54,947 results match your criteria Biosensors & Bioelectronics [Journal]


Multitechnique investigation into the aqueous behavior of plasma polymers.

Biointerphases 2018 Dec 5;13(6):06E410. Epub 2018 Dec 5.

ANFF-Vic Biointerface Engineering Hub, Faculty of Science, Engineering and Technology, Swinburne University of Technology, Hawthorn, Victoria 3122, Australia.

Plasma polymers are often used in applications requiring aqueous immersion; therefore, it is important to understand how this exposure affects the physical and chemical properties of the films. Three different plasma polymer films were deposited at different distances from the electrode, and the film properties were characterized using contact angle, ellipsometry, and x-ray photoelectron spectroscopy. The film behaviors in aqueous solutions were studied via quartz crystal microbalance with dissipation (QCM-D). Read More

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December 2018

Evaluation of Continuous Lactate Monitoring Systems within a Heparinized In Vivo Porcine Model Intravenously and Subcutaneously.

Biosensors (Basel) 2018 Dec 4;8(4). Epub 2018 Dec 4.

Biocrede Inc., Plymouth, MI 48170, USA.

We present an animal model used to evaluate the in vivo performance of electrochemical amperometric continuous lactate sensors compared to blood gas instruments. Electrochemical lactate sensors were fabricated, placed into 5 Fr central venous catheters (CVCs), and paired with wireless potentiostat devices. Following in vivo evaluation and calibration, sensors were placed within the jugular and femoral veins of a porcine subject as a preliminary assessment of in vivo measurement accuracy. Read More

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December 2018

Characterization of Recombinant His-Tag Protein Immobilized onto Functionalized Gold Nanoparticles.

Sensors (Basel) 2018 Dec 4;18(12). Epub 2018 Dec 4.

Department of Chemistry, University of Puerto Rico-Mayagüez Campus, Mayagüez 00680, Puerto Rico.

The recombinant polyhistidine-tagged hemoglobin I ((His)₆-rHbI) from the bivalve is an ideal biocomponent for a hydrogen sulfide (H₂S) biosensor due to its high affinity for H₂S. In this work, we immobilized (His)₆-rHbI over a surface modified with gold nanoparticles functionalized with 3-mercaptopropionic acid complexed with nickel ion. The attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR) analysis of the modified-gold electrode displays amide I and amide II bands characteristic of a primarily α-helix structure verifying the presence of (His)₆-rHbI on the electrode surface. Read More

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December 2018

Tracking antigen specific T-cells: Technological advancement and limitations.

Biotechnol Adv 2018 Nov 30. Epub 2018 Nov 30.

Centre for Personalised Nanomedicine, Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, QLD 4072, Australia; School of Chemistry and Molecular Biosciences, The University of Queensland, QLD 4072, Australia. Electronic address:

Assessing T-cell mediated immune status can help to understand the body's response to disease and also provide essential diagnostic information. However, detection and characterization of immune response are challenging due to the rarity of signature biomolecules in biological fluid and require highly sensitive and specific assay technique for the analysis. Until now, several techniques spanning from flow cytometry to microsensors have been developed or under investigation for T-cell mediated immune response monitoring. Read More

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November 2018

Diagnosis of hepatitis via nanomaterial-based electrochemical, optical or piezoelectrical biosensors: a review on recent advancements.

Mikrochim Acta 2018 Dec 1;185(12):568. Epub 2018 Dec 1.

Pharmaceutical Analysis Research Center, Tabriz University of Medical Sciences, Tabriz, 51664, Iran.

This review (with 145 refs.) summarizes the progress made in the past years in the field of nanomaterial based rapid detection of hepatitis viruses. Following an introduction into the field (with subsection on the significance of hepatitis and on fundamentals of biosensors), conventional methods for detection of hepatitis are discussed, along with their limitationss. Read More

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December 2018
1 Read

Concanavalin A-sugar affinity based system: Binding interactions, principle of glucose-responsiveness, and modulated insulin release for diabetes care.

Int J Biol Macromol 2018 Nov 28. Epub 2018 Nov 28.

Division of Biomedical Engineering, University of Saskatchewan, Saskatoon, Canada. Electronic address:

The glucose-responsive behavior of the Con A-sugar affinity based hydrogels, microgels, films and nanoparticles has been widely investigated in the application of drug delivery and biosensors. However, the principle or mechanism of such systems is not well known in literature. In this study, for the first time, the glucose-responsive principle (or principle) of the Con A-sugar based system was detailedly explained through the binding interactions test by isothermal titration calorimetry and ligand binding theory. Read More

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November 2018

Mesoporous silica nanoparticles: a versatile platform for biomedical applications.

Recent Pat Drug Deliv Formul 2018 Dec 3. Epub 2018 Dec 3.

Laboratory of Nanomedicine, Institute of Life Sciences, Nalco Square, Bhubaneswar -751023, Odisha. India.

Background: Nanotechnology based drug delivery approach has emerged as a promising field, where different kinds of formulations are developed for therapeutic applications. Inflowing of nanomedicine developed through various biomaterials appears to be a game changer in the domain of pharmaceutical and biotechnological industries. Nanomedicines propose to overcome the major constraints of conventional medicine of low solubility and stability, and non-adequate pharmacokinetic profiles and side effects. Read More

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December 2018

Facile preparation of a bacteria imprinted artificial receptor for highly-selective bacterial recognition and label-free impedimetric detection.

Anal Chem 2018 Dec 3. Epub 2018 Dec 3.

The effective identification and quantification of pathogenic bacteria is essential for addressing serious public health issues. Here we demonstrate a simple and universal impedimetric sensor for highly selective and sensitive detection of pathogenic bacteria based on the recognition by a bacteria-imprinted polypyrrole (BIP) film. The BIP film was facilely prepared via one-step electro-polymerization followed by in-situ removal of the bacterial template. Read More

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December 2018

Assessing the Potential Deployment of Biosensors for Point-of-Care Diagnostics in Developing Countries: Technological, Economic and Regulatory Aspects.

Biosensors (Basel) 2018 Nov 29;8(4). Epub 2018 Nov 29.

Energy Center, Ecole Polytechnique Fédérale de Lausanne, 1015 Lausanne, Switzerland.

Infectious diseases and antimicrobial resistance are major burdens in developing countries, where very specific conditions impede the deployment of established medical infrastructures. Since biosensing devices are nowadays very common in developed countries, particularly in the field of diagnostics, they are at a stage of maturity at which other potential outcomes can be explored, especially on their possibilities for multiplexing and automation to reduce the time-to-results. However, the translation is far from being trivial. Read More

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November 2018

RNA-DNA fibers and polygons with controlled immunorecognition activate RNAi, FRET and transcriptional regulation of NF-κB in human cells.

Nucleic Acids Res 2018 Dec 4. Epub 2018 Dec 4.

Nanoscale Science Program, Department of Chemistry, University of North Carolina at Charlotte, Charlotte, NC 28223, USA.

Nucleic acid-based assemblies that interact with each other and further communicate with the cellular machinery in a controlled manner represent a new class of reconfigurable materials that can overcome limitations of traditional biochemical approaches and improve the potential therapeutic utility of nucleic acids. This notion enables the development of novel biocompatible 'smart' devices and biosensors with precisely controlled physicochemical and biological properties. We extend this novel concept by designing RNA-DNA fibers and polygons that are able to cooperate in different human cell lines and that have defined immunostimulatory properties confirmed by ex vivo experiments. Read More

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December 2018

Recent advances in biosensors for diagnosis of celiac disease: A review.

Biotechnol Bioeng 2018 Dec 5. Epub 2018 Dec 5.

Faculty of Applied Sciences and Biotechnology, Shoolini University, Solan, India.

Celiac disease (CD) is an intestinal issue activated by the inappropriate immune reaction towards gluten protein of wheat, rye, barley, oats, and autoantigen, tissue transglutaminase. Regardless of the accessibility of immunochemical conventions for research facility analysis of CD, there is as yet a need of speedier, less expensive, and simpler devices for diagnosing CD. This review concentrates on progresses in biosensors for diagnosing CD in perspective of the scaled down hardware, multianalyte discovery and low sample volume necessity. Read More

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December 2018
1 Read

DNA-functionalized Nanoceria for Probing Oxidation of Phosphorus Compounds.

Langmuir 2018 Dec 5. Epub 2018 Dec 5.

Chemical reactions without an obvious optical signal change, such as fluorescence or color, are difficult to monitor. Often, more advanced analytical techniques such as HPLC and mass spectroscopy are needed. It would be useful to convert such reactions to changes in optical signals. Read More

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December 2018

Functional selectivity profiling of the angiotensin II type 1 receptor using pathway-wide BRET signaling sensors.

Sci Signal 2018 Dec 4;11(559). Epub 2018 Dec 4.

Department of Medicine, Research Institute of the McGill University Health Center (RI-MUHC), McGill University, Montréal, QC H4A 3J1, Canada.

G protein-coupled receptors (GPCRs) are important therapeutic targets that exhibit functional selectivity (biased signaling), in which different ligands or receptor variants elicit distinct downstream signaling. Understanding all the signaling events and biases that contribute to both the beneficial and adverse effects of GPCR stimulation by given ligands is important for drug discovery. Here, we report the design, validation, and use of pathway-selective bioluminescence resonance energy transfer (BRET) biosensors that monitor the engagement and activation of signaling effectors downstream of G proteins, including protein kinase C (PKC), phospholipase C (PLC), p63RhoGEF, and Rho. Read More

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December 2018

Platforms for Bioorthogonal Oligonucleotide-templated Reactions: Translating Concepts into Devices.

Chimia (Aarau) 2018 Nov;72(11):809-814

Department of Bioengineering Imperial College London London SW7 2AZ, UK;, Email:

The exponential improvements made in DNA sequencing technologies, together with the rapidly declining associated costs, has increasingly led to the expansion of the field of personalised genomic medicine. Changes in the sequence or copy number of specific deoxyribonucleic acid (DNA) or ribonucleic acid (RNA) molecules represent key signatures for the diagnosis, prognosis, classification and monitoring of a broad range of pathologies, most notably cancer. Technologies that can detect these changes require analytical tools that can detect DNA or RNA with high sensitivity and high specificity. Read More

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November 2018

Non-Invasive Diagnosis of Diabetes by Volatile Organic Compounds in Urine Using FAIMS and Fox4000 Electronic Nose.

Biosensors (Basel) 2018 Dec 1;8(4). Epub 2018 Dec 1.

School of Engineering, University of Warwick, Coventry CV4 7AL, UK.

The electronic nose (eNose) is an instrument designed to mimic the human olfactory system. Usage of eNose in medical applications is more popular than ever, due to its low costs and non-invasive nature. The eNose sniffs the gases and vapours that emanate from human waste (urine, breath, and stool) for the diagnosis of variety of diseases. Read More

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December 2018

Chiral Plasmonic Biosensors.

Biosensors (Basel) 2018 Dec 1;8(4). Epub 2018 Dec 1.

Chemistry Department of Lomonosov, Moscow State University, 119991 Moskva, Russia.

Biosensing requires fast, selective, and highly sensitive real-time detection of biomolecules using efficient simple-to-use techniques. Due to a unique capability to focus light at nanoscale, plasmonic nanostructures provide an excellent platform for label-free detection of molecular adsorption by sensing tiny changes in the local refractive index or by enhancing the light-induced processes in adjacent biomolecules. This review discusses the opportunities provided by surface plasmon resonance in probing the chirality of biomolecules as well as their conformations and orientations. Read More

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December 2018

Label-Free and Redox Markers-Based Electrochemical Aptasensors for Aflatoxin M1 Detection.

Sensors (Basel) 2018 Dec 1;18(12). Epub 2018 Dec 1.

Department of Nuclear Physics and Biophysics, Comenius University, Mlynska dolina F1, 842 48 Bratislava, Slovakia.

We performed a comparative analysis of the sensitivity of aptamer-based biosensors for detection mycotoxin aflatoxin M₁ (AFM1) depending on the method of immobilization of DNA aptamers and method of the detection. Label-free electrochemical impedance spectroscopy (EIS) and differential pulse voltammetry (DPV) for ferrocene labeled neutravidin layers were used for this purpose. Amino-modified DNA aptamers have been immobilized at the surface of polyamidoamine dendrimers (PAMAM) of fourth generation (G4) or biotin-modified aptamers were immobilized at the neutravidin layer chemisorbed at gold surface. Read More

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December 2018
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Electrodeposition⁻Assisted Assembled Multilayer Films of Gold Nanoparticles and Glucose Oxidase onto Polypyrrole-Reduced Graphene Oxide Matrix and Their Electrocatalytic Activity toward Glucose.

Nanomaterials (Basel) 2018 Dec 1;8(12). Epub 2018 Dec 1.

College of Life Sciences, South China Normal University, Guangzhou 510631, China.

The study reports a facile and eco-friendly approach for nanomaterial synthesis and enzyme immobilization. A corresponding glucose biosensor was fabricated by immobilizing the gold nanoparticles (AuNPs) and glucose oxidase (GOD) multilayer films onto the polypyrrole (PPy)/reduced graphene oxide (RGO) modified glassy carbon electrode (GCE) via the electrodeposition and self-assembly. PPy and graphene oxide were first coated on the surface of a bare GCE by the electrodeposition. Read More

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December 2018

Novel grafted electrochemical interface for covalent glucose oxidase immobilization using reactive pentafluorophenyl methacrylate.

Colloids Surf B Biointerfaces 2018 Nov 28;175:1-9. Epub 2018 Nov 28.

Electrochemical Methods Laboratory - Analytical and Applied Chemistry Department at Institut Químic de Sarrià, Universitat Ramon Llull, Via Augusta, 390, 08017, Barcelona, Spain. Electronic address:

One of the most important factors for the proper functioning of enzymatic electrochemical biosensors is the enzyme immobilization strategy. In this work, glucose oxidase was covalently immobilized using pentafluorophenyl methacrylate (PFM) by applying two different surface modification techniques (plasma polymerization and plasma-grafting). The grafted surface was specifically designed to covalently anchor enzyme molecules. Read More

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November 2018
1 Read

Multisegment nanowire/nanoparticle hybrid arrays as electrochemical biosensors for simultaneous detection of antibiotics.

Biosens Bioelectron 2018 Oct 17;126:632-639. Epub 2018 Oct 17.

Department of Chemical Engineering, University of Massachusetts Lowell, One University Ave, Lowell, MA 01854, USA. Electronic address:

Antibiotics such as penicillin and tetracycline drugs are widely used in food animals to treat, control, and prevent diseases, and penicillin is approved for use to improve growth rates in pigs and poultry. However, due to the overuse of antibiotics in food and medical industry, the antimicrobial resistance is starting to show up in some developing countries. The antibiotic abuse may cause allergic reactions, resistance in microorganisms and general lowering of immunity in consumers of meat and dairy products. Read More

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October 2018
3 Reads

Bioassay of saliva proteins: The best alternative for conventional methods in non-invasive diagnosis of cancer.

Int J Biol Macromol 2018 Dec 1. Epub 2018 Dec 1.

Dental and Periodontal Research Center, Tabriz University of Medical Sciences, Tabriz, Iran; Students' Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran. Electronic address:

Non-invasive diagnosis of cancer is often the key to effective treatment and patient survival. Saliva as a multi-constituent oral fluid comprises various disease signaling biomarkers, holds great potential for early-stage cancer diagnostics with cost-effective and easy collection, storage, transport and processing. Therefore, detection of biomarkers and proteins in the saliva samples is highly demand. Read More

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December 2018
2 Reads

Label-free plasmonic biosensors for point-of-care diagnostics: a review.

Expert Rev Mol Diagn 2018 Dec 4. Epub 2018 Dec 4.

a Nanobiosensors and Bioanalytical Applications Group , Catalan Institute of Nanoscience and Nanotechnology (ICN2), CSIC, BIST and CIBER-BBN , Campus UAB, Ed-ICN2, 08193 Bellaterra , Barcelona , Spain .

Introduction: Optical biosensors and particularly those based on nanoplasmonics technology have emerged in recent decades as a potential solution for disease diagnostics and therapy follow-up at the point-of-care. These biosensor platforms could overcome some of the challenges faced in conventional diagnosis techniques offering label-free assays with immediate results and employing small and user-friendly devices. Areas covered: In this review, we will provide a critical overview of the recent advances in the development of nanoplasmonic biosensors for point-of-care diagnostics. Read More

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December 2018
2 Reads

Shining light on plant hormones with genetically encoded biosensors.

Biol Chem 2018 Dec 4. Epub 2018 Dec 4.

Laboratoire Reproduction et Développement des Plantes, Université Lyon, ENS de Lyon, UCB Lyon 1, CNRS, INRA, F-69342 Lyon, France.

Signalling molecules are produced, degraded, modified and transported throughout the development of higher organisms. Understanding their mode of action implies understanding these dynamics in vivo and in real time. Genetically encoded biosensors are being more and more used as tools to 'follow' signalling molecules and their responses inside an organism. Read More

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December 2018
3 Reads

Biomedical Applications of Layer-by-Layer Self-Assembly for Cell Encapsulation: Current Status and Future Perspectives.

Adv Healthc Mater 2018 Dec 4:e1800939. Epub 2018 Dec 4.

Institute of Burn Research, State Key Laboratory of Trauma, Burn and Combined Injury, Southwest Hospital, Third Military Medical University (Army Medical University), Gaotanyan Street, Chongqing, 400038, China.

Encapsulating living cells within multilayer functional shells is a crucial extension of cellular functions and a further development of cell surface engineering. In the last decade, cell encapsulation has been widely utilized in many cutting-edge biomedical fields. Compared with other techniques for cell encapsulation, layer-by-layer (LbL) self-assembly technology, due to the versatility and tunability to fabricate diverse multilayer shells with controllable compositions and structures, is considered as a promising approach for cell encapsulation. Read More

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December 2018

Liquid biopsy of circulating tumor DNA and biosensor applications.

Biosens Bioelectron 2018 Nov 22;126:596-607. Epub 2018 Nov 22.

Hubei Province Key Laboratory of Occupational Hazard Identification and Control; School of Public Health, Medical College; Wuhan University of Science and Technology, Wuhan 430065, PR China. Electronic address:

Circulating tumor DNA (ctDNA) as a class of liquid biopsy is a type of gene fragment that contains tumor-specific gene changes in body fluids such as human peripheral blood. More and more evidences show that ctDNA is an excellent tumor biomarker for diagnosis, prognosis, tumor heterogeneity and so on. ctDNA is a tumor code in the blood. Read More

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November 2018

Understanding the size effect of graphene quantum dots on protein adsorption.

Colloids Surf B Biointerfaces 2018 Nov 24;174:575-581. Epub 2018 Nov 24.

College of Life Information Science and Instrument Engineering, Hangzhou Dianzi University, Hangzhou, 310018, People's Republic of China. Electronic address:

Due to the unique structural and mechanical properties, graphene quantum dots (GQDs) are considered as potential candidates in the field of biosensors, bioimaging, and drug delivery etc. In this work, the adsorption of protein villin headpiece (HP35) on GQDs with different sizes was investigated by molecular dynamics simulations. The simulation results identified the key role of the π-π stacking interactions between the aromatic residues of HP35 and GQDs as the binding site. Read More

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November 2018

Ochratoxin A detection in coffee by competitive inhibition assay using chitosan-based surface plasmon resonance compact system.

Colloids Surf B Biointerfaces 2018 Nov 26;174:569-574. Epub 2018 Nov 26.

Food Engineering and Bioprocess Technology, Department of Food, Agriculture and Bioresources, Asian Institute of Technology, Pathumthani 12120, Thailand. Electronic address:

This study demonstrates the evaluation of ochratoxin A (OTA) in coffee on compact surface plasmon resonance (SPR) biosensors based on crosslinked chitosan and carboxymethyl chitosan nanomatrix substrates. Ochratoxin A is a toxic secondary metabolite widely produced by Aspergillus and Penicillium fungi and requires regular quantification and detection in food samples. The gold coated SPR chips were synthesized with chitosan and carboxymethyl chitosan through spin coating technique. Read More

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November 2018

Single-Cell Imaging of Metastatic Potential of Cancer Cells.

iScience 2018 Nov 15;10:53-65. Epub 2018 Nov 15.

Department of Medicine, University of California at San Diego, George Palade Labs, Room 232, La Jolla, CA 92093-0651, USA; Department of Cellular and Molecular Medicine, University of California at San Diego, La Jolla, CA 92093-0651, USA; Moores Cancer Center, University of California, San Diego, La Jolla, CA, USA. Electronic address:

Molecular imaging of metastatic "potential" is an unvanquished challenge. To engineer biosensors that can detect and measure the metastatic "potential" of single living cancer cells, we carried out a comprehensive analysis of the pan-cancer phosphoproteome to search for actin remodelers required for cell migration, which are enriched in cancers but excluded in normal cells. Only one phosphoprotein emerged, tyr-phosphorylated CCDC88A (GIV/Girdin), a bona fide metastasis-related protein across a variety of solid tumors. Read More

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November 2018

Peri/epicellular Protein Disulfide Isomerase-A1 Acts as an Upstream Organizer of Cytoskeletal Mechanoadaptation in Vascular Smooth Muscle Cells.

Am J Physiol Heart Circ Physiol 2018 Nov 30. Epub 2018 Nov 30.

Heart Institute, University of Sao Paulo, Brazil.

Although redox processes closely interplay with mechanoresponses to control vascular remodeling, redox pathways coupling mechanostimulation to cellular cytoskeletal organization remain unclear. The peri/epicellular pool of protein disulfide isomerase-A1(pecPDIA1) supports post-injury vessel remodeling. Using distinct models, we interrogated whether pecPDIA1 could work as a redox-dependent organizer of cytoskeletal mechanoresponses. Read More

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November 2018

Amorphous metal boride as a novel platform for acetylcholinesterase biosensor development and detection of organophosphate pesticides.

Nanotechnology 2019 Feb 30;30(5):055501. Epub 2018 Nov 30.

Key Laboratory of Applied Chemistry, Department of Applied Chemistry, Yanshan University, Qinhuangdao 066004, People's Republic of China.

The exploration of new materials for modifying electrodes is important to advance electrochemical biosensors. Herein, we demonstrated that amorphous bimetallic boride material (Co-2Ni-B) prepared by a simple and facile aqueous reaction is an efficient matrix to immobilize acetylcholinesterase (AChE) to construct a biosensor for the determination of organophosphate pesticides. The effects of different composition and crystallinity on its electrochemical performance are investigated, and the optimization studies of the biological transducer were also discussed. Read More

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February 2019

Harnessing a previously unidentified capability of bacterial allosteric transcription factors for sensing diverse small molecules in vitro.

Sci Adv 2018 Nov 28;4(11):eaau4602. Epub 2018 Nov 28.

State Key Laboratory of Microbial Resources and Chinese Academy of Sciences (CAS) Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, CAS, Beijing 100101, P.R. China.

A plethora of bacterial allosteric transcription factors (aTFs) have been identified to sense a variety of small molecules. Introduction of a novel aTF-based approach to sense diverse small molecules in vitro will signify a broad series of detection applications. Here, we found that aTFs could interact with their nicked DNA binding sites. Read More

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November 2018

Antiplatelet agents in uncertain clinical scenarios-a bleeding nightmare.

Cardiovasc Diagn Ther 2018 Oct;8(5):647-662

Department of Cardiology, Altnagelvin Area Hospital, Western Health and Social Care Trust, Derry/Londonderry, Northern Ireland, UK.

Despite over 40 years since the first percutaneous coronary intervention (PCI) was performed, the optimal dual antiplatelet therapy (DAPT) regime poses a significant challenge for clinicians, especially in certain scenarios. DAPT is the standard of care in PCI following an acute coronary syndrome (ACS) or for elective patients with obstructive coronary artery disease (CAD). There remains significant uncertainty regarding DAPT in patients at high risk of bleeding, such as the elderly and patients requiring anticoagulation. Read More

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October 2018

Diabetes and antiplatelet therapy: from bench to bedside.

Cardiovasc Diagn Ther 2018 Oct;8(5):594-609

Division of Cardiology, University of Florida College of Medicine-Jacksonville, Jacksonville, Florida, USA.

Diabetes mellitus (DM) is a metabolic disorder associated with accelerated atherogenesis and an increased risk of atherothrombotic complications. Multiple mechanisms contribute to the pro-thrombotic status which characterizes DM patients underscoring the importance of antiplatelet therapies used for secondary prevention in these patients. For many years, dual antiplatelet therapy (DAPT) with aspirin and the P2Y inhibitor clopidogrel has represented the mainstay of treatment following an acute coronary syndrome (ACS) or in patients undergoing percutaneous coronary interventions (PCI). Read More

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October 2018

Early detection of cervical cancer based on high-risk HPV DNA-based genosensors: A systematic review.

Biofactors 2018 Nov 29. Epub 2018 Nov 29.

Department of Modern Sciences and Technologies, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.

Human papillomavirus type (HPV) is a common cause of sexually transmitted disease (STD) in humans. HPV types 16 and 18 as the highest risk types are related with gynecologic malignancy and cervical cancer (CC) among women worldwide. Recently, considerable development of genosensors, which allows dynamic monitoring of hybridization events for HPV-16 and 18, has been a topic of focus by many researchers. Read More

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November 2018
1 Read

Remote Surveillance Technologies: Realizing the Aim of Right Patient, Right Data, Right Time.

Anesth Analg 2018 Nov 21. Epub 2018 Nov 21.

From the Department of Anesthesia, Critical Care, and Pain Medicine, Massachusetts General Hospital, Boston, Massachusetts.

The convergence of multiple recent developments in health care information technology and monitoring devices has made possible the creation of remote patient surveillance systems that increase the timeliness and quality of patient care. More convenient, less invasive monitoring devices, including patches, wearables, and biosensors, now allow for continuous physiological data to be gleaned from patients in a variety of care settings across the perioperative experience. These data can be bound into a single data repository, creating so-called data lakes. Read More

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November 2018

Recent Development of AIEgens/nucleic Acid Nanostructure for Bioanalytical Application.

Chem Asian J 2018 Nov 29. Epub 2018 Nov 29.

CHINA.

DNA occupies significant roles in life processes which include encoding the sequences of proteins and transferring accurately genetic information from generation to generation. Recent discoveries have demonstrated that a variety of biologically functions are correlated with DNA's conformational transition. The non-B form has attained great attention among the diverse forms of DNA during the past several years. Read More

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November 2018
1 Read

Advances on Aptamers against Protozoan Parasites.

Genes (Basel) 2018 Nov 28;9(12). Epub 2018 Nov 28.

Sección de Estudios de Posgrado e Investigación, ENMH, Instituto Politécnico Nacional, Ciudad de México 07738, Mexico.

Aptamers are single-stranded DNA or RNA sequences with a unique three-dimensional structure that allows them to recognize a particular target with high affinity. Although their specific recognition activity could make them similar to monoclonal antibodies, their ability to bind to a large range of non-immunogenic targets greatly expands their potential as tools for diagnosis, therapeutic agents, detection of food risks, biosensors, detection of toxins, drug carriers, and nanoparticle markers, among others. One aptamer named Pegaptanib is currently used for treating macular degeneration associated with age, and many other aptamers are in different clinical stages of development of evaluation for various human diseases. Read More

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November 2018

Manganese Doped Hydroxyapatite Nanoparticles Based Enzyme-Less Electrochemical Sensor for Detecting Hydroquinone.

J Nanosci Nanotechnol 2019 Apr;19(4):2034-2043

Department of Electronic Engineering, Chemistry and Materials Engineering, University of Messina, Messina 98166, Italy.

Manganese (Mn) doped hydroxyapatite (HA) nanoparticles were synthesized by a simple microwave irradiation method and characterized using XRD, SEM, micro-Raman, FTIR, XPS and vibrating sample magnetometer (VSM) methods. The as prepared 3 M% Mn doped HA (3 M% Mn-HA) nanoparticles modified glassy carbon electrode (GCE) showed an excellent electrocatalytic activity towards the oxidation of hydroquinone (HQ). The electrochemical studies demonstrated that the 3 M% Mn-HA nanoparticles modified GCE detects HQ linearly over a wide concentration range of 1. Read More

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Microwave Spectroscopic Detection of Human Hsp70 Protein on Annealed Gold Nanostructures on ITO Glass Strips.

Biosensors (Basel) 2018 Nov 27;8(4). Epub 2018 Nov 27.

Laboratory Interdisciplinaire Carnot de Bourgogne (ICB), UMR-CNRS 6303, University of Bourgogne Franche-Comté, 9 Avenue Alain Savary, 21078 Dijon CEDEX, France.

Conductive indium-tin oxide (ITO) and non-conductive glass substrates were successfully modified with embedded gold nanoparticles (AuNPs) formed by controlled thermal annealing at 550 °C for 8 h in a preselected oven. The authors characterized the formation of AuNPs using two microscopic techniques: scanning electron microscopy (SEM) and atomic force microscopy (AFM). The analytical performances of the nanostructured-glasses were compared regarding biosensing of Hsp70, an ATP-driven molecular chaperone. Read More

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November 2018

Applications of Gold Nanoparticles in Non-Optical Biosensors.

Nanomaterials (Basel) 2018 Nov 26;8(12). Epub 2018 Nov 26.

Hunan Key Laboratory of Biomedical Nanomaterials and Devices, Hunan University of Technology, Zhuzhou 412007, China.

Due to their unique properties, such as good biocompatibility, excellent conductivity, effective catalysis, high density, and high surface-to-volume ratio, gold nanoparticles (AuNPs) are widely used in the field of bioassay. Mainly, AuNPs used in optical biosensors have been described in some reviews. In this review, we highlight recent advances in AuNP-based non-optical bioassays, including piezoelectric biosensor, electrochemical biosensor, and inductively coupled plasma mass spectrometry (ICP-MS) bio-detection. Read More

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November 2018

A Liposomal Platform for Sensing of Extracellular Analytes Near Cells.

Biosensors (Basel) 2018 Nov 26;8(4). Epub 2018 Nov 26.

ARC Centre of Excellence for Nanoscale BioPhotonics, Institute of Photonics and Advanced Sensing, Department of Chemistry, School of Physical Sciences, The University of Adelaide, Adelaide SA 5005, Australia.

Cell-permeable fluorescent chemosensors (calcein, monochlorobimane, and a recently reported spiropyran-based sensor SP2) have been incorporated into yeast total lipid extract-based liposomes to suppress inherent cell permeability to allow the detection of extracellular Ca, GSH, and Zn, respectively. The repurposed sensors have enhanced aqueous solubility and the ability to quantitatively measure biologically relevant concentrations of Ca (0.25 mM⁻1 mM), Zn (6. Read More

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November 2018
2 Reads

Nanofibers for Biomedical and Healthcare Applications.

Macromol Biosci 2018 Nov 28:e1800256. Epub 2018 Nov 28.

LGP2, Grenoble INP, CNRS, Université Grenoble Alpes, F-38000, Grenoble, France.

Unique features of nanofibers provide enormous potential in the field of biomedical and healthcare applications. Many studies have proven the extreme potential of nanofibers in front of current challenges in the medical and healthcare field. This review highlights the nanofiber technologies, unique properties, fabrication techniques (i. Read More

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November 2018

Peak emission wavelength and fluorescence lifetime are coupled in far-red, GFP-like fluorescent proteins.

PLoS One 2018 28;13(11):e0208075. Epub 2018 Nov 28.

Department of Biological Sciences, Sunnybrook Research Institute, Toronto, Ontario, Canada.

The discovery and use of fluorescent proteins revolutionized cell biology by allowing the visualization of proteins in living cells. Advances in fluorescent proteins, primarily through genetic engineering, have enabled more advanced analyses, including Förster resonance energy transfer (FRET) and fluorescence lifetime imaging microscopy (FLIM) and the development of genetically encoded fluorescent biosensors. These fluorescence protein-based sensors are highly effective in cells grown in monolayer cultures. Read More

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November 2018

Stable, Strain-Sensitivity Conductive Hydrogel with Anti-freezing Capable, Remoldability and Reusability.

ACS Appl Mater Interfaces 2018 Nov 28. Epub 2018 Nov 28.

Conductive hydrogels have important potential in biosensors, bioactuators and health recording electrodes, but they are often troubled by sensitivity, operating temperature range and whether they can be recycled or not. In this paper, conductive hydrogels polyvinyl alcohol/glycerol/polyaniline (PGA) were prepared by organic combination of low-cost polyvinyl alcohol (PVA), polyaniline (PANi)and glycerin. First, the effects of PVA, glycerol, aniline/phytic acid solution concentration on the mechanical properties, electrical properties and frost resistance of the PGA gel were discussed. Read More

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November 2018

Highly sensitive fluorescent bioassay of 2,3,7,8-tetrachloro-dibenzo-p-dioxin based on abnormal expression of cytochrome P450 1A2 in human cells.

Anal Chim Acta 2019 Jan 6;1046:179-184. Epub 2018 Aug 6.

Key Laboratory of Environmental Medicine Engineering, Ministry of Education, Jiangsu Engineering Laboratory of Smart Carbon-Rich Materials and Device, School of Chemistry and Chemical Engineering, Southeast University, Nanjing, 211189, PR China. Electronic address:

Current in vitro bioassays of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD, a major threat carcinogen) are relied on murine cells and fluorescent probe 7-ethoxyresorufin (7-ER), in which TCDD mostly causes abnormal expression of cytochrome P450 1A1 (CYP1A1). However, for human cells, TCDD mainly leads to a distinct abnormal expression of cytochrome P450 1A2 (CYP1A2). The poor response of 7-ER to CYP1A2 limits the traditional bioassay for human cells. Read More

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January 2019

A Novel Peptide Aptamer to Detect Lactate Dehydrogenase.

J Biomed Nanotechnol 2019 Jan;15(1):204-211

Alternative antibody (aptamer)-based biosensors are attracting increasing attention owing to advantages such as simplicity and low cost, which are beneficial for point-of-care diagnosis, particularly where resources are limited. In this study based on modeling predictions made with Autodock Vina, the binding affinity of an optimized novel peptide (Pf_P1: KITTTDEEVEGIFD) was altered compared to that of the original epitope peptide (P1: KITDEEVEGIFDC). The binding energy of Pf_P1 implies that it has stronger interactions with lactate dehydrogenase (LDH) than with human LDH. Read More

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January 2019
3 Reads

Coordinated histone modifications and chromatin reorganization in a single cell revealed by FRET biosensors.

Proc Natl Acad Sci U S A 2018 Nov 26. Epub 2018 Nov 26.

Department of Bioengineering, Institute of Engineering in Medicine, University of California, San Diego, La Jolla, CA 92093;

The dramatic reorganization of chromatin during mitosis is perhaps one of the most fundamental of all cell processes. It remains unclear how epigenetic histone modifications, despite their crucial roles in regulating chromatin architectures, are dynamically coordinated with chromatin reorganization in controlling this process. We have developed and characterized biosensors with high sensitivity and specificity based on fluorescence resonance energy transfer (FRET). Read More

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November 2018

Optical Biosensors for Label-Free Detection of Small Molecules.

Sensors (Basel) 2018 Nov 24;18(12). Epub 2018 Nov 24.

Departamento de Química Analítica, Facultad de Ciencias Químicas, Universidad Complutense de Madrid, E-28040 Madrid, Spain.

Label-free optical biosensors are an intriguing option for the analyses of many analytes, as they offer several advantages such as high sensitivity, direct and real-time measurement in addition to multiplexing capabilities. However, development of label-free optical biosensors for small molecules can be challenging as most of them are not naturally chromogenic or fluorescent, and in some cases, the sensor response is related to the size of the analyte. To overcome some of the limitations associated with the analysis of biologically, pharmacologically, or environmentally relevant compounds of low molecular weight, recent advances in the field have improved the detection of these analytes using outstanding methodology, instrumentation, recognition elements, or immobilization strategies. Read More

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November 2018
3 Reads

Cerium Oxide-Tungsten Oxide Core-Shell Nanowire-Based Microsensors Sensitive to Acetone.

Biosensors (Basel) 2018 Nov 23;8(4). Epub 2018 Nov 23.

Instituto de Microelectrónica de Barcelona (IMB-CNM, CSIC), Campus UAB, 08193 Bellaterra, Spain.

Gas sensitive cerium oxide-tungsten oxide core-shell nanowires are synthesized and integrated directly into micromachined platforms via aerosol assisted chemical vapor deposition. Tests to various volatile organic compounds (acetone, ethanol, and toluene) involved in early disease diagnosis demonstrate enhanced sensitivity to acetone for the core-shell structures in contrast to the non-modified materials (i.e. Read More

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November 2018

Use of Electronic Noses in Seawater Quality Monitoring: A Systematic Review.

Biosensors (Basel) 2018 Nov 23;8(4). Epub 2018 Nov 23.

National Research Council of Italy, Institute of Clinical Physiology (CNR-IFC), Via Moruzzi 1, 56124 Pisa, Italy.

Electronic nose (eNose) systems are particularly appreciated for their portability, usability, relative low cost, and real-time or near real-time response. Their application finds space in several domains, including environmental monitoring. Within this field, marine monitoring is of particular scientific relevance due to the fragility of this specific environment, daily threatened by human activities that can potentially bring to catastrophic and irreversible consequences on marine wildlife. Read More

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November 2018