360,032 results match your criteria Biotechnology Advances [Journal]


Implementation of a Pipeline Using Disease-Disease Associations for Computational Drug Repurposing.

Methods Mol Biol 2019 ;1903:129-148

Department of Biotechnology, School of Bio Sciences and Technology, Vellore Institute of Technology, Vellore, Tamil Nadu, India.

Drug repurposing is a powerful technique which has been recently employed in both industry and academia to discover and validate previously approved drugs for new indications. It provides the quickest possible transition from bench to bedside. In essence, computational strategies are appealing because they putatively nominate the most encouraging candidate for a given indication. Read More

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January 2019

HOOKLESS1 is a positive regulator in Arabidopsis thermomorphogenesis.

Sci China Life Sci 2018 Dec 11. Epub 2018 Dec 11.

Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing, 210023, China.

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December 2018

Spatial and temporal heterogeneity in the structure and function of sediment bacterial communities of a tropical mangrove forest.

Environ Sci Pollut Res Int 2018 Dec 13. Epub 2018 Dec 13.

Wetland Research and Training Centre, Chilika Development Authority, Balugaon, Odisha, 752030, India.

Bacterial communities of mangrove sediments are well appreciated for their role in nutrient cycling. However, spatiotemporal variability in these communities over large geographical scale remains understudied. We investigated sediment bacterial communities and their metabolic potential in an intertidal mangrove forest of India, Bhitarkanika, using high-throughput sequencing of 16S rRNA genes and community-level physiological profiling. Read More

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December 2018

Early Hereditary Diffuse Gastric Cancer (eHDGC) is Characterized by Subtle Genomic Instability and Active DNA Damage Response.

Pathol Oncol Res 2018 Dec 13. Epub 2018 Dec 13.

Division of Biomedical Science and Biochemistry, Research School of Biology, ANU College of Medicine, Biology and Environment, Australian National University, P.O. Box: 2601, Canberra, Australia.

Diffuse gastric cancer (DGC) is one of the two primary types of stomach cancer. Carriers of germline mutations in the gene encoding E-cadherin are predisposed to DGC. The primary aim of the present study was to determine if genomic instability is an early event in DGC and how it may lead to disease progression. Read More

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December 2018
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Chemical transformation mediated CRISPR/Cas9 genome editing in Escherichia coli.

Biotechnol Lett 2018 Dec 13. Epub 2018 Dec 13.

College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, 310014, Zhejiang, People's Republic of China.

Objectives: To develop a convenient chemical transformation mediated CRISPR/Cas9 (CT-CRISPR/Cas9) system for genome editing in Escherichia coli.

Results: Here, we have constructed a CT-CRISPR/Cas9 system, which can precisely edit bacterial genome (replacing, deleting, inserting or point mutating a target gene) through chemical transformation. Compared with the traditional electroporation mediated CRISPR/Cas9 (ET-CRISPR/Cas9) system, genome editing with the CT-CRISPR/Cas9 system is much cheaper and simpler. Read More

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December 2018

Increases in cytosolic Ca induce dynamin- and calcineurin-dependent internalisation of CFTR.

Cell Mol Life Sci 2018 Dec 13. Epub 2018 Dec 13.

Institute for Cell and Molecular Biosciences, Newcastle University, Newcastle upon Tyne, NE2 4HH, UK.

The cystic fibrosis transmembrane conductance regulator (CFTR) is a cAMP-regulated, apical anion channel that regulates ion and fluid transport in many epithelia including the airways. We have previously shown that cigarette smoke (CS) exposure to airway epithelia causes a reduction in plasma membrane CFTR expression which correlated with a decrease in airway surface hydration. The effect of CS on CFTR was dependent on an increase in cytosolic Ca. Read More

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December 2018
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Breaking the curse of dimensionality to identify causal variants in Breeding 4.

Theor Appl Genet 2018 Dec 13. Epub 2018 Dec 13.

Institute for Genomic Diversity, Institute of Biotechnology, Cornell University, 175 Biotechnology Building, Ithaca, NY, 14853, USA.

In the past, plant breeding has undergone three major transformations and is currently transitioning to a new technological phase, Breeding 4. This phase is characterized by the development of methods for biological design of plant varieties, including transformation and gene editing techniques directed toward causal loci. The application of such technologies will require to reliably estimate the effect of loci in plant genomes by avoiding the situation where the number of loci assayed (p) surpasses the number of plant genotypes (n). Read More

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December 2018
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Characterisation of barley resistance to rhynchosporium on chromosome 6HS.

Theor Appl Genet 2018 Dec 13. Epub 2018 Dec 13.

The James Hutton Institute, Invergowrie, Dundee, DD2 5DA, Scotland, UK.

Key Message: Major resistance gene to rhynchosporium, Rrs18, maps close to the telomere on the short arm of chromosome 6H in barley. Rhynchosporium or barley scald caused by a fungal pathogen Rhynchosporium commune is one of the most destructive and economically important diseases of barley in the world. Testing of Steptoe × Morex and CIho 3515 × Alexis doubled haploid populations has revealed a large effect QTL for resistance to R. Read More

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December 2018
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Long segment detection of HTLV-1 genome based on the fluorescence quenching technique.

Heliyon 2018 Dec 5;4(12):e00996. Epub 2018 Dec 5.

Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran.

Detecting fluorescence changes due to energy transfer between a quencher and fluorophore is a common method used for the fluorescence-based biosensors. In the present report, a new biosensor for long segment detection of the human T cell-lymphotropic virus 1 genome was constructed based on the fluorescence quenching of graphene oxide by gold nanoparticles. The fluorescence signal of unmodified graphene oxide was measured before and after hybridization of target and probes functionalized with gold nanoparticles. Read More

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December 2018

The nanoscaled metal-organic framework ICR-2 as a carrier of porphyrins for photodynamic therapy.

Beilstein J Nanotechnol 2018 30;9:2960-2967. Epub 2018 Nov 30.

Department of Materials Chemistry, Institute of Inorganic Chemistry of the Czech Academy of Sciences, Husinec-Řež 1001, 250 68 Řež, Czech Republic.

Nanosized porphyrin-containing metal-organic frameworks (MOFs) attract considerable attention as solid-state photosensitizers for biological applications. In this study, we have for the first time synthesised and characterised phosphinate-based MOF nanoparticles, nanoICR-2 (Inorganic Chemistry Rez). We demonstrate that nanoICR-2 can be decorated with anionic 5,10,15,20-tetrakis(4-R-phosphinatophenyl)porphyrins (R = methyl, isopropyl, phenyl) by utilizing unsaturated metal sites on the nanoparticle surface. Read More

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November 2018

BRAF and MEK inhibitors differentially affect nivolumab-induced T cell activation by modulating the TCR and AKT signaling pathways.

Oncoimmunology 2019 29;8(1):e1512456. Epub 2018 Oct 29.

Office of Biotechnology Products, Center for Drug Evaluation and Research, U.S. Food and Drug Administration, Silver Spring, MD, USA.

Immune checkpoint inhibitors (ICIs) such as the anti-PD-1 antibody Nivolumab, achieve remarkable clinical efficacy in patients with late stage cancers. However, only a small subset of patients benefit from this therapy. Numerous clinical trials are underway testing whether combining ICIs with other anti-cancer therapies can increase this response rate. Read More

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October 2018

YaTCM: Yet another Traditional Chinese Medicine Database for Drug Discovery.

Comput Struct Biotechnol J 2018 23;16:600-610. Epub 2018 Nov 23.

State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy and Tianjin Key Laboratory of Molecular Drug Research, Nankai University, Haihe Education Park, 38 Tongyan Road, Tianjin 300353, China.

Traditional Chinese Medicine (TCM) has a long history of widespread clinical applications, especially in East Asia, and is becoming frequently used in Western countries. However, owing to extreme complicacy in both chemical ingredients and mechanism of action, a deep understanding of TCM is still difficult. To accelerate the modernization and popularization of TCM, a single comprehensive database is required, containing a wealth of TCM-related information and equipped with complete analytical tools. Read More

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November 2018

Human liver stem cell-derived extracellular vesicles enhance cancer stem cell sensitivity to tyrosine kinase inhibitors through Akt/mTOR/PTEN combined modulation.

Oncotarget 2018 Nov 16;9(90):36151-36165. Epub 2018 Nov 16.

Department of Molecular Biotechnology and Health Sciences, Torino, Italy.

It is well recognized that Cancer Stem Cells (CSCs) sustain the initiation, the maintenance and the recurrence of tumors. We previously reported that extracellular vesicles (EVs) derived from human liver stem cells (HLSCs) were able to limit tumor development. In this study, we evaluated whether EV derived from HLSCs could act in synergy with tyrosine kinase inhibitors (TKIs) on apoptosis of CSCs isolated from renal carcinomas. Read More

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November 2018

Late adult-onset adrenomyeloneuropathy evolving with atypical severe frontal lobe syndrome: Importance of neuroimaging.

Radiol Case Rep 2019 Mar 5;14(3):309-314. Epub 2018 Dec 5.

Department of Medical, Surgical, Neurological, Metabolic Sciences, and Aging, 2nd Division of Neurology, Center for Rare Diseases and InterUniversity Center for Research in Neurosciences, University of Campania "Luigi Vanvitelli", Via Sergio Pansini, 80131 Naples, Italy.

X-linked adrenoleukodystrophy (X-ALD) is a rare inherited metabolic disease affecting the nervous system and the adrenal glands. It is caused by a mutation of the gene, resulting in the impaired degradation of very long-chain fatty acids and their subsequent accumulation in several organs and tissues. X-ALD is notable for its high phenotypical variability, that includes isolated adrenocortical insufficiency, slowly progressive myelopathy with paraparesis, ataxia, and peripheral neuropathy to severe childhood cerebral forms. Read More

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Transaminase-Catalyzed Racemization with Potential for Dynamic Kinetic Resolutions.

ChemCatChem 2018 Nov 11;10(21):5012-5018. Epub 2018 Oct 11.

Department of Industrial Biotechnology KTH Royal Institute of Technology AlbaNova University Center Stockholm SE-106 91 Sweden.

Dynamic kinetic resolution (DKR) reactions in which a stereoselective enzyme and a racemization step are coupled in one pot would represent powerful tools for the production of enantiopure amines through enantioconvergence of racemates. The exploitation of DKR strategies is currently hampered by the lack of effective, enzyme-compatible and scalable racemization strategies for amines. In the present work, the proof of concept of a fully biocatalytic method for amine racemization is presented. Read More

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November 2018
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Olefin metathesis catalysts embedded in β-barrel proteins: creating artificial metalloproteins for olefin metathesis.

Beilstein J Org Chem 2018 19;14:2861-2871. Epub 2018 Nov 19.

Institute of Inorganic Chemistry, RWTH Aachen University, Landoltweg 1, 52074 Aachen, Germany.

This review summarizes the recent progress of Grubbs-Hoveyda (GH) type olefin metathesis catalysts incorporated into the robust fold of β-barrel proteins. Anchoring strategies are discussed and challenges and opportunities in this emerging field are shown from simple small-molecule transformations over ring-opening metathesis polymerizations to in vivo olefin metathesis. Read More

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November 2018

ALK expression favorably impacts the prognosis of -mutated metastatic melanomas.

Oncol Lett 2018 Dec 9;16(6):7091-7096. Epub 2018 Oct 9.

Department of Molecular Biotechnology and Health Sciences, Center for Experimental Research and Medical Studies, University of Torino, I-10126 Torino, Italy.

Recent studies reported the expression of anaplastic lymphoma kinase (ALK) in malignant melanomas. The aim of this study was to investigate whether ALK expression is associated with specific clinical and molecular characteristics of melanoma metastases, and to evaluate its correlation with survival outcomes. Seventy-one patients with metastatic melanoma were investigated. Read More

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December 2018

Novel insight into the function of tankyrase.

Authors:
Mi Kyung Kim

Oncol Lett 2018 Dec 5;16(6):6895-6902. Epub 2018 Oct 5.

Department of Systems Biology, College of Life Science and Biotechnology, Yonsei University, Seoul 03722, Republic of Korea.

Tankyrases are multifunctional poly(ADP-ribose) polymerases that regulate a variety of cellular processes, including Wnt signaling, telomere maintenance and mitosis regulation. Tankyrases interact with target proteins and regulate their interactions and stability through poly(ADP-ribosyl) ation. In addition to their roles in telomere maintenance and regulation of mitosis, tankyrase proteins regulate tumor suppressors, including AXIN, phosphatase and tensin homolog and angiomotin. Read More

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December 2018

p53-Sensitive Epileptic Behavior and Inflammation in Hypomorphic Mice.

Front Genet 2018 27;9:581. Epub 2018 Nov 27.

Department of Biology and Biotechnology, Sapienza University of Rome, Rome, Italy.

Epilepsy is a complex clinical condition characterized by repeated spontaneous seizures. Seizures have been linked to multiple drivers including DNA damage accumulation. Investigation of epilepsy physiopathology in humans imposes ethical and practical limitations, for this reason model systems are mostly preferred. Read More

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November 2018

AaABF3, an Abscisic Acid-Responsive Transcription Factor, Positively Regulates Artemisinin Biosynthesis in .

Front Plant Sci 2018 28;9:1777. Epub 2018 Nov 28.

Joint International Research Laboratory of Metabolic and Developmental Sciences, Key Laboratory of Urban Agriculture (South) Ministry of Agriculture, Plant Biotechnology Research Center, Fudan-SJTU-Nottingham Plant Biotechnology R&D Center, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China.

Artemisinin is well known for its irreplaceable curative effect on the devastating parasitic disease, Malaria. This sesquiterpenoid is specifically produced in Chinese traditional herbal plant Earlier studies have shown that phytohormone abscisic acid (ABA) plays an important role in increasing the artemisinin content, but how ABA regulates artemisinin biosynthesis is still poorly understood. In this study, we identified that AaABF3 encoded an ABRE (ABA-responsive elements) binding factor. Read More

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November 2018

Plant Immunity Is Compartmentalized and Specialized in Roots.

Front Plant Sci 2018 28;9:1692. Epub 2018 Nov 28.

Normandie Univ, UNIROUEN, Laboratoire Glycobiologie et Matrice Extracellulaire Végétale EA4358, Rouen, France.

Roots are important organs for plant survival. In recent years, clear differences between roots and shoots in their respective plant defense strategies have been highlighted. Some putative gene markers of defense responses usually used in leaves are less relevant in roots and are sometimes not even expressed. Read More

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November 2018

Host Regulators of Liver Fibrosis During Human Schistosomiasis.

Front Immunol 2018 28;9:2781. Epub 2018 Nov 28.

Cape Town Component, International Centre for Genetic Engineering and Biotechnology, Cape Town, South Africa.

Liver fibrosis is a wound-healing process purposely aimed at restoring organ integrity after severe injury caused by autoimmune reactions, mechanical stress or infections. The uncontrolled solicitation of this process is pathogenic and a pathognomonic feature of diseases like hepatosplenic schistosomiasis where exacerbated liver fibrosis is centrally positioned among the drivers of the disease morbidity and mortality. Intriguingly, however, liver fibrosis occurs and progresses dissimilarly in schistosomiasis-diseased individuals with the same egg burden and biosocial features including age, duration of residence in the endemic site and gender. Read More

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November 2018
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Author Correction: The FAAH inhibitor URB597 suppresses hippocampal maximal dentate afterdischarges and restores seizure-induced impairment of short and long-term synaptic plasticity.

Sci Rep 2018 Dec 14;8(1):17977. Epub 2018 Dec 14.

Laboratory of Neurophysiology, Department of Physiology and Biochemistry, Faculty of Medicine and Surgery, University of Malta, Msida, Malta.

A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has not been fixed in the paper. Read More

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December 2018

Comparative genome analysis of marine purple sulfur bacterium Marichromatium gracile YL28 reveals the diverse nitrogen cycle mechanisms and habitat-specific traits.

Sci Rep 2018 Dec 13;8(1):17803. Epub 2018 Dec 13.

Department of Bioengineering and Biotechnology, Huaqiao University, Xiamen, 361021, China.

Mangrove ecosystems are characteristic of the high salinity, limited nutrients and S-richness. Marichromatium gracile YL28 (YL28) isolated from mangrove tolerates the high concentrations of nitrite and sulfur compounds and efficiently eliminates them. However, the molecular mechanisms of nitrite and sulfur compounds utilization and the habitat adaptation remain unclear in YL28. Read More

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December 2018

Reconstruction and analysis of carbon metabolic pathway of Ketogulonicigenium vulgare SPU B805 by genome and transcriptome.

Sci Rep 2018 Dec 13;8(1):17838. Epub 2018 Dec 13.

School of Life Science and Biopharmaceutics, Shenyang Pharmaceutical University, Shenyang, 110016, China.

Ketogulonicigenium vulgare has been widely used in vitamin C two-step fermentation. Four K. vulgare strains (WSH-001, Y25, Hbe602 and SKV) have been completely genome-sequenced, however, less attention was paid to elucidate the reason for the differences in 2-KGA yield on genetic level. Read More

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December 2018

Type I interferon/IRF7 axis instigates chemotherapy-induced immunological dormancy in breast cancer.

Oncogene 2018 Dec 13. Epub 2018 Dec 13.

Pathology Unit, Department of Oncology, Microbiology and Immunology (OMI), Faculty of Science and Medicine, University of Fribourg, 1700, Fribourg, Switzerland.

Neoadjuvant and adjuvant chemotherapies provide survival benefits to breast cancer patients, in particular in estrogen receptor negative (ER) cancers, by reducing rates of recurrences. It is assumed that the benefits of (neo)adjuvant chemotherapy are due to the killing of disseminated, residual cancer cells, however, there is no formal evidence for it. Here, we provide experimental evidence that ER breast cancer cells that survived high-dose Doxorubicin and Methotrexate based chemotherapies elicit a state of immunological dormancy. Read More

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December 2018

MicroRNAs derived from the insect virus HzNV-1 promote lytic infection by suppressing histone methylation.

Sci Rep 2018 Dec 13;8(1):17817. Epub 2018 Dec 13.

Department of Entomology, National Taiwan University, Taipei, 106, Taiwan.

Heliothis zea nudivirus-1 (HzNV-1) is an insect virus that can induce both lytic and latent infections in various insect cell lines. During latent infection, several microRNAs (miRNAs) are produced from persistency-associated gene 1 (pag1) as the only detectable HzNV-1 transcript. Previous studies have shown that the pag1 gene suppresses the immediate-early gene hhi1 and promotes host switching into a latent infection via miRNAs derived from pag1. Read More

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December 2018

Defending the return of results and data.

Science 2018 Dec 13;362(6420):1255-1256. Epub 2018 Dec 13.

Law Center, Department of Electrical and Computer Engineering, Center on Biotechnology and Law, University of Houston, Houston, TX 77204, USA.

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December 2018

Lactoferrin-loaded contact lenses counteract cytotoxicity caused in vitro by keratoconic tears.

Cont Lens Anterior Eye 2018 Dec 11. Epub 2018 Dec 11.

Department of Biotechnology and Biosciences, University of Milano - Bicocca, Piazza della Scienza 2, I-20126, Milan, Italy; Optic and Optometry Center of the University of Milano - Bicocca (COMiB), Italy.

Lactoferrin (LF), an iron-binding protein with antioxidant activity, is significantly reduced in the lacrimal film of patients affected by keratoconus (KC) compared to healthy subjects, and this is supposed to be the cause for tear iron increase and consequent iron deposition and oxygen free radical accumulation in the cornea. We decided to study if LF-loaded contact lenses (LF-CLs) could exert antioxidant activity on epithelial cells incubated in tears collected from two patients affected by keratoconus (KC1 and KC2). Moreover through this model we indirectly estimated iron concentration in the tears of healthy and KC subjects. Read More

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December 2018

In vitro effect of orexin A on the transcriptomic profile of the endometrium during early pregnancy in pigs.

Anim Reprod Sci 2018 Nov 20. Epub 2018 Nov 20.

Department of Animal Anatomy and Physiology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego 1A, 10-719 Olsztyn-Kortowo, Poland. Electronic address:

Orexin A belongs to the group of hypothalamic-derived peptides that are involved in a number of processes, such as regulation of energy metabolism, control of food intake and regulation of the reproductive system, by influencing the hypothalamic-pituitary-ovarian axis. Orexin A is also present in the endometrium, myometrium and placenta, which indicates that it may function as an important local regulator of the reproductive functions. The aim of this study was to explore the effects of orexin A on global gene expression in the endometrium of pigs during early gestation, on days 15 to 16 of pregnancy (implantation period). Read More

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November 2018

Asxl1 ablation in mouse embryonic stem cells impairs neural differentiation without affecting self-renewal.

Biochem Biophys Res Commun 2018 Dec 10. Epub 2018 Dec 10.

Department of Integrative Bioscience & Biotechnology, Sejong University, Seoul 05006, South Korea. Electronic address:

Additional sex comb-like1 (Asxl1) is known as a chromatin modulator that plays dual functions in transcriptional regulation depending on the cell type. Recent studies using Asxl1 knockout mice revealed that Asxl1 is important for the proliferation and differentiation of hematopoietic progenitor cells, and the development of organs. Although we previously reported Asxl1 as a Sox2 target gene, its function in embryonic stem cells (ESCs) remains largely unknown. Read More

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December 2018

Structural and functional properties of αβ-heterodimers of tropomyosin with myopathic mutations Q147P and K49del in the β-chain.

Biochem Biophys Res Commun 2018 Dec 10. Epub 2018 Dec 10.

A.N. Bach Institute of Biochemistry, Research Center of Biotechnology, Russian Academy of Sciences, Moscow, 119071, Russia; A.N. Belozersky Institute of Physico-Chemical Biology, Moscow State University, Moscow, 119234, Russia. Electronic address:

Tropomyosin (Tpm) is an α-helical coiled-coil actin-binding protein that plays a key role in the Ca-regulated contraction of striated muscles. Two Tpm isoforms, α (Tpm 1.1) and β (Tpm 2. Read More

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December 2018

A priori-defined Mediterranean-like dietary pattern predicts cardiovascular events better in north Europe than in Mediterranean countries.

Int J Cardiol 2018 Nov 29. Epub 2018 Nov 29.

Centro Cardiologico Monzino, IRCCS, Milan, Italy; Dipartimento di Scienze Farmacologiche e Biomolecolari, Università di Milano, Milan, Italy.

Background: The Mediterranean Diet (MD) is a model of healthy eating contributing to a favorable health status, but its clinical usefulness is still debated. The aim of this study was to relate the adherence to MD with the incidence of cardio/cerebro-vascular events (VEs) in north and south European participants of the IMPROVE study.

Methods: IMPROVE is an observational, longitudinal, prospective cohort study involving 3703 individuals from five European countries (Finland, Sweden, Netherlands, France and Italy). Read More

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November 2018

Xanthine oxidase inhibitors from an endophytic fungus Lasiodiplodia pseudotheobromae.

Bioorg Chem 2018 Dec 10. Epub 2018 Dec 10.

Department of Natural Products, National Institute of Pharmaceutical Education and Research (NIPER), Sector-67, S. A. S. Nagar 160 062, Punjab, India. Electronic address:

Two new compounds, lasdiplactone (1) and lasdiploic acid (2) and one known compound 3 were isolated from the chloroform extract of cell free filtrate of the endophytic fungus Lasiosdiplodia pseudotheobromae. The structures of new compounds were determined by interplay of spectral techniques (IR, mass, H NMR, C NMR, DEPT, and 2D NMR). The absolute configuration at C-4 position of 1 was established as S using a process similar to modified Mosher's method. Read More

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December 2018

Digynic triploidy in a fetus presenting with semilobar holoprosencephaly.

Taiwan J Obstet Gynecol 2018 Dec;57(6):881-884

Department of Medical Research, MacKay Memorial Hospital, Taipei, Taiwan; Department of Bioengineering, Tatung University, Taipei, Taiwan.

Objective: We present digynic triploidy in a fetus with semilobar holoprosencephaly (HPE).

Case Report: A 32-year-old, gravid 1, para 0, woman underwent prenatal ultrasound examination at 12 weeks of gestation, and the ultrasound showed relative macrocephaly, a small non-cystic placenta, and a fetus with absent nasal bone and semilobar HPE. The pregnancy was terminated subsequently, and a 50-g fetus was delivered with a relatively enlarged head and premaxillary agenesis. Read More

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December 2018

Impact of the mid-urethral sling for stress urinary incontinence on female sexual function and their partners' sexual activity.

Taiwan J Obstet Gynecol 2018 Dec;57(6):853-857

Department of Obstetrics and Gynecology, Far Eastern Memorial Hospital, Banqiao, New Taipei, Taiwan; Department of Obstetrics and Gynecology, National Taiwan University College of Medicine and the Hospital, Taipei, Taiwan. Electronic address:

Objective: To assess the impact of the transobturator mid-urethral sling (MUS) procedure on female sexual function and their partners' sexual activity.

Materials And Methods: Sexually active women with stress urinary incontinence who underwent a transobturator MUS procedure at the Department of Obstetrics and Gynecology of two medical centers were prospectively enrolled. All participants and their partners were required to complete questionnaires before surgery and 3 months after surgery. Read More

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December 2018

Effects of silymarin, cabergoline and letrozole on rat model of endometriosis.

Taiwan J Obstet Gynecol 2018 Dec;57(6):830-835

Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran. Electronic address:

Objective: Silymarin as an herbal drug has potent antioxidant effects that could make it a good choice for endometriosis therapy. The aim of the current study was to determine the effects of silymarin as an herbal drug on induced endometrial lesion in rat model of endometriosis.

Materials And Methods: A total of 32 mature, female Sprague-Dawley rats were allocated into 4 experimental groups. Read More

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December 2018
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Comprehensive analysis of oxidative stress markers and antioxidants status in preeclampsia.

Taiwan J Obstet Gynecol 2018 Dec;57(6):779-790

Department of Medical Biotechnology, Faculty of Medicine, Babol University of Medical Sciences, Babol, Iran.

Preeclampsia is a hypertensive disorder in pregnant women, which can be the leading cause of maternal and neonatal death or premature birth. Although the cause of preeclampsia is still not clear, local or systemic oxidative stress may explain the pathological features associated with this complication. However, it is not clear whether oxidative stress is the cause or the result of preeclampsia. Read More

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December 2018

Growth Signaling and Longevity in Mouse Models.

BMB Rep 2018 Dec 14. Epub 2018 Dec 14.

Institute of Animal Molecular Biotechnology, Korea University, Seoul 02841, Republic of Korea; Department of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul 02481, Republic of Korea.

Reduction of insulin/insulin-like growth factor 1 (IGF1) signaling (IIS) extends the lifespan of various species. So far, several longevity mouse models have been developed containing mutations related to growth signaling deficiency by targeting growth hormone (GH), IGF1, IGF1 receptor, insulin receptor, and insulin receptor substrate. In addition, p70 ribosomal protein S6 kinase 1 (S6K1) knockout leads to lifespan extension. Read More

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December 2018

Tat-ATOX1 inhibits inflammatory responses via regulation of MAPK and NF-κB pathways.

BMB Rep 2018 Dec 14. Epub 2018 Dec 14.

Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, Hallym University, Chuncheon 24252, Korea.

Antioxidant 1 (ATOX1) protein has been reported to exhibit various protective functions, including antioxidant and chaperone. However, the effects of ATOX1 on the inflammatory response has not been fully elucidated. Thus, we prepared cell permeable Tat-ATOX1 and studied the effects on lipopolysaccharide (LPS)- and 12-O-tetradecanoyl phorbol-13- acetate (TPA)-induced inflammation. Read More

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December 2018
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SETDB1 regulates SMAD7 expression for breast cancer metastasis.

BMB Rep 2018 Dec 14. Epub 2018 Dec 14.

Korea Research Institute of Bioscience and Biotechnology, Daejeon, 305-333, Republic of Korea; Department of Functional Genomics, Korea University of Science and Technology, Daejeon, 305-333, Republic of Korea.

Breast cancer (BRC) is the most invasive cancer in women. Although the survival rate of BRC is gradually increasing due to improved screening systems, development of novel therapeutic targets for inhibition of BRC proliferation, metastasis and recurrence have been constantly needed. Thus, in this study, we identified overexpression of SETDB1 (SET Domain Bifurcated 1), a histone methyltransferase, in RNA-seq data of BRC derived from TCGA portal. Read More

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December 2018

Nitrogen topdressing timing modifies free amino acids profiles and storage protein gene expression in wheat grain.

BMC Plant Biol 2018 Dec 13;18(1):353. Epub 2018 Dec 13.

National Technique Innovation Center for Regional Wheat Production / Key Laboratory of Crop Physiology and Ecology in Southern China, Ministry of Agriculture / National Engineering and technology Center for Information Agriculture, Nanjing Agricultural University, Nanjing, People's Republic of China.

Background: Nitrogen is one basic element of amino acids and grain protein in wheat. In field experiments, wheat plants were subjected to different timing of nitrogen topdressing treatments: at the stages of emergence of the top fifth leaf (TL5), top third leaf (TL3) and top first leaf (TL1) to test the regulatory effects of nitrogen topdressing timing on grain protein quality. The underlying mechanisms were elucidated by clarifying the relationship between proteolysis in vegetative organs and accumulation of amino acids in the endosperm cavity, conversion of amino acids, and storage protein synthesis in endosperm of wheat grain. Read More

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December 2018

The optimality of the standard genetic code assessed by an eight-objective evolutionary algorithm.

BMC Evol Biol 2018 Dec 13;18(1):192. Epub 2018 Dec 13.

Department of Genomics, Faculty of Biotechnology, University of Wrocław, ul. Joliot-Curie 14a, 50-383, Wrocław, Poland.

Background: The standard genetic code (SGC) is a unique set of rules which assign amino acids to codons. Similar amino acids tend to have similar codons indicating that the code evolved to minimize the costs of amino acid replacements in proteins, caused by mutations or translational errors. However, if such optimization in fact occurred, many different properties of amino acids must have been taken into account during the code evolution. Read More

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December 2018

Global Methylome and gene expression analysis during early Peanut pod development.

BMC Plant Biol 2018 Dec 13;18(1):352. Epub 2018 Dec 13.

Biotechnology Research Center, Shandong Academy of Agricultural Sciences; Shandong Provincial Key Laboratory of Crop Genetic Improvement, Ecology and Physiology, Jinan, 250100, People's Republic of China.

Background: Early peanut pod development is an important process of peanut reproductive development. Modes of DNA methylation during early peanut pod development are still unclear, possibly because its allotetraploid genome may cause difficulty for the methylome analysis.

Results: To investigate the functions of the dynamic DNA methylation during the early development of the peanut pod, global methylome and gene expression analyses were carried out by Illumina high throughput sequencing. Read More

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December 2018
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Impact of index hopping and bias towards the reference allele on accuracy of genotype calls from low-coverage sequencing.

Genet Sel Evol 2018 Dec 13;50(1):64. Epub 2018 Dec 13.

The Roslin Institute and Royal (Dick) School of Veterinary Studies, The University of Edinburgh, Easter Bush, Midlothian, Scotland, UK.

Background: Inherent sources of error and bias that affect the quality of sequence data include index hopping and bias towards the reference allele. The impact of these artefacts is likely greater for low-coverage data than for high-coverage data because low-coverage data has scant information and many standard tools for processing sequence data were designed for high-coverage data. With the proliferation of cost-effective low-coverage sequencing, there is a need to understand the impact of these errors and bias on resulting genotype calls from low-coverage sequencing. Read More

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December 2018

High-Throughput Electrophysiology Screen Revealed Cardiotoxicity of Strychnine by Selectively Targeting hERG Channel.

Am J Chin Med 2018 Dec 14:1-16. Epub 2018 Dec 14.

1 Tianjin State Key Laboratory of Modern, Chinese Medicine, Tianjin University of Traditional, Chinese Medicine, Tianjin 300193, P. R. China.

Although the efficacy and the health care advantages of Chinese herbal medicine (CHM) have become increasingly recognized worldwide, the potential side effects and toxicity still restrict its broader application. This study established and applied an integrated platform anchored on automatic patch clamp system to screen and evaluate a collection of CHM extracts, compositions and monomeric compounds for in vitro cardiac toxicity. Of 1036 CHM samples screened, 2. Read More

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December 2018
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Analysis of Trauma Intensity during Surgical Bone Procedures Using NF-κB Expression Levels as a Stress Sensor: An Experimental Study in a Wistar Rat Model.

Materials (Basel) 2018 Dec 12;11(12). Epub 2018 Dec 12.

Department of Biotechnology, Biotecnos-Technology and Science, Cuareim 1483, Montevideo, Canelones 11100, Uruguay.

It is well known that the transcription factor NF-κB regulates multiple aspects of innate and adaptive immune functions and functions as a pivotal mediator of inflammatory responses. In the present study, we evaluated the trauma generated (inflammatory reaction) after osteotomy bone surgical procedures and placement of implants in the femoral cortical bone of Wistar rats. Surgical stress was evaluated measuring the release and activation of the NF-κB factor. Read More

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December 2018

Parechovirus A Detection by a Comprehensive Approach in a Clinical Laboratory.

Viruses 2018 Dec 12;10(12). Epub 2018 Dec 12.

Department of Medical Education and Research, Kaohsiung Veterans General Hospital, Kaohsiung 81362, Taiwan.

Parechovirus A (Human parechovirus, HPeV) causes symptoms ranging from severe neonatal infection to mild gastrointestinal and respiratory disease. Use of molecular approaches with RT-PCR and genotyping has improved the detection rate of HPeV. Conventional methods, such as viral culture and immunofluorescence assay, together with molecular methods facilitate comprehensive viral diagnosis. Read More

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December 2018
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Intestinal Anti-Inflammatory Activity of the Aqueous Extract from Ipomoea asarifolia in DNBS-Induced Colitis in Rats.

Int J Mol Sci 2018 Dec 12;19(12). Epub 2018 Dec 12.

Department of Biophysics and Pharmacology, Biosciences Center, Federal University of Rio Grande do Norte, Natal 59072-970, Brazil.

Inflammatory bowel disease is triggered by an uncontrolled immune response associated with genetic, environmental, and intestinal microbiota imbalance. Ipomoea asarifolia (IA), popularly known as "salsa" or "brave salsa", belongs to the Convolvulaceae family. The aim of this approach was to study the preventive effect of IA aqueous extract in 2,4-dinitrobenzene sulfonic acid (DNBS)-induced colitis in rats. Read More

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December 2018

Proteogenomic Analysis of Species Strains 25 and 46 Isolated from Uraniferous Soils Reveals Multiple Mechanisms to Cope with Uranium Stress.

Cells 2018 Dec 12;7(12). Epub 2018 Dec 12.

Environmental Biotechnology Laboratory, School of the Environment, 1515 S. Martin Luther King Jr. Blvd., Suite 305B, FSH Science Research Center, Florida A&M University, Tallahassee, FL 32307, USA.

Two spp. (strains SRS-25 and SRS-46) were isolated from high concentrations of uranium (U) from the U.S. Read More

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December 2018