375,491 results match your criteria Biotechnology & Biotechnological Equipment[Journal]


In silico analysis of different signal peptides for the secretory production of recombinant human keratinocyte growth factor in Escherichia coli.

Comput Biol Chem 2019 Mar 29;80:225-233. Epub 2019 Mar 29.

Department of Molecular Medicine, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran. Electronic address:

Background: The recombinant human truncated Keratinocyte growth factor (Palifermin) is the only FDA approved medicine for the treatment of oral mucositis. The Keratinocyte growth factor is a fairly unstable protein due to its high aggregation propensity and therefore its expression as a secretory protein may results in the production of a protein with more stability, higher solubility, better folding, enhanced biological activity, N-terminal authenticity and simplified downstream processing.

Objective: The aim of this study was in silico evaluation of 31 different secretory signal peptides to determine the best theoretical candidates for the secretory production of recombinant truncated human KGF in E. Read More

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http://dx.doi.org/10.1016/j.compbiolchem.2019.03.003DOI Listing

Discovery of 7H-pyrrolo[2,3-d]pyrimidine derivatives as selective covalent irreversible inhibitors of interleukin-2-inducible T-cell kinase (Itk).

Eur J Med Chem 2019 Mar 26;173:167-183. Epub 2019 Mar 26.

State Key Laboratory of Chemical Oncogenomics, Engineering Laboratory for Chiral Drug Synthesis, School of Chemical Biology and Biotechnology, Shenzhen Graduate School, Peking University, Shenzhen, 518055, China. Electronic address:

Interleukin-2-inducible T-cell kinase (Itk) plays an important role in multiple signal transduction pathways in T and mast cells, and is a potential drug target for treating inflammatory diseases, autoimmune diseases, and T cell leukemia/lymphoma. Herein, we describe the discovery of a series of covalent Itk inhibitors based on the 7H-pyrrolo[2,3-d]pyrimidine scaffold. Placing an appropriate substitution group at a hydration site of the ATP binding pocket of Itk and using a saturated heterocyclic ring as a linker to the reactive group were crucial for selectivity. Read More

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http://dx.doi.org/10.1016/j.ejmech.2019.03.055DOI Listing

Characterization of a Dibenzofuran-degrading strain of Pseudomonas aeruginosa, FA-HZ1.

Environ Pollut 2019 Apr 8;250:262-273. Epub 2019 Apr 8.

State Key Laboratory of Microbial Metabolism, School of Life Sciences & Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, People's Republic of China. Electronic address:

Dibenzofuran (DBF) derivatives have caused serious environmental problems, especially those produced by paper pulp bleaching and incineration processes. Prominent for its resilient mutagenicity and toxicity, DBF poses a major challenge to human health. In the present study, a new strain of Pseudomonas aeruginosa, FA-HZ1, with high DBF-degrading activity was isolated and identified. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S02697491193032
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http://dx.doi.org/10.1016/j.envpol.2019.04.026DOI Listing
April 2019
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Increasing DNA content for cost-effective oil production in Parachlorella kessleri.

Bioresour Technol 2019 Apr 11;285:121332. Epub 2019 Apr 11.

State Key Laboratory of Microbial Metabolism and School of Life Sciences & Biotechnology, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, China; Joint International Research Laboratory of Metabolic & Developmental Sciences, Shanghai Jiao Tong University, Shanghai 200240, China; Biomass Energy Research Center, Shanghai Jiao Tong University, Shanghai 200240, China. Electronic address:

The aim of this work was to study salt stress effects on DNA content and oil production processes integrating harvesting, lipid accumulation and oil extraction. Salt-induced enlargement of Parachlorella kessleri cells, with increasing content of DNA and neutral lipid were found. The 34. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S09608524193055
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http://dx.doi.org/10.1016/j.biortech.2019.121332DOI Listing
April 2019
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Optimization of operating conditions of miniaturize single chambered microbial fuel cell using NiWO/graphene oxide modified anode for performance improvement and microbial communities dynamics.

Bioresour Technol 2019 Apr 11;285:121337. Epub 2019 Apr 11.

School of Biochemical Engineering, Indian Institute of Technology (BHU), Varanasi, U.P. 221005, India. Electronic address:

NiWO and graphene oxide (NWG) modified anodic carbon cloth (CC) is used to improve the performance of single chambered microbial fuel cell (SMFC) by statistical optimization of operational parameters. The properties of synthesized NWG nanocomposite on the surface of modified anode are characterized by FTIR, XRD, EDX, TEM and SEM analysis. The optimum level of operational parameters maximize the power density (PD) 1458 mW/m of SMFC having NWG modified anode and observed 8. Read More

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http://dx.doi.org/10.1016/j.biortech.2019.121337DOI Listing

Immobilization of recombinant Escherichia coli whole cells harboring xylose reductase and glucose dehydrogenase for xylitol production from xylose mother liquor.

Bioresour Technol 2019 Apr 13;285:121344. Epub 2019 Apr 13.

Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou 310014, PR China; Engineering Research Center of Bioconversion and Biopurification of Ministry of Education, Zhejiang University of Technology, Hangzhou 310014, PR China.

In this study, recombinant E. coli BL21(DE3)/pCDFDuet-1-XR-GDH harboring xylose reductase (XR) and glucose dehydrogenase (GDH) were immobilized and applied for the production of xylitol from xylose mother liquor (XML). Various immobilization methods were screened and the cross-linking approach with diatomite and polyetherimide as the raw materials and glutaraldehyde as the cross-linking agent was the optimal one, and the recovery activity reached of 80. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S09608524193056
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http://dx.doi.org/10.1016/j.biortech.2019.121344DOI Listing
April 2019
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Chronic exposure to green light aggravates high-fat diet-induced obesity and metabolic disorders in male mice.

Ecotoxicol Environ Saf 2019 Apr 15;178:94-104. Epub 2019 Apr 15.

State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, 211198, PR China; School of Life Science and Technology, China Pharmaceutical University, Nanjing, 211198, PR China; State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing, 210023, PR China. Electronic address:

Light is involved in many critical physiological or biochemical processes of human beings, such as visual sensing and the production of vitamin D. Recent studies have showed that the lights of different wavelengths have a profound influence in life activities. For example, blue light promotes alertness, whereas green light (GL) induces sleep in mice. Read More

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http://dx.doi.org/10.1016/j.ecoenv.2019.04.013DOI Listing

Evaluation of the genotoxicity of neurotoxic insecticides using the micronucleus test in Tradescantia pallida.

Chemosphere 2019 Apr 12;227:371-380. Epub 2019 Apr 12.

Institute of Biotechnology, Federal University of Uberlândia, Campus Umuarama, 38900-402, Uberlândia, Minas Gerais, Brazil.

Neonicotinoids and phenylpyrazoles are classes of neurotoxic insecticides which are able to bind at different ligand sites of neural receptors, leading to the deregulation of insect neural activity and hence resulting in death. The misuse or indiscriminate use of these chemicals is directly associated with several toxicological effects in biota and at different trophic levels. Based on this premise, the aim of the present study was to evaluate and compare the genotoxic capacity of different concentrations of thiamethoxam (TMX), acetamiprid (ACP), imidacloprid (IMI) and fipronil (FP) through the Micronucleus Test in Tradescantia pallida (Trad-MCN). Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00456535193072
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http://dx.doi.org/10.1016/j.chemosphere.2019.04.073DOI Listing
April 2019
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Synthesis and biological evaluation of purine-pyrazole hybrids incorporating thiazole, thiazolidinone or rhodanine moiety as 15-LOX inhibitors endowed with anticancer and antioxidant potential.

Bioorg Chem 2019 Apr 1;87:821-837. Epub 2019 Apr 1.

Genetic Engineering and Biotechnology Research Institute (GEBRI), City for Scientific Research and Technology, Alexandria, Egypt.

Novel purine-pyrazole hybrids combining thiazoles, thiazolidinones and rhodanines, were designed and tested as 15-LOX inhibitors, potential anticancer and antioxidant agents. All tested compounds were found to be potent 15-LOX inhibitors with IC ranging from 1.76 to 6. Read More

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http://dx.doi.org/10.1016/j.bioorg.2019.03.076DOI Listing

Cochliobolus sp. acts as a biochemical modulator to alleviate salinity stress in okra plants.

Plant Physiol Biochem 2019 Apr 14;139:459-469. Epub 2019 Apr 14.

Department of Botany, Garden Campus, Abdul Wali Khan University, Mardan, Pakistan.

Salinity stress can severely affect the growth and production of the crop plants. Cheap and reliable actions are needed to enable the crop plants to grow normal under saline conditions. Modification at the molecular level to produce resistant cultivars is one of the promising, yet highly expensive techniques, whereas application of endophytes on the other hand are very cheap. Read More

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http://dx.doi.org/10.1016/j.plaphy.2019.04.019DOI Listing

Combination of the endogenous promoter-intron significantly improves salt and drought tolerance conferred by TdSHN1 transcription factor in transgenic tobacco.

Plant Physiol Biochem 2019 Apr 12;139:435-445. Epub 2019 Apr 12.

Laboratory of Plant Biotechnology and Improvement, University of Sfax, Center of Biotechnology of Sfax, Route Sidi Mansour, Km 6 B.P' 1177, 3018, Sfax, Tunisia. Electronic address:

Recent years have witnessed a renewed interest in introns as a tool to increase gene expression. We previously isolated TdSHN1 gene encoding a transcription factor in durum wheat. Here we show that TdSHN1 intron contains many CT-stretches and the motif CGATT known to be important for IME. Read More

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http://dx.doi.org/10.1016/j.plaphy.2019.04.009DOI Listing

Strontium-incorporated mineralized PLLA nanofibrous membranes for promoting bone defect repair.

Colloids Surf B Biointerfaces 2019 Apr 6;179:363-373. Epub 2019 Apr 6.

College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai, 201620, China. Electronic address:

Mineralized scaffolds, which are fabricated by electrodeposition, mimic the chemistry of natural bone and have attracted a great amount of attention due to their rapid and simple production. In this study, mineralized electrospun polylactic acid (PLLA) nanofibrous membranes containing different amounts of strontium (Sr) were fabricated by an electrodeposition method for potential use in bone regeneration applications. In vitro assays, including cell proliferation and osteogenic differentiation of rat bone marrow-derived mesenchymal stem cells (BMSCs) grown on these membranes and in vivo cranial bone defect repair assays, were carried out. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S09277765193023
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http://dx.doi.org/10.1016/j.colsurfb.2019.04.011DOI Listing
April 2019
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Molecular characterization of manganese superoxide dismutase (MnSOD) from sterlet Acipenser ruthenus and its responses to Aeromonas hydrophila challenge and hypoxia stress.

Comp Biochem Physiol A Mol Integr Physiol 2019 Apr 15. Epub 2019 Apr 15.

National Freshwater Fisheries Engineering Technology Research Center, Ministry of Science and Technology of China, Beijing Key Laboratory of Fishery Biotechnology (No.BZ0301), Beijing Fisheries Research Institute, Beijing 100068, China. Electronic address:

A novel gene encoding the mitochondrial manganese superoxide dismutase from sterlet Acipenser ruthenus (Ar-MnSOD) was cloned. The full-length cDNA of MnSOD was of 1040 bp with a 672 bp open reading frame encoding 224 amino acids and the deduced amino acid sequence was located in mitochondria. Sequence comparison analysis showed that Ar-MnSOD was highly similar to MnSODs of invertebrates and vertebrates, especially those of freshwater Cyprinidae fishes and mammals. Read More

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http://dx.doi.org/10.1016/j.cbpa.2019.04.010DOI Listing

Reconciling the controversial data on the effects of C fullerene at the organismal and molecular levels using as a model Drosophila melanogaster.

Toxicol Lett 2019 Apr 15. Epub 2019 Apr 15.

Taras Shevchenko National University of Kyiv, Volodymyrska St., 64, 01601, Kyiv, Ukraine.

Fullerenes have attracted attention since their discovery as structural units of complex carbon nanostructures capable of transporting drugs and macromolecules. As such artificial nanomaterials are applied in biology and medicine, they are routinely scrutinized for their effects on living organisms. The results of such studies range from direct destabilizing effects on DNA molecules to amelioration of the toxic effects of known genotoxic agents. Read More

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http://dx.doi.org/10.1016/j.toxlet.2019.03.006DOI Listing

Conjugal transfer of PMQR from uropathogenic E.coli under high ciprofloxacin selection pressure generates gyrA mutation.

Microb Pathog 2019 Apr 15. Epub 2019 Apr 15.

Department of Biochemistry and Medical Biotechnology, Calcutta School of Tropical Medicine, Kolkata, India. Electronic address:

Incidence of high fluoroquinolone resistance has been rising rapidly worldwide. Resistance against fluoroquinolones can be either chromosomal or plasmid mediated. Plasmid mediated quinolone resistant(PMQR) genes impart low level of resistance against fluoroquinolones but provides favorable background for selection of additional chromosomally encoded resistance mechanisms. Read More

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http://dx.doi.org/10.1016/j.micpath.2019.04.021DOI Listing

Comparison of MALDI-TOF MS Biotyper and 16S rDNA sequencing for identification of Pseudomonas species isolated from fish.

Microb Pathog 2019 Apr 15. Epub 2019 Apr 15.

Institute of Food and Environmental Hygiene, Faculty of Veterinary Medicine, Latvia University of Life Sciences and Technologies, K. Helmaņa iela 8, LV-3004, Jelgava, Latvia.

Effective and reliable methods of identification of Pseudomonas spp. are important for characterization of microorganisms. The aim of present study was to compare identification conducted with MALDI-TOF MS Biotyper and 16S rDNA sequencing of Pseudomonas species isolated from fish. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S08824010193042
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http://dx.doi.org/10.1016/j.micpath.2019.04.024DOI Listing
April 2019
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MPL nano-liposomal vaccine containing P5 HER2/neu-derived peptide pulsed PADRE as an effective vaccine in a mice TUBO model of breast cancer.

J Control Release 2019 Apr 15. Epub 2019 Apr 15.

Biotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran; Department of Pharmaceutical Nanotechnology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran. Electronic address:

Liposomal peptide-based vaccines can potentially suppress cancer cells proliferation in the host. To enhance the effectiveness of vaccination against cancer, additional strategies should also be employed. One strategy to promote peptide-based vaccine efficacy and induce powerful immune responses, is simultaneous activation of CD4 and CD8 T cells. Read More

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http://dx.doi.org/10.1016/j.jconrel.2019.04.019DOI Listing

Dysregulation of Akt-FOXO1 pathway leads to dysfunction of regulatory T cells in psoriasis patients.

J Invest Dermatol 2019 Apr 15. Epub 2019 Apr 15.

Department of Dermatology, Xijing Hospital, Fourth Military Medical University, Xi'an, China. Electronic address:

Psoriasis is a T lymphocyte-driven systemic inflammatory disease. Regulatory T (Treg) cells are essential for establishing and maintaining immune tolerance. In this study, we found that the psoriatic patients and healthy controls had comparable percentages of circulating CD4CD25FOXP3 Treg cells, but psoriatic Treg cells had reduced suppressive function. Read More

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http://dx.doi.org/10.1016/j.jid.2018.12.035DOI Listing

Improvement of soluble expression of GM-CSF in the cytoplasm of Escherichia coli using chemical and molecular chaperones.

Protein Expr Purif 2019 Apr 15. Epub 2019 Apr 15.

Department of Pharmaceutical Biotechnology, Isfahan Pharmaceutical Research Center, Faculty of Pharmacy, Isfahan University of Medical Sciences, Isfahan, Iran. Electronic address:

The most common approaches to improve soluble expression of heterologous proteins are applications of molecular chaperones such as DnaK, DnaJ, GrpE, GroEL and GroES. The aim of present study was to enhance soluble expression of granulocyte-macrophage colony-stimulating factor (GM-CSF) in Escherichia coli by different approaches including modification of cultivation and induction conditions, and thermally, genetically and chemically enhancement of expression of cellular chaperones. To genetically enhance amount of molecular chaperones, co-expression of pET28-GM-CSF and pKJE7 plasmids was performed. Read More

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http://dx.doi.org/10.1016/j.pep.2019.04.002DOI Listing

Glyphaea brevis - In vitro antioxidant and in silico biological activity of major constituents and molecular docking analyses.

Toxicol In Vitro 2019 Apr 15. Epub 2019 Apr 15.

Phytomedicine, Biochemical Pharmacology and Toxicology Laboratories, Department of Biochemistry, School of Sciences, PMB 704, The Federal University of Technology, Akure, Nigeria.

Previous studies have revealed that leaf extracts of Glyphaea brevis possess antioxidant activity but the bioactivity and mechanisms of action of its major constituents remain unknown. This study evaluated in vitro antioxidant and free radical scavenging activities of Glyphaea brevis twigs and leaves, and probable toxicity profile, pharmacological activities and mechanisms of action of major phytoconstituents in silico. Phytochemical screening detected saponins, tannins, steroids, anthraquinones, flavonoids, terpenoids and phenolics in the extracts. Read More

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http://dx.doi.org/10.1016/j.tiv.2019.04.013DOI Listing

Leishmaniasis in Istanbul; A new epidemiological data about refugee leishmaniasis.

Acta Trop 2019 Apr 15. Epub 2019 Apr 15.

Department of Medical Microbiology, Bakırköy Dr. Sadi Konuk Training and Research Hospital, University of Health Sciences, Istanbul, Turkey.

Leishmania is an intracellular parasite, which is transmitted by the bite of infected female Phlebotominae sand flies. Turkey is a crossroad between Europe and Asia that makes it important in terms of epidemiology. In the present study, we aimed to evaluate Leishmania infection among non-autochthonous patients admitted to Health Sciences University, Dr. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S0001706X193037
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http://dx.doi.org/10.1016/j.actatropica.2019.04.008DOI Listing
April 2019
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Genome sequencing and transcript analysis of Hemileia vastatrix reveal expression dynamics of candidate effectors dependent on host compatibility.

PLoS One 2019 18;14(4):e0215598. Epub 2019 Apr 18.

Departamento de Fitopatologia, Universidade Federal de Lavras, Lavras, Minas Gerais, Brazil.

Coffee leaf rust caused by the fungus Hemileia vastatrix is one of the most important leaf diseases of coffee plantations worldwide. Current knowledge of the H. vastatrix genome is limited and only a small fraction of the total fungal secretome has been identified. Read More

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0215598PLOS

βC1 protein encoded in geminivirus satellite concertedly targets MKK2 and MPK4 to counter host defense.

PLoS Pathog 2019 Apr 18;15(4):e1007728. Epub 2019 Apr 18.

State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou, China.

Plant viruses have evolved multiple strategies to overcome host defense to establish an infection. Here, we identified two components of a host mitogen-activated protein kinase (MAPK) cascade, MKK2 and MPK4, as bona fide targets of the βC1 protein encoded by the betasatellite of tomato yellow leaf curl China virus (TYLCCNV). βC1 interacts with the kinase domain of MKK2 and inhibits its activity. Read More

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http://dx.doi.org/10.1371/journal.ppat.1007728DOI Listing

Identification of the minimum region of flatfish myostatin propeptide (Pep45-65) for myostatin inhibition and its potential to enhance muscle growth and performance in animals.

PLoS One 2019 18;14(4):e0215298. Epub 2019 Apr 18.

Department of Marine Molecular Bioscience, Gangneung-Wonju National University, Gangneung-si, Ganwon-do, Korea.

Myostatin (MSTN) negatively regulates skeletal muscle growth, and its activity is inhibited by the binding of MSTN propeptide (MSTNpro), the N-terminal domain of proMSTN that is proteolytically cleaved from the proMSTN. Partial sequences from the N-terminal side of MSTNpro have shown to be sufficient to inhibit MSTN activity. In this study, to determine the minimum size of flatfish MSTNpro for MSTN inhibition, various truncated forms of flatfish MSTNpro with N-terminal maltose binding protein (MBP) fusion were expressed in E. Read More

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0215298PLOS

Two components of the rhpPC operon coordinately regulate the type III secretion system and bacterial fitness in Pseudomonas savastanoi pv. phaseolicola.

PLoS Pathog 2019 Apr 18;15(4):e1007673. Epub 2019 Apr 18.

Guangdong Key Lab of Biotechnology for Plant Development, School of Life Sciences, South China Normal University, Guangzhou, China.

Many plant bacterial pathogens including Pseudomonas species, utilize the type III secretion system (T3SS) to deliver effector proteins into plant cells. Genes encoding the T3SS and its effectors are repressed in nutrient-rich media but are rapidly induced after the bacteria enter a plant or are transferred into nutrient-deficient media. To understand how the T3SS genes are regulated, we screened for P. Read More

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http://dx.doi.org/10.1371/journal.ppat.1007673DOI Listing

Epidemiological trend of chikungunya outbreak in Pakistan: 2016-2018.

PLoS Negl Trop Dis 2019 Apr 18;13(4):e0007118. Epub 2019 Apr 18.

Department of Virology, National Institute of Health, Chak Shahzad, Islamabad, Pakistan.

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http://dx.doi.org/10.1371/journal.pntd.0007118DOI Listing

Azospirillum brasilense promotes increases in growth and nitrogen use efficiency of maize genotypes.

PLoS One 2019 18;14(4):e0215332. Epub 2019 Apr 18.

Department of Agronomy, Universidade Estadual de Londrina, Londrina, Paraná, Brazil.

The development of cultivars with an improved nitrogen use efficiency (NUE) together with the application of plant growth-promoting bacteria is considered one of the main strategies for reduction of fertilizers use. In this sense, this study: i) evaluated the effect of Azospirillum brasilense on the initial development of maize genotypes; ii) investigated the influence of A. brasilense inoculation on NUE under nitrogen deficit; and iii) sought for more NUE genotypes with higher responsiveness to A. Read More

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0215332PLOS

Cranial Trepanation: An Ancient Neurosurgical Therapy? Thoughts of a Follower of Positivist Medicine and Anthropology.

J Craniofac Surg 2019 Apr 12. Epub 2019 Apr 12.

Centre of Research in Osteoarchaeology and Paleopathology, Department of Biotechnology and Life Sciences, University of Insubria, Varese, Italy.

The authors' aim is to define a framework around the history of studies and analyses on cranial trepanation. In addition, based on the analytical approach of Abele de Blasio, the authors would like to reach an understanding of the various different interpretations of the origin and aetiology of the art of cranial trepanation, starting in the prehistoric era. In this brief study, historical discussions are intertwined with ethnoiatric and anthropometric techniques of the author, leading the reader into a fascinating discussion on the practice of trepanation in ancient populations. Read More

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http://dx.doi.org/10.1097/SCS.0000000000005545DOI Listing

Meeting Overview: Interferon Lambda-Disease Impact and Therapeutic Potential.

J Interferon Cytokine Res 2019 Apr 17. Epub 2019 Apr 17.

4 Laboratory of Translational Genomics, Division of Cancer Epidemiology and Genetics, National Cancer Institute, National Institutes of Health, Bethesda, Maryland.

A meeting entitled, "Interferon Lambda: Disease Impact and Translational Potential," was held on the campus of the National Institutes of Health in Bethesda, Maryland, on October 25-26, 2018. To our knowledge, this was the first meeting that focused exclusively on interferon lambda (IFN-λ). The meeting's purpose was to enhance interdisciplinary communication and promote new collaborations. Read More

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http://dx.doi.org/10.1089/jir.2019.0018DOI Listing

Biological, Pathological, and Molecular Characteristics of a New Potyvirus, Dendrobium Chlorotic Mosaic Virus, Infecting Orchid.

Plant Dis 2019 Apr 18:PDIS10181839RE. Epub 2019 Apr 18.

1 Program in Microbial Genomics, National Chung Hsing University and Academia Sinica, Taichung 40227, Taiwan.

is one of the popular orchids in Taiwan. This report describes a new potyvirus tentatively named Dendrobium chlorotic mosaic virus (DeCMV) causing chlorotic and mosaic symptoms in . . Read More

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http://dx.doi.org/10.1094/PDIS-10-18-1839-REDOI Listing

Link between Low-Fouling and Stealth: A Whole Blood Biomolecular Corona and Cellular Association Analysis on Nanoengineered Particles.

ACS Nano 2019 Apr 18. Epub 2019 Apr 18.

Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity , The University of Melbourne , Parkville , Victoria 3010 , Australia.

Upon exposure to human blood, nanoengineered particles interact with a multitude of plasma components, resulting in the formation of a biomolecular corona. This corona modulates downstream biological responses, including recognition by and association with human immune cells. Considerable research effort has been directed toward the design of materials that can demonstrate a low affinity for various proteins (low-fouling materials) and materials that can exhibit low association with human immune cells (stealth materials). Read More

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http://dx.doi.org/10.1021/acsnano.9b00552DOI Listing

Isolation and characterization of a novel scFv antibody fragments specific for Hsp70 as a tumor biomarker.

J Cell Biochem 2019 Apr 18. Epub 2019 Apr 18.

Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.

Many studies have shown that more than 50% of tumors express heat shock protein 70 kDa (Hsp70) at the plasma membrane surface while not seen in normal cells, therefore it is a promising therapeutic target in human cancers. Hence, we used phage display technology to produce a single-chain fragment variable (scFv) antibody against human Hsp70. For this, a target peptide from human Hsp70 was designed using bioinformatics studies and was chemically synthesized. Read More

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https://onlinelibrary.wiley.com/doi/abs/10.1002/jcb.28732
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http://dx.doi.org/10.1002/jcb.28732DOI Listing
April 2019
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A numbering-up strategy of hydrodynamic microfluidic filters for continuous-flow high-throughput cell sorting.

Lab Chip 2019 Apr 18. Epub 2019 Apr 18.

Department of Applied Chemistry and Biotechnology, Graduate School of Engineering, Chiba University, 1-33 Yayoi-cho, Inage-ku, Chiba 263-8522, Japan.

Even though a number of microfluidic systems for particle/cell sorting have been proposed, facile and versatile platforms that provide sufficient sorting throughput and good operability are still under development. Here we present a simple but effective numbering-up strategy to dramatically increase the throughput of a continuous-flow particle/cell sorting scheme based on hydrodynamic filtration (HDF). A microfluidic channel equipped with multiple branches has been employed as a unit structure for size-based filtration, which realizes precise sorting without necessitating sheath flows. Read More

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http://dx.doi.org/10.1039/c9lc00053dDOI Listing

[Mutation Spectrum of FANCJ Gene in Adult Acute Myeloid Leukemia].

Zhongguo Shi Yan Xue Ye Xue Za Zhi 2019 Apr;27(2):348-353

Department of Hematology, The First Affiliated Hospital of Soochow University, Suzhou 215006, Jiangsu Province, China,E-mail:

Objective: To detect and analyze the mutation status of FANCJ gene in adult AML patients, so as to provide the basis for studying the mechanism of FANCJ driven AML and guiding the preventim and treatment of deseese.

Methods: The cDNAs were extracted and transeripted from bone marrow cells and normal skin cells in 222 newly diagnosed AML patients. The primers were designed for FANCJ gene coding region, the mutations of FANCJ gene coding region in AML patients as well as the mutations of FANCJ gene in mucous membrane epethelia in patients were detected by PCR and sanger seguencing; the evolutionary conservation of FANCJ mutation in different organisms was analyzed by NCBI Blast online bioinformaties software. Read More

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http://dx.doi.org/10.19746/j.cnki.issn.1009-2137.2019.02.006DOI Listing

Voltage-dependent anion channel isoform 3 as a potential male contraceptive drug target.

Future Med Chem 2019 Apr 18. Epub 2019 Apr 18.

Laboratory of Bioenergetics, Institute of Molecular Biology & Biotechnology, Faculty of Biology, Adam Mickiewicz University, Poznań, Poland.

Voltage-dependent anion channel isoform 3 (VDAC3), a channel in the mitochondrial outer membrane, has been suggested to play a role in the regulation of ATP transport and Ca homeostasis. These processes are regarded as important for spermatozoa motility. Accordingly, in previous years, mutations in the VDAC3-encoding gene were detected in spermatozoa with low motility from infertile patients. Read More

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https://www.future-science.com/doi/10.4155/fmc-2018-0328
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http://dx.doi.org/10.4155/fmc-2018-0328DOI Listing
April 2019
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Small-molecule inhibitors of lysine methyltransferases SMYD2 and SMYD3: current trends.

Future Med Chem 2019 Apr 18. Epub 2019 Apr 18.

Department of Pharmacy & Biotechnology, Alma Mater Studiorum University of Bologna, via Belmeloro 6, 40126 Bologna, Italy.

Lysine methyltransferases SMYD2 and SMYD3 are involved in the epigenetic regulation of cell differentiation and functioning. Overexpression and deregulation of these enzymes have been correlated to the insurgence and progression of different tumors, making them promising molecular targets in cancer therapy even if their role in tumors is not yet fully understood. In this light, selective small-molecule inhibitors are required to fully understand and validate these enzymes, as this is a prerequisite for the development of successful targeted therapeutic strategies. Read More

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http://dx.doi.org/10.4155/fmc-2018-0380DOI Listing

Differentiating Human Induced Pluripotent Stem Cells (iPSCs) Into Lung Epithelial Cells.

Curr Protoc Stem Cell Biol 2019 Apr 18:e86. Epub 2019 Apr 18.

Eyestem Research, Centre for Cellular and Molecular Platforms (CCAMP), National Centre for Biological Sciences (NCBS) Campus, Bengaluru, India.

Human induced pluripotent stem cells (hiPSCs) not only offer great opportunities for the study of human development but also have tremendous potential for future clinical cell-based therapies. The protocol outlined here is used to differentiate hiPSCs into lung epithelial cell types through a process that faithfully recapitulates the stepwise events observed in vivo. From pluripotency, cells are differentiated to a definitive endoderm fate, followed by progression into anteriorized foregut endoderm that has the ability to give rise to both proximal and distal epithelial cells. Read More

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https://onlinelibrary.wiley.com/doi/abs/10.1002/cpsc.86
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http://dx.doi.org/10.1002/cpsc.86DOI Listing
April 2019
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Surface-Independent and Oriented Immobilization of Antibody via One-Step Polydopamine/Protein G Coating: Application to Influenza Virus Immunoassay.

Macromol Biosci 2019 Apr 18:e1800486. Epub 2019 Apr 18.

Bionanotechnology Research Center, KRIBB, Daejeon, 34141, Korea.

For the construction of high-performance biosensor, it is important to interface bioreceptors with the sensor surface densely and in the optimal orientation. Herein, a simple surface modification method that can optimally immobilize antibodies onto various kinds of surfaces is reported. For the surface modification, a mixture of polydopamine (PDA) and protein G was employed. Read More

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http://dx.doi.org/10.1002/mabi.201800486DOI Listing

Bacterial community structure and function distinguish gut sites in captive red-shanked doucs (Pygathrix nemaeus).

Am J Primatol 2019 Apr 18:e22977. Epub 2019 Apr 18.

Department of Computer Science and Engineering, University of Minnesota, Minneapolis, Minnesota.

The mammalian order primates contains wide species diversity. Members of the subfamily Colobinae are unique amongst extant primates in that their gastrointestinal systems more closely resemble those of ruminants than other members of the primate order. In the growing literature surrounding nonhuman primate microbiomes, analysis of microbial communities has been limited to the hindgut, since few studies have captured data on other gut sites, including the foregut of colobine primates. Read More

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http://dx.doi.org/10.1002/ajp.22977DOI Listing

IN VITRO AND IN VIVO INVESTIGATION OF CHLOROPHYLL BINDING SITES INVOLVED IN NON-PHOTOCHEMICAL QUENCHING IN CHLAMYDOMONAS REINHARDTII.

Plant Cell Environ 2019 Apr 18. Epub 2019 Apr 18.

Department of Biotechnology, University of Verona, Strada Le Grazie 15, 37134, Verona, Italy.

Non-photochemical quenching (NPQ) of the light energy absorbed is one of the main photoprotective mechanisms evolved by oxygenic photosynthetic organisms to avoid photodamage, at a cost of reduced photosynthetic efficiency. Tuning of NPQ has been reported as a promising biotechnological strategy to increase productivity in both higher plants and unicellular microalgae. Engineering of NPQ induction requires the comprehension of its molecular mechanism(s), strongly debated in the last three decades with several different models proposed. Read More

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http://dx.doi.org/10.1111/pce.13566DOI Listing

Genetic diversity and structural analysis of 4-diphosphocytidyl-2C-methyl-D-erythritol kinase (IspE) from .

J Vector Borne Dis 2018 Oct-Dec;55(4):271-281

Protein Biochemistry and Structural Biology Laboratory, ICMR-National Institute of Malaria Research, New Delhi, India.

Background & Objectives: Plasmodium parasite harbours unique methylerythritol phosphate (MEP) pathway which is obligatory for the biosynthesis of isoprenoids. In malaria parasites, the isoprenoids are indispensable during hepatic, erythrocytic and gametocytic stages. Owing to the criticality of MEP pathway and the potential of its enzymes to act as antimalarial drug target, this study comprehensively investigated the genetic diversity and structural composition of 4-diphosphocytidyl-2C-methyl-D-erythritol kinase (IspE), fourth enzyme of MEP pathway in Indian Plasmodium falciparum (PfIspE). Read More

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http://dx.doi.org/10.4103/0972-9062.256562DOI Listing
April 2019
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Reconsidering osteoconduction in the era of additive manufacturing.

Authors:
Franz E Weber

Tissue Eng Part B Rev 2019 Apr 18. Epub 2019 Apr 18.

Zentrum fur Zahnmedizin der Universitat Zurich, 30992, MKG/Oral Biotechnology&Bioengineering , Plattenstrasse 11 , Zurich, Switzerland , 8032 ;

Bone regeneration procedures in clinics and bone tissue-engineering stand on three pillars: osteoconduction, osteoinduction, and stem cells. In the last two decades, the focus in this field has been on osteoinduction, realized by the use of bone morphogenetic proteins (BMPs) and the application of mesenchymal stem cells to treat bone defects. Osteoconduction, however, was reduced to a surface phenomenon, since the supposedly ideal pore size of osteoconductive scaffolds was identified in the 1990s, and with 0. Read More

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https://www.liebertpub.com/doi/10.1089/ten.TEB.2019.0047
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http://dx.doi.org/10.1089/ten.TEB.2019.0047DOI Listing
April 2019
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Patient-reported outcomes in hip resurfacing versus conventional total hip arthroplasty: a register-based matched cohort study of 726 patients.

Acta Orthop 2019 Apr 18:1-10. Epub 2019 Apr 18.

c Swedish Hip Arthroplasty Register , Gothenburg ;

Background and purpose - The theoretical mechanical advantages of metal-on-metal hip resurfacing (MoM-HR) compared with conventional total hip arthroplasty (THA) have been questioned. Studies including measures of patient-reported function, physical activity, or health-related quality of life have been sparse. We compared patient-reported outcomes in MoM-HR patients with a matched group of patients with conventional THA at 7 years post-surgery. Read More

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http://dx.doi.org/10.1080/17453674.2019.1604343DOI Listing

Total RNA quality in boar spermatozoa with different freezability.

Pol J Vet Sci 2019 Mar;22(1):181-185

Department of Animal Biochemistry and Biotechnology, Faculty of Animal Bioengineering, University of Warmia and Mazury in Olsztyn, 10-719 Olsztyn, Poland.

In this study the quality of total RNA, isolated from fresh spermatozoa, was compared between boars with good and poor semen freezability (GSF and PSF, respectively). Semen from 3 boars with GSF exhibited significantly higher total motility, mitochondrial function, plasma membrane integrity and reduced lipid peroxidation compared with 3 boars with PSF after cryo- preservation. There were variations in the quality of RNA isolated from spermatozoa of boars with GSF and PSF. Read More

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http://dx.doi.org/10.24425/pjvs.2019.127085DOI Listing

Quality parameters and fertility of ram semen cryopreserved in egg yolk and soybean lecithin supplemented extenders.

Pol J Vet Sci 2019 Mar;22(1):177-179

Department of Animal Reproduction Biotechnology, National Research Institute of Animal Production , Kraków , 32-083 Balice/Kraków, Poland.

The aim of the study was to investigate the effect of soybean lecithin as a substitute for egg yolk in milk and tris based extenders in ram semen cryopreservation. Twenty ejaculates were col- lected from four healthy, mature Wrzosówka rams (2-3 years of age). Each ejaculate was divided into four equal aliquots and diluted with four different extenders: 1) milk extender containing 5% egg yolk, 2) milk extender containing 1. Read More

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http://dx.doi.org/10.24425/pjvs.2019.127084DOI Listing

Production of ZFN-mediated GGTA1 knock-out pigs by microinjection of gene constructs into pronuclei of zygotes.

Pol J Vet Sci 2019 Mar;22(1):91-100

Department of Biochemistry and Biotechnology, Poznan University of Life Sciences, Dojazd 11, 60-632, Poznań, Poland.

Animals as a source of organs and tissues for xenotransplantation could become a backup solution for the growing shortage of human donors. The presence of human xenoreactive anti- bodies directed against Galα1,3Gal antigens on the cell surface of a pig donor triggers the activa- tion of the complement leading to a hyperacute reaction. The development of genetic engineer- ing techniques has enabled the modification of genomes by knocking in and/or knocking out genes. Read More

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http://dx.doi.org/10.24425/pjvs.2018.125611DOI Listing

The first detection of the sequence of bacteria from the Simkaniaceae family in surface waters in Poland.

Pol J Vet Sci 2019 Mar;22(1):61-65

Department of Microbiology, Faculty of Biology, University of Szczecin, Poland.

One of the most common reasons for horse lameness is subchondral bone cysts (SBCs), which are especially evident in young horse athletes. It is believed that SBC development is strongly associated with an individual's bone growth and/or bone microstructure impairment. Current methods of SBC treatment include pharmacological treatment or surgical procedures which may allow the bone within the cyst to rebuild and be restored to properly developed bone tissue. Read More

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http://dx.doi.org/10.24425/pjvs.2018.125608DOI Listing

Role of cytokines in combinatorial immunotherapeutics of non-small cell lung cancer through systems perspective.

Cancer Med 2019 Apr 17. Epub 2019 Apr 17.

National Centre for Cell Science, SP Pune University Campus, Pune, India.

Lung cancer is the leading cause of deaths related to cancer and accounts for more than a million deaths per year. Various new strategies have been developed and adapted for treatment; still the survival for 5 years is just 16% in patients with non-small cell lung cancer (NSCLC). Most of these strategies to combat NSCLC whether it is a drug molecule or immunotherapy/vaccine candidate require a big cost and time. Read More

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http://dx.doi.org/10.1002/cam4.2112DOI Listing

Top-down Mass Spectrometry of Intact Phosphorylated β-casein: Correlation between The Precursor Charge State and Internal Fragments.

J Mass Spectrom 2019 Apr 17. Epub 2019 Apr 17.

Mass Spectrometry and Proteomics Facility, The Ohio State University, Columbus, OH, 43210, USA.

Phosphorylated proteins play essential roles in many cellular processes, and identification and characterization of the relevant phosphoproteins can help to understand underlying mechanisms. Herein, we report a collision-induced dissociation top-down approach for characterizing phosphoproteins on a quadrupole time-of-flight mass spectrometer. β-casein, a protein with two major isoforms and five phosphorylatable serine residues, was used as a model. Read More

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http://dx.doi.org/10.1002/jms.4364DOI Listing

Potential of stem cell-derived exosomes to regenerate β islets through Pdx-1 dependent mechanism in a rat model of type 1 diabetes.

J Cell Physiol 2019 Apr 17. Epub 2019 Apr 17.

Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.

Type 1 diabetes, has been recognized as an autoimmune disease. Like other immunological conditions, regulation of immune response is a key strategy to control the autoimmunity in diabetic patients. Mesenchymal stem cells have been shown to have a distinct potential in modulating the immune reactions. Read More

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http://dx.doi.org/10.1002/jcp.28631DOI Listing