2,523 results match your criteria Biotechnology Journal[Journal]


Bioinspired Amorphous Nanoparticles of Polyphosphate and Ca-Carbonate Improve the Self-Healing Property of Cements.

Biotechnol J 2020 Jun 4:e2000101. Epub 2020 Jun 4.

ERC Advanced Investigator Grant Research Group at the Institute for Physiological Chemistry, University Medical Center of the Johannes Gutenberg University, Duesbergweg 6, Mainz, 55128, Germany.

Cement is used both as a construction material and for medical applications. Previously it has been shown that the physiological polymer inorganic polyphosphate (polyP) is morphogenetically active in regeneration of skin, bone and cartilage. The present study investigates the question if this polymer is also a suitable additive to improve the self-healing capacity not only of construction cement but also of inorganic bone void fillers. Read More

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http://dx.doi.org/10.1002/biot.202000101DOI Listing

Production of Substituted Styrene Bioproducts from Lignin and Lignocellulose Using Engineered Pseudomonas Putida KT2440.

Biotechnol J 2020 Jun 2:e1900571. Epub 2020 Jun 2.

Department of Chemistry, University of Warwick, Coventry, CV4 7AL, U.K.

Ferulic acid is a renewable chemical found in lignocellulose from grasses such as wheat straw and sugarcane. Pseudomonas putida is able to liberate and metabolise ferulic acid from plant biomass. Deletion of the hydroxycinnamoyl-CoA hydratase-lyase gene (ech) produced a strain of P. Read More

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http://dx.doi.org/10.1002/biot.201900571DOI Listing

Inorganic Porous Nanoparticles for Drug Delivery in Antitumoral Therapy.

Biotechnol J 2020 Jun 1:e2000150. Epub 2020 Jun 1.

Dpto. Materiales y Producción Aeroespacial, ETSI Aeronáutica y del Espacio, Universidad Politécnica de Madrid, Madrid, 28040, Spain.

Background: The use of nanoparticles in oncology to deliver chemotherapeutic agents has received considerable attention in the last decades due to their tendency to be passively accumulated in solid tumors. Besides this remarkable property, the surface of these nanocarriers can be decorated with targeting moieties capable to recognize malignant cells which lead to selective nanoparticle uptake mainly in the diseased cells, without affecting the healthy ones.

Purpose And Scope: Among the different nanocarriers which have been developed with this purpose, inorganic porous nanomaterials constitute some of the most interesting due to their unique properties such as excellent cargo capacity, high biocompatibility and chemical, thermal and mechanical robustness, among others. Read More

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http://dx.doi.org/10.1002/biot.202000150DOI Listing

Epitopes of Naturally Acquired and Vaccine-induced Anti-Ebola Virus Glycoprotein Antibodies in Single Amino Acid Resolution.

Biotechnol J 2020 May 28:e2000069. Epub 2020 May 28.

Max Planck Institute of Colloids and Interfaces, Department of Biomolecular Systems, Am Muehlenberg 1, Potsdam, 14476, Germany.

The Ebola virus (EBOV) can cause severe infections in humans, leading to a fatal outcome in a high percentage of cases. Neutralizing antibodies against the EBOV surface glycoprotein (GP) can prevent infections, demonstrating a straightforward way for an efficient vaccination strategy. Meanwhile, many different anti-EBOV antibodies have been identified, whereas the exact binding epitopes are often unknown. Read More

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http://dx.doi.org/10.1002/biot.202000069DOI Listing

The Effect of Container Surface Passivation on Aggregation of Intravenous Immunoglobulin Induced by Mechanical Shock.

Biotechnol J 2020 May 21:e2000096. Epub 2020 May 21.

Department of Chemical and Biological Engineering, University of Colorado Boulder, Boulder, Colorado, 80303, United States.

Aggregation of therapeutic proteins can result from a number of stress conditions encountered during their manufacture, transportation, and storage. This work shows the effects of two interrelated sources of protein aggregation: the chemistry and structure of the surface of the container in which the protein is stored, and mechanical shocks that may result from handling of the formulation. We investigated how different mechanical stress conditions (dropping, tumbling and agitation) and container surface passivation affected the stability of solutions of intravenous immunoglobulin (IVIG). Read More

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http://dx.doi.org/10.1002/biot.202000096DOI Listing

Molecular Targets for the Testing of COVID-19.

Biotechnol J 2020 May 18:e2000152. Epub 2020 May 18.

Department of Biological Science and Technology, National Chiao Tung University, Hsinchu, 300, Taiwan.

The pandemic outbreaks of coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), spread all over the world in a short period of time. Efficient identification of the infection by SARS-CoV-2 has been one of the most important tasks to facilitate all the following counter measurements in dealing with the infectious disease. In Taiwan, a COVID-19 Open Science Platform adheres to the spirit of open science: sharing sources, data, and methods to promote progress in academic research while corroborating findings from various disciplines has established in mid-February 2020, for collaborative research in support of the development of detection methods, therapeutics, and a vaccine for COVID-19. Read More

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http://dx.doi.org/10.1002/biot.202000152DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7267081PMC

Photosynthetic Reduction of Xylose to Xylitol Using Cyanobacteria.

Biotechnol J 2020 May 10:e1900354. Epub 2020 May 10.

Department of Chemical Engineering, National Tsing Hua University, 101, Section 2, Kuang-Fu Road, Hsinchu, ROC 30013, Taiwan.

Photosynthetic generation of reducing power makes cyanobacteria an attractive host for biochemical reduction compared to cell-free and heterotrophic systems, which require burning of additional resources for the supply of reducing equivalent. Here, using xylitol synthesis as an example, efficient uptake and reduction of xylose photoautotrophically in Synechococcus elongatus PCC7942 are demonstrated upon introduction of an effective xylose transporter from Escherichia coli (Ec-XylE) and the NADPH-dependent xylose reductase from Candida boidinii (Cb-XR). Simultaneous activation of xylose uptake and matching of cofactor specificity enabled an average xylitol yield of 0. Read More

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http://dx.doi.org/10.1002/biot.201900354DOI Listing

Emerging Technologies for the Electrochemical Detection of Bacteria.

Biotechnol J 2020 May 10:e2000140. Epub 2020 May 10.

Department of Chemical Engineering, Polytechnique Montreal, Canada.

Infections are a huge economic liability to the health care system, although real time detection could allow early treatment protocols to avoid some of this cost and patient morbidity and mortality. Pseudomonas aeruginosa (PA) is a drug-resistant gram-negative bacterium found ubiquitously in clinical settings, accounting for up to 27% of hospital acquired infections. PA secretes a vast array of molecules, ranging from secondary metabolites to quorum sensing molecules, of which many can be exploited to monitor bacterial presence. Read More

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http://dx.doi.org/10.1002/biot.202000140DOI Listing

Transcription Factor ArcA is a Flux Sensor for the Oxygen Consumption Rate in Escherichia Coli.

Biotechnol J 2020 May 8:e1900353. Epub 2020 May 8.

Department of Bioinformatic Engineering, Graduate School of Information Science and Technology, Osaka University, 1-5 Yamadaoka, Suita, Osaka, 565-0871, Japan.

Oxygen supply is one of the major factors determining the metabolic state in microorganisms, and it affects the productivity of various compounds during bioproduction. In Escherichia coli, the expression levels of numerous metabolic genes are regulated by transcription factors in response to changes in environmental oxygen conditions. Even at a single concentration of dissolved oxygen, the amount of available oxygen can change depending on the oxygen transfer coefficient. Read More

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http://dx.doi.org/10.1002/biot.201900353DOI Listing

Fluorescent Biosensor of CDK5 Kinase Activity in Glioblastoma Cell Extracts and Living Cells.

Biotechnol J 2020 May 7:e1900474. Epub 2020 May 7.

Institut des Biomolécules Max Mousseron, CNRS, UMR 5247, Université de Montpellier, Faculté de Pharmacie, 15, Av. Charles Flahault, Montpellier, 34093, France.

CDK5 plays a major role in neuronal functions, and is hyperactivated in neurodegenerative pathologies as well as in glioblastoma and neuroblastoma. Although this kinase constitutes an established biomarker and pharmacological target, there are few means of probing its activity in cell extracts or in living cells. To this aim a fluorescent peptide reporter of CDK5 kinase activity, derived from a library of CDK5-specific substrates, is engineered and its ability to respond to recombinant CDK5/p25 is established and CDK5 activity in glioblastoma cell extracts is reported on through sensitive changes in fluorescence intensity. Read More

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http://dx.doi.org/10.1002/biot.201900474DOI Listing

Gas-Sensing Transcriptional Regulators.

Biotechnol J 2020 May 3:e1900345. Epub 2020 May 3.

Department of Chemical Engineering, Pohang University of Science and Technology, Pohang, 37673, Republic of Korea.

Gas molecules are ubiquitous in the environment and are used as nutrient and energy sources for living organisms. Many organisms, therefore, have developed gas-sensing systems to respond efficiently to changes in the atmospheric environment. In microorganisms and plants, two-component systems (TCSs) and transcription factors (TFs) are two primary mechanisms to sense gas molecules. Read More

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http://dx.doi.org/10.1002/biot.201900345DOI Listing

Bone-Inspired Tube Filling Decellularized Matrix of Toad Cartilage Provided an Osteoinductive Microenvironment for Mesenchymal Stem Cells to Facilitate the Radius Defect Repair of Rabbit.

Biotechnol J 2020 Apr 30:e2000004. Epub 2020 Apr 30.

Department of Pharmaceutics, School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou, Zhejiang Province, 325035, China.

Toad bone not only contains the rich cartilage-like matrix but also presents low immunogenicity. It is inferred that decellularized toad bone matrix (dBECM) may provide the more profitable osteoinductive microenvironment for mesenchymal stem cells (MSCs) to promote the repair of bone defects. Herein, a hollow bone-inspired tube is first made from hydroxyapatite (HA) and poly (γ-glutamic acid) (PGA), and then MSCs/dBECM hydrogel is uniformly filled to its central cavity, constructing a biomimetic bone (dBECM + MSCs - PGA + HA). Read More

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http://dx.doi.org/10.1002/biot.202000004DOI Listing

Micropipette Aspiration-Based Assessment of Single Channel Water Permeability.

Biotechnol J 2020 Apr 29:e1900450. Epub 2020 Apr 29.

Institute of Biophysics, Johannes Kepler University Linz, Linz, 4020, Austria.

Measurements of the unitary hydraulic conductivity of membrane channels, p , may be hampered by difficulties in producing sufficient quantities of purified and reconstituted proteins. Low yield expression, the purely empiric choice of detergents, as well as protein aggregation and misfolding during reconstitution may result in an average of less than one reconstituted channel per large unilamellar vesicle. This limits their applicability for p measurements, independent of whether light scattering or fluorescence quenching of encapsulated dyes is monitored. Read More

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http://dx.doi.org/10.1002/biot.201900450DOI Listing

Anything but Conventional Chromatography Approaches in Bioseparation.

Biotechnol J 2020 Apr 25:e1900274. Epub 2020 Apr 25.

Department of Biotechnology, Delft University of Technology, Van der Maasweg 9, Delft, 2629 HZ, The Netherlands.

While packed bed chromatography, known as conventional chromatography, has been serving the biopharmaceutical industry for decades as the bioseparation method of choice, alternative approaches are likely to take an increasing leading role in the next few years. The high number of new biological drugs under development, and the need to make biopharmaceuticals widely accessible, has been driving the academia and industry in the quest of anything but conventional chromatography approaches. In this perspective paper, these alternative approaches are discussed in view of current and future challenges in the downstream processing field. Read More

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http://dx.doi.org/10.1002/biot.201900274DOI Listing

Metal-Organic Framework Composites for Theragnostics and Drug Delivery Applications.

Biotechnol J 2020 Apr 24:e2000005. Epub 2020 Apr 24.

Adsorption & Advanced Materials Laboratory (A2ML), Department of Chemical Engineering & Biotechnology, University of Cambridge, Philippa Fawcett Drive, Cambridge, CB3 0AS, UK.

Among a plethora of nano-sized therapeutics, metal-organic frameworks (MOFs) have been some of the most investigated novel materials for, predominantly, cancer drug delivery applications. Due to their large drug uptake capacities and slow-release mechanisms, MOFs are desirable drug delivery vehicles that protect and transport sensitive drug molecules to target sites. The inclusion of other guest materials into MOFs to make MOF-composite materials has added further functionality, from externally triggered drug release to improved pharmacokinetics and diagnostic aids. Read More

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http://dx.doi.org/10.1002/biot.202000005DOI Listing

C Metabolic Flux Analysis of Escherichia coli Engineered for Gamma-Aminobutyrate Production.

Biotechnol J 2020 Apr 22:e1900346. Epub 2020 Apr 22.

Department of Chemical and Biological Engineering, Korea University, 145 Anam-ro, Seongbuk-gu, Seoul, 02841, Korea.

Escherichia coli is engineered for γ-aminobutyrate (GABA) production in glucose minimal medium. For this, overexpression of mutant glutamate decarboxylase (GadB) and mutant glutamate/GABA antiporter (GadC), as well as deletion of GABA transaminase (GabT), are accomplished. In addition, the carbon flux to the tricarboxylic acid cycle is engineered by the overexpression of gltA, ppc, or both. Read More

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http://dx.doi.org/10.1002/biot.201900346DOI Listing

Progress and Concept for COVID-19 Vaccine Development.

Authors:
Suh-Chin Wu

Biotechnol J 2020 Apr 18:e2000147. Epub 2020 Apr 18.

Department of Medical Science, Institute of Biotechnology, National Tsing Hua University, Hsinchu, 30013, Taiwan.

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http://dx.doi.org/10.1002/biot.202000147DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7235517PMC

Crystal Contact Engineering Enables Efficient Capture and Purification of an Oxidoreductase by Technical Crystallization.

Biotechnol J 2020 Apr 17:e2000010. Epub 2020 Apr 17.

Technische Universität München, Lehrstuhl für Bioverfahrenstechnik, Boltzmannstraße 15, Garching, 85748, Germany.

Technical crystallization is an attractive method to purify recombinant proteins. However, it is rarely applied due to the limited crystallizability of many proteins. To overcome this limitation, single amino acid exchanges are rationally introduced to enhance intermolecular interactions at the crystal contacts of the industrially relevant biocatalyst Lactobacillus brevis alcohol dehydrogenase (LbADH). Read More

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http://dx.doi.org/10.1002/biot.202000010DOI Listing

Modeling the Residence Time Distribution of Integrated Continuous Bioprocesses.

Biotechnol J 2020 Apr 17:e2000008. Epub 2020 Apr 17.

Austria Centre for Industrial Biotechnology, Muthgasse 11, Vienna, A-1190, Austria.

In response to the biopharmaceutical industry advancing from traditional batch operation to continuous operation, the Food and Drug Administration (FDA) has published a draft for continuous integrated biomanufacturing. This draft outlines the most important rules for establishing continuous integration. One of these rules is a thorough understanding of mass flows in the process. Read More

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http://dx.doi.org/10.1002/biot.202000008DOI Listing

Transporter Engineering for Microbial Manufacturing.

Biotechnol J 2020 Apr 16:e1900494. Epub 2020 Apr 16.

Department of Biochemical Engineering, Institute for Synthetic Biosystem, School of Chemistry and Chemical Engineering, Beijing Institute of Technology, Beijing, 100081, P. R. China.

Microbes play an important role in biotransformation and biosynthesis of biofuels, natural products, and polymers. Therefore, microbial manufacturing has been widely used in medicine, industry, and agriculture. However, common strategies including enzyme engineering, pathway optimization, and host engineering are generally inadequate to obtain an efficient microbial production system. Read More

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http://dx.doi.org/10.1002/biot.201900494DOI Listing

Oligonucleotides: Current Trends and Innovative Applications in the Synthesis, Characterization, and Purification.

Biotechnol J 2020 Apr 16:e1900226. Epub 2020 Apr 16.

Department of Chemistry, Materials and Chemical Engineering "Giulio Natta,", Politecnico di Milano, via Mancinelli 7, Milano, 20131, Italy.

Oligonucleotides (ONs) are gaining increasing importance as a promising novel class of biopharmaceuticals. Thanks to their fundamental role in gene regulation, they can be used to develop custom-made drugs (also called N-to-1) able to act on the gene expression at pre-translational level. With recent approvals of ON-based therapeutics by the Food and Drug Administration (FDA), a growing demand for high-quality chemically modified ONs is emerging and their market is expected to impressively prosper in the near future. Read More

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http://dx.doi.org/10.1002/biot.201900226DOI Listing

Chemical Production from Methanol Using Natural and Synthetic Methylotrophs.

Biotechnol J 2020 Apr 13:e1900356. Epub 2020 Apr 13.

Department of Biological Science and Technology, National Chiao Tung University, Hsinchu, 30010, Taiwan.

Methanol as a chemical feedstock is becoming increasingly important as it is derived from natural gas and is a feasible end-product for captured carbon dioxide. Biological conversion of methanol through natural and synthetic methylotrophs increases the chemical repertoire and is an important direction for one carbon (C1) based chemical economy. Advances in the metabolic engineering and synthetic biology enable development of microbial cell factories for converting methanol into various platform chemicals. Read More

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http://dx.doi.org/10.1002/biot.201900356DOI Listing

PASylation Enhances the Stability, Potency, and Plasma Half-Life of Interferon α-2a: A Molecular Dynamics Simulation.

Biotechnol J 2020 Apr 10:e1900385. Epub 2020 Apr 10.

Department of Aerospace Engineering, Sharif University of Technology, Azadi Ave. 11365-11155, Tehran, Iran.

In this study, the effectiveness of PASylation in enhancing the potency and plasma half-life of pharmaceutical proteins has been accredited as an alternative technique to the conventional methods such as PEGylation. Proline, alanine, and serine (PAS) chain has shown some advantages including biodegradability improvement and plasma half-life enhancement while lacking immunogenicity or toxicity. Although some experimental studies have been performed to find the mechanism behind PASylation, the detailed mechanism of PAS effects on the pharmaceutical proteins has remained obscure, especially at the molecular level. Read More

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http://dx.doi.org/10.1002/biot.201900385DOI Listing

Applications of Functional Metal-Organic Frameworks in Biosensors.

Biotechnol J 2020 Apr 9:e1900424. Epub 2020 Apr 9.

Institute of Medical Engineering, Department of Biophysics, School of Basic Medical Sciences, Health Science Center, Xi'an Jiaotong University, Xi'an, 710061, China.

In recent decades, fast advancements in the fields of metal-organic frameworks (MOFs) are providing unprecedented opportunities for the development of novel functional MOFs for various biosensing applications. Exciting progress is achieved due to the combination of MOFs with various functional components, which introduces novel structures and new features to the MOFs-based biosensing applications, such as higher stability, higher sensitivity, higher flexibility, and higher specificity. This review aims to be a comprehensive summary of the most recent advances in the development of functional MOFs for various biosensing applications, placing special attention on important contributions in recent 3 years. Read More

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http://dx.doi.org/10.1002/biot.201900424DOI Listing

Temperature Down-Shift Modifies Expression of UPR-/ERAD-Related Genes and Enhances Production of a Chimeric Fusion Protein in CHO Cells.

Biotechnol J 2020 Apr 9:e2000081. Epub 2020 Apr 9.

Manchester Institute of Biotechnology, Faculty of Science and Engineering, University of Manchester, Manchester, M1 7DN, UK.

Low culture temperature enhances the cell-specific productivity of Chinese hamster ovary (CHO) cells expressing varied recombinant (r-) proteins, but the mechanisms remain unclear. Regulation of unfolded protein response (UPR) pathway genes, such as transcriptional regulatory factors and endoplasmic reticulum (ER)-resident proteins, appear to be involved in the improvements of r-protein production under low temperature conditions. The transcriptional regulation of UPR-specific targets is studied in response to decreased culture temperature in relation to production of a difficult-to-express protein. Read More

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http://dx.doi.org/10.1002/biot.202000081DOI Listing

Biotechnological Production of Flavonoids: An Update on Plant Metabolic Engineering, Microbial Host Selection, and Genetically Encoded Biosensors.

Biotechnol J 2020 Apr 8:e1900432. Epub 2020 Apr 8.

Department of Chemical, Biochemical, and Environmental Engineering, University of Maryland, Baltimore, MD, 21250, USA.

Flavonoids represent a diversified family of phenylpropanoid-derived plant secondary metabolites. They are widely found in fruits, vegetables, and medicinal herbs. There has been increasing interest on flavonoids because of their proven bioactivity associated with anti-obesity and anti-cancer, anti-inflammatory and anti-diabetic activity. Read More

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http://dx.doi.org/10.1002/biot.201900432DOI Listing

Atomic Force Microscopy in Mechanoimmunology Analysis: A New Perspective for Cancer Immunotherapy.

Biotechnol J 2020 Apr 2:e1900559. Epub 2020 Apr 2.

Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, State and Local Joint Engineering Laboratory for Vascular Implants, College of Bioengineering, Chongqing, 400030, China.

Immunotherapy has remarkable success outcomes against hematological malignancies with high rates of complete remission. To date, many studies have been conducted to increase its effectiveness in other types of cancer. However, it still yields unsatisfying results in solid tumor therapy. Read More

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http://dx.doi.org/10.1002/biot.201900559DOI Listing

Overexpression of Transcription Factor ZNF1 of Glycolysis Improves Bioethanol Productivity under High Glucose Concentration and Enhances Acetic Acid Tolerance of Saccharomyces cerevisiae.

Biotechnol J 2020 Mar 20:e1900492. Epub 2020 Mar 20.

Division of Biochemical Technology, School of Bioresources and Technology, King Mongkut's University of Technology Thonburi, Bangkok, 10150, Thailand.

Saccharomyces cerevisiae offers an attractive platform for synthesis of biofuels and biochemical; however, robust strains that can withstand high substrate concentration and fermentation conditions are required. To improve the yield and productivity of bioethanol, modification of glucose metabolism and cellular stress adaptation is investigated. Specifically, the role of Znf1 transcription factor in metabolic regulation of glucose is characterized. Read More

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http://dx.doi.org/10.1002/biot.201900492DOI Listing

Biotransformation of 5-Hydroxymethylfurfural to 2,5-Furandicarboxylic Acid by a Syntrophic Consortium of Engineered Synechococcus elongatus and Pseudomonas putida.

Biotechnol J 2020 Mar 17:e1900357. Epub 2020 Mar 17.

Department of Chemical Engineering, National Taiwan University of Science and Technology, Taipei, 10607, Taiwan.

2,5-furandicarboxylic acid (FDCA) is one of the top platform chemicals that can be produced from biomass feedstock. To make the cost of industrial FDCA production compatible with plastics made from fossils, the price of substrates and process complexity should be reduced. The aim of this research is to create a CO -driven syntrophic consortium for the catalytic conversion of renewable biomass-derived 5-hydroxymethylfurfural (HMF) to FDCA. Read More

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http://dx.doi.org/10.1002/biot.201900357DOI Listing

Biotherapeutic Approaches in Atopic Dermatitis.

Biotechnol J 2020 Mar 16:e1900322. Epub 2020 Mar 16.

Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, 119228, Singapore.

The skin microbiome plays a central role in inflammatory skin disorders such as atopic dermatitis (AD). In AD patients, an imbalance between pathogenic Staphylococcus aureus (S. aureus) and resident skin symbionts creates a state of dysbiosis which induces immune dysregulation and impairs skin barrier function. Read More

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http://dx.doi.org/10.1002/biot.201900322DOI Listing
March 2020
3.708 Impact Factor

A Cyclin D1-Specific Single-Chain Variable Fragment Antibody that Inhibits HepG2 Cell Growth and Proliferation.

Biotechnol J 2020 Mar 14:e1900430. Epub 2020 Mar 14.

Key Laboratory for Molecular Enzymology and Engineering of the Ministry of Education, School of Life Sciences, Jilin University, Changchun, 130012, China.

Cyclin D1 is a key regulatory factor of the G1 to S transition during cell cycle progression. Aberrant cyclin D gene amplification and abnormal protein expression have been linked to hepatocellular carcinoma (HCC) tumorigenesis. Intrabodies, effective anticancer therapies that specifically inhibit target protein function within all intracellular compartments, may block cyclin D1 function. Read More

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http://dx.doi.org/10.1002/biot.201900430DOI Listing
March 2020
3.708 Impact Factor

Multi-Omics Reveals Impact of Cysteine Feed Concentration and Resulting Redox Imbalance on Cellular Energy Metabolism and Specific Productivity in CHO Cell Bioprocessing.

Biotechnol J 2020 Mar 13:e1900565. Epub 2020 Mar 13.

Department of Chemistry and Chemical Biology, Barnett Institute of Chemical and Biological Analysis, Northeastern University, Boston, MA, 02115, USA.

Chinese hamster ovary (CHO) cells are currently the primary host cell lines used in biotherapeutic manufacturing of monoclonal antibodies (mAbs) and other biopharmaceuticals. Cellular energy metabolism and endoplasmic reticulum (ER) stress are known to greatly impact cell growth, viability, and specific productivity of a biotherapeutic; but the molecular mechanisms are not fully understood. The authors previously employed multi-omics profiling to investigate the impact of a reduction in cysteine (Cys) feed concentration in a fed-batch process and found that disruption of the redox balance led to a substantial decline in cell viability and titer. Read More

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http://dx.doi.org/10.1002/biot.201900565DOI Listing

Microbial Engineering for Production of N-Functionalized Amino Acids and Amines.

Biotechnol J 2020 Mar 13:e1900451. Epub 2020 Mar 13.

Genetics of Prokaryotes, Biology and CeBiTec, Bielefeld University, Bielefeld, 33615, Germany.

N-functionalized amines play important roles in nature and occur, for example, in the antibiotic vancomycin, the immunosuppressant cyclosporine, the cytostatic actinomycin, the siderophore aerobactin, the cyanogenic glucoside linamarin, and the polyamine spermidine. In the pharmaceutical and fine-chemical industries N-functionalized amines are used as building blocks for the preparation of bioactive molecules. Processes based on fermentation and on enzyme catalysis have been developed to provide sustainable manufacturing routes to N-alkylated, N-hydroxylated, N-acylated, or other N-functionalized amines including polyamines. Read More

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http://dx.doi.org/10.1002/biot.201900451DOI Listing
March 2020
3.708 Impact Factor

Recent Advances in Sustainable Plastic Upcycling and Biopolymers.

Biotechnol J 2020 Mar 12:e1900489. Epub 2020 Mar 12.

Division of Chemical Engineering and Materials Science, Ewha Womans University, 52 Ewhayeodae-gil, Seodaemun-gu, Seoul, 03760, Republic of Korea.

Advances in scientific technology in the early twentieth century have facilitated the development of synthetic plastics that are lightweight, rigid, and can be easily molded into a desirable shape without changing their material properties. Thus, plastics become ubiquitous and indispensable materials that are used in various manufacturing sectors, including clothing, automotive, medical, and electronic industries. However, strong physical durability and chemical stability of synthetic plastics, most of which are produced from fossil fuels, hinder their complete degradation when they are improperly discarded after use. Read More

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http://dx.doi.org/10.1002/biot.201900489DOI Listing

Crosstalk between Endo/Exocytosis and Autophagy in Health and Disease.

Biotechnol J 2020 Mar 6:e1900267. Epub 2020 Mar 6.

Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology-Hellas, Heraklion, 70013, Greece.

Imbalance between the main intracellular degradative, trafficking and intercellular shuttling pathways has been implicated in disease pathogenesis. Autophagy controls degradation of cellular components, while vesicular trafficking permits transport of material in and out of the cell. Emerging evidence has uncovered the extensive interconnectivity between these pathways, which is crucial to maintain organismal homeostasis. Read More

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http://dx.doi.org/10.1002/biot.201900267DOI Listing
March 2020
3.708 Impact Factor

Nanobiohybrid Material-Based Bioelectronic Devices.

Biotechnol J 2020 Mar 6:e1900347. Epub 2020 Mar 6.

Department of Chemical and Biomolecular Engineering, Sogang University, 35 Baekbeom-Ro, Mapo-Gu, Seoul, 04107, Republic of Korea.

Biomolecules, especially proteins and nucleic acids, have been widely studied to develop biochips for various applications in scientific fields ranging from bioelectronics to stem cell research. However, restrictions exist due to the inherent characteristics of biomolecules, such as instability and the constraint of granting the functionality to the biochip. Introduction of functional nanomaterials, recently being researched and developed, to biomolecules have been widely researched to develop the nanobiohybrid materials because such materials have the potential to enhance and extend the function of biomolecules on a biochip. Read More

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http://dx.doi.org/10.1002/biot.201900347DOI Listing
March 2020
3.708 Impact Factor

Systematic and Comparative Evaluation of Software Programs for Template-Based Modeling of Protein Structures.

Biotechnol J 2020 Mar 4:e1900343. Epub 2020 Mar 4.

Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering (BK21 Plus Program), KAIST Institute for BioCentury, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, 34141, Republic of Korea.

Modeling protein structures is critical for understanding protein functions in various biological and biotechnological studies. Among representative protein structure modeling approaches, template-based modeling (TBM) is by far the most reliable and most widely used approach to model protein structures. However, it still remains as a challenge to select appropriate software programs for pairwise alignments and model building, two major steps of the TBM. Read More

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http://dx.doi.org/10.1002/biot.201900343DOI Listing

Synthesis of Injectable Shear-Thinning Biomaterials of Various Compositions of Gelatin and Synthetic Silicate Nanoplatelet.

Biotechnol J 2020 Feb 27:e1900456. Epub 2020 Feb 27.

Center for Minimally Invasive Therapeutics (C-MIT), University of California, Los Angeles, Los Angeles, CA, 90095, USA.

Injectable shear-thinning biomaterials (iSTBs) have great potential for in situ tissue regeneration through minimally invasive therapeutics. Previously, an iSTB was developed by combining gelatin with synthetic silicate nanoplatelets (SNPs) for potential application to hemostasis and endovascular embolization. Hence, iSTBs are synthesized by varying compositions of gelatin and SNPs to navigate their material, mechanical, rheological, and bioactive properties. Read More

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http://dx.doi.org/10.1002/biot.201900456DOI Listing
February 2020
3.708 Impact Factor

Modulation of the Purine Pathway for Riboflavin Production in Flavinogenic Recombinant Strain of the Yeast Candida famata.

Biotechnol J 2020 Feb 22:e1900468. Epub 2020 Feb 22.

Department of Molecular Biology and Biotechnology, Institute of Cell Biology, NAS of Ukraine, Drahomanov Street, 14/16, Lviv, 79005, Ukraine.

Riboflavin (vitamin B ) is an indispensable nutrient for humans and animals, since it is the precursor of the essential coenzymes flavin mononucleotide (FMN) and flavin adenine dinucleotide (FAD), involved in variety of metabolic reactions. Riboflavin is produced on commercial scale and is used for feed and food fortification purposes, and in medicine. Until recently, the mutant strains of the flavinogenic yeast Candida famata were used in industry for riboflavin production. Read More

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http://dx.doi.org/10.1002/biot.201900468DOI Listing
February 2020

A Critical Review of Genome Editing and Synthetic Biology Applications in Metabolic Engineering of Microalgae and Cyanobacteria.

Biotechnol J 2020 Feb 20:e1900228. Epub 2020 Feb 20.

Department of Chemical Engineering, National Cheng Kung University, Tainan, 701, Taiwan.

Being the green gold of the future, microalgae and cyanobacteria have recently attracted considerable interest worldwide, for their metabolites such as lipids, protein, pigments, and bioactive compounds have immense potential for sustainable energy and pharmaceutical production capabilities. In the last decades, the efforts attended to enhance the usage of microalgae and cyanobacteria by genetic manipulation, synthetic and metabolic engineering. However, the development of photoautotrophic cell factories have rarely compared to the heterotrophic counterparts due to limited tools, bioinformatics, and multi-omics database. Read More

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http://dx.doi.org/10.1002/biot.201900228DOI Listing
February 2020

Mammalian Pericardium-Based Bioprosthetic Materials in Xenotransplantation and Tissue Engineering.

Biotechnol J 2020 Feb 20:e1900334. Epub 2020 Feb 20.

Institute for Regenerative Medicine, Sechenov First Moscow State Medical University, Moscow, 119991, Russia.

Bioprosthetic materials based on mammalian pericardium tissue are the gold standard in reconstructive surgery. Their application range covers repair of rectovaginal septum defects, abdominoplastics, urethroplasty, duraplastics, maxillofacial, ophthalmic, thoracic and cardiovascular reconstruction, etc. However, a number of factors contribute to the success of their integration into the host tissue including structural organization, mechanical strength, biocompatibility, immunogenicity, surface chemistry, and biodegradability. Read More

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http://dx.doi.org/10.1002/biot.201900334DOI Listing
February 2020

Improvement of Intracellular Traffic System by Overexpression of KDEL Receptor 1 in Antibody-Producing CHO Cells.

Biotechnol J 2020 Feb 19:e1900352. Epub 2020 Feb 19.

Graduate School of Engineering , Osaka University, 2-1 Yamadaoka, Suita, Osaka, 565-0871, Japan.

The localization of soluble endoplasmic reticulum (ER) chaperones in the cell organelle is mediated by the C-terminal KDEL (lysine, aspartic acid, glutamic acid and leucine) motif. This motif is recognized by the KDEL receptor, a seven-transmembrane protein that cycles between the ER and cis-Golgi to capture missorted KDEL chaperones from post-ER compartments in a pH-dependent manner. The KDEL receptor's target chaperones have a substantial role in protein folding and assembly. Read More

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http://dx.doi.org/10.1002/biot.201900352DOI Listing
February 2020

Emerging Trends in Microfluidics Based Devices.

Biotechnol J 2020 May 24;15(5):e1900279. Epub 2020 Feb 24.

Department of Biotechnology, Delhi Technological University, Shahbad Daulatpur, Delhi, 110042, India.

One of the major challenges for scientists and engineers today is to develop technologies for the improvement of human health in both developed and developing countries. However, the need for cost-effective, high-performance diagnostic techniques is very crucial for providing accessible, affordable, and high-quality healthcare devices. In this context, microfluidic-based devices (MFDs) offer powerful platforms for automation and integration of complex tasks onto a single chip. Read More

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http://dx.doi.org/10.1002/biot.201900279DOI Listing

Next-Generation Microbial Workhorses: Comparative Genomic Analysis of Fast-Growing Vibrio Strains Reveals Their Biotechnological Potential.

Biotechnol J 2020 May 1;15(5):e1900499. Epub 2020 Mar 1.

State Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic and Developmental Sciences and School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, P. R. China.

Vibrio is a recognized fast-growing bacterial genus, which is considered to be attractive for the development of next-generation biotechnological workhorses. Here, three Vibrio strains FA1, FA2, and FA3, capable of growing rapidly in cost-effective media, are isolated and systematically evaluated. Genome sequencing and comparative genomic analyses are performed to reveal the underlying genetic differences between the strains and estimate their biotechnological potential. Read More

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http://dx.doi.org/10.1002/biot.201900499DOI Listing

Synthetic sRNA-Based Engineering of Escherichia coli for Enhanced Production of Full-Length Immunoglobulin G.

Biotechnol J 2020 May 24;15(5):e1900363. Epub 2020 Feb 24.

Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering (MOE) , School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300350, P. R. China.

Production of monoclonal antibodies (mAbs) receives considerable attention in the pharmaceutical industry. There has been an increasing interest in the expression of mAbs in Escherichia coli for analytical and therapeutic applications in recent years. Here, a modular synthetic biology approach is developed to rationally engineer E. Read More

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http://dx.doi.org/10.1002/biot.201900363DOI Listing

Quorum Sensing System Used as a Tool in Metabolic Engineering.

Biotechnol J 2020 Feb 8:e1900360. Epub 2020 Feb 8.

State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing, Chaoyang, 100029, China.

Quorum sensing (QS) is a ubiquitous cell-cell communication mechanism in microbes that coordinates population-level cell behaviors, such as biofilm production, virulence, swarming motility, and bacterial persistence. Efforts to engineer QS systems to take part in metabolic network regulation represent a promising strategy for synthetic biology and pathway engineering. Recently, design, construction, and implementation of QS circuits for programmed control of bacterial phenotypes and metabolic pathways have gained much attention, but have not been reviewed recently. Read More

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http://dx.doi.org/10.1002/biot.201900360DOI Listing
February 2020
3.708 Impact Factor

Cancer Liquid Biopsy Using Integrated Microfluidic Exosome Analysis Platforms.

Biotechnol J 2020 May 20;15(5):e1900225. Epub 2020 Feb 20.

Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education, School of Life Sciences, Jilin University, Changchun, 130012, China.

Liquid biopsies serve as both powerful noninvasive diagnostic tools for early cancer screening and prognostic tools for monitoring cancer progression and treatment efficacy. Exosomes are promising biomarkers for liquid biopsies, since these nano-sized extracellular vesicles (EVs) enrich proteins, lipids, mRNAs, and miRNAs from cells of origin, including cancer cells. Although exosomes are abundantly present in various bodily fluids, conventional exosome isolation and detection methods that rely on benchtop equipment are time-consuming, expensive, and involve complicated non-portable procedures. Read More

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http://dx.doi.org/10.1002/biot.201900225DOI Listing

An Automatable Hydrogel Culture Platform for Evaluating Efficacy of Antibody-Based Therapeutics in Overcoming Chemoresistance.

Biotechnol J 2020 May 24;15(5):e1900439. Epub 2020 Feb 24.

CellSpring AG, ETH Zürich ieLab, Zürich, Switzerland.

The microenvironment plays a major role in conferring chemoresistance to cancer cells. In order to better inform clinical response to chemoresistance, preclinical models that recapitulate its hallmark features are needed to enable screening for resistance-specific therapeutic targets. A novel platform for seeding cancer cells in 3D hydrogels is presented utilizing derivatives of chitosan and alginate that, critically, is amenable to high throughput screening: cell seeding in hydrogels, media changes, dosing of anticancer compounds, and cell viability assays are all automated using a standard and commercially available liquid handling robot. Read More

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http://dx.doi.org/10.1002/biot.201900439DOI Listing

Metal-Organic Frameworks for Photodynamic Therapy: Emerging Synergistic Cancer Therapy.

Biotechnol J 2020 Feb 5:e1900382. Epub 2020 Feb 5.

State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, P. R. China.

Photodynamic therapy (PDT) conducted by photosensitizers producing cytotoxic reactive oxygen species (ROS) under light irradiation is widely used in cancer treatment. A great number of photoactive nanoscale metal-organic frameworks (NMOFs) have been prepared for PDT. With the development of biomedicine and nanotechnology, many synergistic cancer therapies have emerged. Read More

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http://dx.doi.org/10.1002/biot.201900382DOI Listing
February 2020

Comparison of Modeling Methods for DoE-Based Holistic Upstream Process Characterization.

Biotechnol J 2020 May 17;15(5):e1900551. Epub 2020 Feb 17.

Department of Biotechnology, University of Natural Resources and Life Sciences, Vienna, 1190, Austria.

Upstream bioprocess characterization and optimization are time and resource-intensive tasks. Regularly in the biopharmaceutical industry, statistical design of experiments (DoE) in combination with response surface models (RSMs) are used, neglecting the process trajectories and dynamics. Generating process understanding with time-resolved, dynamic process models allows to understand the impact of temporal deviations, production dynamics, and provides a better understanding of the process variations that stem from the biological subsystem. Read More

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http://dx.doi.org/10.1002/biot.201900551DOI Listing