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    1559 results match your criteria Biotechnology Advances [Journal]

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    Biotechnological potential of novel glycoside hydrolase family 70 enzymes synthesizing α-glucans from starch and sucrose.
    Biotechnol Adv 2017 Nov 10. Epub 2017 Nov 10.
    Microbial Physiology, Groningen Biomolecular Sciences and Biotechnology Institute (GBB), University of Groningen, Nijenborgh 7, 9747 AG Groningen, The Netherlands. Electronic address:
    Transglucosidases belonging to the glycoside hydrolase (GH) family 70 are promising enzymatic tools for the synthesis of α-glucans with defined structures from renewable sucrose and starch substrates. Depending on the GH70 enzyme specificity, α-glucans with different structures and physicochemical properties are produced, which have found diverse (potential) commercial applications, e.g. Read More

    Targeting ncRNAs by plant secondary metabolites: The ncRNAs game in the balance towards malignancy inhibition.
    Biotechnol Adv 2017 Nov 9. Epub 2017 Nov 9.
    MEDFUTURE - Research Center for Advanced Medicine, "Iuliu Hatieganu" University of Medicine and Pharmacy, 23 Marinescu Street, 400337, Romania; Research Center for Functional Genomics, Biomedicine and Translational Medicine, "Iuliu Hatieganu" University of Medicine and Pharmacy, 23 Marinescu Street, 400337, Cluj-Napoca, Romania; Department of Functional Genomics and Experimental Pathology, The Oncology Institute "Prof. Dr. Ion Chiricuta", Republicii 34 Street, 400015, Cluj-Napoca, Romania. Electronic address:
    The current trend of combining state of the art technologies with quondam treatments in order to overcome existing gaps in clinics determined an increased interest into polyphenols, common dietary phytochemicals, for the prevention and treatment of chronic diseases, especially cancer. The reemergence of polyphenols in the cancer field is sustained by transcriptomics technologies able to identify coding and non-coding genes and their related signaling pathways modulated by natural compounds. Identification of the structural correspondence between interacting molecules will allow the development of more targeted and informed therapeutic strategies for cancer management. Read More

    Engineering strategies for enhanced production of protein and bio-products in Pichia pastoris: A review.
    Biotechnol Adv 2017 Nov 9. Epub 2017 Nov 9.
    School of Chemical Engineering and Technology, Qingdao University of Science and Technology, Qingdao, China; Department of Chemical and Biological Engineering, University of Ottawa, 161 Louis Pasteur Private, Ottawa, ON K1N 6N5, Canada. Electronic address:
    Pichia pastoris has been recognized as one of the most industrially important hosts for heterologous protein production. Despite its high protein productivity, the optimization of P. pastoris cultivation is still imperative due to strain- and product-specific challenges such as promoter strength, methanol utilization type and oxygen demand. Read More

    Amino acid misincorporation in recombinant proteins.
    Biotechnol Adv 2017 Oct 26. Epub 2017 Oct 26.
    Process Development, Amgen Inc., 1 Amgen Center Drive, Thousand Oaks, CA 91320, United States. Electronic address:
    Proteins provide the molecular basis for cellular structure, catalytic activity, signal transduction, and molecular transport in biological systems. Recombinant protein expression is widely used to prepare and manufacture novel proteins that serve as the foundation of many biopharmaceutical products. However, protein translation bioprocesses are inherently prone to low-level errors. Read More

    Next generation organoids for biomedical research and applications.
    Biotechnol Adv 2017 Oct 19. Epub 2017 Oct 19.
    Yale Stem Cell Center, Department of Genetics, Yale School of Medicine, Yale University, New Haven, CT, United States. Electronic address:
    Organoids are in vitro cultures of miniature fetal or adult organ-like structures. Their potentials for use in tissue and organ replacement, disease modeling, toxicology studies, and drug discovery are tremendous. Currently, major challenges facing human organoid technology include (i) improving the range of cellular heterogeneity for a particular organoid system, (ii) mimicking the native micro- and matrix-environment encountered by cells within organoids, and (iii) developing robust protocols for the in vitro maturation of organoids that remain mostly fetal-like in cultures. Read More

    Potential and limitations of Klebsiella pneumoniae as a microbial cell factory utilizing glycerol as the carbon source.
    Biotechnol Adv 2017 Oct 19. Epub 2017 Oct 19.
    School of Energy and Chemical Engineering, UNIST, 50 UNIST-gil, Ulsan 44919, Republic of Korea. Electronic address:
    Klebsiella pneumoniae is a Gram-negative facultative anaerobe that metabolizes glycerol efficiently under both aerobic and anaerobic conditions. This microbe is considered an outstanding biocatalyst for transforming glycerol into a variety of value-added products. Crude glycerol is a cheap carbon source and can be converted by K. Read More

    Improved strategies for electrochemical 1,4-NAD(P)H2 regeneration: A new era of bioreactors for industrial biocatalysis.
    Biotechnol Adv 2017 Oct 10. Epub 2017 Oct 10.
    Department of Chemical and Biological Engineering, Rensselaer Polytechnic Institute, 110 8th Street, Troy, NY 12180, United States; Department of Biological Sciences, Rensselaer Polytechnic Institute, 110 8th Street, Troy, NY 12180, United States. Electronic address:
    Industrial enzymatic reactions requiring 1,4-NAD(P)H2 to perform redox transformations often require convoluted coupled enzyme regeneration systems to regenerate 1,4-NAD(P)H2 from NAD(P) and recycle the cofactor for as many turnovers as possible. Renewed interest in recycling the cofactor via electrochemical means is motivated by the low cost of performing electrochemical reactions, easy monitoring of the reaction progress, and straightforward product recovery. However, electrochemical cofactor regeneration methods invariably produce adventitious reduced cofactor side products which result in unproductive loss of input NAD(P). Read More

    Recent advances in polysaccharide bio-based flocculants.
    Biotechnol Adv 2017 Oct 7. Epub 2017 Oct 7.
    Department of Chemical Engineering and Applied Chemistry, University of Toronto, 200 College St., Toronto, Ontario M5S 3E5, Canada. Electronic address:
    Natural polysaccharides, derived from biomass feedstocks, marine resources, and microorganisms, have been attracting considerable attention as benign and environmentally friendly substitutes for synthetic polymeric products. Besides many other applications, these biopolymers are rapidly emerging as viable alternatives to harmful synthetic flocculating agents for the removal of contaminants from water and wastewater. In recent years, a great deal of effort has been devoted to improve the production and performance of polysaccharide bio-based flocculants. Read More

    Silk fibroin/hydroxyapatite composites for bone tissue engineering.
    Biotechnol Adv 2017 Oct 7. Epub 2017 Oct 7.
    Department of Biomedical Engineering, Tufts University, 4 Colby St, Medford, MA 02155, USA.
    Silk fibroin (SF) is a natural fibrous polymer with strong potential for many biomedical applications. SF has attracted interest in the field of bone tissue engineering due to its extraordinary characteristics in terms of elasticity, flexibility, biocompatibility and biodegradability. However, low osteogenic capacity has limited applications for SF in the orthopedic arena unless suitably functionalized. Read More

    Heterotrophic cultivation of microalgae for pigment production: A review.
    Biotechnol Adv 2017 Sep 22. Epub 2017 Sep 22.
    Collaborative Innovation Center of Biomass Energy, Henan Agricultural University, Henan Province, Zhengzhou 450002, China; Department of Chemical Engineering, National Taiwan University, Taipei 10617, Taiwan; Department of Chemical Engineering, National Taiwan University of Science and Technology, Taipei 10607, Taiwan. Electronic address:
    Pigments (mainly carotenoids) are important nutraceuticals known for their potent anti-oxidant activities and have been used extensively as high end health supplements. Microalgae are the most promising sources of natural carotenoids and are devoid of the toxic effects associated with synthetic derivatives. Compared to photoautotrophic cultivation, heterotrophic cultivation of microalgae in well-controlled bioreactors for pigments production has attracted much attention for commercial applications due to overcoming the difficulties associated with the supply of CO2 and light, as well as avoiding the contamination problems and land requirements in open autotrophic culture systems. Read More

    Cofactor engineering for more efficient production of chemicals and biofuels.
    Biotechnol Adv 2017 Sep 20. Epub 2017 Sep 20.
    National Energy R&D Center for Biorefinery, Beijing Key Laboratory of Bioprocess, Beijing University of Chemical Technology, 15th, Beisanhuan East Road, Beijing 100029, PR China. Electronic address:
    Cofactors are involved in numerous intracellular reactions and critically influence redox balance and cellular metabolism. Cofactor engineering can support and promote the biocatalysis process, even help driving thermodynamically unfavorable reactions forwards. To achieve efficient production of chemicals and biofuels, cofactor engineering strategies such as altering cofactor supply or modifying reactants' cofactor preference have been developed to maintain redox balance. Read More

    Current advances of succinate biosynthesis in metabolically engineered Escherichia coli.
    Biotechnol Adv 2017 Sep 20. Epub 2017 Sep 20.
    Key Laboratory of Fermentation Engineering, Ministry of Education, Hubei Provincial Cooperative Innovation Center of Industrial Fermentation, Hubei Key Laboratory of Industrial Microbiology, Hubei University of Technology, Wuhan 430068, China. Electronic address:
    As an important intermediate feedstock, succinate is termed as one of the 12 bio-based platform chemicals. To improve its fermentative production, various strategies have been developed, but challenges are still ahead for succinate biosynthesis to be cost-competitive. In this article, strategies for succinate production through metabolic engineering of Escherichia coli are critically reviewed, with a focus on engineering by-product formation and CO2 fixation, substrate utilization, reducing power balance, metabolic evolution and transcriptional regulation, which provide insights for the current state of succinate production and perspectives for further research for more efficient and economical production of bio-based succinate. Read More

    Learning from quantitative data to understand central carbon metabolism.
    Biotechnol Adv 2017 Sep 18. Epub 2017 Sep 18.
    Department of Bioinformatic Engineering, Graduate School of Information Science and Technology, Osaka University, 1-5 Yamadaoka, Suita, Osaka 565-0871, Japan. Electronic address:
    Quantitative analysis of metabolism has been used to identify driver reactions occurring during cancer development and bottleneck reactions in the metabolic engineering of microorganisms. In this review, we compare the advantages and disadvantages of various metabolic analysis methods. We emphasize that metabolic flux analysis based on material balance is a critical method for quantitative investigations into cell metabolism. Read More

    Formulation, construction and analysis of kinetic models of metabolism: A review of modelling frameworks.
    Biotechnol Adv 2017 Sep 13. Epub 2017 Sep 13.
    Australian Institute for Bioengineering and Nanotechnology (AIBN), The University of Queensland, Brisbane, QLD 4072, Australia; The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kemitorvet, Building 220, DK-2800 Kongens Lyngby, Denmark. Electronic address:
    Kinetic models are critical to predict the dynamic behaviour of metabolic networks. Mechanistic kinetic models for large networks remain uncommon due to the difficulty of fitting their parameters. Recent modelling frameworks promise new ways to overcome this obstacle while retaining predictive capabilities. Read More

    Zika virus structural biology and progress in vaccine development.
    Biotechnol Adv 2017 Sep 12. Epub 2017 Sep 12.
    Institute of Biotechnology, National Tsing Hua University, Hsinchu, Taiwan; Department of Medical Science, National Tsing Hua University, Hsinchu, Taiwan. Electronic address:
    The growing number of zika virus (ZIKV) infections plus a 20-fold increase in neonatal microcephaly in newborns in Brazil have raised alarms in many countries regarding the threat to pregnant women. Instances of microcephaly and central nervous system malformations continue to increase in ZIKV outbreak regions. ZIKV is a small enveloped positive-strand RNA virus belonging to the Flavivirus genus of the Flaviviridae family. Read More

    Taxonomy, ecology and biotechnological applications of thraustochytrids: A review.
    Biotechnol Adv 2017 Sep 11. Epub 2017 Sep 11.
    Institute of Mechanical, Process & Energy Engineering, School of Engineering and Physical Sciences, Heriot-Watt University, Edinburgh EH14 4AS, UK. Electronic address:
    Thraustochytrids were first discovered in 1934, and since the 1960's they have been increasingly studied for their beneficial and deleterious effects. This review aims to provide an enhanced understanding of these protists with a particular emphasis on their taxonomy, ecology and biotechnology applications. Over the years, thraustochytrid taxonomy has improved with the development of modern molecular techniques and new biochemical markers, resulting in the isolation and description of new strains. Read More

    Chassis and key enzymes engineering for monoterpenes production.
    Biotechnol Adv 2017 Sep 6. Epub 2017 Sep 6.
    Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, PR China; SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin University, Tianjin 300072, PR China. Electronic address:
    Microbial production of monoterpenes is often limited by their cytotoxicity and in vivo conversion. Therefore, alleviating cytotoxicity and reducing conversion by chassis engineering are highly desirable. On the other hand, engineering key enzymes is also critical for improving monoterpenes production through facilitating the biosynthesis process. Read More

    Amino acids production focusing on fermentation technologies - A review.
    Biotechnol Adv 2017 Sep 6. Epub 2017 Sep 6.
    Technical University of Denmark, Department of Environmental Engineering, Building 113, DK-2800 Kgs. Lyngby, Denmark. Electronic address:
    Amino acids are attractive and promising biochemicals with market capacity requirements constantly increasing. Their applicability ranges from animal feed additives, flavour enhancers and ingredients in cosmetic to specialty nutrients in pharmaceutical and medical fields. This review gives an overview of the processes applied for amino acids production and points out the main advantages and disadvantages of each. Read More

    Growth media in anaerobic fermentative processes: The underestimated potential of thermophilic fermentation and anaerobic digestion.
    Biotechnol Adv 2017 Sep 1. Epub 2017 Sep 1.
    Section Sanitary Engineering, Department of Water Management, Delft University of Technology, Stevinweg 1, 2628CN Delft, The Netherlands.
    Fermentation and anaerobic digestion of organic waste and wastewater is broadly studied and applied. Despite widely available results and data for these processes, comparison of the generated results in literature is difficult. Not only due to the used variety of process conditions, but also because of the many different growth media that are used. Read More

    In-vitro in-vivo correlation (IVIVC) in nanomedicine: Is protein corona the missing link?
    Biotechnol Adv 2017 Nov 26;35(7):889-904. Epub 2017 Aug 26.
    Faculty of Pharmacy, VNS Group of Institutions, Nathu Barkheda, Neelbud, Bhopal 462044, India. Electronic address:
    One of the unmet challenges in nanotechnology is to understand and establish the relationship between physicochemical properties of nanoparticles (NPs) and its biological interactions (bio-nano interactions). However, we are still far from assessing the biofate of NPs in a clear and unquestionable manner. Recent developments in the area of bio-nano interface and the understanding of protein corona (PC) has brought new insight in predicting biological interactions of NPs. Read More

    Covalent and non-covalent chemical engineering of actin for biotechnological applications.
    Biotechnol Adv 2017 Nov 19;35(7):867-888. Epub 2017 Aug 19.
    Department of Chemistry and Biomedical Sciences, Faculty of Health and Life Sciences, Linnaeus University, SE-391 82 Kalmar, Sweden. Electronic address:
    The cytoskeletal filaments are self-assembled protein polymers with 8-25nm diameters and up to several tens of micrometres length. They have a range of pivotal roles in eukaryotic cells, including transportation of intracellular cargoes (primarily microtubules with dynein and kinesin motors) and cell motility (primarily actin and myosin) where muscle contraction is one example. For two decades, the cytoskeletal filaments and their associated motor systems have been explored for nanotechnological applications including miniaturized sensor systems and lab-on-a-chip devices. Read More

    Bioremediation 3.0: Engineering pollutant-removing bacteria in the times of systemic biology.
    Biotechnol Adv 2017 Nov 5;35(7):845-866. Epub 2017 Aug 5.
    Systems and Synthetic Biology Program, Centro Nacional de Biotecnología (CNB-CSIC), 28049 Madrid, Spain. Electronic address:
    Elimination or mitigation of the toxic effects of chemical waste released to the environment by industrial and urban activities relies largely on the catalytic activities of microorganisms-specifically bacteria. Given their capacity to evolve rapidly, they have the biochemical power to tackle a large number of molecules mobilized from their geological repositories through human action (e.g. Read More

    Breaking the mirror: l-Amino acid deaminase, a novel stereoselective biocatalyst.
    Biotechnol Adv 2017 Nov 3;35(6):657-668. Epub 2017 Aug 3.
    Dipartimento di Biotecnologie e Scienze della Vita, Università degli Studi deII'Insubria, via J. H. Dunant 3, 21100 Varese, Italy; The Protein Factory, Centro Interuniversitario di Biotecnologie Proteiche, Politecnico di Milano and Università degli Studi dell'Insubria, Varese, Italy. Electronic address:
    Enantiomerically pure amino acids are of increasing interest for the fine chemical, agrochemicals and pharmaceutical industries. During past years l-amino acids have been produced from deracemization of dl-solution employing the stereoselective flavoenzyme d-amino acid oxidase. On the other hand, the isolation of corresponding d-isomer was hampered by the scarce availability of a suitable l-amino acid oxidase activity. Read More

    Weaving for heart valve tissue engineering.
    Biotechnol Adv 2017 Nov 4;35(6):633-656. Epub 2017 Aug 4.
    Aesculap AG, Carl-Braun-Straße 1, 34212 Melsungen, Germany.
    Weaving is a resourceful technology which offers a large selection of solutions that are readily adaptable for tissue engineering (TE) of artificial heart valves (HV). The different ways that the yarns are interlaced in this technique could be used to produce complex architectures, such as the three-layer architecture of the leaflets. Once the assembly is complete, growth of cells in the scaffold would occur in the orientation of the yarn, enabling the deposition of extra cellular matrixes proteins in an oriented manner. Read More

    Stay connected: Electrical conductivity of microbial aggregates.
    Biotechnol Adv 2017 Nov 30;35(6):669-680. Epub 2017 Jul 30.
    Biological and Ecological Engineering, Oregon State University, Corvallis, OR 97333, USA. Electronic address:
    The discovery of direct extracellular electron transfer offers an alternative to the traditional understanding of diffusional electron exchange via small molecules. The establishment of electronic connections between electron donors and acceptors in microbial communities is critical to electron transfer via electrical currents. These connections are facilitated through conductivity associated with various microbial aggregates. Read More

    Towards systems metabolic engineering in Pichia pastoris.
    Biotechnol Adv 2017 Nov 29;35(6):681-710. Epub 2017 Jul 29.
    Fermentation Engineering, Bielefeld University, Universitätsstr. 25, Bielefeld 33615, Germany. Electronic address:
    The methylotrophic yeast Pichia pastoris is firmly established as a host for the production of recombinant proteins, frequently outperforming other heterologous hosts. Already, a sizeable amount of systems biology knowledge has been acquired for this non-conventional yeast. By applying various omics-technologies, productivity features have been thoroughly analyzed and optimized via genetic engineering. Read More

    Glycosaminoglycans from marine sources as therapeutic agents.
    Biotechnol Adv 2017 Nov 21;35(6):711-725. Epub 2017 Jul 21.
    Group of Recycling and Valorisation of Waste Materials (REVAL), Marine Research Institute (IIM-CSIC), R/Eduardo Cabello, 6, CP 36208, Vigo, Pontevedra, Spain. Electronic address:
    Glycosaminoglycans (GAGs) in marine animals are different to those of terrestrial organisms, mainly in terms of molecular weight and sulfation. The therapeutic properties of GAGs are related to their ability to interact with proteins, which is very much influenced by sulfation position and patterns. Since currently GAGs cannot be chemically synthesized, they are sourced from natural products, with high intra- but also inter-species variability, in terms of chain length, disaccharide composition and sulfation pattern. Read More

    Valorisation of softwood bark through extraction of utilizable chemicals. A review.
    Biotechnol Adv 2017 Nov 21;35(6):726-750. Epub 2017 Jul 21.
    Institute of Natural and Synthetic Polymers, Department of Wood, Pulp and Paper, Faculty of Chemical and Food Technology, Slovak University of Technology in Bratislava, Radlinského 9, Bratislava SK-812 37, Slovakia.
    Softwood bark is an important source for producing chemicals and materials as well as bioenergy. Extraction is regarded as a key technology for obtaining chemicals in general, and valorizing bark as a source of such chemicals in particular. In this paper, properties of 237 compounds identified in various studies dealing with extraction of softwood bark were described. Read More

    Insight into immunocytes infiltrations in polymorphous light eruption.
    Biotechnol Adv 2017 Nov 17;35(6):751-757. Epub 2017 Jul 17.
    Department of Dermatology, First Affiliated Hospital of Kunming Medical University, Institute of Dermatology & Venereology of Yunnan Province, Kunming, Yunnan, China. Electronic address:
    Polymorphous light eruption (PLE) which is one of the most common photodermatoses has been demonstrated to be immune-mediated disorder. Resistance to UV-induced immunosuppression resulting from differential immune cells infiltration and cytokines secretion has been highlighted in the pathogenesis of PLE. In this study, we reviewed differential patterns of immune cells infiltrations and cytokines secretion that may contribute to PLE occurrence and development. Read More

    Enabling tools for high-throughput detection of metabolites: Metabolic engineering and directed evolution applications.
    Biotechnol Adv 2017 Jul 16. Epub 2017 Jul 16.
    McKetta Department of Chemical Engineering, The University of Texas at Austin, 200 E Dean Keeton St. Stop C0400, Austin, TX 78712, United States; Institute for Cellular and Molecular Biology, The University of Texas at Austin, 2500 Speedway Avenue, Austin, TX 78712, United States. Electronic address:
    Within the Design-Build-Test Cycle for strain engineering, rapid product detection and selection strategies remain challenging and limit overall throughput. Here we summarize a wide variety of modalities that transduce chemical concentrations into easily measured absorbance, luminescence, and fluorescence signals. Specifically, we cover protein-based biosensors (including transcription factors), nucleic acid-based biosensors, coupled enzyme reactions, bioorthogonal chemistry, and fluorescent and chromogenic dyes and substrates as modalities for detection. Read More

    Bioelectrohydrogenesis and inhibition of methanogenic activity in microbial electrolysis cells - A review.
    Biotechnol Adv 2017 Nov 12;35(6):758-771. Epub 2017 Jul 12.
    Department of Biology and Sino-Forest Applied Research Centre for Pearl River Delta Environment, Hong Kong Baptist University, Hong Kong, Hong Kong Special Administrative Region. P.R. China. Electronic address:
    Microbial electrolysis cells (MECs) are a promising technology for biological hydrogen production. Compared to abiotic water electrolysis, a much lower electrical voltage (~0.2V) is required for hydrogen production in MECs. Read More

    Microalgal-bacterial aggregates: Applications and perspectives for wastewater treatment.
    Biotechnol Adv 2017 Nov 8;35(6):772-781. Epub 2017 Jul 8.
    Laboratory for Research on Advanced Processes for Water Treatment, Instituto de Ingeniería, Unidad Académica Juriquilla, Universidad Nacional Autónoma de México, Blvd. Juriquilla 3001, Querétaro 76230, Mexico. Electronic address:
    Research on wastewater treatment by means of microalgal-bacterial processes has become a hot topic worldwide during the last two decades. Owing to the lower energy demand for oxygenation, the enhanced nutrient removal and the potential for resource recovery, microalgal-based technologies are nowadays considered as a good alternative to conventional activated sludge treatments in many instances. Nevertheless, biomass harvesting still constitutes one of the major challenges of microalgal-bacterial systems for wastewater treatment, which is hindered by the poor settleability of microalgal biomass. Read More

    Medicinal plants used in the treatment of tuberculosis - Ethnobotanical and ethnopharmacological approaches.
    Biotechnol Adv 2017 Jul 8. Epub 2017 Jul 8.
    Department of Agricultural and Environmental Sciences, Milan State University, via G. Celoria 2, Milan 20133, Italy. Electronic address:
    Tuberculosis is a highly infectious disease declared a global health emergency by the World Health Organization, with approximately one third of the world's population being latently infected with Mycobacterium tuberculosis. Tuberculosis treatment consists in an intensive phase and a continuation phase. Unfortunately, the appearance of multi drug-resistant tuberculosis, mainly due to low adherence to prescribed therapies or inefficient healthcare structures, requires at least 20months of treatment with second-line, more toxic and less efficient drugs, i. Read More

    Spheroids as vascularization units: From angiogenesis research to tissue engineering applications.
    Biotechnol Adv 2017 Nov 8;35(6):782-791. Epub 2017 Jul 8.
    Institute for Clinical & Experimental Surgery, Saarland University, 66421 Homburg/Saar, Germany.
    Multi-cellular spheroids mimic the complex three-dimensional environment of natural tissues. Accordingly, they are also used as vascularization units in angiogenesis research and regenerative medicine. Spheroid sprouting assays are versatile in vitro models for the analysis of molecular and cellular determinants of blood vessel development, including different endothelial cell phenotypes, pro- and anti-angiogenic factors as well as cell-matrix and cell-cell interactions. Read More

    GH62 arabinofuranosidases: Structure, function and applications.
    Biotechnol Adv 2017 Nov 29;35(6):792-804. Epub 2017 Jun 29.
    Enzyme and Protein Chemistry, Department of Biotechnology and Biomedicine, Technical University of Denmark, Elektrovej, Building 375, DK-2800 Kgs. Lyngby, Denmark. Electronic address:
    Motivated by industrial demands and ongoing scientific discoveries continuous efforts are made to identify and create improved biocatalysts dedicated to plant biomass conversion. α-1,2 and α-1,3 arabinofuranosyl specific α-l-arabinofuranosidases (EC 3.2. Read More

    Channeling in native microbial pathways: Implications and challenges for metabolic engineering.
    Biotechnol Adv 2017 Nov 13;35(6):805-814. Epub 2017 Jun 13.
    Department of Energy, Environmental and Chemical Engineering, Washington University, St. Louis, MO 63130, USA. Electronic address:
    Intracellular enzymes can be organized into a variety of assemblies, shuttling intermediates from one active site to the next. Eukaryotic compartmentalization within mitochondria and peroxisomes and substrate tunneling within multi-enzyme complexes have been well recognized. Intriguingly, the central pathways in prokaryotes may also form extensive channels, including the heavily branched glycolysis pathway. Read More

    Oxidoreductases on their way to industrial biotransformations.
    Biotechnol Adv 2017 Nov 15;35(6):815-831. Epub 2017 Jun 15.
    Instituto de Catálisis y Petroleoquímica, CSIC, Madrid, Spain.
    Fungi produce heme-containing peroxidases and peroxygenases, flavin-containing oxidases and dehydrogenases, and different copper-containing oxidoreductases involved in the biodegradation of lignin and other recalcitrant compounds. Heme peroxidases comprise the classical ligninolytic peroxidases and the new dye-decolorizing peroxidases, while heme peroxygenases belong to a still largely unexplored superfamily of heme-thiolate proteins. Nevertheless, basidiomycete unspecific peroxygenases have the highest biotechnological interest due to their ability to catalyze a variety of regio- and stereo-selective monooxygenation reactions with H2O2 as the source of oxygen and final electron acceptor. Read More

    Progress and biotechnological prospects in fish transgenesis.
    Biotechnol Adv 2017 Nov 8;35(6):832-844. Epub 2017 Jun 8.
    Laboratório de Sinalização Celular e Nanobiotecnologia, Departamento de Bioquímica e Imunologia, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil; Instituto Nanocell, Divinópolis, MG, Brazil. Electronic address:
    The history of transgenesis is marked by milestones such as the development of cellular transdifferentiation, recombinant DNA, genetic modification of target cells, and finally, the generation of simpler genetically modified organisms (e.g. bacteria and mice). Read More

    Current advances on fermentative biobutanol production using third generation feedstock.
    Biotechnol Adv 2017 Jun 1. Epub 2017 Jun 1.
    State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin, China; Department of Chemical Engineering, National Cheng Kung University, Tainan, Taiwan; Research Center for Energy Technology and Strategy, National Cheng Kung University, Tainan 701, Taiwan. Electronic address:
    Biobutanol is gaining more attention as a potential alternative to ethanol, and the demand for fermentative biobutanol production has renewed interest. The main challenge faced in biobutanol production is the availability of feedstock. Using conventional agricultural biomass as feedstock is controversial and less efficient, while microalgae, the third generation feedstock, are considered promising feedstock for biobutanol production due to their high growth rate and high carbohydrates content. Read More

    Biocompatibility of hydrogel-based scaffolds for tissue engineering applications.
    Biotechnol Adv 2017 Sep 27;35(5):530-544. Epub 2017 May 27.
    Department of Chemical Engineering, University of Waterloo, 200 University Avenue West, Waterloo, Ontario N2L 3G1, Canada; Waterloo Institute for Nanotechnology, University of Waterloo, Ontario, Canada. Electronic address:
    Recently, understanding of the extracellular matrix (ECM) has expanded rapidly due to the accessibility of cellular and molecular techniques and the growing potential and value for hydrogels in tissue engineering. The fabrication of hydrogel-based cellular scaffolds for the generation of bioengineered tissues has been based on knowledge of the composition and structure of ECM. Attempts at recreating ECM have used either naturally-derived ECM components or synthetic polymers with structural integrity derived from hydrogels. Read More

    Three-dimensional printing: technologies, applications, and limitations in neurosurgery.
    Biotechnol Adv 2017 Sep 24;35(5):521-529. Epub 2017 May 24.
    Columbia University Medical Center Department of Neurological Surgery, 710 W 168th Street, New York, NY 10032, United States. Electronic address:
    Three-dimensional (3D) printers are a developing technology penetrating a variety of markets, including the medical sector. Since its introduction to the medical field in the late 1980s, 3D printers have constructed a range of devices, such as dentures, hearing aids, and prosthetics. With the ultimate goals of decreasing healthcare costs and improving patient care and outcomes, neurosurgeons are utilizing this dynamic technology, as well. Read More

    Molecular features of grass allergens and development of biotechnological approaches for allergy prevention.
    Biotechnol Adv 2017 Sep 20;35(5):545-556. Epub 2017 May 20.
    Joint International Research Laboratory of Metabolic & Developmental Sciences, University of Adelaide-Shanghai Jiao Tong University Joint Centre for Agriculture and Health, School of Agriculture, Food and Wine, University of Adelaide, Waite Campus, Urrbrae, South Australia 5064, Australia; School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, China. Electronic address:
    Allergic diseases are characterized by elevated allergen-specific IgE and excessive inflammatory cell responses. Among the reported plant allergens, grass pollen and grain allergens, derived from agriculturally important members of the Poaceae family such as rice, wheat and barley, are the most dominant and difficult to prevent. Although many allergen homologs have been predicted from species such as wheat and timothy grass, fundamental aspects such as the evolution and function of plant pollen allergens remain largely unclear. Read More

    Ubiquitous Chromatin-opening Elements (UCOEs): Applications in biomanufacturing and gene therapy.
    Biotechnol Adv 2017 Sep 17;35(5):557-564. Epub 2017 May 17.
    Gene Expression and Therapy Group, King's College London, Faculty of Life Sciences and Medicine, Department of Molecular Genetics, Guy's Hospital, London Bridge, London SE1 9RT, United Kingdom. Electronic address:
    Ubiquitous Chromatin-opening Elements (UCOEs) are defined by their ability to consistently confer stable, site of integration-independent transgene expression that is proportional to copy number, including from within regions of heterochromatin such as centromeres. UCOEs structurally consist of methylation-free CpG islands encompassing single or dual divergently-transcribed housekeeping gene promoters. Since their discovery in 1999, UCOEs and their sub-fragments have found applications in areas of biotechnology requiring stable, reproducible, and high levels of gene expression. Read More

    Harnessing self-assembled peptide nanoparticles in epitope vaccine design.
    Biotechnol Adv 2017 Sep 15;35(5):575-596. Epub 2017 May 15.
    Department of Pharmaceutical Biotechnology, School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran; Pharmaceutical Sciences Research Center, Shiraz University of Medical Sciences, Shiraz, Iran; Department of Medical Biotechnology, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran; Biotechnology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran. Electronic address:
    Vaccination has been one of the most successful breakthroughs in medical history. In recent years, epitope-based subunit vaccines have been introduced as a safer alternative to traditional vaccines. However, they suffer from limited immunogenicity. Read More

    Bioengineering bacterial outer membrane vesicles as vaccine platform.
    Biotechnol Adv 2017 Sep 15;35(5):565-574. Epub 2017 May 15.
    Institute for Translational Vaccinology (Intravacc), Process Development Bacterial Vaccines, P.O. Box 450, 3720 AL Bilthoven, The Netherlands. Electronic address:
    Outer membrane vesicles (OMVs) are naturally non-replicating, highly immunogenic spherical nanoparticles derived from Gram-negative bacteria. OMVs from pathogenic bacteria have been successfully used as vaccines against bacterial meningitis and sepsis among others and the composition of the vesicles can easily be engineered. OMVs can be used as a vaccine platform by engineering heterologous antigens to the vesicles. Read More

    Occurrence and biosynthesis of carotenoids in phytoplankton.
    Biotechnol Adv 2017 Sep 13;35(5):597-618. Epub 2017 May 13.
    Food and Nutritional Sciences Programme, School of Life Sciences, The Chinese University of Hong Kong, Shatin, Hong Kong S.A.R., People's Republic of China. Electronic address:
    Naturally occurring carotenoids are important sources of antioxidants, anti-cancer compounds and anti-inflammatory agents and there is thus considerable market demand for their pharmaceutical applications. Carotenoids are widely distributed in marine and freshwater organisms including microalgae, phytoplankton, crustaceans and fish, as well as in terrestrial plants and birds. Recently, phytoplankton-derived carotenoids have received much attention due to their abundance, rapid rate of biosynthesis and unique composition. Read More

    Maltooligosaccharide-forming amylase: Characteristics, preparation, and application.
    Biotechnol Adv 2017 Sep 27;35(5):619-632. Epub 2017 Apr 27.
    School of Food Science and Technology, Jiangnan University, Wuxi 214122, People's Republic of China; State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, People's Republic of China; Collaborative Innovation Center for Food Safety and Quality Control, Jiangnan University, Wuxi 214122, People's Republic of China. Electronic address:
    As member of glycosyl hydrolase family 13, maltooligosaccharide-forming amylases (MFAses) are specific and interesting because of their capacity to hydrolyze starch into functional maltooligosaccharides, which are usually composed of 2-10 α-d-glucopyranosyl units linked by α-1,4 glycosidic linkages. MFAses have been extensively studied during recent decades, and have shown promise in various industrial applications. This review begins by introducing the potential uses of maltooligosaccharides. Read More

    Transcriptomics of human multipotent mesenchymal stromal cells: Retrospective analysis and future prospects.
    Biotechnol Adv 2017 Jul 24;35(4):407-418. Epub 2017 Apr 24.
    Institute of Biomedical Engineering, Department of Engineering Science, University of Oxford, Oxford, United Kingdom. Electronic address:
    The plastic-adherent, fibroblast-like, clonogenic cells found in the human body now defined as multipotent "Mesenchymal Stromal Cells" (MSCs) hold immense potential for cell-based therapies. Recently, research and basic knowledge of these cells has fast-tracked, both from fundamental and translational perspectives. There have been important discoveries with respect to the available variety of tissue sources, the development of protocols for their easy isolation and in vitro expansion and for directed differentiation into various cell types. Read More

    Toward biotechnology in space: High-throughput instruments for in situ biological research beyond Earth.
    Biotechnol Adv 2017 Nov 19;35(7):905-932. Epub 2017 Apr 19.
    University of California San Francisco, Department of Pharmaceutical Chemistry, San Francisco, CA 94158, USA; NASA Ames Research Center, Exobiology Branch, MS239-4, Moffett Field, CA 94035, USA. Electronic address:
    Space biotechnology is a nascent field aimed at applying tools of modern biology to advance our goals in space exploration. These advances rely on our ability to exploit in situ high throughput techniques for amplification and sequencing DNA, and measuring levels of RNA transcripts, proteins and metabolites in a cell. These techniques, collectively known as "omics" techniques have already revolutionized terrestrial biology. Read More

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