oxidize C-1 and/or C-4 bonds in cellulose, promoting increased hydrolysis of the oxidized cellulose chains. The LPMO from Thermoascus aurantiacus, a thermophilic ascomycete fungus, has been extensively studied and has served as a model LPMO. Read More

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http://dx.doi.org/10.1007/s10529-020-02942-wDOI Listing

Engineered formate dehydrogenase from Chaetomium thermophilum, a promising enzymatic solution for biotechnical CO fixation.

Biotechnol Lett 2020 Jun 16. Epub 2020 Jun 16.

Department of Bioengineering, Gebze Technical University, 41400, Gebze, Kocaeli, Turkey.

Objectives: Formate dehydrogenases (FDHs) are NAD(P)H-dependent enzymes that catalyse the reversible oxidation of formate to CO. The main goal was to use directed evolution to obtain variants of the FDH from Chaetomium thermophilum (CtFDH) with enhanced reduction activity in the conversion of CO into formic acid.

Results: Four libraries were constructed targeting five residues in the active site. Read More

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http://dx.doi.org/10.1007/s10529-020-02937-7DOI Listing

A novel system to rapidly detect protein-protein interactions (PPIs) based on fluorescence co-localization.

Biotechnol Lett 2020 Jun 13. Epub 2020 Jun 13.

State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, 400716, China.

Objective: Rapid and convenient detection of protein-protein interactions (PPIs) is of great significance for understanding function of protein.

Results: For efficiently detecting PPIs, we used the changes of proteins fluorescence localization to design a novel system, fluorescence translocation co-localization (FTCL), based on nuclear localization signal (NLS) in living cells. Depending on the original state of protein localization (both in the cytoplasm, both in the nucleus, one in the nucleus and another in the cytoplasm), two target proteins can be partitioned into the cytoplasm and nucleus by adding a NLS or mutating an existing NLS. Read More

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http://dx.doi.org/10.1007/s10529-020-02934-wDOI Listing

Production and characterization of an enzyme extract with cellulase activity produced by an indigenous strain of Fusarium verticillioides ITV03 using sweet sorghum bagasse.

Biotechnol Lett 2020 Jun 12. Epub 2020 Jun 12.

Cátedra-CONACYT, Tecnológico Nacional de México/Instituto Tecnológico de Veracruz-UNIDA, Av. M. A. de Quevedo Núm. 2779, C.P. 91860, Veracruz, VER, Mexico.

Objectives: To evaluate a strain of Fusarium verticillioides ITV03 isolated from wood residues in the Veracruz region of Mexico. Endoglucanase and β-glucosidase production by submerged fermentation was optimized using a Box-Behnken design, where the independent variables were urea, ammonium sulfate and yeast extract.

Results: After optimization, an endoglucanase activity of 0. Read More

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http://dx.doi.org/10.1007/s10529-020-02940-yDOI Listing

Overexpression and characterization of a novel GH16 β-agarase (Aga1) from Cellulophaga omnivescoria W5C.

Biotechnol Lett 2020 Jun 9. Epub 2020 Jun 9.

Energy and Environment Fusion Technology Center (E2FTC), Department of Energy Science and Technology (DEST), Myongji University, Yongin-si, Gyeonggi-do, Republic of Korea.

Objective: To identify and characterize a new β-agarase from Cellulophaga omnivescoria W5C capable of producing biologically-active neoagarooligosaccharides from agar.

Results: The β-agarase, Aga1, has signal peptides on both N- and C-terminals, which are involved in the type IX secretion system. It shares 75% protein sequence identity with AgaD from Zobellia galactanivorans and has a molecular weight of 54 kDa. Read More

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http://dx.doi.org/10.1007/s10529-020-02933-xDOI Listing

Improvement of the recombinant human coagulation factor IX expression by co-expression of a novel transcript of Drosophila γ carboxylase in a human cell line.

Biotechnol Lett 2020 Jun 8. Epub 2020 Jun 8.

Department of Molecular Medicine, Institute of Medical Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran.

Objective: Mammalian cells as the main host for production of human proteins are incapable of complete γ-carboxylation of over-expressed Vitamin K Dependent (VKD) proteins. The Drosophila γ-glutamyl carboxylase (DγC) has been shown to be more efficient than its human counterpart in γ-carboxylation of human substrates, in vitro. Considering the Drosophila γ-carboxylase (DγC) efficiency, in comparison with its human counterpart, for recognition and γ-carboxylation of a human substrate in vitro, we were determined to study the effect of the DγC on the hFIX expression in a mammalian cell line. Read More

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http://dx.doi.org/10.1007/s10529-020-02936-8DOI Listing

Neuroprotection of melatonin on spinal cord injury by activating autophagy and inhibiting apoptosis via SIRT1/AMPK signaling pathway.

Biotechnol Lett 2020 Jun 8. Epub 2020 Jun 8.

Department of Orthopedics, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an Jiaotong University, Xi'an, China.

The effect of melatonin (MT) on spinal cord injury (SCI) has attracted increasing research attention. However, the specific role and molecular mechanism of MT on SCI have not been elucidated. An experiment was performed to investigate the effect and molecular mechanism of MT on the neuronal autophagy after SCI and its effect on the recovery of nerve function. Read More

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http://dx.doi.org/10.1007/s10529-020-02939-5DOI Listing

Piperine inhibits colorectal cancer migration and invasion by regulating STAT3/Snail-mediated epithelial-mesenchymal transition.

Biotechnol Lett 2020 Jun 4. Epub 2020 Jun 4.

Department of Gastroenterology, Affiliated Hospital of Guizhou Medical University, Guiyang, 550004, China.

Cancer metastasis is the primary cause of death in patients diagnosed with colorectal cancer. Piperine, an active nontoxic ingredient in pepper, has potent anti-inflammatory and anti-cancer properties. However, little is known about the anti-migratory and anti-invasive effects of piperine on colorectal cancer. Read More

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http://dx.doi.org/10.1007/s10529-020-02923-zDOI Listing

Formaldehyde effects on kanamycin resistance gene of inactivated recombinant Escherichia coli vaccines.

Biotechnol Lett 2020 Jun 4. Epub 2020 Jun 4.

Centro de Desenvolvimento Tecnológico, Biotecnologia, Universidade Federal de Pelotas, Rio Grande do Sul, Pelotas, Brasil.

Objectives: Earlier studies have demonstrated the use of inactivated recombinant E. coli (bacterins), to protect against Clostridium spp. in vaccinated animals. Read More

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http://dx.doi.org/10.1007/s10529-020-02929-7DOI Listing

Efficient CRISPR/Cas9-mediated gene editing in Guangdong small-ear spotted pig cells using an optimized electrotransfection method.

Biotechnol Lett 2020 Jun 3. Epub 2020 Jun 3.

Guangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding, School of Life Science and Engineering, Foshan University, Foshan, 528225, China.

Objectives: Guangdong Small-ear Spotted (GDSS) pigs are a pig breed native to China that possesses unfortunate disadvantages, such as slow growth rate, low lean-meat percentage, and reduced feed utilization. In contrast to traditional genetic breeding methods with long cycle time and high cost, CRISPR/Cas9-mediated gene editing for the modification of the pig genome can quickly improve production traits, and therefore this technique exhibits important potential in the genetic improvement and resource development of GDSS pigs. In the present study, we aimed to establish an efficient CRISPR/Cas9-mediated gene-editing system for GDSS pig cells by optimizing the electrotransfection parameters, and to realize efficient CRISPR/Cas9-mediated gene editing of GDSS pig cells. Read More

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http://dx.doi.org/10.1007/s10529-020-02930-0DOI Listing

Nanoparticle technology improves in-vitro attachment of cattle (Bos taurus) trophectoderm cells.

Biotechnol Lett 2020 Jun 3. Epub 2020 Jun 3.

Department of Animal and Avian Sciences, University of Maryland, College Park, MD, 20742, USA.

The bovine cell line, cow trophectoderm-1 (CT-1), provides an excellent in-vitro cell culture model to study early embryonic development. Obtaining consistent attachment and outgrowth, however, is difficult because enzymatic disassociation into single cells is detrimental; therefore, CT-1 cells must be passaged in clumps, which do not attach readily to the surface of the dish. We tested whether magnetic nanoparticles, NanoShuttle™-PL, could be used to improve cell attachment and subsequent proliferation of the cattle trophectoderm cell line without altering cellular metabolism or immunofluorescent detection of the lineage marker Caudal Type Homeobox 2 (CDX2). Read More

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http://dx.doi.org/10.1007/s10529-020-02926-wDOI Listing

Codon usage trend in genes associated with obesity.

Biotechnol Lett 2020 Jun 1. Epub 2020 Jun 1.

Department of Biomedical Science, Hallym University, Hallymdachak-gil, Chuncheon, 24252, Gangwon-do, Korea.

Obesity is not only a social menace but also an economic burden as it reduces productivity and increases health care cost. We used bioinformatic tools to analyze the CUB of obesity associated genes and compared with housekeeping genes (control) to explore the similarities and differences between two data sets as no work was reported yet. The mean effective number of codons (ENC) in genes associated with obesity and housekeeping gene was 50. Read More

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http://dx.doi.org/10.1007/s10529-020-02931-zDOI Listing

Continuous biomass and lipid production from local chlorella-bacteria consortium in raw wastewater using volatile fatty acids.

Biotechnol Lett 2020 Jun 1. Epub 2020 Jun 1.

Institute of Coastal Agriculture, Hebei Academy of Agriculture and Forestry Sciences, Tangshan Key Laboratory of Plant Salt-Tolerance Research, Tangshan, People's Republic of China.

Objectives: To evaluate mixotrophic cultivation of microalgae-bacteria consortium in raw wastewater by stepwise addition of fermented effluent containing volatile fatty acids (VFAs).

Results: Stepwise increase of VFAs enhanced algal biomass and lipid production, ammonia and phosphate removals. The highest biomass and lipid yield were 1. Read More

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http://dx.doi.org/10.1007/s10529-020-02924-yDOI Listing

Bioconversion of arachidonic acid into human 14,15-hepoxilin B and 13,14,15-trioxilin B by recombinant cells expressing microbial 15-lipoxygenase without and with epoxide hydrolase.

Authors:
J Lee T-H Kim D-K Oh

Biotechnol Lett 2020 Jun 1. Epub 2020 Jun 1.

Department of Bioscience and Biotechnology, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul, 05029, Republic of Korea.

Objective: To produce high concentrations of 13-hydroxy-14,15-epoxy-eicosatrienoic acid (14,15-hepoxilin B, 14,15-HXB) and 13,14,15-trihydroxy-eicosatrienoic acid (13,14,15-trioxilin B, 13,14,15-TrXB) from arachidonic acid (ARA) using microbial 15-lipoxygenase (15-LOX) without and with epoxide hydrolase (EH), respectively.

Results: The products obtained from the bioconversion of ARA by recombinant Escherichia coli cells containing Archangium violaceum 15-LOX without and with Myxococcus xanthus EH were identified as 14,15-HXB and 13,14,15-TrXB, respectively. Under the optimal conditions of 30 g cells L, 200 mM ARA, 25 °C, and initial pH 7. Read More

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http://dx.doi.org/10.1007/s10529-020-02932-yDOI Listing

Improving the thermostability of a GH97 dextran glucosidase by rational design.

Biotechnol Lett 2020 Jun 1. Epub 2020 Jun 1.

School of Life Sciences, Anhui University, Hefei, 230601, Anhui, China.

This study was aimed at improving the thermostability of dextran glucosidase PspAG97A, a member of the glycoside hydrolase family 97, from Pseudoalteromonas sp. K8. A total of 9 lysine residues were chosen using the TKSA-MC program based on the optimization of surface charge-charge interactions and were mutated to glutamate for shifting the enzyme's isoelectric point off its optimum pH value. Read More

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http://dx.doi.org/10.1007/s10529-020-02928-8DOI Listing
June 2020
1.591 Impact Factor

MicroRNA miR-212 regulates PDCD4 to attenuate Aβ-induced neurotoxicity via PI3K/AKT signaling pathway in Alzheimer's disease.

Biotechnol Lett 2020 May 30. Epub 2020 May 30.

Department of Neurology, The Second Hospital of Dalian Medical University, No. 467 Zhongshan Rd, Shahekou District, Dalian, 116023, China.

Background: Alzheimer's disease (AD) is a progressive neurodegenerative disease in the elderly. MicroRNA (miRNA) miR-212-3p (miR-212) has been reported to dysregulated in many neurodegenerative diseases including AD. However, the mechanism and function of miR-212 in AD has not been reported. Read More

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http://dx.doi.org/10.1007/s10529-020-02915-zDOI Listing

Proteomic analysis reveals the damaging role of low redox laccase from Yersinia enterocolitica strain 8081 in the midgut of Helicoverpa armigera.

Biotechnol Lett 2020 May 29. Epub 2020 May 29.

Laboratory of Enzymology and Recombinant DNA Technology, Department of Microbiology, Maharshi Dayanand University, Rohtak, Haryana, 124001, India.

Objective: Earlier, we have found that the enteropathogenic Yersinia enterocolitica have evolved the survival mechanisms that regulate the expression of laccase-encoding genes in the gut. The present study aims to characterize the purified recombinant laccase from Y. enterocolitica strain 8081 biovar 1B and understand its effect on the midgut of cotton bollworm, Helicoverpa armigera (Hübner) larvae. Read More

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http://dx.doi.org/10.1007/s10529-020-02925-xDOI Listing
May 2020
1.591 Impact Factor

Marine bacterial community analysis on 316L stainless steel coupons by Illumina MiSeq sequencing.

Biotechnol Lett 2020 May 29. Epub 2020 May 29.

Microbial Corrosion Laboratory, Estácio University (UNESA), Bispo Street, 83, Room, AG405, Rio de Janeiro, Rio de Janeiro, ZIP Code 20261-063, Brazil.

In order to evaluate the corrosive action of microorganisms on 316L metal exposed directly to a marine environment, a system was designed to immerse coupons in seawater. After periods of 30, 60 and 90 days, the coupons were recovered, the corrosion rates evaluated and the biofilm samples on their surface were analyzed by 16S rRNA gene sequencing. The results of the corrosion rate showed an acceleration over the entire experimental period. Read More

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http://dx.doi.org/10.1007/s10529-020-02927-9DOI Listing

Comparative evaluation of silver nanoparticles biosynthesis by two cold-tolerant Streptomyces strains and their biological activities.

Biotechnol Lett 2020 May 27. Epub 2020 May 27.

Department of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran.

The present study reflected on high-priority biological activities of novel silver nanoparticles (AgNPs) synthesized via two cold-tolerant strains; namely, Streptomyces sp.OSIP1 and Streptomyces sp.OSNP14. Read More

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http://dx.doi.org/10.1007/s10529-020-02921-1DOI Listing

The synergy between LPMOs and cellulases in enzymatic saccharification of cellulose is both enzyme- and substrate-dependent.

Biotechnol Lett 2020 May 26. Epub 2020 May 26.

Department of Plant and Environmental Sciences, University of Copenhagen, 1871, Frederiksberg, Denmark.

Objectives: The synergistic effects between cellulases and lytic polysaccharide monooxygenases (LPMOs) were investigated systematically in terms of their degree of synergy (DS) on amorphous and crystalline cellulose. Synergy curves were obtained for enzyme pairs containing a cellulase from Trichoderma reesei (Cel6A and Cel7A) and three LPMOs from Thermoascus aurantiacus (TaAA9A), Lentinus similis (LsAA9A) and Thielavia terrestris (TtAA9E).

Results: The synergistic experiments showed that the three LPMOs significantly improved the hydrolytic efficiency of Cel6A, on both cellulosic substrates; a more pronounced effect being seen for TtAA9E on amorphous cellulose at low cellulase:LPMO ratios. Read More

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http://dx.doi.org/10.1007/s10529-020-02922-0DOI Listing

Optimization for allitol production from D-glucose by using immobilized glucose isomerase and recombinant E. coli expressing D-psicose-3-epimerase, ribitol dehydrogenase and formate dehydrogenase.

Biotechnol Lett 2020 May 25. Epub 2020 May 25.

State Key Laboratory of Microbial Technology, Shandong University (Qingdao), Qingdao, China.

Objective: To develop a method combining enzymatic catalysis and resting-cell biotransformation to produce allitol from low cost substrate D-glucose.

Results: The recombinant E. coli expressing D-psicose-3-epimerase (DPE), ribitol dehydrogenase (RDH) and formate dehydrogenase (FDH) for allitol production from D-fructose was constructed. Read More

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http://dx.doi.org/10.1007/s10529-020-02917-xDOI Listing

Single-nucleotide polymorphisms influence the promoter activities of systemic lupus erythematosus-associated genes.

Biotechnol Lett 2020 May 25. Epub 2020 May 25.

Department of Laboratory Medicine, Pingshan District People's Hospital, Shenzhen, 518118, Guangdong, China.

Systemic lupus erythematosus (SLE) is a protypical autoimmune disease and genetic factors play important roles in its pathogenesis. Since present SLE susceptibility loci are mainly studied through meta-analysis of genome-wide association study, we performed promoter activity analysis to examine the biological functions of SLE-associated single-nucleotide polymorphisms (SNPs). We found at SNP positions rs1341239, rs1800795, rs1800796, rs1800872, rs1800871, rs187238, rs360719, rs8178822, rs3761549, different alleles influenced respective promoter activities in different manners, and the effects also appeared under glucocorticoid treatment. Read More

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http://dx.doi.org/10.1007/s10529-020-02916-yDOI Listing
May 2020
1.591 Impact Factor

Mechanical loading induces HIF-1α expression in chondrocytes via YAP.

Biotechnol Lett 2020 May 25. Epub 2020 May 25.

Department of Spine Surgery, Shandong Provincial Hospital affiliated to Shandong First Medical University, 324 Jingwuweiqi Road, Jinan, Shandong, China.

Objectives: To investigate the role of YAP in cyclic mechanical stress induced up-regulation of HIF-1α in rat cartilage chondrocytes.

Results: Our in vitro and in vivo experiments demonstrated that cyclic mechanical stress promoted HIF-1α and YAP proteins expression in a magnitude dependent manner. Cyclic mechanical stress at 4000μ strain exhibited most significant effect in promoting HIF-1α and YAP up-regulation. Read More

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http://dx.doi.org/10.1007/s10529-020-02910-4DOI Listing

Inhibition of Salmonella enteritidis biofilms by Salmonella invasion protein-targeting aptamer.

Biotechnol Lett 2020 May 25. Epub 2020 May 25.

Basic and Industrial Microbiology Section, Biology Department, Faculty of Science, Ege University, 35100, Izmir, Turkey.

The current study aimed to assess the inhibitory effect of a DNA aptamer (Apt17) which targeted Salmonella invasion proteinA (SipA). The effect of Apt17, on biofilm formation by two Salmonella enteritidis strains, was tested either separately or in combination with ampicillin at different Sub MIC concentrations. Maximum inhibitory effect equivalent to 24. Read More

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http://dx.doi.org/10.1007/s10529-020-02920-2DOI Listing

Effect of a novel postbiotic containing lactic acid bacteria on the intestinal microbiota and disease resistance of rainbow trout (Oncorhynchus mykiss).

Biotechnol Lett 2020 May 25. Epub 2020 May 25.

Instituto Agroalimentario de Aragón (IA2), CITA - Universidad de Zaragoza, Zaragoza, Spain.

Objective: This study was aimed to assess the effect of a novel postbiotic on bacterial community composition and structure within the intestinal ecosystem of rainbow trout (Oncorhynchus mykiss), as well as evaluate its capacity to protect rainbow trout from Lactococcus garvieae infection.

Results: After 30 days of dietary postbiotic supplementation, high-throughput 16S rRNA gene sequencing revealed that bacterial community composition, diversity and richness were significantly higher in treated fish than in control fish. The proportion of sequences affiliated to the phylum Tenericutes, and to a lesser extent, the phyla Spirochaetes and Bacteroidetes was increased in fish fed a postbiotic-enriched diet compared to control fish, whereas the abundance of Fusobacteria was higher in control fish. Read More

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http://dx.doi.org/10.1007/s10529-020-02919-9DOI Listing

B1 protein: a novel cell penetrating protein for in vitro and in vivo delivery of HIV-1 multi-epitope DNA constructs.

Biotechnol Lett 2020 May 25. Epub 2020 May 25.

Department of Pharmaceutical Biotechnology, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Objectives: Enhancement of the potential ability of biomacromolecules to cross cell membranes is a critical step for development of effective therapeutic vaccine especially DNA vaccine against human immunodeficiency virus-1 (HIV-1) infection. The supercharged proteins were known as powerful weapons for delivery of different types of cargoes such as DNA and protein. Hence, we applied B1 protein with + 43 net charges obtained from a single frameshift in the gene encoding enhanced green fluorescent protein (eGFP) for delivery of two multi-epitope DNA constructs (nef-vpu-gp160-p24 and nef-vif-gp160-p24) in vitro and in vivo for the first time. Read More

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http://dx.doi.org/10.1007/s10529-020-02918-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7246087PMC

MicroRNA-191 regulates differentiation and migration of mesenchymal stem cells and their paracrine effect on angiogenesis.

Biotechnol Lett 2020 May 20. Epub 2020 May 20.

School of Chemistry, Chemical Engineering and Life Sciences, Wuhan University of Technology, Wuhan, 430070, Hubei Province, China.

MicroRNAs (miRNAs) are critical regulators in organ development. Among them, miR-191 is known to be regulated in early embryogenesis and dysregulated in cancer. This role in undifferentiated tissues suggests a possible part of miR-191 also in bone marrow derived mesenchymal stem cells (BMSCs) physiology. Read More

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http://dx.doi.org/10.1007/s10529-020-02907-zDOI Listing

Internalization of the TAT-PBX1 fusion protein significantly enhances the proliferation of human hair follicle-derived mesenchymal stem cells and delays their senescence.

Biotechnol Lett 2020 May 20. Epub 2020 May 20.

Department of Toxicology, School of Public Health, Jilin University, 1163 Xinmin Avenue, Changchun, 130021, China.

Objectives: To express a TAT-PBX1 fusion protein using a prokaryotic expression system and to explore potential effects of TAT-PBX1 in the proliferation and senescence of human hair follicle-derived mesenchymal stem cells.

Results: The TAT-PBX1 fusion was produced in inclusion bodies and heterogenously expressed in Rosetta (DE3) cells. Immunofluorescence staining showed that TAT-PBX1 fusion proteins were internalized by human hair follicle-derived mesenchymal stem cells. Read More

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http://dx.doi.org/10.1007/s10529-020-02909-xDOI Listing

Small design from big alignment: engineering proteins with multiple sequence alignment as the starting point.

Biotechnol Lett 2020 May 19. Epub 2020 May 19.

College of Physics and Electronic Engineering, Xinyang Normal University, Xinyang, 464000, China.

Multiple sequence alignment (MSA) is a fundamental way to gain information that cannot be obtained from the analysis of any individual sequence included in the alignment. It provides ways to investigate the relationship between sequence and function from a perspective of evolution. Thus, the MSA of proteins can be employed as a reference for protein engineering. Read More

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http://dx.doi.org/10.1007/s10529-020-02914-0DOI Listing

The effect of exosomes derived from mesenchymal stem cells in the treatment of induced type 1 diabetes mellitus in rats.

Biotechnol Lett 2020 May 19. Epub 2020 May 19.

Medical Biochemistry and Molecular Biology, Faculty of Medicine, Cairo University, Giza, Egypt.

Aim: The aim of the current study was to evaluate the therapeutic and regenerative effects of MSCs derived exosomes in the treatment of type 1 DM and to compare its effects with MSCs themselves. The experiment was done on forty albino rats grouped as follows, group (1): Ten healthy rats, group (2): Ten induced type 1 DM rats, group (3): Ten induced type 1 DM rats received exosomes intraperitoneally, and group (4): Ten induced type 1 DM rats received MSCs intraperitoneally. Serum glucose and plasma insulin levels were assessed weekly. Read More

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http://dx.doi.org/10.1007/s10529-020-02908-yDOI Listing
May 2020
1.591 Impact Factor

Enhanced production of Aspergillus niger inulinase from sugar beet molasses and its kinetic modeling.

Biotechnol Lett 2020 May 18. Epub 2020 May 18.

Department of Food Engineering, Akdeniz University, 07058, Antalya, Turkey.

Objective: The fermentation medium contains many complex components (vitamins, minerals, etc.) for better growth of the microorganisms. The increasing purity and number of these components used in the medium seriously affect the cost of the microbial process. Read More

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http://dx.doi.org/10.1007/s10529-020-02913-1DOI Listing

Soybean peptides promote yoghurt fermentation and quality.

Biotechnol Lett 2020 May 17. Epub 2020 May 17.

Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Engineering and Technology Research Center of Food Additives, Beijing Technology and Business University, Beijing, China.

Objectives: This research paper was to investigate the influence of soybean peptides addition on viable count of lactic acid bacteria, physicochemical parameters, flavor, and sensory evaluation of yoghurt.

Results: The number of fermenting strains (Streptococcus thermophilus + Lactobacillus delbrueckii subsp. bulgaricus) cells in yoghurt (stored at 4 °C for 19 days) added with 0. Read More

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http://dx.doi.org/10.1007/s10529-020-02912-2DOI Listing

Recombinant expression of sericin-cecropin fusion protein and its functional activity.

Biotechnol Lett 2020 May 16. Epub 2020 May 16.

Seri-biotech Research Laboratory, Central Silk Board, Kodathi, Carmelaram Post, Bengaluru, 560 035, India.

Objective: Silk sericin is a natural polymer with potential utility in biomedical and biotechnological applications. Recombinantly expressed sericin ensures a source of pure protein with no contamination and with multiple properties when expressed as a fusion protein. Hence, the present paper aims to recombinantly express a functional silk sericin fusion protein. Read More

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http://dx.doi.org/10.1007/s10529-020-02911-3DOI Listing

Characterization of the hydrolysate and catalytic cavity of α-agarase AgaD.

Biotechnol Lett 2020 May 13. Epub 2020 May 13.

School of Medicine and Pharmacy, Key Laboratory of Marine Drugs, Chinese Ministry of Education, Key Laboratory of Glycoscience & Glycotechnology of Shandong Province, Ocean University of China, Qingdao, 266003, China.

Objective: To characterize the hydrolysis product and the substrate binding in the catalytic cavity of α-agarase AgaD.

Results: The time course curve showed that AgaD degraded agarose by the endo-type cleavage. AgaD did not degrade agarobiose (A2) and agarotetraose (A4). Read More

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http://dx.doi.org/10.1007/s10529-020-02901-5DOI Listing
May 2020
1.591 Impact Factor

The immune response to a recombinant Lactococcus lactis oral vaccine against foot-and-mouth disease virus in mice.

Biotechnol Lett 2020 May 9. Epub 2020 May 9.

State Key Laboratory of Veterinary Etiological Biology, National Foot-and-Mouth Disease Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu, China.

Objective: Development of an effective mucosal vaccine to induce specific immune responses against Foot-and-mouth disease virus (FMDV).

Results: For this purpose, the FMDV VP1 gene (SPVP1) was optimized and synthesized based on the codon bias of Lactococcus lactis (L. lactis), and then incorporated in the plasmid pNZ8148. Read More

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http://dx.doi.org/10.1007/s10529-020-02900-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7210100PMC

PPARα plays an important role in the migration activity, and the expression of CYP2S1 and CYP1B1 in chrysin-treated HCT116 cells.

Biotechnol Lett 2020 May 8. Epub 2020 May 8.

Institute for Research in Molecular Medicine (INFORMM), Universitit Sains Malaysia, 11800, Penang, Malaysia.

Objective: This study aimed to examine the metabolising effect of chrysin by investigating the mRNA expression levels of PPARα and its related cellular mechanisms in HCT116 cells.

Results: The mRNA expression of PPARα was significantly induced in HCT116 cells following treatment with chrysin for 36 h, but the mRNA expression of PPARα was inhibited, when the cells were treated with a combination of chrysin and MK886 (PPARα inhibitor). This phenomenon proved that the incorporation of MK886 lowers the expression levels of PPARα, thus enabling us to study the function of PPARα. Read More

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http://dx.doi.org/10.1007/s10529-020-02904-2DOI Listing

MicroRNA-127-3p regulates myoblast proliferation by targeting Sept7.

Biotechnol Lett 2020 May 7. Epub 2020 May 7.

Herbivorous Livestock Institute, Chongqing Academy of Animal Sciences, Chongqing, 402460, China.

Objective: MicroRNAs (miRNAs) are highly conserved, endogenous small RNAs that regulate gene expression at the post-transcriptional level. miR-127 plays an essential role in myogenic differentiation in vivo and in vitro. However, it is not clear whether miR-127-3p affects myogenic cell proliferation. Read More

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http://dx.doi.org/10.1007/s10529-020-02906-0DOI Listing
May 2020
1.591 Impact Factor

Functional variations between Mesenchymal Stem Cells of different tissue origins: A comparative gene expression profiling.

Biotechnol Lett 2020 Jul 5;42(7):1287-1304. Epub 2020 May 5.

Hepato-Pancreato-Biliary Centre for Surgery & Transplantation, MIOT International, Chennai, Tamilnadu, India.

Background: Mesenchymal Stem Cells (MSCs), regardless of the tissue sources, are considered as excellent candidates for cellular therapy as they are immune-privileged cells containing a multitude of therapeutic functions that aid in tissue regeneration and repair. For the effective application of these cells in cell therapy, it is important to understand and characterize their biological functions.

Objectives: The present study attempts to characterize the variations in multipotent function such as cell surface antigen levels, proliferation, differentiation and stemness (pluripotency) potential of MSCs isolated from foetal [wharton's jelly (WJ), foetal and maternal side of placenta (PF and PM)] and adult tissue sources [bone marrow (BM) and adipose tissue (AT)] using gene expression by real time PCR (qRT-PCR). Read More

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http://dx.doi.org/10.1007/s10529-020-02898-xDOI Listing

Construction of a system for single-stranded DNA isolation.

Biotechnol Lett 2020 May 5. Epub 2020 May 5.

School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, China.

Objective: The system of Strep-Tactin and StrepII tag-SSB proteins binding (ST-SSB) was established to isolate the purified single-stranded DNA in a single step with low cost and high efficiency.

Results: We demonstrate that in the presence of large amounts of dsDNA, the ssDNA binding specificity of Escherichia coli (E. coli) single stranded DNA binding (EcSSB) protein was stronger than gene-5-protein (g5p). Read More

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http://dx.doi.org/10.1007/s10529-020-02905-1DOI Listing