1,888 results match your criteria Biotechnology Advances [Journal]


Progress in the metabolic engineering of bio-based lactams and their ω-amino acids precursors.

Biotechnol Adv 2020 Jul 10:107587. Epub 2020 Jul 10.

McKetta Department of Chemical Engineering, The University of Texas at Austin, 200 E Dean Keeton St. Stop C0400, Austin, TX 78712, United States; Institute for Cellular and Molecular Biology, The University of Texas at Austin, 2500 Speedway Avenue, Austin, TX 78712, United States. Electronic address:

Lactams, cyclic carboxamide acids, are important building blocks as monomers for the manufacture of polyamides (nylons), with a market of millions of tons per year. Likewise, their non-natural building blocks, straight chain ω-amino acids, also have a wide range of applications as pharmaceuticals, therapeutic agents, and precursors to other platform chemicals. Current industrial lactam production requires petrochemically-derived routes that involve the use of harsh chemicals and reaction conditions. Read More

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Heterologous expression of lytic polysaccharide monooxygenases (LPMOs). A mini-review.

Biotechnol Adv 2020 Jul 4:107583. Epub 2020 Jul 4.

Faculty of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences, Ås 1430, Norway. Electronic address:

Lytic polysaccharide monooxygenases (LPMOs) are relatively new enzymes that have been discovered 10 years ago. LPMOs comprise a diverse group of enzymes which play a pivotal role in the depolymerization of sugar-based biopolymers including cellulose, hemicellulose, chitin, and starch. Their mechanism of action relies on the correct coordination of a copper ion in the active site, which is partly composed of the N-terminal histidine. Read More

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Current trends in the production of biodegradable bioplastics: The case of polyhydroxyalkanoates.

Biotechnol Adv 2020 Jul 2:107582. Epub 2020 Jul 2.

Department of Chemistry, Materials and Chemical Engineering "Giulio Natta", Politecnico di Milano, via Mancinelli 7, 20131 Milano, Italy. Electronic address:

The global pollution caused by plastics and microplastics is stimulating intense research towards more environmentally friendly materials, preserving the remarkable application characteristics of the currently available polymers. Among these, polyhydroxyalkanoates (PHAs) have been hailed as the solution to replace conventional, oil-based plastics. Given their biodegradable nature and mechanical properties, their use can be envisioned in a wide range of applications reducing the environmental footprint. Read More

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Consolidated bioprocessing of raw starch to ethanol by Saccharomyces cerevisiae: Achievements and challenges.

Biotechnol Adv 2020 Jun 25:107579. Epub 2020 Jun 25.

Department of Microbiology, Stellenbosch University, Private Bag X1, Matieland 7602, South Africa. Electronic address:

Recent advances in amylolytic strain engineering for starch-to-ethanol conversion have provided a platform for the development of raw starch consolidated bioprocessing (CBP) technologies. Several proof-of-concept studies identified improved enzyme combinations, alternative feedstocks and novel host strains for evaluation and application under fermentation conditions. However, further research efforts are required before this technology can be scaled up to an industrial level. Read More

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Adaptive defensive mechanism of bioleaching microorganisms under extremely environmental acid stress: Advances and perspectives.

Biotechnol Adv 2020 Jun 23:107580. Epub 2020 Jun 23.

Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, People's Republic of China; Key Laboratory of Carbohydrate Chemistry and Biotechnology (Jiangnan University), Ministry of Education, China. Electronic address:

Bioleaching microorganisms inhabit extremely acidic environments (pH < 3.0) and can be used in the biohydrometallurgical industry. They employ several strategies, such as biofilms formation, mechanical defense, membrane reversal potential, proton efflux, intracellular buffering, and protection, or repair mechanism of biomacromolecules, to maintain their intracellular pH within a narrow range, which is close to neutral, for conducting normal physiological activity. Read More

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Lipase immobilization on ceramic supports: An overview on techniques and materials.

Biotechnol Adv 2020 Jun 23:107581. Epub 2020 Jun 23.

Department of Chemical Engineering and Food Engineering (EQA), Federal University of Santa Catarina (UFSC), Florianópolis 88040-900, Santa Catarina, Brazil.

Enzyme immobilization is a well-known technique that allows the reuse of the biocatalyst and generally improves its stability. These improved characteristics are of fundamental importance to turn industrial biocatalysis into viable and competitive processes. The immobilization of enzymes can occur through several physical or chemical techniques. Read More

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Engineering salt tolerance of photosynthetic cyanobacteria for seawater utilization.

Biotechnol Adv 2020 Jun 15:107578. Epub 2020 Jun 15.

Laboratory of Synthetic Microbiology, School of Chemical Engineering & Technology, Tianjin University, Tianjin 300072, PR China; Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering, Ministry of Education of China, Tianjin 300072, PR China; Collaborative Innovation Center of Chemical Science and Engineering, Tianjin, PR China; Center for Biosafety Research and Strategy, Tianjin University, Tianjin, PR China. Electronic address:

Photosynthetic cyanobacteria are capable of utilizing sunlight and CO as sole energy and carbon sources, respectively. With genetically modified cyanobacteria being used as a promising chassis to produce various biofuels and chemicals in recent years, future large-scale cultivation of cyanobacteria would have to be performed in seawater, since freshwater supplies of the earth are very limiting. However, high concentration of salt is known to inhibit the growth of cyanobacteria. Read More

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Applications of small molecules in modulating productivity and product quality of recombinant proteins produced using cell cultures.

Biotechnol Adv 2020 Jun 12:107577. Epub 2020 Jun 12.

Biologics Development, Global Product Development & Supply, Bristol Myers Squibb Company, New Brunswick, NJ 08903, United States of America.

Mammalian cell cultures have been used extensively for production of recombinant protein therapeutics (monoclonal antibodies, fusion proteins, enzymes, etc.) for decades. Small molecules have been investigated as media supplements to improve process productivity and reduce the cost of goods. Read More

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Exploration of space to achieve scientific breakthroughs.

Biotechnol Adv 2020 Jun 12:107572. Epub 2020 Jun 12.

Department of Biology, Friedrich-Alexander-University Erlangen-Nuremberg, Erlangen, Germany; Space Biology Unlimited SAS, Bordeaux, France. Electronic address:

Living organisms adapt to changing environments using their amazing flexibility to remodel themselves by a process called evolution. Environmental stress causes selective pressure and is associated with genetic and phenotypic shifts for better modifications, maintenance, and functioning of organismal systems. The natural evolution process can be used in complement to rational strain engineering for the development of desired traits or phenotypes as well as for the production of novel biomaterials through the imposition of one or more selective pressures. Read More

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Technologies for biological removal and recovery of nitrogen from wastewater.

Biotechnol Adv 2020 Jun 9:107570. Epub 2020 Jun 9.

The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Lyngby, Denmark. Electronic address:

Water contamination is a growing environmental issue. Several harmful effects on human health and the environment are attributed to nitrogen contamination of water sources. Consequently, many countries have strict regulations on nitrogen compound concentrations in wastewater effluents. Read More

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Biotransformation of dietary phytoestrogens by gut microbes: A review on bidirectional interaction between phytoestrogen metabolism and gut microbiota.

Biotechnol Adv 2020 Jun 9:107576. Epub 2020 Jun 9.

State Key Laboratory of Microbial Technology, Microbial Technology Institute, Shandong University, No.72 Binhai Road, Qingdao 266237, PR China.

Phytoestrogens are a class of plant produced polyphenolic compounds with diphenolic structure, which is similar to 17β-estradiol. These phytoestrogens preferentially bind to estrogen receptors, however, with weak affinity. Recently, many studies have found that these phytoestrogens can be transformed by gut microbiota through novel enzymatic reactions into metabolites with altered bioactivity. Read More

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http://dx.doi.org/10.1016/j.biotechadv.2020.107576DOI Listing

Coenzyme A thioester-mediated carbon chain elongation as a paintbrush to draw colorful chemical compounds.

Biotechnol Adv 2020 Jun 5:107575. Epub 2020 Jun 5.

National Engineering Laboratory for Cereal Fermentation Technology (NELCF), Jiangnan University, 1800 Lihu Road, Wuxi, Jiangsu 214122, China; Jiangsu Provincial Research Center for Bioactive Product Processing Technology, Jiangnan University, 1800 Lihu Road, Wuxi, Jiangsu 214122, China. Electronic address:

The biosynthesis of various useful chemicals from simple substrates using industrial microorganisms is becoming increasingly crucial to address the challenge of dwindling non-renewable resources. As the most common intermediate substrates in organisms, Coenzyme A (CoA) thioesters play a central role in the carbon chain elongation process of their products. As a result, numerous of chemicals can be synthesized by the iterative addition of various CoA thioester extender units at a given CoA thioester primer backbone. Read More

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http://dx.doi.org/10.1016/j.biotechadv.2020.107575DOI Listing

Protein folding and assembly in confined environments: Implications for protein aggregation in hydrogels and tissues.

Biotechnol Adv 2020 Jun 6:107573. Epub 2020 Jun 6.

Department of Chemical, Biochemical and Environmental Engineering, University of Maryland Baltimore County, Eng 314, 1000 Hilltop Circle, Baltimore, MD 21250, USA. Electronic address:

In the biological milieu of a cell, soluble crowding molecules and rigid confined environments strongly influence whether the protein is properly folded, intrinsically disordered proteins assemble into distinct phases, or a denatured or aggregated protein species is favored. Such crowding and confinement factors act to exclude solvent volume from the protein molecules, resulting in an increased local protein concentration and decreased protein entropy. A protein's structure is inherently tied to its function. Read More

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Amidase as a versatile tool in amide-bond cleavage: From molecular features to biotechnological applications.

Biotechnol Adv 2020 Jun 5:107574. Epub 2020 Jun 5.

Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou 310014, People's Republic of China; Engineering Research Center of Bioconversion and Biopurification of Ministry of Education, Zhejiang University of Technology, Hangzhou 310014, People's Republic of China; The National and Local Joint Engineering Research Center for Biomanufacturing of Chiral Chemicals, Zhejiang University of Technology, Hangzhou 310014, People's Republic of China.

Amidases (EC 3. 5. 1. Read More

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Algal metabolites: An inevitable substitute for antibiotics.

Biotechnol Adv 2020 Jun 4:107571. Epub 2020 Jun 4.

Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, Brno CZ-613 00, Czech Republic; Central European Institute of Technology, Brno University of Technology, Purkynova 123, Brno CZ-612 00, Czech Republic. Electronic address:

Antibiotic resistance is rising at a pace that is difficult to cope with; circumvention of this issue requires fast and efficient alternatives to conventional antibiotics. Algae inhabit a wide span of ecosystems, which contributes to their ability to synthesize diverse classes of highly active biogenic metabolites. Here, for the first time, we reviewed all possible algal metabolites with broad spectra antibacterial activity against pathogenic bacteria, including antibiotic-resistant strains, and categorized different metabolites of both freshwater and marine algae, linking them on the basis of their target sites and mechanistic actions along with their probable nanoconjugates. Read More

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Purple phototrophic bacteria for resource recovery: Challenges and opportunities.

Biotechnol Adv 2020 May 26:107567. Epub 2020 May 26.

Advanced Water Management Centre, The University of Queensland, Brisbane, QLD 4072, Australia. Electronic address:

Sustainable development is driving a rapid focus shift in the wastewater and organic waste treatment sectors, from a "removal and disposal" approach towards the recovery and reuse of water, energy and materials (e.g. carbon or nutrients). Read More

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Downstream processing technologies in the biocatalytic production of oligosaccharides.

Biotechnol Adv 2020 May 22:107568. Epub 2020 May 22.

Austrian Centre of Industrial Biotechnology (acib), Krenngasse 37, 8010 Graz, Austria; Institute of Biotechnology and Biochemical Engineering, Graz University of Technology, NAWI Graz, Petersgasse 12, 8010 Graz, Austria. Electronic address:

Oligosaccharides are important ingredients for food and feed products. There has therefore been much interest in the development of biocatalytic processes for their production. Irrespective of the oligosaccharide manufacturing route, that is, bottom-up synthesis or controlled depolymerization of a polysaccharide, isolating the product from the reaction mixture usually presents a considerable challenge. Read More

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Alternative sources and metabolic engineering of Taxol: Advances and future perspectives.

Biotechnol Adv 2020 May 21:107569. Epub 2020 May 21.

Department of Biotechnology, College of Agriculture and Natural Resources, University of Tehran, Iran. Electronic address:

Paclitaxel is one of the strong plant-derived anti-cancer drugs that was first isolated from the Pacific yew. Despite many paclitaxel's clinical successes, the limited accessibility of paclitaxel for clinical trials is recognized as the most important challenge. Thus, researchers are continuously trying to find the innovative ways to meet the community's need for this medicine. Read More

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Glycoside hydrolase family 18 chitinases: The known and the unknown.

Biotechnol Adv 2020 May 18:107553. Epub 2020 May 18.

State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection and Shenzhen Agricultural Genome Research Institute, Chinese Academy of Agricultural Sciences, 2 West Yuanmingyuan Road, Beijing 100193, China; School of Bioengineering, Dalian University of Technology, 2 Linggong Road, Dalian 116024, China. Electronic address:

Glycoside hydrolase family 18 (GH18) chitinases, which catalyze the biodegradation of β-1,4 glycosidic bond in amino polysaccharides via a substrate-assisted retention mechanism, are widely distributed in nature and have diverse functions. Many organisms produce several GH18 chitinases which take part in multiple physiological processes, including tissue degradation and remodeling, nutrition uptake, invasion and pathogenesis as well as immune response regulation. Because of their physiological importance, mounting crystallographic investigations have been conducted for GH18 chitinases, and their inhibitors have also been developed. Read More

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Engineering of primary carbon metabolism in filamentous fungi.

Biotechnol Adv 2020 May 10:107551. Epub 2020 May 10.

Fungal Physiology, Westerdijk Fungal Biodiversity Institute & Fungal Molecular Physiology, Utrecht University, Uppsalalaan 8, Utrecht 3584 CT, The Netherlands. Electronic address:

Filamentous fungi are important industrial cell factories used for the production of a wide range of enzymes and metabolites. Their primary metabolism is a significant source of industrially important compounds, as well as of monomeric building blocks for the production of secondary metabolites and extracellular enzymes. Therefore, large efforts have been made towards the development of suitable strains for the industrial scale production of primary metabolites. Read More

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Genetic engineering of microalgae for enhanced biorefinery capabilities.

Biotechnol Adv 2020 May 10:107554. Epub 2020 May 10.

Department of Chemical Engineering, National Cheng Kung University, Tainan 701, Taiwan; Department of Chemical and Materials Engineering, College of Engineering, Tunghai University, Taichung 407, Taiwan; Center for Nanotechnology, Tunghai University, Taichung 407, Taiwan. Electronic address:

Microalgae-based bioproducts are in limelight because of their promising future, novel characteristics, the current situation of population needs, and rising prices of rapidly depleting energy resources. Algae-based products are considered as clean sustainable energy and food resources. At present, they are not commercialized due to their high production cost and low yield. Read More

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http://dx.doi.org/10.1016/j.biotechadv.2020.107554DOI Listing

Production of plant natural products through engineered Yarrowia lipolytica.

Biotechnol Adv 2020 May 15:107555. Epub 2020 May 15.

Institute for Synthetic Biosystem, Department of Biochemical Engineering, School of Chemistry and Chemical Engineering, Beijing Institute of Technology, Beijing 100081, China. Electronic address:

Plant natural products have broad applications in the pharmaceutical, food, cosmetics and flavor industries. However, the current manufacturing methods for plant natural products mainly rely on plant extraction and chemical synthesis, which are associated with unsustainability and severe environmental problems. The advancement of genetic technology has facilitated the engineering of microbes for producing plant natural products, but their industrial production titers remain low. Read More

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Mammalian cell culture for production of recombinant proteins: A review of the critical steps in their biomanufacturing.

Biotechnol Adv 2020 May 13:107552. Epub 2020 May 13.

Cell Technology Group, National Institute for Bioprocessing, Research and Training (NIBRT), Fosters Avenue, Mount Merrion, Blackrock, Dublin A94 X099, Ireland. Electronic address:

The manufacturing of recombinant protein is traditionally undertaken in mammalian cell culture. Today, speed, cost and safety are the primary considerations for process improvements in both upstream and downstream manufacturing. Leaders in the biopharmaceutical industry are striving for continuous improvements to increase throughput, lower costs and produce safer more efficacious drugs. Read More

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Flavonoids, terpenoids, and polyketide antibiotics: Role of glycosylation and biocatalytic tactics in engineering glycosylation.

Biotechnol Adv 2020 Jul - Aug;41:107550. Epub 2020 May 1.

Department of Chemical Engineering, Kyung Hee University, Yongin-si, Gyeonggi-do 17104, Republic of Korea. Electronic address:

Flavonoids, terpenoids, and polyketides are structurally diverse secondary metabolites used widely as pharmaceuticals and nutraceuticals. Most of these molecules exist in nature as glycosides, in which sugar residues act as a decisive factor in their architectural complexity and bioactivity. Engineering glycosylation through selective trimming or extension of the sugar residues in these molecules is a prerequisite to their commercial production as well to creating novel derivatives with specialized functions. Read More

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http://dx.doi.org/10.1016/j.biotechadv.2020.107550DOI Listing

Bacterial cellulose micro-nano fibres for wound healing applications.

Biotechnol Adv 2020 Jul - Aug;41:107549. Epub 2020 Apr 14.

Department of Mechanical Engineering, University College London, London WC1E 7JE, UK. Electronic address:

Bacterial cellulose (BC) is cellulose produced by a few limited species of bacteria in given conditions. BC has many remarkable properties such as its attractive mechanical properties, water uptake ability and biocompatibility which makes it a very desirable material to be used for wound healing. Inherently due to these important properties, the material is very resistant to easy processing and thus difficult to produce into useful entities. Read More

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April 2020
9.015 Impact Factor

The biomedical and bioengineering potential of protein nanocompartments.

Biotechnol Adv 2020 Jul - Aug;41:107547. Epub 2020 Apr 12.

Alberta RNA Research and Training Institute, Department of Chemistry and Biochemistry, University of Lethbridge, 4401 University Drive West, Lethbridge, AB, Canada; Department of Microbiology, Immunology and Infectious Diseases, Cumming, School of Medicine, University of Calgary, 2500 University Dr. N.W., Calgary, AB T2N 1N4, Canada; Li Ka Shing Institute of Virology and Discovery Lab, Faculty of Medicine & Dentistry, University of Alberta, 6-010 Katz Center for Health Research, Edmonton, AB T6G 2E1, Canada. Electronic address:

Protein nanocompartments (PNCs) are self-assembling biological nanocages that can be harnessed as platforms for a wide range of nanobiotechnology applications. The most widely studied examples of PNCs include virus-like particles, bacterial microcompartments, encapsulin nanocompartments, enzyme-derived nanocages (such as lumazine synthase and the E2 component of the pyruvate dehydrogenase complex), ferritins and ferritin homologues, small heat shock proteins, and vault ribonucleoproteins. Structural PNC shell proteins are stable, biocompatible, and tolerant of both interior and exterior chemical or genetic functionalization for use as vaccines, therapeutic delivery vehicles, medical imaging aids, bioreactors, biological control agents, emulsion stabilizers, or scaffolds for biomimetic materials synthesis. Read More

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Common problems associated with the microbial productions of aromatic compounds and corresponding metabolic engineering strategies.

Biotechnol Adv 2020 Jul - Aug;41:107548. Epub 2020 Apr 11.

Key Laboratory of Biobased Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, No. 135 Songling Road, Qingdao 266101, China. Electronic address:

Recent progress in metabolic engineering and synthetic biology has enabled the production of valuable chemicals in microbial cell factories from renewable feedstocks. High value-added aromatic compounds, most of which are traditionally chemically synthesized from petroleum-derived feedstocks, represent a large class of chemicals with industrial significance. The microbial biosynthesis of aromatic compounds has been studied for decades, and varying yields have been achieved for different aromatic compounds. Read More

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Enzymes to unravel bioproducts architecture.

Biotechnol Adv 2020 Jul - Aug;41:107546. Epub 2020 Apr 8.

INRAE, Université de Reims Champagne Ardenne, FARE, F-51100 Reims, France. Electronic address:

Enzymes are essential and ubiquitous biocatalysts involved in various metabolic pathways and used in many industrial processes. Here, we reframe enzymes not just as biocatalysts transforming bioproducts but also as sensitive probes for exploring the structure and composition of complex bioproducts, like meat tissue, dairy products and plant materials, in both food and non-food bioprocesses. This review details the global strategy and presents the most recent investigations to prepare and use enzymes as relevant probes, with a focus on glycoside-hydrolases involved in plant deconstruction and proteases and lipases involved in food digestion. Read More

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A review of high value-added molecules production by microalgae in light of the classification.

Biotechnol Adv 2020 Jul - Aug;41:107545. Epub 2020 Apr 6.

LGPM, CentraleSupélec, Université Paris-Saclay, Centre Européen de Biotechnologie et de Bioéconomie (CEBB), SFR Condorcet FR CNRS 3417, 3 rue des Rouges Terres 51110 Pomacle, France.

This work reviews applications of high added value molecules produced from microalgae. Older forms of valorization - health food and quality feed, polyunsaturated fatty acids, pigments, carbohydrates - are currently penetrating their markets. They are driven by desirable properties: texturer and dye for food industry, antioxidant for cosmetics and the appetite of the general public for biosourced compounds. Read More

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Advances in engineering UDP-sugar supply for recombinant biosynthesis of glycosides in microbes.

Biotechnol Adv 2020 Jul - Aug;41:107538. Epub 2020 Mar 25.

Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China; SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering, Tianjin 300072, China.

Plant glycosides are of great interest for industries. Glycosylation of plant secondary metabolites can greatly improve their solubility, biological activity, or stability. This allows some plant glycosides to be used as food additives, cosmetic products, health products, antisepsis and anti-cancer drugs. Read More

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Library preparation for next generation sequencing: A review of automation strategies.

Biotechnol Adv 2020 Jul - Aug;41:107537. Epub 2020 Mar 19.

Laboratory for MEMS Applications, Department of Microsystems Engineering IMTEK, University of Freiburg, Germany; Hahn-Schickard, Freiburg, Germany. Electronic address:

Next generation sequencing is in the process of evolving from a technology used for research purposes to one which is applied in clinical diagnostics. Recently introduced high throughput and benchtop instruments offer fully automated sequencing runs at a lower cost per base and faster assay times. In turn, the complex and cumbersome library preparation, starting with isolated nucleic acids and resulting in amplified and barcoded DNA with sequencing adapters, has been identified as a significant bottleneck. Read More

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http://dx.doi.org/10.1016/j.biotechadv.2020.107537DOI Listing

Microalgae as a future food source.

Biotechnol Adv 2020 Jul - Aug;41:107536. Epub 2020 Mar 16.

The California Center for Algae Biotechnology, University of California, San Diego, La Jolla, CA, USA; Division of Biological Sciences, University of California, San Diego, La Jolla, CA, USA. Electronic address:

One of the key challenges that we face in the 21st century is the need to feed an ever-increasing human population with increasingly limited natural resources. Even today it is estimated that roughly 1 out of 9 people in the world are undernourished, of which the most important factor is protein-energy malnutrition. By establishing microalgae as a new food and feed platform, we have the opportunity to increase the supply of these essential products to address global demands in a more efficient and environmentally sustainable way. Read More

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Mosses: Versatile plants for biotechnological applications.

Biotechnol Adv 2020 Jul - Aug;41:107533. Epub 2020 Mar 6.

Centro de Análises Bioquímicas e Proteômicas, Universidade Católica de Brasília, Brasilia, DF, Brazil; Programa de Pós-Graduação em Ciências Genômicas e Biotecnologia, Universidade Católica de Brasília, Brasília, DF, Brazil; Programa de Pós-Graduação em Biologia Animal, Universidade de Brasília, Campus Darcy Ribeiro, Brasília, DF, Brazil. Electronic address:

Mosses have long been recognized as powerful experimental tools for the elucidation of complex processes in plant biology. Recent increases in the availability of sequenced genomes and mutant collections, the establishment of novel technologies for targeted mutagenesis, and the development of viable protocols for large-scale production in bioreactors are now transforming mosses into one of the most versatile tools for biotechnological applications. In the present review, we highlight the astonishing biotechnological potential of mosses and how these plants are being exploited for industrial, pharmaceutical, and environmental applications. Read More

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http://dx.doi.org/10.1016/j.biotechadv.2020.107533DOI Listing
March 2020
9.015 Impact Factor

Consolidated bio-saccharification: Leading lignocellulose bioconversion into the real world.

Biotechnol Adv 2020 May - Jun;40:107535. Epub 2020 Feb 24.

CAS Key Laboratory of Biofuels, Shandong Provincial Key Laboratory of Synthetic Biology, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, Shandong 266101, China; Dalian National Laboratory for Clean Energy, Dalian, China; University of Chinese Academy of Sciences, Chinese Academy of Sciences, Beijing, China. Electronic address:

Lignocellulosic biomass is the most abundant sustainable carbon source on the planet and has enormous potential to substitute fossil resources on the premise of cost-effective conversion. Efforts have been made to develop various lignocellulosic bioconversion strategies to overcome biomass recalcitrance, promote product conversion efficiency and reduce process cost. Consolidated bio-saccharification (CBS), a consolidated bioprocessing (CBP) derived strategy, is herein proposed for lignocellulose bioconversion by integrating enzyme production and hydrolysis steps but separating fermentation from the integrated process. Read More

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http://dx.doi.org/10.1016/j.biotechadv.2020.107535DOI Listing
May 2020
9.015 Impact Factor

The challenge and prospect of mRNA therapeutics landscape.

Biotechnol Adv 2020 May - Jun;40:107534. Epub 2020 Feb 21.

School of Life Science, Advanced Research Institute of Multidisciplinary Science, Key Laboratory of Molecular Medicine and Biotherapy, Institute of Engineering Medicine, Beijing Institute of Technology, Beijing 100081, PR China. Electronic address:

Messenger RNA (mRNA)-based therapeutics hold the potential to cause a major revolution in the pharmaceutical industry because they can be used for precise and individualized therapy, and enable patients to produce therapeutic proteins in their own bodies without struggling with the comprehensive manufacturing issues associated with recombinant proteins. Compared with the current therapeutics, the production of mRNA is much cost-effective, faster and more flexible because it can be easily produced by in vitro transcription, and the process is independent of mRNA sequence. Moreover, mRNA vaccines allow people to develop personalized medications based on sequencing results and/or personalized conditions rapidly. Read More

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Enzymes revolutionize the bioproduction of value-added compounds: From enzyme discovery to special applications.

Biotechnol Adv 2020 May - Jun;40:107520. Epub 2020 Jan 23.

acib - Austrian Centre of Industrial Biotechnology, Petersgasse 14, 8010 Graz, Austria.

Competitive sustainable production in industry demands new and better biocatalysts, optimized bioprocesses and cost-effective product recovery. Our review sheds light on the progress made for the individual steps towards these goals, starting with the discovery of new enzymes and their corresponding genes. The enzymes are subsequently engineered to improve their performance, combined in reaction cascades to expand the reaction scope and integrated in whole cells to provide an optimal environment for the bioconversion. Read More

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http://dx.doi.org/10.1016/j.biotechadv.2020.107520DOI Listing

Molecular farming - The slope of enlightenment.

Biotechnol Adv 2020 May - Jun;40:107519. Epub 2020 Jan 16.

Institute for Molecular Biotechnology, RWTH Aachen University, Worringerweg 1, 52074 Aachen, Germany; Fraunhofer Institute for Molecular Biology and Applied Ecology IME, Forckenbeckstrasse 6, 52074 Aachen, Germany.

Molecular farming can be defined as the use of plants to produce recombinant protein products. The technology is now >30 years old. The early promise of molecular farming was based on three perceived advantages: the low costs of growing plants, the immense scalability of agricultural production, and the inherent safety of plants as hosts for the production of pharmaceuticals. Read More

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http://dx.doi.org/10.1016/j.biotechadv.2020.107519DOI Listing

Controlling biofilms using synthetic biology approaches.

Biotechnol Adv 2020 May - Jun;40:107518. Epub 2020 Jan 15.

Department of Chemical and Biological Engineering, Illinois Institute of Technology, Chicago, IL 60616, USA. Electronic address:

Bacterial biofilms are formed by the complex but ordered regulation of intra- or inter-cellular communication, environmentally responsive gene expression, and secretion of extracellular polymeric substances. Given the robust nature of biofilms due to the non-growing nature of biofilm bacteria and the physical barrier provided by the extracellular matrix, eradicating biofilms is a very difficult task to accomplish with conventional antibiotic or disinfectant treatments. Synthetic biology holds substantial promise for controlling biofilms by improving and expanding existing biological tools, introducing novel functions to the system, and re-conceptualizing gene regulation. Read More

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http://dx.doi.org/10.1016/j.biotechadv.2020.107518DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7125041PMC

Mapping and refactoring pathway control through metabolic and protein engineering: The hexosamine biosynthesis pathway.

Biotechnol Adv 2020 May - Jun;40:107512. Epub 2020 Jan 15.

Center for Synthetic Biology, Ghent University, Coupure Links 653, B-9000 Ghent, Belgium. Electronic address:

Microorganisms possess a plethora of regulatory mechanisms to tightly control the flux through their metabolic network, allowing optimal behaviour in response to environmental conditions. However, these mechanisms typically counteract metabolic engineering efforts to rewire the metabolism with a view to overproduction. Hence, overcoming flux control is key in the development of microbial cell factories, illustrated in this contribution using the strictly controlled hexosamine biosynthesis pathway. Read More

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http://dx.doi.org/10.1016/j.biotechadv.2020.107512DOI Listing

Expanding the chemical diversity through microorganisms co-culture: Current status and outlook.

Biotechnol Adv 2020 May - Jun;40:107521. Epub 2020 Jan 15.

Groupe Mer, Molécules, Santé-EA 2160, Faculté des Sciences pharmaceutiques et biologiques, Université de Nantes, 9 rue Bias, BP 53508, F-44035 Nantes Cedex 01, France. Electronic address:

Natural products (NPs) are considered as a cornerstone for the generation of bioactive leads in drug discovery programs. However, one of the major limitations of NP drug discovery program is "rediscovery" of known compounds, thereby hindering the rate of drug discovery efficiency. Therefore, in recent years, to overcome these limitations, a great deal of attention has been drawn towards understanding the role of microorganisms' co-culture in inducing novel chemical entities. Read More

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May 2020
9.015 Impact Factor

Cyanobacterial sigma factors: Current and future applications for biotechnological advances.

Biotechnol Adv 2020 May - Jun;40:107517. Epub 2020 Jan 13.

Centre Algatech, Institute of Microbiology of the Czech Academy of Sciences, Třeboň 37981, Czech Republic; Faculty of Science, University of South Bohemia, České Budějovice 37001, Czech Republic.

A sigma (σ) factor is a constituent of bacterial RNA polymerase that guides the holoenzyme to promoter sequences and initiates transcription. In addition to a primary housekeeping σ factor, bacteria contain a number of alternative σ factors which recognize a specific set of promoters. By replacing the primary σ factor with alternative variants, the cell controls transcription of the whole sets of genes, typically to acclimate to changes in the environment. Read More

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http://dx.doi.org/10.1016/j.biotechadv.2020.107517DOI Listing

Whole-cell biocatalysis using cytochrome P450 monooxygenases for biotransformation of sustainable bioresources (fatty acids, fatty alkanes, and aromatic amino acids).

Biotechnol Adv 2020 May - Jun;40:107504. Epub 2020 Jan 8.

Department of Environmental Engineering, College of Engineering, Ajou University, Suwon, Gyeonggi-do, South Korea. Electronic address:

Cytochrome P450s (CYPs) are heme-thiolated enzymes that catalyze the oxidation of CH bonds in a regio and stereoselective manner. Activation of the non-activated carbon atom can be further enhanced by multistep chemo-enzymatic reactions; moreover, several useful chemicals can be synthesized to provide alternative organic synthesis routes. Given their versatile functionality, CYPs show promise in a number of biotechnological fields. Read More

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http://dx.doi.org/10.1016/j.biotechadv.2020.107504DOI Listing

Molecular priming as an approach to induce tolerance against abiotic and oxidative stresses in crop plants.

Biotechnol Adv 2020 May - Jun;40:107503. Epub 2019 Dec 31.

Department of Molecular Stress Physiology, Center of Plant Systems Biology and Biotechnology, Plovdiv 4000, Bulgaria; Department of Plant Physiology and Molecular Biology, University of Plovdiv, Plovdiv 4000, Bulgaria. Electronic address:

Abiotic stresses, including drought, salinity, extreme temperature, and pollutants, are the main cause of crop losses worldwide. Novel climate-adapted crops and stress tolerance-enhancing compounds are increasingly needed to counteract the negative effects of unfavorable stressful environments. A number of natural products and synthetic chemicals can protect model and crop plants against abiotic stresses through induction of molecular and physiological defense mechanisms, a process known as molecular priming. Read More

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http://dx.doi.org/10.1016/j.biotechadv.2019.107503DOI Listing

The approved gene therapy drugs worldwide: from 1998 to 2019.

Biotechnol Adv 2020 May - Jun;40:107502. Epub 2019 Dec 27.

Department of Pharmacy, State Key Laboratory of Biotherapy and Cancer Center, National Clinical Research Center for Geriatrics, West China Hospital, Sichuan University, and Collaborative Innovation Center of Biotherapy, Chengdu, Sichuan 610041, China.

With the improvement of gene vectors, the rise of chimeric antigen receptor T cell immunotherapy and breakthroughs in the genome editing technology, gene therapy had once again returned to the central stage of disease treatment. It had brought new choices to clinical therapy of diseases such as tumors and genetic diseases, and had changed the status quo of treatment for monogenic disorders and diffuse large B-cell lymphoma. Until August 2019, 22 gene medicines had been approved by the drug regulatory agencies from various countries, but there were few relevant reviews of combing these drugs systematically. Read More

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http://dx.doi.org/10.1016/j.biotechadv.2019.107502DOI Listing

Fungal potential for the degradation of petroleum-based polymers: An overview of macro- and microplastics biodegradation.

Authors:
Carmen Sánchez

Biotechnol Adv 2020 May - Jun;40:107501. Epub 2019 Dec 20.

Laboratory of Biotechnology, Research Centre for Biological Sciences, Universidad Autónoma de Tlaxcala, Ixtacuixtla, C.P. 90120 Tlaxcala, Mexico. Electronic address:

Petroleum-based plastic materials as pollutants raise concerns because of their impact on the global ecosystem and on animal and human health. There is an urgent need to remove plastic waste from the environment to overcome the environmental crisis of plastic pollution. This review describes the natural and unique ability of fungi to invade substrates by using enzymes that have the capacity to detoxify pollutants and are able to act on nonspecific substrates, the fungal ability to produce hydrophobins for surface coating to attach hyphae to hydrophobic substrates, and hyphal ability to penetrate three dimensional substrates. Read More

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http://dx.doi.org/10.1016/j.biotechadv.2019.107501DOI Listing

Towards a new avenue for producing therapeutic proteins: Microalgae as a tempting green biofactory.

Biotechnol Adv 2020 May - Jun;40:107499. Epub 2019 Dec 18.

Research Center for Pharmaceutical Nanotechnology, Biomedicine Institute, Tabriz University of Medical Sciences, Tabriz, Iran; Department of Pharmaceutics, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran. Electronic address:

Most of the recent approved therapeutic proteins are multi-subunit biologics, which need glycosylation and disulfide bridges for their correct conformation and biological functions. Currently, there exist many protein-based drugs that are mostly produced in the Chinese hamster ovary (CHO) cells. However, this expression system appears to associate with some limitations both in upstream and downstream processing steps, including low growth rate, sensitivity to different stresses and pathogens, and time-consuming purification processes. Read More

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http://dx.doi.org/10.1016/j.biotechadv.2019.107499DOI Listing

Biotechnological potential and applications of microbial consortia.

Biotechnol Adv 2020 May - Jun;40:107500. Epub 2019 Dec 18.

Institute of Process Engineering in Life Sciences, Section II: Technical Biology, Karlsruhe Institute of Technology, Fritz-Haber-Weg 4, 76131 Karlsruhe, Germany.

Recent advances in microbial consortia present a valuable approach for expanding the scope of metabolic engineering. Systems biology enable thorough understanding of diverse physiological processes of cells and their interactions, which in turn offers insights into the optimal design of synthetic microbial consortia. Yet, the study of synthetic microbial consortia is still in early infancy, facing many unknowns and challenges in intercellular communication and construction of stable and controllable microbial consortia systems. Read More

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http://dx.doi.org/10.1016/j.biotechadv.2019.107500DOI Listing

Corrigendum to "The functional genomic studies of resveratrol in respect to its anti-cancer effects" [Biotechnol Adv. 2018 Nov 1;36(6):1699-1708].

Biotechnol Adv 2020 Mar - Apr;39:107482. Epub 2019 Dec 12.

The Institute of Genetics and Animal Breeding, Polish Academy of Sciences, Postępu 36A, Jastrzębiec, 05-552 Magdalenka, Poland.

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http://dx.doi.org/10.1016/j.biotechadv.2019.107482DOI Listing
December 2019

Sperm selection in assisted reproduction: A review of established methods and cutting-edge possibilities.

Biotechnol Adv 2020 May - Jun;40:107498. Epub 2019 Dec 11.

Department of Mathematics and Physics E. de Giorgi, University of Salento, Via per Arnesano, 73100 Lecce, Italy; Institute of Nanotechnology, CNR NANOTEC, Via per Monteroni, 73100 Lecce, Italy.

Male infertility often involves idiopathic or unknown causes, leading to an increasing demand for assisted reproduction technologies (ART). Conventional sperm sorting techniques rely on centrifugation steps that are known to cause oxidative stress and consequently damage cells. Alternative novel techniques have been introduced but offer disadvantages that need to be overcome. Read More

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http://dx.doi.org/10.1016/j.biotechadv.2019.107498DOI Listing

Cellular engineering strategies toward sustainable omega-3 long chain polyunsaturated fatty acids production: State of the art and perspectives.

Biotechnol Adv 2020 May - Jun;40:107497. Epub 2019 Dec 9.

Laboratory of Synthetic Microbiology, School of Chemical Engineering & Technology, Tianjin University, Tianjin 300072, PR China; Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering, Ministry of Education of China, Tianjin 300072, PR China; Collaborative Innovation Center of Chemical Science and Engineering, Tianjin, PR China; Center for Biosafety Research and Strategy, Tianjin University, Tianjin, PR China. Electronic address:

Long-chain polyunsaturated fatty acids (LC-PUFAs) especially ω-3 fatty acids provide significant health benefits for human beings. However, ω-3 LC-PUFAs cannot be synthesized de novo in mammals. Traditionally, ω-3 LC-PUFAs are extracted from marine fish, and their production depends on sea fishing, which has not met ever-increasing global demand. Read More

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http://dx.doi.org/10.1016/j.biotechadv.2019.107497DOI Listing