63,655 results match your criteria Biosensors & bioelectronics[Journal]


Aptamers, the bivalent agents as probes and therapies for coronavirus infections: A systematic review.

Mol Cell Probes 2020 Jul 4:101636. Epub 2020 Jul 4.

Baqiyatallah Research Center for Gastroenterology and Liver Disease, Baqiyatallah University of Medical Sciences, Tehran, Iran. Electronic address:

The recently known coronavirus, SARS-CoV-2, has turn into the greatest global health challenge, affecting a large number of societies. The lack of specific treatment and gold-standard diagnostic system has made the situation more complicated. Efforts have led to production of several diagnostic kits that are associated with limitations such as inadequate sensitivity and accuracy. Read More

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http://dx.doi.org/10.1016/j.mcp.2020.101636DOI Listing

Facile strategy for immobilizing horseradish peroxidase on a novel acetate functionalized ionic liquid/MWCNT matrix for electrochemical biosensing.

Int J Biol Macromol 2020 Jul 4. Epub 2020 Jul 4.

Department of Chemistry, School of Advanced Sciences, Vellore Institute of Technology (VIT), Vellore 632014, India. Electronic address:

Facile yet simple platforms for the immobilization of biomolecules have always been a substantial requirement for the fabrication of proficient biosensors. In this study, we report a naphthyl substituted acetate functionalized ionic liquid (NpAc-IL) for the covalent anchoring of horseradish peroxidase (HRP), using which the direct electrochemistry of HRP was successfully accomplished and a HO biosensor was developed. The naphthyl substitution on the NpAc-IL was utilized for the π-π stacking with the MWCNT modified GCE and the terminal -OCH group of NpAc-IL was used for the covalent attachment with the free -NH group of HRP via amide bond formation. Read More

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http://dx.doi.org/10.1016/j.ijbiomac.2020.07.005DOI Listing

Revealing the Activity of Trimeric G-proteins in Live Cells with a Versatile Biosensor Design.

Cell 2020 Jun 27. Epub 2020 Jun 27.

Department of Biochemistry, Boston University School of Medicine, Boston, MA 02118, USA. Electronic address:

Heterotrimeric G-proteins (Gαβγ) are the main transducers of signals from GPCRs, mediating the action of countless natural stimuli and therapeutic agents. However, there are currently no robust approaches to directly measure the activity of endogenous G-proteins in cells. Here, we describe a suite of optical biosensors that detect endogenous active G-proteins with sub-second resolution in live cells. Read More

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http://dx.doi.org/10.1016/j.cell.2020.06.020DOI Listing

Activatable CRISPR Transcriptional Circuits Lighted up by Fluorescent RNA for mRNA Sensing and Silencing.

Angew Chem Int Ed Engl 2020 Jul 7. Epub 2020 Jul 7.

Institute of Chemical Biology & Nanomedicine, State Key Laboratory of Chemo/Biosensing & Chemometrics, College of Chemistry & Chemical Engineering, Hunan University, Changsha 410082, China, Yuelu, 410082, Changsha, CHINA.

CRISPR-dCas9 systems precisely activated with cell-specific information improve abilities to develop smart sensors or therapeutic strategies. We report the first development of an activatable dCas9 transcriptional circuit that enables sensing and silencing of mRNA in living cells using hybridization-mediated structure switching for gRNA activation. The gRNA is designed with the spacer sequence blocked by a hairpin structure, and mRNA hybridization induces gRNA structure switching and activates transcription of reporter RNA. Read More

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http://dx.doi.org/10.1002/anie.202004751DOI Listing

Subcellular Localization of PI3P in Arabidopsis.

Methods Mol Biol 2020 ;2177:109-118

Department of Biological Sciences, Pusan National University, Busan, Republic of Korea.

Phosphatidylinositol-3-phosphate (PI3P) is a signaling phospholipid enriched in the membranes of late endosomes (LE) and vacuoles. PI3P mediates vacuolar and endosomal trafficking through recruiting PI3P-binding effector proteins to the LE. PI3P is produced from phosphatidylinositol by the PI 3-kinase complex containing VACUOLAR PROTEIN SORTING 34 (VPS34). Read More

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http://dx.doi.org/10.1007/978-1-0716-0767-1_10DOI Listing
January 2020

Bound detergent molecules in bacterial reaction centers facilitate detection of tetryl explosive.

Photosynth Res 2020 Jul 6. Epub 2020 Jul 6.

Department of Physics, Concordia University, 7141 Sherbrooke Street West, Montreal, QC, H4B 1R6, Canada.

Bacterial reaction centers (BRC) from Rhodobacter sphaeroides were found to accelerate, about 100-fold, the reaction between tetryl (2,4,6-trinitrophenylmethylnitramine) explosive and n-lauryl-N-N-dimethylamine-N-oxide (LDAO) that results in the formation of picric acid-like product with characteristic UV-VIS absorption spectrum with peaks at 345 and 415 nm. Moreover, this product also affects the spectra of BRC cofactors in the NIR spectral region and stabilizes the conformational changes associated with slow charge recombination. The evolution of the NIR absorption changes correlated with the kinetics of the product formation. Read More

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http://dx.doi.org/10.1007/s11120-020-00770-7DOI Listing

Cell-free biosensors for rapid detection of water contaminants.

Nat Biotechnol 2020 Jul 6. Epub 2020 Jul 6.

Department of Chemical and Biological Engineering, Northwestern University, Evanston, IL, USA.

Lack of access to safe drinking water is a global problem, and methods to reliably and easily detect contaminants could be transformative. We report the development of a cell-free in vitro transcription system that uses RNA Output Sensors Activated by Ligand Induction (ROSALIND) to detect contaminants in water. A combination of highly processive RNA polymerases, allosteric protein transcription factors and synthetic DNA transcription templates regulates the synthesis of a fluorescence-activating RNA aptamer. Read More

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http://dx.doi.org/10.1038/s41587-020-0571-7DOI Listing

Electrochemical Biosensors Employing Natural and Artificial Heme Peroxidases on Semiconductors.

Sensors (Basel) 2020 Jul 1;20(13). Epub 2020 Jul 1.

Institute of Biochemistry and Biology, University of Potsdam, 14476 Potsdam, Germany.

Heme peroxidases are widely used as biological recognition elements in electrochemical biosensors for hydrogen peroxide and phenolic compounds. Various nature-derived and fully synthetic heme peroxidase mimics have been designed and their potential for replacing the natural enzymes in biosensors has been investigated. The use of semiconducting materials as transducers can thereby offer new opportunities with respect to catalyst immobilization, reaction stimulation, or read-out. Read More

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http://dx.doi.org/10.3390/s20133692DOI Listing

Graphene-Based Biosensor for Early Detection of Iron Deficiency.

Sensors (Basel) 2020 Jul 1;20(13). Epub 2020 Jul 1.

Electrical and Information Engineering Department, Covenant University, Ota 112233, Nigeria.

Iron deficiency (ID) is the most prevalent and severe nutritional disorder globally and is the leading cause of iron deficiency anemia (IDA). IDA often progresses subtly symptomatic in children, whereas prolonged deficiency may permanently impair development. Early detection and frequent screening are, therefore, essential to avoid the consequences of IDA. Read More

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http://dx.doi.org/10.3390/s20133688DOI Listing

Assessment of Gold Bio-Functionalization for Wide-Interface Biosensing Platforms.

Sensors (Basel) 2020 Jun 30;20(13). Epub 2020 Jun 30.

The Faculty of Science and Engineering, Åbo Akademi University, FI-20500 Turku, Finland.

The continuous improvement of the technical potential of bioelectronic devices for biosensing applications will provide clinicians with a reliable tool for biomarker quantification down to the single molecule. Eventually, physicians will be able to identify the very moment at which the illness state begins, with a terrific impact on the quality of life along with a reduction of health care expenses. However, in clinical practice, to gather enough information to formulate a diagnosis, multiple biomarkers are normally quantified from the same biological sample simultaneously. Read More

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http://dx.doi.org/10.3390/s20133678DOI Listing

Analysis and Testing of a Suitable Compatible Electrode's Material for Continuous Measurement of Glucose Concentration.

Sensors (Basel) 2020 Jun 30;20(13). Epub 2020 Jun 30.

Department of Cybernetic and Biomedical Engineering, VŠB-Technical University of Ostrava, 17, listopadu 2172/15, 708 00 Ostrava-Poruba, Czech Republic.

The subject of the submitted work is the proposal of electrodes for the continual measurement of the glucose concentration for the purpose of specifying further hemodynamic parameters. The proposal includes the design of the electronic measuring system, the construction of the electrodes themselves and the functionality of the entire system, verified experimentally using various electrode materials. The proposed circuit works on the basis of micro-ammeter measuring the size of the flowing electric current and the electrochemical measurement method is used for specifying the glucose concentration. Read More

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http://dx.doi.org/10.3390/s20133666DOI Listing

Microfluidic Technology for Nucleic Acid Aptamer Evolution and Application.

Adv Biosyst 2019 May 4;3(5):e1900012. Epub 2019 Apr 4.

School of Biomedical Sciences, LKS Faculty of Medicine, The University of Hong Kong, Hong Kong (SAR), China.

The intersection of microfluidics and aptamer technologies holds particular promise for rapid progress in a plethora of applications across biomedical science and other areas. Here, the influence of microfluidics on the field of aptamers, from traditional capillary electrophoresis approaches through innovative modern-day approaches using micromagnetic beads and emulsion droplets, is reviewed. Miniaturizing aptamer-based bioassays through microfluidics has the potential to transform diagnostics and embedded biosensing in the coming years. Read More

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http://dx.doi.org/10.1002/adbi.201900012DOI Listing

Development of an automated multienzymatic biosensor for risk assessment of pesticide contamination in water and food.

EFSA J 2018 Aug 27;16(Suppl 1):e16084. Epub 2018 Aug 27.

The goal of this research is to better address the problems related to the widespread presence of pesticides in the environment. Despite the unquestionable utility of the pesticides against various pests in the agricultural field, most pesticides and the corresponding pesticide residues are toxic to the environment and hazardous to human health. The recent literature on organophosphate compounds emphasises a clear correlation between their use and the occurrence of disorders in the nervous system, especially in children. Read More

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http://dx.doi.org/10.2903/j.efsa.2018.e16084DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7015495PMC

Covalent immobilization of acetylcholinesterase on a novel polyacrylic acid-based nanofiber membrane.

Eng Life Sci 2018 Apr 29;18(4):254-262. Epub 2018 Jan 29.

Department of Chemistry Faculty of Arts and Sciences Marmara University Istanbul Turkey.

In this study, polyacrylic acid-based nanofiber (NF) membrane was prepared via electrospinning method. Acetylcholinesterase (AChE) from was covalently immobilized onto polyacrylic acid-based NF membrane by demonstrating efficient enzyme immobilization, and immobilization capacity of polymer membranes was found to be 0.4 mg/g. Read More

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http://dx.doi.org/10.1002/elsc.201700130DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6999204PMC

Experimental characterization and simulation of amino acid and peptide interactions with inorganic materials.

Eng Life Sci 2018 Feb 26;18(2):84-100. Epub 2017 Jul 26.

Bioseparation Engineering Group Technical University of Munich München Germany.

Inspired by nature, many applications and new materials benefit from the interplay of inorganic materials and biomolecules. A fundamental understanding of complex organic-inorganic interactions would improve the controlled production of nanomaterials and biosensors to the development of biocompatible implants for the human body. Although widely exploited in applications, the interaction of amino acids and peptides with most inorganic surfaces is not fully understood. Read More

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http://dx.doi.org/10.1002/elsc.201700019DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6999452PMC
February 2018

Progress in enzyme inhibition based detection of pesticides.

Eng Life Sci 2018 Jan 2;18(1):4-19. Epub 2017 Aug 2.

Department of Biotechnology University of Food Technologies Plovdiv Bulgaria.

The previous few decades have seen the development of biosensors and their use in monitoring of pesticides in food and environmental samples. Although inhibition-based biosensors have been subject of several recent research works, their performance characteristics greatly depend on the type of immobilization and the presence of interfering compounds in the samples. Moreover, sensitivity, detection limits, and rapidity of the response are few of the other major features that need to be investigated further if they are to become operationally user-friendly. Read More

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http://dx.doi.org/10.1002/elsc.201700028DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6999348PMC
January 2018

Ratiometric fluorescent l-arginine and l-asparagine biosensors based on the oxazine 170 perchlorate-ethyl cellulose membrane.

Eng Life Sci 2017 Aug 12;17(8):847-856. Epub 2017 Jun 12.

School of Chemical Engineering Chonnam National University Gwangju Republic of Korea.

Ratiometric fluorescent l-arginine (Arg) and l-asparagine (Asn) biosensors were developed using an oxazine 170 perchlorate (O17) ethyl cellulose (EC) membrane and the enzymes entrapped into the matrix of EC and hydrogel polyurethane. The sensing principles were based on the hydrolysis reactions of urea and l-Arg under the catalysis of the urease and arginase to produce ammonia in the case of an l-Arg-sensing membrane and also on the hydrolysis reaction of l-Asn under the catalysis of asparaginase in the case of an l-Asn-sensing membrane. The O17-EC membrane reacted with the ammonia produced from the hydrolysis reactions and changed the fluorescence intensities at = 565 and 625 nm. Read More

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http://dx.doi.org/10.1002/elsc.201700033DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6999337PMC

Extended Metabolic Biosensor Design for Dynamic Pathway Regulation of Cell Factories.

iScience 2020 Jun 23;23(7):101305. Epub 2020 Jun 23.

Synthetic Biology and Biosystems Control Lab, I.U. de Automática e Informática Industrial (ai2), Universitat Politècnica de València, Camí de Vera S/N, 46022 Valencia, Spain. Electronic address:

Transcription factor-based biosensors naturally occur in metabolic pathways to maintain cell growth and to provide a robust response to environmental fluctuations. Extended metabolic biosensors, i.e. Read More

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http://dx.doi.org/10.1016/j.isci.2020.101305DOI Listing

Enlightening the advancements in electrochemical bioanalysis for the diagnosis of Alzheimer's disease and other neurodegenerative disorders.

J Pharm Biomed Anal 2020 Jun 21;189:113437. Epub 2020 Jun 21.

Department of Analytical Chemistry, Faculty of Chemical Sciences, Complutense University of Madrid, Madrid, 28040, Spain. Electronic address:

Neurodegenerative disorders (NDD), and particularly Alzheimer's disease (AD), are one of the greatest challenges facing our current medicine and society because of its increasing incidence and the high burden imposed both on patients' families and health systems. Despite this, their accurate diagnosis, mostly conducted by cerebrospinal fluid (CSF) analysis or neuroimaging techniques, costly, time-consuming, and unaffordable for most of the population, remains a complex task. In this situation, electrochemical biosensors are flourishing as promising alternative tools for the simple, fast, and low-cost diagnosis of NDD/AD. Read More

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http://dx.doi.org/10.1016/j.jpba.2020.113437DOI Listing

Time-resolved fluorescence spectroscopy based evaluation of stability of glucose oxidase.

Int J Biol Macromol 2020 Jul 3. Epub 2020 Jul 3.

Department of Physical Chemistry, Institute of Chemistry, Faculty of Chemistry and Geosciences, Vilnius University, Naugarduko Str. 24, LT-03225 Vilnius, Lithuania; Center for Physical Sciences and Technology, Sauletekio Av. 3, LT-10257 Vilnius, Lithuania. Electronic address:

Glucose oxidase (GOx) is one of the most frequently used enzymes in a design of enzymatic biosensors and biofuel cells, which are novel electrical energy generation systems. Therefore, a better understanding of the mode of action of this enzyme is very important for further development of GOx-based sensors. In this research fluorescence properties of GOx in different acidic media have been estimated by the evaluation of redox states of active center that is flavine adenine dinucleotide (FAD). Read More

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http://dx.doi.org/10.1016/j.ijbiomac.2020.06.284DOI Listing

Investigating the effect of antibody-antigen reactions on the internal convection in a sessile droplet via micro particle image velocimetry and DLVO analysis.

Langmuir 2020 Jul 6. Epub 2020 Jul 6.

Evaporation of antigen laden sessile droplets on antibody immobilized PDMS substrates could be used in place of microwells for detection purposes owing to lesser requirement of analyte and reduced reaction time. To develop such techniques, the effects of different parameters on the reaction efficiency and on the resulting deposition patterns of antigens on the surface after evaporation need to well understood. While the resultant deposition patterns from evaporation of droplets of biological fluids on surfaces are being studied for various biomedical applications, systems where the analyte of interest in the droplet binds to the surface have not been investigated till date. Read More

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http://dx.doi.org/10.1021/acs.langmuir.0c01162DOI Listing

Manganese Oxide Nanoparticle Synthesis by Thermal Decomposition of Manganese(II) Acetylacetonate.

J Vis Exp 2020 Jun 18(160). Epub 2020 Jun 18.

Department of Chemical and Biomedical Engineering, West Virginia University;

For biomedical applications, metal oxide nanoparticles such as iron oxide and manganese oxide (MnO), have been used as biosensors and contrast agents in magnetic resonance imaging (MRI). While iron oxide nanoparticles provide constant negative contrast on MRI over typical experimental timeframes, MnO generates switchable positive contrast on MRI through dissolution of MnO to Mn at low pH within cell endosomes to 'turn ON' MRI contrast. This protocol describes a one-pot synthesis of MnO nanoparticles formed by thermal decomposition of manganese(II) acetylacetonate in oleylamine and dibenzyl ether. Read More

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http://dx.doi.org/10.3791/61572DOI Listing

Assessment of Cellular Oxidation using a Subcellular Compartment-Specific Redox-Sensitive Green Fluorescent Protein.

J Vis Exp 2020 Jun 18(160). Epub 2020 Jun 18.

Division of Radiation Health, Pharmaceutical Sciences, University of Arkansas for Medical Sciences;

Measuring the intracellular oxidation/reduction balance provides an overview of the physiological and/or pathophysiological redox status of an organism. Thiols are especially important for illuminating the redox status of cells via their reduced dithiol and oxidized disulfide ratios. Engineered cysteine-containing fluorescent proteins open a new era for redox-sensitive biosensors. Read More

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http://dx.doi.org/10.3791/61229DOI Listing

Nanoparticle-based biosensors for detection of extracellular vesicles in liquid biopsies.

J Mater Chem B 2020 Jul 6. Epub 2020 Jul 6.

Aragón Materials Science Institute (ICMA), CSIC/University of Zaragoza, Zaragoza, Spain. and Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Spain.

Tumor-derived extracellular vesicles have emerged as an alternative source of cancer biomarkers in liquid biopsies. Despite their clinical potential, traditional methods for isolation and analysis have hampered their translation into the clinic. The use of nanomaterial-based biosensors can speed up the development of analytical methods for quantifying extracellular vesicles in a specific, highly reproducible, robust, fast and inexpensive way. Read More

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http://dx.doi.org/10.1039/d0tb00861cDOI Listing

Genome-wide gene-deletion screening identifies mutations that significantly enhance explosives vapor detection by a microbial sensor.

N Biotechnol 2020 Jul 2. Epub 2020 Jul 2.

Department of Plant and Environmental Sciences, Institute of Life Sciences, The Hebrew University of Jerusalem, Israel. Electronic address:

Genetically engineered microbial biosensors, capable of detecting traces of explosives residues above buried military ordnance and emitting an optical signal in response, may potentially serve for the standoff detection of buried landmines. A promising candidate for such an application is a previously reported Escherichia coli-based reporter strain that employs the yqjF gene promoter as its sensing element; however, for this sensor to be able to detect actual landmines reliably, it was necessary for its detection sensitivity and signal intensity to be enhanced. In this study, a high-throughput approach was employed to screen the effects of individual gene deletions on yqjF activation by 2,4-dinitrotoluene (DNT). Read More

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http://dx.doi.org/10.1016/j.nbt.2020.06.002DOI Listing

Nanozymes in electrochemical affinity biosensing.

Mikrochim Acta 2020 Jul 3;187(8):423. Epub 2020 Jul 3.

Departamento de Química Analítica, Facultad de CC. Químicas, Universidad Complutense de Madrid, E-28040, Madrid, Spain.

Over the past decade, artificial nanomaterials that exhibit properties similar to those of enzymes are gaining attraction in electrochemical biosensing as highly stable and low-cost alternatives to enzymes. This review article discusses the main features of the various nanomaterials (metal oxide, metal, and carbon-based materials) explored so far to mimic different kinds of enzymes. The unprecedented opportunities imparted by these functional nanomaterials or their nanohybrids, mostly providing peroxidase-like activity, in electrochemical affinity biosensing are critically discussed mainly in connection with their use as catalytic labels or electrode surface modifiers by highlighting representative strategies reported in the past 5 years with application in the food, environmental, and biomedical fields. Read More

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http://dx.doi.org/10.1007/s00604-020-04390-9DOI Listing

Development of fluorescence oligonucleotide probes based on cytosine- and guanine-rich sequences.

Sci Rep 2020 Jul 3;10(1):11006. Epub 2020 Jul 3.

Faculty of Chemistry, Adam Mickiewicz University, Uniwersytetu Poznanskiego 8, 61-614, Poznan, Poland.

The properties of cytosine- and guanine-rich oligonucleotides contributed to employing them as sensing elements in various biosensors. In this paper, we report our current development of fluorescence oligonucleotide probes based on i-motif or G-quadruplex forming oligonucleotides for cellular measurements or bioimaging applications. Additionally, we also focus on the spectral properties of the new fluorescent silver nanoclusters based system (ChONC12-AgNCs) that is able to anchor at the Langmuir monolayer interface, which is mimicking the surface of living cells membrane. Read More

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http://dx.doi.org/10.1038/s41598-020-67745-5DOI Listing

Genetically encoded biosensors for the detection of rapamycin: toward the screening of agonists and antagonists.

Analyst 2020 Jul 3. Epub 2020 Jul 3.

Department of Biomedical Engineering (BK21 plus), Dongguk University, Seoul 04620, Korea.

Biosensors are valuable tools for the rapid screening of biological targets with high sensitivity and specificity. It is important to screen biological events in their native context for pharmacological and toxicological applications. However, in vitro biosensors often require purified probes and targets for screening, thus providing limited information on the biological activities of targets in their native environment. Read More

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http://dx.doi.org/10.1039/d0an01116aDOI Listing

A Label-Free and Regenerable Aptasensor for Real-Time Detection of Cadmium (II) by Dual Polarization Interferometry.

Anal Chem 2020 Jul 3. Epub 2020 Jul 3.

Recently, numerous aptamer-based biosensors have been developed to detect heavy metal ions. Most of aptamer-based biosensors only can be used to get some quantitative information. The mechanism of the interaction between aptamer and metal ions, however, is rarely studied. Read More

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http://dx.doi.org/10.1021/acs.analchem.0c01710DOI Listing

Ultrasonic atomizer based development of pH sensor for real time analysis.

Sci Rep 2020 Jul 2;10(1):10910. Epub 2020 Jul 2.

Room No. SB-216, Discipline of Biosciences and Biomedical Engineering, Indian Institute of Technology Indore, Khandwa Road, Simrol, Indore, Madhya Pradesh, 453552, India.

Fluorescent pH biosensors have gained importance owing to their low cost utilization in real time monitoring of biological and food samples in comparison to conventional pH meters. The research reports a novel method of ultrasonic atomization for developing a fluorescent pH sensor for real-time analysis made of Fluorescein isothiocyanate (FITC)-dextran/FITC-dextran-Tris (2, 2'-bipyridyl) dichlororuthenium (II) hexahydrate as indicator and reference fluorophores, respectively. The process of ultrasonic atomization ensures formation of monodisperse dye immobilized alginate microspheres ensuring efficient pH sensing. Read More

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http://dx.doi.org/10.1038/s41598-020-68005-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7331635PMC

Dual plasmonic nanostructures for switching polarity of hot electron-induced photocurrent.

Nanoscale 2020 Jul 2. Epub 2020 Jul 2.

School of Physics and National Laboratory of Solid State Microstructures, Collaborative Innovation Center of Advanced Microstructures, Nanjing University, Nanjing 210093, China.

We report on the experimental investigation of polarity-switchable hot electron-induced photocurrents in dual-plasmonic nanostructures, consisting of two layers of gold nanoparticles (AuNPs) separated by a TiO2 film. Hot electrons generated through the non-radiative decay of the localized surface plasmon resonances supported by the top and bottom layers of AuNPs can be simultaneously injected into the TiO2 film in opposite directions and counteract each other. As a result, the polarity and magnitude of the net photocurrents can be tailored by controlling the population of hot electrons either generated from or collected by the two layers of AuNPs. Read More

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http://dx.doi.org/10.1039/c9nr10413eDOI Listing

Enzyme-based optical biosensors for organophosphate class of pesticide detection.

Phys Chem Chem Phys 2020 Jul 2. Epub 2020 Jul 2.

Radiation & Photochemistry Division, Bhabha Atomic Research Centre, Mumbai 400 085, India. and Homi Bhabha National Institute, Training School Campus, Anushaktinagar, Mumbai 400 094, India.

Organophosphorus pesticides, widely used as insecticides for crop protection, are classified as an extremely toxic class of chemical compounds by the World Health Organization (WHO). Organophosphorus pesticides show marked specificity for the enzyme acetylcholinesterase, and can cause irreversible harm to the nervous system. The excessive use of organophosphorus pesticides, specifically in developing countries, made WHO to impose a ban on certain organophosphorus pesticides being sold to the developing nations. Read More

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http://dx.doi.org/10.1039/d0cp01647kDOI Listing

A hybrid system integrating xylose dehydrogenase and NAD coupled with PtNPs@MWCNTs composite for the real-time biosensing of xylose.

Analyst 2020 Jul 2. Epub 2020 Jul 2.

Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 32 West Seventh Avenue, Tianjin Airport Economic Area, Tianjin, 300308, P. R. China. and School of Chemical Engineering, University of Chinese Academy of Sciences, 19A Yuquan Road, Shijingshan District, Beijing, 100049, P. R. China.

The wide application of xylose in the food, beverage, and pharmaceutical industries, as well as in the booming field of biorefinery, raises the demand for a rapid, accurate, and real-time xylose-sensing technique to rival the conventional methods based on chromatography, spectroscopy, and electrochemical analysis using non-specific enzymes or abiotic catalysts. Herein, a hybrid system comprising polyethylene glycerol swing-arm-tethered NAD+ and xylose dehydrogenase (XDH), coupled with platinum nanoparticles deposited on carbon nanotubes (PtNPs@MWCNTs), was constructed for the real-time sensing of xylose. The use of the PtNPs@MWCNTs composite enhanced the sensitivity of the electric response and reduced the oxidation potential of NADH significantly. Read More

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http://dx.doi.org/10.1039/d0an00880jDOI Listing

A Flexible 3D Organic Preamplifier for a Lactate Sensor.

Macromol Biosci 2020 Jul 1:e2000144. Epub 2020 Jul 1.

Department of Creative IT Engineering / Department of Chemical Engineering, Pohang University of Science and Technology (POSTECH), 77 Cheongam-Ro, Nam-Gu, Pohang, 37673, Republic of Korea.

Organic transistors are promising platforms for wearable biosensors. However, the strategies to improve signal amplification have yet to be determined, particularly regarding biosensors that generate very weak signals. In this study, an organic voltage amplifier is presented for a lactate sensor on flexible plastic foil. Read More

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http://dx.doi.org/10.1002/mabi.202000144DOI Listing

Simple experimental procedures to distinguish photothermal from hot-carrier processes in plasmonics.

Light Sci Appl 2020 28;9:108. Epub 2020 Jun 28.

ICFO-Institut de Ciències Fotòniques, The Barcelona Institute of Science and Technology, 08860 Castelldefels, Barcelona, Spain.

Light absorption and scattering of plasmonic metal nanoparticles can lead to non-equilibrium charge carriers, intense electromagnetic near-fields, and heat generation, with promising applications in a vast range of fields, from chemical and physical sensing to nanomedicine and photocatalysis for the sustainable production of fuels and chemicals. Disentangling the relative contribution of thermal and non-thermal contributions in plasmon-driven processes is, however, difficult. Nanoscale temperature measurements are technically challenging, and macroscale experiments are often characterized by collective heating effects, which tend to make the actual temperature increase unpredictable. Read More

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http://dx.doi.org/10.1038/s41377-020-00345-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7321931PMC

Application of Nanostructured Carbon-Based Electrochemical (Bio)Sensors for Screening of Emerging Pharmaceutical Pollutants in Waters and Aquatic Species: A Review.

Nanomaterials (Basel) 2020 Jun 29;10(7). Epub 2020 Jun 29.

REQUIMTE-LAQV, Instituto Superior de Engenharia do Porto, Instituto Politécnico do Porto, Rua Dr. António Bernardino de Almeida, 431, 4249-015 Porto, Portugal.

Pharmaceuticals, as a contaminant of emergent concern, are being released uncontrollably into the environment potentially causing hazardous effects to aquatic ecosystems and consequently to human health. In the absence of well-established monitoring programs, one can only imagine the full extent of this problem and so there is an urgent need for the development of extremely sensitive, portable, and low-cost devices to perform analysis. Carbon-based nanomaterials are the most used nanostructures in (bio)sensors construction attributed to their facile and well-characterized production methods, commercial availability, reduced cost, high chemical stability, and low toxicity. Read More

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http://dx.doi.org/10.3390/nano10071268DOI Listing

Challenges for Microelectronics in Non-Invasive Medical Diagnostics.

Sensors (Basel) 2020 Jun 29;20(13). Epub 2020 Jun 29.

Politecnico di Milano, Dipartimento di Elettronica Informazione e Bioingegneria, 20133 Milano, Italy.

Microelectronics is emerging, sometimes with changing fortunes, as a key enabling technology in diagnostics. This paper reviews some recent results and technical challenges which still need to be addressed in terms of the design of CMOS analog application specific integrated circuits (ASICs) and their integration in the surrounding systems, in order to consolidate this technological paradigm. Open issues are discussed from two, apparently distant but complementary, points of view: micro-analytical devices, combining microfluidics with affinity bio-sensing, and gamma cameras for simultaneous multi-modal imaging, namely scintigraphy and magnetic resonance imaging (MRI). Read More

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http://dx.doi.org/10.3390/s20133636DOI Listing

Technologies of time: women's practices of trying to conceive with ovulation biosensing.

Authors:
Joann Wilkinson

Sociol Health Illn 2020 Jul 1. Epub 2020 Jul 1.

Department of Educational Research, Lancaster University, Lancaster, UK.

Ovulation biosensors are devices worn on or used with the body, which can help women detect ovulation. The manufacturers of such devices claim that if women know when they ovulate, couples can arrange heterosexual intercourse during this time, and thus increase their chances of conceiving. Within the contemporary UK context, in which becoming pregnant is presented in the popular media, and in medical discourses, as more difficult for women in their thirties and forties, manufacturers' claims are attractive for those trying to conceive. Read More

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http://dx.doi.org/10.1111/1467-9566.13150DOI Listing

High-Sensitive Assay of Nucleic Acid Using Tetrahedral DNA Probes and DNA Concatamers with a Surface-Enhanced Raman Scattering/Surface Plasmon Resonance Dual-Mode Biosensor Based on a Silver Nanorod-Covered Silver Nanohole Array.

ACS Appl Mater Interfaces 2020 Jul 1. Epub 2020 Jul 1.

Key Laboratory for Organic Electronics and Information Displays & Jiangsu Key Laboratory for Biosensors, Institute of Advanced Materials (IAM), Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM), Nanjing University of Posts & Telecommunications, Nanjing 210023, China.

A novel surface-enhanced Raman scattering/surface plasmon resonance (SERS/SPR) dual-mode biosensor prepared on a silver nanorod-covered silver nanohole (Ag NR-NH) array by surface modification of tetrahedral DNA probes is proposed for highly sensitive detecting nucleic acids by a special signal amplification strategy of DNA supersandwich. The Ag NR-NH with a large area and uniformly arrayed nanostructure possesses excellent anisotropic extraordinary optical transmission and strong localized surface plasmon resonance, which lead to sensitive SPR response to the change of a local refractive index and strong localized electric fields for excellent SERS activity. To obtain high sensitivity and specificity, smart tetrahedral DNA probes are immobilized onto the Ag NR-NH array and the DNA supersandwich sensing strategy, including the signal amplification of DNA concatamers, is used. Read More

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http://dx.doi.org/10.1021/acsami.0c08453DOI Listing

Extending the Debye scattering equation for diffraction from a cylindrically averaged group of atoms: detecting molecular orientation at an interface.

Acta Crystallogr A Found Adv 2020 Jul 20;76(Pt 4):468-473. Epub 2020 May 20.

School of Physics A28, The University of Sydney, NSW 2006, Australia.

The Debye scattering equation is now over 100 years old and has been widely used to interpret diffraction patterns from randomly oriented groups of atoms. The present work develops and applies a related equation that calculates diffraction intensity from groups of atoms randomly oriented about a fixed axis, a scenario that occurs when molecules are oriented at an interface by the presentation of a binding motif as in antibody binding. Using an example biomolecule, the high level of sensitivity of the diffraction pattern to the orientation of the molecule and to the direction of the incident beam is shown. Read More

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http://dx.doi.org/10.1107/S2053273320005276DOI Listing

Efficient synthesis of amino acids capped gold nanoparticles from easily reducible aryldiazonium tetrachloroaurate(III) salts for cellular uptake study.

Amino Acids 2020 Jun 30. Epub 2020 Jun 30.

Center for Advanced Materials Research, Research Institute for Science and Engineering, University of Sharjah, 27272, Sharjah, United Arab Emirates.

Biomimetic synthesis of gold nanoparticles (GNPs) is critical in biomedical applications. Gold bioconjugates were fabricated by capping the water-dispersible gold-carbon nanoparticles with tyrosine, tryptophan and cysteine amino acids. Incubation of the water-soluble and easily reducible aryldiazonium gold(III) salt [HOOC-4-CHN≡N]AuCl with amino acids at room temperature formed a purple color over a few minutes with tryptophan and tyrosine and over two hours with cysteine. Read More

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http://dx.doi.org/10.1007/s00726-020-02862-zDOI Listing

Biofunctional Polymer Coated Au Nanoparticles Prepared via RAFT-Assisted Encapsulating Emulsion Polymerization and Click Chemistry.

Polymers (Basel) 2020 Jun 27;12(7). Epub 2020 Jun 27.

Department of Chemistry, CICECO-Aveiro Institute of Materials, University of Aveiro, 3810-193 Aveiro, Portugal.

The use of reversible addition-fragmentation chain transfer (RAFT)-assisted encapsulating emulsion polymerization (REEP) has been explored to prepare diverse types of colloidal stable core-shell nanostructures. A major field of application of such nanoparticles is in emergent nanomedicines, which require effective biofunctionalization strategies, in which their response to bioanalytes needs to be firstly assessed. Herein, functional core-shell nanostructures were prepared via REEP and click chemistry. Read More

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http://dx.doi.org/10.3390/polym12071442DOI Listing

Biosensors for D-Amino Acids: Detection Methods and Applications.

Int J Mol Sci 2020 Jun 27;21(13). Epub 2020 Jun 27.

Department of Biotechnology and Life Sciences, University of Insubria, via J.H. Dunant 3, 21100 Varese, Italy.

D-enantiomers of amino acids (D-AAs) are only present in low amounts in nature, frequently at trace levels, and for this reason, their biological function was undervalued for a long time. In the past 25 years, the improvements in analytical methods, such as gas chromatography, HPLC, and capillary electrophoresis, allowed to detect D-AAs in foodstuffs and biological samples and to attribute them specific biological functions in mammals. These methods are time-consuming, expensive, and not suitable for online application; however, life science investigations and industrial applications require rapid and selective determination of D-AAs, as only biosensors can offer. Read More

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http://dx.doi.org/10.3390/ijms21134574DOI Listing
June 2020
2.862 Impact Factor

Biofabrication offers future hope for tackling various obstacles and challenges in tissue engineering and regenerative medicine: A Perspective.

Int J Bioprint 2019 31;5(1):153. Epub 2018 Dec 31.

Graduate School of Science and Engineering for Research (Engineering), University of Toyama, Toyama 930-8555, Japan.

Biofabrication is an emerging multidisciplinary field that makes a revolutionary impact on the researches on life science, biomedical engineering, and both basic and clinical medicine, has progressed tremendously over the past few years. Recently, there has been a big boom in three-dimensional (3D) printing or additive manufacturing (AM) research worldwide, and there is a significant increase not only in the number of researchers turning their attention to AM but also publications demonstrating the potential applications of 3D printing techniques in multiple fields. Biofabrication and bioprinting hold great promise for the innovation of engineering-based organ replacing medicine. Read More

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http://dx.doi.org/10.18063/ijb.v5i1.153DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7294687PMC
December 2018

Biosensors for detection of Tau protein as an Alzheimer's disease marker.

Int J Biol Macromol 2020 Jun 27;162:1100-1108. Epub 2020 Jun 27.

Research Center for Biochemistry and Nutrition in Metabolic Diseases, Institute for Basic Sciences, Kashan University of Medical Sciences, Kashan, Iran. Electronic address:

Known as a main neural MAP (microtubule associated protein), tau protein contributes to stabilizing microtubules involved in cellular transmission. Tau dysfunction is mainly associated with neurodegenerative diseases, particularly Alzheimer's disease (AD). In these patients, all the six tau isoforms, which are in hyperphosphorylated form, are first aggregated and then polymerized into neurofibrillary tangles inside the brain. Read More

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http://dx.doi.org/10.1016/j.ijbiomac.2020.06.239DOI Listing

New Approach for Making Standard the Development of Biosensing Devices by a Modular Multi-Purpose Design.

IEEE Trans Nanobioscience 2020 Jul;19(3):339-346

The fast widening of biosensing applications, such as healthcare, drug delivery, food, and military industries, is increasing the need for generality and compatibility among different sensors. To address this challenge, we present here an innovative approach for the fast development of new electronic biosensing systems, linking a custom-designed front-end with a multi-purpose system. We envision an open tool to help designers to focus on the target molecule and related detection method instead of designing each time a dedicated electronic device. Read More

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http://dx.doi.org/10.1109/TNB.2020.2995230DOI Listing

Functional nucleic acid-based fluorescence polarization/anisotropy biosensors for detection of biomarkers.

Anal Bioanal Chem 2020 Jun 29. Epub 2020 Jun 29.

Research Center for Bioengineering and Sensing Technology, School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing, 100083, China.

The sensitive and selective detection of biomarkers plays a crucial role in disease diagnostics, drug discovery, and early screening of cancers. The achievement of this goal highly depends on the continuous development of biosensing technologies. Among them, fluorescence anisotropy/polarization (FA/FP) analysis receives increasing interest due to the advantage of simple operation, fast response, and no background interference. Read More

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http://dx.doi.org/10.1007/s00216-020-02754-xDOI Listing

Real-time monitoring of peroxiredoxin oligomerization dynamics in living cells.

Proc Natl Acad Sci U S A 2020 Jun 29. Epub 2020 Jun 29.

Division of Redox Regulation, DKFZ-ZMBH Alliance, German Cancer Research Center (DKFZ), 69120 Heidelberg, Germany;

Peroxiredoxins are central to cellular redox homeostasis and signaling. They serve as peroxide scavengers, sensors, signal transducers, and chaperones, depending on conditions and context. Typical 2-Cys peroxiredoxins are known to switch between different oligomeric states, depending on redox state, pH, posttranslational modifications, and other factors. Read More

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http://dx.doi.org/10.1073/pnas.1915275117DOI Listing

Immunosensors containing solution blow spun fibers of poly(lactic acid) to detect p53 biomarker.

Mater Sci Eng C Mater Biol Appl 2020 Oct 27;115:111120. Epub 2020 May 27.

São Carlos Institute of Physics, University of São Paulo, 13566-590 São Carlos, Brazil. Electronic address:

This paper reports on biosensors made with a matrix of polylactic acid (PLA) fibers, which are suitable for immobilization of the anti-p53 active layer for detection of p53 biomarker. The PLA fibers were produced with solution blow spinning, a method that is advantageous for its simplicity and possibility to tune the fiber properties. For the biosensors, the optimized time to deposit the fibers was 60 s, with which detection of p53 could be achieved with the limit of detection of 11 pg/mL using electrical impedance spectroscopy. Read More

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http://dx.doi.org/10.1016/j.msec.2020.111120DOI Listing
October 2020

Osteoblastic exosomes. A non-destructive quantitative approach of alkaline phosphatase to assess osteoconductive nanomaterials.

Mater Sci Eng C Mater Biol Appl 2020 Oct 13;115:110931. Epub 2020 Apr 13.

Grup de Sensors i Biosensors, Departament de Química, Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain.

Alkaline phosphatase (ALP) is an essential biomarker of osteoblastic activity. Currently, ALP activity has been used to study bone mineralization mechanisms and osteoactive biomaterials among others. The ALP quantification is usually performed by destructive methods either on growing cells or cells lysate in which the osteoconductive biomaterial is being assessed. Read More

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http://dx.doi.org/10.1016/j.msec.2020.110931DOI Listing
October 2020