57,465 results match your criteria Biosensors & bioelectronics[Journal]


Early stage detection and screening of ovarian cancer: A research opportunity and significant challenge for biosensor technology.

Biosens Bioelectron 2019 Mar 20;135:71-81. Epub 2019 Mar 20.

Department of Chemistry, University of Toronto, 80 St. George Street, Toronto, Ontario, Canada M5S 3H6. Electronic address:

Women diagnosed with late-stage ovarian cancer suffer a very high rate of mortality. Accordingly, it is imperative to detect and diagnose the disease as early as possible in its development. Achievement of this aim implies relatively large scale screening of women at an age of clinically significance through assay of biomarkers for disease present in blood or serum. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S09565663193024
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http://dx.doi.org/10.1016/j.bios.2019.03.041DOI Listing
March 2019
1 Read

The Least Mating Pathway: Synthetically Refactoring a Familiar Signaling System for New Applications.

Cell 2019 Apr;177(3):521-523

Department of Bioengineering, Rice University, Houston, TX 77030, USA; Department of Biosciences, Rice University, Houston, TX 77030, USA. Electronic address:

Synthetic refactoring makes naturally occurring regulatory systems more amenable to manipulation by removing or recoding their natural genetic complexity. Shaw et al. apply this technique to the yeast mating response pathway, creating a simplified, highly engineerable signaling module that can be used to construct precisely optimized, application-specific GPCR biosensors. Read More

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http://dx.doi.org/10.1016/j.cell.2019.04.003DOI Listing

Dishevelled-3 conformation dynamics analyzed by FRET-based biosensors reveals a key role of casein kinase 1.

Nat Commun 2019 Apr 18;10(1):1804. Epub 2019 Apr 18.

Department of Experimental Biology, Faculty of Science, Masaryk University, Brno, 62500, Czech Republic.

Dishevelled (DVL) is the key component of the Wnt signaling pathway. Currently, DVL conformational dynamics under native conditions is unknown. To overcome this limitation, we develop the Fluorescein Arsenical Hairpin Binder- (FlAsH-) based FRET in vivo approach to study DVL conformation in living cells. Read More

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http://dx.doi.org/10.1038/s41467-019-09651-7DOI Listing

A Review on Surface-Enhanced Raman Scattering.

Biosensors (Basel) 2019 Apr 17;9(2). Epub 2019 Apr 17.

Department of Materials Science and Engineering, Rutgers University, 607 Taylor Road, Piscataway, NJ 08854, USA.

Surface-enhanced Raman scattering (SERS) has become a powerful tool in chemical, material and life sciences, owing to its intrinsic features (i.e., fingerprint recognition capabilities and high sensitivity) and to the technological advancements that have lowered the cost of the instruments and improved their sensitivity and user-friendliness. Read More

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http://dx.doi.org/10.3390/bios9020057DOI Listing

Streamwise-travelling viscous waves in channel flows.

J Eng Math 2018 23;111(1):23-49. Epub 2018 Feb 23.

2School of Engineering, Fraser Noble Building, King's College, University of Aberdeen, Aberdeen, AB24 3UE UK.

The unsteady viscous flow induced by streamwise-travelling waves of spanwise wall velocity in an incompressible laminar channel flow is investigated. Wall waves belonging to this category have found important practical applications, such as microfluidic flow manipulation via electro-osmosis and surface acoustic forcing and reduction of wall friction in turbulent wall-bounded flows. An analytical solution composed of the classical streamwise Poiseuille flow and a spanwise velocity profile described by the parabolic cylinder function is found. Read More

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http://dx.doi.org/10.1007/s10665-018-9953-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6434986PMC
February 2018

Sampling and multiplexing in lab-on-paper bioelectroanalytical devices for glucose determination.

Biosens Bioelectron 2019 Apr 5;135:64-70. Epub 2019 Apr 5.

Departamento de Química Física y Analítica, Facultad de Química, Universidad de Oviedo, 33006, Oviedo, Spain. Electronic address:

In this work, we present a multiplexed (eight simultaneous measurements) paper-based electrochemical device developed in a very simple way and using low-cost materials, such as paper, carbon ink and multifunctional connector headers. Meanwhile, we have also combined the paper-based electrochemical platform with a glass-fiber strip in order to integrate easily a sampling step. Both approaches, simultaneous measuring and sampling, have been applied to the determination of glucose using bienzymatic biosensors. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S09565663193028
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http://dx.doi.org/10.1016/j.bios.2019.04.006DOI Listing
April 2019
1 Read

Sensors that Learn: The Evolution from Taste Fingerprints to Patterns of Early Disease Detection.

Micromachines (Basel) 2019 Apr 16;10(4). Epub 2019 Apr 16.

Department of Biomaterials, College of Dentistry, Bioengineering Institute, New York University, New York, NY 10010, USA.

The McDevitt group has sustained efforts to develop a programmable sensing platform that offers advanced, multiplexed/multiclass chem-/bio-detection capabilities. This scalable chip-based platform has been optimized to service real-world biological specimens and validated for analytical performance. Fashioned as a sensor that learns, the platform can host new content for the application at hand. Read More

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http://dx.doi.org/10.3390/mi10040251DOI Listing

Optical multiplexed bioassays improve biomedical diagnostics.

Angew Chem Int Ed Engl 2019 Apr 17. Epub 2019 Apr 17.

CHINA.

Conventional analytical methods based on single detection to determine one disease marker are usually not sufficient and accurate because the progression of disease generally involves multiple chemicals and biomolecules. The drive for simultaneous analysis of multiple targets, which plays a key role in both basic biomedical research and clinical applications, demands the development of multiplexed bioassays with high-throughput. In this minireview, we systematically summarize the recent progress of optical multiplexed analytical techniques to improve biomedical diagnostics, in which fluorescence and surface enhanced Raman scattering (SERS) with distinctive optical features are chosen as the main readout signals. Read More

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http://dx.doi.org/10.1002/anie.201901964DOI Listing

Membrane-permeant, environment-sensitive dyes generate biosensors within living cells.

J Am Chem Soc 2019 Apr 16. Epub 2019 Apr 16.

Dyes with environment-sensitive fluorescence have proven useful to study the spatio-temporal dynamics of protein activity in living cells. When attached to proteins, their fluorescence can reflect protein conformational changes, posttranslational modifications or protein interactions. However, the utility of such dye-protein conjugates has been limited because it is difficult to load them into cells. Read More

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http://pubs.acs.org/doi/10.1021/jacs.8b09841
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http://dx.doi.org/10.1021/jacs.8b09841DOI Listing
April 2019
2 Reads

The Interference Effect of Silica Colloidal Crystal Films and Their Applications on Biosensing.

Anal Chem 2019 Apr 17. Epub 2019 Apr 17.

With the aim to develop better and reliable interference effective substrates, silica colloidal crystal films with different sphere di-ameters and film thicknesses were successfully made by an improved vertical deposition method and a systematic investigation of their reflectometric interference spectroscopy (RIfS) properties was presented in this work. The influence of silica sphere diameter and film thickness on the RIfS signals was studied. Results showed that the film thickness is the key factor of RIfS signals. Read More

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http://pubs.acs.org/doi/10.1021/acs.analchem.9b00620
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http://dx.doi.org/10.1021/acs.analchem.9b00620DOI Listing
April 2019
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Design and demonstration of tunable amplified sensitivity of AlGaN/GaN high electron mobility transistor (HEMT)-based biosensors in human serum.

Anal Chem 2019 Apr 17. Epub 2019 Apr 17.

We have developed a swift and simplistic protein immunoassay using aptamer functionalized AlGaN/GaN high electron mobility transistors (HEMTs). The unique design of the sensor facilitates protein detection in physiological salt environment overcoming charge screening effects, without requiring sample pre-processing. This study reports a tunable and amplified sensitivity of solution-gated electric double layer (EDL) HEMT-based biosensors, which demonstrates significantly enhanced sensitivity by designing a smaller gap between the gate electrode and the detection, and by operating at higher gate voltage. Read More

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http://dx.doi.org/10.1021/acs.analchem.9b00353DOI Listing

Perylene-Based Photoactive Material as Double-Strand DNA Intercalating Probe for Ultrasensitive Photoelectrochemical Biosensing.

ACS Appl Mater Interfaces 2019 Apr 17. Epub 2019 Apr 17.

Photoelectrochemical (PEC) sensing techniques have attracted considerable concerns because of the intrinsic merit of complete separation between the excitation light and responsive current, but still remain a great challenge for further potential application. It is assigned to the scarcity of photoactive materials with narrow band gap/good biosafety/high photon-to-electron conversion efficiency, and unfavorable processing methods for photoactive materials on ITO. Herein, we employed perylene-based polymer (PTC-NH) with exceptional photoelectrical properties to develop a red-light driven PEC sensor for ultrasensitive biosensing based on its superior electrostatic intercalation efficiency in dsDNA to that in ssDNA, with DNA adenine methyltransferase (Dam MTase) as the model target. Read More

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http://dx.doi.org/10.1021/acsami.9b04299DOI Listing

A Machine Learning-based Approach for Collaborative Non-Adherence Detection during Opioid Abuse Surveillance using a Wearable Biosensor.

Biomed Eng Syst Technol Int Jt Conf BIOSTEC Revis Sel Pap 2019 Feb;5:310-318

Worcester Polytechnic Institute, Worcester, MA, U.S.A.

Wearable biosensors can be used to monitor opioid use, a problem of dire societal consequence given the current opioid epidemic in the US. Such surveillance can prompt interventions that promote behavioral change. The effectiveness of biosensor-based monitoring is threatened by the potential of a patient's to the monitoring. Read More

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http://dx.doi.org/10.5220/0007382503100318DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6461698PMC
February 2019

Integrated Printed Microfluidic Biosensors.

Trends Biotechnol 2019 Apr 13. Epub 2019 Apr 13.

Biosensors and Bioelectronics Centre, Department of Physics, Chemistry and Biology (IFM), Linköping University, 58183, Linköping, Sweden. Electronic address:

Integrated printed microfluidic biosensors are one of the most recent point-of-care (POC) sensor developments. Fast turnaround time for production and ease of customization, enabled by the integration of recognition elements and transducers, are key for on-site biosensing for both healthcare and industry and for speeding up translation to real-life applications. Here, we provide an overview of recent progress in printed microfluidics, from the 2D to the 4D level, accompanied by novel sensing element integration. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01677799193005
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http://dx.doi.org/10.1016/j.tibtech.2019.03.009DOI Listing
April 2019
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Real-Time Measurement of Melanoma Cell-Mediated Human Brain Endothelial Barrier Disruption Using Electric Cell-Substrate Impedance Sensing Technology.

Biosensors (Basel) 2019 Apr 15;9(2). Epub 2019 Apr 15.

Department of Molecular Medicine and Pathology, School of Medical Sciences, Faculty of Medical and Health Sciences, University of Auckland, Auckland 1023, New Zealand.

Electric cell-substrate impedance sensing (ECIS) is an impedance-based method for monitoring changes in cell behaviour in real-time. In this paper, we highlight the importance of ECIS in measuring the kinetics of human melanoma cell invasion across human brain endothelium. ECIS data can be mathematically modelled to assess which component of the endothelial paracellular and basolateral barriers is being affected and when. Read More

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http://dx.doi.org/10.3390/bios9020056DOI Listing

Recent Advances on Electrochemical Biosensing Strategies toward Universal Point of Care Systems.

Angew Chem Int Ed Engl 2019 Apr 16. Epub 2019 Apr 16.

Case Western Reserve University, Chemical and Biomolecular Engineering, 10900 Euclid Ave, 44106, Cleveland, UNITED STATES.

A number of very recently developed electrochemical biosensing strategies are promoting electrochemical biosensing systems into practical point-of-care applications. Focus of research endeavors in biosensing community has transferred from detection of a specific analyte to the development of general biosensing strategies that can be applied for a single category of analytes, such as nucleic acid, protein and cell. In this review, we describe most recent cutting-edge researches on electrochemical biosensing strategies. Read More

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http://doi.wiley.com/10.1002/anie.201901879
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http://dx.doi.org/10.1002/anie.201901879DOI Listing
April 2019
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A new device concept for bacterial sensing by Raman spectroscopy and voltage-gated monolayer graphene.

Nanoscale 2019 Apr 16. Epub 2019 Apr 16.

Department of Chemistry, Myongji University, Yongin, South Korea.

Electron-phonon coupling in monolayer graphene results in a modification of its Raman spectra upon charge transfer processes induced by interaction with its chemical environment or the presence of strain or defects in its structure. Modification of Raman spectra is examined in order to develop ultra-sensitive biosensing techniques for the detection, identification, differentiation and classification of bacteria associated with infectious diseases. Specifically, the electrochemical properties of top gated monolayer graphene on SiO2/Si substrates, in the absence and presence of interaction with Gram-positive bacteria (Enterococcus faecalis, Bacillus subtilis) and Gram-negative bacteria (Escherichia coli and Salmonella typhimurium), are probed by Raman spectroscopy in an applied voltage range from 0 V to 3 V. Read More

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http://dx.doi.org/10.1039/c8nr10010aDOI Listing
April 2019
2 Reads

POMOFs/SWNTs nanocomposite with prominent peroxidase-mimicking activity for L-cysteine 'on-off switch' colorimetric biosensing.

ACS Appl Mater Interfaces 2019 Apr 16. Epub 2019 Apr 16.

In order to explore novel colorimetric biosensors with high sensibility and selectivity, two new Keggin-type POMs based Cu-trz (1,2,4-triazole) MOFs with appropriate surface areas and rich active sites were successfully fabricated, then single-walled carbon nanotubes (SWNTs) were introduced into new POMOFs to fabricate POMOFs/SWNTs nanocomposites. Herein, POMOFs/SWNTs nanocomposites as peroxidase mimics were explored for the first time, and the peroxidase-mimicking catalytic activity of the prepared POMOFs/SWNTs nanocomposites is heavily dependent on the mass ratio of POMOFs and SWNTs, in which the maximum activity is achieved at the mass ratio of 2.5 : 1 (named as PMNT-2). Read More

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http://dx.doi.org/10.1021/acsami.9b00872DOI Listing

Probing Local Folding Allows for Robust Metal Sensing based on a Na+-specific DNAzyme.

Chembiochem 2019 Apr 15. Epub 2019 Apr 15.

University of Waterloo, Department of Chemistry, 200 University Avenue West, N2L 3G1, Waterloo, CANADA.

Fluorescent metal sensors based on DNA often rely on changes in end-to-end distance or local environmental near fluorophore labels. Since metal ions can also non-specifically interact with DNA via various mechanisms such as charge screening, base binding, and increase or decrease duplex stability, robust and specific sensing of metal ions has been quite challenging. In this work, we performed a side-by-side comparison of two signaling strategies on a Na+-specific DNAzyme that contained a Na+ binding aptamer. Read More

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https://onlinelibrary.wiley.com/doi/abs/10.1002/cbic.2019001
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http://dx.doi.org/10.1002/cbic.201900143DOI Listing
April 2019
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Specific and Generic Immunorecognition of Glycopeptide Antibiotics Promoted by Unique and Multiple Orientations of Hapten.

Biosensors (Basel) 2019 Apr 4;9(2). Epub 2019 Apr 4.

Faculty of Chemistry, M. V. Lomonosov MSU, Leninsky Gory, 1, 119991 Moscow, Russia.

Conjugation chemistry does not always provide adequate spatial orientation of hapten in immunogens for the best presentation of generic or individual epitopes. In the present study, the influence of unique and multiple orientations of immunizing hapten on the immune response repertoire was compared to select generic recognition system. The glycopeptides, teicoplanin (TPL) and ristomycin (RSM), were conjugated to BSA to produce immunogens with unique and multiple orientations of haptens. Read More

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http://dx.doi.org/10.3390/bios9020052DOI Listing

Functionalized Carbon Materials for Electronic Devices: A Review.

Micromachines (Basel) 2019 Apr 3;10(4). Epub 2019 Apr 3.

Department of Chemistry, Inha University, 100 Inharo, Incheon 22212, Korea.

Carbon-based materials, including graphene, single walled carbon nanotubes (SWCNTs), and multi walled carbon nanotubes (MWCNTs), are very promising materials for developing future-generation electronic devices. Their efficient physical, chemical, and electrical properties, such as high conductivity, efficient thermal and electrochemical stability, and high specific surface area, enable them to fulfill the requirements of modern electronic industries. In this review article, we discuss the synthetic methods of different functionalized carbon materials based on graphene oxide (GO), SWCNTs, MWCNTs, carbon fibers (CFs), and activated carbon (AC). Read More

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http://dx.doi.org/10.3390/mi10040234DOI Listing

Evaluation of a robust engineered enzyme towards organophosphorus insecticide bioremediation using planarians as biosensors.

Chem Biol Interact 2019 Apr 12;306:96-103. Epub 2019 Apr 12.

Aix Marseille Université, IRD, APHM, MEPHI, IHU-Méditerranée Infection, Marseille, France. Electronic address:

Organophosphorus compounds (OPs) are neurotoxic molecules developed as insecticides and chemical warfare nerve agents (CWNAs). They are covalent inhibitors of acetylcholinesterase (AChE), a key enzyme in central and peripheral nervous systems and are responsible for numerous poisonings worldwide. Many animal models have been studied over the years but finding a suitable in vivo model to account for both acute toxicity and long-term exposure remains a topical issue. Read More

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http://dx.doi.org/10.1016/j.cbi.2019.04.013DOI Listing

Click-conjugated photon-upconversion nanoparticles in an immunoassay for honeybee pathogen Melissococcus plutonius.

Nanoscale 2019 Apr 15. Epub 2019 Apr 15.

CEITEC MU, Masaryk University, Kamenice 5, 625 00 Brno, Czech Republic.

European foulbrood (EFB) is an infectious disease affecting honeybee larvae caused by the bacterium Melissococcus plutonius. The enzyme-linked immunosorbent assay (ELISA) is the gold standard for antibody-based bacteria detection, however, its sensitivity is not high enough to reveal early-stage EFB infection. Photon-upconversion nanoparticles (UCNPs) are lanthanide-doped nanomaterials that emit light of shorter wavelength under near-infrared (NIR) excitation and thus avoid optical background interference. Read More

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http://dx.doi.org/10.1039/c9nr01246jDOI Listing

Nanoplasmonics in Paper-Based Analytical Devices.

Front Bioeng Biotechnol 2019 29;7:69. Epub 2019 Mar 29.

Biophotonic Nanosensors Laboratory, Centro de Investigaciones en Óptica A. C., León, Mexico.

Chemical and biological sensing are crucial tools in science and technology. Plasmonic nanoparticles offer a virtually limitless number of photons for sensing applications, which can be available for visual detection over long periods. Moreover, cellulosic materials, such as paper, represent a versatile building block for implementation of simple, yet valuable, microfluidic analytical devices. Read More

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http://dx.doi.org/10.3389/fbioe.2019.00069DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6449474PMC

AKAP95 Organizes a Nuclear Microdomain to Control Local cAMP for Regulating Nuclear PKA.

Cell Chem Biol 2019 Mar 21. Epub 2019 Mar 21.

Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA; Department of Pharmacology, University of California, 9500 Gilman Drive, BRF-II 1120, La Jolla, CA 92093-0702, USA. Electronic address:

Contrary to the classic model of protein kinase A (PKA) residing outside of the nucleus, we identify a nuclear signaling complex that consists of AKAP95, PKA, and PDE4D5 and show that it forms a functional cyclic AMP (cAMP) signaling microdomain. Locally generated cAMP can accumulate within the vicinity of this complex; however, when cAMP is generated at the plasma membrane, PDE4 serves as a local sink and PDE3 as a barrier to prevent accumulation of cAMP within the microdomain as a means of controlling activation of tethered nuclear PKA. Read More

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http://dx.doi.org/10.1016/j.chembiol.2019.03.003DOI Listing

Sensitive electrochemical biosensor for MicroRNAs based on duplex-specific nuclease-assisted target recycling followed with gold nanoparticles and enzymatic signal amplification.

Anal Chim Acta 2019 Aug 15;1064:33-39. Epub 2019 Mar 15.

Jiangsu Key Laboratory of New Power Batteries, Jiangsu Collaborative Innovation Center of Biomedical Functional Materials, Jiangsu Key Laboratory of Biomedical Materials, National and Local Joint Engineering Research Center of Biomedical Functional Materials, College of Chemistry and Materials Science, Nanjing Normal University, Nanjing, 210023, PR China. Electronic address:

The detection of sequence-specific microRNAs (miRNAs) is an important factor to the diseases diagnosis. Herein, a triple signal amplification electrochemical biosensor for highly sensitive detection of miRNA-21 was developed based on a duplex-specific nuclease (DSN)-assisted target recycling combined with gold nanoparticles (NPs), horseradish peroxidase (HRP) enzymatic signal amplification. The electrochemical biosensor generated significantly amplified amperometric current changes (Δi) for the detection of miRNA-21 down to 43. Read More

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http://dx.doi.org/10.1016/j.aca.2019.02.060DOI Listing
August 2019
2 Reads

Theoretical investigation of size, shape, and aspect ratio effect on the LSPR sensitivity of hollow-gold nanoshells.

J Chem Phys 2019 Apr;150(14):144116

Department of Chemistry, Western Michigan University, Kalamazoo, Michigan 49008, USA.

The change in refractive index around plasmonic nanoparticles upon binding to biomolecules is routinely used in localized surface plasmon resonance (LSPR)-based biosensors and in biosensing platforms. In this study, the plasmon sensitivity of hollow gold (Au) nanoshells is studied using theoretical modeling where the influence of shape, size, shell thickness, and aspect ratio is addressed. Different shapes of hollow Au nanoshells are studied that include sphere, disk, triangular prism, rod, ellipsoid, and rectangular block. Read More

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http://dx.doi.org/10.1063/1.5090885DOI Listing

Plug-and-play metabolic transducers expand the chemical detection space of cell-free biosensors.

Nat Commun 2019 Apr 12;10(1):1697. Epub 2019 Apr 12.

Centre de Biochimie Structurale, INSERM U1054, CNRS UMR5048, University of Montpellier, Montpellier, 34090, France.

Cell-free transcription-translation systems have great potential for biosensing, yet the range of detectable chemicals is limited. Here we provide a workflow to expand the range of molecules detectable by cell-free biosensors through combining synthetic metabolic cascades with transcription factor-based networks. These hybrid cell-free biosensors have a fast response time, strong signal response, and a high dynamic range. Read More

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http://www.nature.com/articles/s41467-019-09722-9
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http://dx.doi.org/10.1038/s41467-019-09722-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6461607PMC
April 2019
2 Reads

Transmit-Array, Metasurface-Based Tunable Polarizer and High-Performance Biosensor in the Visible Regime.

Nanomaterials (Basel) 2019 Apr 11;9(4). Epub 2019 Apr 11.

School of Physics, University of Electronic Science and Technology of China, Chengdu 610054, China.

There are two types of metasurfaces, reflect-array and transmit-array,-which are classified on the basis of structural features. In this paper, we design a transmit-array metasurface for -polarized incidence which is characterized by having a transmission spectrum with a narrow dip (i.e. Read More

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https://www.mdpi.com/2079-4991/9/4/603
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http://dx.doi.org/10.3390/nano9040603DOI Listing
April 2019
4 Reads

Effect of control mode on the sensitivity of a microbial fuel cell biosensor with Shewanella loihica PV-4 and the underlying bioelectrochemical mechanism.

Bioelectrochemistry 2019 Apr 2;128:109-117. Epub 2019 Apr 2.

Beijing Advanced Innovation Center for Biomedical Engineering, Beihang University, Beijing 100191, China; Institute of Environmental Biology and Life Support Technology, School of Biological Science and Medical Engineering, Beihang University, Beijing 100191, China; International Joint Research Center of Aerospace Biotechnology & Medical Engineering, Beihang University, Beijing 100191, China. Electronic address:

A relatively poor sensitivity is a critical challenge for the application of microbial fuel cell biosensors (MFC-biosensors). This study investigated the effects of two control modes on sensor sensitivity and revealed the underlying bioelectrochemical mechanism. The results demonstrated that the sensitivity of an S. Read More

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http://dx.doi.org/10.1016/j.bioelechem.2019.04.001DOI Listing
April 2019
2 Reads

Advanced multi-modal diagnostic approaches for detection of lung cancer.

Expert Rev Mol Diagn 2019 Apr 12. Epub 2019 Apr 12.

a Shobhaben Pratapbhai Patel School of Pharmacy and Technology Management , Shri Vile Parle Kelavani Mandal'S Narsee Monjee Institute of Management Studies University , V.L. Mehta Road, Vile Parle (W), Mumbai , Maharashtra 400056 , India .

Introduction: Lung cancer (LC) emerges as a principle cause of death among smokers and is also one of the most lethal forms of cancer in non-smokers. LC is mainly classified as non-small cell lung cancer (NSCLC), small cell lung cancer and lung carcinoid tumor. NSCLC is the most prevalent form of LC and its early stage diagnosis is essential to reduce mortality rate of patients and provide specific therapy. Read More

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https://www.tandfonline.com/doi/full/10.1080/14737159.2019.1
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http://dx.doi.org/10.1080/14737159.2019.1607299DOI Listing
April 2019
2 Reads

Electrodes for membrane surface science. Bilayer lipid membranes tethered by commercial surfactants on electrochemical sensors.

ACS Sens 2019 Apr 12. Epub 2019 Apr 12.

Commercial surfactants, inexpensive and abundant, were covalently grafted to flat and transparent electrodes and it appears to be a simple functionalization route to design bio-membrane sensors at large scale production. Sparsely tethered bilayer lipid membranes (stBLM) were stabilized from such molecular coatings composed of diluted anchor-harpoon surfactants that grab the membrane with an alkyl chain out of a PEGylated-hydrogel layer which acts as a soft hydration cushion. Avoiding the synthesis of complex organic molecules to scale up sensors realization was achieved here by grafting non-ionic diblock oligo-mers (Brij58: CxH2x+1(OCH2CH2)nOH with x=16 and n=23), as well as PEO short chains ((OCH2CH2)nOH with n=9 and n=23) from their hydroxyl (-OH) end-moiety to a monolayer of -Ar-SO2Cl groups, easy to form on electrodes (metals, semi-conducting materials, …) from aryl-diazonium salts reduction. Read More

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http://dx.doi.org/10.1021/acssensors.9b00267DOI Listing
April 2019
2 Reads

Aptamer based nanobiosensors: Promising healthcare devices.

Saudi Pharm J 2019 Mar 24;27(3):312-319. Epub 2018 Nov 24.

Department of Pharmacology, College of Pharmacy, Prince Sattam Bin Abdulaziz University, Al-Kharj 11942, Saudi Arabia.

Nanobiosensors based on aptamer are extensively being studied as potent analytical tools in clinical analysis. These biosensors provide high sensitivity, fast response, specificity and desired portability in addition to simplicity and decreased cost compared to conventional methods. The purpose of this manuscript is to provide readers with an overview of current advances about electrochemical, electrochemiluminescent and photoelectrochemical aptasensors from the sea of available literature. Read More

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http://dx.doi.org/10.1016/j.jsps.2018.11.013DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6438676PMC
March 2019
1 Read
1.000 Impact Factor

Measuring the Permeability of Endothelial Cell Monolayers: Teaching New Tricks to an Old Dog.

Authors:
Joachim Wegener

Biophys J 2019 Mar 28. Epub 2019 Mar 28.

Institute of Analytical Chemistry, Chemo- and Biosensors, University of Regensburg, Regensburg, Germany; Fraunhofer Research Institution for Microsystems and Solid State Technologies EMFT, Munich, Germany. Electronic address:

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http://dx.doi.org/10.1016/j.bpj.2019.03.018DOI Listing

Robust SERS Platforms Based on Annealed Gold Nanostructures Formed on Ultrafine Glass Substrates for Various (Bio)Applications.

Biosensors (Basel) 2019 Apr 10;9(2). Epub 2019 Apr 10.

Light, Nanomaterials and Nanotechnology (L2N), FRE-CNRS 2019, Institute Charles Delaunay (ICD), University of Technology of Troyes, 12 Rue Marie Curie CS 42060, 10004 Troyes CEDEX, France.

In this study, stable gold nanoparticles (AuNPs) are fabricated for the first time on commercial ultrafine glass coverslips coated with gold thin layers (2 nm, 4 nm, 6 nm, and 8 nm) at 25 °C and annealed at high temperatures (350 °C, 450 °C, and 550 °C) on a hot plate for different periods of time. Such gold nanostructured coverslips were systematically tested via surface enhanced Raman spectroscopy (SERS) to identify their spectral performances in the presence of different concentrations of a model molecule, namely 1,2-bis-(4-pyridyl)-ethene (BPE). By using these SERS platforms, it is possible to detect BPE traces (10 M) in aqueous solutions in 120 s. Read More

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http://dx.doi.org/10.3390/bios9020053DOI Listing

Towards Portable Nanophotonic Sensors.

Sensors (Basel) 2019 Apr 10;19(7). Epub 2019 Apr 10.

School of Engineering, University of Glasgow, Glasgow G12 8LT, UK.

A range of nanophotonic sensors composed of different materials and device configurations have been developed over the past two decades. These sensors have achieved high performance in terms of sensitivity and detection limit. The size of onchip nanophotonic sensors is also small and they are regarded as a strong candidate to provide the next generation sensors for a range of applications including chemical and biosensing for point-of-care diagnostics. Read More

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http://dx.doi.org/10.3390/s19071715DOI Listing

Prêt-à-porter nanoYESα and nanoYESβ bioluminescent cell biosensors for ultrarapid and sensitive screening of endocrine-disrupting chemicals.

Anal Bioanal Chem 2019 Apr 10. Epub 2019 Apr 10.

Department of Chemistry "G. Ciamician", University of Bologna, Via Selmi 2, 40126, Bologna, Italy.

Cell-based assays utilizing reporter gene technology have been widely exploited for biosensing, as they provide useful information about the bioavailability and cell toxicity of target analytes. The long assay time due to gene transcription and translation is one of the main drawbacks of cell biosensors. We report the development of two yeast biosensors stably expressing human estrogen receptors α and β and employing NanoLuc as the reporter protein to upgrade the widely used yeast estrogen screening (YES) assays. Read More

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http://dx.doi.org/10.1007/s00216-019-01805-2DOI Listing

Biosensors Using an Antibody as a Recognition Element.

Anal Sci 2019 ;35(4):359-360

Graduate School of Material Science, University of Hyogo.

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http://dx.doi.org/10.2116/analsci.highlights1904DOI Listing
January 2019

Quantification and Comparison of Signals Generated by Different FRET-Based cAMP Reporters.

Methods Mol Biol 2019 ;1947:217-237

Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford, UK.

A variety of FRET-based biosensors are currently in use for real-time monitoring of dynamic changes of intracellular cAMP. Due to differences in sensor properties, unique features of the cell type under examination and diverse specifications of the imaging setups in different laboratories, data generated using these sensors may not be immediately comparable within the same study or across studies. To facilitate comparison, often FRET data are normalized and expressed as fractional change of the maximal FRET response at sensor saturation. Read More

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http://link.springer.com/10.1007/978-1-4939-9121-1_12
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http://dx.doi.org/10.1007/978-1-4939-9121-1_12DOI Listing
January 2019
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Combining Conformational Profiling of GPCRs with CRISPR/Cas9 Gene Editing Approaches.

Methods Mol Biol 2019 ;1947:169-182

Department of Pharmacology and Therapeutics, McGill University, Montreal, QC, Canada.

Ligand-biased signaling could have a significant impact on drug discovery programs. As such, many approaches to screening now target a larger section of the signaling responses downstream of an individual G protein-coupled receptor (GPCR). Biosensor-based platforms have been developed to capture signaling signatures. Read More

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http://link.springer.com/10.1007/978-1-4939-9121-1_9
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http://dx.doi.org/10.1007/978-1-4939-9121-1_9DOI Listing
January 2019
1 Read

Recent Progress of Imprinted Polymer Photonic Waveguide Devices and Applications.

Polymers (Basel) 2018 May 31;10(6). Epub 2018 May 31.

School of Optoelectronic Engineering and Instrumentation Science, Dalian University of Technology, Dalian 116024, China.

Polymers are promising materials for fabricating photonic integrated waveguide devices. Versatile functional devices can be manufactured using a simple process, with low cost and potential mass-manufacturing. This paper reviews the recent progress of polymer photonic integrated devices fabricated using the UV imprinting technique. Read More

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http://www.mdpi.com/2073-4360/10/6/603
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http://dx.doi.org/10.3390/polym10060603DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6404155PMC
May 2018
2 Reads

Chemical Design of Functional Polymer Structures for Biosensors: From Nanoscale to Macroscale.

Polymers (Basel) 2018 May 21;10(5). Epub 2018 May 21.

Department of Polymer Engineering, Graduate School, Chonnam National University, 77 Yongbong-ro, Buk-gu, Gwangju 61186, Korea.

Over the past decades, biosensors, a class of physicochemical detectors sensitive to biological analytes, have drawn increasing interest, particularly in light of growing concerns about human health. Functional polymeric materials have been widely researched for sensing applications because of their structural versatility and significant progress that has been made concerning their chemistry, as well as in the field of nanotechnology. Polymeric nanoparticles are conventionally used in sensing applications due to large surface area, which allows rapid and sensitive detection. Read More

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http://dx.doi.org/10.3390/polym10050551DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6415446PMC

DNA aptamers for the recognition of HMGB1 from Plasmodium falciparum.

PLoS One 2019 9;14(4):e0211756. Epub 2019 Apr 9.

Laboratory of Applied Biophysics and Biochemistry, Centre for Research and Innovation, Health Sciences Faculty, Universidad Peruana de Ciencias Aplicadas (UPC), Lima, Perú.

Rapid Diagnostic Tests (RDTs) for malaria are restricted to a few biomarkers and antibody-mediated detection. However, the expression of commonly used biomarkers varies geographically and the sensibility of immunodetection can be affected by batch-to-batch differences or limited thermal stability. In this study we aimed to overcome these limitations by identifying a potential biomarker and by developing molecular sensors based on aptamer technology. Read More

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0211756PLOS

Manganese as a Catalytic Mediator for Photo-oxidation and Breaking the pH Limitation of Nanozymes.

Nano Lett 2019 Apr 12. Epub 2019 Apr 12.

Department of Chemistry , Waterloo Institute for Nanotechnology , Waterloo , Ontario , Canada N2L 3G1.

A long-standing challenge in nanozyme catalysis is low activity at physiological pH, especially for oxidase- and peroxidase-mimicking nanozymes. We herein communicate that Mn(II) can promote catalysis at neutral pH for carbon dots (C-dots) as a photo-oxidase nanozyme. The C-dots produce singlet oxygen upon light irradiation to oxidize Mn(II) to Mn(III), which is confirmed by a suite of spectroscopic evidence. Read More

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http://dx.doi.org/10.1021/acs.nanolett.9b00725DOI Listing

Monitoring of Microphysiological Systems: Integrating Sensors and Real-Time Data Analysis toward Autonomous Decision-Making.

ACS Sens 2019 Apr 19. Epub 2019 Apr 19.

Joint Department of Biomedical Engineering , North Carolina State University and University of North Carolina, Chapel Hill , 911 Oval Drive , Raleigh , North Carolina 27695 , United States.

Microphysiological systems replicate human organ function and are promising technologies for discovery of translatable biomarkers, pharmaceuticals, and regenerative therapies. Because microphysiological systems require complex microscale anatomical structures and heterogeneous cell populations, a major challenge remains to manufacture and operate these products with reproducible and standardized function. In this Perspective, three stages of microphysiological system monitoring, including process, development, and function, are assessed. Read More

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http://dx.doi.org/10.1021/acssensors.8b01549DOI Listing

Decoding the Time-Dependent Response of Bioluminescent Metal-Detecting Whole-Cell Bacterial Sensors.

ACS Sens 2019 Apr 19. Epub 2019 Apr 19.

Université de Lorraine , CNRS, LIEC, UMR 7360, Campus Bridoux, Metz F-57070 , France.

The signal produced by aqueous dispersions of bioluminescent, metal-responsive whole-cell bacterial sensors is indicative of the concentration of bioavailable metal ions in solution. The conventional calibration-based strategy followed for measuring this concentration is however inadequate to provide any quantitative prediction of the cell response over time as a function of, e.g. Read More

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http://pubs.acs.org/doi/10.1021/acssensors.9b00349
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http://dx.doi.org/10.1021/acssensors.9b00349DOI Listing
April 2019
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Experimental and theoretical investigation of sensing parameters in carbon nanotube-based DNA sensor.

IET Nanobiotechnol 2018 Dec;12(8):1125-1129

Electronics and Computer Engineering Department, Faculty of Electrical Engineering, Universiti Teknologi Malaysia, Johor Bahru, Malaysia.

Nowadays, sensitive biosensors with high selectivity, lower costs and short response time are required for detection of DNA. The most preferred materials in DNA sensor designing are nanomaterials such as carbon and Au nanoparticles, because of their very high surface area and biocompatibility which lead to performance and sensitivity improvements in DNA sensors. Carbon nanomaterials such as carbon nanotubes (CNTs) can be considered as a suitable DNA sensor platform due to their high surface-to-volume ratio, favourable electronic properties and fast electron transfer rate. Read More

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http://dx.doi.org/10.1049/iet-nbt.2018.5068DOI Listing
December 2018

Distinct cortical-amygdala projections drive reward value encoding and retrieval.

Nat Neurosci 2019 Apr 8. Epub 2019 Apr 8.

Department of Psychology, University of California, Los Angeles, Los Angeles, CA, USA.

The value of an anticipated rewarding event is a crucial component of the decision to engage in its pursuit. But little is known of the networks responsible for encoding and retrieving this value. By using biosensors and pharmacological manipulations, we found that basolateral amygdala (BLA) glutamatergic activity tracks and mediates encoding and retrieval of the state-dependent incentive value of a palatable food reward. Read More

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http://dx.doi.org/10.1038/s41593-019-0374-7DOI Listing

Specific inhibition of splicing factor activity by decoy RNA oligonucleotides.

Nat Commun 2019 04 8;10(1):1590. Epub 2019 Apr 8.

Department of Biochemistry and Molecular Biology, Institute for Medical Research Israel-Canada, Hebrew University-Hadassah Medical School, Jerusalem, 9112001, Israel.

Alternative splicing, a fundamental step in gene expression, is deregulated in many diseases. Splicing factors (SFs), which regulate this process, are up- or down regulated or mutated in several diseases including cancer. To date, there are no inhibitors that directly inhibit the activity of SFs. Read More

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http://dx.doi.org/10.1038/s41467-019-09523-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6453957PMC

Quantifying Mg Binding to ssDNA Oligomers: A Self-Consistent Field Theory Study at Varying Ionic Strengths and Grafting Densities.

Polymers (Basel) 2018 Dec 18;10(12). Epub 2018 Dec 18.

Department of Chemical Engineering, University of South Carolina, Columbia, SC 29208, USA.

The performance of aptamer-based biosensors is crucially impacted by their interactions with physiological metal ions, which can alter their structures and chemical properties. Therefore, elucidating the nature of these interactions carries the utmost importance in the robust design of highly efficient biosensors. We investigated Mg 2 + binding to varying sequences of polymers to capture the effects of ionic strength and grafting density on ion binding and molecular reorganization of the polymer layer. Read More

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http://dx.doi.org/10.3390/polym10121403DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6401855PMC
December 2018