4,749 results match your criteria Biotechnology Letters[Journal]


Recent advances in carbohydrate-based cancer vaccines.

Biotechnol Lett 2019 Apr 16. Epub 2019 Apr 16.

Key Laboratory of Tumor Molecular Diagnosis and Individualized Medicine of Zhejiang Province, Zhejiang Provincial People's Hospital (People's Hospital of Hangzhou Medical College), Hangzhou, 310014, Zhejiang, People's Republic of China.

Cancer is a complex multifactorial disease for which many promising therapeutic strategies such as immunotherapy are emerging. Malignant cells frequently express aberrant cell surface carbohydrates, which differentiate them from normal "healthy" cells. This characteristic presents a window for the development of synthetic carbohydrate antigen-based cancer vaccines which can be recognized by the immune system and can bring about T cell-dependent immune responses. Read More

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http://dx.doi.org/10.1007/s10529-019-02675-5DOI Listing

Lipid production from indigenous Greek microalgae: a possible biodiesel source.

Biotechnol Lett 2019 Apr 16. Epub 2019 Apr 16.

Microbiology Group, Sector of Botany, Department of Biology, National and Kapodistrian University of Athens, Zografou Campus, 15781, Athens, Greece.

Objective: Microalgae gained interest for potential use as biodiesel producers, since they synthesize and accumulate significant quantities of lipids. The aim of this work was to isolate indigenous microalgae strains from Greek habitats, study their physicochemical growth conditions and finally select the best ones with respect to overall lipid production and profile.

Results: Two sampling sites of marine aquatic ecosystems were selected in Attica prefecture, Greece in order to screen for novel wild type strains with lipid production capacity. Read More

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http://link.springer.com/10.1007/s10529-019-02658-6
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http://dx.doi.org/10.1007/s10529-019-02658-6DOI Listing
April 2019
1 Read

Enzymatic transformation of ginsenosides Re, Rg1, and Rf to ginsenosides Rg2 and aglycon PPT by using β-glucosidase from Thermotoga neapolitana.

Biotechnol Lett 2019 Apr 9. Epub 2019 Apr 9.

College of Food Science and Engineering, Jilin Agriculture University, Changchun, 130118, Jilin, China.

Objectives: To enzymatically transform protopanaxatriol by using β-glucosidase from Thermotoga neapolitana (T. neapolitana) DSM 4359.

Results: Recombinant β-glucosidase was purified, which molecular weight was about 79. Read More

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http://dx.doi.org/10.1007/s10529-019-02665-7DOI Listing

Online monitoring of the redox potential in microaerobic and anaerobic Scheffersomyces stipitis fermentations.

Biotechnol Lett 2019 Apr 8. Epub 2019 Apr 8.

Chemical Engineering and Food Engineering Department - Santa Catarina Federal University, Florianópolis, SC, Zip Code 88040-900, Brazil.

Objective: A correlation among different volumetric oxygen transfer coefficients (ka) and the oxireduction potential (ORP) in batch fermentations using Scheffersomyces stipitis was evaluated. Experiments were performed using a mixture of xylose and glucose as the substrates.

Results: Microaerophilic condition (ka = 4. Read More

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http://dx.doi.org/10.1007/s10529-019-02674-6DOI Listing

A cell ELISA based method for exosome detection in diagnostic and therapeutic applications.

Biotechnol Lett 2019 Apr 8. Epub 2019 Apr 8.

Department of Medical Biotechnology, Faculty of Medical Sciences, Tarbiat Modares University, Intersection of Chamran and AL-E-Ahmad Highways, P.O. Box: 14115-111, Tehran, Iran.

Objective: Exosomes are nano-scaled carriers of miRNA, mRNA and proteins which are secreted from viable cells. Exosome detection within serum, saliva and semen offers diagnostic value for detection of various diseases including cancer. In the present study, we have lunched an in vitro study to develop a more efficient method for exosome detection. Read More

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http://link.springer.com/10.1007/s10529-019-02667-5
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http://dx.doi.org/10.1007/s10529-019-02667-5DOI Listing
April 2019
4 Reads

Human genome-derived TOP1 matrix attachment region enhances transgene expression in the transfected CHO cells.

Biotechnol Lett 2019 Apr 5. Epub 2019 Apr 5.

International Joint Research Laboratory for Recombiant Pharmaceutical Protein Expression System of Henan, Xinxiang Medical University, Xinxiang, 453003, Henan, China.

Objectives: To investigate the effect of full-length fragment of DNA topoisomerase I gene (TOP1) matrix attachment regions (MARs) originating from the human genome on transgene expression in Chinese hamster ovary (CHO) cells and explore the underlying mechanisms.

Results: Results showed that TOP1 MAR cannot only enhance the transient and stable transgenic expression of enhanced green fluorescence protein (EGFP) but also increase long-term stability and ratio of positive colonies in transfected CHO cells with TOP1 MAR at the 5' or 3' ends of the EGFP expression cassette. Interestingly, the CHO cells were transfected with the 5',3' TOP1 MAR-containing vector featured the highest transient and stable expression, whereas those with the 3' TOP1 MAR-containing vector exhibited the most effective stability and ratio of positive colonies. Read More

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http://dx.doi.org/10.1007/s10529-019-02673-7DOI Listing

Theophylline-inducible riboswitch accurately regulates protein expression at low level in Escherichia coli.

Biotechnol Lett 2019 Apr 5. Epub 2019 Apr 5.

Department of Bioinformatic Engineering, Graduate School of Information Science and Technology, Osaka University, 1-5 Yamadaoka, Suita, Osaka, 565-0871, Japan.

Objectives: Fine-tuning of enzyme expression at low levels is an important challenge for metabolic engineers. Here, theophylline-inducible riboswitch for translational regulation was evaluated. The background expression, translation rate, and time delay for its induction was reported. Read More

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http://dx.doi.org/10.1007/s10529-019-02672-8DOI Listing

Single chamber air-cathode microbial fuel cells as biosensors for determination of biodegradable organics.

Biotechnol Lett 2019 Apr 3. Epub 2019 Apr 3.

Department of Applied Biotechnology and Food Science, Budapest University of Technology and Economics, Szt. Gellért tér 4, Budapest, 1111, Hungary.

Objectives: Single chamber air cathode microbial fuel cells (MFCs) were investigated with sodium-acetate and peptone as test substrates to assess the potential for application as biosensor to determine the concentration of biodegradable organics in water/wastewater samples.

Results: MFCs provided well-reproducible performance at high (> 2000 mg COD l-Chemical Oxygen Demand) acetate concentration values. Current in the cells proved to be steady from 25 to 35 °C, significant decrease was, however, revealed in the current below 20 °C. Read More

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http://dx.doi.org/10.1007/s10529-019-02668-4DOI Listing
April 2019
2 Reads

Expression and hydroxyurea-triggered induction of EGFP upon CRISPR/Cas9-mediated integration into the γ-globin gene of K562 cells.

Biotechnol Lett 2019 Apr 2. Epub 2019 Apr 2.

Genetics Research Center, University of Social Welfare and Rehabilitation Sciences, Evin, Daneshjoo Blvd., Koodakyar St., Tehran, 1985713834, Iran.

Objective: To knock-in an EGFP cassette into the γ-globin genes of K562 cells via CRISPR/Cas9, and to assess expression and hydroxyurea (HU)-mediated induction of the targeted EGFP transgene.

Results: The EGFP cassettes were specifically knocked into the Gγ gene. EGFP expression was detected in the targeted cell population and isolated clones. Read More

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http://dx.doi.org/10.1007/s10529-019-02671-9DOI Listing
April 2019
1 Read

Construction of a novel bioanode for amino acid powered fuel cells through an artificial enzyme cascade pathway.

Biotechnol Lett 2019 Apr 1. Epub 2019 Apr 1.

Division of Engineering, Faculty of Engineering, University of Fukui, 3-9-1 Bunkyo, Fukui, 910-8507, Japan.

Objective: The construction of a novel bioanode based on L-proline oxidation using a cascade reaction pathway comprised of thermostable dehydrogenases.

Results: A novel multi-enzymatic cascade pathway, containing four kinds of dehydrogenases from thermophiles (dye-linked L-proline dehydrogenase, nicotinamide adenine dinucleotide (NAD)-dependent Δ-pyrroline-5-carboxylate dehydrogenase, NAD-dependent L-glutamate dehydrogenase and dye-linked NADH dehydrogenase), was designed for the generation of six-electrons from one molecule of L-proline. The current density of the four-dehydrogenase-immobilized electrode, with a voltage of + 450 mV (relative to that of Ag/AgCl), was 226. Read More

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http://dx.doi.org/10.1007/s10529-019-02664-8DOI Listing

Mild heat stress limited the post-acidification caused by Lactobacillus rhamnosus hsryfm 1301 in fermented milk.

Biotechnol Lett 2019 Mar 30. Epub 2019 Mar 30.

College of Food Science and Engineering, Yangzhou University, 196 Huayang Xilu, Yangzhou, 225100, People's Republic of China.

Objective: Fermented milk is the optimal vehicle for delivering probiotic bacteria. However, the viable count of probiotic bacteria such as some lactic acid bacteria and the post-acidification of fermented milk are a contradiction. The objective of this study was to restrict the post-acidification of the fermented milk containing living Lactobacillus rhamnosus hsryfm 1301. Read More

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http://dx.doi.org/10.1007/s10529-019-02669-3DOI Listing

Mupirocin: applications and production.

Biotechnol Lett 2019 Mar 29. Epub 2019 Mar 29.

Faculty of Chemical Engineering and Environmental Protection "Cristofor Simionescu", "Gheorghe Asachi" Technical University of Iasi, Iasi, Romania.

Mupirocin is an antibiotic from monocarboxylic acid class used as antibacterial agent against methicillin-resistant Staphylococcus aureus (MRSA) and can be obtained as a mixture of four pseudomonic acids by Pseudomonas fluorescens biosynthesis. Nowadays improving antibiotics occupies an important place in the pharmaceutical industry as more and more resistant microorganisms are developing. Mupirocin is used to control the MRSA outbreaks, for infections of soft tissue or skin and for nasal decolonization. Read More

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http://dx.doi.org/10.1007/s10529-019-02670-wDOI Listing

Thermostable CITase from Thermoanaerobacter thermocopriae shows negative cooperativity.

Biotechnol Lett 2019 Mar 29. Epub 2019 Mar 29.

Department of Food Science and Technology, Chonnam National University, Gwangju, 61186, Republic of Korea.

Objective: The biochemical properties of a putative thermostable cycloisomaltooligosaccharide (CI) glucanotransferase gene from Thermoanaerobacter thermocopriae were determined using a recombinant protein (TtCITase) expressed in Escherichia coli and purified to a single protein.

Results: The 171-kDa protein displayed maximum activity at pH 6.0, and enzyme activity was stable at pH 5. Read More

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http://dx.doi.org/10.1007/s10529-019-02666-6DOI Listing

Biodesulfurization of diesel oil in oil-water two phase reaction system by Gordonia sp. SC-10.

Biotechnol Lett 2019 Mar 26. Epub 2019 Mar 26.

College of Chemical Engineering, China University of Petroleum (East China), Qingdao, 266580, People's Republic of China.

Objectives: Different sulfur contents of diesel oils were used for biodesulfurization to study the desulfurization capacity of Gordonia sp. SC-10 in oil-water two-phase reaction system.

Results: Gordonia sp. Read More

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http://dx.doi.org/10.1007/s10529-019-02663-9DOI Listing

A novel SGNH family hydrolase Ali5 with thioesterase activity and a GNSL motif but without a classic GDSL motif from Altererythrobacter ishigakiensis.

Biotechnol Lett 2019 Mar 20. Epub 2019 Mar 20.

Key Laboratory of Marine Ecosystem and Biogeochemistry, State Oceanic Administration, Hangzhou, 310012, People's Republic of China.

Objective: We aimed to characterize a novel SGNH (Ser-Gly-Asn-His) family hydrolase from the annotated genome of marine bacteria with new features.

Results: A novel esterase Ali5 from Altererythrobacter ishigakiensis has been identified and classified into SGNH family. Ali5 presented a novel GNSL (Gly-Asn-Ser-Leu(X)) motif that differs from the classic GDSL (Gly-Asp-Ser-Leu(X)) motif of SGNH family. Read More

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http://dx.doi.org/10.1007/s10529-019-02662-wDOI Listing

Cloning, expression, and characterization of a novel nitrilase, PaCNit, from Pannonibacter carbonis Q4.6.

Biotechnol Lett 2019 Mar 19. Epub 2019 Mar 19.

State Key Laboratory of Heavy Oil Processing and Centre for Bioengineering and Biotechnology, College of Chemical Engineering, China University of Petroleum (East China), Qingdao, 266580, China.

Objective: Identification of a heavy metal ion-stimulated nitrilase with broad-spectrum substrate specificity.

Results: A novel nitrilase, PaCNit, was identified from Pannonibacter carbonis Q4.6 and its enzymatic properties were investigated. Read More

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http://dx.doi.org/10.1007/s10529-019-02661-xDOI Listing

Electrospun essential oil-polycaprolactone nanofibers as antibiofilm surfaces against clinical Candida tropicalis isolates.

Biotechnol Lett 2019 Mar 16. Epub 2019 Mar 16.

Biotechnology Division, Biology Department, Faculty of Sciences, Hacettepe University, 06800, Beytepe, Ankara, Turkey.

Objective: As an approach to prevent biofilm infections caused by Candida tropicalis on various surfaces, determination of effect of biodegradable polycaprolactone nanofibers (PCLNFs) with different concentrations of two different essential oils were tested in this study.

Results: Both of the tested essential oils exhibited antifungal effect (minimal inhibitory concentration; 0.25-0. Read More

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http://dx.doi.org/10.1007/s10529-019-02660-yDOI Listing

Production of 8S- and 10S-hydroxy polyunsaturated fatty acids by recombinant Escherichia coli cells expressing mouse arachidonate 8S-lipoxygenase.

Biotechnol Lett 2019 Mar 2. Epub 2019 Mar 2.

Department of Bioscience and Biotechnology, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul, 05029, Republic of Korea.

Objective: To quantitatively hydroxylate 8S- and 10S-positions on polyunsaturated fatty acids by recombinant Escherichia coli cells expressing mouse arachidonate 8S-lipoxygenase (8S-LOX).

Results: Hydroxylated products gained from the conversion of arachidonic acid (20:4, AA), eicosapentanoic acid (20:5, EPA), and (22:6, DHA) by recombinant E. coli cells containing 8S-LOX from mouse were identified as 8S-hydroxy-5,9,11,14(Z,E,Z,Z)-eicosatetranoic acid (8S-HETE), 8S-hydroxy-5,9,11,14,17(Z,E,Z,Z,Z)-eicosapentanoic acid (8S-HEPE), and 10S-hydroxy-4,8,12,14,16,19(Z,E,Z,Z,Z,Z)-docosahexaenoic acid (10S-HDoHE), respectively. Read More

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http://dx.doi.org/10.1007/s10529-019-02659-5DOI Listing

Meeting the challenge of developing food crops with improved nutritional quality and food safety: leveraging proteomics and related omics techniques.

Biotechnol Lett 2019 Feb 28. Epub 2019 Feb 28.

School of Life Sciences, Jawaharlal Nehru University, New Delhi, India.

Eliminating malnutrition remains an imminent priority in our efforts to achieve food security and providing adequate calories, proteins, and micronutrients to the growing world population. Malnutrition may be attributed to socio-economic factors (poverty and limited accessibility to nutritional food), dietary preferences, inherent nutrient profiles of traditional food crops, and to a combination of all such factors. Modern advancements in "omics" technology have made it possible to reliably predict, diagnose, and suggest ways to remedy the low protein content and bioavailability of key micronutrients in food crops. Read More

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http://link.springer.com/10.1007/s10529-019-02655-9
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http://dx.doi.org/10.1007/s10529-019-02655-9DOI Listing
February 2019
8 Reads

Plasma nitriding under low temperature improves the endothelial cell biocompatibility of 316L stainless steel.

Biotechnol Lett 2019 Feb 28. Epub 2019 Feb 28.

Departamento de Ciências Animais, Universidade Federal Rural do Semi-Árido, UFERSA, Av. Francisco Mota, 572 -Bairro Costa e Silva, Mossoró, RN, CEP: 59.625-900, Brazil.

Objectives: To evaluate the effects of the surface modification of 316L stainless steel (SS) by low-temperature plasma nitriding on endothelial cells for stent applications.

Results: X-ray diffraction (XRD) confirmed the incorporation of nitrogen into the treated steel. The surface treatment significantly increased SS roughness and hydrophilic characteristics. Read More

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http://dx.doi.org/10.1007/s10529-019-02657-7DOI Listing
February 2019
1.591 Impact Factor

Chromatographic purification of recombinant human erythropoietin.

Biotechnol Lett 2019 Feb 27. Epub 2019 Feb 27.

Department of Chemical and Biochemical Engineering, Faculty of Chemical and Food Technology, Institute of Chemical and Environmental Engineering, Slovak University of Technology, Radlinského 9, 812 37, Bratislava, Slovakia.

Recombinant human erythropoietin is a valuable therapeutic protein used in the treatment of several serious diseases. It exists in different isoforms and is produced by genetically modified mammalian cells such as Chinese hamster ovary or human embryonic kidney cells. As for other biopharmaceutical drugs, a key factor for its successful industrial production is to achieve a high degree of purity and to decrease the content of critical impurities to trace amounts. Read More

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http://link.springer.com/10.1007/s10529-019-02656-8
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http://dx.doi.org/10.1007/s10529-019-02656-8DOI Listing
February 2019
6 Reads

Bacteriocin encapsulation for food and pharmaceutical applications: advances in the past 20 years.

Biotechnol Lett 2019 Feb 9. Epub 2019 Feb 9.

Cuerpo Académico de Biotecnología Agroalimentaria, Instituto de Ciencias Agropecuarias, Universidad Autónoma del Estado de Hidalgo, Av. Universidad km 1, Rancho Universitario, C.P. 43600, Tulancingo de Bravo, Hidalgo, Mexico.

The encapsulation of bacteriocins from lactic acid bacteria has involved several methods to protect them from unfavourable environmental conditions and incompatibilities. This review encompasses different methods for the encapsulation of bacteriocins and their applications in both food and pharmaceutical fields. Based on the bibliometric analysis of publications from well-reputed journals including different available patents during the period from 1996 to 2017, 135 articles and 60 patents were collected. Read More

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http://dx.doi.org/10.1007/s10529-018-02635-5DOI Listing
February 2019
2 Reads

Preparation of high-quality sunflower seed protein with a new chlorogenic acid hydrolase from Aspergillus niger.

Biotechnol Lett 2019 Feb 8. Epub 2019 Feb 8.

State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, 214122, China.

Objective: To investigate the biochemical and enzymatic properties of chlorogenic acid hydrolase (ChlH) from Aspergillus niger SD14.721 and its applicability in sunflower seed protein processing.

Results: The ChlH with two identical subunits (97 kDa) was highly stable. Read More

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http://dx.doi.org/10.1007/s10529-019-02654-wDOI Listing
February 2019

Engineering the cytosolic NADH availability in lager yeast to improve the aroma profile of beer.

Biotechnol Lett 2019 Mar 1;41(3):363-369. Epub 2019 Feb 1.

Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, 214122, China.

Objective: To improve the aroma profile of beer by using metabolic engineering to increase the availability of cytosolic NADH in lager yeast.

Results: To alter NADH levels in lager yeast, the native FDH1 (YOR388C) encoding NAD-dependent formate dehydrogenase was overexpressed in the yeast strain M14, yielding strain M-FDH1. This led to a simultaneous increase of NADH availability and NADH/NAD ratio in the M-FDH1 strain during fermentation. Read More

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http://link.springer.com/10.1007/s10529-019-02653-x
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http://dx.doi.org/10.1007/s10529-019-02653-xDOI Listing
March 2019
6 Reads

Biosynthesis of silver nanoparticles by cell-free extracts from some bacteria species for dye removal from wastewater.

Biotechnol Lett 2019 Mar 28;41(3):379-389. Epub 2019 Jan 28.

Chemistry Department, Faculty of Science, Tanta University, Tanta, 31527, Egypt.

Objectives: To investigate the biosynthesis of silver nanoparticles (AgNPs) using extracts of some bacterial isolates Bacillus pumilus, Bacillus paralicheniformis and Sphingomonas paucimobilis. The formation of AgNPs was detected by the change in color into yellow and confirmed by the UV-Vis spectroscopy. The nanoparticles were characterized by X-ray diffraction (XRD), transmission electron microscopy (TEM) and Fourier transform infrared spectroscopy (FTIR). Read More

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http://dx.doi.org/10.1007/s10529-019-02652-yDOI Listing
March 2019
1 Read
1.591 Impact Factor

Desorption of heavy metals from metal loaded sorbents and e-wastes: A review.

Biotechnol Lett 2019 Mar 25;41(3):319-333. Epub 2019 Jan 25.

Microbial Biotechnology Laboratory, School of Biosciences and Technology, VIT University, Vellore, Tamil Nadu, 632014, India.

In recent era, with increasing heavy metal pollution, several sorbents are used to remove heavy metals from environment. Application of chemical adsorbents and biosorbents for uptake of heavy metals from soil and waste water is studied thoroughly. Very less attention has been paid to the recovery of heavy metals from sorbents and their reuse. Read More

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http://dx.doi.org/10.1007/s10529-019-02650-0DOI Listing
March 2019
1.591 Impact Factor

Current state-of-the-art in plant-based antibody production systems.

Biotechnol Lett 2019 Mar 25;41(3):335-346. Epub 2019 Jan 25.

Laboratory of Biotechnology, ENEA Casaccia Research Center, Via Anguillarese 301, 00123, Rome, Italy.

Monoclonal antibodies represent the major class of biopharmaceutical products (for therapeutics and diagnostics) with an increasing demand that reaches several tons per year worldwide. Traditional large-scale manufacturing processes are based on stirred tank bioreactors for the growth of Chinese Hamster Ovary cells (CHO) which requires high initial investments and production costs. Therefore, there is an urgent need for alternative production platforms that can at least act as a complement to the over-exploited mammalian fermentation systems. Read More

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http://dx.doi.org/10.1007/s10529-019-02651-zDOI Listing
March 2019
1 Read

Pinpointing the L-phenylalanine binding sites of TyrR using biosensors and computer-aided simulation.

Biotechnol Lett 2019 Mar 24;41(3):401-408. Epub 2019 Jan 24.

Tianjin Institutes of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, People's Republic of China.

Objectives: To determine the binding sites for L-phenylalanine in TyrR protein via a rational mutation analysis combining biosensors and computer-aided simulation.

Results: TyrR protein of Escherichia coli is the chief transcriptional regulator of several genes essential for the biosynthesis and transport of aromatic amino acids. The identification of ligand-binding sites is often the starting point for protein function annotation and structure-based protein design. Read More

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http://dx.doi.org/10.1007/s10529-019-02645-xDOI Listing
March 2019
1 Read
1.591 Impact Factor

Mesenchymal stem cell sheets: a new cell-based strategy for bone repair and regeneration.

Biotechnol Lett 2019 Mar 24;41(3):305-318. Epub 2019 Jan 24.

Department of Orthopaedics, Shaoxing People's Hospital, Shaoxing Hospital, Zhejiang University School of Medicine, 568# Zhongxing North Road, Shaoxing, 312000, Zhejiang, People's Republic of China.

Mesenchymal stem cells (MSCs), a class of adult stem cells, are considered a promising source for bone regeneration. Although combining MSCs with biomaterial scaffolds offers an interesting clinical strategy for bone tissue engineering, the presence of the scaffolds could induce an undesirable effect on cell-cell interactions. Moreover, before the application of scaffold materials in bone tissue reconstruction, cells must be manipulated with proteolytic enzymes, such as trypsin or dispase that degrade extracellular matrix (ECM) molecules and cell surface proteins, which can result in the cell damage and loss of cellular activity. Read More

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http://link.springer.com/10.1007/s10529-019-02649-7
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http://dx.doi.org/10.1007/s10529-019-02649-7DOI Listing
March 2019
5 Reads

Production of recombinant human factor IX by propeptide modification in Drosophila S2 cell line.

Biotechnol Lett 2019 Mar 23;41(3):347-355. Epub 2019 Jan 23.

Department of Molecular Medicine, Institute of Medical Biotechnology, National Institute of Genetic Engineering and Biotechnology, P.O. Box: 14965/161, Tehran, Iran.

Objective: To compare the effect of pre-propeptide (pre-pro) of the human prothrombin (hPT), with both the native and an R-9N mutant forms of the human factor IX (hFIX) pre-pro on the hFIX carboxylation, in Drosophila cell.

Results: The three different pre-pro sequences, equipped with Drosophila Kozak, were joined to the mature hFIX cDNA and were subjected to transient expression analysis of hFIX in the S2 Drosophila cells, compared to that of a native hFIX cDNA, with its native Kozak. Replacement of the hFIX pre-pro sequence with that of hPT increased the biological activity of hFIX, significantly. Read More

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http://dx.doi.org/10.1007/s10529-019-02643-zDOI Listing
March 2019
1 Read

Cloning and characterization of α-L-rhamnosidase from Chloroflexus aurantiacus and its application in the production of isoquercitrin from rutin.

Biotechnol Lett 2019 Mar 21;41(3):419-426. Epub 2019 Jan 21.

Department of Food Science and Technology, Catholic University of Daegu, Hayang, South Korea.

Objective: This study was conducted to characterize recombinant α-L-rhamnosidase from Chloroflexus aurantiacus and apply the enzyme in the production of isoquercitrin from rutin.

Results: The α-L-rhamnosidase from C. aurantiacus was cloned and expressed in Escherichia coli BL21 and purified as a soluble enzyme. Read More

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http://dx.doi.org/10.1007/s10529-019-02648-8DOI Listing

Isoflavone production in hairy root cultures and plantlets of Trifolium pratense.

Biotechnol Lett 2019 Mar 19;41(3):427-442. Epub 2019 Jan 19.

Laboratory of Pharmacognosy, Department of Raw Material Production, Federal University of Rio Grande do Sul, Porto Alegre - UFRGS, Porto Alegre, 90610-000, Brazil.

Objectives: The aim of this study was to develop a Trifolium pratense hairy root (HR) production protocol and select HR lines with high isoflavone yield following elicitor treatments.

Results: We obtained 13 independent HR lines, producing approximately three times more isoflavonoids than seedlings (3.3 mg/g dry weight) and in which 27 isoflavonoids were detected. Read More

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http://dx.doi.org/10.1007/s10529-018-02640-8DOI Listing

High-throughput amplicon sequencing demonstrates extensive diversity of xylanase genes in the sediment of soda lake Dabusu.

Biotechnol Lett 2019 Mar 14;41(3):409-418. Epub 2019 Jan 14.

College of Biological Science and Engineering, Fuzhou University, Qi Shan Campus, No. 2 Xueyuan Road, University Town, Fuzhou, 350116, Fujian, People's Republic of China.

Objective: To explore the diversity of glycoside hydrolase family 10 xylanase genes in the sediment of soda lake Dabusu by using high-throughput amplicon sequencing based on the Illumina HiSeq2500 platform.

Results: A total of 227,420 clean reads, representing approximately 49.5 M bp, were obtained. Read More

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http://link.springer.com/10.1007/s10529-019-02646-w
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http://dx.doi.org/10.1007/s10529-019-02646-wDOI Listing
March 2019
4 Reads

Cloning and characterization of a Flavin-free oxygen-insensitive azoreductase from Klebsiella oxytoca GS-4-08.

Authors:
Jing-Qiu Hua Lei Yu

Biotechnol Lett 2019 Mar 11;41(3):371-378. Epub 2019 Jan 11.

Department of Environmental Engineering, Nanjing Forestry University, Nanjing, 210037, China.

Objectives: This study reports the identification of a new bacterial azoreductase (AzoR) from Klebsiella oxytoca GS-4-08, its heterologous production in Escherichia coli and the decolorization of azo dyes.

Results: The AzoR, as a flavin-free, oxygen-insensitive enzyme, has a molecular mass of 22 kDa and a high substrate specificity for methyl red (MR). In the presence of 5 mM of NADH, the enzyme activity for decolorization of 50 mg l of MR reached 5. Read More

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http://dx.doi.org/10.1007/s10529-019-02647-9DOI Listing
March 2019
1 Read

Enhanced degradation of hydrocarbons by gamma ray induced mutant strain of Pseudomonas putida.

Biotechnol Lett 2019 Mar 4;41(3):391-399. Epub 2019 Jan 4.

Soil and Environmental Biotechnology Division, National Institute for Biotechnology and Genetic Engineering (NIBGE), P.O. Box 577, Jhang Road, Faisalabad, Pakistan.

Soil contamination due to petroleum hydrocarbons is a ubiquitous environmental problem for which efficient remediation alternatives are required. Application of hydrocarbons degrading bacteria with enhanced degradation potential is such an alternative. The aim of present investigation was to induce mutagenicity in Pseudomonas putida through gamma-ray irradiation for the enhanced degradation of crude oil. Read More

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http://link.springer.com/10.1007/s10529-019-02644-y
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http://dx.doi.org/10.1007/s10529-019-02644-yDOI Listing
March 2019
10 Reads

Construction of DNA-NanoLuc luciferase conjugates for DNA aptamer-based sandwich assay using Rep protein.

Biotechnol Lett 2019 Mar 3;41(3):357-362. Epub 2019 Jan 3.

Department of Life Science and Technology, School of Life Science and Technology, Tokyo Institute of Technology, 4259 Nagatsuta, Midori-Ku, Yokohama, 226-8502, Japan.

Objective: We developed a DNA-NanoLuc luciferase (NnaoLuc) conjugates for DNA aptamer-based sandwich assay using the catalytic domain of the replication initiator protein derived from porcine circovirus type 2 (pRep).

Results: For construction of DNA aptamer and NanoLuc conjugate using the catalytic domain of Rep from PCV2. pRep fused to NanoLuc was genetically constructed and expressed in E. Read More

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http://link.springer.com/10.1007/s10529-018-02641-7
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http://dx.doi.org/10.1007/s10529-018-02641-7DOI Listing
March 2019
3 Reads

Reactivation of rolB transgene expression in Vitis amurensis Rupr. cells upon retransformation with 2b gene from Cucumovirus isolate NK.

Biotechnol Lett 2019 Mar 2;41(3):443-451. Epub 2019 Jan 2.

Laboratory of Biotechnology, Federal Scientific Center of East Asia Terrestrial Biodiversity, Far Eastern Branch of Russian Academy of Sciences, Vladivostok, Russia, 690022.

Objective: Studies concerning 2b protein from Cucumovirus showed 2b to effectively repress functioning of the plant silencing complex, current study aimed whether retransformation with 2b gene able to restore silenced transgene expression in plant cells.

Results: A rolB-transgenic cell culture of Vitis amurensis Rupr. that was continuously subcultured during more than 10 years and exhibited decreased transcription of the rolB transgene was retransformed with the 2b gene of Cucumovirus-NK. Read More

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http://dx.doi.org/10.1007/s10529-018-02642-6DOI Listing
March 2019
1 Read

Thermophilin 109 is a naturally produced broad spectrum bacteriocin encoded within the blp gene cluster of Streptococcus thermophilus.

Biotechnol Lett 2019 Feb 18;41(2):283-292. Epub 2018 Dec 18.

Dairy and Functional Foods Research Unit, Agricultural Research Service, USDA, 600 E. Mermaid Lane, Wyndmoor, PA, 19038, USA.

Objectives: To demonstrate that S. thermophilus ST109 produces an antimicrobial peptide encoded within the bacteriocin-like peptide (blp) gene cluster, and to determine its broad spectrum activity against potential human pathogens.

Results: Analysis of the cell free supernatant (CFS) revealed that antimicrobial activity was associated with the presence of a heat-stable peptide of approximately 5-6 kDa; and activity was lost after protease treatment or exposure to α-amylase. Read More

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http://dx.doi.org/10.1007/s10529-018-02637-3DOI Listing
February 2019
1 Read

Chemical transformation mediated CRISPR/Cas9 genome editing in Escherichia coli.

Biotechnol Lett 2019 Feb 13;41(2):293-303. Epub 2018 Dec 13.

College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, 310014, Zhejiang, People's Republic of China.

Objectives: To develop a convenient chemical transformation mediated CRISPR/Cas9 (CT-CRISPR/Cas9) system for genome editing in Escherichia coli.

Results: Here, we have constructed a CT-CRISPR/Cas9 system, which can precisely edit bacterial genome (replacing, deleting, inserting or point mutating a target gene) through chemical transformation. Compared with the traditional electroporation mediated CRISPR/Cas9 (ET-CRISPR/Cas9) system, genome editing with the CT-CRISPR/Cas9 system is much cheaper and simpler. Read More

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http://dx.doi.org/10.1007/s10529-018-02639-1DOI Listing
February 2019
1 Read

The marine Gram-negative bacterium Novosphingobium sp. PP1Y as a potential source of novel metabolites with antioxidant activity.

Biotechnol Lett 2019 Feb 12;41(2):273-281. Epub 2018 Dec 12.

Department of Chemical Sciences, University Federico II of Naples, Via Cinthia 26, 80127, Naples, Italy.

Objective: The antioxidant activity and protective effect of a methanolic extract obtained from the marine Gram-negative bacterium Novosphingobium sp. PP1Y, isolated from the surface water of a polluted area in the harbour of Pozzuoli (Naples, Italy), was evaluated.

Results: The extract was tested in vitro on epithelial colorectal adenocarcinoma cells and in vivo on Caenorhabditis elegans. Read More

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http://link.springer.com/10.1007/s10529-018-02636-4
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http://dx.doi.org/10.1007/s10529-018-02636-4DOI Listing
February 2019
5 Reads

Progress in antibiotic susceptibility tests: a comparative review with special emphasis on microfluidic methods.

Biotechnol Lett 2019 Feb 12;41(2):221-230. Epub 2018 Dec 12.

School of Mechanical Engineering, Korea University of Technology and Education, Cheonan, Chungnam, 31253, South Korea.

Antibiotic susceptibility test (AST) is an umbrella term for techniques to determine the susceptibility of bacteria to antibiotics. The antibiotic-resistant bacteria are a major threat to public health and a directed therapy based on accurate AST results is paramount in resistance control. Here we have briefly covered the progress of conventional, molecular, and automated AST tools and their limitations. Read More

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http://dx.doi.org/10.1007/s10529-018-02638-2DOI Listing
February 2019

Oligosaccharides as co-encapsulating agents: effect on oral Lactobacillus fermentum survival in a simulated gastrointestinal tract.

Biotechnol Lett 2019 Feb 10;41(2):263-272. Epub 2018 Dec 10.

School of Food Science and Technology, Jiangnan University, Jiangsu, People's Republic of China.

Objectives: Four kinds of oligosaccharides were used as co-encapsulating agents to test the effect of extrusion-based microencapsulation on protection of Lactobacillus fermentum L7 against exposure to simulated gastric and intestinal juices as well as long-term refrigeration storage at 4 °C.

Results: The combination of alginate with galacto-oligosaccharides, isomalto-oligosaccharides, fructo-oligosaccharides, and xylo-oligosaccharides, or alginate alone exhibited good properties of the beads. The diameters of the cell beads co-encapsulated with oligosaccharides and encapsulated with alginate alone were similar, in the range of 2. Read More

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http://link.springer.com/10.1007/s10529-018-02634-6
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http://dx.doi.org/10.1007/s10529-018-02634-6DOI Listing
February 2019
2 Reads

Solvent stable microbial lipases: current understanding and biotechnological applications.

Biotechnol Lett 2019 Feb 7;41(2):203-220. Epub 2018 Dec 7.

Dublin Institute of Technology, Dublin 1, Ireland.

Objective: This review examines on our current understanding of microbial lipase solvent tolerance, with a specific focus on the molecular strategies employed to improve lipase stability in a non-aqueous environment.

Results: It provides an overview of known solvent tolerant lipases and of approaches to improving solvent stability such as; enhancing stabilising interactions, modification of residue flexibility and surface charge alteration. It shows that judicious selection of lipase source supplemented by appropriate enzyme stabilisation, can lead to a wide application spectrum for lipases. Read More

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http://dx.doi.org/10.1007/s10529-018-02633-7DOI Listing
February 2019

Comparative features between recombinant lipases CALA-like from U. maydis and CALA from C. antarctica in thermal stability and selectivity.

Biotechnol Lett 2019 Feb 5;41(2):241-252. Epub 2018 Dec 5.

Biotecnología Industrial, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco A.C, Camino el arenero 1227, El Bajío del arenal, 45019, Zapopan, Jalisco, Mexico.

Objectives: Ustilago maydis lipase A (UMLA) expressed in Pichia pastoris was compared with Candida antarctica lipase A (CALA) to study its biochemical properties such as thermostability and selectivity.

Results: UMLA had similar behavior to its homologue CALA regarding the effect of pH and temperature on enzymatic activity, substrate preference and selectivity. Both lipases were active on insoluble triglycerides as well as natural oils and hydrolyzed preferably esters with short and medium acyl and alkyl chains. Read More

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http://link.springer.com/10.1007/s10529-018-2630-4
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http://dx.doi.org/10.1007/s10529-018-2630-4DOI Listing
February 2019
5 Reads

Biogas from microalgae: an overview emphasizing pretreatment methods and their energy return on investment (EROI).

Biotechnol Lett 2019 Feb 1;41(2):193-201. Epub 2018 Dec 1.

School of Chemistry, Federal University of Rio de Janeiro, Av. Athos da Silveira Ramos, no 149, Bloco E, Sala 200, Cidade Universitária, Rio de Janeiro, 21941-909, Brazil.

Studies have reported enhancements in methane yield from pretreatment methods that benefit the anaerobic digestion (AD) of microalgae. However, energy return on investment (EROI), i.e. Read More

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http://dx.doi.org/10.1007/s10529-018-2629-xDOI Listing
February 2019

Fumaric acid: production and separation.

Biotechnol Lett 2019 Jan 1;41(1):47-57. Epub 2018 Dec 1.

Faculty of Chemical Engineering and Environmental Protection "Cristofor Simionescu", "Gheorghe Asachi" Technical University of Iaşi, Iasi, Romania.

Fumaric acid is a valuable compound used in foods, beverages, detergents, animal feed, pharmaceuticals and miscellaneous industrial products. It is produced on a large scale by the petrochemical route but the current tendency is towards implementing "green production" and environmental friendly technologies like biotechnological production of fumaric acid using low-cost raw materials. In this context, numerous studies focus on improving the fermentation process not only by using renewable raw material and genetically modified microorganisms, but also by developing and applying different downstream techniques for easy recovery of fumaric acid from the fermented broth. Read More

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http://link.springer.com/10.1007/s10529-018-2628-y
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http://dx.doi.org/10.1007/s10529-018-2628-yDOI Listing
January 2019
1 Read

Improved yield of rhEPO in CHO cells with synthetic 5' UTR.

Biotechnol Lett 2019 Feb 30;41(2):231-239. Epub 2018 Nov 30.

National Institute for Cellular Biotechnology, Dublin City University, Glasnevin, Dublin, D09 NR58, Ireland.

The impact of local structure on mRNA translation is not well-defined pertaining to the 5' UTR. Reports suggest structural remodelling of the 5' UTR can significantly influence mRNA translation both in cis and trans however a new layer of complexity has been applied to this model with the now known reversible post-transcriptional chemical modification of RNA. N-methyladenosine (mA) is the most abundant internal base modification in mammalian mRNA. Read More

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http://dx.doi.org/10.1007/s10529-018-2632-2DOI Listing
February 2019
6 Reads

Green asymmetric reduction of acetophenone derivatives: Saccharomyces cerevisiae and aqueous natural deep eutectic solvent.

Biotechnol Lett 2019 Feb 3;41(2):253-262. Epub 2018 Dec 3.

Faculty of Food Technology and Biotechnology, University of Zagreb, Pierotijeva 6, 10000, Zagreb, Croatia.

Objective: Chiral building blocks [(S)-1-(3-methylphenyl)ethanol, (S)-1-(3,4-dimethylphenyl)ethanol and (S)-1-(2,4,6-trimethylphenyl)ethanol] for drug synthesis were prepared using two green approaches: (1) the yeast Saccharomyces cerevisiae as the biocatalyst and (2) the natural deep eutectic solvents (NADES) as the alternative solvents. Three different NADES with different water contents were prepared and screened for the highest conversion and enantiomeric excess of reduction of 1-(3-methylphenyl)ethanone, 1-(3,4-dimethylphenyl)ethanone (DMPA) and 1-(2,4,6-trimethyphenyl)ethanone by S. cerevisiae. Read More

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http://dx.doi.org/10.1007/s10529-018-2631-3DOI Listing
February 2019
2 Reads

Enhancing thermostability and removing hemin inhibition of Rhodopseudomonas palustris 5-aminolevulinic acid synthase by computer-aided rational design.

Biotechnol Lett 2019 Jan 29;41(1):181-191. Epub 2018 Nov 29.

Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, China.

Objective: To enhance the thermostability and deregulate the hemin inhibition of 5-aminolevulinic acid (ALA) synthase from Rhodopseudomonas palustris (RP-ALAS) by a computer-aided rational design strategy.

Results: Eighteen RP-ALAS single variants were rationally designed and screened by measuring their residual activities upon heating. Among them, H29R and H15K exhibited a 2. Read More

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http://dx.doi.org/10.1007/s10529-018-2627-zDOI Listing
January 2019
2 Reads

Enzyme-catalyzed regio-selective demethylation of papaverine by CYP105D1.

Biotechnol Lett 2019 Jan 21;41(1):171-180. Epub 2018 Nov 21.

Jiangsu Key Laboratory of TCM Evaluation and Translational Research, School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing, 211198, Jiangsu, China.

Objectives: To investigate the regio-selective demethylation of papaverine by CYP105D1 and develop a whole-cell biocatalytic system for the preparative synthesis of 6-O-demethyl-papaverine.

Results: CYP105D1 from Streptomyces griseus ATCC 13273 was used for the regioselective demethylation of papaverine at C-6 using putidaredoxin reductase (PDR) and putidaredoxin (Pdx) as the electron transport system. The Km value of CYP105D1 towards papaverine was estimated to be 92. Read More

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http://dx.doi.org/10.1007/s10529-018-2626-0DOI Listing
January 2019