9 results match your criteria Biotechnic & histochemistry : official publication of the Biological Stain Commission[Journal]

  • Page 1 of 1

Effect of addition of buserelin acetate to the extender on motility and viability of bovine spermatozoa.

Anim Biotechnol 2018 Dec 31:1-6. Epub 2018 Dec 31.

b Malopolska Biotechnic Center Ltd , Krasne , Poland.

The present study was aimed to determine the effect of GnRH analog (buserelin acetate) on the quality of bovine spermatozoa stored at 16° C for 24 h. Semen collected in the summer season from June to September from healthy Polish Holstein-Friesian bulls. Ejaculates were centrifuged, divided and diluted to the final concentration of 240 × 10 spermatozoa/mL using animal protein-free commercial BIOXcell extender (IMV Technologies, L'aigle, France) (Control) or with BIOXcell extender supplemented with buserelin acetate and stored 0, 8 and 24 h. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1080/10495398.2018.1521821DOI Listing
December 2018

Use of Mesothelial Cells and Biological Matrices for Tissue Engineering of Simple Epithelium Surrogates.

Front Bioeng Biotechnol 2015 17;3:117. Epub 2015 Aug 17.

Andalusian Center for Molecular Biology and Regenerative Medicine - Centro Andaluz de Biología Molecular y Medicina Regenerativa (CABIMER) , Seville , Spain ; Centro de Investigación en Red sobre Diabetes y Enfermedades Metabólicas (CIBERDEM) , Madrid , Spain.

Tissue-engineering technologies have progressed rapidly through last decades resulting in the manufacture of quite complex bioartificial tissues with potential use for human organ and tissue regeneration. The manufacture of avascular monolayered tissues such as simple squamous epithelia was initiated a few decades ago and is attracting increasing interest. Their relative morphostructural simplicity makes of their biomimetization a goal, which is currently accessible. Read More

View Article

Download full-text PDF

Source
http://journal.frontiersin.org/Article/10.3389/fbioe.2015.00
Publisher Site
http://dx.doi.org/10.3389/fbioe.2015.00117DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4538307PMC
September 2015
4 Reads

Mesothelial cells: a cellular surrogate for tissue engineering of corneal endothelium.

Invest Ophthalmol Vis Sci 2014 Aug 19;55(9):5967-78. Epub 2014 Aug 19.

Andalusian Center for Molecular Biology and Regenerative Medicine (CABIMER), Department of Stem Cells, Sevilla, Spain Centro de Investigación Biomédica en Red-Diabetes y Enfermedades Metabólicas (CIBERDEM), Instituto de Salud Carlos III, Madrid, Spain.

Purpose: To evaluate whether mouse adipose tissue mesothelial cells (ATMCs) share morphologic and biochemical characteristics with mouse corneal endothelial cells (CECs) and to evaluate their capacity to adhere to the decellularized basal membrane of human anterior lens capsules (HALCs) as a potential tissue-engineered surrogate for corneal endothelium replacement.

Methods: Adipose tissue mesothelial cells were isolated from the visceral adipose tissue of adult mice, and their expression of several corneal endothelium markers was determined with quantitative RT-PCR, immunofluorescence, and Western blotting. Adipose tissue mesothelial cells were cultured in a mesothelial retaining phenotype medium (MRPM) and further seeded and cultured on top of the decellularized basal membrane of HALCs. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1167/iovs.14-14706DOI Listing
August 2014
2 Reads

Accomplishments of the Trustees and laboratory staff of the Biological Stain Commission, 2002-2013.

Authors:
R W Dapson

Biotech Histochem 2014 Aug 25;89(6):470-9. Epub 2014 Mar 25.

Dapson & Dapson, LLC , 6951 East AB Avenue, Richland, Michigan 49083.

During the 12 years from 2002 to 2013, the Trustees and laboratory personnel of the Biological Stain Commission (BSC) can claim many accomplishments. These accomplishments are itemized under 11 categories: continuous publication of the official journal, Biotechnic & Histochemistry; production of four special issues of Biotechnic & Histochemistry devoted to specific dyes or stains; standardization of staining and dye purity; mechanisms of staining and prediction of dye behavior; publication of books or book chapters; effects of fixation and processing on staining; cancer research; immunohistochemistry; BSC Laboratory activities; miscellaneous publications; and administrative accomplishments. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3109/10520295.2014.892634DOI Listing

[Detection of Chlamydia in inflammation of the cervix and uterus in cows].

Vet Med (Praha) 1996 Oct;41(10):297-304

Veterinary Research Institute, Brno, Czech Republic.

Clinical findings in cows of seven farms with a frequent occurrence of birth canal inflammations were matched against results of serological and direct immunofluorescence tests for Chlamydia sp. infection. Normal clinical picture, weak to moderate and serious inflammations were found in 16. Read More

View Article

Download full-text PDF

Source
October 1996

[Detection of chlamydiae in animal and human semen using direct immunofluorescence].

Vet Med (Praha) 1996 Jul;41(7):201-6

Veterinary Research Institute, Brno, Czech Republic.

Frequency of elementary and reticular chlamydial bodies was investigated by direct immunofluorescence tests in ejaculates collected from 52 men, 60 stallions, 42 bulls, and 66 boars using the kits of Progen Biotechnic GmbH and the microscope Labophot-2 Nikon. At the same time, qualitative semen tests, including ejaculate volume, sperm motility, percentage of live and dead sperms and morphological' analyses (Vĕzník and Svecová, 1992) were done. Repeatability of the findings was checked in a group of nine bulls housed at the institute and sampled at weekly intervals for 3 to 4 months (Tab. Read More

View Article

Download full-text PDF

Source

Fluorescent labelling of sequencing primers for automated oligonucleotide synthesis.

DNA Seq 1992 ;2(5):273-9

FZB Biotechnic GmbH, Berlin.

A fluorescein derivative is described which can be used as a normal phosphoramidite in oligonucleotide synthesis, giving high yields of fluorescein labelled sequencing primers. The labelled primers were used in automated DNA-sequence analysis without modification of existing protocols, the computer-processed sequences being reproducibly readable up to 400 bases. The procedure described makes the fluorescent labelling of oligonucleotides much easier, and the time of labelling can be significantly reduced. Read More

View Article

Download full-text PDF

Source

[Total prosthesis. The role of biotechnic in stomatological prosthesis].

Authors:
G Tanzer

Zahntechnik (Berl) 1975 Nov;16(11):471-6

View Article

Download full-text PDF

Source
November 1975

[Biotechnic in the Formation Professionnelle].

Authors:
R BUFFEL

Arch Mal Prof 1953 ;14(3):265-7

View Article

Download full-text PDF

Source
  • Page 1 of 1