666 results match your criteria Biosensors[Journal]


Detection of Cardiovascular CRP Protein Biomarker Using a Novel Nanofibrous Substrate.

Biosensors (Basel) 2020 Jun 24;10(6). Epub 2020 Jun 24.

Department of Biomedical Engineering, University of Bridgeport, Bridgeport, CT 06604, USA.

It is known that different diseases have characteristic biomarkers that are secreted very early on, even before the symptoms have developed. Before any kind of therapeutic approach can be used, it is necessary that such biomarkers be detected at a minimum concentration in the bodily fluids. Here, we report the fabrication of an interdigitated sensing device integrated with polyvinyl alcohol (PVA) nanofibers and carbon nanotubes (CNT) for the detection of an inflammatory biomarker, C-reactive protein (CRP). Read More

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http://dx.doi.org/10.3390/bios10060072DOI Listing

A Capillary-Perfused, Nanocalorimeter Platform for Thermometric Enzyme-Linked Immunosorbent Assay with Femtomole Sensitivity.

Biosensors (Basel) 2020 Jun 24;10(6). Epub 2020 Jun 24.

Department of Biomedical Engineering, Vanderbilt University, Nashville, TN 37235, USA.

Enzyme-catalyzed chemical reactions produce heat. We developed an enclosed, capillary-perfused nanocalorimeter platform for thermometric enzyme-linked immunosorbent assay (TELISA). We used catalase as enzymes to model the thermal characteristics of the micromachined calorimeter. Read More

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http://dx.doi.org/10.3390/bios10060071DOI Listing

Laccase-Based Biosensor Encapsulated in a Galactomannan-Chitosan Composite for the Evaluation of Phenolic Compounds.

Biosensors (Basel) 2020 Jun 22;10(6). Epub 2020 Jun 22.

Institute of Analytical Sciences, University of Lyon, 69100 Villeurbanne, France.

Galactomannan, a neutral polysaccharide, was extracted from carob seeds and characterized. It was used for the first time for the fabrication of a laccase-based biosensor by the encapsulation of laccase in a chitosan+galactomannan composite. The fabricated biosensor was characterized by FTIR, scanning electron microscopy and cyclic voltammetry. Read More

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http://dx.doi.org/10.3390/bios10060070DOI Listing

A Novel Efficient FEM Thin Shell Model for Bio-Impedance Analysis.

Biosensors (Basel) 2020 Jun 17;10(6). Epub 2020 Jun 17.

School of Electrical and Electronics Engineering, The University of Manchester, Manchester M13 9PL, UK.

In this paper, a novel method for accelerating eddy currents calculation on a cell model using the finite element method (FEM) is presented. Due to the tiny thickness of cell membrane, a full-mesh cell model requires a large number of mesh elements and hence intensive computation resources and long time. In this paper, an acceleration method is proposed to reduce the number of mesh elements and therefore reduce the computing time. Read More

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http://dx.doi.org/10.3390/bios10060069DOI Listing

Plant Part-Derived Carbon Dots for Biosensing.

Biosensors (Basel) 2020 Jun 17;10(6). Epub 2020 Jun 17.

Department of Medicinal and Applied Chemistry, Kaohsiung Medical University, Kaohsiung 80708, Taiwan.

Carbon dots (CDs) are a new cluster of carbon atoms with particle size less than 10 nm. CDs also exhibit interesting fluorescence (FL) properties. CDs are attractive because of their fascinating characteristics including low toxicity, good water solubility, and tremendous biocompatibility. Read More

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http://dx.doi.org/10.3390/bios10060068DOI Listing

Recent Advances in Cellulose-Based Biosensors for Medical Diagnosis.

Biosensors (Basel) 2020 Jun 17;10(6). Epub 2020 Jun 17.

Dyeing, Printing and Auxiliaries Department, National Research Centre, Cairo 12622, Egypt.

Cellulose has attracted much interest, particularly in medical applications such as advanced biosensing devices. Cellulose could provide biosensors with enhanced biocompatibility, biodegradability and non-toxicity, which could be useful for biosensors. Thus, they play a significant role in environmental monitoring, medical diagnostic tools, forensic science, and foodstuff processing safety applications. Read More

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http://dx.doi.org/10.3390/bios10060067DOI Listing

Magnetic Bead-Based Electrochemical Immunoassays On-Drop and On-Chip for Procalcitonin Determination: Disposable Tools for Clinical Sepsis Diagnosis.

Biosensors (Basel) 2020 Jun 17;10(6). Epub 2020 Jun 17.

Department of Analytical Chemistry, Physical Chemistry and Chemical Engineering, Universidad de Alcalá, Ctra. Madrid-Barcelona, Km. 33.600, Alcalá de Henares, 28871 Madrid, Spain.

Procalcitonin (PCT) is a known protein biomarker clinically used for the early stages of sepsis diagnosis and therapy guidance. For its reliable determination, sandwich format magnetic bead-based immunoassays with two different electrochemical detection approaches are described: (i) disposable screen-printed carbon electrodes (SPE-C, on-drop detection); (ii) electro-kinetically driven microfluidic chips with integrated Au electrodes (EMC-Au, on-chip detection). Both approaches exhibited enough sensitivity (limit of detection (LOD) of 0. Read More

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http://dx.doi.org/10.3390/bios10060066DOI Listing

The Effect of Optically Induced Dielectrophoresis (ODEP)-Based Cell Manipulation in a Microfluidic System on the Properties of Biological Cells.

Biosensors (Basel) 2020 Jun 16;10(6). Epub 2020 Jun 16.

Division of Haematology/Oncology, Department of Internal Medicine, Chang Gung Memorial Hospital at Linkou, Taoyuan City 33302, Taiwan.

Cell manipulation using optically induced dielectrophoresis (ODEP) in microfluidic systems has attracted the interest of scientists due to its simplicity. Although this technique has been successfully demonstrated for various applications, one fundamental issue has to be addressed-Whether, the ODEP field affects the native properties of cells. To address this issue, we explored the effect of ODEP electrical conditions on cellular properties. Read More

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http://dx.doi.org/10.3390/bios10060065DOI Listing

Biosensor for Monitoring Blackleg and Soft Rot Disease of Potato.

Biosensors (Basel) 2020 Jun 15;10(6). Epub 2020 Jun 15.

Institute of Nanotechnology, CNR-Nanotec, Via per Monteroni, 73100 Lecce, Italy.

(Pba) is a quarantine and threatening phytopathogen known as the causal agent of blackleg and soft rot disease of potatoes in many areas. Its early detection is then important to have healthy potato tubers and reduce economic losses. Today, conventional methods such as enzyme-linked immunosorbent-assay (ELISA) and polymerase chain reaction (PCR) are typically used for Pba detection, but they are expensive and time-consuming. Read More

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http://dx.doi.org/10.3390/bios10060064DOI Listing

Low-Fouling Substrates for Plasmonic Sensing of Circulating Biomarkers in Biological Fluids.

Authors:
Elba Mauriz

Biosensors (Basel) 2020 Jun 10;10(6). Epub 2020 Jun 10.

Department of Nursing and Physiotherapy, Universidad de León, Campus de Vegazana, s/n, 24071 León, Spain.

The monitoring of biomarkers in body fluids provides valuable prognostic information regarding disease onset and progression. Most biosensing approaches use noninvasive screening tools and are conducted in order to improve early clinical diagnosis. However, biofouling of the sensing surface may disturb the quantification of circulating biomarkers in complex biological fluids. Read More

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http://dx.doi.org/10.3390/bios10060063DOI Listing

Study of the Dielectric Properties of Artificial Sweat Mixtures at Microwave Frequencies.

Biosensors (Basel) 2020 Jun 9;10(6). Epub 2020 Jun 9.

Schulich School of Engineering, University of Calgary, Calgary, AB T2N1N4, Canada.

Analysis of sweat is of interest for a variety of diagnosis and monitoring applications in healthcare. In this work, detailed measurements of the dielectric properties of solutions representing the major components of sweat are presented. The measurements include aqueous solutions of sodium chloride (NaCl), potassium chloride (KCl), urea, and lactic acid, as well as their mixtures. Read More

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http://dx.doi.org/10.3390/bios10060062DOI Listing

A Deformation of a Mercury Droplet under Acceleration in an Annular Groove.

Biosensors (Basel) 2020 Jun 9;10(6). Epub 2020 Jun 9.

School of Mechanical Engineering, University of Birmingham, Birmingham B15 2TT, UK.

Microelectromechanical system (MEMS) liquid sensors may be used under large acceleration conditions. It is important to understand the deformation of the liquid droplets under acceleration for the design and applications of MEMS liquid sensors, as this will affect the performance of the sensors. This paper presents an investigation into the deformation of a mercury droplet in a liquid MEMS sensor under accelerations and reports the relationship between the deformation and the accelerations. Read More

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http://dx.doi.org/10.3390/bios10060061DOI Listing

Smartphone and Paper-Based Fluorescence Reader: A Do It Yourself Approach.

Biosensors (Basel) 2020 Jun 2;10(6). Epub 2020 Jun 2.

Biophotonic Nanosensors Laboratory, Centro de Investigaciones en Óptica A. C., León 37150, Mexico.

Given their photoluminescent character, portable quantum dot readers are often sophisticated and relatively expensive. In response, we engineered a "do it yourself" fluorescence reader employing paper materials and a mid-range smartphone camera. Black paperboard facilitated a versatile, lightweight and foldable case; whereas cellophane paper was observed to behave as a simple, yet effective, optical bandpass filter leading to an advantageous device for the quantitative interrogation of quantum dot nanocrystals concentrations (from 2. Read More

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http://dx.doi.org/10.3390/bios10060060DOI Listing

Development and Evaluation of the Chromatic Behavior of an Intelligent Packaging Material Based on Cellulose Acetate Incorporated with Polydiacetylene for an Efficient Packaging.

Biosensors (Basel) 2020 May 31;10(6). Epub 2020 May 31.

Packaging Laboratory, Department of Food Technology, Universidade Federal de Viçosa, Viçosa-MG 36570-900, Brazil.

Global growth of the food industry and the demand for new products with natural characteristics, safe conditions and traceability have driven researches for the development of technologies such as intelligent packaging, capable to fulfil those needs. Polydiacetylene (PDA) is a synthetic material that has been highlighted in research field as a sensor substance, which can be used to produce intelligent packaging capable to detect chemical or biochemical changes in foods and in their environment due to PDA's color transition from blue to red. This work focused on the development and optimization of an intelligent packaging constituted of a polymeric matrix of cellulose acetate-based incorporated with PDA as the substance sensor. Read More

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http://dx.doi.org/10.3390/bios10060059DOI Listing

Application of Biosensors for Detection of Pathogenic Food Bacteria: A Review.

Biosensors (Basel) 2020 May 30;10(6). Epub 2020 May 30.

Food and Nutritional Science Program, North Carolina A & T State University, Greensboro, NC 27411, USA.

The use of biosensors is considered a novel approach for the rapid detection of foodborne pathogens in food products. Biosensors, which can convert biological, chemical, or biochemical signals into measurable electrical signals, are systems containing a biological detection material combined with a chemical or physical transducer. The objective of this review was to present the effectiveness of various forms of sensing technologies for the detection of foodborne pathogens in food products, as well as the criteria for industrial use of this technology. Read More

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http://dx.doi.org/10.3390/bios10060058DOI Listing

Characteristics of an Extended Gate Field-Effect Transistor for Glucose Sensing Using an Enzyme-Containing Silk Fibroin Membrane as the Bio-Chemical Component.

Biosensors (Basel) 2020 May 29;10(6). Epub 2020 May 29.

Nanomaterials Microdevices Research Center, Osaka Institute of Technology, Asahi-ku Ohmiya, Osaka 535-8585, Japan.

The characteristics of a glucose sensor based on an ion-sensitive TiO/Ti extended gate electrode field-effect transistor (EGFET) are reported. A glucose oxidase-containing silk fibroin membrane was immobilized on a TiO/Ti surface as the bio-sensing component. This EGFET-type biosensor was estimated to be able to detect a glucose concentration as low as 0. Read More

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http://dx.doi.org/10.3390/bios10060057DOI Listing

Wearable Skin Sensors and Their Challenges: A Review of Transdermal, Optical, and Mechanical Sensors.

Biosensors (Basel) 2020 May 28;10(6). Epub 2020 May 28.

Department of Chemical & Materials Engineering, University of Idaho, Moscow, ID 83844, USA.

Wearable technology and mobile healthcare systems are both increasingly popular solutions to traditional healthcare due to their ease of implementation and cost-effectiveness for remote health monitoring. Recent advances in research, especially the miniaturization of sensors, have significantly contributed to commercializing the wearable technology. Most of the traditional commercially available sensors are either mechanical or optical, but nowadays transdermal microneedles are also being used for micro-sensing such as continuous glucose monitoring. Read More

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http://dx.doi.org/10.3390/bios10060056DOI Listing

CO Laser Photoacoustic Spectrometer for Measuring Acetone in the Breath of Lung Cancer Patients.

Biosensors (Basel) 2020 May 27;10(6). Epub 2020 May 27.

Department of Physics, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia.

A CO laser has the advantages of being high in power and having many laser lines in the 9-11 µm infrared region. Thus, a CO laser photoacoustic spectrometer (PAS) can have a multi-component measurement capability for many gas compounds that have non-zero absorption coefficients at the laser lines, and therefore can be applied for measuring several volatile organic compounds (VOCs) in the human breath. We have developed a CO laser PAS system for detecting acetone in the human breath. Read More

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http://dx.doi.org/10.3390/bios10060055DOI Listing

Recent Progress on the Electrochemical Biosensing of O157:H7: Material and Methods Overview.

Biosensors (Basel) 2020 May 18;10(5). Epub 2020 May 18.

Physics and Electronics, Department of Sciences and Technology, Linköping University, SE-601 74 Norrköping, Sweden.

O157:H7 ( O157:H7) is a pathogenic strain of which has issued as a public health threat because of fatal contamination of food and water. Therefore, accurate detection of pathogenic is important in environmental and food quality monitoring. In spite of their advantages and high acceptance, culture-based methods, enzyme-linked immunosorbent assays (ELISAs), polymerase chain reaction (PCR), flow cytometry, ATP bioluminescence, and solid-phase cytometry have various drawbacks, including being time-consuming, requiring trained technicians and/or specific equipment, and producing biological waste. Read More

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http://dx.doi.org/10.3390/bios10050054DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7277213PMC

Silver SERS Adenine Sensors with a Very Low Detection Limit.

Biosensors (Basel) 2020 May 15;10(5). Epub 2020 May 15.

Department of Electrical Engineering, National Cheng Kung University, Tainan 701, Taiwan.

The detection of adenine molecules at very low concentrations is important for biological and medical research and applications. This paper reports a silver-based surface-enhanced Raman scattering (SERS) sensor with a very low detection limit for adenine molecules. Clusters of closely packed silver nanoparticles on surfaces of discrete ball-like copper bumps partially covered with graphene are deposited by immersion in silver nitrate. Read More

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http://dx.doi.org/10.3390/bios10050053DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7277772PMC

Paper-Based Working Electrodes Coated with Mercury or Bismuth Films for Heavy Metals Determination.

Biosensors (Basel) 2020 May 13;10(5). Epub 2020 May 13.

Departamento de Química Física y Analítica, Facultad de Química, Universidad de Oviedo, 33006 Oviedo, Spain.

Paper-based carbon working electrodes were modified with mercury or bismuth films for the determination of trace metals in aqueous solutions. Both modification procedures were optimized in terms of selectivity and sensitivity for the determination of different heavy metals, aiming their simultaneous determination. Cd (II), Pb (II) and In (III) could be quantified with both films. Read More

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http://dx.doi.org/10.3390/bios10050052DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7277893PMC

Yeast-Based Biosensors: Current Applications and New Developments.

Biosensors (Basel) 2020 May 13;10(5). Epub 2020 May 13.

Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement (INRAE), UMR 792 Toulouse Biotechnology Institute (TBI), 31400 Toulouse, France.

Biosensors are regarded as a powerful tool to detect and monitor environmental contaminants, toxins, and, more generally, organic or chemical markers of potential threats to human health. They are basically composed of a sensor part made up of either live cells or biological active molecules coupled to a transducer/reporter technological element. Whole-cells biosensors may be based on animal tissues, bacteria, or eukaryotic microorganisms such as yeasts and microalgae. Read More

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http://dx.doi.org/10.3390/bios10050051DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7277604PMC

Fecal Volatile Organic Compound Profiles are Not Influenced by Gestational Age and Mode of Delivery: A Longitudinal Multicenter Cohort Study.

Biosensors (Basel) 2020 May 11;10(5). Epub 2020 May 11.

Department of Pediatric Gastroenterology, Amsterdam UMC, Academic Medical Center, 1105 AZ Amsterdam, The Netherlands.

Fecal volatile organic compounds (VOC) reflect human and gut microbiota metabolic pathways and their interaction. VOC behold potential as non-invasive preclinical diagnostic biomarkers in various diseases, e.g. Read More

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http://dx.doi.org/10.3390/bios10050050DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7277672PMC

Battery-Powered Portable Rotary Real-Time Fluorescent qPCR with Low Energy Consumption, Low Cost, and High Throughput.

Biosensors (Basel) 2020 May 8;10(5). Epub 2020 May 8.

State Key Laboratory of Applied Optics, Changchun Institute of Optics, Fine Mechanics and Physics (CIOMP), Chinese Academy of Sciences, Changchun 130033, China.

The traditional qPCR instrument is bulky, expensive, and inconvenient to carry, so we report a portable rotary real-time fluorescent PCR (polymerase chain reaction) that completes the PCR amplification of DNA in the field, and the reaction can be observed in real-time. Through the analysis of a target gene, namely pGEM-3Zf (+), the gradient amplification and melting curves are compared to commercial devices. The results confirm the stability of our device. Read More

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http://dx.doi.org/10.3390/bios10050049DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7277348PMC

Smell - Adding a New Dimension to Urinalysis.

Biosensors (Basel) 2020 May 5;10(5). Epub 2020 May 5.

Department of Pediatric Nephrology, Emma Children's Hospital, Amsterdam UMC, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands.

Background: Urinary tract infections (UTI) are among the most common infections in children. The primary tool to detect UTI is dipstick urinalysis; however, this has limited sensitivity and specificity. Therefore, urine culture has to be performed to confirm a UTI. Read More

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http://dx.doi.org/10.3390/bios10050048DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7277101PMC

An Array of MOX Sensors and ANNs to Assess Grated Parmigiano Reggiano Cheese Packs' Compliance with CFPR Guidelines.

Biosensors (Basel) 2020 May 2;10(5). Epub 2020 May 2.

Department of Information Engineering, University of Brescia, Brescia, via Branze, 38, 25123 Brescia, BS, Italy.

Parmigiano Reggiano cheese is one of the most appreciated Italian foods on account of its high nutrient content and taste. Due to its high cost, these characteristics make this product subject to counterfeiting in different forms. In this study, an approach based on an array of gas sensors has been employed to assess if it was possible to distinguish different samples based on their aroma. Read More

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http://dx.doi.org/10.3390/bios10050047DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7277510PMC

On the Electrochemical Detection of Alpha-Fetoprotein Using Aptamers: DNA Isothermal Amplification Strategies to Improve the Performance of Weak Aptamers.

Biosensors (Basel) 2020 Apr 30;10(5). Epub 2020 Apr 30.

Departamento de Química Física y Analítica, Universidad de Oviedo, Av. Julián Clavería 8, 33006 Oviedo, Spain.

Affinity characterization is essential to develop reliable aptamers for tumor biomarker detection. For alpha-fetoprotein (AFP), a biomarker of hepatocellular carcinoma (HCC), two DNA aptamers were described with very different affinity. In this work, we estimate the dissociation constant of both of them by means of a direct assay on magnetic beads modified with AFP and electrochemical detection on carbon screen-printed electrodes (SPCE). Read More

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http://dx.doi.org/10.3390/bios10050046DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7277757PMC

Immobilization Techniques for Aptamers on Gold Electrodes for the Electrochemical Detection of Proteins: A Review.

Biosensors (Basel) 2020 Apr 28;10(5). Epub 2020 Apr 28.

Institute for Bioprocessing and Analytical Measurement Techniques, 37308 Heilbad Heiligenstadt, Germany.

The development of reliable biosensing platforms plays a key role in the detection of proteins in clinically and environmentally derived samples for diagnostics, as well as for process monitoring in biotechnological productions. For this purpose, the biosensor has to be stable and reproducible, and highly sensitive to detect potentially extremely low concentrations and prevent the nonspecific binding of interfering compounds. In this review, we present an overview of recently published (2017-2019) immobilization techniques for aptamers on gold electrodes for the electrochemical detection of proteins. Read More

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http://dx.doi.org/10.3390/bios10050045DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7277302PMC

Host-Modulation Therapy and Chair-Side Diagnostics in the Treatment of Peri-Implantitis.

Biosensors (Basel) 2020 Apr 25;10(5). Epub 2020 Apr 25.

Department of Oral and Maxillofacial Diseases, Head and Neck Center, University of Helsinki and Helsinki University Hospital, PO Box 63 (Haartmaninkatu 8) FI-00014 Helsinki, Finland.

Previous studies report periodontitis and peri-implantitis being able to induce systemic low-grade inflammation, which is known to be associated with increased risk for some systemic medical disease such as cardiovascular disease. In this regard, recent studies have shown that host modulation therapy (HMT) together with traditional mechanical and surgical treatment not only cease the progression of periodontitis but also reduce the systemic collagenolytic biomarkers in both oral fluids and circulation. This suggests that the corresponding adjunctive HMT-medication could be effective in the prevention and treatment of dental peri-implantitis, as well. Read More

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http://dx.doi.org/10.3390/bios10050044DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7277891PMC

Design and Fabrication of Capillary-Driven Flow Device for Point-Of-Care Diagnostics.

Biosensors (Basel) 2020 Apr 15;10(4). Epub 2020 Apr 15.

Bioengineering Research Group, Faculty of Engineering and Physical Sciences, University of Southampton, Southampton SO17 1BJ, UK.

Point-of-care (POC) diagnostics enables the diagnosis and monitoring of patients from the clinic or their home. Ideally, POC devices should be compact, portable and operatable without the requirement of expertise or complex fluid mechanical controls. This paper showcases a chip-and-dip device, which works on the principle of capillary-driven flow microfluidics and allows analytes' detection by multiple microchannels in a single microchip via smartphone imaging. Read More

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http://dx.doi.org/10.3390/bios10040039DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7235737PMC

Development of Novel Magneto-Biosensor for Sulfapyridine Detection.

Biosensors (Basel) 2020 Apr 21;10(4). Epub 2020 Apr 21.

Univ Lyon, CNRS, University Claude Bernard Lyon 1, Institut des Sciences Analytiques, UMR 5280, 5 rue de la Doua, F-69100 Villeurbanne, France.

In this work, we report the development of a highly sensitive biosensor for sulfapyridine detection based on an integrated bio micro-electromechanical system (Bio-MEMS) containing four gold working electrodes (WEs), a platinum counter electrode (CE), and a reference electrode (RE). Firstly, the cleaned WEs were modified with 4-aminophenylacetic acid (CMA). Then, (5-[4-(amino)phenylsulfonamide]-5-oxopentanoic acid (SA2BSA) was immobilized onto the transducers surface by carbodiimide chemistry. Read More

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http://dx.doi.org/10.3390/bios10040043DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7235803PMC

Advances in Sensing Technologies for Monitoring of Bone Health.

Biosensors (Basel) 2020 Apr 21;10(4). Epub 2020 Apr 21.

Graduate School of Biomedical Engineering, Faculty of Engineering, The University of New South Wales, Sydney, NSW 2052, Australia.

Changing lifestyle and food habits are responsible for health problems, especially those related to bone in an aging population. Poor bone health has now become a serious matter of concern for many of us. In order to avoid serious consequences, the early prediction of symptoms and diagnosis of bone diseases have become the need of the hour. Read More

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http://dx.doi.org/10.3390/bios10040042DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7235906PMC

Rapid Nondestructive Detection of Water Content and Granulation in Postharvest "Shatian" Pomelo Using Visible/Near-Infrared Spectroscopy.

Biosensors (Basel) 2020 Apr 20;10(4). Epub 2020 Apr 20.

Public Monitoring Center for Agro-Product of Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China.

Visible/near-infrared (VIS/NIR) spectroscopy is a powerful tool for rapid, nondestructive fruit quality detection. This technology has been widely applied for quality detection of small, thin-peeled fruit, though less so for large, thick-peeled fruit due to a weak spectral signal resulting in a reduction of accuracy. More modeling work should be focused on solving this problem. Read More

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http://dx.doi.org/10.3390/bios10040041DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7235785PMC

Identification of Suitable Biomarkers for Stress and Emotion Detection for Future Personal Affective Wearable Sensors.

Biosensors (Basel) 2020 Apr 16;10(4). Epub 2020 Apr 16.

Biomedical Sciences and Biomedical Engineering, The University of Reading, Reading RG6 6AY, UK.

Skin conductivity (i.e., sweat) forms the basis of many physiology-based emotion and stress detection systems. Read More

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http://dx.doi.org/10.3390/bios10040040DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7235866PMC

Electrochemical Immunosensor for Human IgE Using Ferrocene Self-Assembled Monolayers Modified ITO Electrode.

Biosensors (Basel) 2020 Apr 14;10(4). Epub 2020 Apr 14.

Department of Chemistry, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Korea.

The immunoglobulin E (IgE) level in serum is an important factor in the examination of allergy. Ferrocene (Fc)-modified self-assembled monolayers (SAMs) were placed on an indium tin oxide (ITO) electrode as a sensing layer for the detection of human IgE. The Fc moiety in the SAMs facilitated the electron transfer through the organic SAMs layer and electrocatalytic signal amplification. Read More

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http://dx.doi.org/10.3390/bios10040038DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7235775PMC

Integrated Electrochemical Biosensors for Detection of Waterborne Pathogens in Low-Resource Settings.

Biosensors (Basel) 2020 Apr 13;10(4). Epub 2020 Apr 13.

Centre for Biosensors, Bioelectronics and Biodevices (C3Bio) and Department of Electronic and Electrical Engineering, University of Bath, Bath BA2 7AY, UK.

More than 783 million people worldwide are currently without access to clean and safe water. Approximately 1 in 5 cases of mortality due to waterborne diseases involve children, and over 1.5 million cases of waterborne disease occur every year. Read More

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http://dx.doi.org/10.3390/bios10040036DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7236604PMC

Instrumenting (Paddlefish) Rostra in Flowing Water with Strain Gages and Accelerometers.

Biosensors (Basel) 2020 Apr 11;10(4). Epub 2020 Apr 11.

US Army Engineer Research & Development Center, Environmental Lab, 3909 Halls Ferry Road, Vicksburg, MS 39180, USA.

The prominent rostrum of the North American Paddlefish, supported by a lattice-like endoskeleton, is highly durable, making it an important candidate for bio-inspiration studies. Energy dissipation and load-bearing capacity of the structure from extreme physical force has been demonstrated superior to that of man-made systems, but response to continuous hydraulic forces is unknown and requires special instrumentation for in vivo testing on a live fish. A single supply strain gage amplifier circuit has been combined with a digital three-axis accelerometer, implemented in a printed circuit board (PCB), and integrated with the commercial-off-the-shelf Adafruit Feather M0 datalogger with a microSD card. Read More

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http://dx.doi.org/10.3390/bios10040037DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7236600PMC

Flexible and Printed Electrochemical Immunosensor Coated with Oxygen Plasma Treated SWCNTs for Histamine Detection.

Biosensors (Basel) 2020 Apr 10;10(4). Epub 2020 Apr 10.

Faculty of Science and Technology, Free University of Bolzano-Bozen, 39100 Bolzano, Italy.

Heterocyclic amine histamine is a well-known foodborne toxicant (mostly linked to "scombroid poisoning") synthesized from the microbial decarboxylation of amino acid histidine. In this work, we report the fabrication of a flexible screen-printed immunosensor based on a silver electrode coated with single-walled carbon nanotubes (SWCNTs) for the detection of histamine directly in fish samples. Biosensors were realized by first spray depositing SWCNTs on the working electrodes and by subsequently treating them with oxygen plasma to reduce the unwanted effects related to their hydrophobicity. Read More

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http://dx.doi.org/10.3390/bios10040035DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7235776PMC

Investigation of Photoplethysmography Behind the Ear for Pulse Oximetry in Hypoxic Conditions with a Novel Device (SPYDR).

Biosensors (Basel) 2020 Apr 4;10(4). Epub 2020 Apr 4.

Spotlight Labs, 13335 15 Mile Rd Ste-135, Sterling Heights, MI 48312, USA.

Photoplethysmography (PPG) is a valuable technique for noninvasively evaluating physiological parameters. However, traditional PPG devices have significant limitations in high-motion and low-perfusion environments. To overcome these limitations, we investigated the accuracy of a clinically novel PPG site using SPYDR, a new PPG sensor suite, against arterial blood gas (ABG) measurements as well as other commercial PPG sensors at the finger and forehead in hypoxic environments. Read More

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http://dx.doi.org/10.3390/bios10040034DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7235881PMC

Electrochemical Sensing of Serotonin by a Modified MnO-Graphene Electrode.

Biosensors (Basel) 2020 Apr 2;10(4). Epub 2020 Apr 2.

Department of Engineering, University of Messina, 98166 Messina, Italy.

The development of MnO-graphene (MnOGR) composite by microwave irradiation method and its application as an electrode material for the selective determination of serotonin (SE), popularly known as "happy chemical", is reported. Anchoring MnO nanoparticles on graphene, yielded MnO-GR composite with a large surface area, improved electron transport, high conductivity and numerous channels for rapid diffusion of electrolyte ions. The composite was characterized by X-ray photoelectron spectroscopy (XPS), Raman spectroscopy and scanning electron microscopy (SEM) for assessing the actual composition, structure and morphology. Read More

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http://dx.doi.org/10.3390/bios10040033DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7235847PMC

Electrochemical (Bio)Sensors for Pesticides Detection Using Screen-Printed Electrodes.

Biosensors (Basel) 2020 Apr 2;10(4). Epub 2020 Apr 2.

NanoBioAnalysis Group-Department of Physical and Analytical Chemistry, University of Oviedo, Julián Clavería 8, 33006 Oviedo, Spain.

Pesticides are among the most important contaminants in food, leading to important global health problems. While conventional techniques such as high-performance liquid chromatography (HPLC) and mass spectrometry (MS) have traditionally been utilized for the detection of such food contaminants, they are relatively expensive, time-consuming and labor intensive, limiting their use for point-of-care (POC) applications. Electrochemical (bio)sensors are emerging devices meeting such expectations, since they represent reliable, simple, cheap, portable, selective and easy to use analytical tools that can be used outside the laboratories by non-specialized personnel. Read More

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http://dx.doi.org/10.3390/bios10040032DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7236603PMC

AC and DC Differential Bridge Structure Suitable for Electrochemical Interfacial Capacitance Biosensing Applications.

Biosensors (Basel) 2020 Mar 22;10(3). Epub 2020 Mar 22.

Electrical Engineering Department, Iowa State University, Ames, IA 50010, USA.

This paper presents a capacitive differential bridge structure with both AC and DC excitation and balancing capability for low cost electrode-solution interfacial capacitance biosensing applications. The proposed series RC balancing structure offers higher sensitivity, lower susceptibility to common-mode interferences, and drift control. To evaluate the bridge performance in practice, possible effects of initial bridge imbalance due to component mismatches are investigated considering the required resolution of the balancing networks, sensitivity, and linearity. Read More

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http://dx.doi.org/10.3390/bios10030028DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7146243PMC

Competitive HRP-Linked Colorimetric Aptasensor for the Detection of Fumonisin B1 in Food based on Dual Biotin-Streptavidin Interaction.

Biosensors (Basel) 2020 Mar 30;10(4). Epub 2020 Mar 30.

State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China.

Fumonisin B1 (FB1) is the most prevalent and toxic form among fumonisin homologues which are produced by fusarium species and it contaminates various types of food products, posing serious health hazards for humans and animals. In this work, a colorimetric assay for the detection of FB1 has been developed based on competitive horseradish peroxidase (HRP)-linked aptamer and dual biotin-streptavidin interaction. In short, a biotinylated aptamer of FB1 was immobilized on the microplate by biotin-streptavidin binding; the complementary strand (csDNA) of the aptamer was ligated with HRP by biotin-streptavidin binding again to form a csDNA-HRP sensing probe, competing with FB1 to bind to the aptamer. Read More

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http://dx.doi.org/10.3390/bios10040031DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7235773PMC

Graphene Oxide Nanoribbons in Chitosan for Simultaneous Electrochemical Detection of Guanine, Adenine, Thymine and Cytosine.

Biosensors (Basel) 2020 Mar 27;10(4). Epub 2020 Mar 27.

Department of Physical and Environmental Sciences, University of Toronto Scarborough, 1265 Military Trail, Toronto, ON M1C 1A4, Canada.

Herein, graphene oxide nanoribbons (GONRs) were obtained from the oxidative unzipping of multi-walled carbon nanotubes. Covalent coupling reaction of 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) and N-hydroxy succinimide (NHS) with amine functional groups (-NH) of the chitosan natural polymer (CH) was used for entrapping GONRs on the activated glassy carbon electrode (GCE/GONRs-CH). The nanocomposite was characterized by high-resolution transmission electron microscopy (HRTEM), and field-emission scanning electron microscopy (FESEM). Read More

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http://dx.doi.org/10.3390/bios10040030DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7236021PMC

Spectroscopic and Structural Analysis of Cu-Induced Fluorescence Quenching of ZsYellow.

Biosensors (Basel) 2020 Mar 23;10(3). Epub 2020 Mar 23.

Department of Life Science, Pohang University of Science and Technology, Pohang 37673, Korea.

Fluorescent proteins exhibit fluorescence quenching by specific transition metals, suggesting their potential as fluorescent protein-based metal biosensors. Each fluorescent protein exhibits unique spectroscopic properties and mechanisms for fluorescence quenching by metals. Therefore, the metal-induced fluorescence quenching analysis of various new fluorescent proteins would be important step towards the development of such fluorescent protein-based metal biosensors. Read More

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http://dx.doi.org/10.3390/bios10030029DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7146301PMC

Graphene Oxide Bulk-Modified Screen-Printed Electrodes Provide Beneficial Electroanalytical Sensing Capabilities.

Biosensors (Basel) 2020 Mar 19;10(3). Epub 2020 Mar 19.

Faculty of Science and Engineering, Manchester Metropolitan University, Chester Street, Manchester M1 5GD, UK.

We demonstrate a facile methodology for the mass production of graphene oxide (GO) bulk-modified screen-printed electrodes (GO-SPEs) that are economical, highly reproducible and provide analytically useful outputs. Through fabricating GO-SPEs with varying percentage mass incorporations (2.5%, 5%, 7. Read More

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http://dx.doi.org/10.3390/bios10030027DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7146522PMC

The Emergence of Insect Odorant Receptor-Based Biosensors.

Biosensors (Basel) 2020 Mar 17;10(3). Epub 2020 Mar 17.

Institute of Agricultural Engineering, Agricultural Research Organization, Volcani Center, Rishon LeZion 5025001, Israel.

The olfactory receptor neurons of insects and vertebrates are gated by odorant receptor (OR) proteins of which several members have been shown to exhibit remarkable sensitivity and selectivity towards volatile organic compounds of significant importance in the fields of medicine, agriculture and public health. Insect ORs offer intrinsic amplification where a single binding event is transduced into a measurable ionic current. Consequently, insect ORs have great potential as biorecognition elements in many sensor configurations. Read More

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http://dx.doi.org/10.3390/bios10030026DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7146604PMC

Recyclable SERS-Based Immunoassay Guided by Photocatalytic Performance of FeO@TiO@Au Nanocomposites.

Biosensors (Basel) 2020 Mar 16;10(3). Epub 2020 Mar 16.

Department of Microelectronic Science and Engineering, School of Physical Science and Technology, Ningbo University, Ningbo 315211, China.

A novel recyclable surface-enhanced Raman scattering (SERS)-based immunoassay was demonstrated and exhibited extremely high sensitivity toward prostate specific antigen (PSA). The immunoassay, which possessed a sandwich structure, was constructed of multifunctional FeO@TiO@Au nanocomposites as immune probe and Ag-coated sandpaper as immune substrate. First, by adjusting the density of outside Au seeds on FeO@TiO core-shell nanoparticles (NPs), the structure-dependent SERS and photocatalytic performance of the samples was explored by monitoring and degradating 4-mercaptobenzonic acid (4MBA). Read More

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http://dx.doi.org/10.3390/bios10030025DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7146594PMC

A Label-Free Immunosensor Based on Graphene Oxide/FeO/Prussian Blue Nanocomposites for the Electrochemical Determination of HBsAg.

Biosensors (Basel) 2020 Mar 14;10(3). Epub 2020 Mar 14.

College of Electronic Engineering and Automation, Guilin University of Electronic Technology, Guilin 541004, China.

In this article, a highly sensitive label-free immunosensor based on a graphene oxide (GO)/FeO/Prussian blue (PB) nanocomposite modified electrode was developed for the determination of human hepatitis B surface antigen (HBsAg). In this electrochemical immunoassay system, PB was used as a redox probe, while GO/FeO/PB nanocomposites and AuNPs were prepared and coated on screen-printed electrodes to enhance the detection sensitivity and to immobilize the hepatitis B surface antibody (HBsAb). The immunosensor was fabricated based on the principle that the decrease in peak currents of PB is proportional to the concentration of HBsAg captured on the modified immunosensor. Read More

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http://dx.doi.org/10.3390/bios10030024DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7146221PMC

Simultaneous Determination of Streptomycin and Oxytetracycline using a Oracet-Blue/Silver-Nanoparticle/Graphene-Oxide/Modified Screen-Printed Electrode.

Biosensors (Basel) 2020 Mar 11;10(3). Epub 2020 Mar 11.

Department of Chemistry, University of Toronto, 80 St. George Street, Toronto, ON M5S 3H6, Canada.

In this paper, an electrochemical technique is introduced for the determination of streptomycin (STR) in the presence of oxytetracycline (OTC) in milk samples. A novel bifunctional modified screen-printed electrode (SPE) modified with oracet blue, silver nanoparticles, and graphene oxide (OB/SNPs/GO/SPE) was fabricated. The modified electrode plays a catalyzer role for electrooxidation of STR at pH = 7. Read More

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http://dx.doi.org/10.3390/bios10030023DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7146413PMC