426,639 results match your criteria Biotechnology & Genetic Engineering Reviews[Journal]


Blue and UV-B light synergistically induce anthocyanin accumulation by co-activating nitrate reductase gene expression in Anthocyanin fruit (Aft) tomato.

Plant Biol (Stuttg) 2020 Jun 3. Epub 2020 Jun 3.

Key Laboratory of Saline-alkali Vegetation Ecology Restoration, (Northeast Forestry University), Ministry of Education, Harbin, 150040, China.

The tomato accession LA1996, which carries a dominant allele of Anthocyanin fruit (Aft) locus, accumulates anthocyanins in the peel of fruits under the sunlight. The involvement of blue, UV-A, UV-B and the combination of these lights on the anthocyanin accumulation and the molecular mechanism of their regulation was investigated in LA1996. The most effective treatment for inducing anthocyanin biosynthesis in Aft fruits was co-irradiation of blue and UV-B (blue + UV-B). Read More

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http://dx.doi.org/10.1111/plb.13141DOI Listing

SlBL4 regulates chlorophyll accumulation, chloroplast development and cell wall metabolism in tomato fruit.

J Exp Bot 2020 Jun 3. Epub 2020 Jun 3.

Key Laboratory of Plant Hormones and Development Regulation of Chongqing, School of Life Sciences, Chongqing University, Chongqing, China.

Tomato is a model for studying the climate for fruit development and ripening. Down-regulation of a tomato bell-like homeodomain 4 (SlBL4) resulted in a slightly darker green fruit phenotype and increased accumulation of starch, fructose and glucose. Chlorophyll content analysis and TEM observation confirmed these phenotypes, indicating that SlBL4 was involved in the chlorophyll accumulation and chloroplast formation in tomato. Read More

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http://dx.doi.org/10.1093/jxb/eraa272DOI Listing

Temperature-programmable and enzymatically solidifiable gelatin-based bioinks enable facile extrusion bioprinting.

Biofabrication 2020 Jun 3. Epub 2020 Jun 3.

Center for Human Tissue and Organs Degeneration, Institute of Biomedicine and Biotechnology, Shenzhen Institutes of Advanced Technology Chinese Academy of Sciences, Shenzhen, CHINA.

The development of exceptional bioinks with excellent printability, high fidelity, and excellent cell viability maintenance for extrusion bioprinting remains a major challenge. Gelatin is an ideal candidate bioink due to its biocompatibility, biodegradability, and non-immunogenicity. However, its inherently low viscosity and unstable physical gelation under physiological conditions make it unsuitable for direct extrusion bioprinting of tissue-like gelatin constructs with high fidelity. Read More

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http://dx.doi.org/10.1088/1758-5090/ab9906DOI Listing

Structural extracellular polymeric substances determine the difference in digestibility between waste activated sludge and aerobic granules.

Water Res 2020 May 16;181:115924. Epub 2020 May 16.

Section Sanitary Engineering, Department of Water Management, Faculty of Civil Engineering and Geosciences, Delft University of Technology, Stevinweg 1, 2628, CN, Delft, the Netherlands.

Aerobic granular sludge (AGS) technology is an alternative to conventional activated sludge to reduce the process footprint and energy consumption. Strategies for the efficient management of its produced biomass, that is grown in a granular morphology as well, need further development. Anaerobic digestion (AD) is commonly applied in waste activated sludge (WAS) treatment and is a potential option also for produced AGS treatment. Read More

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http://dx.doi.org/10.1016/j.watres.2020.115924DOI Listing

Multiple HPV infections among men who have sex with men engaged in anal cancer screening in Abuja, Nigeria.

Papillomavirus Res 2020 May 31:100200. Epub 2020 May 31.

University of Maryland Marlene and Stewart Greenebaum Cancer Center, University of Maryland School of Medicine, Baltimore, MD, USA.

Background: Anal precancers and cancers can be detected during screening with high-resolution anoscopy (HRA). The sensitivity of HRA depends on the burden and duration of human papillomavirus (HPV) among those screened as well as anoscopist proficiency, which is highly correlated with prior screening experience. Our objective was to compare the identification and type of HPV and the likelihood of HRA-detected precancer for men who have sex with men (MSM) undergoing their first HRA-screening in Nigeria. Read More

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http://dx.doi.org/10.1016/j.pvr.2020.100200DOI Listing

Combined biochar and nitrogen application stimulates enzyme activity and root plasticity.

Sci Total Environ 2020 May 14;735:139393. Epub 2020 May 14.

Department of Environmental Chemistry, University of Kassel, Nordbahnhofstraße 1a, 37213 Witzenhausen, Germany.

Biochar (BC) and nitrogen (N) fertilizers are frequently applied to improve soil properties and increase crop productivity. Nonetheless, our mechanistic understanding of plant-soil interactions under single or combined application of BC and N remains incomplete. For the first time, we applied a split-root system to evaluate how BC or N contributes to the changes in soil enzyme activities, N and phosphorus (P) cycling as well as root plasticity. Read More

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http://dx.doi.org/10.1016/j.scitotenv.2020.139393DOI Listing

Quality characteristics of white wine: The short- and long-term impact of high power ultrasound processing.

Ultrason Sonochem 2020 May 29;68:105194. Epub 2020 May 29.

University of Zagreb, Faculty of Food Technology and Biotechnology, Pierottijeva 6, 10000 Zagreb, Croatia.

This research aimed to analyze the effects of ultrasound on the quality characteristics of white wine when processed by two different systems, i.e., ultrasonic bath and ultrasonic probe. Read More

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http://dx.doi.org/10.1016/j.ultsonch.2020.105194DOI Listing

Synthetic biology for protein-based materials.

Curr Opin Biotechnol 2020 May 31;65:197-204. Epub 2020 May 31.

State Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic & Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, 800 Dong Chuan Road, Shanghai, 200240, People's Republic of China. Electronic address:

Recombinant protein polymers that mimic the structures and functions of natural proteins and those tailor-designed with new properties provide a family of uniquely tunable and functional materials. However, the diversity of genetically engineered protein polymers is still limited. As a powerful engine for the creation of new biological devices and systems, synthetic biology is promising to tackle the challenges that exist in conventional studies on protein polymers. Read More

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http://dx.doi.org/10.1016/j.copbio.2020.04.004DOI Listing

Metabolic engineering for glycoglycerolipids production in E. coli: Tuning phosphatidic acid and UDP-glucose pathways.

Metab Eng 2020 May 31. Epub 2020 May 31.

Laboratory of Biochemistry, Institut Químic de Sarrià, University Ramon Llull, Via Augusta 350, E-08017, Barcelona, Spain. Electronic address:

Glycolipids are target molecules in biotechnology and biomedicine as biosurfactants, biomaterials and bioactive molecules. An engineered E. coli strain for the production of glycoglycerolipids (GGL) used the MG517 glycolipid synthase from M. Read More

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http://dx.doi.org/10.1016/j.ymben.2020.05.010DOI Listing

Geranium koreanum, a medicinal plant Geranii Herba, ameliorate the gastric mucosa injury in gastritis-induced mice.

J Ethnopharmacol 2020 May 31:113041. Epub 2020 May 31.

Department of Crop Science & Biotechnology, Chonbuk National University, Jeonju, 54896, Republic of Korea. Electronic address:

Ethnopharmacological Relevance: Geranii Herba, the traditional medicinal plants Korean and northeast China, has been used in the healing of a variety of gastrointestinal inflammation disorders. Geranium koreanum is a congeneric origin plant of Geranii Herba that can be used as medicinal plants with Geranium thunbergii, Geranium sibiricum, Geranium carolinianum, etc. However, the research of the biological active about Geranium koreanum is not enough yet. Read More

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http://dx.doi.org/10.1016/j.jep.2020.113041DOI Listing

The antimicrobial peptide tilapia piscidin 3 induces mitochondria-modulated intrinsic apoptosis of osteosarcoma cells.

Biochem Pharmacol 2020 May 31:114064. Epub 2020 May 31.

Department of Marine Biotechnology and Resources, National Sun Yat-sen University, Kaohsiung, 80424, Taiwan; Center for Neuroscience, National Sun Yat-sen University, Kaohsiung, 80424, Taiwan. Electronic address:

Osteosarcoma (OS) is the most common solid tumor of the bone that most often affects adolescents. The introduction of chemotherapy for the treatment of OS has largely improved the survival rates of patients with localized tumors. However, the 5-year survival rate of OS patients with relapsed or metastatic disease is only 10 to 20%. Read More

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http://dx.doi.org/10.1016/j.bcp.2020.114064DOI Listing

Functional Divergence of the Arabidopsis Florigen-Interacting bZIP Transcription Factors FD and FDP.

Cell Rep 2020 Jun;31(9):107717

Max Planck Institute for Plant Breeding Research, Carl-von-Linné-Weg 10, 50829 Cologne, Germany. Electronic address:

Flowering of many plant species depends on interactions between basic leucine zipper (bZIP) transcription factors and systemically transported florigen proteins. Members of the genus Arabidopsis contain two of these bZIPs, FD and FDP, which we show have largely complementary expression patterns in shoot apices before and during flowering. CRISPR-Cas9-induced null mutants for FDP flower slightly earlier than wild-type, whereas fd mutants are late flowering. Read More

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http://dx.doi.org/10.1016/j.celrep.2020.107717DOI Listing

Unipept CLI 2.0: adding support for visualisations and functional annotations.

Bioinformatics 2020 Jun 3. Epub 2020 Jun 3.

Department of Applied Mathematics, Computer Science and Statistics, Ghent University, Ghent, Belgium.

Summary: Unipept is an ecosystem of tools developed for fast metaproteomics data-analysis consisting of a web application, a set of web services (API) and a command-line tool (CLI). After the successful introduction of version 4 of the Unipept web application, we here introduce version 2.0 of the API and CLI. Read More

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http://dx.doi.org/10.1093/bioinformatics/btaa553DOI Listing

Phenotypic responses of foxtail millet (Setaria italica) genotypes to phosphate supply under greenhouse and natural field conditions.

PLoS One 2020 3;15(6):e0233896. Epub 2020 Jun 3.

Xavier Research Foundation, St. Xavier's College, Palayamkottai, India.

Food insecurity is a looming threat for the burgeoning world population. Phosphorus (P), which is absorbed from soil as inorganic phosphate (Pi), is an essential macronutrient for the growth of all agricultural crops. This study reports phenotype analysis for P responses in natural field and greenhouse conditions, using 54 genotypes of foxtail millet (Setaria italica) representing wide geographic origins. Read More

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0233896PLOS

Transcriptional activities of human elongation factor-1α and cytomegalovirus promoter in transgenic dogs generated by somatic cell nuclear transfer.

PLoS One 2020 3;15(6):e0233784. Epub 2020 Jun 3.

Department of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seongbuk-gu, Seoul, Republic of Korea.

Recent advances in somatic cell nuclear transfer (SCNT) in canines facilitate the production of canine transgenic models. Owing to the importance of stable and strong promoter activity in transgenic animals, we tested human elongation factor 1α (hEF1α) and cytomegalovirus (CMV) promoter sequences in SCNT transgenic dogs. After transfection, transgenic donor fibroblasts with the hEF1α-enhanced green fluorescence protein (EGFP) transgene were successfully isolated using fluorescence-activated cell sorting (FACS). Read More

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0233784PLOS

The common pathophysiologic threads between Asian Indian diabetic's 'Thin Fat Phenotype' and partial lipodystrophy: the peripheral adipose tissue transcriptomic evidences.

Adipocyte 2020 Jan;9(1):253-263

Department of Endocrinology, Sawai Man Singh Medical College and Hospital , Jaipur, India.

T2D is a complex disease with poorly understood mechanisms. In Asian Indians, it is associated with "thin fat" phenotype which resembles with partial lipodystrophy. We hypothesized that disturbed expression of lipodystrophy genes might play a role in T2D pathogenesis. Read More

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http://dx.doi.org/10.1080/21623945.2020.1776082DOI Listing
January 2020

Comprehensive analysis of RNA binding motif protein 3 (RBM3) in non-small cell lung cancer.

Cancer Med 2020 Jun 3. Epub 2020 Jun 3.

Department of Clinical Sciences, Division of Oncology and Pathology, Lund University, Lund, Sweden.

Aims: High expression of the RNA-binding motif protein 3 (RBM3) correlates with improved prognosis in several major types of cancer. The aim of the present study was to examine the prognostic value of RBM3 protein and mRNA expression in non-small cell lung cancer (NSCLC).

Methods And Results: Immunohistochemical expression of RBM3 was evaluated in surgically treated NSCLC from two independent patient populations (n = 213 and n = 306). Read More

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http://dx.doi.org/10.1002/cam4.3149DOI Listing

Elucidation of fungal dye-decolorizing peroxidase (DyP) and ligninolytic enzyme activities in decolorization and mineralization of azo dyes.

J Appl Microbiol 2020 Jun 3. Epub 2020 Jun 3.

Center for Biotechnology, School of Pharmaceutical Sciences, Siksha O Anusandhan (Deemed to be University), Bhubaneswar, 751003, Odisha, India.

Aim: The aim of the study is to investigate the efficiency of Geotrichum candidum in the decolorization and mineralization of synthetic azo dyes.

Methods And Results: It includes screening of enzymes from G. candidum and its optimization, followed by decolorization and mineralization studies. Read More

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http://dx.doi.org/10.1111/jam.14731DOI Listing

Smart textiles biotechnology for electrocardiogram monitoring in horses during exercise on treadmill: validation tests.

Equine Vet J 2020 Jun 3. Epub 2020 Jun 3.

Department of Veterinary Sciences, University of Pisa, viale delle Piagge 2, 56124, Pisa, Italy.

Background: There are several bioengineering solutions aimed at improving human health and welfare. Smart electrodes based on textile substrates have met the growing demand for comfort, reliability, and robustness when acquiring physiological signals.

Objectives: Given the importance of good quality electrocardiograms (ECG) in equine sports medicine, this study focuses on the validation of smart textile electrodes to acquire ECG signals in horses during treadmill exercise. Read More

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http://dx.doi.org/10.1111/evj.13296DOI Listing

Functional characteristics of Svl3 and Pam1 that are required for proper cell wall formation in yeast cells.

Yeast 2020 Jun 3. Epub 2020 Jun 3.

Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, China.

In the budding yeast Saccharomyces cerevisiae, Svl3 and Pam1 proteins work as functional homologues. Loss of their function causes increased levels of chitin deposition in the cell wall and temperature sensitivity, suggesting their involvement in cell wall formation. We found that the N- and C-termini of these proteins have distinctive and critical functions. Read More

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http://dx.doi.org/10.1002/yea.3502DOI Listing

Structural basis for the recognition of transiently structured AU-rich elements by Roquin.

Nucleic Acids Res 2020 Jun 3. Epub 2020 Jun 3.

Institute for Molecular Biosciences, Goethe University Frankfurt and Center for Biomolecular Magnetic Resonance (BMRZ), 60438 Frankfurt, Germany.

Adenylate/uridylate-rich elements (AREs) are the most common cis-regulatory elements in the 3'-untranslated region (UTR) of mRNAs, where they fine-tune turnover by mediating mRNA decay. They increase plasticity and efficacy of mRNA regulation and are recognized by several ARE-specific RNA-binding proteins (RBPs). Typically, AREs are short linear motifs with a high content of complementary A and U nucleotides and often occur in multiple copies. Read More

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http://dx.doi.org/10.1093/nar/gkaa465DOI Listing

Effect of low intensity photobiomodulation associated with norbixin-based poly (hydroxybutyrate) membrane on post-tenotomy tendon repair. In vivo study.

Acta Cir Bras 2020 22;35(3):e202000303. Epub 2020 May 22.

Postgraduate Program in Material Engineering (PPGEM), IFPI, Teresina, PI, Brazil.

Purpose: To evaluate the in vivo response of photobiomodulation therapy associated with norbixin-based poly(hydroxybutyrate) membrane (PHB) in tenotomized calcaneal tendon.

Methods: Thirty rats were randomly allocated to six groups (n=5 each): LED groups (L1, L2 and L3) and membrane + LED groups (ML1, ML2 and ML3). The right calcaneal tendons of all animals were sectioned transversely and were irradiated with LED daily, one hour after surgery every 24 hours, until the day of euthanasia. Read More

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http://dx.doi.org/10.1590/s0102-865020200030000003DOI Listing

Enveloped artificial viral capsids self-assembled from anionic β-annulus peptide and cationic lipid bilayer.

Chem Commun (Camb) 2020 Jun 3. Epub 2020 Jun 3.

Department of Chemistry and Biotechnology, Graduate School of Engineering, Tottori University, Koyama-Minami 4-101, Tottori 680-8552, Japan. and Centre for Research on Green Sustainable Chemistry, Tottori University, Koyama-Minami 4-101, Tottori 680-8552, Japan.

Anionic artificial viral capsids were self-assembled from β-annulus-EE peptide, then complexed with lipid-bilayer-containing cationic lipids via electrostatic interaction to form enveloped artificial viral capsids. The critical aggregation concentration of the enveloped artificial viral capsid was significantly lower than that of the uncomplexed artificial viral capsid, indicating that the lipid bilayer stabilised the capsid structure. Read More

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http://dx.doi.org/10.1039/d0cc02622kDOI Listing

Lactobacillus plantarum KFY04 prevents obesity in mice through the PPAR pathway and alleviates oxidative damage and inflammation.

Food Funct 2020 Jun 3. Epub 2020 Jun 3.

Chongqing Collaborative Innovation Center for Functional Food, Chongqing Engineering Research Center of Functional Food, Chongqing Engineering Laboratory for Research and Development of Functional Food, Chongqing University of Education, Chongqing 400067, P.R. China.

In this study, lactic acid bacterium, Lactobacillus plantarum KFY04, was isolated from Xinjiang yogurt, and it was used to intervene in obese mice maintained on a 45% fat diet, and we compared its effects to those of a commercial strain, LDSB, and l-carnitine. The results showed that the LP-KFY04 intervention mice gained weight more slowly and had lower liver, epididymal adipose, and perirenal adipose tissue indices when compared to the other high-fat groups. Moreover, the LP-KFY04 can reduce the formation of fat vacuoles in the liver, while also reducing adipocyte differentiation and volume, and LP-KFY04 groups had the lowest liver and serum AST, ALT, TG, and TC levels and lowest serum LDL-C and highest HDL-C levels among the groups maintained on a high-fat diet. Read More

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http://dx.doi.org/10.1039/d0fo00519cDOI Listing

Detection of mycobacterial CFP-10 (Rv3874) protein in tuberculosis patients by gold nanoparticle-based real-time immuno-PCR.

Future Microbiol 2020 Jun 3. Epub 2020 Jun 3.

Centre for Biotechnology, Maharshi Dayanand University (MDU), Rohtak 124001, Haryana, India.

Timely and reliable diagnostic test for tuberculosis (TB) is immediately required. Attempts were made to improve the technology and diagnostic potential of real-time immuno-PCR (RT-I-PCR). We designed gold nanoparticle (GNP)-based RT-I-PCR (GNP-RT-I-PCR) assay for the detection of CFP-10 (Rv3874) protein in clinical samples of TB patients. Read More

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http://dx.doi.org/10.2217/fmb-2019-0347DOI Listing

Interleukin 6 polymorphisms as an indicator of COVID-19 severity in humans.

J Biomol Struct Dyn 2020 Jun 3:1-4. Epub 2020 Jun 3.

Department of Biotechnology, Institute of Biotechnology, College of Life and Applied Sciences, Yeungnam University, 280 Daehak-Ro, Gyeongsan, Gyeongbuk 38541, Republic of Korea.

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http://dx.doi.org/10.1080/07391102.2020.1776640DOI Listing

Validation and Disease Risk Assessment of Previously Reported Genome-Wide Genetic Variants Associated with Brugada Syndrome: SADS-TW BrS Registry.

Circ Genom Precis Med 2020 Jun 3. Epub 2020 Jun 3.

Departments of Cardiovascular Medicine (Division of Heart Rhythm Services), Pediatric and Adolescent Medicine (Division of Pediatric Cardiology), and Molecular Pharmacology & Experimental Therapeutics (Windland Smith Rice Sudden Death Genomics Laboratory), Mayo Clinic, Rochester, MN.

- Brugada syndrome (BrS) is an oligogenic arrhythmic disease with increased risk of sudden cardiac arrest (SCA). Several BrS or ECG traits-related single nucleotide polymorphisms (SNPs) were identified through previous genome-wide association studies in Caucasian patients. We aimed to validate these SNPs in BrS patients in the Taiwanese population, assessing the cumulative effect of risk alleles and the BrS polygenic risk score (BrS-PRS) in predicting cardiac events. Read More

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http://dx.doi.org/10.1161/CIRCGEN.119.002797DOI Listing

Biosynthesis of antioxidative enzymes and polyphenolics content in calli cultures of L. in response to auxins and cytokinins.

Artif Cells Nanomed Biotechnol 2020 Dec;48(1):893-902

Center for Biotechnology and Microbiology, University of Swat, Swat, Pakistan.

L. is one of the therapeutic herbs containing various polyphenolics, which is used for multiple medicinal purposes. In this study, plant growth regulators (PGRs)-induced calli cultures from seed-derived leaf explants were exploited for the production of stress enzymes and polyphenolics. Read More

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http://dx.doi.org/10.1080/21691401.2020.1771349DOI Listing
December 2020

Mitochondrial ion channels as targets for cardioprotection.

J Cell Mol Med 2020 Jun 3. Epub 2020 Jun 3.

Department of Biomedical Sciences, University of Padova, Padova, Italy.

Acute myocardial infarction (AMI) and the heart failure (HF) that often result remain the leading causes of death and disability worldwide. As such, new therapeutic targets need to be discovered to protect the myocardium against acute ischaemia/reperfusion (I/R) injury in order to reduce myocardial infarct (MI) size, preserve left ventricular function and prevent the onset of HF. Mitochondrial dysfunction during acute I/R injury is a critical determinant of cell death following AMI, and therefore, ion channels in the inner mitochondrial membrane, which are known to influence cell death and survival, provide potential therapeutic targets for cardioprotection. Read More

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http://dx.doi.org/10.1111/jcmm.15341DOI Listing

Biomaterials functionalized with nanoclusters of integrin- and syndecan-binding ligands improve cell adhesion and mechanosensing under shear flow conditions.

J Biomed Mater Res A 2020 Jun 3. Epub 2020 Jun 3.

Department of Biomedical Engineering, Particulate Fluids Processing Centre, University of Melbourne, Parkville, Victoria, Australia.

We have engineered biomaterials that display nanoclusters of ligands that bind both integrin and syndecan-4 cell receptors. These surfaces regulate cell behaviours under static conditions including adhesion, spreading, actin stress fibre formation, and migration. The syndecan-4 receptors are also critical mediators of cellular mechanotransduction. Read More

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http://dx.doi.org/10.1002/jbm.a.37024DOI Listing

Differentiation, evolution and utilization of natural alleles for cold adaptability at the reproductive stage in rice.

Plant Biotechnol J 2020 Jun 3. Epub 2020 Jun 3.

State Key Laboratory of Agrobiotechnology/Beijing Key Laboratory of Crop Genetic Improvement, College of Agronomy and Biotechnology, China Agricultural University, Beijing, 100193, China.

Genetic studies on cold tolerance at the reproductive stage in rice could lead to significant reductions in yield losses. However, knowledge about the genetic basis and adaptive differentiation, as well as the evolution and utilization of the underlying natural alleles remain limited. Here, 580 rice accessions in two association panels were used to perform genome-wide association study, and 156 loci associated with cold tolerance at the reproductive stage were identified. Read More

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http://dx.doi.org/10.1111/pbi.13424DOI Listing

In-vitro digestion of the bioactives originating from the Lamiaceae family herbal teas: A kinetic and PLS modeling study.

J Food Biochem 2020 Jun 3:e13233. Epub 2020 Jun 3.

Faculty of Food Technology and Biotechnology, Department of Process Engineering, University of Zagreb, Zagreb, Croatia.

The stability of lavender, lemon balm, mint, sage, and thyme teas was investigated using in-vitro simulation of the digestive system. Kinetics of changes in the total polyphenolic content (TPC) and the antioxidant activity during the in-vitro trials were also evaluated. Results showed that TPC of mint teas decreases the fastest. Read More

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http://dx.doi.org/10.1111/jfbc.13233DOI Listing

A homologous overexpression system to study roles of drug transporters in Candida glabrata.

FEMS Yeast Res 2020 Jun 3. Epub 2020 Jun 3.

Yeast Biofuel Group, International Centre for Genetic Engineering and Biotechnology, New Delhi, India.

Considering the relevance of drug transporters belonging to ABC and MFS superfamilies in pathogenic Candida species, there has always been a need to have an overexpression system where these membrane proteins for functional analysis could be expressed in a homologous background. We could address this unmet need by constructing a highly drug-susceptible Candida glabrata strain deleted in seven dominant ABC transporters genes such as CgSNQ2, CgAUS1, CgCDR1, CgPDH1, CgYCF1, CgYBT1, and CgYOR1 and introduced a GOF mutation in transcription factor (TF) CgPDR1 leading to a hyper-activation of CgCDR1 locus. The expression system was validated by overexpressing four GFP tagged ABC (CgCDR1, CgPDH1, CaCDR1, and ScPDR5) and an MFS (CgFLR1) transporters genes facilitated by an engineered expression plasmid to integrate at the CgCDR1 locus. Read More

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http://dx.doi.org/10.1093/femsyr/foaa032DOI Listing

Nanobodies derived from Camelids represent versatile biomolecules for biomedical applications.

Biomater Sci 2020 Jun 3. Epub 2020 Jun 3.

Center for Global Health, School of Public Health, Nanjing Medical University, 211166 Nanjing, China. and Key Laboratory of Antibody Technique of National Health Commission, Nanjing Medical University, Nanjing 211166, China and The Key Laboratory of Modern Toxicology, Ministry of Education, School of Public Health, Nanjing Medical University, 211166 Nanjing, China.

Nanobodies are antigen binding variable domains of heavy-chain antibodies without light-chains, and these biomolecules occur naturally in the serum of Camelidae species. Nanobodies have a compact structure and low molecular weight when compared with antibodies, and are the smallest active antigen-binding fragments. Because of their remarkable stability and manipulable characteristics, nanobodies have been incorporated into biomaterials and used as molecular recognition and tracing agents, drug delivery systems, molecular imaging tools and disease therapeutics. Read More

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http://dx.doi.org/10.1039/d0bm00574fDOI Listing

A point-to-point protein-protein interaction assay reveals the signaling interplays among plant hormones and environmental cues.

Plant Direct 2020 May 25;4(5):e00228. Epub 2020 May 25.

Jiangsu Key Laboratory for Biodiversity and Biotechnology College of Life Sciences Nanjing Normal University Nanjing China.

As sessile organisms, plants must properly coordinate their growth and developmental programs with changes in the environment. The integration of exogenous environmental cues with endogenous plant hormone responses often occurs through physical protein-protein interactions (PPIs). However, a comprehensive PPI network that mediates environmental and hormonal responses has not been established. Read More

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http://dx.doi.org/10.1002/pld3.228DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7247280PMC

Radioprotection of thymine and calf thymus DNA by an azo compound: mechanism of action followed by DPPH radical quenching & ROS depletion in WI 38 lung fibroblast cells.

Heliyon 2020 May 29;6(5):e04036. Epub 2020 May 29.

Department of Chemistry (Inorganic Section), Jadavpur University, Kolkata 700032, India.

Purpose: To explain the observed radio-protection properties of an azo compound, 2-(2-hydroxyphenylazo)-indole-3-acetic acid (HPIA).

Materials And Methods: Mechanism of radioprotection by HPIA was attempted using the stable free radical 2, 2-diphenyl-1-picrylhydrazyl (DPPH) using UV-Vis and electron paramagnetic resonance (EPR) spectroscopy. The radical destroying ability of HPIA was studied by depletion of reactive oxygen species (ROS) in WI 38 lung fibroblast cells. Read More

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http://dx.doi.org/10.1016/j.heliyon.2020.e04036DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7262411PMC

Glutathione dynamics determine the therapeutic efficacy of mesenchymal stem cells for graft-versus-host disease via CREB1-NRF2 pathway.

Sci Adv 2020 Apr 15;6(16):eaba1334. Epub 2020 Apr 15.

Department of Biomedical Sciences, Asan Medical Center, University of Ulsan College of Medicine, Seoul 05505, Korea.

Glutathione (GSH), the most abundant nonprotein thiol functioning as an antioxidant, plays critical roles in maintaining the core functions of mesenchymal stem cells (MSCs), which are used as a cellular immunotherapy for graft-versus-host disease (GVHD). However, the role of GSH dynamics in MSCs remains elusive. Genome-wide gene expression profiling and high-throughput live-cell imaging assays revealed that CREB1 enforced the GSH-recovering capacity (GRC) of MSCs through NRF2 by directly up-regulating NRF2 target genes responsible for GSH synthesis and redox cycling. Read More

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http://dx.doi.org/10.1126/sciadv.aba1334DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7239701PMC

An Epoxide Intermediate in Glycosidase Catalysis.

ACS Cent Sci 2020 May 16;6(5):760-770. Epub 2020 Apr 16.

School of Chemistry and Bio21 Molecular Science and Biotechnology Institute, University of Melbourne, Parkville, Victoria 3010, Australia.

Retaining glycoside hydrolases cleave their substrates through stereochemical retention at the anomeric position. Typically, this involves two-step mechanisms using either an enzymatic nucleophile via a covalent glycosyl enzyme intermediate or neighboring-group participation by a substrate-borne 2-acetamido neighboring group via an oxazoline intermediate; no enzymatic mechanism with participation of the sugar 2-hydroxyl has been reported. Here, we detail structural, computational, and kinetic evidence for neighboring-group participation by a mannose 2-hydroxyl in glycoside hydrolase family 99 -α-1,2-mannanases. Read More

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http://dx.doi.org/10.1021/acscentsci.0c00111DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7256955PMC

A Biological Nanomachine at Work: Watching the Cellulosome Degrade Crystalline Cellulose.

ACS Cent Sci 2020 May 6;6(5):739-746. Epub 2020 May 6.

Institute of Biotechnology and Biochemical Engineering, Graz University of Technology, Petersgasse 10-12/1, 8010 Graz, Austria.

The cellulosome is a supramolecular multienzymatic protein complex that functions as a biological nanomachine of cellulosic biomass degradation. How the megadalton-size cellulosome adapts to a solid substrate is central to its mechanism of action and is also key for its efficient use in bioconversion applications. We report time-lapse visualization of crystalline cellulose degradation by individual cellulosomes from by atomic force microscopy. Read More

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http://dx.doi.org/10.1021/acscentsci.0c00050DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7256933PMC

Leaf tissue specific transcriptome sequence and assembly datasets of Asiatic mangrove Lam.

Data Brief 2020 Aug 22;31:105747. Epub 2020 May 22.

Department of Biotechnology and Microbiology, Kannur University, Thalassery Campus, Palayad P.O., Kannur- 670661, Kerala, India.

Transcriptome data is beneficial to explore molecular mechanisms of extreme adaptations in non- model organisms like mangroves. In this data article, five major datasets and two data sub sets of a salt secreting mangrove, Lam. were described. Read More

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http://dx.doi.org/10.1016/j.dib.2020.105747DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7262405PMC

Complete genome sequence data of MBEL1397 isolated from kimchi.

Data Brief 2020 Aug 22;31:105740. Epub 2020 May 22.

Division of Food Biotechnology and Biosystems Engineering, Kangwon National University, Chuncheon 24341, Republic of Korea.

MBEL1397(=KCTC14037BP) was isolated from kimchi, a traditional Korean fermented food, in Gangwon province, Republic of Korea. MBEL1397 is an acid-tolerant strain with antimicrobial activity and α-glucosidase inhibitory activity, which might be preliminary indications of its probiotic properties. Complete genome sequencing of MBEL1397 was performed using the PacBio RSII platform. Read More

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http://dx.doi.org/10.1016/j.dib.2020.105740DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7260432PMC

Data on metal-chelating, -immobilisation and biosorption properties by CWB2 in dependency on rare earth adaptation.

Data Brief 2020 Aug 21;31:105739. Epub 2020 May 21.

Institute of Biosciences, Environmental Microbiology Group, TU Bergakademie Freiberg, Leipziger Straße 29, 09599 Freiberg, Germany.

Recent studies have shown that the metal adaptation of Actinobacteria offers a rich source of metal inducible environmentally relevant bio-compounds and molecules. These interact through biosorption towards the unique cell walls or via metal chelating activity of metallophors with trace elements, heavy metals and even with lanthanides to overcome limitations and toxic concentrations. Herein, the purpose is to investigate the adaptation potential of CWB2 in dependence of the rare earths and to determine if we can utilize promising metallophore metal affinities for metal separation from aquatic solutions. Read More

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http://dx.doi.org/10.1016/j.dib.2020.105739DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7262544PMC

Illumina and PacBio DNA sequencing data, assembly and annotation of the genome of strain CCAP_4062/1.

Data Brief 2020 Aug 21;31:105729. Epub 2020 May 21.

Laboratoire de Physiologie Cellulaire Végétale, Université Grenoble Alpes, CEA, CNRS, INRAE, IRIG-LPCV, 38054 Grenoble Cedex 9, France.

The complete genome of the thraustochytrid strain CCAP_4062/1 was sequenced using both Illumina Novaseq 6000 and third generation sequencing technology PacBio RSII in order to obtain trustworthy assembly and annotation. The reads from both platforms were combined at multiple levels in order to obtain a reliable assembly, then compared to the ATCC MYA1381™ reference genome. The final assembly was annotated with the help of strain CCAP_4062/1 RNAseq data. Read More

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http://dx.doi.org/10.1016/j.dib.2020.105729DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7262427PMC

The first complete mitochondrial genome data of originating from Malaysia.

Data Brief 2020 Aug 21;31:105721. Epub 2020 May 21.

Department of Biosciences, Faculty of Science, Universiti Teknologi Malaysia, 81310 Johor Bahru, Johor, Malaysia.

The spotted seahorse, population is exponentially decreasing globally due to habitat loss contributed by massive coastal urbanization as well as its large exploitation for Chinese herbal medicine. Genomic data would be highly useful to improve biomonitoring of seahorse populations in Malaysia via the usage of non-invasive approaches such as water environmental DNA. Here we report the first complete mitogenome of two individuals originating from Malaysia, generated using BGISEQ-500RS sequencer. Read More

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http://dx.doi.org/10.1016/j.dib.2020.105721DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7260291PMC

Caspase Dependent and Independent Anti-hematological Malignancy Activity of AMHA1 Attenuated Newcastle Disease Virus.

Int J Mol Cell Med 2019 ;8(3):211-223

Experimental Therapy Department, Iraqi Center for cancer and medical genetics research, Mustansiriyah University, Baghdad, Iraq.

Hematological malignancies remain one of the leading causes of death worldwide despite advances in cancer therapeutics. Newcastle disease virus (NDV) is a member of that elicits considerable interest as an anticancer agent because it can replicate up to 10 000 times faster in human cancer cells than in most normal cancer cells. Several NDV strains reportedly induce the cytolysis of cancerous cell lines. Read More

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http://dx.doi.org/10.22088/IJMCM.BUMS.8.3.211DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7241843PMC
January 2019

Whole Exome Sequencing Revealed a Novel GJB1 Pathogenic Variant and a Rare BSCL2 Mutation in Two Iranian Large Pedigrees with Multiple Affected Cases of Charcot-Marie-Tooth.

Int J Mol Cell Med 2019 ;8(3):169-178

Department of Genetics and Molecular Medicine, School of Medicine, Zanjan University of Medical Sciences (ZUMS), Zanjan, Iran.

Charcot-Marie-Tooth disease (CMT) is the most common hereditary neuropathy of the peripheral nervous system with a wide range of severity and age of onset. CMT patients share similar phenotypes which make it often impossible to identify the disease types based on clinical presentation and electrophysiological studies alone. In recent years, novel genetic diagnostic approaches such as whole exome sequencing (WES) has provided a ground for accurate diagnosis of CMT through identification of the disease-causing mutation(s). Read More

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http://dx.doi.org/10.22088/IJMCM.BUMS.8.3.169DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7241839PMC
January 2019

Biochemical profiling of three indigenous isolates with main focus on fatty acid composition towards potential biotechnological application.

Biotechnol Rep (Amst) 2020 Jun 25;26:e00479. Epub 2020 May 25.

Department of Food Biotechnology, Branch for Northwest & West region, Agricultural Biotehnology Research Institute of Iran, Agricultural Research, Education and Extension Organization (AREEO), Tabriz, Iran.

This study describes the biochemical composition of three isolates, sp. ABRIINW-B1, -G2/1 and -I1 towards the biotechnological potential. sp. Read More

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http://dx.doi.org/10.1016/j.btre.2020.e00479DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7262423PMC

The Link Between Rheumatoid Arthritis and Dementia: A Review.

Cureus 2020 Apr 27;12(4):e7855. Epub 2020 Apr 27.

Internal Medicine, American University of Integrative Sciences School of Medicine, St. Michael, BRB.

Background:  This study explored the relationship between rheumatoid arthritis (RA) and dementia. These two diseases are a significant health burden that affects the older population, although they have also manifested in young people to a smaller extent.

Methodology:  The study entailed a detailed literature review of articles on RA and dementia. Read More

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http://dx.doi.org/10.7759/cureus.7855DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7255531PMC

COVID-19 Virulence in Aged Patients Might Be Impacted by the Host Cellular MicroRNAs Abundance/Profile.

Aging Dis 2020 May 9;11(3):509-522. Epub 2020 May 9.

1Department of Medicine, Augusta University, Augusta, GA, USA.

The World health organization (WHO) declared Coronavirus disease 2019 (COVID-19) a global pandemic and a severe public health crisis. Drastic measures to combat COVID-19 are warranted due to its contagiousness and higher mortality rates, specifically in the aged patient population. At the current stage, due to the lack of effective treatment strategies for COVID-19 innovative approaches need to be considered. Read More

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http://dx.doi.org/10.14336/AD.2020.0428DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7220294PMC

COVID-19 in India: Are Biological and Environmental Factors Helping to Stem the Incidence and Severity?

Aging Dis 2020 May 9;11(3):480-488. Epub 2020 May 9.

17Department of Biochemistry and Central Research Cell, MM Institute of Medical Sciences & Research, Maharishi Markandeshwar (deemed to be) University, Mullana, Haryana, India.

The ongoing Corona virus (COVID-19) pandemic has witnessed global political responses of unimaginable proportions. Many nations have implemented lockdowns that involve mandating citizens not to leave their residences for non-essential work. The Indian government has taken appropriate and commendable steps to curtail the community spread of COVID-19. Read More

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http://dx.doi.org/10.14336/AD.2020.0402DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7220291PMC