63,607 results match your criteria Biosensors & Bioelectronics [Journal]


Genetically encoded biosensors for the detection of rapamycin: toward the screening of agonists and antagonists.

Analyst 2020 Jul 3. Epub 2020 Jul 3.

Department of Biomedical Engineering (BK21 plus), Dongguk University, Seoul 04620, Korea.

Biosensors are valuable tools for the rapid screening of biological targets with high sensitivity and specificity. It is important to screen biological events in their native context for pharmacological and toxicological applications. However, in vitro biosensors often require purified probes and targets for screening, thus providing limited information on the biological activities of targets in their native environment. Read More

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http://dx.doi.org/10.1039/d0an01116aDOI Listing

A Label-Free and Regenerable Aptasensor for Real-Time Detection of Cadmium (II) by Dual Polarization Interferometry.

Anal Chem 2020 Jul 3. Epub 2020 Jul 3.

Recently, numerous aptamer-based biosensors have been developed to detect heavy metal ions. Most of aptamer-based biosensors only can be used to get some quantitative information. The mechanism of the interaction between aptamer and metal ions, however, is rarely studied. Read More

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http://dx.doi.org/10.1021/acs.analchem.0c01710DOI Listing

Ultrasonic atomizer based development of pH sensor for real time analysis.

Sci Rep 2020 Jul 2;10(1):10910. Epub 2020 Jul 2.

Room No. SB-216, Discipline of Biosciences and Biomedical Engineering, Indian Institute of Technology Indore, Khandwa Road, Simrol, Indore, Madhya Pradesh, 453552, India.

Fluorescent pH biosensors have gained importance owing to their low cost utilization in real time monitoring of biological and food samples in comparison to conventional pH meters. The research reports a novel method of ultrasonic atomization for developing a fluorescent pH sensor for real-time analysis made of Fluorescein isothiocyanate (FITC)-dextran/FITC-dextran-Tris (2, 2'-bipyridyl) dichlororuthenium (II) hexahydrate as indicator and reference fluorophores, respectively. The process of ultrasonic atomization ensures formation of monodisperse dye immobilized alginate microspheres ensuring efficient pH sensing. Read More

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http://dx.doi.org/10.1038/s41598-020-68005-2DOI Listing

Dual plasmonic nanostructures for switching polarity of hot electron-induced photocurrent.

Nanoscale 2020 Jul 2. Epub 2020 Jul 2.

School of Physics and National Laboratory of Solid State Microstructures, Collaborative Innovation Center of Advanced Microstructures, Nanjing University, Nanjing 210093, China.

We report on the experimental investigation of polarity-switchable hot electron-induced photocurrents in dual-plasmonic nanostructures, consisting of two layers of gold nanoparticles (AuNPs) separated by a TiO2 film. Hot electrons generated through the non-radiative decay of the localized surface plasmon resonances supported by the top and bottom layers of AuNPs can be simultaneously injected into the TiO2 film in opposite directions and counteract each other. As a result, the polarity and magnitude of the net photocurrents can be tailored by controlling the population of hot electrons either generated from or collected by the two layers of AuNPs. Read More

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http://dx.doi.org/10.1039/c9nr10413eDOI Listing

Enzyme-based optical biosensors for organophosphate class of pesticide detection.

Phys Chem Chem Phys 2020 Jul 2. Epub 2020 Jul 2.

Radiation & Photochemistry Division, Bhabha Atomic Research Centre, Mumbai 400 085, India. and Homi Bhabha National Institute, Training School Campus, Anushaktinagar, Mumbai 400 094, India.

Organophosphorus pesticides, widely used as insecticides for crop protection, are classified as an extremely toxic class of chemical compounds by the World Health Organization (WHO). Organophosphorus pesticides show marked specificity for the enzyme acetylcholinesterase, and can cause irreversible harm to the nervous system. The excessive use of organophosphorus pesticides, specifically in developing countries, made WHO to impose a ban on certain organophosphorus pesticides being sold to the developing nations. Read More

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http://dx.doi.org/10.1039/d0cp01647kDOI Listing

A hybrid system integrating xylose dehydrogenase and NAD coupled with PtNPs@MWCNTs composite for the real-time biosensing of xylose.

Analyst 2020 Jul 2. Epub 2020 Jul 2.

Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 32 West Seventh Avenue, Tianjin Airport Economic Area, Tianjin, 300308, P. R. China. and School of Chemical Engineering, University of Chinese Academy of Sciences, 19A Yuquan Road, Shijingshan District, Beijing, 100049, P. R. China.

The wide application of xylose in the food, beverage, and pharmaceutical industries, as well as in the booming field of biorefinery, raises the demand for a rapid, accurate, and real-time xylose-sensing technique to rival the conventional methods based on chromatography, spectroscopy, and electrochemical analysis using non-specific enzymes or abiotic catalysts. Herein, a hybrid system comprising polyethylene glycerol swing-arm-tethered NAD+ and xylose dehydrogenase (XDH), coupled with platinum nanoparticles deposited on carbon nanotubes (PtNPs@MWCNTs), was constructed for the real-time sensing of xylose. The use of the PtNPs@MWCNTs composite enhanced the sensitivity of the electric response and reduced the oxidation potential of NADH significantly. Read More

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http://dx.doi.org/10.1039/d0an00880jDOI Listing

A Flexible 3D Organic Preamplifier for a Lactate Sensor.

Macromol Biosci 2020 Jul 1:e2000144. Epub 2020 Jul 1.

Department of Creative IT Engineering / Department of Chemical Engineering, Pohang University of Science and Technology (POSTECH), 77 Cheongam-Ro, Nam-Gu, Pohang, 37673, Republic of Korea.

Organic transistors are promising platforms for wearable biosensors. However, the strategies to improve signal amplification have yet to be determined, particularly regarding biosensors that generate very weak signals. In this study, an organic voltage amplifier is presented for a lactate sensor on flexible plastic foil. Read More

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http://dx.doi.org/10.1002/mabi.202000144DOI Listing

Simple experimental procedures to distinguish photothermal from hot-carrier processes in plasmonics.

Light Sci Appl 2020 28;9:108. Epub 2020 Jun 28.

ICFO-Institut de Ciències Fotòniques, The Barcelona Institute of Science and Technology, 08860 Castelldefels, Barcelona, Spain.

Light absorption and scattering of plasmonic metal nanoparticles can lead to non-equilibrium charge carriers, intense electromagnetic near-fields, and heat generation, with promising applications in a vast range of fields, from chemical and physical sensing to nanomedicine and photocatalysis for the sustainable production of fuels and chemicals. Disentangling the relative contribution of thermal and non-thermal contributions in plasmon-driven processes is, however, difficult. Nanoscale temperature measurements are technically challenging, and macroscale experiments are often characterized by collective heating effects, which tend to make the actual temperature increase unpredictable. Read More

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http://dx.doi.org/10.1038/s41377-020-00345-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7321931PMC

Application of Nanostructured Carbon-Based Electrochemical (Bio)Sensors for Screening of Emerging Pharmaceutical Pollutants in Waters and Aquatic Species: A Review.

Nanomaterials (Basel) 2020 Jun 29;10(7). Epub 2020 Jun 29.

REQUIMTE-LAQV, Instituto Superior de Engenharia do Porto, Instituto Politécnico do Porto, Rua Dr. António Bernardino de Almeida, 431, 4249-015 Porto, Portugal.

Pharmaceuticals, as a contaminant of emergent concern, are being released uncontrollably into the environment potentially causing hazardous effects to aquatic ecosystems and consequently to human health. In the absence of well-established monitoring programs, one can only imagine the full extent of this problem and so there is an urgent need for the development of extremely sensitive, portable, and low-cost devices to perform analysis. Carbon-based nanomaterials are the most used nanostructures in (bio)sensors construction attributed to their facile and well-characterized production methods, commercial availability, reduced cost, high chemical stability, and low toxicity. Read More

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http://dx.doi.org/10.3390/nano10071268DOI Listing

Challenges for Microelectronics in Non-Invasive Medical Diagnostics.

Sensors (Basel) 2020 Jun 29;20(13). Epub 2020 Jun 29.

Politecnico di Milano, Dipartimento di Elettronica Informazione e Bioingegneria, 20133 Milano, Italy.

Microelectronics is emerging, sometimes with changing fortunes, as a key enabling technology in diagnostics. This paper reviews some recent results and technical challenges which still need to be addressed in terms of the design of CMOS analog application specific integrated circuits (ASICs) and their integration in the surrounding systems, in order to consolidate this technological paradigm. Open issues are discussed from two, apparently distant but complementary, points of view: micro-analytical devices, combining microfluidics with affinity bio-sensing, and gamma cameras for simultaneous multi-modal imaging, namely scintigraphy and magnetic resonance imaging (MRI). Read More

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http://dx.doi.org/10.3390/s20133636DOI Listing

Technologies of time: women's practices of trying to conceive with ovulation biosensing.

Authors:
Joann Wilkinson

Sociol Health Illn 2020 Jul 1. Epub 2020 Jul 1.

Department of Educational Research, Lancaster University, Lancaster, UK.

Ovulation biosensors are devices worn on or used with the body, which can help women detect ovulation. The manufacturers of such devices claim that if women know when they ovulate, couples can arrange heterosexual intercourse during this time, and thus increase their chances of conceiving. Within the contemporary UK context, in which becoming pregnant is presented in the popular media, and in medical discourses, as more difficult for women in their thirties and forties, manufacturers' claims are attractive for those trying to conceive. Read More

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http://dx.doi.org/10.1111/1467-9566.13150DOI Listing

High-Sensitive Assay of Nucleic Acid Using Tetrahedral DNA Probes and DNA Concatamers with a Surface-Enhanced Raman Scattering/Surface Plasmon Resonance Dual-Mode Biosensor Based on a Silver Nanorod-Covered Silver Nanohole Array.

ACS Appl Mater Interfaces 2020 Jul 1. Epub 2020 Jul 1.

Key Laboratory for Organic Electronics and Information Displays & Jiangsu Key Laboratory for Biosensors, Institute of Advanced Materials (IAM), Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM), Nanjing University of Posts & Telecommunications, Nanjing 210023, China.

A novel surface-enhanced Raman scattering/surface plasmon resonance (SERS/SPR) dual-mode biosensor prepared on a silver nanorod-covered silver nanohole (Ag NR-NH) array by surface modification of tetrahedral DNA probes is proposed for highly sensitive detecting nucleic acids by a special signal amplification strategy of DNA supersandwich. The Ag NR-NH with a large area and uniformly arrayed nanostructure possesses excellent anisotropic extraordinary optical transmission and strong localized surface plasmon resonance, which lead to sensitive SPR response to the change of a local refractive index and strong localized electric fields for excellent SERS activity. To obtain high sensitivity and specificity, smart tetrahedral DNA probes are immobilized onto the Ag NR-NH array and the DNA supersandwich sensing strategy, including the signal amplification of DNA concatamers, is used. Read More

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http://dx.doi.org/10.1021/acsami.0c08453DOI Listing

Extending the Debye scattering equation for diffraction from a cylindrically averaged group of atoms: detecting molecular orientation at an interface.

Acta Crystallogr A Found Adv 2020 Jul 20;76(Pt 4):468-473. Epub 2020 May 20.

School of Physics A28, The University of Sydney, NSW 2006, Australia.

The Debye scattering equation is now over 100 years old and has been widely used to interpret diffraction patterns from randomly oriented groups of atoms. The present work develops and applies a related equation that calculates diffraction intensity from groups of atoms randomly oriented about a fixed axis, a scenario that occurs when molecules are oriented at an interface by the presentation of a binding motif as in antibody binding. Using an example biomolecule, the high level of sensitivity of the diffraction pattern to the orientation of the molecule and to the direction of the incident beam is shown. Read More

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http://dx.doi.org/10.1107/S2053273320005276DOI Listing

Efficient synthesis of amino acids capped gold nanoparticles from easily reducible aryldiazonium tetrachloroaurate(III) salts for cellular uptake study.

Amino Acids 2020 Jun 30. Epub 2020 Jun 30.

Center for Advanced Materials Research, Research Institute for Science and Engineering, University of Sharjah, 27272, Sharjah, United Arab Emirates.

Biomimetic synthesis of gold nanoparticles (GNPs) is critical in biomedical applications. Gold bioconjugates were fabricated by capping the water-dispersible gold-carbon nanoparticles with tyrosine, tryptophan and cysteine amino acids. Incubation of the water-soluble and easily reducible aryldiazonium gold(III) salt [HOOC-4-CHN≡N]AuCl with amino acids at room temperature formed a purple color over a few minutes with tryptophan and tyrosine and over two hours with cysteine. Read More

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http://dx.doi.org/10.1007/s00726-020-02862-zDOI Listing

Biofunctional Polymer Coated Au Nanoparticles Prepared via RAFT-Assisted Encapsulating Emulsion Polymerization and Click Chemistry.

Polymers (Basel) 2020 Jun 27;12(7). Epub 2020 Jun 27.

Department of Chemistry, CICECO-Aveiro Institute of Materials, University of Aveiro, 3810-193 Aveiro, Portugal.

The use of reversible addition-fragmentation chain transfer (RAFT)-assisted encapsulating emulsion polymerization (REEP) has been explored to prepare diverse types of colloidal stable core-shell nanostructures. A major field of application of such nanoparticles is in emergent nanomedicines, which require effective biofunctionalization strategies, in which their response to bioanalytes needs to be firstly assessed. Herein, functional core-shell nanostructures were prepared via REEP and click chemistry. Read More

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http://dx.doi.org/10.3390/polym12071442DOI Listing

Biosensors for D-Amino Acids: Detection Methods and Applications.

Int J Mol Sci 2020 Jun 27;21(13). Epub 2020 Jun 27.

Department of Biotechnology and Life Sciences, University of Insubria, via J.H. Dunant 3, 21100 Varese, Italy.

D-enantiomers of amino acids (D-AAs) are only present in low amounts in nature, frequently at trace levels, and for this reason, their biological function was undervalued for a long time. In the past 25 years, the improvements in analytical methods, such as gas chromatography, HPLC, and capillary electrophoresis, allowed to detect D-AAs in foodstuffs and biological samples and to attribute them specific biological functions in mammals. These methods are time-consuming, expensive, and not suitable for online application; however, life science investigations and industrial applications require rapid and selective determination of D-AAs, as only biosensors can offer. Read More

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http://dx.doi.org/10.3390/ijms21134574DOI Listing
June 2020
2.862 Impact Factor

Biofabrication offers future hope for tackling various obstacles and challenges in tissue engineering and regenerative medicine: A Perspective.

Int J Bioprint 2019 31;5(1):153. Epub 2018 Dec 31.

Graduate School of Science and Engineering for Research (Engineering), University of Toyama, Toyama 930-8555, Japan.

Biofabrication is an emerging multidisciplinary field that makes a revolutionary impact on the researches on life science, biomedical engineering, and both basic and clinical medicine, has progressed tremendously over the past few years. Recently, there has been a big boom in three-dimensional (3D) printing or additive manufacturing (AM) research worldwide, and there is a significant increase not only in the number of researchers turning their attention to AM but also publications demonstrating the potential applications of 3D printing techniques in multiple fields. Biofabrication and bioprinting hold great promise for the innovation of engineering-based organ replacing medicine. Read More

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http://dx.doi.org/10.18063/ijb.v5i1.153DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7294687PMC
December 2018

Biosensors for detection of Tau protein as an Alzheimer's disease marker.

Int J Biol Macromol 2020 Jun 27. Epub 2020 Jun 27.

Research Center for Biochemistry and Nutrition in Metabolic Diseases, Institute for Basic Sciences, Kashan University of Medical Sciences, Kashan, Iran. Electronic address:

Known as a main neural MAP (microtubule associated protein), tau protein contributes to stabilizing microtubules involved in cellular transmission. Tau dysfunction is mainly associated with neurodegenerative diseases, particularly Alzheimer's disease (AD). In these patients, all the six tau isoforms, which are in hyperphosphorylated form, are first aggregated and then polymerized into neurofibrillary tangles inside the brain. Read More

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http://dx.doi.org/10.1016/j.ijbiomac.2020.06.239DOI Listing

New Approach for Making Standard the Development of Biosensing Devices by a Modular Multi-Purpose Design.

IEEE Trans Nanobioscience 2020 Jul;19(3):339-346

The fast widening of biosensing applications, such as healthcare, drug delivery, food, and military industries, is increasing the need for generality and compatibility among different sensors. To address this challenge, we present here an innovative approach for the fast development of new electronic biosensing systems, linking a custom-designed front-end with a multi-purpose system. We envision an open tool to help designers to focus on the target molecule and related detection method instead of designing each time a dedicated electronic device. Read More

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http://dx.doi.org/10.1109/TNB.2020.2995230DOI Listing

Functional nucleic acid-based fluorescence polarization/anisotropy biosensors for detection of biomarkers.

Anal Bioanal Chem 2020 Jun 29. Epub 2020 Jun 29.

Research Center for Bioengineering and Sensing Technology, School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing, 100083, China.

The sensitive and selective detection of biomarkers plays a crucial role in disease diagnostics, drug discovery, and early screening of cancers. The achievement of this goal highly depends on the continuous development of biosensing technologies. Among them, fluorescence anisotropy/polarization (FA/FP) analysis receives increasing interest due to the advantage of simple operation, fast response, and no background interference. Read More

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http://dx.doi.org/10.1007/s00216-020-02754-xDOI Listing

Real-time monitoring of peroxiredoxin oligomerization dynamics in living cells.

Proc Natl Acad Sci U S A 2020 Jun 29. Epub 2020 Jun 29.

Division of Redox Regulation, DKFZ-ZMBH Alliance, German Cancer Research Center (DKFZ), 69120 Heidelberg, Germany;

Peroxiredoxins are central to cellular redox homeostasis and signaling. They serve as peroxide scavengers, sensors, signal transducers, and chaperones, depending on conditions and context. Typical 2-Cys peroxiredoxins are known to switch between different oligomeric states, depending on redox state, pH, posttranslational modifications, and other factors. Read More

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http://dx.doi.org/10.1073/pnas.1915275117DOI Listing

Immunosensors containing solution blow spun fibers of poly(lactic acid) to detect p53 biomarker.

Mater Sci Eng C Mater Biol Appl 2020 Oct 27;115:111120. Epub 2020 May 27.

São Carlos Institute of Physics, University of São Paulo, 13566-590 São Carlos, Brazil. Electronic address:

This paper reports on biosensors made with a matrix of polylactic acid (PLA) fibers, which are suitable for immobilization of the anti-p53 active layer for detection of p53 biomarker. The PLA fibers were produced with solution blow spinning, a method that is advantageous for its simplicity and possibility to tune the fiber properties. For the biosensors, the optimized time to deposit the fibers was 60 s, with which detection of p53 could be achieved with the limit of detection of 11 pg/mL using electrical impedance spectroscopy. Read More

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http://dx.doi.org/10.1016/j.msec.2020.111120DOI Listing
October 2020

Osteoblastic exosomes. A non-destructive quantitative approach of alkaline phosphatase to assess osteoconductive nanomaterials.

Mater Sci Eng C Mater Biol Appl 2020 Oct 13;115:110931. Epub 2020 Apr 13.

Grup de Sensors i Biosensors, Departament de Química, Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain.

Alkaline phosphatase (ALP) is an essential biomarker of osteoblastic activity. Currently, ALP activity has been used to study bone mineralization mechanisms and osteoactive biomaterials among others. The ALP quantification is usually performed by destructive methods either on growing cells or cells lysate in which the osteoconductive biomaterial is being assessed. Read More

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http://dx.doi.org/10.1016/j.msec.2020.110931DOI Listing
October 2020

Interplay of Effective Surface Area, Mass Transport, and Electrochemical Features in Nanoporous Nucleic Acid Sensors.

Anal Chem 2020 Jun 30. Epub 2020 Jun 30.

Electrochemical biosensors transduce biochemical events (e.g., DNA hybridization) to electrical signals and can be readily inter-faced with electronic instrumentation for portability. Read More

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http://dx.doi.org/10.1021/acs.analchem.0c02104DOI Listing

Detection of Cardiovascular CRP Protein Biomarker Using a Novel Nanofibrous Substrate.

Biosensors (Basel) 2020 Jun 24;10(6). Epub 2020 Jun 24.

Department of Biomedical Engineering, University of Bridgeport, Bridgeport, CT 06604, USA.

It is known that different diseases have characteristic biomarkers that are secreted very early on, even before the symptoms have developed. Before any kind of therapeutic approach can be used, it is necessary that such biomarkers be detected at a minimum concentration in the bodily fluids. Here, we report the fabrication of an interdigitated sensing device integrated with polyvinyl alcohol (PVA) nanofibers and carbon nanotubes (CNT) for the detection of an inflammatory biomarker, C-reactive protein (CRP). Read More

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http://dx.doi.org/10.3390/bios10060072DOI Listing

A Capillary-Perfused, Nanocalorimeter Platform for Thermometric Enzyme-Linked Immunosorbent Assay with Femtomole Sensitivity.

Biosensors (Basel) 2020 Jun 24;10(6). Epub 2020 Jun 24.

Department of Biomedical Engineering, Vanderbilt University, Nashville, TN 37235, USA.

Enzyme-catalyzed chemical reactions produce heat. We developed an enclosed, capillary-perfused nanocalorimeter platform for thermometric enzyme-linked immunosorbent assay (TELISA). We used catalase as enzymes to model the thermal characteristics of the micromachined calorimeter. Read More

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http://dx.doi.org/10.3390/bios10060071DOI Listing

Activity- and pH-dependent adenosine shifts at the end of a focal seizure in the entorhinal cortex.

Epilepsy Res 2020 Jun 15;165:106401. Epub 2020 Jun 15.

Epilepsy Unit, Fondazione IRCCS Istituto Neurologico Carlo Besta, via Amadeo 42, 20133 Milano, Italy. Electronic address:

Adenosine (ADO) is an endogenous modulator of neuronal excitability, with anticonvulsant and neuroprotective effects. It has been proposed that the activity-dependent release of ADO promoted by the extracellular acidification occurring during seizures contributes to seizure termination. To verify this hypothesis, we recorded field potentials, pH and ADO changes measured with enzymatic biosensors during acute focal seizures in the medial entorhinal cortex (mEC) of the isolated guinea-pig brain maintained in vitro. Read More

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http://dx.doi.org/10.1016/j.eplepsyres.2020.106401DOI Listing

Metal-Organic Framework Derived Nanozymes in Biomedicine.

Acc Chem Res 2020 Jun 29. Epub 2020 Jun 29.

Division of Chemistry and Biological Chemistry, School of Physical and Mathematical Sciences, Nanyang Technological University, 21 Nanyang Link, Singapore 637371, Singapore.

ConspectusNanozymes, which integrate the advantages of both nanomaterials and natural enzymes, have accumulated enormous research interest over the past decades because of the opportunity they provide to appreciate and further cultivate artificial enzymes with comparable properties. By mimicking the coordination environments of the catalytic sites in natural enzymes, nanozymes with confined nanostructures can serve as substitutes in many catalytic processes with comparable activity and robust stability even in harsh conditions. Since the pioneering report about peroxidase-mimicking ferromagnetic nanoparticles in 2007, nanozymes have been developed as specialized for nanomaterials with intrinsic enzyme-mimicking property. Read More

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http://dx.doi.org/10.1021/acs.accounts.0c00268DOI Listing

Self-immolative polymers in biomedicine.

J Mater Chem B 2020 Jun 29. Epub 2020 Jun 29.

Department of Chemistry and Chemical Biology, Northeastern University, Boston, Massachusetts 02115, USA.

Self-immolative polymers (SIPs) have been under development for over a decade, and efforts for their application followed shortly after their inception. One main area of application of SIPs is biomedicine, where they are used to construct devices and biosensors, develop new biotechnology abilities, or directly interface with the living system. Where traditional polymers are stable at room temperature, SIPs undergo rapid degradation when a labile capping group is removed, allowing SIPs to offer a highly unusual degradation profile compared with traditional polymers. Read More

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http://dx.doi.org/10.1039/d0tb01119cDOI Listing

[Progress in fluorescent visualization techniques for neurotransmitter detection].

Sheng Wu Gong Cheng Xue Bao 2020 Jun;36(6):1051-1059

Chongqing Key Laboratory of Nano/Micro Composite Materials and Devices, Chongqing University of Science and Technology, Chongqing 401331, China.

Neurotransmitters play an important role in nervous system. Temporal and spatial changes of neurotransmitter distribution are crucial to information processing in neural networks. Biosensors that can visually monitor neurotransmitters are one of the vital tools to explore a variety of physiological and pathological activities. Read More

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http://dx.doi.org/10.13345/j.cjb.190441DOI Listing

Single Iron Site Nanozyme for Ultrasensitive Glucose Detection.

Small 2020 Jun 29:e2002343. Epub 2020 Jun 29.

Department of Chemistry, Hefei National Laboratory for Physical Sciences at the Microscale, iChEM (Collaborative Innovation Center of Chemistry for Energy Materials), University of Science and Technology of China, Hefei, 230026, China.

Nanomaterials with enzyme-mimicking characteristics have engaged great awareness in various fields owing to their comparative low cost, high stability, and large-scale preparation. However, the wide application of nanozymes is seriously restricted by the relatively low catalytic activity and poor specificity, primarily because of the inhomogeneous catalytic sites and unclear catalytic mechanisms. Herein, a support-sacrificed strategy is demonstrated to prepare a single iron site nanozyme (Fe SSN) dispersed on the porous N-doped carbon. Read More

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http://dx.doi.org/10.1002/smll.202002343DOI Listing

DNA-Based Plasmonic Heterogeneous Nanostructures: Building, Optical Responses, and Bioapplications.

Adv Mater 2020 Jun 29:e1907880. Epub 2020 Jun 29.

International Joint Research Laboratory for Biointerface and Biodetection, State Key Lab of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, 214122, China.

The integration of multiple functional nanoparticles into a specific architecture allows the precise manipulation of light for coherent electron oscillations. Plasmonic metals-based heterogeneous nanostructures are fabricated by using DNA as templates. This comprehensive review provides an overview of the controllable synthesis and self-assembly of heterogeneous nanostructures, and analyzes the effects of structural parameters on the regulation of optical responses. Read More

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http://dx.doi.org/10.1002/adma.201907880DOI Listing

An alternative molecular approach for rapid and specific detection of clinically relevant bacteria causing prosthetic joint infections with bacteriophage K.

New Microbiol 2020 Jun 29;43(3). Epub 2020 Jun 29.

Valdoltra Orthopaedic Hospital, Research department, Ankaran, Slovenia.

Prosthetic joint infections (PJI) represent the most serious cause of prosthetic joint loosening, with high impact on patient life and health economics. Although not entirely reliable, the cultivation of intraoperative prosthetic tissue or synovial fluid remains the gold standard for determining the cause of PJI. Therefore, molecular methods are increasingly being introduced. Read More

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Dual Pathway Inhibition for Vascular Protection in Patients with Atherosclerotic Disease: Rationale and Review of the Evidence.

Thromb Haemost 2020 Jun 28. Epub 2020 Jun 28.

Research and Development Pharmaceuticals, Bayer AG, Wuppertal, Germany.

Despite advances in secondary prevention strategies in patients with cardiovascular disease, the residual risk of recurrent atherothrombotic events remains high. Dual-antiplatelet therapy is the standard of care for secondary prevention in patients with acute coronary syndrome (ACS), whereas single antiplatelet therapy, generally with aspirin, is the standard of care for secondary prevention in stable patients with coronary artery disease (CAD), peripheral artery disease (PAD), or cerebrovascular disease. However, atherosclerotic plaque disruption not only triggers platelet activation but also results in thrombin generation because of tissue factor exposure. Read More

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http://dx.doi.org/10.1055/s-0040-1713376DOI Listing

Differential endothelial signaling responses elicited by chemogenetic HO synthesis.

Redox Biol 2020 Jun 16;36:101605. Epub 2020 Jun 16.

Division of Cardiovascular Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, 75 Francis Street, Boston, MA, 02115, USA. Electronic address:

Hydrogen peroxide (HO) modulates critical phosphorylation pathways in vascular endothelial cells, many of which affect endothelial nitric oxide synthase (eNOS) signal transduction. Both intracellular and extracellular sources of HO have been implicated in eNOS regulation, yet the specific endothelial pathways remain incompletely understood. Here we exploited chemogenetic approaches and live-cell imaging methods to both generate and detect HO in different subcellular compartments (cytosol, nucleus, and caveolae) of cultured EA. Read More

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http://dx.doi.org/10.1016/j.redox.2020.101605DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7322171PMC

Three dimensional printed degradable and conductive polymer scaffolds promote chondrogenic differentiation of chondroprogenitor cells.

Biomater Sci 2020 Jun 26. Epub 2020 Jun 26.

School of Pharmacy, University of Nottingham, Nottingham, NG7 2RD, UK. and Biodiscovery Institute, University of Nottingham, Nottingham, NG7 2RD, UK.

Conductive polymers have been used for various biomedical applications including biosensors, tissue engineering and regenerative medicine. However, the poor processability and brittleness of these polymers hinder the fabrication of three-dimensional structures with desirable geometries. Moreover, their application in tissue engineering and regenerative medicine has been so far limited to excitable cells such as neurons and muscle cells. Read More

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http://dx.doi.org/10.1039/d0bm00621aDOI Listing

A target-mediated fuel-initiated molecular machine for high-sensitive fluorescence assay of the ZIKV gene via strand displacement reaction-based signal recovery and cycling amplification.

Analyst 2020 Jun 26. Epub 2020 Jun 26.

Key Laboratory for Organic Electronics and Information Displays & Jiangsu Key Laboratory for Biosensors, Institute of Advanced Materials (IAM), Jiangsu National Synergistic Innovation Center for Advanced Materials (SICAM), Nanjing University of Posts and Telecommunications, Nanjing, 210023, China.

Zika virus (ZIKV) is a serious threat to human health due to its widespread human arboviral infections, and early diagnosis is one of the keys to preventing infections from spreading, but there is a lack of highly sensitive and specific detection. In this work, a target-mediated fuel-initiated molecular machine was proposed for the high-sensitive fluorescence assay of the ZIKV gene via strand displacement reaction-based signal recovery and cycling amplification. The molecular machine was prepared by modifying AuNP surfaces with specially designed lock-like DNAs (LLDs). Read More

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http://dx.doi.org/10.1039/d0an00854kDOI Listing

Bacterial Sensing and Biofilm Monitoring for Infection Diagnostics.

Macromol Biosci 2020 Jun 26:e2000129. Epub 2020 Jun 26.

AIMES-Center for the Advancement of Integrated Medical and Engineering Science, Karolinska Institutet and KTH Royal Institute of Technology, Stockholm, SE-171 77, Sweden.

Recent insights into the rapidly emerging field of bacterial sensing and biofilm monitoring for infection diagnostics are discussed as well as recent key developments and emerging technologies in the field. Electrochemical sensing of bacteria and bacterial biofilm via synthetic, natural, and engineered recognition, as well as direct redox-sensing approaches via algorithm-based optical sensing, and tailor-made optotracing technology are discussed. These technologies are highlighted to answer the very critical question: "how can fast and accurate bacterial sensing and biofilm monitoring be achieved? Following on from that: "how can these different sensing concepts be translated for use in infection diagnostics? A central obstacle to this transformation is the absence of direct and fast analysis methods that provide high-throughput results and bio-interfaces that can control and regulate the means of communication between biological and electronic systems. Read More

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http://dx.doi.org/10.1002/mabi.202000129DOI Listing

An overview and future prospects on aptamers for food safety.

Appl Microbiol Biotechnol 2020 Jun 25. Epub 2020 Jun 25.

Department of Chemical Engineering and Food Engineering (EQA), Federal University of Santa Catarina (UFSC), Florianópolis, SC, 88040-900, Brazil.

Introduction: Many bacteria are responsible for infections in humans and plants, being found in vegetables, water, and medical devices. Most bacterial detection methods are time-consuming and take days to give the result. Aptamers are a promising alternative for a quick and reliable measurement technique to detect bacteria present in food products. Read More

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http://dx.doi.org/10.1007/s00253-020-10747-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7315907PMC

Periprocedural myocardial injury: Pathophysiology, prognosis, and prevention.

Cardiovasc Revasc Med 2020 May 26. Epub 2020 May 26.

MedStar Washington Hospital Center, Washington, DC, USA. Electronic address:

The definition and clinical implications of myocardial infarction occurring in the setting of percutaneous coronary intervention have been the subject of unresolved controversy. The definitions of periprocedural myocardial infarction (PMI) are many and have evolved over recent years. Additionally, the recent advancement of different imaging modalities has provided useful information on a patients' pre-procedural risk of myocardial infarction. Read More

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http://dx.doi.org/10.1016/j.carrev.2020.04.011DOI Listing

Scattering Fourier Transform Biosensor (SFTB): Binary Mixture Consisting of Magnetic Ni Nanorings and Plasmonic Au Nanorods.

Anal Chem 2020 Jun 26. Epub 2020 Jun 26.

We report a biosensing platform based on a binary mixture comprised of Au nanorods (plasmonic nanoparticles, Au NRs) and magnetically responsive Pt@Ni nanorings (magnetic nanostirrers, MN-rings). The mixture of Au NRs and MN-rings was modulated with an external rotating magnetic field (a dynamic assay with magnetic perturbation), which led to fluctuating extinction on UV-Vis spectroscopy measurement. As the surfaces of Au NRs were modified with antigens and antibodies, their periodic profile of extinction changed in accordance with surface modification of Au NRs. Read More

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http://dx.doi.org/10.1021/acs.analchem.0c02065DOI Listing

Gold Nanoparticles as Colorimetric Sensors for the Detection of DNA Bases and Related Compounds.

Authors:
Emilia Iglesias

Molecules 2020 Jun 23;25(12). Epub 2020 Jun 23.

Departamento de Química. Facultad de Ciencias. Campus A Zapateira, Universidade A Coruña, 15008-La Coruña, Spain.

Results regarding interaction of colloidal gold solutions with nucleobases, including uracil (U), as well as its sulfur derivatives, 2-thiouracil (2TU) and 4-thiouracil (4TU), cytosine (C), adenine (A), and guanine (G), as well as urea and thiourea (TU), are reported. Anionic stabilized citrate gold nanoparticles (AuNPs) were synthesized by reducing the tetrachloroaurate (III) trihydrate with trisodium citrate. The surface plasmon resonance (SPR) band was used in the characterization of synthesized AuNPs, as well as transmission electron microscope (TEM) imaging, which was used in the characterization of dispersed and aggregated gold nanoparticles. Read More

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http://dx.doi.org/10.3390/molecules25122890DOI Listing

Biosensors-Recent Advances and Future Challenges in Electrode Materials.

Sensors (Basel) 2020 Jun 23;20(12). Epub 2020 Jun 23.

Department of Chemical Sciences and Bernal Institute, University of Limerick, V94 T9PX Limerick, Ireland.

Electrochemical biosensors benefit from the simplicity, sensitivity, and rapid response of electroanalytical devices coupled with the selectivity of biorecognition molecules. The implementation of electrochemical biosensors in a clinical analysis can provide a sensitive and rapid response for the analysis of biomarkers, with the most successful being glucose sensors for diabetes patients. This review summarizes recent work on the use of structured materials such as nanoporous metals, graphene, carbon nanotubes, and ordered mesoporous carbon for biosensing applications. Read More

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http://dx.doi.org/10.3390/s20123561DOI Listing

A practical method for fabricating superparamagnetic films and the mechanism involved.

Nanoscale 2020 Jun 25. Epub 2020 Jun 25.

Department of Physics, National Taiwan Normal University, Taipei 116, Taiwan.

Due to the widespread applications of biosensors, such as in magnetic resonance imaging, cancer detection and drug delivery, the use of superparamagnetic materials for preparing biosensors has increased greatly. We report herein on a strategy toward fabrication of a nanoscale biosensor composed of superparamagnetic films. On increasing the film thickness of magnetic layers, a phase transition typically occurs from either a low-Curie-temperature state or a superparamagnetic state to a ferromagnetic state. Read More

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http://dx.doi.org/10.1039/c9nr10053aDOI Listing

Bioluminescence-Triggered Photoswitchable Bacterial Adhesions enable Higher Sensitivity and Dual-readout Bacterial Biosensors for Mercury.

ACS Sens 2020 Jun 25. Epub 2020 Jun 25.

We present a new concept for whole-cell biosensors that cou-ples the response to Hg with bioluminescence and bacterial aggregation. This allowed us to use the bacterial aggregation to preconcentrate the bioluminescent bacteria at the substrate surface and increase the sensitivity of Hg detection. This whole-cell biosensor combines a Hg-sensitive bioluminescence reporter and light-responsive bacterial cell-cell adhesions. Read More

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http://dx.doi.org/10.1021/acssensors.0c00855DOI Listing

Ratiometric Nanoparticle Probe Based on FRET-Amplified Phosphorescence for Oxygen Sensing with Minimal Phototoxicity.

Small 2020 Jun 25:e2002494. Epub 2020 Jun 25.

Laboratoire de Bioimagerie et Pathologies, UMR 7021 CNRS, Faculté de Pharmacie, Université de Strasbourg, Strasbourg, CS, 60024, France.

Luminescent oxygen probes enable direct imaging of hypoxic conditions in cells and tissues, which are associated with a variety of diseases, including cancer. Here, a nanoparticle probe that addresses key challenges in the field is developed, it: i) strongly amplifies room temperature phosphorescence of encapsulated oxygen-sensitive dyes; ii) provides ratiometric response to oxygen; and iii) solves the fundamental problem of phototoxicity of phosphorescent sensors. The nanoprobe is based on 40 nm polymeric nanoparticles, encapsulating ≈2000 blue-emitting cyanine dyes with fluorinated tetraphenylborate counterions, which are as bright as 70 quantum dots (QD525). Read More

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http://dx.doi.org/10.1002/smll.202002494DOI Listing

Synthesized Geopolymers Adsorb Bacterial Proteins, Toxins, and Cells.

Front Bioeng Biotechnol 2020 3;8:527. Epub 2020 Jun 3.

Center for Immunotherapy, Vaccines and Virotherapy, The Biodesign Institute, Arizona State University, Tempe, AZ, United States.

Pore-forming and hemolytic toxins are bacterial cytotoxic proteins required for virulence in many pathogens, including staphylococci and streptococci, and are notably associated with clinical manifestations of disease. Inspired by adsorption properties of naturally occurring aluminosilicates, we engineered inexpensive, laboratory-synthesized, aluminosilicate geopolymers with controllable pore and surface characteristics to remove pathogenic or cytotoxic material from the surrounding environment. In this study, macroporous and mesoporous geopolymers were produced with and without stearic acid surface modifications. Read More

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http://dx.doi.org/10.3389/fbioe.2020.00527DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7283576PMC

A Novel Nanoprobe Based on Core-Shell Au@Pt@Mesoporous SiO Nanozyme With Enhanced Activity and Stability for Mumps Virus Diagnosis.

Front Chem 2020 5;8:463. Epub 2020 Jun 5.

CAS Key Laboratory of Standardization and Measurement for Nanotechnology, CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology, Beijing, China.

Nanoporous materials with core-shell structure have received lots of attention owing to the great versatility of the functional cores and shells and their potential application in catalysis and biological applications. In this work, a facile method has been developed to synthesize uniform Au@Pt@mesoporous SiO nanostructures with high peroxidase-like activity, which had a well-defined core-shell structure with Au nanorods@Pt nanodots as a core and mesoporous SiO as a shell. The mesoporous SiO shell can not only provide convenient transmission channels but offer a substantial location for accommodation of large biomolecules, such as antibodies and antigens. Read More

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http://dx.doi.org/10.3389/fchem.2020.00463DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7290063PMC

Electrochemical impedimetric biosensors for food safety.

Food Sci Biotechnol 2020 Jul 10;29(7):879-887. Epub 2020 Jun 10.

Department of Food and Nutrition, Kookmin University, Seoul, 02707 Republic of Korea.

Electrochemical impedimetric biosensors (EIBs) have a simple structure and can be used to rapidly and sensitively detect and measure hazards in food. EIBs detect and measure target molecules by transducing biochemical reactions on their surface to electrical signal outputs responding to a sinusoidal electrical signal input. Due to their structural simplicity and analytical sensitivity, EIBs are regarded as the most potent method of food hazard monitoring that can be implemented in the food supply chain. Read More

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http://dx.doi.org/10.1007/s10068-020-00776-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7297935PMC

Bioprospecting Microbial Diversity for Lignin Valorization: Dry and Wet Screening Methods.

Front Microbiol 2020 9;11:1081. Epub 2020 Jun 9.

Department of Genomic Science and Biotechnology, Universidade Católica de Brasília - UCB, Brasília, Brazil.

Lignin is an abundant cell wall component, and it has been used mainly for generating steam and electricity. Nevertheless, lignin valorization, i.e. Read More

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http://dx.doi.org/10.3389/fmicb.2020.01081DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7295907PMC