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Biotechnologie Agronomie Societe Et Env... Publications | PubFacts

6,945 results match your criteria Biotechnologie Agronomie Societe Et Environnement[Journal]


Characterization and Dye Decolorization Potential of Two Laccases from the Marine-Derived Fungus sp

Int J Mol Sci 2019 Apr 15;20(8). Epub 2019 Apr 15.

Aix-Marseille Université, INRA UMR1163, Biodiversité et Biotechnologie Fongiques, 13288 Marseille, France.

Two laccase-encoding genes from the marine-derived fungus sp. have been cloned in for heterologous production, and the recombinant enzymes have been characterized to study their physicochemical properties, their ability to decolorize textile dyes for potential biotechnological applications, and their activity in the presence of sea salt. The optimal pH and temperature of Lac1 and Lac2 differed in relation to the substrates tested, and both enzymes were shown to be extremely stable at temperatures up to 50 °C, retaining 100% activity after 3 h at 50 °C. Read More

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http://dx.doi.org/10.3390/ijms20081864DOI Listing

"70 years of serotonin".

Biochimie 2019 Apr 12. Epub 2019 Apr 12.

Institut National de la recherche Scientifique (INRS), Centre Armand Frappier Santé Biotechnologie, 531 Boulevard des Prairies, Laval, Québec, H7V 1B7, Canada. Electronic address:

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http://dx.doi.org/10.1016/j.biochi.2019.04.012DOI Listing

Multi-Factorial-Guided Media Optimization for Enhanced Biomass and Lipid Formation by the Oleaginous Yeast .

Front Bioeng Biotechnol 2019 26;7:54. Epub 2019 Mar 26.

Werner Siemens-Lehrstuhl für Synthetische Biotechnologie, Technische Universität München, Garching, Germany.

The non-conventional, oleaginous yeast is flagged as an industrial cell factory for generation of oleochemicals and biofuels due to its substrate flexibility and high triglyceride yields. In this study, we employed a computational Response Surface Methodology to guide and streamline the experimental media optimization matrix with 12 nitrogen and 10 carbon sources in order to provide for high biomass and lipid accumulation toward an industrially relevant fermentation process. The resulting data provide new insights into physiology under variable nutritional states. Read More

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http://dx.doi.org/10.3389/fbioe.2019.00054DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6448043PMC

glyoxal oxidases display differential catalytic efficiencies on 5-hydroxymethylfurfural and its oxidized derivatives.

Fungal Biol Biotechnol 2019 1;6. Epub 2019 Apr 1.

1INRA, UMR1163 Biodiversité et Biotechnologie Fongiques (BBF), Aix Marseille Université, 13009 Marseille, France.

Background: 5-Hydroxymethylfurfural (HMF), a major residual component of a lignocellulosic bio-refinery process, can be transformed into fundamental building blocks for green chemistry via oxidation. While chemical methods are well established, interest is also being directed into the enzymatic oxidation of HMF into the bio-plastic precursor 2,5-furandicarboxylic acid (FDCA).

Results: We demonstrate that three glyoxal oxidases (GLOX) isoenzymes from the Basidiomycete fungus were able to oxidize HMF, with GLOX2 and GLOX3 being the most efficient. Read More

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http://dx.doi.org/10.1186/s40694-019-0067-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6442418PMC

Proteomics Reveal Enhanced Oxidative Stress Responses and Metabolic Adaptation in Biofilm Cells on Pyrite.

Front Microbiol 2019 29;10:592. Epub 2019 Mar 29.

Institute for Biological and Medical Engineering, Schools of Engineering, Medicine and Biological Sciences, Pontificia Universidad Católica de Chile, Santiago, Chile.

Reactive oxygen species (ROS) cause oxidative stress and growth inhibition by inactivation of essential enzymes, DNA and lipid damage in microbial cells. Acid mine drainage (AMD) ecosystems are characterized by low pH values, enhanced levels of metal ions and low species abundance. Furthermore, metal sulfides, such as pyrite and chalcopyrite, generate extracellular ROS upon exposure to acidic water. Read More

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http://dx.doi.org/10.3389/fmicb.2019.00592DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6450195PMC

Bisphenol A differentially affects male reproductive function biomarkers in a reference population and agro pesticides users from Djutitsa, Cameroon.

Toxicol Ind Health 2019 Apr;35(4):324-335

6 Laboratory of Pharmacology and Toxicology, Department of Biochemistry, Faculty of Science, University of Yaoundé I, Yaoundé, Cameroon.

The consequences of bisphenol A (BPA) exposure on male reproductive function were studied in two populations from Cameroon, farmers using agro pesticides in Djutitsa (rural area) and townsmen in Yaoundé (urban area, Centre region). Urinary BPA concentration from all participants was measured, and the values were correlated with biochemical markers of male reproductive function. The data showed that BPA could be detected in 92. Read More

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http://dx.doi.org/10.1177/0748233719838437DOI Listing

Phylogenetic Studies.

Methods Mol Biol 2019 ;1971:9-68

Global Health and Tropical Medicine (GHTM), Instituto de Higiene e Medicina Tropical (IHMT), Universidade Nova de Lisboa (UNL), Lisbon, Portugal.

Phylogenetics is an important component of the systems biology approach. Knowledge about evolution of the genus Leishmania is essential to understand various aspects of basic biology of these parasites, such as parasite-host or parasite-vector relationships, biogeography, or epidemiology. Here, we present a comprehensive guideline for performing phylogenetic studies based on DNA sequence data, but with principles that can be adapted to protein sequences or other molecular markers. Read More

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http://dx.doi.org/10.1007/978-1-4939-9210-2_2DOI Listing
January 2019

Thermodynamic Stability of the Transcription Regulator PaaR2 from Escherichia coli O157:H7.

Biophys J 2019 Mar 28. Epub 2019 Mar 28.

Structural Biology Brussels, Department of Biotechnology, Vrije Universiteit Brussel, Brussels, Belgium; Center for Structural Biology, Vlaams Instituut voor Biotechnologie, Brussels, Belgium. Electronic address:

PaaR2 is a putative transcription regulator encoded by a three-component parDE-like toxin-antitoxin module from Escherichia coli O157:H7. Although this module's toxin, antitoxin, and toxin-antitoxin complex have been more thoroughly investigated, little remains known about its transcription regulator PaaR2. Using a wide range of biophysical techniques (circular dichroism spectroscopy, size-exclusion chromatography-multiangle laser light scattering, dynamic light scattering, small-angle x-ray scattering, and native mass spectrometry), we demonstrate that PaaR2 mainly consists of α-helices and displays a concentration-dependent octameric build-up in solution and that this octamer contains a global shape that is significantly nonspherical. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00063495193020
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http://dx.doi.org/10.1016/j.bpj.2019.03.015DOI Listing
March 2019
1 Read

Tracking of enzymatic biomass deconstruction by fungal secretomes highlights markers of lignocellulose recalcitrance.

Biotechnol Biofuels 2019 1;12:76. Epub 2019 Apr 1.

2INRA, Aix Marseille Univ., UMR1163, BBF, Biodiversité et Biotechnologie Fongiques, Marseille, France.

Background: Lignocellulose biomass is known as a recalcitrant material towards enzymatic hydrolysis, increasing the process cost in biorefinery. In nature, filamentous fungi naturally degrade lignocellulose, using an arsenal of hydrolytic and oxidative enzymes. Assessment of enzyme hydrolysis efficiency generally relies on the yield of glucose for a given biomass. Read More

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http://dx.doi.org/10.1186/s13068-019-1417-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6442405PMC
April 2019
1 Read

Activity of Thymus capitatus essential oil components against in vitro cultured Echinococcus multilocularis metacestodes and germinal layer cells.

Parasitology 2019 Apr 12:1-12. Epub 2019 Apr 12.

Institute of Parasitology, Vetsuisse Faculty, University of Berne,CH-3012 Berne,Switzerland.

The essential oil (EO) of Thymus capitatus, seven fractions (F1-F7) obtained from silica gel chromatography, and several pure EO components were evaluated with respect to in vitro activities against Echinococcus multilocularis metacestodes and germinal layer (GL) cells. Attempts to evaluate physical damage in metacestodes by phosphoglucose isomerase (PGI) assay failed because EO and F1-F7 interfered with the PGI-activity measurements. A metacestode viability assay based on Alamar Blue, as well as transmission electron microscopy, demonstrated that exposure to EO, F2 and F4 impaired metacestode viability. Read More

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http://dx.doi.org/10.1017/S0031182019000295DOI Listing

Targeting Crf Transglycosylases With Neutralizing Antibody Is Relevant but Not Sufficient to Erase Fungal Burden in a Neutropenic Rat Model.

Front Microbiol 2019 26;10:600. Epub 2019 Mar 26.

INSERM, Centre d'Étude des Pathologies Respiratoires, U1100, Tours, France.

is an airborne opportunistic fungal pathogen responsible for severe infections. Among them, invasive pulmonary aspergillosis has become a major concern as mortality rates exceed 50% in immunocompromised hosts. In parallel, allergic bronchopulmonary aspergillosis frequently encountered in cystic fibrosis patients, is also a comorbidity factor. Read More

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http://dx.doi.org/10.3389/fmicb.2019.00600DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6443627PMC
March 2019
1 Read

Non-Leachable Hydrophilic Additives for Amphiphilic Coatings.

Polymers (Basel) 2018 Apr 16;10(4). Epub 2018 Apr 16.

University of Southern Brittany, EA 3884, Laboratoire de Biotechnologie et Chimie Marines (LBCM), Institut Universitaire Européen de la Mer IUEM, F-56100 Lorient, France.

Amphiphilic surfaces are particularly effective at inhibiting the adhesion of microorganisms (bacteria, cells, microalgae, etc.) in liquid media. The aim of this study is to determine the best hydrophilic linker to promote bonding between poly(ethylene glycol) (PEG) as a hydrophilic additive and poly(dimethyl siloxane) (PDMS) as the hydrophobic matrix. Read More

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http://dx.doi.org/10.3390/polym10040445DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6415241PMC

The Ca2+ sensor SCaBP3/CBL7 Fine Tunes Arabidopsis Alkali Tolerance and Modulats Plasma Membrane H+-ATPase Activity.

Plant Cell 2019 Apr 8. Epub 2019 Apr 8.

China Agricultural University CITY: Beijing POSTAL_CODE: n/a China [CN]

Saline-alkali soil is a major environmental constraint impairing plant growth and crop productivity. In this study, we identify a Ca2+ sensor/kinase/plasma membrane H+-ATPase module as central component conferring alkali tolerance in Arabidopsis. We report that SCaBP3/CBL7 loss-of-function plant exhibits enhanced stress tolerance associated with increased PM H+-ATPase activity and provides fundamental mechanistic insights into the regulation of PM H+-ATPase activity. Read More

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http://dx.doi.org/10.1105/tpc.18.00568DOI Listing

Seasonal and pharmaceutical-induced changes in selenoprotein glutathione peroxidase 4 activity in the reproductive dynamics of the soil biosentinel Podarcis sicula (Chordata: Reptilia).

Mol Reprod Dev 2019 Apr 7. Epub 2019 Apr 7.

Department of Chemical Sciences, University of Naples Federico II, Complesso Universitario di Monte S. Angelo, Naples, Italy.

There is rising concern for the interaction of environmental contaminants with brain transcriptome and the potential effect on reproductive processes. The present study sought to determine selenoprotein glutathione peroxidase 4 (gpx4) transcriptional activity in the brain and testis of the soil biosentinel, Podarcis sicula, through the main phases of the reproductive cycle and whether pharmaceuticals exert an endocrine disruption. Based on gpx4 cloned amminoacids sequence (GenBank AEX09236. Read More

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http://dx.doi.org/10.1002/mrd.23143DOI Listing
April 2019
3 Reads

ZEBs: Novel Players in Immune Cell Development and Function.

Trends Immunol 2019 Apr 4. Epub 2019 Apr 4.

Division of Biological Sciences, University of California, San Diego, La Jolla, CA, USA. Electronic address:

ZEB1 and ZEB2 are zinc-finger E homeobox-binding transcription factors best known for their role in driving epithelial to mesenchymal transition. However, in recent years our understanding of these two transcription factors has broadened, and it is now clear that they are expressed by a variety of immune cells of both myeloid and lymphoid lineages, including dendritic cells, macrophages, monocytes, B, T, and NK cells. In these cells, ZEBs function to regulate important transcriptional networks necessary for cell differentiation, maintenance, and function. Read More

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http://dx.doi.org/10.1016/j.it.2019.03.001DOI Listing

Chemical validation of DegS as a target for the development of antibiotics with a novel mode of action.

ChemMedChem 2019 Apr 4. Epub 2019 Apr 4.

Universität Duisburg-Essen, Zentrum für Medizinische Biotechnologie, Universitätsstr. 3, 45117, Essen, GERMANY.

Despite the availability of hundreds of antibiotic drugs, infectious diseases continue to remain one of the most notorious health issues. In addition, the disparity between the spreading of multidrug resistant pathogens and the development of novel classes of antibiotics exemplify an important unmet medical need that can only be addressed by identifying novel targets. Here we demonstrate by the development of the first in vivo active DegS inhibitors based on a pyrazolo[1,5-a]-1,3,5-triazine scaffold that DegS and the cell envelope stress response pathway sigmaE represent a target for generating antibiotics with a novel mode-of-action. Read More

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http://dx.doi.org/10.1002/cmdc.201900193DOI Listing

Structures of ligand-occupied β-Klotho complexes reveal a molecular mechanism underlying endocrine FGF specificity and activity.

Proc Natl Acad Sci U S A 2019 Apr 3;116(16):7819-7824. Epub 2019 Apr 3.

Department of Pharmacology, Yale University School of Medicine, New Haven, CT 06520;

The three members of the endocrine fibroblast growth factor (FGF) family designated FGF19, FGF21, and FGF23 mediate their pleiotropic cellular effects by binding to and activating binary complexes composed of an FGF receptor (FGFR) bound to either α-Klotho or β-Klotho receptors. Structural analyses of ligand-occupied Klotho extracellular domains have provided important insights concerning mechanisms underlying the binding specificities of FGF21 and FGF23 to β-Klotho or α-Klotho, respectively. They have also demonstrated that Klotho proteins function as primary high-affinity receptors while FGFRs function as the catalytic subunits that mediate intracellular signaling. Read More

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http://dx.doi.org/10.1073/pnas.1822055116DOI Listing
April 2019
2 Reads

Differential effects of the Akt inhibitor MK-2206 on migration and radiation sensitivity of glioblastoma cells.

BMC Cancer 2019 Apr 3;19(1):299. Epub 2019 Apr 3.

Department of Biotechnology and Biophysics, University of Würzburg, 97074, Würzburg, Germany.

Background: Most tumor cells show aberrantly activated Akt which leads to increased cell survival and resistance to cancer radiotherapy. Therefore, targeting Akt can be a promising strategy for radiosensitization. Here, we explore the impact of the Akt inhibitor MK-2206 alone and in combination with the dual PI3K and mTOR inhibitor PI-103 on the radiation sensitivity of glioblastoma cells. Read More

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http://dx.doi.org/10.1186/s12885-019-5517-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6446411PMC
April 2019
1 Read

Enhancing the synthesis of latex clearing protein by different cultivation strategies.

J Biotechnol 2019 Mar 30;297:32-40. Epub 2019 Mar 30.

Institut für Molekulare Mikrobiologie und Biotechnologie, Westfälische Wilhelms-Universität Münster, D-48149, Münster, Germany.

In this study, we improved the synthesis of the latex clearing protein from Gordonia polyisoprenivorans VH2 (Lcp1), a key enzyme for the initial cleavage of the rubber backbone. Cultivations using a recombinant strain of Escherichia coli were optimized to overcome poor solubility of Lcp1 and improve the production yields. Different cultivation temperatures and agitation rates were evaluated in the process to demonstrate their impact on the solubility of Lcp1. Read More

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http://dx.doi.org/10.1016/j.jbiotec.2019.03.019DOI Listing

Re-evaluation of cyanophycin synthesis in Corynebacterium glutamicum and incorporation of glutamic acid and lysine into the polymer.

Appl Microbiol Biotechnol 2019 Apr 1. Epub 2019 Apr 1.

Institut für Molekulare Mikrobiologie und Biotechnologie, Westfälische Wilhelms-Universität, Münster, Corrensstraße 3, 48149, Münster, Germany.

Corynebacterium glutamicum was only examined in the early 2000s as a possible microorganism for the production of the polyamide cyanophycin (multi-L-arginyl-poly-[L-aspartic acid], CGP). CGP is a potential precursor for the synthesis of polyaspartic acid and CGP-derived dipeptides which may be of use in peptide-based clinical diets, as dietary supplements, or in livestock feeds. In the past, C. Read More

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http://dx.doi.org/10.1007/s00253-019-09780-5DOI Listing
April 2019
2 Reads

A Review of the Construction of Nano-Hybrids for Electrochemical Biosensing of Glucose.

Biosensors (Basel) 2019 Mar 25;9(1). Epub 2019 Mar 25.

BAE: Biocapteurs-Analyses-Environnement, Universite de Perpignan ViaDomitia, 52 Avenue Paul Alduy, 66860 Perpignan CEDEX, France.

Continuous progress in the domain of nano and material science has led to modulation of the properties of nanomaterials in a controlled and desired fashion. In this sense, nanomaterials, including carbon-based materials, metals and metal oxides, and composite/hybrid materials have attracted extensive interest with regard to the construction of electrochemical biosensors. The modification of a working electrode with a combination of two or three nanomaterials in the form of nano-composite/nano-hybrids has revealed good results with very good reproducibility, stability, and improved sensitivity. Read More

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http://dx.doi.org/10.3390/bios9010046DOI Listing

Spontaneous driving forces give rise to protein-RNA condensates with coexisting phases and complex material properties.

Proc Natl Acad Sci U S A 2019 Apr 29;116(16):7889-7898. Epub 2019 Mar 29.

Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305;

Phase separation of multivalent protein and RNA molecules underlies the biogenesis of biomolecular condensates such as membraneless organelles. In vivo, these condensates encompass hundreds of distinct types of molecules that typically organize into multilayered structures supporting the differential partitioning of molecules into distinct regions with distinct material properties. The interplay between driven (active) versus spontaneous (passive) processes that are required for enabling the formation of condensates with coexisting layers of distinct material properties remains unclear. Read More

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http://dx.doi.org/10.1073/pnas.1821038116DOI Listing

AA16, a new lytic polysaccharide monooxygenase family identified in fungal secretomes.

Biotechnol Biofuels 2019 16;12:55. Epub 2019 Mar 16.

1Biodiversité et Biotechnologie Fongiques, UMR1163, INRA, Aix Marseille Université, Marseille, France.

Background: Lignocellulosic biomass is considered as a promising alternative to fossil resources for the production of fuels, materials and chemicals. Efficient enzymatic systems are needed to degrade the plant cell wall and overcome its recalcitrance. A widely used producer of cellulolytic cocktails is the ascomycete , but this organism secretes a limited set of enzymes. Read More

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http://dx.doi.org/10.1186/s13068-019-1394-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6420742PMC

Modeling of Reactive Black 5 decolorization in presence of heavy metals by the newly isolated Pseudomonas aeruginosa strain Gb30.

J Appl Microbiol 2019 Mar 27. Epub 2019 Mar 27.

Laboratory of Enzyme Engineering and Microbiology, National School of Engineers of Sfax, University of Sfax, BP 1173, 3038, Sfax, Tunisia.

Aim: The effect of heavy metals presence on the decolorization of Reactive Black 5 by Pseudomonas aeruginosa was evaluated.

Methods And Results: In the current study, a newly isolated strain identified as P. aeruginosa strain Gb 30 was selected for its ability to remove high concentration of Reactive Black 5 and resistance to several heavy metals (Cu >Zn >Cd >Cr ). Read More

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http://dx.doi.org/10.1111/jam.14262DOI Listing

Navigating metabolic pathways to enhance antitumour immunity and immunotherapy.

Nat Rev Clin Oncol 2019 Mar 26. Epub 2019 Mar 26.

Department of Fundamental Oncology, University of Lausanne, Lausanne, Vaud, Switzerland.

The development of immunotherapies over the past decade has resulted in a paradigm shift in the treatment of cancer. However, the majority of patients do not benefit from immunotherapy, presumably owing to insufficient reprogramming of the immunosuppressive tumour microenvironment (TME) and thus limited reinvigoration of antitumour immunity. Various metabolic machineries and nutrient-sensing mechanisms orchestrate the behaviour of immune cells in response to nutrient availability in the TME. Read More

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http://dx.doi.org/10.1038/s41571-019-0203-7DOI Listing
March 2019
3 Reads

Sucrose 6-phosphate phosphorylase: a novel insight in the human gut microbiome.

Microb Genom 2019 Mar 26. Epub 2019 Mar 26.

1​Aix Marseille Univ, CNRS, Centrale Marseille, iSm2, Marseille, France.

The human gut microbiome plays an essential role in maintaining human health including in degradation of dietary fibres and carbohydrates further used as nutrients by both the host and the gut bacteria. Previously, we identified a polysaccharide utilization loci (PUL) involved in sucrose and raffinose family oligosaccharide (RFO) metabolism from one of the most common Firmicutes present in individuals, Ruminococcus gnavus E1. One of the enzymes encoded by this PUL was annotated as a putative sucrose phosphate phosphorylase (RgSPP). Read More

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http://dx.doi.org/10.1099/mgen.0.000253DOI Listing
March 2019
2 Reads

New species of pathogenic Pseudomonas isolated from citrus in Tunisia: Proposal of Pseudomonas kairouanensis sp. nov. and Pseudomonas nabeulensis sp. nov.

Syst Appl Microbiol 2019 Mar 15. Epub 2019 Mar 15.

Microbiologia, Departament de Biologia, Edifici Guillem Colom, Universitat de les Illes Balears, Campus UIB, 07122 Palma de Mallorca, Spain; Institut Mediterrani d'Estudis Avançats (IMEDEA, CSIC-UIB), Campus UIB, 07122 Palma de Mallorca, Spain. Electronic address:

A collection of Pseudomonas strains was isolated in different regions of Tunisia in the period 2016-2017 from the fruits and leaves of Citrus sinensis cv. 'Valencia Late' and Citrus limon cv. 'Eureka' plants with symptoms of blast and black pit disease. Read More

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http://dx.doi.org/10.1016/j.syapm.2019.03.002DOI Listing

Antibacterial and Biofilm Inhibitory Activity of Medicinal Plant Essential Oils Against Isolated from UTI Patients.

Molecules 2019 Mar 23;24(6). Epub 2019 Mar 23.

Department of Biology, Faculty of Science, Taif University, Taif 11099, Saudi Arabia.

Urinary tract infections (UTIs), caused by 80% to 85% of the time, are one of the most important causes of morbidity and health care spending affecting persons of all ages. These infections lead to many difficult problems, especially increasing resistance to antibiotic drugs. Bacterial biofilms play an important role in UTIs, responsible for persistent infections leading to recurrences and relapses. Read More

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http://dx.doi.org/10.3390/molecules24061161DOI Listing

An Uncommon Type II PKS Catalyzes Biosynthesis of Aryl Polyene Pigments.

J Am Chem Soc 2019 Apr 3. Epub 2019 Apr 3.

Molekulare Biotechnologie, Fachbereich Biowissenschaften, Goethe-Universität Frankfurt am Main and Buchmann Institute for Molecular Life Sciences (BMLS) , Goethe-Universität Frankfurt , Max-von-Laue-Straße 9 and 15 , 60438 Frankfurt am Main , Germany.

Aryl polyene (APE) pigments are a widely distributed class of bacterial polyketides. So far, little is known about the biosynthesis of these compounds, which are produced by a novel type II polyketide synthase (PKS). We have identified all enzymes involved in APE biosynthesis and determined their peculiar functions. Read More

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http://dx.doi.org/10.1021/jacs.8b10776DOI Listing
April 2019
1 Read

Functional Outcome of Intravenous Thrombolysis in Patients With Lacunar Infarcts in the WAKE-UP Trial.

JAMA Neurol 2019 Mar 25. Epub 2019 Mar 25.

Klinik und Poliklinik für Neurologie, Kopf- und Neurozentrum, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.

Importance: The rationale for intravenous thrombolysis in patients with lacunar infarcts is debated, since it is hypothesized that the microvascular occlusion underlying lacunar infarcts might not be susceptible to pharmacological reperfusion treatment.

Objective: To study the efficacy and safety of intravenous thrombolysis among patients with lacunar infarcts.

Design, Setting, And Participants: This exploratory secondary post hoc analysis of the WAKE-UP trial included patients who were screened and enrolled between September 2012 and June 2017 (with final follow-up in September 2017). Read More

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http://dx.doi.org/10.1001/jamaneurol.2019.0351DOI Listing
March 2019
1 Read

A 3-variable PDE model for predicting fungal growth derived from microscopic mechanisms.

J Theor Biol 2019 Jun 21;470:90-100. Epub 2019 Mar 21.

LGPM, CentraleSupélec, SFR Condorcet FR CNRS 3417, Centre Européen de Biotechnologie et de Bioéconomie (CEBB), Université Paris-Saclay, 3 Rue des Rouges Terres, 51110, Pomacle, France; LGPM, CentraleSupélec, Université Paris-Saclay, 8-10 Rue Joliot-Curie, 91190, Gif-sur-Yvette, France. Electronic address:

In this work, we present a new PDE model of the growth of Postia placenta, a species of brown rot fungus. The formulation was derived mainly from the biological mechanisms embedded in our discrete model, validated against experimental data. In order to mimic the growth mechanisms, we propose a new reaction-diffusion formulation, based on three variables: the concentration of tips, the branch density and the total hyphal density. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00225193193012
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http://dx.doi.org/10.1016/j.jtbi.2019.03.015DOI Listing
June 2019
7 Reads

Broad-specificity GH131 β-glucanases are a hallmark of Fungi and Oomycetes that colonise plants.

Environ Microbiol 2019 Mar 18. Epub 2019 Mar 18.

INRA, Aix-Marseille Univ, UMR1163, Biodiversité et Biotechnologie Fongiques, BBF, Marseille, France.

Plant-tissue-colonising fungi fine-tune the deconstruction of plant-cell walls (PCW) using different sets of enzymes according to their lifestyle. However, some of these enzymes are conserved among fungi with dissimilar lifestyles. We identified genes from Glycoside Hydrolase family GH131 as commonly expressed during plant-tissue colonisation by saprobic, pathogenic, and symbiotic fungi. Read More

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http://dx.doi.org/10.1111/1462-2920.14596DOI Listing
March 2019
1 Read

Dual phenazine gene clusters enable diversification during biosynthesis.

Nat Chem Biol 2019 04 18;15(4):331-339. Epub 2019 Mar 18.

Molekulare Biotechnologie, Fachbereich Biowissenschaften, Goethe Universität Frankfurt, Frankfurt am Main, Germany.

Biosynthetic gene clusters (BGCs) bridging genotype and phenotype continuously evolve through gene mutations and recombinations to generate chemical diversity. Phenazine BGCs are widespread in bacteria, and the biosynthetic mechanisms of the formation of the phenazine structural core have been illuminated in the last decade. However, little is known about the complex phenazine core-modification machinery. Read More

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http://dx.doi.org/10.1038/s41589-019-0246-1DOI Listing

Chemical Composition and Acaricidal Activity of the Essential Oils of Some Plant Species of and against the Vector of Tropical Bovine Theileriosis: (syn. ).

Biomed Res Int 2019 7;2019:7805467. Epub 2019 Feb 7.

Institute of Veterinary Science, University Chadli Bendjedid El-Tarf, BP 73, El-Tarf 36000, Algeria.

The present study aimed to investigate the acaricidal properties of six essential oils. They were extracted from some plant species ( and ) using the technique of hydrodistillation with the Clevenger apparatus. The chemical compositions of the essential oils under study were determined by gas chromatography-mass spectrometer (GC-MS). Read More

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http://dx.doi.org/10.1155/2019/7805467DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6383433PMC
February 2019

The Balancing Act of Intrinsically Disordered Proteins: Enabling Functional Diversity while Minimizing Promiscuity.

J Mol Biol 2019 Apr 13;431(8):1650-1670. Epub 2019 Mar 13.

VIB-VUB Center for Structural Biology, Vlaams Instituut voor Biotechnologie, Pleinlaan 2, 1050 Brussels, Belgium. Electronic address:

Intrinsically disordered proteins (IDPs) or regions (IDRs) perform diverse cellular functions, but are also prone to forming promiscuous and potentially deleterious interactions. We investigate the extent to which the properties of, and content in, IDRs have adapted to enable functional diversity while limiting interference from promiscuous interactions in the crowded cellular environment. Information on protein sequences, their predicted intrinsic disorder, and 3D structure contents is related to data on protein cellular concentrations, gene co-expression, and protein-protein interactions in the well-studied yeast Saccharomyces cerevisiae. Read More

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http://dx.doi.org/10.1016/j.jmb.2019.03.008DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6453724PMC
April 2019
3 Reads

Structural and functional analysis of glycoside hydrolase 138 enzymes targeting chain A galacturonic acid in the complex pectin rhamnogalacturonan II.

J Biol Chem 2019 Mar 15. Epub 2019 Mar 15.

Newcastle University, United Kingdom.

The metabolism of carbohydrate polymers drives microbial diversity in the human gut microbiome. The selection pressures in this environment have spurred the evolution of a complex reservoir of microbial genes encoding carbohydrate-active enzymes (CAZymes). Previously, we have shown that the human gut bacterium () can depolymerize the most structurally complex glycan, the plant pectin rhamnogalacturonan II (RGII), commonly found in the human diet. Read More

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http://dx.doi.org/10.1074/jbc.RA118.006626DOI Listing
March 2019
4 Reads

Synthesis and SAR of the antistaphylococcal natural product nematophin from .

Beilstein J Org Chem 2019 25;15:535-541. Epub 2019 Feb 25.

Molekulare Biotechnologie, Goethe University Frankfurt, Max-von-Laue-Str. 9, D-60438 Frankfurt am Main, Germany.

The repeated and improper use of antibiotics had led to an increased number of multiresistant bacteria. Therefore, new lead structures are needed. Here, the synthesis and an expanded structure-activity relationship of the simple and antistaphylococcal amide nematophin from and synthetic derivatives are described. Read More

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http://dx.doi.org/10.3762/bjoc.15.47DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6404511PMC
February 2019

Cytological, Biochemical and Molecular Events of the Embryogenic State in Douglas-fir ( [Mirb.]).

Front Plant Sci 2019 28;10:118. Epub 2019 Feb 28.

BioForA, INRA, ONF, Orléans, France.

Somatic embryogenesis techniques have been developed for most coniferous species, but only using very juvenile material. To extend the techniques' scope, better integrated understanding of the key biological, physiological and molecular characteristics of embryogenic state is required. Therefore, embryonal masses (EMs) and non-embryogenic calli (NECs) have been compared during proliferation at multiple levels. Read More

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http://dx.doi.org/10.3389/fpls.2019.00118DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6403139PMC
February 2019
1 Read

Uniform Widespread Nuclear Phosphorylation of Histone H2AX Is an Indicator of Lethal DNA Replication Stress.

Cancers (Basel) 2019 Mar 13;11(3). Epub 2019 Mar 13.

Biotechnologie et Signalisation Cellulaire, UMR 7242, CNRS/Université de Strasbourg, Boulevard S. Brant, 67412 Illlkirch, France.

Phosphorylated histone H2AX (γ-H2AX), a central player in the DNA damage response (DDR), serves as a biomarker of DNA double-strand break repair. Although DNA damage is generally visualized by the formation of γ-H2AX foci in injured nuclei, it is unclear whether the widespread uniform nuclear γ-H2AX (called pan-nuclear) pattern occurring upon intense replication stress (RS) is linked to DDR. Using a novel monoclonal antibody that binds exclusively to the phosphorylated C-terminus of H2AX, we demonstrate that H2AX phosphorylation is systematically pan-nuclear in cancer cells stressed with RS-inducing drugs just before they die. Read More

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http://dx.doi.org/10.3390/cancers11030355DOI Listing
March 2019
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Different behaviors of cyclic dipeptide prenyltransferases toward the tripeptide derivative ardeemin fumiquinazoline and its enantiomer.

Appl Microbiol Biotechnol 2019 May 12;103(9):3773-3781. Epub 2019 Mar 12.

Institut für Pharmazeutische Biologie und Biotechnologie, Philipps-Universität Marburg, Robert-Koch-Str. 4, 35037, Marburg, Germany.

In nature, cyclic dipeptide prenyltransferases catalyze regioselective Friedel-Crafts alkylations of tryptophan-containing cyclic dipeptides. This enzyme class, belonging to the dimethylallyl tryptophan synthase superfamily, is known to be flexible toward aromatic prenyl acceptors, while mostly retaining its typical regioselectivity. Ardeemin fumiquinazoline (FQ) (1), a tryptophan-containing cyclic tripeptide derivative, is assembled in Aspergillus fischeri by the non-ribosomal peptide synthetase ArdA and modified by the prenyltransferase ArdB, leading to the pharmaceutically active hexacyclic ardeemin. Read More

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http://link.springer.com/10.1007/s00253-019-09723-0
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http://dx.doi.org/10.1007/s00253-019-09723-0DOI Listing
May 2019
3 Reads

The SOS2-SCaBP8 Complex Generates and Fine-Tunes an AtANN4-Dependent Calcium Signature under Salt Stress.

Dev Cell 2019 Mar;48(5):697-709.e5

State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100193, China. Electronic address:

Calcium signals act as universal second messengers that trigger many cellular processes in animals and plants, but how specific calcium signals are generated is not well understood. In this study, we determined that AtANN4, a putative calcium-permeable transporter, and its interacting proteins, SCaBP8 and SOS2, generate a calcium signal under salt stress, which initially activates the SOS pathway, a conserved mechanism that modulates ion homeostasis in plants under salt stress. After activation, SCaBP8 promotes the interaction of protein kinase SOS2 with AtANN4, which enhances its phosphorylation by SOS2. Read More

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http://dx.doi.org/10.1016/j.devcel.2019.02.010DOI Listing
March 2019
44 Reads

Ailanthus altissima (Miller) Swingle seed oil: chromatographic characterization by GC-FID and HS-SPME-GC-MS, physicochemical parameters, and pharmacological bioactivities.

Environ Sci Pollut Res Int 2019 Mar 11. Epub 2019 Mar 11.

Laboratory of Recherche "Bioressourses: Biology Integrative & Valorisation" High Institute of Biotechnology of Monastir, University of Monastir, 5000, Monastir, Tunisia.

This study aimed to identify the physicochemical and the chemical properties of Ailanthus altissima (Miller) Swingle seed oil and to evaluate its in vitro antioxidant and antibacterial activities and in vivo analgesic and anti-inflammatory activities. The fatty acids' composition was determined using GC-FID. The oil was screened for antioxidant activity by DPPH test. Read More

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http://dx.doi.org/10.1007/s11356-019-04659-4DOI Listing
March 2019
1 Read
2.828 Impact Factor

Complete genome sequence of the halophilic PHA-producing bacterium Halomonas sp. SF2003: insights into its biotechnological potential.

World J Microbiol Biotechnol 2019 Mar 9;35(3):50. Epub 2019 Mar 9.

Institut de Recherche Dupuy de Lôme (IRDL), UMR CNRS 6027, Université de Bretagne Sud, Rue Saint Maudé, Lorient, France.

A halophilic Gram-negative eubacterium was isolated from the Iroise Sea and identified as an efficient producer of polyhydroxyalkanoates (PHA). The strain, designated SF2003, was found to belong to the Halomonas genus on the basis of 16S rRNA gene sequence similarity. Previous biochemical tests indicated that the Halomonas sp. Read More

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http://dx.doi.org/10.1007/s11274-019-2627-8DOI Listing
March 2019
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Genetic diversity and population structure of Saccharomyces cerevisiae strains isolated from traditional alcoholic beverages of Côte d'Ivoire.

Int J Food Microbiol 2019 May 3;297:1-10. Epub 2019 Mar 3.

Micalis Institute, INRA, AgroParisTech, CIRM-Levures, Université Paris-Saclay, 78350 Jouy-en-Josas, France.

In order to assess the genetic diversity and population structure of indigenous S. cerevisiae from Côte d'Ivoire, a total of 170 strains were isolated from four traditional alcoholic beverages through nine regions. Microsatellite analysis performed at 12 loci revealed that strains of palm oil and raffia wine were genetically related, unlike those of tchapalo and ron wine which formed two s from palm oil wine and raffia wine were clearly inbred. Read More

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http://dx.doi.org/10.1016/j.ijfoodmicro.2019.03.001DOI Listing
May 2019
2 Reads

Selective auxin agonists induce specific AUX/IAA protein degradation to modulate plant development.

Proc Natl Acad Sci U S A 2019 Mar 8;116(13):6463-6472. Epub 2019 Mar 8.

Department of Forest Genetics and Plant Physiology, Umeå Plant Science Centre, Swedish University of Agricultural Sciences, SE-901 83 Umeå, Sweden;

Auxin phytohormones control most aspects of plant development through a complex and interconnected signaling network. In the presence of auxin, AUXIN/INDOLE-3-ACETIC ACID (AUX/IAA) transcriptional repressors are targeted for degradation by the SKP1-CULLIN1-F-BOX (SCF) ubiquitin-protein ligases containing TRANSPORT INHIBITOR RESISTANT 1/AUXIN SIGNALING F-BOX (TIR1/AFB). CULLIN1-neddylation is required for SCF functionality, as exemplified by mutants deficient in the NEDD8-activating enzyme subunit AUXIN-RESISTANT 1 (AXR1). Read More

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http://www.pnas.org/lookup/doi/10.1073/pnas.1809037116
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http://dx.doi.org/10.1073/pnas.1809037116DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6442611PMC
March 2019
9 Reads
9.809 Impact Factor

Transient plant production of Typhimurium diagnostic antibodies.

Biotechnol Rep (Amst) 2019 Mar 12;21:e00314. Epub 2019 Feb 12.

Technische Universität Braunschweig, Institut für Biochemie, Biotechnologie und Bioinformatik, Abteilung Biotechnologie, Spielmannstr. 7, 38106, Braunschweig, Germany.

Typhimurium is one of the most important zoonotic pathogens worldwide and a major cause of economic losses in the pig production chain. The emergence of multi-drug resistant strains over the past years has led to considerations about an enhanced surveillance of bacterial food contamination. Currently, ELISA is the method of choice for high throughput identification of Typhimurium. Read More

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http://dx.doi.org/10.1016/j.btre.2019.e00314DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6389800PMC
March 2019
1 Read

Phenolic constituents and antioxidant activity of Euphorbia retusa Forssk.

Nat Prod Res 2019 Mar 5:1-3. Epub 2019 Mar 5.

d Laboratoire des Plantes aromatiques et médicinales , Centre de Biotechnologie de Borj-Cédria , Hammam-lif , Tunisia.

The aim of the present study was to evaluate the phenolic contents in capsule and root methanolic extracts of Euphorbia retusa followed by identification of phenolic on capsule extracts through high-performance liquid chromatography (HPLC- DAD) for the first time. Likewise, the antioxidant activity of both extracts was investigated by using Cupric reducing antioxidant capacity (CUPRAC). The result implies that E. Read More

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https://www.tandfonline.com/doi/full/10.1080/14786419.2019.1
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http://dx.doi.org/10.1080/14786419.2019.1582040DOI Listing
March 2019
9 Reads

Characterization, Diversity, and Structure-Activity Relationship Study of Lipoamino Acids from sp. and Synthetic Analogues.

Int J Mol Sci 2019 Mar 2;20(5). Epub 2019 Mar 2.

CNRS, Institut de Chimie des Substances Naturelles, UPR 2301, Université Paris-Saclay, 1 avenue de la Terrasse, 91198 Gif-sur-Yvette, France.

A biological evaluation of a library of extracts from entomopathogen strains showed that sp. extract has significant antimicrobial and insecticidal activities. Three hydroxyacyl-phenylalanine derivatives were isolated from this strain. Read More

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http://dx.doi.org/10.3390/ijms20051083DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6429151PMC
March 2019
3 Reads

Corrigendum to "Han's model parameters for microalgae grown under intermittent illumination: Determined using particle swarm optimization" [Journal of Theoretical Biology 437 (2018) 29-35].

J Theor Biol 2019 May 6;469:201. Epub 2019 Mar 6.

LGPM, CentraleSupélec, Université Paris-Saclay, SFR Condorcet FR CNRS 3417, Centre Européen de Biotechnologie et de Bioéconomie (CEBB), 3 rue des Rouges Terres 51110 Pomacle, France.

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http://dx.doi.org/10.1016/j.jtbi.2019.02.021DOI Listing
May 2019
1 Read