431,246 results match your criteria Biotechnology & Biotechnological Equipment[Journal]


Metal induced non-metallothionein protein in earthworm: A new pathway for cadmium detoxification in chloragogenous tissue.

J Hazard Mater 2020 Jul 1;401:123357. Epub 2020 Jul 1.

Soil and Agro Bio-engineering Lab, Department of Environmental Science, Tezpur University, Tezpur, 784028, India. Electronic address:

Earthworms neutralize toxic metals by a small (∼13 kDa) cysteine rich metal binding protein, metallothionein (MT). Although the rate of metal accumulation and MT expression does not correlate well, the reason behind such inconsistency has not yet been deciphered. The present investigation clearly demonstrates that expression of some non-MT metal induced proteins is responsible for such incongruity. Read More

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http://dx.doi.org/10.1016/j.jhazmat.2020.123357DOI Listing

Structure guided design and synthesis of furyl thiazolidinedione derivatives as inhibitors of GLUT 1 and GLUT 4, and evaluation of their anti-leukemic potential.

Eur J Med Chem 2020 Jul 2;202:112603. Epub 2020 Jul 2.

Department of Pharmaceutical Chemistry, Bharati Vidyapeeth's College of Pharmacy, Navi Mumbai, Maharashtra, India. Electronic address:

Cancer cells increase their glucose uptake and glycolytic activity to meet the high energy requirements of proliferation. Glucose transporters (GLUTs), which facilitate the transport of glucose and related hexoses across the cell membrane, play a vital role in tumor cell survival and are overexpressed in various cancers. GLUT1, the most overexpressed GLUT in many cancers, is emerging as a promising anti-cancer target. Read More

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http://dx.doi.org/10.1016/j.ejmech.2020.112603DOI Listing

Synergistic infection of Ichthyophthirius multifiliis and Francisella noatunensis subsp. orientalis in hybrid red tilapia (Oreochromis sp.).

Microb Pathog 2020 Jul 4:104369. Epub 2020 Jul 4.

Fish Infectious Diseases Research Unit (FID RU), Department of Veterinary Microbiology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand. Electronic address:

Francisella noatunensis subsp. orientalis (Fno) and Ichthyophthirius multifiliis (Ich) are deadly infectious pathogens in farmed tilapia, particularly during cold season when the water temperature drops to under 25 °C. We hypothesized that infection of the ectoparasite Ich might enhance susceptibility of hybrid red tilapia (Oreochromis sp. Read More

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http://dx.doi.org/10.1016/j.micpath.2020.104369DOI Listing

Isolation, identification and characterization of propionic acid bacteria associated with autistic spectrum disorder.

Microb Pathog 2020 Jul 4:104371. Epub 2020 Jul 4.

Institute of Biotechnology, Zhejiang University, Hangzhou, 310058, China. Electronic address:

Autism spectrum disorder (ASD) seriously affects children's health. Recently, propionic acid (PA) has been reported to play a significant role in the formation of ASD. In this study, we investigated the community structure of PA-related bacteria in healthy and ASD children by isolation and culture, while a group of representative PA-related bacteria were identified and characterized based on colony morphological observation, physiological and biochemical tests, as well as living/dead cells staining analysis and 16S rRNA gene sequencing. Read More

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http://dx.doi.org/10.1016/j.micpath.2020.104371DOI Listing

Cyclodextrin nanosponge as a Temoporfin nanocarrier: balancing between accumulation and penetration in 3D tumor spheroids.

Eur J Pharm Biopharm 2020 Jul 4. Epub 2020 Jul 4.

Université de Lorraine, CNRS, CRAN, F-54500 Nancy, France; Institut de Cancérologie de Lorraine, 6 avenue de Bourgogne, 54519 Vandoeuvre-lès-Nancy, France.

As the intertissue delivery of hydrophobic temoporfin (mTHPC) remains inefficient, we propose the use of cyclodextrin-based nanosponges as a smart, advanced system for improved mTHPC delivery. Recently, we demonstrated that cyclodextrins (CDs) allow mTHPC to penetrate into tumor spheroids via a nanoshuttle mechanism. However, the CD complexes were very sensitive to the dilution, thus limiting their translation invivo. Read More

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http://dx.doi.org/10.1016/j.ejpb.2020.06.022DOI Listing

Effects of dietary n-3 highly unsaturated fatty acids levels on growth, lipid metabolism and innate immunity in juvenile golden pompano (Trachinotus ovatus).

Fish Shellfish Immunol 2020 Jul 4. Epub 2020 Jul 4.

College of Marine Sciences of South China Agricultural University & Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou, 510642, China. Electronic address:

To investigate the effects of dietary n-3 highly unsaturated fatty acids (HUFA) levels on growth, lipid metabolism and innate immunity in juvenile golden pompano Trachinotus ovatus, a marine carnivorous teleost, a total of 450 fish (average body weight: 14.84 g) were randomly distributed into 18 cages at sea, each dietary group with three cages and respectively fed six diets (D1-D6) with 2.30% (D1), 0. Read More

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http://dx.doi.org/10.1016/j.fsi.2020.06.060DOI Listing

Piscidin 4: Genetic expression and comparative immunolocalization in Nile tilapia (Oreochromis niloticus) following challenge using different local bacterial strains.

Dev Comp Immunol 2020 Jul 4:103777. Epub 2020 Jul 4.

Cell Biology Department, Genetic Engineering and Biotechnology Division Research, National Research Centre, 33 El Buhouth St., 12622, Dokki, Giza, Egypt.

The antimicrobial activity of tilapia piscidin 4 (TP4) was determined in vitro against four bacterial strains, Aeromonas hydrophilla, Pseudomonas fluorescens, Streptococcus iniae and Vibrio anguillarum. Nile tilapia were infected with low and high doses of the tested pathogens; after 3, 6, 24 hours and 7 days of the specific TP4 gene expression, tissue immunolocalization was also performed. Histopathological examination revealed septicaemia and necrosis of hemopoietic tissue for all of the tested bacteria. Read More

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http://dx.doi.org/10.1016/j.dci.2020.103777DOI Listing

TLR13, TLR22, TRAF6, and TAK1 in the soiny mullet (Liza haematocheila): Molecular characterization and expression profiling analysis.

Dev Comp Immunol 2020 Jul 4:103774. Epub 2020 Jul 4.

Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai, 201306, China. Electronic address:

Toll-like receptors (TLRs) and their associated signaling pathways play pivotal roles in the immune response to invading pathogens. Here, TLR13, TLR22, tumor necrosis factor receptor-associated factor 6 (TRAF6), and transforming growth factor-β-activated kinase1 (TAK1) were characterized in the soiny mullet (Liza haematocheila), representative mugilid species that is widely cultured in Asia. The four mullet genes, which shared characteristic features with their counterparts in other teleosts, were ubiquitously expressed in all of the examined tissues, albeit with different expression patterns. Read More

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http://dx.doi.org/10.1016/j.dci.2020.103774DOI Listing

NLRC3-like 1 inhibits NOD1-RIPK2 pathway via targeting RIPK2.

Dev Comp Immunol 2020 Jul 4:103769. Epub 2020 Jul 4.

State Key Laboratory of Freshwater Ecology and Biotechnology, Key Laboratory of Aquaculture Disease Control, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, Hubei Province, 430072, China; Innovation Academy for Seed Design, Chinese Academy of Sciences, Beijing, China; University of Chinese Academy of Sciences, Beijing, China. Electronic address:

Both NLRC3 and NOD1 belong to regulatory NLR subfamily based on their best-characterized function. In mammals, NLRC3 was reported to function by attenuating signaling cascades initiated by other families of PRRs. In teleosts, multiple NLRC3-like genes were identified through transcriptome sequencing. Read More

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http://dx.doi.org/10.1016/j.dci.2020.103769DOI Listing

Research progress on viral accommodation 2009 to 2019.

Authors:
T W Flegel

Dev Comp Immunol 2020 Jul 4:103771. Epub 2020 Jul 4.

Center of Excellence for Shrimp Molecular Biology and Biotechnology (Centex Shrimp), Faculty of Science, Mahidol University, Rama VI Road, Phayathai, Bangkok 10300, Thailand; National Center for Genetic Engineering and Biotechnology (BIOTEC), National for Science and Technology Development Agency (NSTDA), Yothi Office, Rama VI Rd, Phayathai, Bangkok, 10400, Thailand.

The viral accommodation hypothesis for crustaceans and insects was first proposed in 1998/2001, stimulated by observations that shrimp and insects or insect cell lines can coexist with both DNA or RNA viruses without showing any signs of disease (i.e., they tolerate, single to multiple, persistent infections, sometimes for a lifetime). Read More

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http://dx.doi.org/10.1016/j.dci.2020.103771DOI Listing

Physalin A attenuates inflammation through down-regulating c-Jun NH2 kinase phosphorylation/Activator Protein 1 activation and up-regulating the antioxidant activity.

Toxicol Appl Pharmacol 2020 Jul 4:115115. Epub 2020 Jul 4.

Department of Pharmacology, China Medical University, Taichung 40402, Taiwan, ROC; Chinese Medicine Research Center, China Medical University, Taichung 40402, Taiwan, ROC; Drug Development Center, China Medical University, Taichung 40402, Taiwan, ROC. Electronic address:

Physalin A (PA), a withanolide, was isolated from Physalis angulata L. In this study, it is shown that PA can inhibit the production of inflammatory cytokines such as PGE, NO, IL-1β, IL-6, and TNF-α in LPS-induced RAW 264.7 cells. Read More

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http://dx.doi.org/10.1016/j.taap.2020.115115DOI Listing

Immunological and anticancer activities of seleno-ovalbumin (Se-OVA) on H22-bearing mice.

Int J Biol Macromol 2020 Jul 4. Epub 2020 Jul 4.

State Key Laboratory of Food Nutrition and Safety, Key Laboratory of Food Nutrition and Safety, Ministry of Education of China, College of Food Science and Engineering, Tianjin University of Science and Technology, Tianjin 300457, PR China.

Ovalbumin is the main protein component of egg white. Selenium is one of the essential trace elements. In our research, ovalbumin was modified into seleno-ovalbumin. Read More

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http://dx.doi.org/10.1016/j.ijbiomac.2020.07.006DOI Listing

Mechanisms of Cav3-associated arrhythmia: Protein or microdomain dysfunction?

Int J Cardiol 2020 Jul 4. Epub 2020 Jul 4.

Department of Pharmacology, University of California Davis, 451 Health Sciences Drive, Tupper Hall rm 2427, Davis, CA 95616, United States of America. Electronic address:

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http://dx.doi.org/10.1016/j.ijcard.2020.06.051DOI Listing

A radial force-independent bioprosthesis for transcatheter tricuspid valve implantation in a preclinical model.

Int J Cardiol 2020 Jul 4. Epub 2020 Jul 4.

Department of Cardiovascular Surgery, Changhai Hospital, The Second Military Medical University, Shanghai, China. Electronic address:

Background: Interventional treatments of tricuspid regurgitation have been emerging as minimally invasive alternatives. Among those reported transcatheter tricuspid devices, the radial force between the device and native tricuspid annulus is the common principle to be employed for device immobilization. However, this immobilization mechanism may potentially lead to adverse consequences. Read More

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http://dx.doi.org/10.1016/j.ijcard.2020.06.070DOI Listing

Heterologous expression of lytic polysaccharide monooxygenases (LPMOs). A mini-review.

Biotechnol Adv 2020 Jul 4:107583. Epub 2020 Jul 4.

Faculty of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences, Ås 1430, Norway. Electronic address:

Lytic polysaccharide monooxygenases (LPMOs) are relatively new enzymes that have been discovered 10 years ago. LPMOs comprise a diverse group of enzymes which play a pivotal role in the depolymerization of sugar-based biopolymers including cellulose, hemicellulose, chitin, and starch. Their mechanism of action relies on the correct coordination of a copper ion in the active site, which is partly composed of the N-terminal histidine. Read More

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http://dx.doi.org/10.1016/j.biotechadv.2020.107583DOI Listing

In vivo gene delivery mediated by non-viral vectors for cancer therapy.

J Control Release 2020 Jul 4. Epub 2020 Jul 4.

Sabanci University Nanotechnology Research and Application Center (SUNUM), Tuzla, Istanbul 34956, Turkey; Center of Excellence for Functional Surfaces and Interfaces (EFSUN), Faculty of Engineering and Natural Sciences, Sabanci University, Tuzla, Istanbul 34956, Turkey. Electronic address:

Gene therapy by expression constructs or down-regulation of certain genes has shown great potential for the treatment of various diseases. The wide clinical application of nucleic acid materials dependents on the development of biocompatible gene carriers. There are enormous various compounds widely investigated to be used as non-viral gene carriers including lipids, polymers, carbon materials, and inorganic structures. Read More

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http://dx.doi.org/10.1016/j.jconrel.2020.06.038DOI Listing

Multimodal Theranostics Augmented by Transmembrane Polymer-Sealed Nano-enzymatic Porous MoS Nanoflowers.

Int J Pharm 2020 Jul 4:119606. Epub 2020 Jul 4.

Minhang Hospital and School of Pharmacy, Key Laboratory of Smart Drug Delivery, Ministry of Education, Fudan University, Shanghai 201203, China. Electronic address:

Developing an all-in-one multimodal theranostic platform that can synergistically integrate sensitive photoacoustic (PA) imaging, enhanced photothermal therapy (PTT) and photodynamic therapy (PDT) as well as the nano-enzyme activated chemodynamic therapy (CDT) presents a great challenge for the current nanomedicine design. Herein, a simple hydrothermal method was used to prepare porous molybdenum disulfide (MoS) nanoflowers. These nanoflowers were assembled by three dimensional (3D)-stacked MoS nanosheets with plentiful pores and large surfaces, which thus exhibited enhanced photothermal conversion via light trapping and peroxidase (POD)-like activity via active defects exposure. Read More

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http://dx.doi.org/10.1016/j.ijpharm.2020.119606DOI Listing

Engineering an anti-granulocyte colony stimulating factor receptor nanobody for improved affinity.

Life Sci 2020 Jul 4:118052. Epub 2020 Jul 4.

Biosensor Research Center, Endocrinology and Metabolism Molecular-Cellular Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran. Electronic address:

Aims: Granulocyte colony-stimulating factor (G-CSF) is a cytokine that induces proliferation and differentiation of hematopoietic precursor cells and activation of mature neutrophils. G-CSF is overexpressed in several malignant tumors and blocking its binding to the receptor can lead to significant decrease in tumor growth, vascularization and metastasis. Furthermore, targeting G-CSF receptor has shown therapeutic benefit in other diseases such as rheumatoid arthritis, progressive neurodegenerative disorder and uveitis. Read More

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http://dx.doi.org/10.1016/j.lfs.2020.118052DOI Listing

Inhibition of miR-34a reduces cellular senescence in human adipose tissue-derived mesenchymal stem cells through the activation of SIRT1.

Life Sci 2020 Jul 4:118055. Epub 2020 Jul 4.

Department of Medical Biotechnology, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran; Urogenital Stem Cell Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran; Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Electronic address:

Aims: Human adipose derived mesenchymal stem cells (hAD-MSCs) as the most promising target for cell therapy and regenerative medicine, face senescence as a major drawback resulting in their limited proliferation and differentiation potentials. To evaluate the efficacy of miR-34a silencing as an anti-senescence strategy in hAD-MSCs, in this study common hallmarks of senescence were assessed after transient inhibition of miR-34a in hAD-MSCs.

Materials And Methods: The expression levels of miR-34a in hAD-MSCs at different passages were evaluated by real-time quantitative PCR. Read More

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http://dx.doi.org/10.1016/j.lfs.2020.118055DOI Listing

Seven new species of from conifers in Norway, Poland, and Russia.

Mycologia 2020 Jul 7:1-23. Epub 2020 Jul 7.

Department of Biochemistry, Genetics and Microbiology, Forestry and Agricultural Biotechnology Institute, University of Pretoria , Pretoria 0002, South Africa.

During surveys of insect-associated mycobiomes in Norway, Poland, and Russia, isolates with affinity to (Ophiostomatales, Ascomycota) were recovered. In this study, eight known species as well as the newly collected isolates were compared based on morphology and DNA sequence data for four gene regions. The results revealed seven new species, described here as , and . Read More

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http://dx.doi.org/10.1080/00275514.2020.1778375DOI Listing

A nuclear protein NsiA from Epichloë festucae interacts with a MAP kinase MpkB and regulates the expression of genes required for symbiotic infection and hyphal cell fusion.

Mol Microbiol 2020 Jul 7. Epub 2020 Jul 7.

Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa, Nagoya, 464-8601, Japan.

The endophytic fungus Epichloë festucae systemically colonizes the intercellular spaces of cool-season grasses to establish a mutualistic symbiosis. Hyphal growth of the endophyte within the host plant is tightly regulated and synchronized with the growth of the host plant. A genetic screen to identify symbiotic genes identified mutant FR405 that had an antagonistic interaction with the host plant. Read More

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http://dx.doi.org/10.1111/mmi.14568DOI Listing

Molecular phylogeny and distribution of dengue virus serotypes circulating in Nepal in 2017.

PLoS One 2020 7;15(7):e0234929. Epub 2020 Jul 7.

Central Department of Biotechnology, Tribhuvan University, Kirtipur, Kathmandu, Nepal.

Dengue virus (DENV) infection is endemic in Nepal. Although infection rates are reported annually, little information is available about the circulating viral serotypes and genotypes. Here, we report the results of a multicentre cross-sectional study of DENV serotypes and genotypes sampled from individuals with suspected DENV infection in Nepal in 2017. Read More

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0234929PLOS

Obesity is associated with senescence of mesenchymal stromal cells derived from bone marrow, subcutaneous and visceral fat of young mice.

Aging (Albany NY) 2020 Jul 7;12. Epub 2020 Jul 7.

Department of Experimental Medicine, Biotechnology and Molecular Biology Section, Second University of Naples, Naples, Italy.

White adipose tissue (WAT) is distributed in several depots with distinct metabolic and inflammatory functions. In our body there are subcutaneous (sWAT), visceral (vWAT) and bone marrow (bWAT) fat depots. Obesity affects the size, function and inflammatory state of WATs. Read More

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http://dx.doi.org/10.18632/aging.103606DOI Listing

Application of Graphene as a Nanoindenter Interacting with Phospholipid Membrane - Computer Simulation Study.

J Phys Chem B 2020 Jul 7. Epub 2020 Jul 7.

Synthesis of graphene in 2004 stimulated wide interest in potential application of 2D materials in catalysis, optoelectronics, biotechnology and construction of sensing devices. In the presented study interactions between graphene sheets and phospholipid bilayers are examined using steered molecular dynamics simulations. Graphene sheets of different sizes were inserted into bilayer and subsequently withdrawn from it at two different rates (1 m/s and 2 m/s). Read More

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http://dx.doi.org/10.1021/acs.jpcb.0c02319DOI Listing

Overcoming vincristine resistance in cancer: computational design and discovery of piperine inspired P-glycoprotein inhibitors.

Chem Biol Drug Des 2020 Jul 7. Epub 2020 Jul 7.

Centre for Bioinformatics, School of Life Sciences, Pondicherry University, Kalapet, Puducherry- 605014, India.

P-glycoprotein (P-gp)/MDR-1 plays a major role in the development of multidrug resistance (MDR) by pumping the chemotherapeutic drugs out of the cancer cells and reducing their efficacy. A number of P-gp inhibitors were reported to reverse the MDR when co-administered with chemotherapeutic drugs. Unfortunately, none has approved for clinical use due to toxicity issues. Read More

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http://dx.doi.org/10.1111/cbdd.13758DOI Listing

Molecular adaptation and convergent evolution of frugivory in Old World and neotropical fruit bats.

Mol Ecol 2020 Jul 7. Epub 2020 Jul 7.

Department of Ecology, Tibetan Centre for Ecology and Conservation at WHU-TU, Hubei Key Laboratory of Cell Homeostasis, College of Life Sciences, Wuhan University, Wuhan, 430072, China.

Although cases of independent adaptation to the same dietary niche have been documented in mammalian ecology, the molecular correlates of such shifts are seldom known. Here we used genome-wide analyses of molecular evolution to examine two lineages of bats that, from an insectivorous ancestor, have both independently evolved obligate frugivory: the Old World family Pteropodidae and the neotropical subfamily Stenodermatinae. New genome assemblies from two neotropical fruit bats (Artibeus jamaicensis and Sturnira hondurensis) provide a framework for comparisons with Old World fruit bats. Read More

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http://dx.doi.org/10.1111/mec.15542DOI Listing

Ecklonia radiata extract containing eckol protects neuronal cells against Aβ evoked toxicity and reduces aggregate density.

Food Funct 2020 Jul 7. Epub 2020 Jul 7.

Discipline of Pharmacology, Adelaide Medical School, Faculty of Health Sciences, The University of Adelaide, Adelaide, South Australia, Australia.

Brown seaweed (Phaeophyceae) polyphenolics such as phlorotannins are ascribed various biological activities, including neuroprotection. Of these seaweeds, Ecklonia radiata (E. radiata) is found abundantly along South Australian coastal regions; however it has not been explored for various biological activities relative to any component phlorotannins previously ascribed neuroprotective capacity. Read More

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http://dx.doi.org/10.1039/d0fo01438aDOI Listing

The assessment of intracellular magnesium: different strategies to answer different questions.

Magnes Res 2020 Feb;33(1):1-11

Department of Pharmacy, Biotechnology, University of Bologna, Bologna, Italy, National Institute of Biostructures, Biosystems (NIBB), Rome, Italy.

The role of magnesium in cell metabolism is complex and still not completely clarified. Although magnesium has been shown to modulate many phenomena in cells, its intracellular distribution and subcellular compartmentalization have not yet elucidated in detail, mainly as a consequence of the inadequacy of analytical techniques. The method usually employed to quantify total magnesium in cells or tissue are F-AAS or more sensitive techniques as graphite furnace AAS and inductively coupled plasma mass spectroscopy (MS). Read More

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http://dx.doi.org/10.1684/mrh.2020.0464DOI Listing
February 2020

A mechanistic model and therapeutic interventions for COVID-19 involving a RAS-mediated bradykinin storm.

Elife 2020 Jul 7;9. Epub 2020 Jul 7.

Biosciences, Oak Ridge National Laboratory, Oak Ridge, United States.

Neither the disease mechanism nor treatments for COVID-19 are currently known. Here we present a novel molecular mechanism for COVID-19 that provides therapeutic intervention points that can be addressed with existing FDA-approved pharmaceuticals. The entry point for the virus is ACE2, which is a component of the counteracting hypotensive axis of RAS, that produces the nonapeptide angiotensin from angiotensin I. Read More

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http://dx.doi.org/10.7554/eLife.59177DOI Listing

Dopamine role in learning and action inference.

Authors:
Rafal Bogacz

Elife 2020 Jul 7;9. Epub 2020 Jul 7.

Medical Research Council Brain Network Dynamics Unit, University of Oxford, Oxford, United Kingdom.

This paper describes a framework for modelling dopamine function in the mammalian brain. It proposes that both learning and action planning involve processes minimizing prediction errors encoded by dopaminergic neurons. In this framework, dopaminergic neurons projecting to different parts of the striatum encode errors in predictions made by the corresponding systems within the basal ganglia. Read More

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http://dx.doi.org/10.7554/eLife.53262DOI Listing

analysis of Calcium Dependent Protein Kinase 6 of Cr through molecular modeling, docking, and dynamics simulation study.

J Biomol Struct Dyn 2020 Jul 7:1-10. Epub 2020 Jul 7.

School of Biotechnology, KIIT Deemed to be University, Bhubaneswar, Odisha, India.

Calcium Dependent Protein Kinases are found in the Apicomplexan, algae, and plants; however, they are reported in vertebrates and are regarded as excellent drug targets for pharmaceutical interventions. Calcium Dependent Protein Kinases of are probably involved in the regulation of invasion and egress process during the infection of the host cells. The previous study reported that after the Calcium Dependent Protein Kinase 1 gene, Calcium Dependent Protein Kinase 6 of is expressed in all stages of the parasite (merozoites/schizonts as well as sexual stages) at a comparable level and makes it as a valid drug target. Read More

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http://dx.doi.org/10.1080/07391102.2020.1790036DOI Listing

Optimization of spore laccase production by isolated from wastewater and its potential in green biodecolorization of synthetic textile dyes.

Prep Biochem Biotechnol 2020 Jul 7:1-12. Epub 2020 Jul 7.

Microbiology Department, Central Laboratory for Environmental Quality-Monitoring (CLEQM), National Water Research Center, Cairo, Egypt.

The spore laccase enzyme production by was optimized. It was characterized and tested for its textile dye decolorization potential. LB medium was found to be the most promising growth medium with addition of glucose (1-2%), yeast extract (0. Read More

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http://dx.doi.org/10.1080/10826068.2020.1786698DOI Listing

The Proteomics of Resistance to Halo Blight in Common Bean.

Mol Plant Microbe Interact 2020 Jul 7. Epub 2020 Jul 7.

Embrapa Genetic Resources and Biotechnology, Brasilia, Brazil;

Halo blight disease of beans is caused by a Gram-negative bacterium, pv. (Pph). The disease is prevalent in South America and Africa and causes crop loss to indigent people who rely on beans as a primary source of daily nutrition. Read More

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http://dx.doi.org/10.1094/MPMI-05-20-0112-RDOI Listing

Transcriptome signature of two lactation stages in Ghezel sheep identifies using RNA-Sequencing.

Anim Biotechnol 2020 Jul 7:1-11. Epub 2020 Jul 7.

Genomics Research Platform, School of Life Sciences, La Trobe University, Melbourne, Victoria, Australia.

The expression of genes and their regulation during lactation in Ghezel sheep breed remains less understood. To explore the underlying molecular mechanism of the lactation process in the mammary gland, transcriptome profiles of Iranian fat-tailed Ghezel sheep breed milk at two stages, before (BF) and after peak (AF) stages of lactation were investigated. Functional impacts of differentially expressed genes (DEGs) between BF and AF stages were surveyed using Gene Ontology (GO) and Protein-Protein Interaction (PPI) network analysis. Read More

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http://dx.doi.org/10.1080/10495398.2020.1784185DOI Listing

Bromhexine and its inhibitory effect on lipase - kinetics and structural study.

Arch Physiol Biochem 2020 Jul 7:1-6. Epub 2020 Jul 7.

Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran.

Lipase hydrolyses the ester bonds in triglyceride. It is an important enzyme in medicine and industry. Some pathogen bacteria use this exoenzyme to disrupt the extracellular matrix of host organisms. Read More

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http://dx.doi.org/10.1080/13813455.2020.1788606DOI Listing

Dual lysine and N-terminal acetyltransferases reveal the complexity underpinning protein acetylation.

Mol Syst Biol 2020 Jul;16(7):e9464

Plant Physiology, Institute of Plant Biology and Biotechnology, University of Muenster, Muenster, Germany.

Protein acetylation is a highly frequent protein modification. However, comparatively little is known about its enzymatic machinery. N-α-acetylation (NTA) and ε-lysine acetylation (KA) are known to be catalyzed by distinct families of enzymes (NATs and KATs, respectively), although the possibility that the same GCN5-related N-acetyltransferase (GNAT) can perform both functions has been debated. Read More

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http://dx.doi.org/10.15252/msb.20209464DOI Listing

Neutrophil elastase triggers the development of autoimmune diabetes by exacerbating innate immune responses in pancreatic islets of non-obese diabetic mice.

Clin Sci (Lond) 2020 Jul;134(13):1679-1696

State Key Laboratory of Pharmaceutical Biotechnology, The University of Hong Kong, China.

Type 1 diabetes is an autoimmune disease resulted from self-destruction of insulin-producing pancreatic β cells. However, the pathological pathways that trigger the autoimmune destruction remain poorly understood. Clinical studies have demonstrated close associations of neutrophils and neutrophil elastase (NE) with β-cell autoimmunity in patients with Type 1 diabetes. Read More

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http://dx.doi.org/10.1042/CS20200021DOI Listing

Second-phase validation study of an alternative developmental toxicity test using mouse embryonic stem cell-derived embryoid bodies.

J Physiol Pharmacol 2020 Apr 2;71(2). Epub 2020 Jul 2.

Laboratory of Veterinary Biochemistry and Molecular Biology, College of Veterinary Medicine, Chungbuk National University, Cheongju, Chungbuk, Republic of Korea.

The embryoid body test (EBT) is a developmental toxicity test method that measures the size of embryoid bodies (EBs) and the viability of mouse embryonic stem cells (mESCs) and fibroblasts (3T3 cells). The previous pre-validation study confirmed the high accuracy (above 80%) of EBT using 26 coded test chemicals. This second-phase validation study assessed the inter-laboratory reproducibility (5 chemicals in common) and predictive capacity (10 chemicals in each laboratory) test using the coded test chemicals at three laboratories. Read More

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http://dx.doi.org/10.26402/jpp.2020.2.06DOI Listing

Comparison of phenolic compounds, antioxidant and cytotoxic activity of extracts prepared from Japanese quince (Chaenomeles japonica L.) leaves.

J Physiol Pharmacol 2020 Apr 2;71(2). Epub 2020 Jul 2.

Medical University of Lodz, Department of Biochemistry, Faculty of Medicine, Lodz, Poland.

Phenolic compounds are very important in the prevention and treatment of many civilization diseases, including colorectal cancer (CRC). In the present study we investigated and compared the phytochemical composition, antioxidant and cytotoxic activity of Japanese quince (Chaenomeles japonica L.) leaves crude phenolic extract (CPE) and purified phenolic-rich extracts (PRE). Read More

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http://dx.doi.org/10.26402/jpp.2020.2.05DOI Listing

Molecular basis for the recognition of steroidogenic acute regulatory protein by the 14-3-3 protein family.

FEBS J 2020 Jul 6. Epub 2020 Jul 6.

A.N, Bach Institute of Biochemistry, Federal Research Center of Biotechnology, Russian Academy of Sciences, 119071, Moscow, Russia.

Steroidogenesis in adrenals and gonads starts from cholesterol transport to mitochondria. This is mediated by the steroidogenic acute regulatory protein (STARD1), containing a mitochondrial import sequence followed by a cholesterol-binding START domain. Although mutations in this protein have been linked to lipoid congenital adrenal hyperplasia (LCAH), the mechanism of steroidogenesis regulation by STARD1 remains debatable. Read More

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http://dx.doi.org/10.1111/febs.15474DOI Listing

Genomic characteristics and drug screening among organoids derived from non-small cell lung cancer patients.

Thorac Cancer 2020 Jul 7. Epub 2020 Jul 7.

The Second School of Clinical Medicine, Southern Medical University, Guangzhou, China.

Background: Patient-derived organoid (PDO) models are highly valuable and have potentially widespread clinical applications. However, limited information is available regarding organoid models of non-small cell lung cancer (NSCLC). This study aimed to characterize the consistency between primary tumors in NSCLC and PDOs and to explore the applications of PDOs as preclinical models to understand and predict treatment response during lung cancer. Read More

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http://dx.doi.org/10.1111/1759-7714.13542DOI Listing

Micromorphological and cytological comparisons between Youngia japonica and Youngia longiflora using light and scanning electron microscopy.

Microsc Res Tech 2020 Jul 6:e23538. Epub 2020 Jul 6.

Department of Biological Science, College of Bioscience and Biotechnology, Chungnam National University, Daejeon, Republic of Korea.

The genus Youngia Cass. is taxonomically complex and widely distributed in East Asia. Despite the high diversity of species, only two species, Youngia japonica (L. Read More

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http://dx.doi.org/10.1002/jemt.23538DOI Listing

Revisiting potential druggable targets against SARS-CoV-2 and repurposing therapeutics under preclinical study and clinical trials: A comprehensive review.

Drug Dev Res 2020 Jul 6. Epub 2020 Jul 6.

ABEx Bio-Research Center, East Azampur, Dhaka, Bangladesh.

Coronavirus disease-19 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is one of the most contagious diseases in human history that has already affected millions of lives worldwide. To date, no vaccines or effective therapeutics have been discovered yet that may successfully treat COVID-19 patients or contain the transmission of the virus. Scientific communities across the globe responded rapidly and have been working relentlessly to develop drugs and vaccines, which may require considerable time. Read More

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http://dx.doi.org/10.1002/ddr.21709DOI Listing

Effects of the synthetic cannabinoid XLR-11 on the viability and migration rates of human brain microvascular endothelial cells in a clinically-relevant model.

Pharmacol Rep 2020 Jul 6. Epub 2020 Jul 6.

Department of Applied Biological Sciences, Jordan University of Science and Technology, Irbid, 22110, Jordan.

Background: Synthetic cannabinoids (SCs) are a group of newly-developed drugs that bind and activate endocannabinoid system receptors. Angiogenesis is a biological process in which new blood vessels are formed from preexistent blood vessels. It plays a vital role in tissue growth, wound healing, and embryogenesis. Read More

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http://dx.doi.org/10.1007/s43440-020-00123-0DOI Listing

Complete genome sequence of a novel potyvirus isolated from Polygonatum kingianum.

Arch Virol 2020 Jul 6. Epub 2020 Jul 6.

Key Laboratory for Agricultural Biodiversity for Pest Management of China, Ministry of Education, Yunnan Agricultural University, No. 95, Jinhei Road, Panlong District, Kunming, 650201, Yunnan, China.

The complete genome sequence of a putative novel potyvirus, tentatively named "Polygonatum kingianum virus 1" (PKgV1), infecting Polygonatum kingianum in China was determined (GenBank accession no. MK427056). PKgV1 has a genome organization that is typical of potyviruses, with a single large open reading frame (nt 123-9236) that encodes a 3037-aa polyprotein that is predicted to be cleaved into 10 mature proteins by virus-encoded proteases. Read More

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http://dx.doi.org/10.1007/s00705-020-04717-0DOI Listing

Efficient System Wide Metabolic Pathway Comparisons in Multiple Microbes Using Genome to KEGG Orthology (G2KO) Pipeline Tool.

Interdiscip Sci 2020 Jul 6. Epub 2020 Jul 6.

School of Basic Sciences, Indian Institute of Technology Mandi, Kamand, Himachal Pradesh, 175005, India.

Comparison of system-wide metabolic pathways among microbes provides valuable insights of organisms' metabolic capabilities that can further assist in rationally screening organisms in silico for various applications. In this work, we present a much needed, efficient and user-friendly Genome to KEGG Orthology (G2KO) pipeline tool that facilitates efficient comparison of system wide metabolic networks of multiple organisms simultaneously. The optimized strategy primarily involves automatic retrieval of the KEGG Orthology (KO) identifiers of user defined organisms from the KEGG database followed by overlaying and visualization of the metabolic genes using the KEGG Mapper reconstruct pathway tool. Read More

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http://dx.doi.org/10.1007/s12539-020-00375-7DOI Listing

Functional Analysis of Plant FYVE Domain Proteins in Endosomal Trafficking.

Methods Mol Biol 2020 ;2177:83-94

Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, School of Life Sciences, South China Normal University, Guangzhou, China.

The FYVE domain is a double zinc finger-like domain that predominantly binds phosphatidylinositol 3-phosphate. The FYVE domain is usually found in proteins primarily involved in regulating various aspects of endomembrane homeostasis, including endosome tethering, endocytic recycling, membrane protein sorting, and autophagosome maturation. Whereas FYVE domain proteins have been extensively studied in mammals and yeast, only a few FYVE domain proteins have been identified and characterized in plants. Read More

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http://dx.doi.org/10.1007/978-1-0716-0767-1_8DOI Listing
January 2020

Correction to: Camelids: new players in the international animal production context.

Trop Anim Health Prod 2020 Jul 6. Epub 2020 Jul 6.

Department of Animal Science, AU-Foulum, Aarhus University, Blichers Allé 20, Postboks 50, 8830, Tjele, Denmark.

In the originally published version of the article, Figure 4 image has an error. Corrected Fig. 4 is shown below. Read More

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http://dx.doi.org/10.1007/s11250-020-02341-3DOI Listing

QTL analysis of farinograph and mixograph related traits in spring wheat under heat stress conditions.

Mol Biol Rep 2020 Jul 6. Epub 2020 Jul 6.

College of Food Sciences and Agriculture, King Saud University, Riyadh, Saudi Arabia.

Farinograph and mixograph-related parameters are key elements in wheat end-products quality. Understanding the genetic control of these traits and the influence of environmental factors such as heat stress, and their interaction are critical for developing cultivars with improved for those traits. To identify QTL for six farinograph and three mixograph traits, two double haploid (DH) populations (Yecora Rojo × Ksu106 and Klasic × Ksu105) were used in experiments conducted at Riyadh and Al Qassim locations under heat stress. Read More

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http://dx.doi.org/10.1007/s11033-020-05638-6DOI Listing