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    1589 results match your criteria Biotechnology Advances [Journal]

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    Tailoring cyanobacterial cell factory for improved industrial properties.
    Biotechnol Adv 2018 Jan 10. Epub 2018 Jan 10.
    Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, No. 189 Songling Road, Qingdao, 266101, China; Shandong Provincial Key Laboratory of Synthetic Biology, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, No. 189 Songling Road, Qingdao 266101, China; Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China. Electronic address:
    Photosynthetic biomanufacturing provides a promising solution for sustainable production of biofuels and biochemicals. Cyanobacteria are among the most promising microbial platforms for the construction of photosynthetic cell factories. Metabolic engineering of cyanobacteria has enabled effective photosynthetic synthesis of diverse natural or non-natural metabolites, while commercialization of photosynthetic biomanufacturing is usually restricted by process and economic feasibilities. Read More

    Implementing CRISPR-Cas technologies in conventional and non-conventional yeasts: Current state and future prospects.
    Biotechnol Adv 2018 Jan 10. Epub 2018 Jan 10.
    Department of Computer Science and Applied Mathematics, Weizmann Institute of Science, Rehovot 76100, Israel. Electronic address:
    Within five years, the CRISPR-Cas system has emerged as the dominating tool for genome engineering, while also changing the speed and efficiency of metabolic engineering in conventional (Saccharomyces cerevisiae and Schizosaccharomyces pombe) and non-conventional (Yarrowia lipolytica, Pichia pastoris syn. Komagataella phaffii, Kluyveromyces lactis, Candida albicans and C. glabrata) yeasts. Read More

    Designing improved active peptides for therapeutic approaches against infectious diseases.
    Biotechnol Adv 2018 Jan 9. Epub 2018 Jan 9.
    Instituto de Medicina Molecular, Faculdade de Medicina, Universidade de Lisboa, Avenida Professor Egas Moniz, 1649-028 Lisboa, Portugal. Electronic address:
    Infectious diseases are one of the main causes of human morbidity and mortality. In the last few decades, pathogenic microorganisms' resistance to conventional drugs has been increasing, and it is now pinpointed as a major worldwide health concern. The need to search for new therapeutic options, as well as improved treatment outcomes, has therefore increased significantly, with biologically active peptides representing a new alternative. Read More

    Extracellular fungal polyol lipids: A new class of potential high value lipids.
    Biotechnol Adv 2018 Jan 5. Epub 2018 Jan 5.
    Phaff Yeast Culture Collection, Department of Food Science and Technology, University of California, One Shields Ave, Davis, CA 95616, USA. Electronic address:
    Extracellular fungal glycolipid biosurfactants have attracted attention because productivities can be high, cheap substrates can be used, the molecules are secreted into the medium and the downstream processing is relatively simple. Three classes of extracellular fungal glycolipid biosurfactants have provided most of the scientific advances in this area, namely sophorolipids, mannosylerythritol lipids and cellobioselipids. Polyol lipids, a fourth class of extracellular fungal glycolipid biosurfactants, comprise two groups of molecules: liamocins produced by the yeast-like fungus Aureobasidium pullulans, and polyol esters of fatty acids, produced by some Rhodotorula yeast species. Read More

    Microbial and viral chitinases: Attractive biopesticides for integrated pest management.
    Biotechnol Adv 2018 Jan 3. Epub 2018 Jan 3.
    Department of Biotechnology and Life Sciences, University of Insubria, via JH Dunant 3, Varese 21100, Italy; The Protein Factory Research Center, Politecnico di Milano and University of Insubria, via L Mancinelli 7, Milan 20131, Italy. Electronic address:
    The negative impact of the massive use of synthetic pesticides on the environment and on human health has stimulated the search for environment-friendly practices for controlling plant diseases and pests. Among them, biocontrol, which relies on using beneficial organisms or their products (bioactive molecules and/or hydrolytic enzymes), holds the greatest promise and is considered a pillar of integrated pest management. Chitinases are particularly attractive to this purpose since they have fungicidal, insecticidal, and nematicidal activities. Read More

    In vitro methods for testing antiviral drugs.
    Biotechnol Adv 2017 Dec 29. Epub 2017 Dec 29.
    Department of Biochemistry and Microbiology, University of Chemistry and Technology, Prague 166 28, Czech Republic. Electronic address:
    Despite successful vaccination programs and effective treatments for some viral infections, humans are still losing the battle with viruses. Persisting human pandemics, emerging and re-emerging viruses, and evolution of drug-resistant strains impose continuous search for new antiviral drugs. A combination of detailed information about the molecular organization of viruses and progress in molecular biology and computer technologies has enabled rational antivirals design. Read More

    Axenic cultures for microalgal biotechnology: Establishment, assessment, maintenance, and applications.
    Biotechnol Adv 2017 Dec 29. Epub 2017 Dec 29.
    Cell Factory Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 125 Gwahak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea; Department of Environmental Biotechnology, KRIBB School of Biotechnology, Korea University of Science & Technology (UST), 217 Gajeong-ro, Yuseong-gu, Daejeon 34113, Republic of Korea; Advanced Biomass R&D Center (ABC), 291 Daehak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea. Electronic address:
    The impact of microalgae (including blue-green algae or cyanobacteria) on human life can be both beneficiary and deleterious. While microalgae can be cultivated and used as feedstocks for the production of bioenergy and high value-added products in nutraceuticals, pharmaceuticals, and aquaculture feeds, some microalgae cause harmful algal blooms (HABs) that cause large-scale mortality in aquatic environments around the world. Thus, with the development of microalgal biotechnology and increasing concern about HABs, research on microscopic algae has increased significantly. Read More

    Activation of Nrf2 signaling by natural products-can it alleviate diabetes?
    Biotechnol Adv 2017 Dec 28. Epub 2017 Dec 28.
    University of Vienna, Department of Pharmacognosy, Althanstrasse 14, 1090 Vienna, Austria. Electronic address:
    Type 2 diabetes mellitus (DM) has reached pandemic proportions and effective prevention strategies are wanted. Its onset is accompanied by cellular distress, the nuclear factor erythroid 2-related factor 2 (Nrf2) is a transcription factor boosting cytoprotective responses, and many phytochemicals activate Nrf2 signaling. Thus, Nrf2 activation by natural products could presumably alleviate DM. Read More

    The bioelectronic nose and tongue using olfactory and taste receptors: Analytical tools for food quality and safety assessment.
    Biotechnol Adv 2017 Dec 29. Epub 2017 Dec 29.
    Interdisciplinary Program for Bioengineering, Seoul National University, Seoul 151-742, Republic of Korea; School of Chemical and Biological Engineering, Seoul National University, Seoul 151-742, Republic of Korea. Electronic address:
    Food intake is the primary method for obtaining energy and component materials in the human being. Humans evaluate the quality of food by combining various facets of information, such as an item of food's appearance, smell, taste, and texture in the mouth. Recently, bioelectronic noses and tongues have been reported that use human olfactory and taste receptors as primary recognition elements, and nanoelectronics as secondary signal transducers. Read More

    Decentralised manufacturing of cell and gene therapy products: Learning from other healthcare sectors.
    Biotechnol Adv 2017 Dec 24. Epub 2017 Dec 24.
    Centre for Biological Engineering, Holywell Park, Loughborough University, Loughborough LE11 3TU, UK.
    Decentralised or 'redistributed' manufacturing represents an attractive choice for production of some cell and gene therapies (CGTs), in particular personalised therapies. Decentralised manufacturing splits production into various locations or regions and in doing so, imposes organisational changes on the structure of a company. This confers a significant advantage by democratising supply, creating jobs without geographical restriction to the central hub and allowing a more flexible response to external pressures and demands. Read More

    Enhancing the performance of brewing yeasts.
    Biotechnol Adv 2017 Dec 22. Epub 2017 Dec 22.
    Department of Biotechnology, University of Chemistry and Technology, Prague, Technická 5, 16628 Prague 6, Czech Republic. Electronic address:
    Beer production is one of the oldest known traditional biotechnological processes, but is nowadays facing increasing demands not only for enhanced product quality, but also for improved production economics. Targeted genetic modification of a yeast strain is one way to increase beer quality and to improve the economics of beer production. In this review we will present current knowledge on traditional approaches for improving brewing strains and for rational metabolic engineering. Read More

    Nrf2 as regulator of innate immunity: A molecular Swiss army knife!
    Biotechnol Adv 2017 Dec 22. Epub 2017 Dec 22.
    Applied Biotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran. Electronic address:
    Organisms are constantly exposed to a broad range of pathological and stress-inducing agents, allergens and environmental chemicals that can induce infections, toxicity or other undesirable reactions. Our immune system has evolved over time in order to efficiently respond to these exogenous insults and maintain homeostasis. In particular, the innate immune system acts as primary barrier to prevent the entrance of invasive agents or allergens. Read More

    Polymer nanostructures for bioapplications induced by laser treatment.
    Biotechnol Adv 2017 Dec 16. Epub 2017 Dec 16.
    Department of Solid State Engineering, University of Chemistry and Technology, Technická 5, Prague, Czech Republic. Electronic address:
    Modification of polymer substrates can essentially change the properties of material and thereby it allows their usage in attractive fields of material research. Laser treatment can be successfully applied for change in physico-chemical surface properties and/or for selective change of surface morphology with pattern construction. Three major applications of laser induced structures were described, cytocompatibility control, application as anti-bacterial substrate and plasmonic-based detection system. Read More

    Comparative analysis of high butanol tolerance and production in clostridia.
    Biotechnol Adv 2017 Dec 15. Epub 2017 Dec 15.
    Department of Biomedical Engineering, Brno University of Technology, Technicka 12, CZ 61600, Brno, Czech Republic; Department of Physiology, Masaryk University, Kamenice 5, CZ 625 00, Brno, Czech Republic.

    Augmented reality for personalized nanomedicines.
    Biotechnol Adv 2017 Dec 14. Epub 2017 Dec 14.
    Division of Pharmaceutical Sciences, School of Pharmacy, University of Missouri, Kansas City, MO 64108, USA. Electronic address:
    As our understanding of onset and progress of diseases at the genetic and molecular level rapidly progresses, the potential of advanced technologies, such as 3D-printing, Socially-Assistive Robots (SARs) or augmented reality (AR), that are applied to personalized nanomedicines (PNMs) to alleviate pathological conditions, has become more prominent. Among advanced technologies, AR in particular has the greatest potential to address those challenges and facilitate the translation of PNMs into formidable clinical application of personalized therapy. As AR is about to adapt additional new methods, such as speech, voice recognition, eye tracing and motion tracking, to enable interaction with host response or biological systems in 3-D space, a combination of multiple approaches to accommodate varying environmental conditions, such as public noise and atmosphere brightness, will be explored to improve its therapeutic outcomes in clinical applications. Read More

    Bioconversion of carbon dioxide to methane using hydrogen and hydrogenotrophic methanogens.
    Biotechnol Adv 2017 Dec 14. Epub 2017 Dec 14.
    Institute of Chemical Technology Prague, Department of Water Technology and Environmental Engineering, Technicka 5, CZ 166 28 Prague, Czech Republic.
    Biogas produced from organic wastes contains energetically usable methane and unavoidable amount of carbon dioxide. The exploitation of whole biogas energy is locally limited and utilization of the natural gas transport system requires CO2 removal or its conversion to methane. The biological conversion of CO2 and hydrogen to methane is well known reaction without the demand of high pressure and temperature and is carried out by hydrogenotrophic methanogens. Read More

    Involvement of polyhydroxyalkanoates in stress resistance of microbial cells: Biotechnological consequences and applications.
    Biotechnol Adv 2017 Dec 14. Epub 2017 Dec 14.
    Faculty of Chemistry, Brno University of Technology, Purkynova 118, 612 00 Brno, Czech Republic.
    Polyhydroxyalkanoates (PHA) are polyesters accumulated by numerous prokaryotes as storage materials; they attract attention as "green" alternatives to petrochemical plastics. Recent research has demonstrated that their biological role goes beyong their storage function, since they presence in cytoplasm enhances stress resistance of microorganisms. To address these complex functions, this review summarizes the protective effects of PHA for microrganisms; the involvement of PHA in stress resistance is discussed also from a praxis-oriented perspective. Read More

    Importance of microbial defence systems to bile salts and mechanisms of serum cholesterol reduction.
    Biotechnol Adv 2017 Dec 14. Epub 2017 Dec 14.
    Department of Biochemistry and Microbiology, University of Chemistry and Technology, Technická 5, 166 28 Prague, Czech Republic. Electronic address:
    An important feature of the intestinal microbiota, particularly in the case of administered probiotic microorganisms, is their resistance to conditions in the gastrointestinal tract, particularly tolerance to and growth in the presence of bile salts. Bacteria can use several defence mechanisms against bile, including special transport mechanisms, the synthesis of various types of surface proteins and fatty acids or the production of exopolysaccharides. The ability to enzymatically hydrolyse bile salts occurs in a variety of bacteria. Read More

    Synthetic biology of modular endolysins.
    Biotechnol Adv 2017 Dec 15. Epub 2017 Dec 15.
    Department of Applied Biosciences, Ghent University, Valentijn Vaerwyckweg 1 - Building C, 9000 Gent, Belgium. Electronic address:
    Endolysins and their derivatives have emerged in recent years as a novel class of antibacterials, which have now entered the clinical phases. Their rapid mode-of-action and proteinaceous nature differentiates them from any other class of antibiotics. A key feature of endolysins is their modularity and the opportunities that emerge thereof to customize properties such as specificity, activity, stability and solubility. Read More

    Exosome biogenesis, bioactivities and functions as new delivery systems of natural compounds.
    Biotechnol Adv 2017 Dec 14. Epub 2017 Dec 14.
    Department of Drug Sciences, Medicinal Chemistry and Pharmaceutical Technology Section, University of Pavia, Italy. Electronic address:
    A rapidly growing body of experimental evidence has begun to shed light on the wide ranging molecular mechanisms which modulate intra- and inter-cellular communications. A substantial quantity of the available knowledge has only been uncovered in recent years, and we are learning that donor cells release nanovesicles, known as exosomes, which regulate the cellular behavior of recipient cells following uptake. Based on the impressive capacity of exosomes in delivering their "payload", different therapeutic agents, are currently being tested using this delivery method for more effective therapy. Read More

    Bisphenol A (BPA) and cell signaling pathways.
    Biotechnol Adv 2017 Dec 8. Epub 2017 Dec 8.
    Division of Biopharmaceutics and Pharmacokinetics, National Cerebral and Cardiovascular Center Research Institute, 5-7-1 Fujishiro-dai, Suita, Osaka 565-8565, Japan. Electronic address:
    Bisphenol A (BPA; 4,4'-isopropylidenediphenol) is an endocrine disruptor that is used as a material for the production of phenol resins, polyacrylates, polyesters, epoxy resins, and polycarbonate plastics. Endocrine-disruptive or toxic effects of BPA on living organisms through a number of cell signaling pathways have been reported. BPA induces carcinogenesis, reproductive toxicity, abnormal inflammatory or immune response, and developmental disorders of brain or nervous system through various cell signaling pathways. Read More

    Regulation of autophagy by polyphenols: Paving the road for treatment of neurodegeneration.
    Biotechnol Adv 2017 Dec 6. Epub 2017 Dec 6.
    Applied Biotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran 14359-16471, Iran. Electronic address:
    In the present paper, we will discuss on the importance of autophagy in the central nervous system, and outline the relation between autophagic pathways and the pathogenesis of neurodegenerative disorders. The potential therapeutic benefits of naturally occurring phytochemicals as pharmacological modulators of autophagy will also be addressed. Our findings provide renewed insight on the molecular modes of protection by polyphenols, which is likely to be at least in part mediated not only by their potent antioxidant and anti-inflammatory effects, but also through modulation of autophagic processes to remove the aberrant protein aggregates. Read More

    The importance of fungal pathogens and antifungal coatings in medical device infections.
    Biotechnol Adv 2017 Dec 2. Epub 2017 Dec 2.
    Future Industries Institute, University of South Australia, Mawson Lakes Blvd, Mawson Lakes, Adelaide, SA 5000, Australia; School of Agriculture Food & Wine, The University of Adelaide, Waite Campus, Adelaide, SA 5000, Australia. Electronic address:
    In recent years, increasing evidence has been collated on the contributions of fungal species, particularly Candida, to medical device infections. Fungal species can form biofilms by themselves or by participating in polymicrobial biofilms with bacteria. Thus, there is a clear need for effective preventative measures, such as thin coatings that can be applied onto medical devices to stop the attachment, proliferation, and formation of device-associated biofilms. Read More

    Innovative strategies for intervertebral disc regenerative medicine: From cell therapies to multiscale delivery systems.
    Biotechnol Adv 2017 Dec 1. Epub 2017 Dec 1.
    Inserm, UMR 1229, RMeS, Regenerative Medicine and Skeleton, Université de Nantes, ONIRIS, Nantes, France; Université de Nantes, UFR Odontologie, Nantes F-44042, France; CHU Nantes, PHU 4 OTONN, Nantes, France. Electronic address:
    As our understanding of the physiopathology of intervertebral disc (IVD) degeneration has improved, novel therapeutic strategies have emerged, based on the local injection of cells, bioactive molecules, and nucleic acids. However, with regard to the harsh environment constituted by degenerated IVDs, protecting biologics from in situ degradation while allowing their long-term delivery is a major challenge. Yet, the design of the optimal approach for IVD regeneration is still under debate and only a few papers provide a critical assessment of IVD-specific carriers for local and sustained delivery of biologics. Read More

    Harnessing CRISPR/Cas systems for programmable transcriptional and post-transcriptional regulation.
    Biotechnol Adv 2017 Nov 29. Epub 2017 Nov 29.
    Laboratory for Genome Engineering, King Abdullah University of Science and Technology (KAUST), Building #2, Office 3232, 4700 KAUST, Thuwal 23955-6900, Saudia Arabia. Electronic address:
    Genome editing has enabled broad advances and novel approaches in studies of gene function and structure; now, emerging methods aim to precisely engineer post-transcriptional processes. Developing precise, efficient molecular tools to alter the transcriptome holds great promise for biotechnology and synthetic biology applications. Different approaches have been employed for targeted degradation of RNA species in eukaryotes, but they lack programmability and versatility, thereby limiting their utility for diverse applications. Read More

    Hot spots for the protein engineering of Baeyer-Villiger monooxygenases.
    Biotechnol Adv 2017 Nov 22. Epub 2017 Nov 22.
    Institute of Biochemistry, Dept. of Biotechnology & Enzyme Catalysis, Greifswald University, 17487 Greifswald, Germany.
    Baeyer-Villiger monooxygenases (BVMOs) are versatile biocatalysts for the conversion of ketones to lactones or esters while also being able to efficiently oxidize sulfides to sulfoxides. However, there are limitations for the application of BVMOs in synthesis. In this review we provide an overview of the protein engineering studies aiming at optimizing different properties of BVMOs. Read More

    Stability of aerobic granules during long-term bioreactor operation.
    Biotechnol Adv 2017 Nov 20. Epub 2017 Nov 20.
    iBB - Institute for Bioengineering and Biosciences, Department of Bioengineering, Instituto Superior Técnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-001 Lisboa, Portugal. Electronic address:
    Aerobic granular sludge technology has been extensively studied over the past 20 years and is regarded as the upcoming new standard for biological treatment of domestic and industrial wastewaters. Aerobic granules (AG) are dense, compact, self-immobilized microbial aggregates that allow better sludge-water separation and thereby higher biomass concentrations in the bioreactor than conventional activated sludge aggregates. This brings potential practical advantages in terms of investment cost, energy consumption and footprint. Read More

    αvβ3 and α5β1 integrin-specific ligands: From tumor angiogenesis inhibitors to vascularization promoters in regenerative medicine?
    Biotechnol Adv 2017 Nov 15. Epub 2017 Nov 15.
    Life and Health Sciences Research Institute (ICVS), School of Medicine, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal.; ICVS/3B's, PT Government Associate Laboratory, Braga, Guimarães, Portugal.. Electronic address:
    Integrins are cell adhesion receptors predominantly important during normal and tumor angiogenesis. A sequence present on several extracellular matrix proteins composed of Arg-Gly-Asp (RGD) has attracted attention due to its role in cell adhesion mediated by integrins. The development of ligands that can bind to integrins involved in tumor angiogenesis and brake disease progression has resulted in new investigational drug entities reaching the clinical trial phase in humans. Read More

    Biotechnological potential of novel glycoside hydrolase family 70 enzymes synthesizing α-glucans from starch and sucrose.
    Biotechnol Adv 2017 Nov 10. Epub 2017 Nov 10.
    Microbial Physiology, Groningen Biomolecular Sciences and Biotechnology Institute (GBB), University of Groningen, Nijenborgh 7, 9747 AG Groningen, The Netherlands. Electronic address:
    Transglucosidases belonging to the glycoside hydrolase (GH) family 70 are promising enzymatic tools for the synthesis of α-glucans with defined structures from renewable sucrose and starch substrates. Depending on the GH70 enzyme specificity, α-glucans with different structures and physicochemical properties are produced, which have found diverse (potential) commercial applications, e.g. Read More

    Targeting ncRNAs by plant secondary metabolites: The ncRNAs game in the balance towards malignancy inhibition.
    Biotechnol Adv 2017 Nov 10. Epub 2017 Nov 10.
    MEDFUTURE - Research Center for Advanced Medicine, "Iuliu Hatieganu" University of Medicine and Pharmacy, 23 Marinescu Street, 400337, Romania; Research Center for Functional Genomics, Biomedicine and Translational Medicine, "Iuliu Hatieganu" University of Medicine and Pharmacy, 23 Marinescu Street, 400337, Cluj-Napoca, Romania; Department of Functional Genomics and Experimental Pathology, The Oncology Institute "Prof. Dr. Ion Chiricuta", Republicii 34 Street, 400015, Cluj-Napoca, Romania. Electronic address:
    The current trend of combining state of the art technologies with quondam treatments in order to overcome existing gaps in the clinical area determined an increased interest into polyphenols, common dietary phytochemicals, for the prevention and treatment of chronic diseases, especially cancer. The reemergence of polyphenols in the cancer field is sustained by advanced-omics technologies able to identify coding and non-coding genes and their related signaling pathways modulated by natural compounds. Identification of the structural correspondence between interacting molecules will allow the development of more targeted and informed therapeutic strategies for cancer management. Read More

    Engineering strategies for enhanced production of protein and bio-products in Pichia pastoris: A review.
    Biotechnol Adv 2017 Nov 10. Epub 2017 Nov 10.
    School of Chemical Engineering and Technology, Qingdao University of Science and Technology, Qingdao, China; Department of Chemical and Biological Engineering, University of Ottawa, 161 Louis Pasteur Private, Ottawa, ON K1N 6N5, Canada. Electronic address:
    Pichia pastoris has been recognized as one of the most industrially important hosts for heterologous protein production. Despite its high protein productivity, the optimization of P. pastoris cultivation is still imperative due to strain- and product-specific challenges such as promoter strength, methanol utilization type and oxygen demand. Read More

    Amino acid misincorporation in recombinant proteins.
    Biotechnol Adv 2017 Oct 26. Epub 2017 Oct 26.
    Process Development, Amgen Inc., 1 Amgen Center Drive, Thousand Oaks, CA 91320, United States. Electronic address:
    Proteins provide the molecular basis for cellular structure, catalytic activity, signal transduction, and molecular transport in biological systems. Recombinant protein expression is widely used to prepare and manufacture novel proteins that serve as the foundation of many biopharmaceutical products. However, protein translation bioprocesses are inherently prone to low-level errors. Read More

    Next generation organoids for biomedical research and applications.
    Biotechnol Adv 2017 Oct 19. Epub 2017 Oct 19.
    Yale Stem Cell Center, Department of Genetics, Yale School of Medicine, Yale University, New Haven, CT, United States. Electronic address:
    Organoids are in vitro cultures of miniature fetal or adult organ-like structures. Their potentials for use in tissue and organ replacement, disease modeling, toxicology studies, and drug discovery are tremendous. Currently, major challenges facing human organoid technology include (i) improving the range of cellular heterogeneity for a particular organoid system, (ii) mimicking the native micro- and matrix-environment encountered by cells within organoids, and (iii) developing robust protocols for the in vitro maturation of organoids that remain mostly fetal-like in cultures. Read More

    Potential and limitations of Klebsiella pneumoniae as a microbial cell factory utilizing glycerol as the carbon source.
    Biotechnol Adv 2017 Oct 19. Epub 2017 Oct 19.
    School of Energy and Chemical Engineering, UNIST, 50 UNIST-gil, Ulsan 44919, Republic of Korea. Electronic address:
    Klebsiella pneumoniae is a Gram-negative facultative anaerobe that metabolizes glycerol efficiently under both aerobic and anaerobic conditions. This microbe is considered an outstanding biocatalyst for transforming glycerol into a variety of value-added products. Crude glycerol is a cheap carbon source and can be converted by K. Read More

    Improved strategies for electrochemical 1,4-NAD(P)H2 regeneration: A new era of bioreactors for industrial biocatalysis.
    Biotechnol Adv 2017 Oct 10. Epub 2017 Oct 10.
    Department of Chemical and Biological Engineering, Rensselaer Polytechnic Institute, 110 8th Street, Troy, NY 12180, United States; Department of Biological Sciences, Rensselaer Polytechnic Institute, 110 8th Street, Troy, NY 12180, United States. Electronic address:
    Industrial enzymatic reactions requiring 1,4-NAD(P)H2 to perform redox transformations often require convoluted coupled enzyme regeneration systems to regenerate 1,4-NAD(P)H2 from NAD(P) and recycle the cofactor for as many turnovers as possible. Renewed interest in recycling the cofactor via electrochemical means is motivated by the low cost of performing electrochemical reactions, easy monitoring of the reaction progress, and straightforward product recovery. However, electrochemical cofactor regeneration methods invariably produce adventitious reduced cofactor side products which result in unproductive loss of input NAD(P). Read More

    Recent advances in polysaccharide bio-based flocculants.
    Biotechnol Adv 2017 Oct 7. Epub 2017 Oct 7.
    Department of Chemical Engineering and Applied Chemistry, University of Toronto, 200 College St., Toronto, Ontario M5S 3E5, Canada. Electronic address:
    Natural polysaccharides, derived from biomass feedstocks, marine resources, and microorganisms, have been attracting considerable attention as benign and environmentally friendly substitutes for synthetic polymeric products. Besides many other applications, these biopolymers are rapidly emerging as viable alternatives to harmful synthetic flocculating agents for the removal of contaminants from water and wastewater. In recent years, a great deal of effort has been devoted to improve the production and performance of polysaccharide bio-based flocculants. Read More

    Silk fibroin/hydroxyapatite composites for bone tissue engineering.
    Biotechnol Adv 2017 Oct 7. Epub 2017 Oct 7.
    Department of Biomedical Engineering, Tufts University, 4 Colby St, Medford, MA 02155, USA.
    Silk fibroin (SF) is a natural fibrous polymer with strong potential for many biomedical applications. SF has attracted interest in the field of bone tissue engineering due to its extraordinary characteristics in terms of elasticity, flexibility, biocompatibility and biodegradability. However, low osteogenic capacity has limited applications for SF in the orthopedic arena unless suitably functionalized. Read More

    Heterotrophic cultivation of microalgae for pigment production: A review.
    Biotechnol Adv 2017 Sep 22. Epub 2017 Sep 22.
    Collaborative Innovation Center of Biomass Energy, Henan Agricultural University, Henan Province, Zhengzhou 450002, China; Department of Chemical Engineering, National Taiwan University, Taipei 10617, Taiwan; Department of Chemical Engineering, National Taiwan University of Science and Technology, Taipei 10607, Taiwan. Electronic address:
    Pigments (mainly carotenoids) are important nutraceuticals known for their potent anti-oxidant activities and have been used extensively as high end health supplements. Microalgae are the most promising sources of natural carotenoids and are devoid of the toxic effects associated with synthetic derivatives. Compared to photoautotrophic cultivation, heterotrophic cultivation of microalgae in well-controlled bioreactors for pigments production has attracted much attention for commercial applications due to overcoming the difficulties associated with the supply of CO2 and light, as well as avoiding the contamination problems and land requirements in open autotrophic culture systems. Read More

    Cofactor engineering for more efficient production of chemicals and biofuels.
    Biotechnol Adv 2017 Dec 20;35(8):1032-1039. Epub 2017 Sep 20.
    National Energy R&D Center for Biorefinery, Beijing Key Laboratory of Bioprocess, Beijing University of Chemical Technology, 15th, Beisanhuan East Road, Beijing 100029, PR China. Electronic address:
    Cofactors are involved in numerous intracellular reactions and critically influence redox balance and cellular metabolism. Cofactor engineering can support and promote the biocatalysis process, even help driving thermodynamically unfavorable reactions forwards. To achieve efficient production of chemicals and biofuels, cofactor engineering strategies such as altering cofactor supply or modifying reactants' cofactor preference have been developed to maintain redox balance. Read More

    Current advances of succinate biosynthesis in metabolically engineered Escherichia coli.
    Biotechnol Adv 2017 Dec 20;35(8):1040-1048. Epub 2017 Sep 20.
    Key Laboratory of Fermentation Engineering, Ministry of Education, Hubei Provincial Cooperative Innovation Center of Industrial Fermentation, Hubei Key Laboratory of Industrial Microbiology, Hubei University of Technology, Wuhan 430068, China. Electronic address:
    As an important intermediate feedstock, succinate is termed as one of the 12 bio-based platform chemicals. To improve its fermentative production, various strategies have been developed, but challenges are still ahead for succinate biosynthesis to be cost-competitive. In this article, strategies for succinate production through metabolic engineering of Escherichia coli are critically reviewed, with a focus on engineering by-product formation and CO2 fixation, substrate utilization, reducing power balance, metabolic evolution and transcriptional regulation, which provide insights for the current state of succinate production and perspectives for further research for more efficient and economical production of bio-based succinate. Read More

    Learning from quantitative data to understand central carbon metabolism.
    Biotechnol Adv 2017 Dec 18;35(8):971-980. Epub 2017 Sep 18.
    Department of Bioinformatic Engineering, Graduate School of Information Science and Technology, Osaka University, 1-5 Yamadaoka, Suita, Osaka 565-0871, Japan. Electronic address:
    Quantitative analysis of metabolism has been used to identify driver reactions occurring during cancer development and bottleneck reactions in the metabolic engineering of microorganisms. In this review, we compare the advantages and disadvantages of various metabolic analysis methods. We emphasize that metabolic flux analysis based on material balance is a critical method for quantitative investigations into cell metabolism. Read More

    Formulation, construction and analysis of kinetic models of metabolism: A review of modelling frameworks.
    Biotechnol Adv 2017 Dec 13;35(8):981-1003. Epub 2017 Sep 13.
    Australian Institute for Bioengineering and Nanotechnology (AIBN), The University of Queensland, Brisbane, QLD 4072, Australia; The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kemitorvet, Building 220, DK-2800 Kongens Lyngby, Denmark. Electronic address:
    Kinetic models are critical to predict the dynamic behaviour of metabolic networks. Mechanistic kinetic models for large networks remain uncommon due to the difficulty of fitting their parameters. Recent modelling frameworks promise new ways to overcome this obstacle while retaining predictive capabilities. Read More

    Zika virus structural biology and progress in vaccine development.
    Biotechnol Adv 2017 Sep 12. Epub 2017 Sep 12.
    Institute of Biotechnology, National Tsing Hua University, Hsinchu, Taiwan; Department of Medical Science, National Tsing Hua University, Hsinchu, Taiwan. Electronic address:
    The growing number of zika virus (ZIKV) infections plus a 20-fold increase in neonatal microcephaly in newborns in Brazil have raised alarms in many countries regarding the threat to pregnant women. Instances of microcephaly and central nervous system malformations continue to increase in ZIKV outbreak regions. ZIKV is a small enveloped positive-strand RNA virus belonging to the Flavivirus genus of the Flaviviridae family. Read More

    Taxonomy, ecology and biotechnological applications of thraustochytrids: A review.
    Biotechnol Adv 2017 Sep 11. Epub 2017 Sep 11.
    Institute of Mechanical, Process & Energy Engineering, School of Engineering and Physical Sciences, Heriot-Watt University, Edinburgh EH14 4AS, UK. Electronic address:
    Thraustochytrids were first discovered in 1934, and since the 1960's they have been increasingly studied for their beneficial and deleterious effects. This review aims to provide an enhanced understanding of these protists with a particular emphasis on their taxonomy, ecology and biotechnology applications. Over the years, thraustochytrid taxonomy has improved with the development of modern molecular techniques and new biochemical markers, resulting in the isolation and description of new strains. Read More

    Chassis and key enzymes engineering for monoterpenes production.
    Biotechnol Adv 2017 Dec 6;35(8):1022-1031. Epub 2017 Sep 6.
    Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, PR China; SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin University, Tianjin 300072, PR China. Electronic address:
    Microbial production of monoterpenes is often limited by their cytotoxicity and in vivo conversion. Therefore, alleviating cytotoxicity and reducing conversion by chassis engineering are highly desirable. On the other hand, engineering key enzymes is also critical for improving monoterpenes production through facilitating the biosynthesis process. Read More

    Amino acids production focusing on fermentation technologies - A review.
    Biotechnol Adv 2017 Sep 6. Epub 2017 Sep 6.
    Technical University of Denmark, Department of Environmental Engineering, Building 113, DK-2800 Kgs. Lyngby, Denmark. Electronic address:
    Amino acids are attractive and promising biochemicals with market capacity requirements constantly increasing. Their applicability ranges from animal feed additives, flavour enhancers and ingredients in cosmetic to specialty nutrients in pharmaceutical and medical fields. This review gives an overview of the processes applied for amino acids production and points out the main advantages and disadvantages of each. Read More

    Growth media in anaerobic fermentative processes: The underestimated potential of thermophilic fermentation and anaerobic digestion.
    Biotechnol Adv 2017 Sep 1. Epub 2017 Sep 1.
    Section Sanitary Engineering, Department of Water Management, Delft University of Technology, Stevinweg 1, 2628CN Delft, The Netherlands.
    Fermentation and anaerobic digestion of organic waste and wastewater is broadly studied and applied. Despite widely available results and data for these processes, comparison of the generated results in literature is difficult. Not only due to the used variety of process conditions, but also because of the many different growth media that are used. Read More

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