12,056 results match your criteria Biosens Bioelectron[Journal]


An electrochemical immunosensor using gold nanoparticles-PAMAM-nanostructured screen-printed carbon electrodes for tau protein determination in plasma and brain tissues from Alzheimer patients.

Biosens Bioelectron 2020 Sep 5;163:112238. Epub 2020 May 5.

Department of Analytical Chemistry, Faculty of Chemical Sciences, Complutense University of Madrid, Madrid, 28040, Spain. Electronic address:

This work reports a new sensitive strategy for the determination of tau protein, a hallmark of Alzheimer's disease (AD), involving a sandwich immunoassay and amperometric detection at disposable screen-printed carbon electrodes (SPCEs) modified with a gold nanoparticles-poly(amidoamine) (PAMAM) dendrimer nanocomposite (3D-Au-PAMAM) covalently immobilized onto electrografted p-aminobenzoic acid (p-ABA). The capture antibody (CAb) was immobilized by crosslinking with glutaraldehyde (GA) on the amino groups of the 3D-Au-PAMAM-p-ABA-SPCE, where tau protein was sandwiched with a secondary antibody labeled with horseradish peroxidase (HRP-DAb). Amperometry at -200 mV (vs the Ag pseudo-reference electrode) upon the addition of hydroquinone (HQ) as electron transfer mediator and HO as the enzyme substrate was used to detect the immunocomplex formation. Read More

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http://dx.doi.org/10.1016/j.bios.2020.112238DOI Listing
September 2020

In-particle stem-loop RT-qPCR for specific and multiplex microRNA profiling.

Biosens Bioelectron 2020 Sep 18;163:112301. Epub 2020 May 18.

Center for Molecular Recognition Research, Materials and Life Science Research Division, Korea Institute of Science and Technology, Seoul, 02792, Republic of Korea; Biomedical Engineering, University of Science and Technology (UST), Daejeon, 34113, Republic of Korea. Electronic address:

Here we report a novel method of microRNA (miRNA) profiling with particle-based multiplex quantitative reverse transcription polymerase chain reaction (RT-qPCR). To achieve target-specific reaction in a particle, the stem-loop RT primer and forward primer for each target miRNA were chemically immobilized to the particle. Target-specific cDNA synthesis proceeds with the stem-loop RT primer and then qPCR subsequently proceeds with the forward primer to rapidly achieve a quantitative result. Read More

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http://dx.doi.org/10.1016/j.bios.2020.112301DOI Listing
September 2020

Quantitative hematocrit measurement of whole blood in a point-of-care lateral flow device using a smartphone flow tracking app.

Biosens Bioelectron 2020 Sep 18;163:112300. Epub 2020 May 18.

Nanoelectronics Laboratory, Department of Electrical Engineering and Computer Science, University of Cincinnati, Cincinnati, OH, 45221-0030, USA. Electronic address:

We present a rapid and quantitative point-of-care (PoC) system based on a smartphone application that is capable of accurately tracking the flow of red blood cells (RBCs) through a no-reaction lateral flow assay (nrLFA) device. Utilizing only the camera feed from the smartphone and built-in image processing, the nrLFA is identified and RBC fluid flow distances and rates are recorded in parallel with the test without the need of any custom hardware or enclosure. We demonstrated the application by first measuring and then calculating hematocrit (Hct) values of whole blood samples with nominal content of 28%, 35%, 40%, and 45% Hct on the nrLFA platform. Read More

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http://dx.doi.org/10.1016/j.bios.2020.112300DOI Listing
September 2020

Electrospray deposition as a smart technique for laccase immobilisation on carbon black-nanomodified screen-printed electrodes.

Biosens Bioelectron 2020 Sep 16;163:112299. Epub 2020 May 16.

Institute of Crystallography, National Research Council, Area Della Ricerca di Roma 1, Via Salaria Km 29.300, 00015, Monterotondo, Italy. Electronic address:

Enzymes immobilisation represents a critical issue in the design of biosensors to achieve standardization as well as suitable analytical performances in terms of sensitivity, selectivity, and stability. In this work electrospray deposition (ESD) has been exploited as a novel technique for the immobilisation of laccase enzyme on carbon black modified screen-printed electrodes. The aim is to fabricate an amperometric biosensor for phenolic compound detection. Read More

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http://dx.doi.org/10.1016/j.bios.2020.112299DOI Listing
September 2020

An integrative microfluidic device for isolation and ultrasensitive detection of lung cancer-specific exosomes from patient urine.

Biosens Bioelectron 2020 Sep 18;163:112290. Epub 2020 May 18.

School of Ophthalmology & Optometry, School of Biomedical Engineering, Wenzhou Medical University, Wenzhou, Zhejiang, 325035, China; Wenzhou Institute, University of Chinese Academy of Sciences, Wenzhou, Zhejiang, 325001, China; The First Affiliated Hospital, Department of Preventive Medicine, Wenzhou Medical University, Wenzhou, Zhejiang, 325035, China. Electronic address:

Exosomes, derived from various biofluids, may serve as potential biomarkers for cancer early detection. Nevertheless, exosome clinical translation remains challenging due to the lack of reliable isolation and detection methods. Herein, we present a novel integrated microfluidic device specifically designed for isolation and in-situ detection of lung cancer-specific exosomes collected from patient's urine. Read More

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http://dx.doi.org/10.1016/j.bios.2020.112290DOI Listing
September 2020

Wearable breath monitoring via a hot-film/calorimetric airflow sensing system.

Biosens Bioelectron 2020 Sep 18;163:112288. Epub 2020 May 18.

Sensors and Microsystems Laboratory, Tsinghua-Berkeley Shenzhen Institute, Tsinghua University, Shenzhen, 518055, China; Berkeley Sensor & Actuator Center, University of California Berkeley, Berkeley, CA, 94720, USA; Mechanical Engineering Department, University of California Berkeley, Berkeley, CA, 94720, USA. Electronic address:

Monitoring the breath information from two nostrils can detect breath-related health problems. In this work, we introduce a wearable hot-film/calorimetric breath sensing system composed of a hot-film senor in the center and two calorimetric sensors on two sides. This design has the advantages of low power consumption of 60 mW and good sensitivity to simultaneously measure the mix breath velocity and individual breath airflow signals from the two nostrils. Read More

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http://dx.doi.org/10.1016/j.bios.2020.112288DOI Listing
September 2020
6.409 Impact Factor

Nanochannel-driven rapid capture of sub-nanogram level biomarkers for painless preeclampsia diagnosis.

Biosens Bioelectron 2020 Sep 11;163:112281. Epub 2020 May 11.

Department of Biomaterials Science, Pusan National University, Miryang, 50463, Republic of Korea. Electronic address:

Preeclampsia (PE) is a pregnancy-specific hypertensive syndrome recognized as the leading cause of maternal and fetal morbidity and mortality worldwide. Painful blood-collection procedures or low accuracy of non-invasive approaches require faster, patient-friendly, and more sensitive diagnostic technologies. Here we report a painless, highly sensitive detection platform using nanoporous microneedles (nMNs) that enables rapid capture of biomarkers present at sub-nanogram levels. Read More

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http://dx.doi.org/10.1016/j.bios.2020.112281DOI Listing
September 2020

Terminal deoxynucleotidyl transferase induced activators to unlock the trans-cleavage of CRISPR/Cpf 1 (TdT-IU- CRISPR/Cpf 1): An ultrasensitive biosensor for Dam MTase activity detection.

Biosens Bioelectron 2020 Sep 19;163:112271. Epub 2020 May 19.

Key Laboratory for Biorheological Science and Technology of Ministry of Education, State and Local Joint Engineering Laboratory for Vascular Implants, Bioengineering College of Chongqing University, Chongqing 400044, PR China. Electronic address:

DNA adenine methylation methyltransferase (Dam MTase) plays an important role in gene expression and cell development, and involves in some development of tumors. There are many methods to detect Dam MTase activity, yet they have some shortcomings such as high cost, limited detection limit and complicated operation. Here, a new fast and simple multiple amplification strategy based on terminal deoxynucleotidyl transferase induced activators to unlock the collateral cleavage activities (trans-cleavage) of CRISPR/Cpf 1 (TdT-IU-CRISPR/Cpf 1) was firstly established. Read More

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http://dx.doi.org/10.1016/j.bios.2020.112271DOI Listing
September 2020

Electrochemical (bio) sensors go green.

Biosens Bioelectron 2020 Sep 15;163:112270. Epub 2020 May 15.

State Key Laboratory of Materials Processing and Die & Mould Technology, School of Materials Science and Engineering, Huazhong University of Science and Technology, Wuhan, Hubei, 430074, PR China. Electronic address:

Electrochemical (bio) sensors are now widely acknowledged as a sensitive detection tool for disease diagnosis as well as the detection of numerous species of pharmaceutical, clinical, industrial, food, and environmental origin. The term 'green' demonstrates the development of electrochemical (bio) sensing platforms utilizing biodegradable and sustainable materials. Development of green sensing platforms is one of the most active areas of research minimizing the use of toxic/hazardous reagents and solvent systems, thereby further reducing the production of chemical wastes in sensor fabrication. Read More

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http://dx.doi.org/10.1016/j.bios.2020.112270DOI Listing
September 2020

Global regulator engineering enhances bioelectricity generation in Pseudomonas aeruginosa-inoculated MFCs.

Biosens Bioelectron 2020 Sep 18;163:112269. Epub 2020 May 18.

Key Laboratory of Pollution Process and Environmental Criteria, Ministry of Education, Tianjin Key Laboratory of Urban Ecology Environmental Remediation and Pollution Control, College of Environmental Science and Engineering, Nankai University, Tianjin, 300350, PR China. Electronic address:

The electricigens with high-electroactivity is essential for resolving the low electricity power output (EPT) of microbial fuel cells (MFCs). However, the manipulation by single functional genes shows limitation because electroactivity is a complex phenotype controlled by multiple genes. Herein, global regulator engineering (GRE) was developed to optimize the electroactivity of an isolated strain (Pseudomonas aeruginosa P3-A-11) using an exogenous global regulator IrrE (ionizing radiation resistance E linkage group) as an object. Read More

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http://dx.doi.org/10.1016/j.bios.2020.112269DOI Listing
September 2020

Oxidases, carbon nanotubes, and direct electron transfer: A cautionary tale.

Biosens Bioelectron 2020 Sep 15;163:112260. Epub 2020 May 15.

Department of Chemistry, University of Texas at San Antonio, San Antonio, TX, 78249, USA. Electronic address:

The case study of four FAD-dependent oxidase enzymes is presented in the context of the often-claimed direct electron transfer (DET) to glucose oxidase at carbon nanotubes (CNT). The selected enzymes included d-amino acid (AAOx), alcohol (AOx), pyranose (PyOx), and choline oxidase (ChOx). Each enzyme (E) was mixed with chitosan and CNT (either multi- or single-walled) to form a CNT/E film on the surface of glassy carbon electrode. Read More

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http://dx.doi.org/10.1016/j.bios.2020.112260DOI Listing
September 2020

Modulating the mixed potential for developing biosensors: Direct potentiometric determination of glucose in whole, undiluted blood.

Biosens Bioelectron 2020 Sep 18;163:112302. Epub 2020 May 18.

Department of Analytical and Organic Chemistry, Universitat Rovira I Virgili, 43007, Tarragona, Spain. Electronic address:

The growing demand for tools to generate chemical information in decentralized settings is creating a vast range of opportunities for potentiometric sensors, since their combination of robustness, simplicity of operation and cost can hardly be rivalled by any other technique. In previous works, we have shown that the mixed potential of a Pt electrode can be controlled with analytical purposes using a coating of Nafion, thus providing a way to develop a potentiometric biosensor for glucose. Unfortunately, the linear range of this device did not match the relevant clinical range for glucose in blood. Read More

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http://dx.doi.org/10.1016/j.bios.2020.112302DOI Listing
September 2020

Conformable surface acoustic wave biosensor for E-coli fabricated on PEN plastic film.

Biosens Bioelectron 2020 Sep 2;163:112164. Epub 2020 Apr 2.

Center for Biomolecular Nanotechnologies, Istituto Italiano di Tecnologia, Via Barsanti snc, Arnesano, Italy; University of Salento, Department of Innovation Engineering, Campus Ecotekne, Via Monteroni, Lecce, Italy.

Over the last decades, great effort has gone into developing new biosensor technologies for applications in different fields such as disease diagnosis and detection of pollutants in water and food. Global developments in robotic, IoT technologies and in healthcare sensors require new flexible sensor technologies that are low cost and built from sustainable and reusable or recyclable materials. One of the most promising technologies is based on the development of surface acoustic wave (SAW) flexible biosensors, which are highly reproducible, reliable and wirelessly controllable. Read More

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http://dx.doi.org/10.1016/j.bios.2020.112164DOI Listing
September 2020

Machine learning-based design of meta-plasmonic biosensors with negative index metamaterials.

Biosens Bioelectron 2020 Sep 30;164:112335. Epub 2020 May 30.

School of Electrical and Electronic Engineering, Yonsei University, Seoul, 03722, Republic of Korea. Electronic address:

In this work, we explore the performance of plasmonic biosensor designs that integrate metamaterials based on machine learning algorithms. The meta-plasmonic biosensors were designed for optimized detection of DNA with a layer of double negative metamaterial modeled by an effective medium. An iterative transfer matrix approach was employed to generate training and test sets of resonance characteristics in the parameter space for machine learning. Read More

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http://dx.doi.org/10.1016/j.bios.2020.112335DOI Listing
September 2020

Metal-organic frameworks-based sensitive electrochemiluminescence biosensing.

Biosens Bioelectron 2020 Sep 29;164:112332. Epub 2020 May 29.

State Key Laboratory of Agricultural Microbiology, College of Food Science and Technology, College of Science, Huazhong Agricultural University, Wuhan, 430070, China. Electronic address:

Metal-organic frameworks (MOFs) as porous materials have attracted much attention in various fields such as gas storage, catalysis, separation, and nanomedical engineering. However, their applications in electrochemiluminescence (ECL) biosensing are limited due to the poor conductivity, lack of modification sites, low stability and specificity, and weak biocompatibility. Integrating the functional materials into MOF structures endows MOF composites with improved conductivity and stability and facilitates the design of ECL sensors with multifunctional MOFs, which are potentially advantageous over their individual components. Read More

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http://dx.doi.org/10.1016/j.bios.2020.112332DOI Listing
September 2020

A novel turn-off fluorescent aptasensor for ampicillin detection based on perylenetetracarboxylic acid diimide and gold nanoparticles.

Biosens Bioelectron 2020 Sep 29;164:112329. Epub 2020 May 29.

Department of Chemistry, Faculty of Sciences, Ferdowsi University of Mashhad, Mashhad, Iran. Electronic address:

Herein, a novel turn-off fluorescent aptasensor was developed for selective detection of ampicillin (AMP) at picomolar level based on 3,4,9,10-perylenetetracarboxylic acid diimide (PTCDI) as an affordable and low-cost fluorophore. This aptasensor was designed using aptamer, its complementary strand (CS) and gold nanoparticles (AuNPs). The principle of the sensing method is a decrease in the fluorescence intensity of PTCDI in the presence of free CS. Read More

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http://dx.doi.org/10.1016/j.bios.2020.112329DOI Listing
September 2020

Au/CeO/g-CN heterostructures: Designing a self-powered aptasensor for ultrasensitive detection of Microcystin-LR by density functional theory.

Biosens Bioelectron 2020 Sep 28;164:112328. Epub 2020 May 28.

College of Environmental Science and Engineering, Hunan University, Changsha, 410082, China; Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha, 410082, Hunan, China.

Quantum-sized cerium dioxide (CeO) show high catalytic capability as well as strong light absorption ability owing to its redox couple Ce/Ce and abundant oxygen vacancies, which making it a potential material for designing superior photoelectrochemical (PEC) sensors. However, it has scarcely been applied in the field of PEC sensing, because its wide band gap and aggregation effect can restrict the photoelectric conversion efficiency. Herein, we address these two obstacles by coupling CeO quantum dots (QDs) with graphitic carbon nitride (g-CN) and Au nanoparticles (NPs). Read More

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http://dx.doi.org/10.1016/j.bios.2020.112328DOI Listing
September 2020

SERS biosensors for ultrasensitive detection of multiple biomarkers expressed in cancer cells.

Biosens Bioelectron 2020 Sep 23;164:112326. Epub 2020 May 23.

Department of Chemistry, Chung-Ang University, Seoul, 06974, South Korea. Electronic address:

The design and fabrication of multifunctional surface-enhanced Raman scattering (SERS) nanotags are key issues in their application to biological imaging of cells and tissues. In this study, highly sensitive, reproducible and long-term stable SERS nanotags were developed for the identification of localized distribution of multiple protein biomarkers expressed on breast cancer cells. To enhance the surface electromagnetic fields of Raman reporter molecules, Ag-encapsulated Au (Ag-Au) hollow nanospheres were synthesized. Read More

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http://dx.doi.org/10.1016/j.bios.2020.112326DOI Listing
September 2020

Carboxyl functionalized gold nanorods for sensitive visual detection of biomolecules.

Biosens Bioelectron 2020 Sep 30;164:112324. Epub 2020 May 30.

Senova Gesellschaft für Biowissenschaft und Technik mbH, Industriestraße 8, 99427, Weimar, Germany.

Antibody-modified gold nanomaterials are central to many novel biosensing technologies for example the lateral flow assays technology. The combination of the specificity, provided by antibody-antigen interactions, and the unique optical properties of nanomaterials provide excellent properties for biosensors. Here, we present the use of gold nanorods (GNR) with the localized Surface Plasmon Resonance (LSPR) peak in the visible range for biomarker detection. Read More

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http://dx.doi.org/10.1016/j.bios.2020.112324DOI Listing
September 2020

Ultra-sensitive and high-throughput CRISPR-p owered COVID-19 diagnosis.

Biosens Bioelectron 2020 Sep 23;164:112316. Epub 2020 May 23.

Center for Cellular and Molecular Diagnostics, Tulane University School of Medicine, 1430 Tulane Ave., New Orleans, LA, 70112, USA; Department of Biochemistry and Molecular Biology, Tulane University School of Medicine, 1430 Tulane Ave., New Orleans, LA, 70112, USA. Electronic address:

Recent research suggests that SARS-CoV-2-infected individuals can be highly infectious while asymptomatic or pre-symptomatic, and that an infected person may infect 5.6 other individuals on average. This situation highlights the need for rapid, sensitive SARS-CoV-2 diagnostic assays capable of high-throughput operation that can preferably utilize existing equipment to facilitate broad, large-scale screening efforts. Read More

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http://dx.doi.org/10.1016/j.bios.2020.112316DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7245202PMC
September 2020

An EEG system to detect brain signals from multiple adult zebrafish.

Biosens Bioelectron 2020 Sep 23;164:112315. Epub 2020 May 23.

Department of Robotics Engineering, Daegu Gyeongbuk Institute of Science and Technology (DGIST), Daegu, Republic of Korea. Electronic address:

Zebrafish has been widely used as an experimental animal in many biological processes to study brain functions, neurological disorders and drug toxicity. Electroencephalogram (EEG), which directly measures brain activities, has been used to diagnose and study neurological disorders. Previous studies have recorded EEG signals from adult zebrafish, but the recordings have been only possible from a single individual. Read More

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http://dx.doi.org/10.1016/j.bios.2020.112315DOI Listing
September 2020

Laser-induced graphene interdigitated electrodes for label-free or nanolabel-enhanced highly sensitive capacitive aptamer-based biosensors.

Biosens Bioelectron 2020 Sep 21;164:112272. Epub 2020 May 21.

Institute of Analytical Chemistry, Chemo-and Biosensors, Faculty of Chemistry and Pharmacy, University of Regensburg, 31, 93053, Regensburg, Germany. Electronic address:

Highly porous laser-induced graphene (LIG) is easily generated in complex electrode configurations such as interdigitated electrodes (IDEs). Here, we demonstrate that their superior capacitive response at low frequencies can be exploited in affinity biosensors using thrombin aptamers as model biorecognition elements. Of specific interest was the effect of electrode surface area on capacitance detection, and the comparison between a label-free format and enhancement strategies afforded by carboxy group bearing polymeric nanoparticles or liposomes. Read More

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http://dx.doi.org/10.1016/j.bios.2020.112272DOI Listing
September 2020

Real-time glucose monitoring system containing enzymatic sensor and enzymatic reference electrodes.

Biosens Bioelectron 2020 Sep 30;164:112338. Epub 2020 May 30.

Faculty of Fundamental Sciences, Vilnius Gediminas Technical University, Saulėtekio al. 11, LT-10223, Vilnius, Lithuania; Institute of Biochemistry, Life Sciences Center, Vilnius University, Saulėtekio al. 7, LT-10257, Vilnius, Lithuania. Electronic address:

Every electrochemical biosensor uses two or three electrode setup, which involves sensing electrode for a specific reaction, metal/salt reference electrode (i.e., Ag/AgCl or Hg/HgCl) for the control of the potential and, is some cases, counter electrode for the compensation of the current. Read More

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http://dx.doi.org/10.1016/j.bios.2020.112338DOI Listing
September 2020

Facile and cost-effective liver cancer diagnosis by water-gated organic field-effect transistors.

Biosens Bioelectron 2020 Sep 6;164:112251. Epub 2020 May 6.

Department of Chemistry, Tianjin Key Laboratory of Molecular Optoelectronic Sciences School of Science, Tianjin University, Tianjin, 300072, China; Beijing National Laboratory for Molecular Science, Key Laboratory of Organic Solids, Institution of Chemistry, Chinese Academy of Sciences, Beijing, 100190, China; Joint School of National University of Singapore and Tianjin University, International Campus of Tianjin University, Binhai New City, Fuzhou, 350207, China. Electronic address:

The rise of mortality rate caused by hepatocellular carcinoma accelerates requirements of biosensors for early liver cancer diagnosis and treatment to improve the clinical prognosis and prolong the survival of patients. However, how to realize label-free, low-cost, easy and fast-detection is the major challenge in the design of biosensors. Water-gated organic field-effect transistors efficiently bridge the gap between semiconductor devices and biological systems, leading to an organic device suitable for health or body signal monitoring. Read More

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http://dx.doi.org/10.1016/j.bios.2020.112251DOI Listing
September 2020

Cell barrier characterization in transwell inserts by electrical impedance spectroscopy.

Biosens Bioelectron 2020 Jun 2;165:112345. Epub 2020 Jun 2.

DWI - Leibniz Institute for Interactive Materials, Forckenbeckstr. 50, 52074 Aachen, Germany; RWTH Aachen University, Aachener Verfahrenstechnik-Chemical Process Engineering, Forckenbeckstrasse 51, 52074, Aachen, Germany. Electronic address:

We describe an impedance-based method for cell barrier integrity testing. A four-electrode electrical impedance spectroscopy (EIS) setup can be realized by simply connecting a commercial chopstick-like electrode (STX-1) to a potentiostat allowing monitoring cell barriers cultivated in transwell inserts. Subsequent electric circuit modeling of the electrical impedance results the capacitive properties of the barrier next to the well-known transepithelial electrical resistance (TEER). Read More

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http://dx.doi.org/10.1016/j.bios.2020.112345DOI Listing

Diagnostic methods and potential portable biosensors for coronavirus disease 2019.

Biosens Bioelectron 2020 Jun 2;165:112349. Epub 2020 Jun 2.

Department of Chemical Engineering, Worcester Polytechnic Institute, 100 Institute Road, Worcester, MA, 01609, United States. Electronic address:

Timely detection and diagnosis are urgently needed to guide epidemiological measures, infection control, antiviral treatment, and vaccine research. In this review, biomarkers/indicators for diagnosis of coronavirus disease 2019 (COVID-19) or detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in the environment are summarized and discussed. It is concluded that the detection methods targeting antibodies are not suitable for screening of early and asymptomatic cases since most patients had an antibody response at about 10 days after onset of symptoms. Read More

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http://dx.doi.org/10.1016/j.bios.2020.112349DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7266610PMC

Homogeneous circle-to-circle amplification for real-time optomagnetic detection of SARS-CoV-2 RdRp coding sequence.

Biosens Bioelectron 2020 Jun 3;165:112356. Epub 2020 Jun 3.

Department of Health Technology, Technical University of Denmark, DTU Health Tech, Building 345C, DK-2800, Kongens Lyngby, Denmark. Electronic address:

Circle-to-circle amplification (C2CA) is a specific and precise cascade nucleic acid amplification method consisting of more than one round of padlock probe ligation and rolling circle amplification (RCA). Although C2CA provides a high amplification efficiency with a negligible increase of false-positive risk, it contains several step-by-step operation processes. We herein demonstrate a homogeneous and isothermal nucleic acid quantification strategy based on C2CA and optomagnetic analysis of magnetic nanoparticle (MNP) assembly. Read More

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http://dx.doi.org/10.1016/j.bios.2020.112356DOI Listing

Selection of a metal ligand modified DNAzyme for detecting Ni.

Biosens Bioelectron 2020 May 18;165:112285. Epub 2020 May 18.

Department of Chemistry, University of Waterloo, Waterloo, Ontario, N2L 3G1, Canada. Electronic address:

Nickel is a highly important metal, and the detection of Ni using biosensors is a long-stand analytical challenge. DNA has been widely used for metal detection, although no DNA-based sensors were reported for Ni. DNAzymes are DNA-based catalysts, and they recruit metal ions for catalysis. Read More

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http://dx.doi.org/10.1016/j.bios.2020.112285DOI Listing

Picomolar glyphosate sensitivity of an optical particle-based sensor utilizing biomimetic interaction principles.

Biosens Bioelectron 2020 May 11;165:112262. Epub 2020 May 11.

Institute of Biochemistry, Leipzig University, Johannisallee 21-23, 04103, Leipzig, Germany. Electronic address:

The continually growing use of glyphosate and its critically discussed health and biodiversity risks ask for fast, low cost, on-site sensing technologies for food and water. To address this problem, we designed a highly sensitive sensor built on the remarkably specific recognition of glyphosate by its physiological target enzyme 5-enolpyruvyl-shikimate-3-phosphate synthase (EPSPs). This principle is implemented in an interferometric sensor by using the recently established soft colloidal probe (SCP) technique. Read More

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http://dx.doi.org/10.1016/j.bios.2020.112262DOI Listing

A soft metal-polyphenol capsule-based ultrasensitive immunoassay for electrochemical detection of Epstein-Barr (EB) virus infection.

Biosens Bioelectron 2020 Sep 21;164:112310. Epub 2020 May 21.

School of Food and Biological Engineering, Hefei University of Technology, Hefei, 230009, PR China. Electronic address:

Herein, we have proposed a soft metal-phenolic capsule (sMPC)-based electrochemical immunoassay for ultrasensitive detection of Epstein-Barr virus capsid antigen IgA (EBVCA-IgA), a biomarker of nasopharyngeal carcinoma. Metal probes with large size contain a number of metal ions, which are very beneficial to signal amplification for anodic stripping voltammetry; however, these probes easily precipitate due to their heavy weight, leading to low recognition efficiency and compromised performance. In this study, we demonstrate sMPCs fabricated by metal-coordination interactions exhibit unique surface behavior compared with their solid counterparts, which significantly enhance recognition efficiency and thus improve sensitivity despite of their micrometer size. Read More

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http://dx.doi.org/10.1016/j.bios.2020.112310DOI Listing
September 2020
6.409 Impact Factor

Electrical energy harvesting from ferritin biscrolled carbon nanotube yarn.

Biosens Bioelectron 2020 Sep 22;164:112318. Epub 2020 May 22.

Center for Self-powered Actuation, Department of Biomedical Engineering, Hanyang University, Seoul, 04763, South Korea. Electronic address:

Various studies about harvesting energy for future energy production have been conducted. In particular, replacing batteries in implantable medical devices with electrical harvesting is a great challenge. Here, we have improved the electrical harvesting performance of twisted carbon nanotube yarn, which was previously reported to be an electrical energy harvester, by biscrolling positively charged ferritin protein in a biofluid environment. Read More

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http://dx.doi.org/10.1016/j.bios.2020.112318DOI Listing
September 2020

Designed antifouling peptides planted in conducting polymers through controlled partial doping for electrochemical detection of biomarkers in human serum.

Biosens Bioelectron 2020 Sep 22;164:112317. Epub 2020 May 22.

Key Laboratory of Optic-electric Sensing and Analytical Chemistry for Life Science, MOE, Shandong Key Laboratory of Biochemical Analysis, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao, 266042, China. Electronic address:

An antifouling electrochemical biosensing platform was constructed based on conducting polymer poly(3,4-ethylenedioxythiophene) (PEDOT) planted with designed peptides. The designed peptides containing doping and antifouling sequences were anchored to an electrode surface, followed by the electrochemical polymerization of PEDOT. The negatively charged doping sequence of the peptide was gradually doped into the PEDOT during the polymerization process, and by controlling the polymerization time, it was able to exactly dope the whole doping sequence into the PEDOT film, leaving the antifouling sequence of the peptide stretched out of the PEDOT surface. Read More

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http://dx.doi.org/10.1016/j.bios.2020.112317DOI Listing
September 2020

A chemiresistive biosensor based on a layered graphene oxide/graphene composite for the sensitive and selective detection of circulating miRNA-21.

Biosens Bioelectron 2020 Sep 27;164:112320. Epub 2020 May 27.

Department of Materials Engineering, Ming Chi University of Technology, New Taipei City, 24301, Taiwan, ROC.

In this study we developed a uniform, large-area, layered graphene composite of graphene oxide/graphene (GO/G) for the detection of circulating miRNA-21, a reliable biomarker for early cancer diagnosis. We prepared this layered composite of GO/G through low-damage plasma treatment of bilayer G. The top layer of G was oxidized (i. Read More

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http://dx.doi.org/10.1016/j.bios.2020.112320DOI Listing
September 2020

Mobile healthcare system based on the combination of a lateral flow pad and smartphone for rapid detection of uric acid in whole blood.

Biosens Bioelectron 2020 Sep 21;164:112309. Epub 2020 May 21.

Institute of Medical Science and Technology, National Sun Yat-sen University, Kaohsiung, 80424, Taiwan. Electronic address:

Excessive production of uric acid (UA) in blood may lead to gout, hyperuricaemia and kidney disorder; thus, a fast, simple and reliable biosensor is needed to routinely determine the UA concentration in blood without pretreatment. The purpose of this study was to develop a mobile healthcare (mHealth) system using a drop of blood, which comprised a lateral flow pad (LFP), mesoporous Prussian blue nanoparticles (MPBs) as artificial nanozymes and auto-calculation software for on-site determination of UA in blood and data management. A standard curve was found to be linear in the range of 1. Read More

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http://dx.doi.org/10.1016/j.bios.2020.112309DOI Listing
September 2020

Paper-based ITP technology: An application to specific cancer-derived exosome detection and analysis.

Biosens Bioelectron 2020 Sep 15;164:112292. Epub 2020 May 15.

Gene and Linda Voiland School of Chemical Engineering and Bioengineering, Washington State University, Pullman, WA, USA; Department of Integrative Physiology and Neuroscience, Washington State University, Pullman, WA, USA. Electronic address:

Exosomes derived from cancer cells/tissues have great potential for early cancer diagnostic use, but their clinical potential has not been fully explored because of a lack of cost-effective multiplex approaches capable of effectively isolating and identifying specific exosome populations and analyzing their content biomarkers. This study was aimed at overcoming the technical barrier by developing a paper-based isotachophoresis (ITP) technology capable of 1) rapid isolation and identification of exosomes from both malignant and healthy cells and 2) multiplex detection of selected exosomal protein biomarkers of the target exosomes. The technology integrates the focusing power of ITP and the multiplex capability of paper-based lateral flow to achieve on-board separation of target exosomes from large extracellular vesicles, followed by electrokinetic enrichment of the targets, leading to an ultrasensitive platform for comprehensive exosome analysis. Read More

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http://dx.doi.org/10.1016/j.bios.2020.112292DOI Listing
September 2020

Recent progress in immunosensors for pesticides.

Biosens Bioelectron 2020 Sep 30;164:112255. Epub 2020 Apr 30.

Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100193, China; State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, 210009, China. Electronic address:

Immunosensors for rapid detection of pesticide residues has attracted considerable interest in the past few years for healthcare and environmental monitoring. And the publications have grown exponentially over the past decades, making it a trending hot-spot. Therefore, this review first examines the current situation regarding pesticide residue in various foods, feeds, traditional Chinese medicines and environmental samples. Read More

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http://dx.doi.org/10.1016/j.bios.2020.112255DOI Listing
September 2020

Active N dopant states of electrodes regulate extracellular electron transfer of Shewanella oneidensis MR-1 for bioelectricity generation: Experimental and theoretical investigations.

Biosens Bioelectron 2020 Jul 23;160:112231. Epub 2020 Apr 23.

CAS Key Laboratory of Urban Pollutant Conversion, Collaborative Innovation Centre of Suzhou Nano Science and Technology, Department of Applied Chemistry, University of Science and Technology of China, Hefei, China. Electronic address:

Anodic N doping is an effective way to improve power generation of bioelectrochemical systems (BESs), but the role of various active N dopant states of the anode on BES performance is still unclear. Herein, the effect of anodic active N dopant states on bioelectricity generation of Shewanella oneidensis MR-1 inoculated BESs particularly including microbial extracellular electron transfer (EET) was explored using experiments and theoretical simulations. It was found a positive linear correlation between the peak current density of BESs and pyrrolic N content of the anode, which would mainly ascribe to the enhancement of both direct electron transfer (DET) and mediated electron transfer (MET) of S. Read More

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http://dx.doi.org/10.1016/j.bios.2020.112231DOI Listing

Fast and sensitive diagnosis of autoimmune disorders through amperometric biosensing of serum anti-dsDNA autoantibodies.

Biosens Bioelectron 2020 Jul 23;160:112233. Epub 2020 Apr 23.

Department of Analytical Chemistry, Faculty of Chemistry, University Complutense of Madrid, Ciudad Universitaria S/N, 28040, Madrid, Spain.

This work reports the first amperometric biosensor involving the use of neutravidin-functionalized magnetic microbeads (NA-MBs) modified with a biotinylated-anti-dsDNA (b-dsDNA) as efficient magnetic microcarriers to selectively capture anti-dsDNA autoantibodies (IgG, IgA and IgM AAbs) present in the sera of patients with rheumatoid arthritis (RA). Subsequently, the attached anti-dsDNA AAbs are detected with a mixture of conventional HRP-labeled secondary antibodies (HRP-anti-human IgG/IgM/IgA mixture). The biorecognition event is monitored by amperometric transduction using the hydroquinone (HQ)/HO system upon capturing the modified MBs on the surface of screen-printed carbon electrodes (SPCEs). Read More

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http://dx.doi.org/10.1016/j.bios.2020.112233DOI Listing

Specific recognition of ion channel blocker by high-content cardiomyocyte electromechanical integrated correlation.

Biosens Bioelectron 2020 Aug 8;162:112273. Epub 2020 May 8.

The First Affiliated Hospital of Sun Yat-sen University, State Key Laboratory of Optoelectronic Materials and Technologies, Guangdong Province Key Laboratory of Display Material and Technology, School of Electronics and Information Technology, Sun Yat-sen University, Guangzhou, 510006, China. Electronic address:

Cardiac arrhythmia and drug-induced cardiotoxicity seriously threaten the human life. To develop antiarrhythmic agents and prevent the drug-induced cardiotoxicity, it is demanded to explore the high-specificity and high-efficiency drug screening platforms for preclinical investigations. Here, a specific electromechanical integrated correlation (EMIC) model was established based on the synchronized signal recording of cardiomyocyte-based biosensing system. Read More

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http://dx.doi.org/10.1016/j.bios.2020.112273DOI Listing
August 2020
6.409 Impact Factor

A 96-well high-throughput, rapid-screening platform of extracellular electron transfer in microbial fuel cells.

Biosens Bioelectron 2020 Aug 8;162:112259. Epub 2020 May 8.

Bioelectronics & Microsystems Laboratory, Department of Electrical & Computer Engineering, State University of New York-Binghamton, Binghamton, NY, 13902-6000, USA. Electronic address:

Microbial extracellular electron transfer (EET) stimulates a plethora of intellectual concepts leading to potential applications that offer environmentally sustainable advances in the fields of biofuels, wastewater treatment, bioremediation, desalination, and biosensing. Despite its vast potential and remarkable research efforts to date, bacterial electrogenicity is arguably the most underdeveloped technology used to confront the aforementioned challenges. Severe limitations are placed in the intrinsic energy and electron transfer processes of naturally occurring microorganisms. Read More

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http://dx.doi.org/10.1016/j.bios.2020.112259DOI Listing

Multienzyme chemiluminescent foldable biosensor for on-site detection of acetylcholinesterase inhibitors.

Biosens Bioelectron 2020 Aug 29;162:112232. Epub 2020 Apr 29.

Department of Chemistry "Giacomo Ciamician", University of Bologna, Via Selmi 2, 40126, Bologna, Italy; INBB, Istituto Nazionale di Biostrutture e Biosistemi, 00136, Rome, Italy; Health Sciences and Technologies-Interdepartmental Center for Industrial Research (HST-ICIR), University of Bologna, via Tolara di Sopra 41/E, 40064, Ozzano dell'Emilia, Bologna, Italy. Electronic address:

The rapid hydrolysis of acetylcholine (ACh), one of the key neurotransmitters in the human body, by the enzyme acetylcholinesterase (AChE) is fundamental for the termination of ACh impulse transmission. Several chemicals, including organophosphorus (OP) pesticides, warfare agents and drugs, are AChE reversible or irreversible inhibitors, thus their rapid on-site detection is of primary importance. Here we report for the first time a chemiluminescence (CL) foldable paper-based biosensor for detection of AChE inhibitors. Read More

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http://dx.doi.org/10.1016/j.bios.2020.112232DOI Listing

Flexible and highly ordered nanopillar electrochemical sensor for sensitive insulin evaluation.

Biosens Bioelectron 2020 Aug 1;161:112252. Epub 2020 May 1.

Nano-bio Application Team, National NanoFab Center (NNFC), Daejeon, 34141, Republic of Korea. Electronic address:

In line with growing interest in obesity management, there has been an increase in the amount of research focused on highly sensitive analysis systems for a small number of biomarers. In this paper, we introduce the highly ordered nanopillar electrode, featuring a high aspect ratio surface area that enables enhanced electron transfer. For fabrication of the flexible electrode, gold was evaporated by electronic beam lithography on polyurethane (PU), which has high flexibility. Read More

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http://dx.doi.org/10.1016/j.bios.2020.112252DOI Listing

Electrically guided interventional radiology, in-vivo electrochemical tracing of suspicious lesions to breast cancer prior to core needle biopsy.

Biosens Bioelectron 2020 Aug 16;161:112209. Epub 2020 Apr 16.

Nano Bio Electronic Devices Lab, School of Electrical and Computer Engineering, College of Engineering, University of Tehran, P.O. Box 14395/515, Tehran, Iran; Cancer Electronics Research Group, School of Electrical and Computer Engineering, College of Engineering, University of Tehran, P.O. Box 14395/515, Tehran, Iran. Electronic address:

An electrochemical biopsy probe was designed and fabricated to detect cancer tumors under the sonography guide without the need for any sample dissection (biopsy). The system was based on recording the hypoxic function of cancer tumors by Multi-wall carbon nanotubes (MWCNTs) sensing agents had been decorated on the tip of the needle electrodes by an electrostatic deposition method. This system named BGP successfully distinct 4T1 and MC4L2 breast tumors from normal lesions. Read More

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http://dx.doi.org/10.1016/j.bios.2020.112209DOI Listing

A dual-mode PCT electrochemical immunosensor with CuCoS bimetallic sulfides as enhancer.

Biosens Bioelectron 2020 Sep 12;163:112280. Epub 2020 May 12.

Collaborative Innovation Center for Green Chemical Manufacturing and Accurate Detection, Key Laboratory of Interfacial Reaction & Sensing Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan, 250022, PR China.

A method for the detection of procalcitonin (PCT) with improved accuracy is urgently needed. A dual-mode electrochemical immunosensor with CuCoS-Au bimetallic sulfides as enhancer for accurate detection of PCT was fabricated in this work. The immunosensor not only displays a heavy square wave voltammetry (SWV) signal due to the electron transfer of Cu and Cu but not the addition of electron mediators. Read More

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http://dx.doi.org/10.1016/j.bios.2020.112280DOI Listing
September 2020
6.409 Impact Factor

Time-resolved fluorescence resonance energy transfer-based lateral flow immunoassay using a raspberry-type europium particle and a single membrane for the detection of cardiac troponin I.

Biosens Bioelectron 2020 Sep 11;163:112284. Epub 2020 May 11.

Department of Molecular Science and Technology, Ajou University, Suwon 16499, South Korea. Electronic address:

Herein, we report a novel lateral flow immunoassay (LFIA) system for detecting cardiac troponin I (cTnI) in serum using the time-resolved fluorescence resonance energy transfer (TR-FRET) technique and the fusion 5 membrane. The fusion 5 membrane is used as a strip for LFIA, and it is constructed without additional matrices (such as a sample or conjugation pad). Although this strategy for constructing the LFIA strip is quite simple and cost-effective, LFIA is still not suitable for the analysis of biomarkers that require high sensitivity, such as cTnI. Read More

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http://dx.doi.org/10.1016/j.bios.2020.112284DOI Listing
September 2020

Highly selective and sensitive detection of hydrogen sulfide in aqueous medium and live cells using peptide-based bioprobe to mimic the binding sites of the ceruloplasmin for Cu(II) ions.

Biosens Bioelectron 2020 Sep 11;163:112283. Epub 2020 May 11.

Chemical Synthesis and Pollution Control Key Laboratory of Sichuan Province, College of Chemistry and Chemical Engineering, China West Normal University, Shida Road 1#, Nanchong, 637009, PR China.

Hydrogen sulfide (HS) plays a vital role in biological systems, so it is important to develop new methods for monitoring HS with high selectively and sensitivity. In this study, we report the efficient solid phase synthesis of a new peptide-based bioprobe L that mimics the Cu(II) binding sites of ceruloplasmin. L exhibits highly sensitive colorimetric and fluorescent "turn off" responses to Cu with high selectively among various metal ions over a broad pH range in 100% aqueous solutions. Read More

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http://dx.doi.org/10.1016/j.bios.2020.112283DOI Listing
September 2020

Advanced "lab-on-a-chip" to detect viruses - Current challenges and future perspectives.

Biosens Bioelectron 2020 Sep 12;163:112291. Epub 2020 May 12.

Research Centre for Analytical Instrumentation, Institute of Cyber-Systems and Control, State Key Laboratory of Industrial Control Technology, Zhejiang University, Hangzhou, Zhejiang Province, 310058, China; College of Life Sciences, Zhejiang University, Hangzhou, 310058, Zhejiang, China. Electronic address:

Massive viral outbreaks draw attention to viruses that have not been thoroughly studied or understood. In recent decades, microfluidic chips, known as "lab-on-a-chip", appears as a promising tool for the detection of viruses. Here, we review the development of microfluidic chips that could be used in response to viral detection, specifically for viruses involved in more recent outbreaks. Read More

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http://dx.doi.org/10.1016/j.bios.2020.112291DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7215165PMC
September 2020

Immunoassays on thiol-ene synthetic paper generate a superior fluorescence signal.

Biosens Bioelectron 2020 Sep 11;163:112279. Epub 2020 May 11.

KTH Royal Institute of Technology, Micro and Nanosystems, Malvinas väg 10, 100 44, Stockholm, Sweden. Electronic address:

The fluorescence-based detection of biological complexes on solid substrates is widely used in microarrays and lateral flow tests. Here, we investigate thiol-ene micropillar scaffold sheets ("synthetic paper") as the solid substrate in such assays. Compared to state-of-the-art glass and nitrocellulose substrates, assays on synthetic paper provide a stronger fluorescence signal, similar or better reproducibility, lower limit of detection (LOD), and the possibility of working with lower immunoreagent concentrations. Read More

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http://dx.doi.org/10.1016/j.bios.2020.112279DOI Listing
September 2020

Fast scan voltammetry-derived ultrasensitive Faraday cage-type electrochemical immunoassay for large-size targets.

Biosens Bioelectron 2020 Sep 11;163:112277. Epub 2020 May 11.

School of Marine Sciences, Ningbo University, Ningbo, 315211, PR China. Electronic address:

Electrochemical immunoassay (ECIA) is an important method for rapid, on-site detection of various analytes. However, its detection sensitivity is greatly limited by the traditional sandwich-type sensor construction mode, especially in the case of large-size targets such as pathogenic bacteria with micron size. Herein, we developed a Faraday cage-type sensing mode to build an electrochemical immunosensor based on a functionalized two-dimensional conductive nanomaterial, which could provide a platform to assemble a large number of electrochemical signal labels and a good support for the expansion of electrode surface. Read More

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http://dx.doi.org/10.1016/j.bios.2020.112277DOI Listing
September 2020

The impact of biosensing in a pandemic outbreak: COVID-19.

Biosens Bioelectron 2020 Sep 6;163:112274. Epub 2020 May 6.

Freiburg Center for Interactive Materials and Bioinspired Technologies (FIT), University of Freiburg, Georges-Koehler-Allee 105, Freiburg, 79110, Germany; Department of Microsystems Engineering (IMTEK), Laboratory for Sensors, University of Freiburg, Georges-Koehler-Allee 103, Freiburg, 79110, Germany. Electronic address:

COVID-19 pandemic outbreak is the most astounding scene ever experienced in the XXI century. In this opinionated review, we underscore the crucial role of biosensing to handle with such situations. As a matter of fact, testing accelerates life-saving decisions on treatment and isolation of COVID-19 patients in an early stage, and thereby, decelerating or even preventing the spread of such emerging infectious diseases. Read More

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http://dx.doi.org/10.1016/j.bios.2020.112274DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7202811PMC
September 2020