505 results match your criteria Biosensors[Journal]


Real-Time Measurement of Melanoma Cell-Mediated Human Brain Endothelial Barrier Disruption Using Electric Cell-Substrate Impedance Sensing Technology.

Biosensors (Basel) 2019 Apr 15;9(2). Epub 2019 Apr 15.

Department of Molecular Medicine and Pathology, School of Medical Sciences, Faculty of Medical and Health Sciences, University of Auckland, Auckland 1023, New Zealand.

Electric cell-substrate impedance sensing (ECIS) is an impedance-based method for monitoring changes in cell behaviour in real-time. In this paper, we highlight the importance of ECIS in measuring the kinetics of human melanoma cell invasion across human brain endothelium. ECIS data can be mathematically modelled to assess which component of the endothelial paracellular and basolateral barriers is being affected and when. Read More

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http://dx.doi.org/10.3390/bios9020056DOI Listing

Specific and Generic Immunorecognition of Glycopeptide Antibiotics Promoted by Unique and Multiple Orientations of Hapten.

Biosensors (Basel) 2019 Apr 4;9(2). Epub 2019 Apr 4.

Faculty of Chemistry, M. V. Lomonosov MSU, Leninsky Gory, 1, 119991 Moscow, Russia.

Conjugation chemistry does not always provide adequate spatial orientation of hapten in immunogens for the best presentation of generic or individual epitopes. In the present study, the influence of unique and multiple orientations of immunizing hapten on the immune response repertoire was compared to select generic recognition system. The glycopeptides, teicoplanin (TPL) and ristomycin (RSM), were conjugated to BSA to produce immunogens with unique and multiple orientations of haptens. Read More

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http://dx.doi.org/10.3390/bios9020052DOI Listing

Robust SERS Platforms Based on Annealed Gold Nanostructures Formed on Ultrafine Glass Substrates for Various (Bio)Applications.

Biosensors (Basel) 2019 Apr 10;9(2). Epub 2019 Apr 10.

Light, Nanomaterials and Nanotechnology (L2N), FRE-CNRS 2019, Institute Charles Delaunay (ICD), University of Technology of Troyes, 12 Rue Marie Curie CS 42060, 10004 Troyes CEDEX, France.

In this study, stable gold nanoparticles (AuNPs) are fabricated for the first time on commercial ultrafine glass coverslips coated with gold thin layers (2 nm, 4 nm, 6 nm, and 8 nm) at 25 °C and annealed at high temperatures (350 °C, 450 °C, and 550 °C) on a hot plate for different periods of time. Such gold nanostructured coverslips were systematically tested via surface enhanced Raman spectroscopy (SERS) to identify their spectral performances in the presence of different concentrations of a model molecule, namely 1,2-bis-(4-pyridyl)-ethene (BPE). By using these SERS platforms, it is possible to detect BPE traces (10 M) in aqueous solutions in 120 s. Read More

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http://dx.doi.org/10.3390/bios9020053DOI Listing

A Novel Toolkit for Characterizing the Mechanical and Electrical Properties of Engineered Neural Tissues.

Biosensors (Basel) 2019 Apr 1;9(2). Epub 2019 Apr 1.

Department of Mechanical Engineering, University of Victoria, Victoria, B.C. V8W 2Y2, Canada.

We have designed and validated a set of robust and non-toxic protocols for directly evaluating the properties of engineered neural tissue. These protocols characterize the mechanical properties of engineered neural tissues and measure their electrophysical activity. The protocols obtain elastic moduli of very soft fibrin hydrogel scaffolds and voltage readings from motor neuron cultures. Read More

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http://dx.doi.org/10.3390/bios9020051DOI Listing
April 2019
1 Read

ation of the ighensitiv Label-Free .

Biosensors (Basel) 2019 Mar 31;9(2). Epub 2019 Mar 31.

Department of Chemical and Biomolecular Engineering, NYU Tandon School of Engineering, Six Metrotech Center, Brooklyn, NY 11201, USA.

A highly sensitive electrochemical biosensor with a signal amplification platform of electrodeposited gold nanoparticle (AuNP) has been developed and characterized. The sizes of the synthesized AuNP were found to be critical for the performance of biosensor in which the sizes were dependent on HAuCl₄ and acid concentrations; as well as on scan cycles and scan rates in the gold electro-reduction step. Systematic investigations of the adsorption of proteins with different sizes from aqueous electrolyte solution onto the electrodeposited AuNP surface were performed with a potentiometric method and calibrated by design of experiment (DOE). Read More

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http://dx.doi.org/10.3390/bios9020050DOI Listing
March 2019
2 Reads

Raman Spectrochemical Analysis Using a Handheld Probe Demonstrates High Predictive Capability of Brain Tumour Status.

Biosensors (Basel) 2019 Mar 30;9(2). Epub 2019 Mar 30.

School of Pharmacy and Biomedical Sciences, University of Central Lancashire, Preston PR1 2HE, UK.

With brain tumour incidence increasing, there is an urgent need for better diagnostic tools. Intraoperatively, brain tumours are diagnosed using a smear preparation reported by a neuropathologist. These have many limitations, including the time taken for the specimen to reach the pathology department and for results to be communicated to the surgeon. Read More

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http://dx.doi.org/10.3390/bios9020049DOI Listing

Comparative Design Study for Power Reduction in Organic Optoelectronic Pulse Meter Sensor.

Biosensors (Basel) 2019 Mar 29;9(2). Epub 2019 Mar 29.

Department of Applied Science for Electronics and Materials, Kyushu University, Fukuoka 816-8580, Japan.

This paper demonstrated a new design structure for minimizing the power consumption of a pulse meter. Monolithic devices composed of a red (625 nm) organic light-emitting diode (OLED) and an organic photodiode (OPD) were fabricated on the same substrate. Two organic devices were designed differently. Read More

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https://www.mdpi.com/2079-6374/9/2/48
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http://dx.doi.org/10.3390/bios9020048DOI Listing
March 2019
4 Reads

A Review of the Construction of Nano-Hybrids for Electrochemical Biosensing of Glucose.

Biosensors (Basel) 2019 Mar 25;9(1). Epub 2019 Mar 25.

BAE: Biocapteurs-Analyses-Environnement, Universite de Perpignan ViaDomitia, 52 Avenue Paul Alduy, 66860 Perpignan CEDEX, France.

Continuous progress in the domain of nano and material science has led to modulation of the properties of nanomaterials in a controlled and desired fashion. In this sense, nanomaterials, including carbon-based materials, metals and metal oxides, and composite/hybrid materials have attracted extensive interest with regard to the construction of electrochemical biosensors. The modification of a working electrode with a combination of two or three nanomaterials in the form of nano-composite/nano-hybrids has revealed good results with very good reproducibility, stability, and improved sensitivity. Read More

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http://dx.doi.org/10.3390/bios9010046DOI Listing

Electrochemical Detection and Characterization of Nanoparticles with Printed Devices.

Biosensors (Basel) 2019 Mar 28;9(2). Epub 2019 Mar 28.

Department of Chemical Engineering, KTH Royal Institute of Technology, 100-44 Stockholm, Sweden.

Innovative methods to achieve the user-friendly, quick, and highly sensitive detection of nanomaterials are urgently needed. Nanomaterials have increased importance in commercial products, and there are concerns about the potential risk that they entail for the environment. In addition, detection of nanomaterials can be a highly valuable tool in many applications, such as biosensing. Read More

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https://www.mdpi.com/2079-6374/9/2/47
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http://dx.doi.org/10.3390/bios9020047DOI Listing
March 2019
1 Read

Electrochemical Response of Corresponds to Cell Viability upon Exposure to Seed Extracts and Antifungal Drugs.

Biosensors (Basel) 2019 Mar 20;9(1). Epub 2019 Mar 20.

West African Centre for Cell Biology of Infectious Pathogens, University of Ghana, Legon P.O. Box LG 54, Ghana.

bioactive compounds have been shown to contain antioxidant properties. The extracts from the same plant are used in traditional medical practices to treat various diseases with impressive outcomes. In this study, ionic mobility in cells in the presence of seed extracts was monitored using electrochemical detection methods to link cell death to ionic imbalance. Read More

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http://dx.doi.org/10.3390/bios9010045DOI Listing

Continuous Monitoring of pH and Blood Gases Using Ion-Sensitive and Gas-Sensitive Field Effect Transistors Operating in the Amperometric Mode in Presence of Drift.

Authors:
Shahriar Jamasb

Biosensors (Basel) 2019 Mar 18;9(1). Epub 2019 Mar 18.

Department of Biomedical Engineering, Hamedan University of Technology, Hamedan 65169-13733, Iran.

Accurate and cost-effective integrated sensor systems for continuous monitoring of pH and blood gases continue to be in high demand. The capacity of ion-selective and Gas-sensitive field effect transistors (FETs) to serve as low-power sensors for accurate continuous monitoring of pH and blood gases is evaluated in the amperometric or current mode of operation. A stand-alone current-mode topology is employed in which a constant bias is applied to the gate with the drain current serving as the measuring signal. Read More

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http://dx.doi.org/10.3390/bios9010044DOI Listing

Nanostructured Gas Sensors for Medical and Health Applications: Low to High Dimensional Materials.

Biosensors (Basel) 2019 Mar 17;9(1). Epub 2019 Mar 17.

Institute for Biomedical Materials and Devices, Faculty of Science, University of Technology Sydney, Sydney NSW 2007, Australia.

Human breath has long been known as a system that can be used to diagnose diseases. With advancements in modern nanotechnology, gas sensors can now diagnose, predict, and monitor a wide range of diseases from human breath. From cancer to diabetes, the need to treat at the earliest stages of a disease to both increase patient outcomes and decrease treatment costs is vital. Read More

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http://dx.doi.org/10.3390/bios9010043DOI Listing

Integration of Nanomaterials and Bioluminescence Resonance Energy Transfer Techniques for Sensing Biomolecules.

Biosensors (Basel) 2019 Mar 16;9(1). Epub 2019 Mar 16.

School of Biomedical Engineering, University of Western Ontario, 1151 Richmond St., London, ON N6A 5B9, Canada.

Bioluminescence resonance energy transfer (BRET) techniques offer a high degree of sensitivity, reliability and ease of use for their application to sensing biomolecules. BRET is a distance dependent, non-radiative energy transfer, which uses a bioluminescent protein to excite an acceptor through the resonance energy transfer. A BRET sensor can quickly detect the change of a target biomolecule quantitatively without an external electromagnetic field, e. Read More

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http://dx.doi.org/10.3390/bios9010042DOI Listing

Enzyme-Based Electrochemical Biosensors for Microfluidic Platforms to Detect Pharmaceutical Residues in Wastewater.

Biosensors (Basel) 2019 Mar 15;9(1). Epub 2019 Mar 15.

Department of Electrical and Electronics Engineering, Universidad de los Andes, Cra. 1E No. 19a-40, Bogotá, DC 111711, Colombia.

Emerging water pollutants such as pharmaceutical contaminants are suspected to induce adverse effects to human health. These molecules became worrisome due to their increasingly high concentrations in surface waters. Despite this alarming situation, available data about actual concentrations in the environment is rather scarce, as it is not commonly monitored or regulated. Read More

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http://dx.doi.org/10.3390/bios9010041DOI Listing
March 2019
2 Reads

Separation and Detection of and Using a Microfluidic Device Integrated with an Optical Fibre.

Biosensors (Basel) 2019 Mar 14;9(1). Epub 2019 Mar 14.

Department of Chemical and Environmental Engineering, University Putra Malaysia, Selangor 43400, Malaysia.

This paper describes the development of an integrated system using a dry film resistant (DFR) microfluidic channel consisting of pulsed field dielectrophoretic field-flow-fractionation (DEP-FFF) separation and optical detection. The prototype chip employs the pulse DEP-FFF concept to separate the cells ( and ) from a continuous flow, and the rate of release of the cells was measured. The separation experiments were conducted by changing the pulsing time over a pulsing time range of 2⁻24 s and a flow rate range of 1. Read More

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https://www.mdpi.com/2079-6374/9/1/40
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http://dx.doi.org/10.3390/bios9010040DOI Listing
March 2019
2 Reads

An Exfoliated Graphite-Based Electrochemical Immunosensor on a Dendrimer/Carbon Nanodot Platform for the Detection of Carcinoembryonic Antigen Cancer Biomarker.

Biosensors (Basel) 2019 Mar 8;9(1). Epub 2019 Mar 8.

Department of Applied Chemistry, University of Johannesburg, Doornfontein 2028, Johannesburg, South Africa.

An electrochemical immunosensor for the quantification of carcinoembryonic antigen (CEA) using a nanocomposite of polypropylene imine dendrimer (PPI) and carbon nanodots (CNDTs) on an exfoliated graphite electrode (EG) is reported. The carbon nanodots were prepared by pyrolysis of oats. The nanocomposites (PPI and CNDTs) were characterized using X-ray powder diffraction (XRD), Raman spectroscopy, Fourier transform infrared spectroscopy (FTIR), high-resolution transmission electron microscopy (HRTEM) and scanning electron microscopy (SEM). Read More

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http://dx.doi.org/10.3390/bios9010039DOI Listing
March 2019
2 Reads

Electrochemical Biosensors as Potential Diagnostic Devices for Autoimmune Diseases.

Biosensors (Basel) 2019 Mar 4;9(1). Epub 2019 Mar 4.

Analytical Chemistry Department, Iuliu Haţieganu University of Medicine and Pharmacy, 400349 Cluj-Napoca, Romania.

An important class of biosensors is immunosensors, affinity biosensors that are based on the specific interaction between antibodies and antigens. They are classified in four classes based on the type of employed transducer: electrochemical, optical, microgravimetric, and thermometric and depending on the type of recognition elements, antibodies, aptamers, microRNAs and recently peptides are integrating parts. Those analytical devices are able to detect peptides, antibodies and proteins in various sample matrices, without many steps of sample pretreatment. Read More

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http://dx.doi.org/10.3390/bios9010038DOI Listing
March 2019
3 Reads

Waveguiding and SERS Simplified Raman Spectroscopy on Biological Samples.

Biosensors (Basel) 2019 Mar 3;9(1). Epub 2019 Mar 3.

Department of Experimental and Clinical Medicine, University "Magna Graecia" of Catanzaro, 88100 Catanzaro, Italy.

Biomarkers detection at an ultra-low concentration in biofluids (blood, serum, saliva, etc.) is a key point for the early diagnosis success and the development of personalized therapies. However, it remains a challenge due to limiting factors like () the complexity of analyzed media, and () the aspecificity detection and the poor sensitivity of the conventional methods. Read More

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http://dx.doi.org/10.3390/bios9010037DOI Listing
March 2019
12 Reads

Solid-State rGO-PEDOT:PSS Transducing Material for Cost-Effective Enzymatic Sensing.

Biosensors (Basel) 2019 Mar 1;9(1). Epub 2019 Mar 1.

Department of Biotechnology Engineering, Faculty of Engineering, International Islamic University Malaysia, Gombak 50728, Kuala Lumpur, Malaysia.

Performance of a sensing device is dependent on its construction material, especially for components that are directly involved in transporting and translating signals across the device. Understanding the morphology and characteristics of the material components is therefore crucial in the development of any sensing device. This work examines the morphological and electrochemical characteristics of reduced graphene oxide interspersed with poly(3,4-ethylenedioxythiophene):poly(styrenesulfonate) (rGO-PEDOT:PSS) used as a transducer material deposited on a commercially available screen-printed carbon electrode (SPCE). Read More

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http://dx.doi.org/10.3390/bios9010036DOI Listing
March 2019
3 Reads

Use of Electronic Noses for Diagnosis of Digestive and Respiratory Diseases through the Breath.

Biosensors (Basel) 2019 Feb 28;9(1). Epub 2019 Feb 28.

Industrial Engineering School, University of Extremadura, 06006 Badajoz, Spain.

The increased occurrence of chronic diseases related to lifestyle or environmental conditions may have a detrimental effect on long-term health if not diagnosed and controlled in time. For this reason, it is important to develop new noninvasive early diagnosis equipment that allows improvement of the current diagnostic methods. This, in turn, has led to an exponential development of technology applied to the medical sector, such as the electronic nose. Read More

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http://dx.doi.org/10.3390/bios9010035DOI Listing
February 2019
1 Read

Surface Enhanced Raman Spectroscopy of Lactoferrin Adsorbed on Silvered Porous Silicon Covered with Graphene.

Biosensors (Basel) 2019 Feb 28;9(1). Epub 2019 Feb 28.

Laboratory of Applied Plasmonics, Belarusian State University of Informatics and Radioelectronics, 220013 Minsk, Belarus.

We registered surface enhanced Raman scattering (SERS) spectra of the human lactoferrin molecules adsorbed on a silvered porous silicon (-Si) from 10⁻10 M solutions. It was found that the -Si template causes a negative surface potential of silver particles and their chemical resistivity to oxidation. These properties provided to attract positively charged lactoferrin molecules and prevent their interaction with metallic particles upon 473 nm laser excitation. Read More

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http://dx.doi.org/10.3390/bios9010034DOI Listing
February 2019
1 Read

In-Vitro Characterization of mCerulean3_mRuby3 as a Novel FRET Pair with Favorable Bleed-Through Characteristics.

Biosensors (Basel) 2019 Feb 28;9(1). Epub 2019 Feb 28.

Institute of Pharmacology and Toxicology, University of Zurich, 8057 Zurich, Switzerland.

In previous studies, we encountered substantial problems using the CFP_YFP Förster resonance energy transfer (FRET) pair to analyze protein proximity in the endoplasmic reticulum of live cells. Bleed-through of the donor emission into the FRET channel and overlap of the FRET emission wavelength with highly variable cellular autofluorescence significantly compromised the sensitivity of our analyses. Here, we propose mCerulean3 and mRuby3 as a new FRET pair to potentially overcome these problems. Read More

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http://dx.doi.org/10.3390/bios9010033DOI Listing
February 2019

Plasma from Patients with Rheumatoid Arthritis Reduces Nitric Oxide Synthesis and Induces Reactive Oxygen Species in A Cell-Based Biosensor.

Biosensors (Basel) 2019 Feb 27;9(1). Epub 2019 Feb 27.

Department of Clinical Biochemistry and Immunology, Faculty of Pharmacy, Universidad de Concepción, Victor Lamas 1290, Concepcion 4030000, Chile.

Rheumatoid arthritis (RA) has been associated with a higher risk of developing cardiovascular (CV) diseases. It has been proposed that systemic inflammation plays a key role in premature atherosclerosis development, and is therefore crucial to determine whether systemic components from RA patients promotes endothelial cell-oxidative stress by affecting reactive oxygen species (ROS) and nitric-oxide (NO) production. The aim of this study was to evaluate whether plasma from RA patients impair NO synthesis and ROS production by using the cell-line ECV-304 as a biosensor. Read More

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http://dx.doi.org/10.3390/bios9010032DOI Listing
February 2019
4 Reads

Electrochemical Sensor Based on Molecularly Imprinted Polymer for the Detection of Cefalexin.

Biosensors (Basel) 2019 Feb 27;9(1). Epub 2019 Feb 27.

Analytical Chemistry Department, Faculty of Pharmacy, Iuliu Hațieganu University of Medicine and Pharmacy, 4 Pasteur St., 400349 Cluj-Napoca, Romania.

In this study, a new electrochemical sensor was developed for the detection of cefalexin (CFX), based on the use of a molecularly imprinted polymer (MIP) obtained by electro‒polymerization in an aqueous medium of indole-3-acetic acid (I3AA) on a glassy carbon electrode (GCE) and on boron-doped diamond electrode (BDDE). The two different electrodes were used in order to assess how their structural differences and the difference in the potential applied during electrogeneration of the MIP translate to the performances of the MIP sensor. The quantification of CFX was performed by using the electrochemical signal of a redox probe before and after the rebinding of the template. Read More

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http://www.mdpi.com/2079-6374/9/1/31
Publisher Site
http://dx.doi.org/10.3390/bios9010031DOI Listing
February 2019
10 Reads

Dielectrophoresis Manipulation: Versatile Lateral and Vertical Mechanisms.

Biosensors (Basel) 2019 Feb 26;9(1). Epub 2019 Feb 26.

Institute of Microengineering and Nanoelectronics (IMEN), Universiti Kebangsaan Malaysia (UKM), Bangi, Selangor 43600, Malaysia.

Discussing the topic of the capability of dielectrophoresis (DEP) devices in terms of the selective detection and rapid manipulation of particles based on the DEP force (F) via contactless methods is challenging in medical research, drug discovery and delivery. Nonetheless, the process of the selective detection and rapid manipulation of particles via contactless DEP based on dielectric particles and the surrounding medium can reduce the effects of major issues, including physical contact with the particles and medium contamination to overcome operational difficulties. In this review, DEP microelectromechanical system (MEMS) microelectrodes with a tapered profile for the selective detection and rapid manipulation of particles were studied and compared with those of conventional designs with a straight-cut profile. Read More

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http://dx.doi.org/10.3390/bios9010030DOI Listing
February 2019

Differentiation of Patients with Balance Insufficiency (Vestibular Hypofunction) versus Normal Subjects Using a Low-Cost Small Wireless Wearable Gait Sensor.

Biosensors (Basel) 2019 Feb 26;9(1). Epub 2019 Feb 26.

Department of Otolaryngology, Texas Tech University Health Sciences Center, Lubbock, TX 79430, USA.

Balance disorders present a significant healthcare burden due to the potential for hospitalization or complications for the patient, especially among the elderly population when considering intangible losses such as quality of life, morbidities, and mortalities. This work is a continuation of our earlier works where we now examine feature extraction methodology on Dynamic Gait Index (DGI) tests and machine learning classifiers to differentiate patients with balance problems versus normal subjects on an expanded cohort of 60 patients. All data was obtained using our custom designed low-cost wireless gait analysis sensor (WGAS) containing a basic inertial measurement unit (IMU) worn by each subject during the DGI tests. Read More

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http://dx.doi.org/10.3390/bios9010029DOI Listing
February 2019

Response Surface Methodology for the Optimisation of Electrochemical Biosensors for Heavy Metals Detection.

Biosensors (Basel) 2019 Feb 13;9(1). Epub 2019 Feb 13.

Dipartimento di Scienze e Tecnologie Biologiche ed Ambientali, Via per Monteroni 1, 73100 Lecce, Italy.

Herein, we report the application of a chemometric tool for the optimisation of electrochemical biosensor performances. The experimental design was performed based on the responses of an amperometric biosensor developed for metal ions detection using the flow injection analysis. The electrode preparation and the working conditions were selected as experimental parameters, and thus, were modelled by a response surface methodology (RSM). Read More

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http://dx.doi.org/10.3390/bios9010026DOI Listing
February 2019

Toward Highly Efficient Cancer Imaging and Therapy Using the Environment-Friendly Chitosan Nanoparticles and NIR Laser.

Biosensors (Basel) 2019 Feb 18;9(1). Epub 2019 Feb 18.

Cancer Biology Department, Biochemistry and Molecular Biology Unit, National Cancer Institute, Cairo University, Giza 11796, Egypt.

Chitosan-tripolyphosphate nanoparticles (C-TPP NPs) were synthesized to investigate their cytotoxicity against colon cancer cells (Caco2 cells) in the absence and the presence of a near-infrared (NIR) laser to evaluate their influence in cancer detection using the NIR laser and to evaluate the NIR laser on cancer treatment. The synthesized NPs were characterized by Fourier transform infrared (FT-IR) spectroscopy, dynamic light scattering (DLS), zeta potential (ZP), and transmission electronic microscope (TEM). The cytotoxicity was analyzed by the MTT test and the cell viability was assessed using the Trypan blue method. Read More

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http://dx.doi.org/10.3390/bios9010028DOI Listing
February 2019
2 Reads

Saliva, a Magic Biofluid Available for Multilevel Assessment and a Mirror of General Health-A Systematic Review.

Biosensors (Basel) 2019 Feb 14;9(1). Epub 2019 Feb 14.

Iuliu Haţieganu" University of Medicine and Pharmacy Cluj-Napoca, Department of Analytical Chemistry, Faculty of Pharmacy, 400349 Cluj, Romania.

Background: Saliva has been recently proposed as an alternative to classic biofluid analyses due to both availability and reliability regarding the evaluation of various biomarkers. Biosensors have been designed for the assessment of a wide spectrum of compounds, aiding in the screening, diagnosis, and monitoring of pathologies and treatment efficiency. This literature review aims to present the development in the biosensors research and their utility using salivary assessment. Read More

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http://dx.doi.org/10.3390/bios9010027DOI Listing
February 2019

Development of Tin Oxide-Based Nanosensors for Electronic Nose Environmental Applications.

Biosensors (Basel) 2019 Feb 5;9(1). Epub 2019 Feb 5.

Institute of Physics Technology and Information (ITEFI-CSIC), 28006 Madrid, Spain.

Tin oxide nanofibres (NFs) are used as nanosensors in electronic noses. Their performance is compared to that of oxide commercial chemical sensors for pollutant detection. NFs were grown by electrospinning and deposited onto silicon substrates with integrated micro-hotplates. Read More

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http://dx.doi.org/10.3390/bios9010021DOI Listing
February 2019

The Recent Advances in Raman Microscopy and Imaging Techniques for Biosensors.

Biosensors (Basel) 2019 Feb 12;9(1). Epub 2019 Feb 12.

Thermo Fisher Scientific, 2 Radcliff Rd., Tewksbury, MA 01876, USA.

Raman microspectroscopy is now well established as one of the most powerful analytical techniques for a diverse range of applications in physical (material) and biological sciences. Consequently, the technique provides exceptional analytical opportunities to the science and technology of biosensing due to its capability to analyze both parts of a biosensor system-biologically sensitive components, and a variety of materials and systems used in physicochemical transducers. Recent technological developments in Raman spectral imaging have brought additional possibilities in two- and three-dimensional (2D and 3D) characterization of the biosensor's constituents and their changes on a submicrometer scale in a label-free, real-time nondestructive method of detection. Read More

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http://www.mdpi.com/2079-6374/9/1/25
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http://dx.doi.org/10.3390/bios9010025DOI Listing
February 2019
4 Reads

Rapid Drop-Test for Lectin Binding with Glycopolymer-Coated Optical Ring Resonators.

Biosensors (Basel) 2019 Feb 12;9(1). Epub 2019 Feb 12.

Fraunhofer Institute for Applied Polymer Research IAP, Geiselbergstr. 69, 14476 Potsdam, Germany.

We fabricated a simple sensor system for qualitative analysis of glycan-mediated interactions. Our main aim was to establish a ronbbust system that allowes drop-tests without complex fluidics. The test system should be usable in routine analytics in the future and bear sufficient sensitivity to detect binding events in the nanomolar range. Read More

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http://dx.doi.org/10.3390/bios9010024DOI Listing
February 2019
3 Reads

Fast identification of Bacteria for Quality Control of Drinking Water through A Static Headspace Sampler Coupled to a Sensory Perception System.

Biosensors (Basel) 2019 Feb 8;9(1). Epub 2019 Feb 8.

GISM group, Faculty of engineering and architecture, University of Pamplona, Pamplona⁻North of Santander, Colombia.

The aim of this study was to develop and implement a methodology composed by a Static Head-Space-Sampler (SHS) coupled to a Sensory Perception System (SPS) for the extraction of Volatile Organic Compounds (VOC's) emitted by bacterial species in the water. The SPS was performed by means of a chamber of 16 Metal-Oxide-Semiconductor (MOS) gas sensors and a software with pattern recognition methods for the detection and identification of bacteria. At first, the tests were conducted from the sterile and polluted water with the Escherichia coli bacteria and modifying the incubation temperatures (50 °C, 70 °C and 90 °C), with the objective to obtain an optimal temperature for the distinguishing of species. Read More

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http://www.mdpi.com/2079-6374/9/1/23
Publisher Site
http://dx.doi.org/10.3390/bios9010023DOI Listing
February 2019
5 Reads

SAM Composition and Electrode Roughness Affect Performance of a DNA Biosensor for Antibiotic Resistance.

Biosensors (Basel) 2019 Feb 7;9(1). Epub 2019 Feb 7.

Department of Biomedical Engineering, Wolfson Centre, 106 Rottenrow East, University of Strathclyde, Glasgow G1 1XQ, UK.

Antibiotic resistance is a growing concern in the treatment of infectious disease worldwide. Point-of-care (PoC) assays which rapidly identify antibiotic resistance in a sample will allow for immediate targeted therapy which improves patient outcomes and helps maintain the effectiveness of current antibiotic stockpiles. Electrochemical assays offer many benefits, but translation from a benchtop measurement system to low-cost portable electrodes can be challenging. Read More

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http://dx.doi.org/10.3390/bios9010022DOI Listing
February 2019
2 Reads

Trends and Perspectives in Immunosensors for Determination of Currently-Used Pesticides: The Case of Glyphosate, Organophosphates, and Neonicotinoids.

Biosensors (Basel) 2019 Feb 4;9(1). Epub 2019 Feb 4.

Dipartimento di Chimica, Università degli Studi di Firenze, Via della Lastruccia 3, 50019 Sesto Fiorentino (Fi), Italy.

Pesticides, due to their intensive use and their peculiar chemical features, can persist in the environment and enter the trophic chain, thus representing an environmental risk for the ecosystems and human health. Although there are several robust and reliable standard analytical techniques for their monitoring, the high frequency of contamination caused by pesticides requires methods for massive monitoring campaigns that are capable of rapidly detecting these compounds in many samples of different origin. Immunosensors represent a potential tool for simple, rapid, and sensitive monitoring of pesticides. Read More

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http://dx.doi.org/10.3390/bios9010020DOI Listing
February 2019
2 Reads

Integrated Microfluidic Devices Fabricated in Poly (Methyl Methacrylate) (PMMA) for On-site Therapeutic Drug Monitoring of Aminoglycosides in Whole Blood.

Biosensors (Basel) 2019 Jan 30;9(1). Epub 2019 Jan 30.

Australian Centre for Research on Separation Science (ACROSS), School of Natural Sciences-Chemistry, University of Tasmania, Private Bag 75, Hobart, Tasmania 7001, Australia.

On-site therapeutic drug monitoring (TDM) is important for providing a quick and accurate dosing to patients in order to improve efficacy and minimize toxicity. Aminoglycosides such as amikacin, gentamicin, and tobramycin are important antibiotics that have been commonly used to treat infections of chronic bacterial infections in the urinary tract, lung, and heart. However, these aminoglycosides can lead to vestibular and auditory dysfunction. Read More

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http://dx.doi.org/10.3390/bios9010019DOI Listing
January 2019
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A Molecular Interaction Analysis Reveals the Possible Roles of Graphene Oxide in a Glucose Biosensor.

Biosensors (Basel) 2019 Jan 28;9(1). Epub 2019 Jan 28.

Department of Physics, Bogor Agricultural University, Bogor 16680, Indonesia.

In this paper, we report the molecular docking study of graphene oxide and glucose oxidase (GOx) enzyme for a potential glucose biosensing application. The large surface area and good electrical properties have made graphene oxide as one of the best candidates for an enzyme immobilizer and transducer in the biosensing system. Our molecular docking results revealed that graphene oxide plays a role as a GOx enzyme immobilizer in the glucose biosensor system since it can spontaneously bind with GOx at specific regions separated from the active sites of glucose and not interfering or blocking the glucose sensing by GOx in an enzyme-assisted biosensor system. Read More

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http://www.mdpi.com/2079-6374/9/1/18
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http://dx.doi.org/10.3390/bios9010018DOI Listing
January 2019
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A Novel Wearable Device for Continuous Ambulatory ECG Recording: Proof of Concept and Assessment of Signal Quality.

Biosensors (Basel) 2019 Jan 21;9(1). Epub 2019 Jan 21.

Electrophysiology Division, Institut Universitaire de cardiologie et de pneumologie de Québec, Québec, G1V 4G5, QC, Canada.

Diagnosis of arrhythmic disorders is challenging because of their short-lasting, intermittent character. Conventional technologies of noninvasive ambulatory rhythm monitoring are limited by modest sensitivity. We present a novel form of wearable electrocardiogram (ECG) sensors providing an alternative tool for long-term rhythm monitoring with the potential of increased sensitivity to detect intermittent or subclinical arrhythmia. Read More

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http://www.mdpi.com/2079-6374/9/1/17
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http://dx.doi.org/10.3390/bios9010017DOI Listing
January 2019
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Ultrathin Functional Polymer Modified Graphene for Enhanced Enzymatic Electrochemical Sensing.

Biosensors (Basel) 2019 Jan 18;9(1). Epub 2019 Jan 18.

Systems and Process Engineering Centre (SPEC), Centre for NanoHealth, College of Engineering, Swansea University, Swansea, SA2 8PP, UK.

Grafting thin polymer layers on graphene enables coupling target biomolecules to graphene surfaces, especially through amide and aldehyde linkages with carboxylic acid and primary amine derivatives, respectively. However, functionalizing monolayer graphene with thin polymer layers without affecting their exceptional electrical properties remains challenging. Herein, we demonstrate the controlled modification of chemical vapor deposition (CVD) grown single layer graphene with ultrathin polymer 1,5-diaminonaphthalene (DAN) layers using the electropolymerization technique. Read More

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http://dx.doi.org/10.3390/bios9010016DOI Listing
January 2019
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Suppressing Non-Specific Binding of Proteins onto Electrode Surfaces in the Development of Electrochemical Immunosensors.

Biosensors (Basel) 2019 Jan 18;9(1). Epub 2019 Jan 18.

Tecnologico de Monterrey, Escuela de Ingeniería y Ciencias. Ave. Eugenio Garza Sada 2501, Monterrey 64849, N.L., Mexico.

Electrochemical immunosensors, EIs, are systems that combine the analytical power of electrochemical techniques and the high selectivity and specificity of antibodies in a solid phase immunoassay for target analyte. In EIs, the most used transducer platforms are screen printed electrodes, SPEs. Some characteristics of EIs are their low cost, portability for point of care testing (POCT) applications, high specificity and selectivity to the target molecule, low sample and reagent consumption and easy to use. Read More

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http://dx.doi.org/10.3390/bios9010015DOI Listing
January 2019

Textile-Based Potentiometric Electrochemical pH Sensor for Wearable Applications.

Biosensors (Basel) 2019 Jan 16;9(1). Epub 2019 Jan 16.

Bendable Electronics and Sensing Technology Group, School of Engineering, University of Glasgow, Glasgow G128QQ, UK.

In this work, we present a potentiometric pH sensor on textile substrate for wearable applications. The sensitive (thick film graphite composite) and reference electrodes (Ag/AgCl) are printed on cellulose-polyester blend cloth. An excellent adhesion between printed electrodes allow the textile-based sensor to be washed with a reliable pH response. Read More

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http://www.mdpi.com/2079-6374/9/1/14
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http://dx.doi.org/10.3390/bios9010014DOI Listing
January 2019
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Enzymatic Low Volume Passive Sweat Based Assays for Multi-Biomarker Detection.

Biosensors (Basel) 2019 Jan 16;9(1). Epub 2019 Jan 16.

Department of Bioengineering, University of Texas at Dallas, 800 West Campbell Road, Richardson, TX 75080, USA.

Simultaneous detection of correlated multi-biomarkers on a single low-cost platform in ultra-low fluid volumes with robustness is in growing demand for the development of wearable diagnostics. A non-faradaic biosensor for the simultaneous detection of alcohol, glucose, and lactate utilizing low volumes (1⁻5 μL) of sweat is demonstrated. Biosensing is implemented using nanotextured ZnO films integrated on a flexible porous membrane to achieve enhanced sensor performance. Read More

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http://dx.doi.org/10.3390/bios9010013DOI Listing
January 2019
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Biochemical Changes in Irradiated Oral Mucosa: A FTIR Spectroscopic Study.

Biosensors (Basel) 2019 Jan 13;9(1). Epub 2019 Jan 13.

SIB Labs, University of Eastern Finland, P.O. Box 1627, 70211 Kuopio, Finland.

Radiation exposure during the course of treatment in head and neck cancer (HNC) patients can induce both structural and biochemical anomalies. The present study is focused on utilizing infrared imaging for the identification of the minor biochemical alterations in the oral mucosa. Chemical maps generated using glycoprotein band indicates its differential distribution along the superficial layer. Read More

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http://www.mdpi.com/2079-6374/9/1/12
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http://dx.doi.org/10.3390/bios9010012DOI Listing
January 2019
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A Bioelectronic System to Measure the Glycolytic Metabolism of Activated CD4+ T Cells.

Biosensors (Basel) 2019 Jan 9;9(1). Epub 2019 Jan 9.

Life Sciences Discipline, Burnet Institute, Melbourne, VIC 3001, Australia.

The evaluation of glucose metabolic activity in immune cells is becoming an increasingly standard task in immunological research. In this study, we described a sensitive, inexpensive, and non-radioactive assay for the direct and rapid measurement of the metabolic activity of CD4+ T cells in culture. A portable, custom-built Cell Culture Metabolite Biosensor device was designed to measure the levels of acidification (a proxy for glycolysis) in cell-free CD4+ T cell culture media. Read More

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http://www.mdpi.com/2079-6374/9/1/10
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http://dx.doi.org/10.3390/bios9010010DOI Listing
January 2019
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Energy Transfer between Tm-Doped Upconverting Nanoparticles and a Small Organic Dye with Large Stokes Shift.

Biosensors (Basel) 2019 Jan 8;9(1). Epub 2019 Jan 8.

Department of Chemistry (Physical Chemistry), University of Potsdam, Karl-Liebknecht-Str. 24-25, 14476 Potsdam, Germany.

Lanthanide-doped upconverting nanoparticles (UCNP) are being extensively studied for bioapplications due to their unique photoluminescence properties and low toxicity. Interest in RET applications involving UCNP is also increasing, but due to factors such as large sizes, ion emission distributions within the particles, and complicated energy transfer processes within the UCNP, there are still many questions to be answered. In this study, four types of core and core-shell NaYF₄-based UCNP co-doped with Yb and Tm as sensitizer and activator, respectively, were investigated as donors for the Methyl 5-(8-decanoylbenzo[1,2-d:4,5-d']bis([1,3]dioxole)-4-yl)-5-oxopentanoate (DBD-6) dye. Read More

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http://www.mdpi.com/2079-6374/9/1/9
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http://dx.doi.org/10.3390/bios9010009DOI Listing
January 2019
8 Reads

Innovative Sensor Approach to Follow Development.

Biosensors (Basel) 2019 Jan 7;9(1). Epub 2019 Jan 7.

CNR-IBBR, Institute of Bioscience and Bioresources, via Madonna del Piano, 10, 50019 Sesto Fiorentino, FI, Italy.

infection affects more than 200,000 people every year in Europe and in the last four years a trend shows an increase in campylobacteriosis. The main vehicle for transmission of the bacterium is contaminated food like meat, milk, fruit and vegetables. In this study, the aim was to find characteristic volatile organic compounds (VOCs) of in order to detect its presence with an array of metal oxide (MOX) gas sensors. Read More

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http://www.mdpi.com/2079-6374/9/1/8
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http://dx.doi.org/10.3390/bios9010008DOI Listing
January 2019
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Characterization and Inkjet Printing of an RNA Aptamer for Paper-Based Biosensing of Ciprofloxacin.

Biosensors (Basel) 2019 Jan 2;9(1). Epub 2019 Jan 2.

Department of Biology, Technische Universität Darmstadt, 64287 Darmstadt, Germany.

The excessive use of antibiotics in food-producing animals causes a steady rise of multiple antibiotic resistance in foodborne bacteria. Next to sulfonamides, the most common antibiotics groups are fluoroquinolones, aminoglycosides, and ß-lactams. Therefore, there is a need for a quick, efficient, and low-cost detection procedure for antibiotics. Read More

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http://dx.doi.org/10.3390/bios9010007DOI Listing
January 2019
14 Reads

Real Time Monitoring of a UV Light-Assisted Biofunctionalization Protocol Using a Nanophotonic Biosensor.

Biosensors (Basel) 2018 Dec 30;9(1). Epub 2018 Dec 30.

Nanophotonics Technology Center, Universitat Politècnica de València, Camí de Vera s/n, 46022 Valencia, Spain.

A protocol for the covalent biofunctionalization of silicon-based biosensors using a UV light-induced thiol⁻ene coupling (TEC) reaction has been developed. This biofunctionalization approach has been used to immobilize half antibodies (hIgG), which have been obtained by means of a tris(2-carboxyethyl)phosphine (TCEP) reduction at the hinge region, to the surface of a vinyl-activated silicon-on-insulator (SOI) nanophotonic sensing chip. The response of the sensing structures within the nanophotonic chip was monitored in real time during the biofunctionalization process, which has allowed us to confirm that the bioconjugation of the thiol-terminated bioreceptors onto the vinyl-activated sensing surface is only initiated upon UV light photocatalysis. Read More

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http://dx.doi.org/10.3390/bios9010006DOI Listing
December 2018

Detection of Metabolic Changes Induced via Drug Treatments in Live Cancer Cells and Tissue Using Raman Imaging Microscopy.

Biosensors (Basel) 2018 Dec 28;9(1). Epub 2018 Dec 28.

Neuro-Oncology Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA.

Isocitrate dehydrogenase 1 (IDH1) mutations in gliomas, fibrosarcoma, and other cancers leads to a novel metabolite, D-2-hydroxyglutarate, which is proposed to cause tumorigenesis. The production of this metabolite also causes vulnerabilities in cellular metabolism, such as lowering NADPH levels. To exploit this vulnerability, we treated glioma and fibrosarcoma cells that harbor an IDH1 mutation with an inhibitor of nicotinamide adenine dinucleotide (NAD⁺) salvage pathway, FK866, and observed decreased viability in these cells. Read More

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http://dx.doi.org/10.3390/bios9010005DOI Listing
December 2018
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ZIF Nanocrystal-Based Surface Acoustic Wave (SAW) Electronic Nose to Detect Diabetes in Human Breath.

Biosensors (Basel) 2018 Dec 26;9(1). Epub 2018 Dec 26.

Instituto de Ciencias Aplicadas y Tecnología (ICAT), Universidad Nacional Autónoma de México, Ciudad Universitaria, Ciudad de México 04510, Mexico.

In the present work, a novel, portable and innovative eNose composed of a surface acoustic wave (SAW) sensor array based on zeolitic imidazolate frameworks, ZIF-8 and ZIF-67 nanocrystals (pure and combined with gold nanoparticles), as sensitive layers has been tested as a non-invasive system to detect different disease markers, such as acetone, ethanol and ammonia, related to the diagnosis and control of diabetes mellitus through exhaled breath. The sensors have been prepared by spin coating, achieving continuous sensitive layers at the surface of the SAW device. Low concentrations (5 ppm, 10 ppm and 25 ppm) of the marker analytes were measured, obtaining high sensitivities, good reproducibility, short time response and fast signal recovery. Read More

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http://dx.doi.org/10.3390/bios9010004DOI Listing
December 2018
1 Read