1,659 results match your criteria Biotechnology Advances [Journal]


Amylosucrase as a transglucosylation tool: From molecular features to bioengineering applications.

Biotechnol Adv 2018 Jun 20. Epub 2018 Jun 20.

State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China; International Joint Laboratory on Food Safety, Jiangnan University, Wuxi, Jiangsu 214122, China. Electronic address:

Amylosucrase (EC 2.4.1. Read More

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June 2018
9 Reads

Fructans of the saline world.

Biotechnol Adv 2018 Jun 20. Epub 2018 Jun 20.

Industrial Biotechnology and Systems Biology Research Group, Marmara University, Bioengineering Department, 34722 Istanbul, Turkey.

Saline and hypersaline environments make up the largest ecosystem on earth and the organisms living in such water-restricted environments have developed unique ways to cope with high salinity. As such these organisms not only carry significant industrial potential in a world where freshwater supplies are rapidly diminishing, but they also shed light upon the origins and extremes of life. One largely overlooked and potentially important feature of many salt-loving organisms is their ability to produce fructans, fructose polymers widely found in various mesophilic Eubacteria and plants, with potential functions as storage carbohydrates, aiding stress tolerance, and acting as virulence factors or signaling molecules. Read More

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Supplementing glycosylation: A review of applying nucleotide-sugar precursors to growth medium to affect therapeutic recombinant protein glycoform distributions.

Biotechnol Adv 2018 Jun 15. Epub 2018 Jun 15.

Centre for Biotechnology and Bioengineering, Department of Chemical Engineering, University of Waterloo, Waterloo, Ontario N2L 3G1, Canada. Electronic address:

Glycosylation is a critical quality attribute (CQA) of many therapeutic proteins, particularly monoclonal antibodies (mAbs), and is a major consideration in the approval of biosimilar biologics due to its effects to therapeutic efficacy. Glycosylation generates a distribution of glycoforms, resulting in glycoproteins with inherent molecule-to-molecule heterogeneity, capable of activating (or failing to activate) different effector functions of the immune system. Glycoforms can be affected by the supplementation of nucleotide-sugar precursors, and related components, to culture growth medium, affecting the metabolism of glycosylation. Read More

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June 2018
1 Read

Essential steps in bioprinting: From pre- to post-bioprinting.

Biotechnol Adv 2018 Jun 25. Epub 2018 Jun 25.

Engineering Science and Mechanics Department, Penn State University, University Park, PA 16802, USA; The Huck Institutes of the Life Sciences, Penn State University, University Park, PA 16802, USA; Biomedical Engineering Department, Penn State University, University Park, PA 16802, USA; Materials Research Institute, Penn State University, University Park, PA 16802, USA. Electronic address:

An increasing demand for directed assembly of biomaterials has inspired the development of bioprinting, which facilitates the assembling of both cellular and acellular inks into well-arranged three-dimensional (3D) structures for tissue fabrication. Although great advances have been achieved in the recent decade, there still exist issues to be addressed. Herein, a review has been systematically performed to discuss the considerations in the entire procedure of bioprinting. Read More

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Integrating enzyme immobilization and protein engineering: An alternative path for the development of novel and improved industrial biocatalysts.

Biotechnol Adv 2018 Jun 9. Epub 2018 Jun 9.

Laboratorio de Tecnología de Enzimas para Bioprocesos, Departamento de Ingeniería en Alimentos, Universidad de La Serena, Av. Raúl Bitrán 1305, 1720010 La Serena, Chile; Instituto de Investigación Multidisciplinario en Ciencia y Tecnología, Universidad de La Serena, Benavente 980, 1720010 La Serena, Chile. Electronic address:

Enzyme immobilization often achieves reusable biocatalysts with improved operational stability and solvent resistance. However, these modifications are generally associated with a decrease in activity or detrimental modifications in catalytic properties. On the other hand, protein engineering aims to generate enzymes with increased performance at specific conditions by means of genetic manipulation, directed evolution and rational design. Read More

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June 2018
5 Reads

Pre-treatments to enhance the biodegradability of waste activated sludge: Elucidating the rate limiting step.

Biotechnol Adv 2018 Jun 7. Epub 2018 Jun 7.

Faculty of Civil Engineering and Geosciences, Department of Water Management, Sanitary Engineering Section, Delft University of Technology (TU Delft), Stevinweg 1, 2628, CN, Delft, the Netherlands.

Pre-treatments for waste activated sludge (WAS) are, in most cases, an attempt to increase the biodegradation and/or improve hydrolysis rate of WAS after anaerobic digestion. This review presents an extensive analysis of WAS pre-treatments effectiveness focusing on increasing the biodegradability. In the first part of the review, WAS is considered as a cluster of organic components: proteins, carbohydrates, humic substances and cells. Read More

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Screening of organic solvents for bioprocesses using aqueous-organic two-phase systems.

Biotechnol Adv 2018 Jun 2. Epub 2018 Jun 2.

Center for Process and Systems Engineering (PROSYS), Department of Chemical and Biochemical Engineering, Technical University of Denmark (DTU), Kgs Lyngby DK-2800, Denmark. Electronic address:

The application of conventional organic solvents has been essential in several steps of bioprocesses in order to achieve sufficient economic efficiency. The use of organic solvents is frequently used either to partly or fully replace water in the reaction medium or as a process aid for downstream separation. Nowadays, manufacturers are increasingly requested to avoid and substitute solvents with hazardous potential. Read More

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Application of redox mediators in bioelectrochemical systems.

Biotechnol Adv 2018 May 29. Epub 2018 May 29.

Instituto Tecnologico de Sonora (ITSON), Departamento de Ciencias Agronomicas y Veterinarias, 5 de Febrero 818 Sur C.P., 85000 Cuidad Obregon, Sonora, Mexico. Electronic address:

Redox mediators (RM) are natural or artificial compounds used by microorganisms as electron acceptors and electron donors during electron transfer. Evidence collected in the last years indicates that the application of RM in bioelectrochemical systems (BES) enhanced the electron transfer from microorganisms to anodes and from cathodes to microorganisms. This review summarizes the results of using soluble or immobilized RM in BES to produce electricity and for the treatment of contaminants from wastewater effluents. Read More

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Genetic and metabolic engineering for microbial production of poly-γ-glutamic acid.

Biotechnol Adv 2018 May 28. Epub 2018 May 28.

School of Engineering, Massey University, Private Bag 11 222, Palmerston North, New Zealand. Electronic address:

Poly-γ-glutamic acid (γ-PGA) is a natural biopolymer of glutamic acid. The repeating units of γ-PGA may be derived exclusively from d-glutamic acid, or l-glutamic acid, or both. The monomer units are linked by amide bonds between the α-amino group and the γ-carboxylic acid group. Read More

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May 2018
3 Reads

Silver bullets: A new lustre on an old antimicrobial agent.

Biotechnol Adv 2018 May 27. Epub 2018 May 27.

The University of Queensland, Institute for Molecular Bioscience, St Lucia 4072, Australia. Electronic address:

Silver was widely used in medicine to treat bacterial infections in the 19th and early 20th century, up until the discovery and development of the first modern antibiotics in the 1940s, which were markedly more effective. Since then, every new antibiotic introduced to the clinic has led to an associated development of drug resistance. Today, the threat of extensive bacterial resistance to antibiotics has reignited interest in alternative strategies to treat infectious diseases, with silver regaining well-deserved renewed attention. Read More

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Circulating tumor microemboli: Progress in molecular understanding and enrichment technologies.

Biotechnol Adv 2018 Jul - Aug;36(4):1367-1389. Epub 2018 May 18.

Queensland Micro- and Nanotechnology Centre (QMNC), Griffith University, Nathan Campus, QLD 4111, Australia; School of Environment and Science, Griffith University, Nathan Campus, QLD 4111, Australia. Electronic address:

Circulating tumor cells (CTCs) and their clusters, also known as circulating tumor microemboli (CTM), have emerged as valuable tool that can provide mechanistic insights into the tumor heterogeneity, clonal evolution, and stochastic events within the metastatic cascade. However, recent investigations have hinted that CTM may not be mere aggregates of tumor cells but cells comprising CTM exhibit distinct phenotypic and molecular characteristics in comparison to single CTCs. Moreover, in many cases CTM demonstrated higher metastatic potential and resistance to apoptosis as compared to their single cell counterparts. Read More

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Guest Editorial Prospects in Biotechnology.

Biotechnol Adv 2018 May - Jun;36(3):555-556

School of Engineering, Massey University, Private Bag 11 222, Palmerston North, New Zealand. Electronic address:

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May 2018
1 Read

Perfusion mammalian cell culture for recombinant protein manufacturing - A critical review.

Biotechnol Adv 2018 Jul - Aug;36(4):1328-1340. Epub 2018 May 5.

Institute of Chemical and Bioengineering, ETH Zürich, Zürich, Switzerland. Electronic address:

The manufacturing of recombinant protein is traditionally divided in two main steps: upstream (cell culture and synthesis of the target protein) and downstream (purification and formulation of the protein into a drug substance or drug product). Today, cost pressure, market uncertainty and market growth, challenge the existing manufacturing technologies. Leaders in the field are active in designing the process of the future and continuous manufacturing is recurrently mentioned as a potential solution to address some of the current limitations. Read More

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"Connecting worlds - a view on microfluidics for a wider application".

Biotechnol Adv 2018 Jul - Aug;36(4):1341-1366. Epub 2018 May 4.

Process and Systems Engineering Center (PROSYS), Department of Chemical and Biochemical Engineering, Technical University of Denmark, Building 229, 2800 Kgs. Lyngby, Denmark.

From its birth, microfluidics has been referenced as a revolutionary technology and the solution to long standing technological and sociological issues, such as detection of dilute compounds and personalized healthcare. Microfluidics has for example been envisioned as: (1) being capable of miniaturizing industrial production plants, thereby increasing their automation and operational safety at low cost; (2) being able to identify rare diseases by running bioanalytics directly on the patient's skin; (3) allowing health diagnostics in point-of-care sites through cheap lab-on-a-chip devices. However, the current state of microfluidics, although technologically advanced, has so far failed to reach the originally promised widespread use. Read More

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May 2018
11 Reads

Natural scaffolds in anticancer therapy and precision medicine.

Biotechnol Adv 2018 May 3. Epub 2018 May 3.

Department of Pharmacy, College of Pharmacy, Seoul National University, Seoul 151-742, South Korea. Electronic address:

The diversity of natural compounds is essential for their mechanism of action. The source, structures and structure activity relationship of natural compounds contributed to the development of new classes of chemotherapy agents for over 40 years. The availability of combinatorial chemistry and high-throughput screening has fueled the challenge to identify novel compounds that mimic nature's chemistry and to predict their macromolecular targets. Read More

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Leveraging knowledge engineering and machine learning for microbial bio-manufacturing.

Biotechnol Adv 2018 Jul - Aug;36(4):1308-1315. Epub 2018 May 3.

Department of Energy, Environmental and Chemical Engineering, Washington University in Saint Louis, Saint Louis, MO 63130, USA. Electronic address:

Genome scale modeling (GSM) predicts the performance of microbial workhorses and helps identify beneficial gene targets. GSM integrated with intracellular flux dynamics, omics, and thermodynamics have shown remarkable progress in both elucidating complex cellular phenomena and computational strain design (CSD). Nonetheless, these models still show high uncertainty due to a poor understanding of innate pathway regulations, metabolic burdens, and other factors (such as stress tolerance and metabolite channeling). Read More

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Toolboxes for cyanobacteria: Recent advances and future direction.

Biotechnol Adv 2018 Jul - Aug;36(4):1293-1307. Epub 2018 May 3.

Laboratory of Synthetic Microbiology, School of Chemical Engineering & Technology, Tianjin University, Tianjin 300072, PR China; Key Laboratory of Systems Bioengineering (Ministry of Education), Tianjin University, Tianjin 300072, PR China; SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering, Tianjin 300072, PR China; Center for Biosafety Research and Strategy, Tianjin University, Tianjin, PR China.

Photosynthetic cyanobacteria are important primary producers and model organisms for studying photosynthesis and elements cycling on earth. Due to the ability to absorb sunlight and utilize carbon dioxide, cyanobacteria have also been proposed as renewable chassis for carbon-neutral "microbial cell factories". Recent progresses on cyanobacterial synthetic biology have led to the successful production of more than two dozen of fuels and fine chemicals directly from CO, demonstrating their potential for scale-up application in the future. Read More

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May 2018
1 Read

Microbial fuel cell (MFC) power performance improvement through enhanced microbial electrogenicity.

Biotechnol Adv 2018 Jul - Aug;36(4):1316-1327. Epub 2018 May 3.

Key Laboratory of Pollution Process and Environmental Criteria, Ministry of Education, Tianjin Key Laboratory of Urban Ecology Environmental Remediation and Pollution Control, Tianjin Advanced Water Treatment Technology International Joint Research Center, College of Environmental Science and Engineering, Nankai University, Tianjin 300071, PR China. Electronic address:

Within the past 5 years, tremendous advances have been made to maximize the performance of microbial fuel cells (MFCs) for both "clean" bioenergy production and bioremediation. Most research efforts have focused on parameters including (i) optimizing reactor configuration, (ii) electrode construction, (iii) addition of redox-active, electron donating mediators, (iv) biofilm acclimation and feed nutrient adjustment, as well as (v) other parameters that contribute to enhanced MFC performance. To date, tremendous advances have been made, but further improvements are needed for MFCs to be economically practical. Read More

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Vascular smooth muscle cell proliferation as a therapeutic target. Part 1: molecular targets and pathways.

Biotechnol Adv 2018 Apr 21. Epub 2018 Apr 21.

Department of Molecular Biology, Institute of Genetics and Animal Breeding of the Polish Academy of Sciences, ul. Postepu 36A, Jastrzębiec, 05-552 Magdalenka, Poland; Department of Pharmacognosy, University of Vienna, Althanstrasse 14, A-1090 Vienna, Austria. Electronic address:

Cardiovascular diseases are a major cause of human death worldwide. Excessive proliferation of vascular smooth muscle cells contributes to the etiology of such diseases, including atherosclerosis, restenosis, and pulmonary hypertension. The control of vascular cell proliferation is complex and encompasses interactions of many regulatory molecules and signaling pathways. Read More

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April 2018
1 Read

Vascular smooth muscle cell proliferation as a therapeutic target. Part 2: Natural products inhibiting proliferation.

Biotechnol Adv 2018 Apr 17. Epub 2018 Apr 17.

Department of Molecular Biology, Institute of Genetics and Animal Breeding of the Polish Academy of Sciences, ul. Postepu 36A, Magdalenka 05552, Poland; Department of Pharmacognosy, University of Vienna, Althanstrasse 14, Vienna 1090, Austria. Electronic address:

Many natural products have been so far tested regarding their potency to inhibit vascular smooth muscle cell proliferation, a process involved in atherosclerosis, pulmonary hypertension and restenosis. Compounds studied in vitro and in vivo as VSMC proliferation inhibitors include, for example indirubin-3'-monoxime, resveratrol, hyperoside, plumericin, pelargonidin, zerumbone and apamin. Moreover, taxol and rapamycin, the most prominent compounds applied in drug-eluting stents to counteract restenosis, are natural products. Read More

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April 2018
1 Read

Leveraging algal omics to reveal potential targets for augmenting TAG accumulation.

Biotechnol Adv 2018 Jul - Aug;36(4):1274-1292. Epub 2018 Apr 18.

National Bioenergy Center, National Renewable Energy Laboratory, Golden, CO 80401, USA. Electronic address:

Ongoing global efforts to commercialize microalgal biofuels have expedited the use of multi-omics techniques to gain insights into lipid biosynthetic pathways. Functional genomics analyses have recently been employed to complement existing sequence-level omics studies, shedding light on the dynamics of lipid synthesis and its interplay with other cellular metabolic pathways, thus revealing possible targets for metabolic engineering. Here, we review the current status of algal omics studies to reveal potential targets to augment TAG accumulation in various microalgae. Read More

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April 2018
1 Read

Using agro-industrial wastes for the cultivation of microalgae and duckweeds: Contamination risks and biomass safety concerns.

Biotechnol Adv 2018 Jul - Aug;36(4):1238-1254. Epub 2018 Apr 17.

School of Science and the Environment, Memorial University of Newfoundland, 20 University Drive, Corner Brook, NL A2H 5G4, Canada.

Aquatic organisms, such as microalgae (Chlorella, Arthrospira (Spirulina), Tetrasselmis, Dunalliela etc.) and duckweed (Lemna spp., Wolffia spp. Read More

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April 2018
3 Reads

Microalgae as multi-functional options in modern agriculture: current trends, prospects and challenges.

Biotechnol Adv 2018 Jul - Aug;36(4):1255-1273. Epub 2018 Apr 17.

Institute for Water and Wastewater Technology, Durban University of Technology, P.O. Box 1334, Durban 4000, South Africa. Electronic address:

Algae are a group of ubiquitous photosynthetic organisms comprising eukaryotic green algae and Gram-negative prokaryotic cyanobacteria, which have immense potential as a bioresource for various industries related to biofuels, pharmaceuticals, nutraceuticals and feed. This fascinating group of organisms also has applications in modern agriculture through facilitating increased nutrient availability, maintaining the organic carbon and fertility of soil, and enhancing plant growth and crop yields, as a result of stimulation of soil microbial activity. Several cyanobacteria provide nitrogen fertilization through biological nitrogen fixation and through enzymatic activities related to interconversions and mobilization of different forms of nitrogen. Read More

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April 2018
1 Read

Naturally occurring compounds in differentiation based therapy of cancer.

Biotechnol Adv 2018 Apr 12. Epub 2018 Apr 12.

Department of Immunology, Institute for Biological Research "Siniša Stanković", University of Belgrade, Bulevar Despota Stefana 142, 11060 Belgrade, Serbia.

Differentiation of cancer cells entails the reversion of phenotype from malignant to the original. The conversion to cell type characteristic for another tissue is named transdifferentiation. Differentiation/transdifferentiation of malignant cells in high grade tumor mass could serve as a nonaggressive approach that potentially limits tumor progression and augments chemosensitivity. Read More

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April 2018
1 Read

Host cell protein removal from biopharmaceutical preparations: Towards the implementation of quality by design.

Biotechnol Adv 2018 Jul - Aug;36(4):1223-1237. Epub 2018 Apr 11.

Department of Chemical Engineering, Imperial College London, London, United Kingdom. Electronic address:

Downstream processing of protein products of mammalian cell culture currently accounts for the largest fraction of the total production cost. A major challenge is the removal of host cell proteins, which are cell-derived impurities. Host cell proteins are potentially immunogenic and can compromise product integrity during processing and hold-up steps. Read More

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April 2018
3 Reads

Electron transport chains in organohalide-respiring bacteria and bioremediation implications.

Biotechnol Adv 2018 Jul - Aug;36(4):1194-1206. Epub 2018 Apr 6.

School of Environmental Science and Engineering, Sun Yat-Sen University, Guangzhou 510275, China; Guangdong Provincial Key Laboratory of Environmental Pollution Control and Remediation Technology, Guangzhou 510275, China.

In situ remediation employing organohalide-respiring bacteria represents a promising solution for cleanup of persistent organohalide pollutants. The organohalide-respiring bacteria conserve energy by utilizing H or organic compounds as electron donors and organohalides as electron acceptors. Reductive dehalogenase (RDase), a terminal reductase of the electron transport chain in organohalide-respiring bacteria, is the key enzyme that catalyzes halogen removal. Read More

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April 2018
6 Reads

Aspergillus-specific antibodies - Targets and applications.

Biotechnol Adv 2018 Jul - Aug;36(4):1167-1184. Epub 2018 Mar 30.

Fraunhofer Institute for Molecular Biology and Applied Ecology IME, Forckenbeckstrasse 6, 52074 Aachen, Germany.

Aspergillus is a fungal genus comprising several hundred species, many of which can damage the health of plants, animals and humans by direct infection and/or due to the production of toxic secondary metabolites known as mycotoxins. Aspergillus-specific antibodies have been generated against polypeptides, polysaccharides and secondary metabolites found in the cell wall or secretions, and these can be used to detect and monitor infections or to quantify mycotoxin contamination in food and feed. However, most Aspergillus-specific antibodies are generated against heterogeneous antigen preparations and the specific target remains unknown. Read More

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March 2018
2 Reads

Process engineering for microbial production of 3-hydroxypropionic acid.

Biotechnol Adv 2018 Jul - Aug;36(4):1207-1222. Epub 2018 Mar 31.

UMR 782 GMPA, AgroParisTech, INRA, Université Paris Saclay, 78850 Thiverval-Grignon, France. Electronic address:

Due to concerns about the unsustainability and predictable shortage of fossil feedstocks, research efforts are currently being made to develop new processes for production of commodities using alternative feedstocks. 3-Hydroxypropionic acid (CAS 503-66-2) was recognised by the US Department of Energy as one of the most promising value-added chemicals that can be obtained from biomass. This article aims at reviewing the various strategies implemented thus far for 3-hydroxypropionic acid bioproduction. Read More

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March 2018
1 Read

Recent advances on production of 2, 3-butanediol using engineered microbes.

Biotechnol Adv 2018 Mar 31. Epub 2018 Mar 31.

Department of Chemical and Biological Engineering, University of Ottawa, 161 Louis Pasteur Private, Ottawa, ON K1N 6N5, Canada. Electronic address:

As a significant platform chemical, 2, 3-butanediol (2, 3-BD) has found wide applications in industry. The success of microbial 2, 3-BD production was limited by the use of pathogenic microorganisms and low titer in engineered hosts. The utilization of cheaply available feedstock such as lignocellulose was another major challenge to achieve economic production of 2, 3-BD. Read More

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March 2018
1 Read

Clinical, technological, and economic issues associated with developing new lung surfactant therapeutics.

Biotechnol Adv 2018 Jul - Aug;36(4):1185-1193. Epub 2018 Mar 26.

School of Chemical Engineering, Purdue University, West Lafayette, IN 47907, United States. Electronic address:

Discovery of lung surfactant deficiency as a main cause of neonatal respiratory distress syndrome (NRDS) has influenced a steep increase in lung surfactant research. Although this has yielded impactful scientific discoveries, much of the basic research on lung surfactants has failed to translate into clinical practices. This is attributed to insufficient information covering the entire lung surfactant ecosystem, from the basic science to economics surrounding the development and clinical practices. Read More

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March 2018
1 Read

State of the art of aerobic granulation in continuous flow bioreactors.

Biotechnol Adv 2018 Jul - Aug;36(4):1139-1166. Epub 2018 Mar 26.

Occoquan Laboratory, Department of Civil and Environmental Engineering, Virginia Tech, United States. Electronic address:

In the wake of the success of aerobic granulation in sequential batch reactors (SBRs) for treating wastewater, attention is beginning to turn to continuous flow applications. This is a necessary step given the advantages of continuous flow treatment processes and the fact that the majority of full-scale wastewater treatment plants across the world are operated with aeration tanks and clarifiers in a continuous flow mode. As in SBRs, applying a selection pressure, based on differences in either settling velocity or the size of the biomass, is essential for successful granulation in continuous flow reactors (CFRs). Read More

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March 2018
1 Read

Dietary nutraceuticals as backbone for bone health.

Biotechnol Adv 2018 Mar 27. Epub 2018 Mar 27.

Inflammation Research Center, San Diego, CA, USA.

Bone loss or osteoporosis, is a slow-progressing disease that results from dysregulation of pro-inflammatory cytokines. The FDA has approved number of drugs for bone loss prevention, nonetheless all are expensive and have multiple side effects. The nutraceuticals identified from dietary agents such as butein, cardamonin, coronarin D curcumin, diosgenin, embelin, gambogic acid, genistein, plumbagin, quercetin, reseveratrol, zerumbone and more, can modulate cell signaling pathways and reverse/slow down osteoporosis. Read More

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March 2018
3 Reads

Revisiting glycoside hydrolase family 20 β-N-acetyl-d-hexosaminidases: Crystal structures, physiological substrates and specific inhibitors.

Biotechnol Adv 2018 Jul - Aug;36(4):1127-1138. Epub 2018 Mar 26.

State Key Laboratory for Biology of Plant Diseases and Insect Pests, Dalian University of Technology, Dalian 116024, China. Electronic address:

Glycoside hydrolase family 20 β-N-acetyl-d-hexosaminidases (GH20s) catalyze the hydrolysis of glycosidic linkages in glycans, glycoproteins and glycolipids. The diverse substrates of GH20s account for their various roles in many important bioprocesses, such as glycoprotein modification, glycoconjugate metabolism, gamete recognition and chitin degradation in fungal cell walls and arthropod exoskeletons. Defects in human GH20s cause lysosomal storage diseases, Alzheimer's disease and osteoarthritis. Read More

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March 2018
3 Reads

Natural products with anti-aging potential: Affected targets and molecular mechanisms.

Biotechnol Adv 2018 Mar 27. Epub 2018 Mar 27.

MEDFUTURE - Research Center for Advanced Medicine, 400012 Cluj-Napoca, Romania; Research Center for Functional Genomics, Biomedicine and Translational Medicine, Institute of Doctoral Studies, "Iuliu Hatieganu" University of Medicine and Pharmacy, 400012 Cluj-Napoca, Romania; Department of Experimental Pathology, "Prof. Dr. Ion Chiricuta", The Oncology Institute, Cluj-Napoca, Romania.

In recent years, there has been a great deal of attention toward the molecular machinery relevant to age-related progression controlled through the external intervention of polyphenols- an epigenetic-modulating diet. Natural products modulate cellular longevity through histone post-translational modification and can also induce the upregulation of autophagy, thus reducing the level of acetyl coenzyme A (AcCoA). In addition, the effect of caloric restriction (CR) on cancer-related chronic inflammation is of great significance in aging. Read More

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March 2018
2 Reads

A state-of-the-art review on nitrous oxide control from waste treatment and industrial sources.

Biotechnol Adv 2018 Jul - Aug;36(4):1025-1037. Epub 2018 Mar 20.

Department of Chemical Engineering and Environmental Technology, University of Valladolid, Dr. Mergelina, s/n, Valladolid 47011, Spain. Electronic address:

This review aims at holistically analyzing the environmental problems associated with nitrous oxide (NO) emissions by evaluating the most important sources of NO and its environmental impacts. Emissions from wastewater treatment processes and the industrial production of nitric and adipic acid represent nowadays the most important anthropogenic point sources of NO. Therefore, state-of-the-art strategies to mitigate the generation and release to the atmosphere of this greenhouse and O-depleting gas in the waste treatment and industrial sectors are also reviewed. Read More

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March 2018
1 Read

Stem cells: their source, potency and use in regenerative therapies with focus on adipose-derived stem cells - a review.

Biotechnol Adv 2018 Jul - Aug;36(4):1111-1126. Epub 2018 Mar 18.

Clinic of Plastic Surgery, Faculty Hospital Na Bulovce, Budinova 67/2, 180 81 Prague, 8-Liben, Czech Republic.

Stem cells can be defined as units of biological organization that are responsible for the development and the regeneration of organ and tissue systems. They are able to renew their populations and to differentiate into multiple cell lineages. Therefore, these cells have great potential in advanced tissue engineering and cell therapies. Read More

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March 2018
4 Reads

Investigations into the cancer stem cell niche using in-vitro 3-D tumor models and microfluidics.

Biotechnol Adv 2018 Jul - Aug;36(4):1094-1110. Epub 2018 Mar 17.

Department of Chemical Engineering, Indian Institute of Science, Bangalore 560012, India. Electronic address:

The concept of Cancer Stem Cells (CSCs) and the CSC Niche/Tumor Microenvironment (TME) as the central driving force behind tumor progression and maintenance has garnered much attention in recent years. Concomitantly, the widespread adoption of 3D tissue models, organotypic co-cultures, and the revolutionary microfluidic technology has resulted in a plethora of ground-breaking fundamental discoveries and has enabled investigations which were previously unfeasible. A large number of existing review papers concern themselves with either a broad look at the TME and CSC Niche, or on the studies undertaken on a particular niche component alone. Read More

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March 2018
1 Read

Biofiltration of volatile organic compounds using fungi and its conceptual and mathematical modeling.

Biotechnol Adv 2018 Jul - Aug;36(4):1079-1093. Epub 2018 Mar 17.

Green Technology Research Group, Facultad de Ingeniería y Ciencias Aplicadas, Universidad de los Andes, Chile.

Volatile organic compounds (VOCs) are ubiquitous contaminants that can be found both in outdoor and indoor air, posing risks to human health and the ecosystems. The treatment of air contaminated with VOCs in low concentrations can be effectively performed using biofiltration, especially when VOCs are hydrophilic. However, the performance of biofilters inoculated with bacteria has been found to be low with sparsely water soluble molecules when compared to biofilters where fungi develop. Read More

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March 2018
4 Reads

Circulating tumor cell isolation, culture, and downstream molecular analysis.

Biotechnol Adv 2018 Jul - Aug;36(4):1063-1078. Epub 2018 Mar 17.

Department of Computer & Electrical Engineering and Computer Science, Florida Atlantic University, Boca Raton, FL 33431, USA; Asghar-Lab, Micro and Nanotechnology in Medicine, College of Engineering and Computer Science, Boca Raton, FL 33431, USA; Department of Biological Sciences, Florida Atlantic University, Boca Raton, FL 33431, USA. Electronic address:

Circulating tumor cells (CTCs) are a major contributor of cancer metastases and hold a promising prognostic significance in cancer detection. Performing functional and molecular characterization of CTCs provides an in-depth knowledge about this lethal disease. Researchers are making efforts to design devices and develop assays for enumeration of CTCs with a high capture and detection efficiency from whole blood of cancer patients. Read More

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March 2018
2 Reads

Bio-recycling of metals: Recycling of technical products using biological applications.

Biotechnol Adv 2018 Jul - Aug;36(4):1048-1062. Epub 2018 Mar 16.

Helmholtz-Zentrum Dresden-Rossendorf, Helmholtz Institute Freiberg for Resource Technology, D-09599 Freiberg, Germany.

The increasing demand of different essential metals as a consequence of the development of new technologies, especially in the so called "low carbon technologies" require the development of innovative technologies that enable an economic and environmentally friendly metal recovery from primary and secondary resources. There is serious concern that the demand of some critical elements might exceed the present supply within a few years, thus necessitating the development of novel strategies and technologies to meet the requirements of industry and society. Besides an improvement of exploitation and processing of ores, the more urgent issue of recycling of strategic metals has to be enforced. Read More

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March 2018
2 Reads

Diversity and assembly patterns of activated sludge microbial communities: A review.

Biotechnol Adv 2018 Jul - Aug;36(4):1038-1047. Epub 2018 Mar 15.

Environmental Simulation and Pollution Control State Key Joint Laboratory, School of Environment, Tsinghua University, 100084 Beijing, PR China.

Understanding diversity and assembly patterns of microbial communities in activated sludge (AS) is pivotal for addressing fundamental ecological questions and wastewater treatment engineering. Recent applications of molecular methods especially high-throughput sequencing (HTS) have led to the explosion of information about AS community diversity, including the identification of uncultured taxa, and characterization of low-abundance but environmentally important populations such as antibiotic resistant bacteria and pathogens. Those progresses have facilitated the leverage of ecological theories in describing AS community assembly. Read More

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March 2018
2 Reads

Natural products as modulators of the nuclear receptors and metabolic sensors LXR, FXR and RXR.

Biotechnol Adv 2018 Mar 13. Epub 2018 Mar 13.

University of Vienna, Department of Pharmacognosy, Althanstrasse 14, 1090 Vienna, Austria.

Nuclear receptors (NRs) represent attractive targets for the treatment of metabolic syndrome-related diseases. In addition, natural products are an interesting pool of potential ligands since they have been refined under evolutionary pressure to interact with proteins or other biological targets. This review aims to briefly summarize current basic knowledge regarding the liver X (LXR) and farnesoid X receptors (FXR) that form permissive heterodimers with retinoid X receptors (RXR). Read More

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March 2018
1 Read

Microfluidic devices for sample preparation and rapid detection of foodborne pathogens.

Biotechnol Adv 2018 Jul - Aug;36(4):1003-1024. Epub 2018 Mar 10.

Department of Micro- and Nanotechnology, Technical University of Denmark, Ørsteds Plads, DK-2800 Kgs Lyngby, Denmark. Electronic address:

Rapid detection of foodborne pathogens at an early stage is imperative for preventing the outbreak of foodborne diseases, known as serious threats to human health. Conventional bacterial culturing methods for foodborne pathogen detection are time consuming, laborious, and with poor pathogen diagnosis competences. This has prompted researchers to call the current status of detection approaches into question and leverage new technologies for superior pathogen sensing outcomes. Read More

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March 2018
2 Reads

Effects of shear stress on microalgae - A review.

Biotechnol Adv 2018 Jul - Aug;36(4):986-1002. Epub 2018 Mar 7.

Department of Chemical and Biological Engineering, University of Ottawa, 161 Louis Pasteur Private, Ottawa, ON K1N 6N5, Canada. Electronic address:

Cultivation of microalgae requires consideration of shear stress, which is generated by operations such as mixing, circulation, aeration and pumping that are designed to facilitate mass and heat transfer as well as light distribution in cultures. Excessive shear stress can cause increased cell mortality, decreased growth rate and cell viability, or even cell lysis. This review examines the sources of shear stress in different cultivation systems, shear stress tolerance of different microalgal species and the physiological factors and environmental conditions that may affect shear sensitivity, and potential approaches to mitigate the detrimental effects of shear stress. Read More

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March 2018
15 Reads

Molecular aspects of sucrose transport and its metabolism to starch during seed development in wheat: A comprehensive review.

Biotechnol Adv 2018 Jul - Aug;36(4):954-967. Epub 2018 Feb 28.

Department of Plant Science, University of Manitoba, 222 Agriculture Building, Winnipeg, Manitoba R3T 2N2, Canada. Electronic address:

Wheat is one of the most important crops globally, and its grain is mainly used for human food, accounting for 20% of the total dietary calories. It is also used as animal feed and as a raw material for a variety of non-food and non-feed industrial products such as a feedstock for the production of bioethanol. Starch is the major constituent of a wheat grain, as a result, it is considered as a critical determinant of wheat yield and quality. Read More

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February 2018
2 Reads

Bacterial components as naturally inspired nano-carriers for drug/gene delivery and immunization: Set the bugs to work?

Biotechnol Adv 2018 Jul - Aug;36(4):968-985. Epub 2018 Feb 28.

Wellman Center for Photomedicine, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA; Department of Dermatology, Harvard Medical School, Boston, MA 02115, USA; Harvard-MIT Division of Health Sciences and Technology, Cambridge, MA 02139, USA. Electronic address:

Drug delivery is a rapidly growing area of research motivated by the nanotechnology revolution, the ideal of personalized medicine, and the desire to reduce the side effects of toxic anti-cancer drugs. Amongst a bewildering array of different nanostructures and nanocarriers, those examples that are fundamentally bio-inspired and derived from natural sources are particularly preferred. Delivery of vaccines is also an active area of research in this field. Read More

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February 2018
3 Reads

Paradigm shift - Metabolic transformation of docosahexaenoic and eicosapentaenoic acids to bioactives exemplify the promise of fatty acid drug discovery.

Biotechnol Adv 2018 Jul - Aug;36(4):935-953. Epub 2018 Feb 28.

Department of Biological Sciences, Indian Institute of Science Education and Research, Bhopal, Madhya Pradesh, India.

Fatty acid drug discovery (FADD) is defined as the identification of novel, specialized bioactive mediators that are derived from fatty acids and have precise pharmacological/therapeutic potential. A number of reports indicate that dietary intake of omega-3 fatty acids and limited intake of omega-6 promotes overall health benefits. In 1929, Burr and Burr indicated the significant role of essential fatty acids for survival and functional health of many organs. Read More

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February 2018
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Metabolic regulation in solventogenic clostridia: regulators, mechanisms and engineering.

Biotechnol Adv 2018 Jul - Aug;36(4):905-914. Epub 2018 Feb 22.

Key Laboratory of Synthetic Biology, CAS Center for Excellence in Molecular Plant Sciences, Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai 200032, China; Jiangsu National Synergetic Innovation Center for Advanced Materials, SICAM, 200 North Zhongshan Road, Nanjing 210009, China. Electronic address:

Solventogenic clostridia, a group of important industrial microorganisms, have exceptional substrate and product diversity, capable of producing a series of two-carbon and even long-chain chemicals and fuels by using various substrates, including sugars, cellulose and hemicellulose, and C1 gases. For the sake of in-depth understanding and engineering these anaerobic microorganisms for broader applications, studies on metabolic regulation of solventogenic clostridia had been extensively carried out during the past ten years, based on the rapid development of various genetic tools. To date, a number of regulators that are essential for cell physiological and metabolic processes have been identified in clostridia, and the relevant mechanisms have also been dissected, providing a wealth of valuable information for metabolic engineering. Read More

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February 2018
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Advances in kinome research of parasitic worms - implications for fundamental research and applied biotechnological outcomes.

Biotechnol Adv 2018 Jul - Aug;36(4):915-934. Epub 2018 Mar 19.

Melbourne Veterinary School, Department of Veterinary Biosciences, Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, Victoria 3010, Australia. Electronic address:

Protein kinases are enzymes that play essential roles in the regulation of many cellular processes. Despite expansions in the fields of genomics, transcriptomics and bioinformatics, there is limited information on the kinase complements (kinomes) of most eukaryotic organisms, including parasitic worms that cause serious diseases of humans and animals. The biological uniqueness of these worms and the draft status of their genomes pose challenges for the identification and classification of protein kinases using established tools. Read More

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March 2018
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The functional genomic studies of resveratrol in respect to its anti-cancer effects.

Biotechnol Adv 2018 Feb 22. Epub 2018 Feb 22.

The Institute of Genetics and Animal Breeding, Polish Academy of Sciences, Postępu 36A, Jastrzębiec, 05-552 Magdalenka, Poland.

Resveratrol has anti-cancer effects in vitro, and hypothetical chemopreventive effects in vivo. Effects are pleiotropic, mediated by changes in expression of many genes and epigenetic reprogramming. Thus, they are well suited for functional genomic studies. Read More

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February 2018
1 Read