2,120 results match your criteria Biotechnology For Biofuels[Journal]


Biosynthesis of acetylacetone inspired by its biodegradation.

Biotechnol Biofuels 2020 15;13:88. Epub 2020 May 15.

1CAS Key Laboratory of Biobased Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, 266101 China.

Background: Acetylacetone is a commercially bulk chemical with diverse applications. However, the traditional manufacturing methods suffer from many drawbacks such as multiple steps, harsh conditions, low yield, and environmental problems, which hamper further applications of petrochemical-based acetylacetone. Compared to conventional chemical methods, biosynthetic methods possess advantages such as being eco-friendly, and having mild conditions, high selectivity and low potential costs. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13068-020-01725-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7226712PMC

Hydrophobic pore space constituted in macroporous ZIF-8 for lipase immobilization greatly improving lipase catalytic performance in biodiesel preparation.

Biotechnol Biofuels 2020 13;13:86. Epub 2020 May 13.

1Key Laboratory for Industrial Biocatalysis, Ministry of Education, Department of Chemical Engineering, Tsinghua University, Beijing, 100084 China.

Background: During lipase-mediated biodiesel production, by-product glycerol adsorbing on immobilized lipase is a common trouble that hinders enzymatic catalytic activity in biodiesel production process. In this work, we built a hydrophobic pore space in macroporous ZIF-8 (named as M-ZIF-8) to accommodate lipase so that the generated glycerol would be hard to be adsorbed in such hydrophobic environment. The performance of the immobilized lipase in biodiesel production as well as its characteristics for glycerol adsorption were systematically studied. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13068-020-01724-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7222533PMC

Repeated batches as a strategy for high 2G ethanol production from undetoxified hemicellulose hydrolysate using immobilized cells of recombinant in a fixed-bed reactor.

Biotechnol Biofuels 2020 11;13:85. Epub 2020 May 11.

1Department of Chemical Engineering, Federal University of São Carlos, Rodovia Washington Luís, km 235, 13565-905 São Carlos, SP Brazil.

Background: The search for sustainable energy sources has become a worldwide issue, making the development of efficient biofuel production processes a priority. Immobilization of second-generation (2G) xylose-fermenting strains is a promising approach to achieve economic viability of 2G bioethanol production from undetoxified hydrolysates through operation at high cell load and mitigation of inhibitor toxicity. In addition, the use of a fixed-bed reactor can contribute to establish an efficient process because of its distinct advantages, such as high conversion rate per weight of biocatalyst and reuse of biocatalyst. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13068-020-01722-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7216711PMC

RRNPP-type quorum-sensing systems regulate solvent formation, sporulation and cell motility in .

Biotechnol Biofuels 2020 8;13:84. Epub 2020 May 8.

1Department of Biosystems Engineering, Auburn University, 350 Mell Street, Auburn, AL 36849 USA.

Background: N1-4 (HMT) is a strictly anaerobic, spore-forming Gram-positive bacterium capable of hyper-butanol production through the well-known acetone-butanol-ethanol fermentation process. Recently, five putative RRNPP-type QSSs (here designated as QSS1 to QSS5) were predicted in this bacterial strain, each of which comprises a putative RRNPP-type regulator (QssR1 to QssR5) and a cognate signaling peptide precursor (QssP1 to QssP5). In addition, both proteins are encoded by the same operon. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13068-020-01723-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7206700PMC

Acyl-lipid desaturases and Vipp1 cooperate in cyanobacteria to produce novel omega-3 PUFA-containing glycolipids.

Biotechnol Biofuels 2020 6;13:83. Epub 2020 May 6.

3Department of Physiology and Pharmacology, Wake Forest School of Medicine, Winston-Salem, NC 27157 USA.

Background: Dietary omega-3 (n-3), long chain (LC-, ≥ 20 carbons), polyunsaturated fatty acids (PUFAs) derived largely from marine animal sources protect against inflammatory processes and enhance brain development and function. With the depletion of natural stocks of marine animal sources and an increasing demand for n-3 LC-PUFAs, alternative, sustainable supplies are urgently needed. As a result, n-3 18-carbon and LC-PUFAs are being generated from plant or algal sources, either by engineering new biosynthetic pathways or by augmenting existing systems. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13068-020-01719-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7203895PMC

Construction and analysis of an artificial consortium based on the fast-growing cyanobacterium UTEX 2973 to produce the platform chemical 3-hydroxypropionic acid from CO.

Biotechnol Biofuels 2020 6;13:82. Epub 2020 May 6.

1Laboratory of Synthetic Microbiology, School of Chemical Engineering & Technology, Tianjin University, Tianjin, 300072 People's Republic of China.

Background: Cyanobacterial carbohydrates, such as sucrose, have been considered as potential renewable feedstock to support the production of fuels and chemicals. However, the separation and purification processes of these carbohydrates will increase the production cost of chemicals. Co-culture fermentation has been proposed as an efficient and economical way to utilize these cyanobacterial carbohydrates. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13068-020-01720-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7201998PMC

Microbial community dynamics in mesophilic and thermophilic batch reactors under methanogenic, phenyl acid-forming conditions.

Biotechnol Biofuels 2020 6;13:81. Epub 2020 May 6.

1Department of Microbiology, Universität Innsbruck, Technikerstraße 25d, 6020 Innsbruck, Austria.

Background: Proteinaceous wastes exhibit high theoretical methane yields and their residues are considered valuable fertilisers. The routine anaerobic degradation of proteins often raises problems like high aromatic compound concentrations caused by the entry of aromatic amino acids into the system. A profound investigation of the consequences of aromatic compound exposure on various microorganisms, which cascade-like and interdependently degrade complex molecules to biogas, is still pending. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13068-020-01721-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7201606PMC

Enhanced rhamnolipids production using a novel bioreactor system based on integrated foam-control and repeated fed-batch fermentation strategy.

Biotechnol Biofuels 2020 24;13:80. Epub 2020 Apr 24.

1State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Puzhu South Road 30#, Nanjing, 211816 People's Republic of China.

Background: Rhamnolipids are the best known microbial-derived biosurfactants, which has attracted great interest as potential ''green" alternative for synthetic surfactants. However, rhamnolipids are the major contributors to severe foam problems, which greatly inhibit the economics of industrial-scale production. In this study, a novel foam-control system was established for ex situ dealing with the massive overflowing foam. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13068-020-01716-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7181576PMC

Improvement of sabinene tolerance of using adaptive laboratory evolution and omics technologies.

Biotechnol Biofuels 2020 24;13:79. Epub 2020 Apr 24.

1CAS Key Laboratory of Biobased Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, No. 189 Songling Road, Laoshan District, Qingdao, 266101 China.

Background: Biosynthesis of sabinene, a bicyclic monoterpene, has been accomplished in engineered microorganisms by introducing heterologous pathways and using renewable sugar as a carbon source. However, the efficiency and titers of this method are limited by the low host tolerance to sabinene (in both eukaryotes and prokaryotes).

Results: In this study, BL21(DE3) was selected as the strain for adaptive laboratory evolution. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13068-020-01715-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7181518PMC

Improved lipid productivity in in nitrogen-replete conditions by selection of pale green mutants.

Biotechnol Biofuels 2020 21;13:78. Epub 2020 Apr 21.

1Dipartimento di Biotecnologie, Università degli Studi di Verona, Strada le Grazie 15, 37134 Verona, Italy.

Background: is a photosynthetic unicellular microalgae considered one of the most interesting marine algae to produce biofuels and food additive due to its rapid growth rate and high lipid accumulation. Although microalgae are attractive platforms for solar energy bioconversion, the overall efficiency of photosynthesis is reduced due to the steep light gradient in photobioreactors. Moreover, accumulation of lipids in microalgae for biofuels production is usually induced in a two-phase cultivation process by nutrient starvation, with additional time and costs associated. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13068-020-01718-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7175523PMC

High-solids enzymatic hydrolysis of ball-milled corn stover with reduced slurry viscosity and improved sugar yields.

Biotechnol Biofuels 2020 20;13:77. Epub 2020 Apr 20.

College of Engineering, China Agricultural University (East Campus), P.O. Box 191, 17 Qing-Hua-Dong-Lu, Hai-Dian District, Beijing, 100083 People's Republic of China.

Background: High-solids enzymatic hydrolysis has attracted increasing attentions for the production of bioethanol from lignocellulosic biomass with its advantages of high product concentration, water saving, and low energy and capital costs. However, the increase of solids content would worsen the rheological properties, resulting in heat/mass transfer limitation and higher mixing energy. To address these issues, ball milling was applied to corn stover prior to enzymatic hydrolysis, and the rheological behaviors and digestibility of ball-milled corn stover under high-solids loading were investigated. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13068-020-01717-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7171840PMC

From Eat to trEat: engineering the mitochondrial Eat1 enzyme for enhanced ethyl acetate production in .

Biotechnol Biofuels 2020 19;13:76. Epub 2020 Apr 19.

2Bioprocess Engineering, Wageningen University and Research, Droevendaalsesteeg 1, 6708 PB Wageningen, The Netherlands.

Background: Genetic engineering of microorganisms has become a common practice to establish microbial cell factories for a wide range of compounds. Ethyl acetate is an industrial solvent that is used in several applications, mainly as a biodegradable organic solvent with low toxicity. While ethyl acetate is produced by several natural yeast species, the main mechanism of production has remained elusive until the discovery of Eat1 in . Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13068-020-01711-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7168974PMC

Evidence for ligninolytic activity of the ascomycete fungus .

Biotechnol Biofuels 2020 16;13:75. Epub 2020 Apr 16.

1Laboratory of Food Chemistry, Wageningen University and Research, Bornse Weilanden 9, 6708 WG Wageningen, The Netherlands.

Background: The ascomycete fungus has been appreciated for its targeted carbohydrate-active enzymatic arsenal. As a late colonizer of herbivorous dung, the fungus acts specifically on the more recalcitrant fraction of lignocellulose and this lignin-rich biotope might have resulted in the evolution of ligninolytic activities. However, the lignin-degrading abilities of the fungus have not been demonstrated by chemical analyses at the molecular level and are, thus far, solely based on genome and secretome predictions. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13068-020-01713-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7161253PMC

Carotenoids and lipid production from cultured in tea waste hydrolysate.

Biotechnol Biofuels 2020 16;13:74. Epub 2020 Apr 16.

1Engineering Research Center of Industrial Microbiology of Ministry of Education, College of Life Sciences, Fujian Normal University, Fuzhou, 350117 Fujian China.

Background: In this study, renewable tea waste hydrolysate was used as a sole carbon source for carotenoids and lipid production. A novel mutant strain, RM18, was isolated through atmospheric and room-temperature plasma mutagenesis and continuous domestication in tea waste hydrolysate from ACCC20341.

Results: RM18 produced a larger biomass and more carotenoids and α-linolenic acid compared with the control strain cultured in tea waste hydrolysate. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13068-020-01712-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7161300PMC

Novel insights into salinity-induced lipogenesis and carotenogenesis in the oleaginous astaxanthin-producing alga : a multi-omics study.

Biotechnol Biofuels 2020 16;13:73. Epub 2020 Apr 16.

Laboratory for Algae Biotechnology & Innovation, College of Engineering, Peking University, Beijing, 100871 China.

Background: , a freshwater alga capable of synthesizing both triacylglycerol (TAG) and astaxanthin, has been receiving increasing attention as a leading candidate producer. While the mechanism of oleaginousness and/or carotenogenesis has been studied under such induction conditions as nitrogen deprivation, high light and glucose feeding, it remains to be elucidated in response to salt stress, a condition critical for reducing freshwater footprint during algal production processes.

Results: Firstly, the effect of salt concentrations on growth, lipids and carotenoids was examined for , and 0. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13068-020-01714-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7161124PMC

Evaluation of organic fractions of municipal solid waste as renewable feedstock for succinic acid production.

Biotechnol Biofuels 2020 15;13:72. Epub 2020 Apr 15.

1Department of Food Science and Human Nutrition, Agricultural University of Athens, Iera Odos 75, 118 55 Athens, Greece.

Background: Despite its high market potential, bio-based succinic acid production experienced recently a declining trend because the initial investments did not meet the expectations for rapid market growth. Thus, reducing the succinic acid production cost is imperative to ensure industrial implementation.

Results: Succinic acid production has been evaluated using hydrolysates from the organic fraction of municipal solid waste (OFMSW) collected from MSW treatment plants. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13068-020-01708-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7160979PMC

Strategies for the production of biochemicals in bioenergy crops.

Biotechnol Biofuels 2020 15;13:71. Epub 2020 Apr 15.

1Joint BioEnergy Institute, Emeryville, CA 94608 USA.

Industrial crops are grown to produce goods for manufacturing. Rather than food and feed, they supply raw materials for making biofuels, pharmaceuticals, and specialty chemicals, as well as feedstocks for fabricating fiber, biopolymer, and construction materials. Therefore, such crops offer the potential to reduce our dependency on petrochemicals that currently serve as building blocks for manufacturing the majority of our industrial and consumer products. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13068-020-01707-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7158082PMC

Distribution and diversity of olefins and olefin-biosynthesis genes in Gram-positive bacteria.

Biotechnol Biofuels 2020 15;13:70. Epub 2020 Apr 15.

Chair of Microbiology, Technical University of Munich, Emil-Ramann-Str. 4, 85354 Freising-Weihenstephan, Germany.

Background: The natural production of olefins (unsaturated aliphatic hydrocarbons) by certain bacterial genera represents an alternative and sustainable source of biofuels and lubricant components. The biochemical steps of olefin biosynthesis via the ole pathway encoded by have been unraveled recently, and the occurrence of olefins has been reported for several Gram-negative and Gram-positive bacteria. However, the distribution and diversity of olefins among the Gram-positive bacteria has not been studied in detail. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13068-020-01706-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7158056PMC

Succession of physiological stages hallmarks the transcriptomic response of the fungus to lignocellulose.

Biotechnol Biofuels 2020 13;13:69. Epub 2020 Apr 13.

1School of Life Sciences, University of Nottingham, University Park, Nottingham, NG7 2RD UK.

Background: Understanding how fungi degrade lignocellulose is a cornerstone of improving renewables-based biotechnology, in particular for the production of hydrolytic enzymes. Considerable progress has been made in investigating fungal degradation during time-points where CAZyme expression peaks. However, a robust understanding of the fungal survival strategies over its life time on lignocellulose is thereby missed. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13068-020-01702-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7155255PMC

Investigation of a thermostable multi-domain xylanase-glucuronoyl esterase enzyme from incorporating multiple carbohydrate-binding modules.

Biotechnol Biofuels 2020 11;13:68. Epub 2020 Apr 11.

1Division of Industrial Biotechnology, Department of Biology and Biological Engineering, Chalmers University of Technology, 412 96 Gothenburg, Sweden.

Background: Efficient degradation of lignocellulosic biomass has become a major bottleneck in industrial processes which attempt to use biomass as a carbon source for the production of biofuels and materials. To make the most effective use of the source material, both the hemicellulosic as well as cellulosic parts of the biomass should be targeted, and as such both hemicellulases and cellulases are important enzymes in biorefinery processes. Using thermostable versions of these enzymes can also prove beneficial in biomass degradation, as they can be expected to act faster than mesophilic enzymes and the process can also be improved by lower viscosities at higher temperatures, as well as prevent the introduction of microbial contamination. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13068-020-01709-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7151638PMC

Effect of residual extractable lignin on acetone-butanol-ethanol production in SHF and SSF processes.

Biotechnol Biofuels 2020 10;13:67. Epub 2020 Apr 10.

3Department of Chemical and Environmental Engineering, University of Cincinnati, Cincinnati, OH 45221 USA.

Background: Lignin plays an important role in biochemical conversion of biomass to biofuels. A significant amount of lignin is precipitated on the surface of pretreated substrates after organosolv pretreatment. The effect of this residual lignin on enzymatic hydrolysis has been well understood, however, their effect on subsequent ABE fermentation is still unknown. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13068-020-01710-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7149896PMC

Enzymatic conversion reactions of 5-hydroxymethylfurfural (HMF) to bio-based - 2,5-furandicarboxylic acid (FDCA) with air: mechanisms, pathways and synthesis selectivity.

Biotechnol Biofuels 2020 10;13:66. Epub 2020 Apr 10.

Department of Catalysis and Chemical Reaction Engineering, National Institute of Chemistry, Hajdrihova 19, 1000 Ljubljana, Slovenia.

Background: 2,5-Furandicarboxylic acid (FDCA) is one of the top biomass-derived value-added chemicals. It can be produced from fructose and other C6 sugars via formation of 5-hydroxymethilfurfural (HMF) intermediate. Most of the chemical methods for FDCA production require harsh conditions, thus as an environmentally friendly alternative, an enzymatic conversion process can be applied. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13068-020-01705-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7149886PMC

Multilevel optimisation of anaerobic ethyl acetate production in engineered .

Biotechnol Biofuels 2020 7;13:65. Epub 2020 Apr 7.

1Bioprocess Engineering, Wageningen University and Research, Droevendaalsesteeg 1, 6708 PB Wageningen, The Netherlands.

Background: Ethyl acetate is a widely used industrial solvent that is currently produced by chemical conversions from fossil resources. Several yeast species are able to convert sugars to ethyl acetate under aerobic conditions. However, performing ethyl acetate synthesis anaerobically may result in enhanced production efficiency, making the process economically more viable. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13068-020-01703-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7137189PMC

Mechanistic insights into pH-dependent H photoproduction in bisulfite-treated cells.

Biotechnol Biofuels 2020 6;13:64. Epub 2020 Apr 6.

Shanghai Key Laboratory of Plant Molecular Sciences, College of Life Sciences, Shanghai Normal University, Guilin Road 100, Shanghai, 200234 China.

Background: Bisulfite addition is an important H photoproduction strategy that removes O and activates hydrogenase. The pH values of cell cultures can change the ratio of bisulfite to sulfite, which may affect H photoproduction. However, little is known regarding the pH effect of bisulfite addition on H photoproduction and relevant underlying mechanism. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13068-020-01704-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7132995PMC

Semi-quantitative determination of ash element content for freeze-dried, defatted, sulfated and pyrolysed biomass of sp.

Biotechnol Biofuels 2020 1;13:63. Epub 2020 Apr 1.

1Centro de Engenharia, Modelagem e Ciência Sociais Aplicadas, Universidade Federal do ABC, Avenida dos Estados, 5001, Bairro Bangu, Santo André, SP 09210-580 Brazil.

Background: Energy demand by mankind has become one of the most important aspects of our society. A promising technology that seeks to provide part of the energy demand and to obtain high-value products is the thermochemical conversion of microalgae biomass. Inorganic species presented in microalgae biomass may act as catalysts for thermochemical reactions and are responsible for notorious ash-related issues during thermochemical decomposition. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13068-020-01699-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7115075PMC

Engineering of for enhanced degradation of lignocellulosic biomass by truncation of the cellulase activator ACE3.

Biotechnol Biofuels 2020 1;13:62. Epub 2020 Apr 1.

1State Key Lab of Bioreactor Engineering, East China University of Science and Technology, P.O.B. 311, 130 Meilong Road, Shanghai, 200237 China.

Background: The filamentous fungus is a major workhorse employed to produce cellulase, which hydrolyzes lignocellulosic biomass for the production of cellulosic ethanol and bio-based products. However, the economic efficiency of biorefineries is still low.

Results: In this study, the truncation of cellulase activator ACE3 was identified and characterized in classical mutant NG14 and its direct descendants for the first time. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13068-020-01701-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7110754PMC
April 2020
6.044 Impact Factor

Biotransformation of keratin waste to amino acids and active peptides based on cell-free catalysis.

Biotechnol Biofuels 2020 1;13:61. Epub 2020 Apr 1.

1School of Biotechnology, Jiangnan University, 1800 Lihu Road, Wuxi, 214122 China.

Background: Keratin is the primary constituent of the vertebrate epidermis and epidermal appendages, as well as the main waste product generated during poultry processing from feathers, hair, scales, nails, etc. Keratin is generally hard, stubborn and difficult to hydrolyze; however, it is also inexpensive and contains more than 85% protein. Currently, tens of millions of tons of keratin waste are produced each year worldwide; however, no effective methods for the recovery of keratin waste have been reported thus far, making such research urgent. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13068-020-01700-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7110813PMC

Multimodular fused acetyl-feruloyl esterases from soil and gut Bacteroidetes improve xylanase depolymerization of recalcitrant biomass.

Biotechnol Biofuels 2020 31;13:60. Epub 2020 Mar 31.

1Division of Industrial Biotechnology, Department of Biology and Biological Engineering, Chalmers University of Technology, 412 96 Gothenburg, Sweden.

Background: Plant biomass is an abundant and renewable carbon source that is recalcitrant towards both chemical and biochemical degradation. Xylan is the second most abundant polysaccharide in biomass after cellulose, and it possesses a variety of carbohydrate substitutions and non-carbohydrate decorations which can impede enzymatic degradation by glycoside hydrolases. Carbohydrate esterases are able to cleave the ester-linked decorations and thereby improve the accessibility of the xylan backbone to glycoside hydrolases, thus improving the degradation process. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13068-020-01698-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7110780PMC

Modeling ethanol production through gas fermentation: a biothermodynamics and mass transfer-based hybrid model for microbial growth in a large-scale bubble column bioreactor.

Biotechnol Biofuels 2020 27;13:59. Epub 2020 Mar 27.

2Department of Biotechnology, Faculty of Applied Sciences, Delft University of Technology, Van der Maasweg 9, 2629 HZ Delft, The Netherlands.

Background: Ethanol production through fermentation of gas mixtures containing CO, CO and H has just started operating at commercial scale. However, quantitative schemes for understanding and predicting productivities, yields, mass transfer rates, gas flow profiles and detailed energy requirements have been lacking in literature; such are invaluable tools for process improvements and better systems design. The present study describes the construction of a hybrid model for simulating ethanol production inside a 700 m bubble column bioreactor fed with gas of two possible compositions, i. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13068-020-01695-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7102449PMC

Constraints and advances in high-solids enzymatic hydrolysis of lignocellulosic biomass: a critical review.

Biotechnol Biofuels 2020 23;13:58. Epub 2020 Mar 23.

2Bioethanol Laboratory, Department of Biochemistry, Chemistry Institute, Federal University of Rio de Janeiro, Rio de Janeiro, RJ 21941-909 Brazil.

The industrial production of sugar syrups from lignocellulosic materials requires the conduction of the enzymatic hydrolysis step at high-solids loadings (i.e., with over 15% solids [w/w] in the reaction mixture). Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13068-020-01697-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7092515PMC

Lipase immobilized on functionalized superparamagnetic few-layer graphene oxide as an efficient nanobiocatalyst for biodiesel production from bio-oil.

Biotechnol Biofuels 2020 20;13:57. Epub 2020 Mar 20.

3Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran.

Background: Microalgae, due to its well-recognized advantages have gained renewed interest as potentially good feedstock for biodiesel. Production of fatty acid methyl esters (FAMEs) as a type of biodiesel was carried out from bio-oil. Biodiesel was produced in the presence of nano-biocatalysts composed of immobilized lipase on functionalized superparamagnetic few-layer graphene oxide via a transesterification reaction. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13068-020-01688-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7082915PMC

Heteroexpression of - improved switchgrass biomass yield and feedstock quality by repression of .

Biotechnol Biofuels 2020 19;13:56. Epub 2020 Mar 19.

2College of Grassland Science and Technology, China Agricultural University, Beijing, 100193 People's Republic of China.

Background: Switchgrass ( L.), a C perennial grass, has been recognized as one of the most potentially important lignocellulose biofuel crops. MicroRNA319 (miR319) plays a key role in plant development, abiotic resistance, and cell wall biosynthesis by repressing expression of its target genes. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13068-020-01693-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7081615PMC

Biodiesel and flavor compound production using a novel promiscuous cold-adapted SGNH-type lipase (SGNH1) from the psychrophilic bacterium .

Biotechnol Biofuels 2020 16;13:55. Epub 2020 Mar 16.

1Department of Chemistry, College of Natural Science, Sookmyung Women's University, Seoul, 04310 South Korea.

Background: Biodiesel and flavor compound production using enzymatic transesterification by microbial lipases provides mild reaction conditions and low energy cost compared to the chemical process. SGNH-type lipases are very effective catalysts for enzymatic transesterification due to their high reaction rate, great stability, relatively small size for convenient genetic manipulations, and ease of immobilization. Hence, it is highly important to identify novel SGNH-type lipases with high catalytic efficiencies and good stabilities. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13068-020-01696-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7074997PMC

Calcineurin signaling pathway influences biofilm formation by affecting hydrophobicity and cell wall integrity.

Biotechnol Biofuels 2020 16;13:54. Epub 2020 Mar 16.

1National Engineering Research Center for Biotechnology, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing, China.

Background: Biofilms, as a kind of fixed-cell community, can greatly improve industrial fermentation efficiency in immobilized fermentation, but the regulation process is still unclear, which restricts their application. Ca was reported to be a key factor affecting biofilm formation. However, the effect of Ca on biofilm structure and microbiology was yet only studied in bacteria. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13068-020-01692-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7075038PMC

Microbial insights of enhanced anaerobic conversion of syngas into volatile fatty acids by co-fermentation with carbohydrate-rich synthetic wastewater.

Biotechnol Biofuels 2020 16;13:53. Epub 2020 Mar 16.

2Shanghai Key Laboratory of Atmospheric Particle Pollution and Prevention (LAP3), Department of Environment Science and Engineering, Fudan University, Shanghai, 200433 China.

Background: The co-fermentation of syngas (mainly CO, H and CO) and different concentrations of carbohydrate/protein synthetic wastewater to produce volatile fatty acids (VFAs) was conducted in the present study.

Results: It was found that co-fermentation of syngas with carbohydrate-rich synthetic wastewater could enhance the conversion efficiency of syngas and the most efficient conversion of syngas was obtained by co-fermentation of syngas with 5 g/L glucose, which resulted in 25% and 43% increased conversion efficiencies of CO and H, compared to syngas alone. The protein-rich synthetic wastewater as co-substrate, however, had inhibition on syngas conversion due to the presence of high concentration of NH-N (> 900 mg/L) produced from protein degradation. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13068-020-01694-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7076986PMC
March 2020
6.044 Impact Factor

Establishing an enzyme cascade for one-pot production of α-olefins from low-cost triglycerides and oils without exogenous HO addition.

Biotechnol Biofuels 2020 16;13:52. Epub 2020 Mar 16.

1Shandong Provincial Key Laboratory of Synthetic Biology, CAS Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, No. 189 Songling Road, Qingdao, 266101 Shandong China.

Background: Biological α-olefins can be used as both biofuels and high value-added chemical precursors to lubricants, polymers, and detergents. The prototypic CYP152 peroxygenase family member OleT from sp. ATCC 8456 catalyzes a single-step decarboxylation of free fatty acids (FFAs) to form α-olefins using HO as a cofactor, thus attracting much attention since its discovery. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13068-020-01684-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7075034PMC

Enzymatic production of 4--methyl d-glucaric acid from hardwood xylan.

Biotechnol Biofuels 2020 13;13:51. Epub 2020 Mar 13.

1Department of Chemical Engineering and Applied Chemistry, University of Toronto, Toronto, ON Canada.

Background: Dicarboxylic acids offer several applications in detergent builder and biopolymer fields. One of these acids, 4--methyl d-glucaric acid, could potentially be produced from glucuronoxylans, which are a comparatively underused fraction of wood and agricultural biorefineries.

Results: Accordingly, an enzymatic pathway was developed that combines AxyAgu115A, a GH115 α-glucuronidase from , and GOOX, an AA7 gluco-oligosaccharide oxidase from , to produce this bio-based chemical from glucuronoxylan. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13068-020-01691-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7071571PMC

Gene targets for engineering osmotolerance in .

Biotechnol Biofuels 2020 13;13:50. Epub 2020 Mar 13.

1BioEnergy Science Center, Oak Ridge National Laboratory, Oak Ridge, TN USA.

Background: , a promising biocatalyst being developed for use in consolidated bioprocessing of lignocellulosic materials to ethanol, grows poorly and has reduced conversion at elevated medium osmolarities. Increasing tolerance to elevated fermentation osmolarities is desired to enable performance necessary of a consolidated bioprocessing (CBP) biocatalyst.

Results: Two strains of showing growth phenotypes in elevated osmolarity conditions were identified. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13068-020-01690-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7071700PMC

Isopod holobionts as promising models for lignocellulose degradation.

Biotechnol Biofuels 2020 13;13:49. Epub 2020 Mar 13.

Laboratoire Ecologie et Biologie des Interactions-UMR CNRS 7267, Ecologie et Biologie des Interactions-Bâtiment B8-B35, Université de Poitiers, 5 rue Albert Turpin, TSA 51106, 86073 Poitiers Cedex 9, France.

Background: Isopods have colonized all environments, partly thanks to their ability to decompose the organic matter. Their enzymatic repertoire, as well as the one of their associated microbiota, has contributed to their colonization success. Together, these holobionts have evolved several interesting life history traits to degrade the plant cell walls, mainly composed of lignocellulose. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13068-020-01683-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7071664PMC

Genomic and transcriptomic analysis of reveals the genetic determinants for its xylose-converting capacity.

Biotechnol Biofuels 2020 12;13:48. Epub 2020 Mar 12.

1Division of Industrial Biotechnology, Department of Biology and Biological Engineering, Chalmers University of Technology, Gothenburg, Sweden.

Background: An economically viable production of biofuels and biochemicals from lignocellulose requires microorganisms that can readily convert both the cellulosic and hemicellulosic fractions into product. The yeast displays a high capacity for uptake and conversion of several lignocellulosic sugars including the abundant pentose d-xylose, an underutilized carbon source since most industrially relevant microorganisms cannot naturally ferment it. Thus, constitutes an important source of knowledge and genetic information that could be transferred to industrial microorganisms such as to improve their capacity to ferment lignocellulose-derived xylose. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13068-020-1663-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7068945PMC

meal hydrolysate as sustainable biomass for the production of carotenoids by .

Biotechnol Biofuels 2020 12;13:47. Epub 2020 Mar 12.

1Department of Biotechnology and Biosciences, University of Milano-Bicocca, Piazza della Scienza 2, 20126 Milan, Italy.

Background: As the circular economy advocates a near total waste reduction, the industry has shown an increased interest toward the exploitation of various residual biomasses. The origin and availability of biomass used as feedstock strongly affect the sustainability of biorefineries, where it is converted in energy and chemicals. Here, we explored the valorization of meal, the leftover residue from oil extraction. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13068-020-01682-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7066749PMC

Synergy at work: linking the metabolism of two lactic acid bacteria to achieve superior production of 2-butanol.

Biotechnol Biofuels 2020 11;13:45. Epub 2020 Mar 11.

National Food Institute, Technical University of Denmark, Kemitorvet, Building 201, 2800 Kgs. Lyngby, Denmark.

Background: The secondary alcohol 2-butanol has many important applications, e.g., as a solvent. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13068-020-01689-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7065357PMC

Use of the lignocellulose-degrading bacterium to assess recalcitrance and conversion of wild-type and transgenic poplar.

Biotechnol Biofuels 2020 11;13:43. Epub 2020 Mar 11.

1Department of Chemical and Biomolecular Engineering, North Carolina State University, EB-1, 911 Partners Way, Raleigh, NC 27695-7905 USA.

Background: Biological conversion of lignocellulosic biomass is significantly hindered by feedstock recalcitrance, which is typically assessed through an enzymatic digestion assay, often preceded by a thermal and/or chemical pretreatment. Here, we assay 17 lines of unpretreated transgenic black cottonwood () utilizing a lignocellulose-degrading, metabolically engineered bacterium, . The poplar lines were assessed by incubation with an engineered strain that solubilized and converted the hexose and pentose carbohydrates to ethanol and acetate. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13068-020-01675-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7065347PMC
March 2020
6.044 Impact Factor

Understanding the structural characteristics of water-soluble phenolic compounds from four pretreatments of corn stover and their inhibitory effects on enzymatic hydrolysis and fermentation.

Biotechnol Biofuels 2020 11;13:44. Epub 2020 Mar 11.

1School of Environmental and Biological Engineering, Nanjing University of Science and Technology, 200 Xiaolingwei Street, Xuanwu District, Nanjing, 210094 China.

Background: For bioethanol production from lignocellulosic biomass, phenolics derived from pretreatment have been generally considered as highly inhibitory towards enzymatic hydrolysis and fermentation. As phenolics are produced from lignin degradation during pretreatment, it is likely that the pretreatment will exert a strong impact on the structure of phenolics, resulting in varied levels of inhibition of the bioconversion process. Despite the extensive studies on pretreatment, it remains unclear how pretreatment process affects the properties of generated phenolics and how the inhibitory effect of phenolics from different pretreatment varies on enzymatic hydrolysis and fermentation. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13068-020-01686-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7065323PMC

Fine-mapping and transcriptome analysis of a candidate gene controlling plant height in L.

Biotechnol Biofuels 2020 10;13:42. Epub 2020 Mar 10.

1Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture and Rural Affairs, Wuhan, China.

Background: provides approximately 13-16% of global vegetable oil for human consumption and biodiesel production. Plant height (PH) is a key trait that affects plant architecture, seed yield and harvest index. However, the genetic mechanism of PH in is poorly understood. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13068-020-01687-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7063735PMC

Efficient bioproduction of 5-aminolevulinic acid, a promising biostimulant and nutrient, from renewable bioresources by engineered .

Biotechnol Biofuels 2020 10;13:41. Epub 2020 Mar 10.

Key Laboratory of Systems Microbial Biotechnology, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308 China.

Background: 5-Aminolevulinic acid (5-ALA) is a promising biostimulant, feed nutrient, and photodynamic drug with wide applications in modern agriculture and therapy. Considering the complexity and low yield of chemical synthesis methods, bioproduction of 5-ALA has drawn intensive attention recently. However, the present bioproduction processes use refined glucose as the main carbon source and the production level still needs further enhancement. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13068-020-01685-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7063817PMC

Metabolic and evolutionary responses of to genetic interventions aimed at improving ethanol production.

Biotechnol Biofuels 2020 10;13:40. Epub 2020 Mar 10.

1Thayer School of Engineering, Dartmouth College, Hanover, NH 03755 USA.

Background: Engineering efforts targeted at increasing ethanol by modifying the central fermentative metabolism of have been variably successful. Here, we aim to understand this variation by a multifaceted approach including genomic and transcriptomic analysis combined with chemostat cultivation and high solids cellulose fermentation. Three strain lineages comprising 16 strains total were examined. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13068-020-01680-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7063780PMC

Pathway dissection, regulation, engineering and application: lessons learned from biobutanol production by solventogenic clostridia.

Biotechnol Biofuels 2020 6;13:39. Epub 2020 Mar 6.

3State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, 214122 China.

The global energy crisis and limited supply of petroleum fuels have rekindled the interest in utilizing a sustainable biomass to produce biofuel. Butanol, an advanced biofuel, is a superior renewable resource as it has a high energy content and is less hygroscopic than other candidates. At present, the biobutanol route, employing acetone-butanol-ethanol (ABE) fermentation in species, is not economically competitive due to the high cost of feedstocks, low butanol titer, and product inhibition. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13068-020-01674-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7060580PMC

Development and characterization of a mutant with simultaneously enhanced growth and lipid production.

Biotechnol Biofuels 2020 5;13:38. Epub 2020 Mar 5.

1Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology (KAIST), 291 Daehak-ro, Yuseong-gu, Daejeon, 34141 Republic of Korea.

Background: The necessity to develop high lipid-producing microalgae is emphasized for the commercialization of microalgal biomass, which is environmentally friendly and sustainable. are one of the best industrial microalgae and have been widely studied for their lipids, including high-value polyunsaturated fatty acids (PUFAs). Many reports on the genetic and biological engineering of to improve their growth and lipid contents have been published. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13068-020-01681-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7057510PMC

The HO-dependent activity of a fungal lytic polysaccharide monooxygenase investigated with a turbidimetric assay.

Biotechnol Biofuels 2020 5;13:37. Epub 2020 Mar 5.

3Biocatalysis and Biosensing Research Group, Department of Food Science and Technology, BOKU-University of Natural Resources and Life Sciences, Muthgasse 18, 1190 Vienna, Austria.

Background: Lytic polysaccharide monooxygenases (LPMOs) are copper-dependent redox enzymes that cleave recalcitrant biopolymers such as cellulose, chitin, starch and hemicelluloses. Although LPMOs receive ample interest in industry and academia, their reaction mechanism is not yet fully understood. Recent studies showed that HO is a more efficient cosubstrate for the enzyme than O, which could greatly affect the utilization of LPMOs in industrial settings. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13068-020-01673-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7057652PMC
March 2020
6.044 Impact Factor