7,487 results match your criteria Biotechnologie Agronomie Societe Et Environnement[Journal]


First comprehensive view on a magnetic separation based protein purification processes: From process development to cleaning validation of a GMP-ready magnetic separator.

Eng Life Sci 2019 Aug 11;19(8):591-601. Epub 2019 Jul 11.

Institute of Functional Interfaces Karlsruhe Institute of Technology Eggenstein-Leopoldshafen Germany.

Magnetic separation processes are known as integrated bioanalytical protein purification method since decades and are well described. However, use of magnetic separation processes in a regulated industrial production environment has been prevented by the lack of suitable process equipment and prejudice against the productivity of the process and its qualification for cleaning-in-place operation. With the aim of overcoming this prejudice, a comprehensive process development approach is presented, based on a GMP-compliant magnetic separator, including an optimization of the batch adsorption process, implementation into a technical-scale, and the development and validation of cleaning routines for the device. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/elsc.201800183DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6999325PMC

Investigating flexible feeding effects on the biogas quality in full-scale anaerobic digestion by high resolution, photoacoustic-based NDIR sensing.

Eng Life Sci 2019 Oct 20;19(10):700-710. Epub 2019 Aug 20.

Escuela Politécnica Superior Universidad Autónoma de Madrid Madrid Spain.

In future energy systems based on renewable energies, biogas plants can make a significant contribution to stabilizing the electricity grids. However, this requires load-flexible and demand-oriented electricity production by means of flexible feed management. However, these flexible feeding strategies using greatly oscillating, temporally varying high mass loads may lead to critical process failures of the anaerobic digestion process. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/elsc.201900046DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6999453PMC
October 2019

Improving plant drought tolerance and growth under water limitation through combinatorial engineering of signaling networks.

Plant Biotechnol J 2020 Jul 5. Epub 2020 Jul 5.

Max-Planck-Institut für Molekulare Pflanzenphysiologie, Am Mühlenberg 1, D-14476, Potsdam-Golm, Germany.

Agriculture is by far the biggest water consumer on our planet, accounting for 70 percent of all freshwater withdrawals. Climate change and a growing world population increase pressure on agriculture to use water more efficiently ('more crop per drop'). Water-use efficiency (WUE) and drought tolerance of crops are complex traits that are determined by many physiological processes whose interplay is not well understood. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/pbi.13441DOI Listing

The relevance of enzyme specificity for coenzymes and the presence of 6-phosphogluconate dehydrogenase for polyhydroxyalkanoates production in the metabolism of Pseudomonas sp. LFM046.

Int J Biol Macromol 2020 Jul 2. Epub 2020 Jul 2.

University of São Paulo, Institute of Biomedical Sciences, Bioproducts Laboratory, Prof. Lineu Prestes Avenue, 1374 São Paulo, Brazil. Electronic address:

Reconstruction of genome-based metabolic model is a useful approach for the assessment of metabolic pathways, genes and proteins involved in the environmental fitness capabilities or pathogenic potential as well as for biotechnological processes development. Pseudomonas sp. LFM046 was selected as a good polyhydroxyalkanoates (PHA) producer from carbohydrates and plant oils. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ijbiomac.2020.06.226DOI Listing

Metabolic Signatures of Distinct Endothelial Phenotypes.

Trends Endocrinol Metab 2020 Jul 1. Epub 2020 Jul 1.

Laboratory of Angiogenesis and Vascular Metabolism, Department of Oncology, Katholieke Universiteit (KU) Leuven, Leuven, B 3000, Belgium; Laboratory of Angiogenesis and Vascular Metabolism, Vesalius Research Center, Vlaams Instituut voor Biotechnologie (VIB), Leuven, B 3000, Belgium. Electronic address:

Angiogenesis is crucial for the development of the blood vasculature during embryogenesis, but also contributes to cancer and other diseases. While therapeutic targeting of endothelial cells (ECs) through growth factor inhibition is limited by insufficient efficacy and resistance, a new paradigm for modulating angiogenesis by targeting EC metabolism has emerged. Findings from the past decade highlight how ECs adapt their metabolism to proliferate or migrate during vessel sprouting, or to maintain the vascular barrier and protect themselves against oxidative stress in the high-oxygen environment they are exposed to in healthy conditions. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.tem.2020.05.009DOI Listing

Natural Variation in Physicochemical Profiles and Bacterial Communities Associated with Aedes aegypti Breeding Sites and Larvae on Guadeloupe and French Guiana.

Microb Ecol 2020 Jul 3. Epub 2020 Jul 3.

Laboratory of Vector Control Research, Transmission Reservoir and Pathogens Diversity Unit, Institut Pasteur of Guadeloupe, Morne Jolivière, Guadeloupe, France.

Aedes aegypti develop in aquatic habitats in which mosquito larvae are exposed to physicochemical elements and microorganisms that may influence their life cycle and their ability to transmit arboviruses. Little is known about the natural bacterial communities associated with A. aegypti or their relation to the biotic and abiotic characteristics of their aquatic habitats. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00248-020-01544-3DOI Listing

Plant Cell Wall Proteomes: Bioinformatics and Cell Biology Tools to Assess the Bona Fide Cell Wall Localization of Proteins.

Methods Mol Biol 2020 ;2149:443-462

Laboratoire de Recherche en Sciences Végétales, UMR 5546 Université Paul Sabatier-Toulouse III/CNRS, Pôle de Biotechnologie Végétale, 24 chemin de Borderouge, Auzeville Tolosane, France.

The purification of plant cell walls is challenging because they constitute an open compartment which is not limited by a membrane like the cell organelles. Different strategies have been established to limit the contamination by proteins of other compartments in cell wall proteomics studies. Non-destructive methods rely on washing intact cells with various types of solutions without disrupting the plasma membrane in order to elute cell wall proteins. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/978-1-0716-0621-6_25DOI Listing
January 2020

Genome sequence analysis of the temperate bacteriophage TBP2 of the solvent producer Clostridium saccharoperbutylacetonicum N1-4 (HMT, ATCC 27021).

FEMS Microbiol Lett 2020 Jul 2. Epub 2020 Jul 2.

Institut für Mikrobiologie und Biotechnologie, Universität Ulm, Ulm, Germany.

The genus Clostridium consists of a diverse group of pathogenic and non-pathogenic bacteria. The non-pathogenic clostridia contain several solventogenic members of industrial importance, such as Clostridium acetobutylicum or C. beijerinckii. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1093/femsle/fnaa103DOI Listing

Dietary Patterns Are Associated with Risk of Prostate Cancer in a Population-Based Case-Control Study in Montreal, Canada.

Nutrients 2020 Jun 27;12(7). Epub 2020 Jun 27.

Epidemiology and Biostatistics Unit, Centre Armand-Frappier Santé Biotechnologie, Institut National de la Recherche Scientifique, University of Quebec, Laval, QC H7V 1B7, Canada.

This study describes the association between dietary patterns and prostate cancer (PCa) risk in a population-based case-control study conducted in Montreal, Canada (2005-2012). Cases ( = 1919) were histologically confirmed, aged ≤75 years. Concomitantly, controls ( = 1991) were randomly selected from the electoral list and frequency-matched to cases by age (±5 years). Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3390/nu12071907DOI Listing

Anti-diabetic and lipid-lowering effects of drimane sesquiterpenoids isolated from Zygogynum pancheri.

Chem Biol Interact 2020 Jun 27:109167. Epub 2020 Jun 27.

Laboratory of Organic Chemistry (LR17-ES08), Natural Substances Team, University of Sfax, Faculty of Sciences of Sfax, PB « 1171 », PC « 3000 », Sfax, Tunisia.

Recently, it has been shown that drimane-type sesquiterpenoids isolated from Zygogynum pancheri, a species native to New Caledonia, possessed significant α-amylase inhibitory activities. To further explore their antidiabetic potential, we investigated the effect of 1β-O-(E-cinnamoyl)-6α-hydroxy-9epi-polygodial (D) and 1β-E-O-p-methoxycinnamoyl-bemadienolide (L), two of the most active compounds of the series, on diabetic model rats. Compounds D and L (2 mg kg/day) were daily and orally administrated for 30 days to streptozotocin (STZ) (150 mg/kg) induced male diabetic Wistar rats. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.cbi.2020.109167DOI Listing

Characterization of the phenotypic and genotypic tolerance to abiotic stresses of natural populations of Heterorhabditis bacteriophora.

Sci Rep 2020 Jun 29;10(1):10500. Epub 2020 Jun 29.

Department of Entomology and the Nematology and Chemistry units, Plant Protection Institute; Agricultural Research Organization (ARO), the Volcani Center, Rishon Le Zion, Israel.

Entomopathogenic nematodes are effective biocontrol agents against arthropod pests. However, their efficacy is limited due to sensitivity to environmental extremes. The objective of the present study was to establish a foundation of genetic-based selection tools for beneficial traits of heat and desiccation tolerance in entomopathogenic nematodes. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41598-020-67097-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7324380PMC

Antimicrobial peptide LL-37 ameliorates a murine sepsis model via the induction of microvesicle release from neutrophils.

Innate Immun 2020 Jun 29:1753425920936754. Epub 2020 Jun 29.

Department of Host Defense and Biochemical Research, Juntendo University, Graduate School of Medicine, Japan.

Sepsis is a life-threatening disease caused by systemic dys-regulated inflammatory response to infection. We previously revealed that LL-37, a human cathelicidin antimicrobial peptide, improves the survival of cecal ligation and puncture septic mice. Ectosomes, microvesicles released from neutrophils, are reported to be elevated in sepsis survivors; however, the functions of ectosomes in sepsis remain largely unknown. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1177/1753425920936754DOI Listing

An Analysis of the Nutritional and Health Values of (Forsskål) and (Delile)-Two Chlorophyta Collected from the Philippines.

Molecules 2020 Jun 24;25(12). Epub 2020 Jun 24.

Laboratoire de Biotechnologie et Chimie Marines, EA3884, UBS, IUEM, F-56000 Vannes, France.

Polysaccharides, lipids and amino acid profiles were investigated to understand the nutritional value of and from the Philippines. The results revealed that both species contain high amounts of proteins (8.8-19. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3390/molecules25122901DOI Listing

Isocoumarin formation by heterologous gene expression and modification by host enzymes.

Org Biomol Chem 2020 Jun 26. Epub 2020 Jun 26.

Institut für Pharmazeutische Biologie und Biotechnologie, Philipps-Universität Marburg, Robert-Koch-Straße 4, 35037 Marburg, Germany.

Heterologous expression has been proven to be a successful strategy for the identification of metabolites encoded by cryptic/silent genes. Expression of a nonreducing polyketide synthase (NR-PKS) gene from Penicillium crustosum in Aspergillus nidulans led to the accumulation of three isocoumarins 1-3. Feeding experiments revealed that the PKS product 1 can be converted by the host enzymes to its hydroxylated (2) and methylated (3) derivatives. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1039/d0ob00989jDOI Listing

A colourimetric high-throughput screening system for directed evolution of prodigiosin ligase PigC.

Chem Commun (Camb) 2020 Jun 26. Epub 2020 Jun 26.

Lehrstuhl für Biotechnologie, RWTH Aachen University, Worringerweg 3, 52074 Aachen, Germany. and DWI-Leibniz Institut für Interaktive Materialien, Forckenbeckstraße 50, 52056 Aachen, Germany.

A colourimetric high-throughput screening system was established for directed evolution of prodigiosin ligase PigC. The two-step system consists of a colony prescreening test and a subsequent photometric 96-well plate assay. Screening PigC epPCR libraries in Pseudomonas putida revealed a PigC variant that achieved a 2. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1039/d0cc02181dDOI Listing

gen. nov., sp. nov., a new caproate-producing bacterium and emended description of the genus .

Int J Syst Evol Microbiol 2020 Jun 25. Epub 2020 Jun 25.

Institut für Mikrobiologie und Biotechnologie, Universität Ulm, Albert-Einstein-Allee 11, 89081 Ulm, Germany.

A strictly anaerobic bacterial strain designated EA1 was isolated from an enrichment culture inoculated with biogas reactor content. Cells of strain EA1 are spore-forming rods (1-3×0.4-0. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1099/ijsem.0.004283DOI Listing

Cationic Ru(II) cyclopentadienyl complexes with antifungal activity against several Candida species.

Chembiochem 2020 Jun 23. Epub 2020 Jun 23.

Institut national de la recherche scientifique, Institut Armand-Frappier, 531 boul. des Prairies, H7V 1B7, Laval, CANADA.

Fungal infections, including those caused by antifungal-resistant  Candida , are a very challenging health problem worldwide. Whereas different ruthenium complexes were previously studied for their anti- Candida  potential, Ru-cyclopentadienyl complexes were overlooked. Here, we report an antifungal activity assessment of three Ru-cyclopentadienyl complexes with some insights into their potential mode of action. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/cbic.202000254DOI Listing

Quantitative PCR assay for the simultaneous identification and enumeration of multiple Karenia species.

Environ Sci Pollut Res Int 2020 Jun 23. Epub 2020 Jun 23.

Laboratoire de Génie Enzymatique et Microbiologie, Equipe Biotechnologie des Algues, Ecole Nationale d'Ingénieurs de Sfax, Université de Sfax, Sfax, Tunisia.

Quantitative PCR (qPCR) is the method of choice for specific detection and quantification of harmful algal bloom (HAB) species. Development of qPCR assay for simultaneous enumeration of species that frequently co-exist in HABs is required. A high sensitivity TaqMan qPCR assay, using probe and primers, located at ITS1-5. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s11356-020-09739-4DOI Listing

Optimized qRT-PCR Approach for the Detection of Intra- and Extra-Cellular SARS-CoV-2 RNAs.

Int J Mol Sci 2020 Jun 20;21(12). Epub 2020 Jun 20.

Institute of Medical Virology, University Hospital Frankfurt am Main, Goethe University, 60590 Frankfurt am Main, Germany.

The novel coronavirus SARS-CoV-2 is the causative agent of the acute respiratory disease COVID-19, which has become a global concern due to its rapid spread. Meanwhile, increased demand for testing has led to a shortage of reagents and supplies and compromised the performance of diagnostic laboratories in many countries. Both the World Health Organization (WHO) and the Center for Disease Control and Prevention (CDC) recommend multi-step RT-PCR assays using multiple primer and probe pairs, which might complicate the interpretation of the test results, especially for borderline cases. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3390/ijms21124396DOI Listing

Assessment of the Performance of Electrodialysis in the Removal of the Most Potent Odor-Active Compounds of Herring Milt Hydrolysate: Focus on Ion-Exchange Membrane Fouling and Water Dissociation as Limiting Process Conditions.

Membranes (Basel) 2020 Jun 20;10(6). Epub 2020 Jun 20.

Department of Food Sciences, Université Laval, Québec, QC G1V 0A6, Canada.

Herring milt hydrolysate (HMH), like many fish products, presents the drawback to be associated with off-flavors. As odor is an important criterion, an effective deodorization method targeting the volatile compounds responsible for off-flavors needs to be developed. The potential of electrodialysis (ED) to remove the 15 volatile compounds identified, in the first part of this work, for their main contribution to the odor of HMH, as well as trimethylamine, dimethylamine and trimethylamine oxide, was assessed by testing the impact of both hydrolysate pH (4 and 7) and current conditions (no current vs. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3390/membranes10060127DOI Listing

[Protein arrays and perspectives of medical applications].

Ing Rech Biomed 2007 Nov-Dec;28(5):187-193. Epub 2008 Jul 28.

Biotechnologie, biocatalyse et biorégulation, UMR CNRS 6204, faculté des sciences et techniques, université de Nantes, 2, rue de la Houssinière, 44322 Nantes cedex 3 France.

Protein microarrays make it possible to detect molecular interactions with various partners (proteins, peptides, nucleic acids, sugars, etc.). Their advantages are crucial for high-throughput analysis of proteomes of different organisms. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.rbmret.2007.10.004DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7154616PMC

Intrinsic disorder in protein sense-antisense recognition.

J Mol Recognit 2020 Jun 22:e2868. Epub 2020 Jun 22.

Laboratory of New Methods in Biology, Institute for Biological Instrumentation of the Russian Academy of Sciences, Federal Research Center "Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences", Pushchino, Russia.

In addition to the well-established sense-antisense complementarity abundantly present in the nucleic acid world and serving as a basic principle of the specific double-helical structure of DNA, production of mRNA, and genetic code-based biosynthesis of proteins, sense-antisense complementarity is also present in proteins, where sense and antisense peptides were shown to interact with each other with increased probability. In nucleic acids, sense-antisense complementarity is achieved via the Watson-Crick complementarity of the base pairs or nucleotide pairing. In proteins, the complementarity between sense and antisense peptides depends on a specific hydropathic pattern, where codons for hydrophilic and hydrophobic amino acids in a sense peptide are complemented by the codons for hydrophobic and hydrophilic amino acids in its antisense counterpart. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/jmr.2868DOI Listing

Potential Hepatoprotective Activity of Super Critical Carbon Dioxide Olive Leaf Extracts against CCl-Induced Liver Damage.

Foods 2020 Jun 18;9(6). Epub 2020 Jun 18.

Research and Development Functional Food Centre (CIDAF), Health Science Technological Park, Avenida del Conocimiento 37, Edificio BioRegión, 18016 Granada, Spain.

Virgin olive oil has demonstrated its effective activity against oxidative stress. However, data on the bioactive effect of olive leaves or their major constituents on the liver are scarce. The present research work was conducted to evaluate the hepatoprotective effects of supercritical carbon dioxide (SC-CO) extracts from fresh and dried olive leaves on hepatotoxicity caused by carbon tetrachloride (CCl) in rat models. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3390/foods9060804DOI Listing

An automated and high-throughput-screening compatible pluripotent stem cell-based test platform for developmental and reproductive toxicity assessment of small molecule compounds.

Cell Biol Toxicol 2020 Jun 20. Epub 2020 Jun 20.

Fraunhofer IME ScreeningPort, Schnackenburgallee 114, 22525, Hamburg, Germany.

The embryonic stem cell test (EST) represents the only validated and accepted in vitro system for the detection and classification of compounds according to their developmental and reproductive teratogenic potency. The widespread implementation of the EST, however, in particular for routine application in pharmaceutical development, has not been achieved so far. Several drawbacks still limit the high-throughput screening of potential drug candidates in this format: The long assay period, the use of non-homogeneous viability assays, the low throughput analysis of marker protein expression and the compatibility of the assay procedures to automation. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s10565-020-09538-0DOI Listing

DEF6 deficiency, a mendelian susceptibility to EBV infection, lymphoma and autoimmunity.

J Allergy Clin Immunol 2020 Jun 17. Epub 2020 Jun 17.

Laboratory of Lymphocyte Activation and Susceptibility to EBV infection, Institut National de la Santé et de la Recherche Médicale (Inserm) UMR 1163, France; University of Paris, Imagine Institute, Paris, France. Electronic address:

By studying four siblings with a null homozygous mutation in DEF6, we show that DEF6 deficiency is a new inherited immune dysregulation disorder with variable expressivity from autoimmunity/inflammation to EBV-associated viremia/lymphoproliferation/lymphoma without extra-hematopoietic manifestations. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jaci.2020.05.052DOI Listing

Corrigendum: sp. nov., isolated from Saudi Arabian desert soil.

Int J Syst Evol Microbiol 2020 Apr;70(4):2955

Environmental Sciences Department, Faculty of Meteorology, Environment and Arid Land Agriculture, King Abdulaziz University, 21589 Jeddah, Saudi Arabia.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1099/ijsem.0.004039DOI Listing

Tranexamic acid quantification in human whole blood using liquid samples or volumetric absorptive microsampling devices.

Bioanalysis 2020 Jun 19. Epub 2020 Jun 19.

Département de Biotechnologie de la Santé, Université Paris-Saclay, UVSQ, INSERM, Infection et Inflammation, Montigny le Bretonneux, France.

Recent clinical trials demonstrate the benefits of the antifibrinolytic drug tranexamic acid but its pharmacokinetics remain to be investigated more in depth. Although pharmacokinetics studies are usually performed with plasma, volumetric absorptive microsampling devices allow us to analyze dried whole blood samples with several advantages. High-sensitivity LC-MS/MS methods for the quantification of tranexamic acid in human whole blood using liquid samples or dry samples on volumetric absorptive microsampling devices were developed and validated based on International Association from Therapeutic Drug Monitoring and Clinical Toxicology, European Medicines Agency and US Food and Drug Administration guidance. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.4155/bio-2020-0088DOI Listing

[Serum insulin and glucose concentrations in sheep during late pregnancy in relation to the number and sex of the fetuses].

Tierarztl Prax Ausg G Grosstiere Nutztiere 2020 Jun 18;48(3):141-147. Epub 2020 Jun 18.

Lehrstuhl für Biotechnologie der Tierreproduktion und für Umwelthygiene, Fakultät Biotechnologie und Tierzucht, Westpommersche Technologische Universität Szczecin.

Objective: The objective of this study was to investigate whether the serum concentration of glucose and insulin in the blood of pregnant sheep depends on the number and the sex of the fetuses.

Material And Methods: The presented research was carried out on Pomeranian (n = 42) and Suffolk sheep (n = 42) towards the end of the pregnancy. The following groups were established amongst the Pomeranian sheep: single pregnancies with a female fetuses (n = 19) and male fetuses (n = 10), twin pregnancies with female fetuses (n = 8), male fetuses (n = 8) and fetuses of different sex (n = 7). Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1055/a-1152-4157DOI Listing

Regulation of Gene expression at the neuromuscular Junction.

Neurosci Lett 2020 Jun 15;735:135163. Epub 2020 Jun 15.

INMG, Inserm U1217, CNRS UMR5310, Université Lyon 1, Université De Lyon, Lyon, France; Centre De Biotechnologie Cellulaire, Hospices Civils De Lyon, Lyon, France. Electronic address:

Gene expression in skeletal muscle is profoundly changed upon innervation. 50 years of research on the neuromuscular system have greatly increased our understanding of the mechanisms underlying these changes. By controlling the expression and the activity of key transcription factors, nerve-evoked electrical activity in the muscle fiber positively and negatively regulates the expression of hundreds of genes. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.neulet.2020.135163DOI Listing

Evaluation of Moroccan microalgae: Spirulina platensis as a potential source of natural antioxidants.

J Complement Integr Med 2020 Jun 9. Epub 2020 Jun 9.

Laboratoire Santé et Environnement, Département de biologie, Faculté des sciences - Ain Chock - Université Hassan II de Casablanca, B.P 5366 Mâarif, Casablanca, Morocco.

Background Ethanolic, aqueous and lipidic extracts of Spirulina platensis were evaluated for their bioactive substances (polyphenols and fatty acids) and antioxidant activities using two different assays diphenyl-1-picrylhydrazyl (DPPH) and azino-bis (ethylbenzthiazoline-6- sulfonicacid (ABTS)). Methods The phenolic compounds amounts were determined by colorimetric assays and were analyzed by high-performance and liquid chromatography (HPLC) method. The identification of phenolic compounds in the extract was accomplished by comparison of their retention times with those of pure standards. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1515/jcim-2019-0036DOI Listing

Insights on intermolecular FMN-heme domain interaction and the role of linker length in cytochrome P450cin fusion proteins.

Biol Chem 2020 Jun 1. Epub 2020 Jun 1.

Lehrstuhl für Biotechnologie, RWTH Aachen University, Worringerweg 3, D-52074 Aachen, Germany.

Cytochrome P450s are an important group of enzymes catalyzing hydroxylation and epoxidations reactions. In this work we describe the characterization of the CinA-CinC fusion enzyme system of a previously reported P450 using genetically fused heme (CinA) and FMN (CinC) enzyme domains from Citrobacter braaki. We observed that mixing individually inactivated heme (-) with FMN (-) domain in the CinA-10aa linker- CinC fusion constructs results in recovered activity and the formation of (2S)-2β-hydroxy,1,8-cineole (174 μM), a similar amount when compared to the fully functional fusion protein (176 μM). Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1515/hsz-2020-0134DOI Listing

Innate Immune Sensing by Cells of the Adaptive Immune System.

Front Immunol 2020 29;11:1081. Epub 2020 May 29.

INRS Centre Armand-Frappier Santé Biotechnologie, Laval, QC, Canada.

Sensing of microbes or of danger signals has mainly been attributed to myeloid innate immune cells. However, T and B cells also express functional pattern recognition receptors (PRRs). In these cells, PRRs mediate signaling cascades that result in different functions depending on the cell's activation and/or differentiation status, on the environment, and on the ligand/agonist. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3389/fimmu.2020.01081DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7274159PMC

A Gaseous Milieu: Extending the Boundaries of the Rhizosphere.

Trends Microbiol 2020 Jul 26;28(7):536-542. Epub 2020 Mar 26.

Institut National de la Recherche Scientifique, Centre Armand-Frappier Santé Biotechnologie, Laval, H7V 1B7, Canada; Quebec Center for Biodiversity Sciences (QCBS), Montreal, H3A 1B1, Canada.

Plant root activities shape microbial community functioning in the soil, making the rhizosphere the epicenter of soil biogeochemical processes. With this opinion article, we argue to rethink the rhizosphere boundaries: as gases can diffuse several centimeters away from the roots into the soil, the portion of soil influenced by root activities is larger than the strictly root-adhering soil. Indeed, gases are key drivers of biogeochemical processes due to their roles as energy sources or communication molecules, which has the potential to modify microbial community structure and functioning. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.tim.2020.02.016DOI Listing

Abasy Atlas v2.2: The most comprehensive and up-to-date inventory of meta-curated, historical, bacterial regulatory networks, their completeness and system-level characterization.

Comput Struct Biotechnol J 2020 16;18:1228-1237. Epub 2020 May 16.

Regulatory Systems Biology Research Group, Laboratory of Systems and Synthetic Biology, Center for Genomic Sciences, Universidad Nacional Autónoma de México, Av. Universidad s/n, Col. Chamilpa, 62210 Cuernavaca, Morelos, Mexico.

Some organism-specific databases about regulation in bacteria have become larger, accelerated by high-throughput methodologies, while others are no longer updated or accessible. Each database homogenize its datasets, giving rise to heterogeneity across databases. Such heterogeneity mainly encompasses different names for a gene and different network representations, generating duplicated interactions that could bias network analyses. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.csbj.2020.05.015DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7283102PMC

Development and Validation of the Reasons to Exergame (RTEX) Scale in Young Adults: Exploratory Factors Analysis.

JMIR Serious Games 2020 Jun 15;8(2):e16261. Epub 2020 Jun 15.

Department of Family Medicine, McGill University, Montreal, QC, Canada.

Background: Exergaming is associated with positive health benefits; however, little is known about what motivates young people to exergame.

Objective: This study aimed to develop a new Reasons to Exergame (RTEX) scale and describe its psychometric properties (Study 1) including test-retest reliability (Study 2). We also examined the test-retest reliability of self-report exergaming behavior measures (Study 2). Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.2196/16261DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7325003PMC

Generation of myogenic progenitor cell-derived smooth muscle cells for sphincter regeneration.

Stem Cell Res Ther 2020 Jun 12;11(1):233. Epub 2020 Jun 12.

Innovacell Biotechnologie AG, Mitterweg 24, 6020, Innsbruck, Austria.

Background: Degeneration of smooth muscles in sphincters can cause debilitating diseases such as fecal incontinence. Skeletal muscle-derived cells have been effectively used in clinics for the regeneration of the skeletal muscle sphincters, such as the external anal or urinary sphincter. However, little is known about the in vitro smooth muscle differentiation potential and in vivo regenerative potential of skeletal muscle-derived cells. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13287-020-01749-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7291744PMC

A new practical approach for the biological treatment of a mixture of cheese whey and white wastewaters using Kefir grains.

Environ Sci Pollut Res Int 2020 Jun 11. Epub 2020 Jun 11.

Laboratory of Treatment and Valorization of Water Rejects, Water Researches and Technologies Center, Borj-Cedria Technopark, University of Carthage, 8020, Soliman, Tunisia.

Kefir grains are a microbial consortium of different genera of bacteria and yeasts. In this study, the performance of Tunisian Kefir grains during the biological treatment of a mixture of Gouda cheese whey and white wastewaters (GCW) in ratio 1:1 with very high organic matter concentration is investigated. The biological process was evaluated and optimized through the response surface methodology. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s11356-020-09549-8DOI Listing

Identification of XylR, the Activator of Arabinose/Xylose Inducible Regulon in and Its Application for Homologous Protein Expression.

Front Microbiol 2020 26;11:1066. Epub 2020 May 26.

Institute of Biology II, Molecular Biology of Archaea, University of Freiburg, Freiburg, Germany.

The thermophilic archaeon can use different carbon sources for growth, including the pentoses D-xylose and L-arabinose. In this study, we identified the activator XylR () responsible for the transcriptional regulation of the pentose transporter and pentose metabolizing genes in . A deletion mutant showed growth retardation on D-xylose/L-arabinose containing media and the lack of transcription of the respective ABC transporter. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3389/fmicb.2020.01066DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7264815PMC

Engineered P450 BM3 and cpADH5 coupled cascade reaction for β-oxo fatty acid methyl ester production in whole cells.

Enzyme Microb Technol 2020 Aug 3;138:109555. Epub 2020 Apr 3.

Lehrstuhl für Biotechnologie, RWTH Aachen University, Worringerweg 3, 52074, Aachen, Germany; DWI-Leibniz Institut für Interaktive Materialien, Forckenbeckstraße 50, 52056, Aachen, Germany. Electronic address:

Hydroxy- or ketone- functionalized fatty acid methyl esters (FAMEs) are important compounds for production of pharmaceuticals, vitamins, cosmetics or dietary supplements. Biocatalysis through enzymatic cascades has drawn attention to the efficient, sustainable, and greener synthetic processes. Furthermore, whole cell catalysts offer important advantages such as cofactor regeneration by cell metabolism, omission of protein purification steps and increased enzyme stability. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.enzmictec.2020.109555DOI Listing

Genetic Structure of a Worldwide Germplasm Collection of L. Reveals Three Major Diffusion Routes for Varieties Coming From the Species' Center of Origin.

Front Plant Sci 2020 25;11:638. Epub 2020 May 25.

INRA Centre PACA, UR 1052 GAFL, Montfavet, France.

The characterization of the largest worldwide representative data set of apricot ( L.) germplasm was performed using molecular markers. Genetic diversity and structure of the cultivated apricot genetic resources were analyzed to decipher the history of diffusion of this species around the world. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3389/fpls.2020.00638DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7261834PMC

Frequency of genomic mutations mediating resistance of Mycobacterium tuberculosis isolates to rifampicin in Northern Morocco.

Rev Inst Med Trop Sao Paulo 2020 5;62:e37. Epub 2020 Jun 5.

Institut Pasteur du Maroc, Laboratoire de Biologie Moléculaire, Service de Recherche, Tanger, Morocco.

Drug resistant tuberculosis (DR-TB) is challenging particularly in developing countries. As such, a previous investigation gave the first insight into the mutational status of the Rifampicin Resistance Determining Region (RRDR) of rpoB gene among a restricted number of MTB patients' residents in the Northern Morocco. The purpose of this study was to investigate rpoB mutation types and frequencies associated with resistance to Rifampicin in a larger panel of MTB patients and to evaluate the usefulness of these mutations to improve the diagnosis of resistance to Rifampicin. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1590/S1678-9946202062037DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7274765PMC
June 2020
0.907 Impact Factor

Antioxidant, antidiabetic, and antihyperlipidemic activities of wheat flour-based chips incorporated with omega-3-rich fish oil and artichoke powder.

J Food Biochem 2020 Jun 9:e13297. Epub 2020 Jun 9.

Laboratoire des Substances Naturelles, Institut National de Recherche et d'Analyse Physico-chimique (INRAP), Biotechpôle de Sidi thabet, Ariana, Tunisia.

In the present study, the omega-3-rich oil from fish viscera and gill by-products, and caffeoylquinic-rich powder of artichoke bract by-products were used for the enrichment of wheat flour chips. Incorporation of these ingredients improved the lipid profile by increasing the level of polyunsaturated essential fatty acids mainly linoleic, linolenic, eicosapentaenoic, and docosahexaenoic acids enhancing thereby their nutritional quality. In alloxan-induced diabetic mice, the novel products reverts the blood glucose and serum markers including alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, urea, and creatinine to their normal levels. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/jfbc.13297DOI Listing
June 2020
0.741 Impact Factor

Distance-Based Metrics for Comparing Conformational Ensembles of Intrinsically Disordered Proteins.

Biophys J 2020 Jun 20;118(12):2952-2965. Epub 2020 May 20.

VIB-VUB Center for Structural Biology (CSB), Vlaams Instituut voor Biotechnologie, Brussels, Belgium; Structural Biology Brussels (SBB), Vrije Universiteit Brussel (VUB), Brussels, Belgium; Institute of Enzymology, Research Centre for Natural Sciences of the Hungarian Academy of Sciences, Budapest, Hungary. Electronic address:

Intrinsically disordered proteins are proteins whose native functional states represent ensembles of highly diverse conformations. Such ensembles are a challenge for quantitative structure comparisons because their conformational diversity precludes optimal superimposition of the atomic coordinates necessary for deriving common similarity measures such as the root mean-square deviation of these coordinates. Here, we introduce superimposition-free metrics that are based on computing matrices of the Cα-Cα distance distributions within ensembles and comparing these matrices between ensembles. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bpj.2020.05.015DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7300341PMC

Formaldehyde effects on kanamycin resistance gene of inactivated recombinant Escherichia coli vaccines.

Biotechnol Lett 2020 Jun 4. Epub 2020 Jun 4.

Centro de Desenvolvimento Tecnológico, Biotecnologia, Universidade Federal de Pelotas, Rio Grande do Sul, Pelotas, Brasil.

Objectives: Earlier studies have demonstrated the use of inactivated recombinant E. coli (bacterins), to protect against Clostridium spp. in vaccinated animals. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s10529-020-02929-7DOI Listing

Building Bridges between Biotechnology and Chemistry - Oreste Ghisalba's Pioneering Activities, Publications and Programs.

Chimia (Aarau) 2020 May;74(5):322-337

Swiss Coordination Committee on Biotechnology (SKB), Nordstrasse 2, CH-8021 Zurich, Switzerland; Lodz University of Technology, Institute of Molecular and Industrial Biotechnology, Stefanowskiego Street 4/10, 90-924 Lodz, Poland; , Email:

This contribution focusses on Oreste Ghisalba's pioneering activities in both fundamental as well as applied research in biocatalysis and his work on building bridges not only between biotechnology and chemistry, but also culturally, geographically and between academia and industry. His scientific work published in journals, books and conferences will be reviewed and his teaching at ETH Zurich and the University of Basel will be highlighted. Furthermore, an appreciation will be given of his broad knowledge and vision in shaping the activities of the Swiss Coordination Committee Biotechnology (SKB), the Swiss-Japanese Meetings in Biotechnology, conferences and research programs such as the Swiss Priority Program Biotechnology (SPP Biotech) of the Swiss National Science Foundation. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.2533/chimia.2020.322DOI Listing

Novel Fredericamycin Variant Overproduced by a Streptomycin-resistant subsp. Strain.

Mar Drugs 2020 May 28;18(6). Epub 2020 May 28.

Pharmazeutische Biotechnologie, Universität des Saarlandes, 66123 Saarbrücken, Germany.

Streptomycetes are an important source of natural products potentially applicable in the pharmaceutical industry. Many of these drugs are secondary metabolites whose biosynthetic genes are very often poorly expressed under laboratory cultivation conditions. In many cases, antibiotic-resistant mutants exhibit increased production of natural drugs, which facilitates the identification and isolation of new substances. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3390/md18060284DOI Listing

Translational profiling of macrophages infected with Leishmania donovani identifies mTOR- and eIF4A-sensitive immune-related transcripts.

PLoS Pathog 2020 Jun 1;16(6):e1008291. Epub 2020 Jun 1.

Institut National de la Recherche Scientifique (INRS)-Centre Armand-Frappier Santé Biotechnologie (CAFSB), Laval, Quebec, Canada.

The protozoan parasite Leishmania donovani (L. donovani) causes visceral leishmaniasis, a chronic infection which is fatal when untreated. Herein, we investigated whether in addition to altering transcription, L. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1371/journal.ppat.1008291DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7310862PMC

Characterization of an efficient extracellular cyanophycinase and its encoding cphE gene from Streptomyces pratensis strain YSM.

J Biotechnol 2020 Aug 27;319:15-24. Epub 2020 May 27.

Institut Für Molekulare Mikrobiologie Und Biotechnologie, Westfälische Wilhelms-Universität, Münster, Corrensstraße 3, 48149, Münster, Germany; Environmental Science Department, King Abdulaziz University, Jeddah, Saudi Arabia.

Until now, no enzymes were described that hydrolyze cyanophycin granular protein (CGP) from a species of the genus Streptomyces. An isolate able to hydrolyze CGP was identified as Streptomyces pratensis strain YSM. The CGPase from S. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jbiotec.2020.05.010DOI Listing

Dual-Reporting Transcriptionally Linked Genetically Encoded Fluorescent Indicators Resolve the Spatiotemporal Coordination of Cytosolic Abscisic Acid and Second Messenger Dynamics in Arabidopsis.

Plant Cell 2020 May 29. Epub 2020 May 29.

Heidelberg University CITY: Heidelberg POSTAL_CODE: 69120 Germany [DE].

Deciphering signal transduction processes is crucial for understanding how plants sense and respond to environmental changes. Various chemical compounds function as central messengers within deeply intertwined signaling networks. How such compounds act in concert remains to be elucidated. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1105/tpc.19.00892DOI Listing

Discovery of 2-aryl and 2-pyridinylbenzothiazoles endowed with antimicrobial and aryl hydrocarbon receptor agonistic activities.

Eur J Pharm Sci 2020 Aug 27;151:105386. Epub 2020 May 27.

Equipe de Chimie Moléculaire du Laboratoire Génomique, Bioinformatique et Chimie Moléculaire (EA 7528), Conservatoire National des Arts et Métiers (Cnam), 2 rue Conté, 75003, HESAM Université, Paris, France.. Electronic address:

Benzothiazole is a privileged scaffold in medicinal chemistry present in diverse bioactive compounds with multiple pharmacological applications such as analgesic, anticonvulsant, antidiabetic, anti-inflammatory, anticancer and radioactive amyloidal imagining agents. We reported in this work the study of sixteen functionalized 2-aryl and 2-pyridinylbenzothiazoles as antimicrobial agents and as aryl hydrocarbon receptor (AhR) modulators. The antimicrobial activity against Gram-positive (S. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ejps.2020.105386DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7251408PMC