48,702 results match your criteria Bioscience of microbiota food and health[Journal]


Terrestrial organic matter increases zooplankton methylmercury accumulation in a brown-water boreal lake.

Sci Total Environ 2019 Mar 29;674:9-18. Epub 2019 Mar 29.

Department of Bioscience, University of Oslo, Blindernveien 31, 0371 Oslo, Norway. Electronic address:

Increases in terrestrial organic matter (tOM) transport from catchments to boreal lakes can affect methylmercury (MeHg) accumulation in aquatic biota both directly by increasing concentrations of aqueous MeHg, and indirectly through effects on MeHg bioavailability and on energy pathways in the lower food web. We carried out a detailed seasonal study of water chemistry, zooplankton diet, and MeHg accumulation in zooplankton in two lakes with contrasting tOM concentrations. Between-lake differences explained 51% of the variability in our water chemistry data, with no observed effect of season or sampling depth, contrary to our expectations. Read More

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http://dx.doi.org/10.1016/j.scitotenv.2019.03.446DOI Listing

Outer membrane vesicles engineered to express membrane-bound antigen program dendritic cells for cross-presentation to CD8 T cells.

Acta Biomater 2019 Apr 16. Epub 2019 Apr 16.

Department of Molecular Cell Biology and Immunology, Amsterdam UMC, Location VUmc, Amsterdam, The Netherlands. Electronic address:

Outer membrane vesicles (OMVs) are vesicular nano-particles produced by Gram-negative bacteria that are recently being explored as vaccine vector. The fact that OMVs can be efficiently produced by a hypervesiculating Salmonella typhimurium strain, are packed with naturally-occurring adjuvants like lipopolysaccharides (LPS), and can be engineered to express any antigen of choice, makes them ideal candidates for vaccinology. However, it is unclear whether OMVs induce dendritic cell (DC)-mediated antigen-specific T cell responses and how immune activation is coordinated. Read More

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http://dx.doi.org/10.1016/j.actbio.2019.04.033DOI Listing

Single-cell correlations of mRNA and protein content in a human monocytic cell line after LPS stimulation.

PLoS One 2019 19;14(4):e0215602. Epub 2019 Apr 19.

Center for Integrated Nanotechnologies, Los Alamos National Laboratory, Los Alamos, New Mexico, United States of America.

The heterogeneity of mRNA and protein expression at the single-cell level can reveal fundamental information about cellular response to external stimuli, including the sensitivity, timing, and regulatory interactions of genes. Here we describe a fully automated system to digitally count the intron, mRNA, and protein content of up to five genes of interest simultaneously in single-cells. Full system automation of 3D microscope scans and custom image analysis routines allows hundreds of individual cells to be automatically segmented and the mRNA-protein content to be digitally counted. Read More

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0215602PLOS

In vitro chondroprotective potential of Senna alata and Senna tora in porcine cartilage explants and their species differentiation by DNA barcoding-high resolution melting (Bar-HRM) analysis.

PLoS One 2019 19;14(4):e0215664. Epub 2019 Apr 19.

Excellent Center in Veterinary Bioscience, Chiang Mai University, Chiang Mai, Thailand.

Senna species and anthraquinone derivatives generated by these organisms, rhein and aloe-emodin, exert anti-inflammatory effects. These species present a similar morphology but produce different ingredients when they are used as medicinal products. In this study, a DNA barcoding- (Bar-) high-resolution melting (HRM) technique was developed using internal transcribed sequence 2 (ITS2) to differentiate between Senna alata and Senna tora as a result of significant differences in their melting profiles. Read More

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0215664PLOS

LIDAR explains diversity of plants, fungi, lichens and bryophytes across multiple habitats and large geographic extent.

Ecol Appl 2019 Apr 19:e01907. Epub 2019 Apr 19.

Section for Ecoinformatics and Biodiversity, Department of Bioscience, Aarhus University, Ny Munkegade 114, DK, 8000, Aarhus C, Denmark.

Effective planning and nature management require spatially accurate and comprehensive measures of the factors important for biodiversity. LIDAR (Light detection and ranging) can provide exactly this, and is therefore a promising technology to support future nature management and related applications. However, until now studies evaluating the potential of LIDAR for this field have been highly limited in scope. Read More

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http://dx.doi.org/10.1002/eap.1907DOI Listing

Differential expression of tescalcin by modification of promoter methylation controls cell survival in gastric cancer cells.

Oncol Rep 2019 Apr 4. Epub 2019 Apr 4.

Immunotherapy Convergence Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 34824, Republic of Korea.

The EF‑hand calcium binding protein tescalcin (TESC) is highly expressed in various human and mouse cancer tissues and is therefore considered a potential oncogene. However, the underlying mechanism that governs TESC expression remains unclear. Emerging evidence suggests that TESC expression is under epigenetic regulation. Read More

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http://dx.doi.org/10.3892/or.2019.7099DOI Listing

Synthesis of Alkynyl Sulfides by Copper-Catalyzed Thiolation of Terminal Alkynes Using Thiosulfonates.

Org Lett 2019 Apr 19. Epub 2019 Apr 19.

Laboratory of Chemical Bioscience, Institute of Biomaterials and Bioengineering , Tokyo Medical and Dental University (TMDU) , 2-3-10 Kanda-Surugadai , Chiyoda-ku, Tokyo 101-0062 , Japan.

A mild and odorless copper-catalyzed thiolation of terminal alkynes with thiosulfonates is described. The broad substrate scope provides convenient access to a wide variety of sulfur-containing heterocycles. In particular, divergent benzoheteroles are efficiently prepared in a simple manner by thiolation of ethynylbenzenes bearing ortho-nucleophilic functional groups followed by iodocyclization. Read More

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http://pubs.acs.org/doi/10.1021/acs.orglett.9b00875
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http://dx.doi.org/10.1021/acs.orglett.9b00875DOI Listing
April 2019
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Altered donor P2X7 activity in human leukocytes correlates with P2RX7 genotype but does not affect the development of graft-versus-host disease in humanised mice.

Purinergic Signal 2019 Apr 18. Epub 2019 Apr 18.

School of Chemistry and Molecular Bioscience, University of Wollongong, Wollongong, NSW, 2522, Australia.

Graft-versus-host disease (GVHD) is a life-threatening consequence of allogeneic haematopoietic stem cell transplantation, a curative therapy for haematological malignancies. The ATP-gated P2X7 receptor channel is implicated in the development of GVHD. P2X7 activity on human leukocytes can be influenced by gain-of-function (GOF) and loss-of-function (LOF) single nucleotide polymorphisms (SNPs) in the P2RX7 gene. Read More

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http://dx.doi.org/10.1007/s11302-019-09651-8DOI Listing

Extracellular Vesicles in the Synovial Joint: Is there a Role in the Pathophysiology of Osteoarthritis?

Malays Orthop J 2019 Mar;13(1):1-7

Swansea University Medical School, Swansea University, Swansea, United Kingdom.

The role of extracellular vesicles (EV) in osteoarthritis has become the focus of much research. These vesicles were isolated from several cell types found in synovial joint including chondrocytes and synovium. As articular cartilage is an avascular tissue surrounded by synovial fluid, it is believed that EV might play a crucial role in the homeostasis of cartilage and also could hold key information in the pathogenesis of osteoarthritis. Read More

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http://www.morthoj.org/2019/v13n1/extracellular-vesicles.pdf
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http://dx.doi.org/10.5704/MOJ.1903.012DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6459045PMC
March 2019
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Methylation at the C-3' in D-Ring of Strigolactone Analogs Reduces Biological Activity in Root Parasitic Plants and Rice.

Front Plant Sci 2019 2;10:353. Epub 2019 Apr 2.

The BioActives Lab, Division of Biological and Environmental Sciences and Engineering, King Abdullah University of Science and Technology, Thuwal, Saudi Arabia.

Strigolactones (SLs) regulate plant development and induce seed germination in obligate root parasitic weeds, e.g. spp. Read More

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http://dx.doi.org/10.3389/fpls.2019.00353DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6455008PMC

Egg Protein Transferrin-Derived Peptides IRW and IQW Regulate -Induced, Inflammation-Related Microbial and Metabolomic Profiles.

Front Microbiol 2019 3;10:643. Epub 2019 Apr 3.

College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, China.

Bioactive peptides that target the gastrointestinal tract can strongly affect the health of animals and humans. This study aimed to evaluate the abilities of two peptides derived from egg albumin transferrin, IRW and IQW, to treat enteritis in a mouse model of -induced colitis by evaluating serum metabolomics and gut microbes. Forty-eight mice were randomly assigned to six groups: basal diet (CTRL), intragastric administration (CR), basal diet with 0. Read More

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http://dx.doi.org/10.3389/fmicb.2019.00643DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6456682PMC

Prevalence and Characterization of Isolated From Pasteurized Milk in China.

Front Microbiol 2019 2;10:641. Epub 2019 Apr 2.

Guangdong Institute of Microbiology, State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, Guangzhou, China.

is one of the most important food-borne pathogens globally. It produces various toxins and invasive enzymes and can be found in numerous food products. Milk is an important source of staphylococcal food poisoning. Read More

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https://www.frontiersin.org/article/10.3389/fmicb.2019.00641
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http://dx.doi.org/10.3389/fmicb.2019.00641DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6454862PMC
April 2019
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Corrigendum: MazF Specifically Recognises the UGG Motif and Promotes Selective RNA Degradation.

Front Microbiol 2019 3;10:634. Epub 2019 Apr 3.

Department of Life Science and Medical Bioscience, Waseda University, Tokyo, Japan.

[This corrects the article DOI: 10.3389/fmicb.2018. Read More

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https://www.frontiersin.org/article/10.3389/fmicb.2019.00634
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http://dx.doi.org/10.3389/fmicb.2019.00634DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6456890PMC
April 2019
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4--Carboxymethylascochlorin Inhibits Expression Levels of on Inflammation-Related Cytokines and Matrix Metalloproteinase-9 Through NF-κB/MAPK/TLR4 Signaling Pathway in LPS-Activated RAW264.7 Cells.

Front Pharmacol 2019 27;10:304. Epub 2019 Mar 27.

Molecular and Cellular Glycobiology Unit, Department of Biological Sciences, Sungkyunkwan University, Suwon, South Korea.

Toll-like receptor 4 (TLR4) and matrix metalloproteinase-9 (MMP-9) are known to play important roles in inflammatory diseases such as arteriosclerosis and plaque instability. The purpose of this study was to perform the effect of 4--carboxymethylascochlorin (AS-6) on MMP-9 expression in lipopolysaccharide (LPS)-induced murine macrophages and signaling pathway involved in its anti-inflammatory effect. Effect of AS-6 on MAPK/NF-κB/TLR4 signaling pathway in LPS-activated murine macrophages was examined using ELISA, Western blotting, reverse transcription polymerase chain reaction (RT-PCR) and fluorescence immunoassay. Read More

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https://www.frontiersin.org/article/10.3389/fphar.2019.00304
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http://dx.doi.org/10.3389/fphar.2019.00304DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6445864PMC
March 2019
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Assessing causality in the association between attention-deficit/hyperactivity disorder and obesity: a Mendelian randomization study.

Int J Obes (Lond) 2019 Apr 18. Epub 2019 Apr 18.

Postgraduate Program in Epidemiology, Universidade Federal de Pelotas, Pelotas, Brazil.

Background/objectives: Attention-deficit hyperactivity disorder (ADHD), one of the most common neurodevelopmental disorders in childhood and adolescence, is associated with obesity in observational studies. However, it is unclear whether ADHD contributes to, results from or is merely correlated with obesity. This study evaluates the presence and direction of a causal effect between ADHD and obesity. Read More

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http://dx.doi.org/10.1038/s41366-019-0346-8DOI Listing

Hippo signaling is intrinsically regulated during cell cycle progression by APC/C.

Proc Natl Acad Sci U S A 2019 Apr 18. Epub 2019 Apr 18.

Department of Developmental Biology, Harvard Stem Cell Institute, Harvard School of Dental Medicine, Boston, MA 02215;

The Hippo-YAP/TAZ signaling pathway plays a pivotal role in growth control during development and regeneration and its dysregulation is widely implicated in various cancers. To further understand the cellular and molecular mechanisms underlying Hippo signaling regulation, we have found that activities of core Hippo signaling components, large tumor suppressor (LATS) kinases and YAP/TAZ transcription factors, oscillate during mitotic cell cycle. We further identified that the anaphase-promoting complex/cyclosome (APC/C) E3 ubiquitin ligase complex, which plays a key role governing eukaryotic cell cycle progression, intrinsically regulates Hippo signaling activities. Read More

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http://dx.doi.org/10.1073/pnas.1821370116DOI Listing
April 2019
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Down-regulation of human DAB2IP Gene Expression in Renal Cell Carcinoma Results in Resistance to Ionizing Radiation.

Clin Cancer Res 2019 Apr 18. Epub 2019 Apr 18.

Urology, UT Southwestern Medical Center

Purpose: Renal cell carcinoma (RCC) is known to be highly radio-resistant but the mechanisms associated with radio-resistance have remained elusive. We found DAB2IP frequently down-regulated in RCC, is associated with radio-resistance. In this study, we investigated the underlying mechanism regulating radio-resistance by DAB2IP and developed appropriate treatment. Read More

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http://dx.doi.org/10.1158/1078-0432.CCR-18-3004DOI Listing

Genome Sequence of a Staphylococcus xylosus Clinical Isolate, Strain SMA0341-04 (UGA5), from Siaya County Referral Hospital in Siaya, Kenya.

Microbiol Resour Announc 2019 Apr 18;8(16). Epub 2019 Apr 18.

Physical Chemistry and Applied Spectroscopy, Chemistry Division, Los Alamos National Laboratory, Los Alamos, New Mexico, USA

We report here the genome sequence of a clinical isolate, strain SMA0341-04 (UGA5), which contains one chromosome and at least one plasmid. Notably, strain SMA0341-04 (UGA5) contains the tetracycline efflux major facilitator superfamily (MFS) transporter () gene. Read More

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http://genomea.asm.org/lookup/doi/10.1128/MRA.01625-18
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http://dx.doi.org/10.1128/MRA.01625-18DOI Listing
April 2019
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Simultaneous immunofluorescent imaging of gliadins, low molecular weight glutenins, and high molecular weight glutenins in wheat flour dough with antibody-quantum dot complexes.

Food Res Int 2019 Jun 18;120:776-783. Epub 2018 Nov 18.

Department of Food Science, College of Agriculture, Purdue University, West Lafayette, IN 47907, United States. Electronic address:

Gluten proteins and their impact in the quality of wheat based food products is well known. In order visualize the 'in situ' distribution of low molecular weight glutenins, high molecular weight glutenins, and gliadins simultaneously in wheat doughs we needed to overcome and eliminate dough auto-fluorescence, and to develop a reliable immunostaining procedure for their simultaneous detection in wheat doughs. We are studying different auto-fluorescence quenchers used in biological fluorescent imaging and their effect on dough auto-fluorescence removal, and the effect of different fixative mediums on the adhesion of wheat flours doughs onto microscope slides. Read More

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http://dx.doi.org/10.1016/j.foodres.2018.11.038DOI Listing

Fusion of the N-terminal domain of Pseudomonas sp. phytase with Bacillus sp. phytase and its effects on optimal temperature and catalytic efficiency.

Enzyme Microb Technol 2019 Jul 3;126:69-76. Epub 2019 Apr 3.

Department of Biotechnology, Pukyong National University, Busan, 48513, Republic of Korea. Electronic address:

The beta-propeller phytase (BPP) is an enzyme that hydrolyzes phyate to release inorganic phosphorus. The BPP produced by Pseudomonas sp. FB15 (PSphy) possesses an additional N-terminal domain that is not present in BPP produced by other Bacillus species. Read More

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http://dx.doi.org/10.1016/j.enzmictec.2019.04.002DOI Listing

Differentiating the geographical origin of Ethiopian coffee using XRF- and ICP-based multi-element and stable isotope profiling.

Food Chem 2019 Aug 27;290:295-307. Epub 2019 Mar 27.

Isotope Bioscience Laboratory - ISOFYS, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, 9000 Gent, Belgium.

To test the potential of different analytical tools to determine the geographical origin of Ethiopian coffee, 103 green arabica coffee samples from four coffee regions in Ethiopia were subjected to multi-elements and δC, δN and δO determinations. Multi-elements were determined by using inductively coupled plasma (ICP)- and X-ray fluorescence spectrometry (XRF)-based techniques, and δC, δN and δO were determined by using elemental analyzer-isotope ratio mass spectrometry. Using linear discriminant analysis, XRF-based multi-elements with and without δC appeared to be most effective in discriminating the geographical origin of coffee, giving higher classification accuracy (89 and 86%, respectively) than ICP-based multi-elements with and without stable isotopes (80%, each). Read More

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https://linkinghub.elsevier.com/retrieve/pii/S03088146193062
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http://dx.doi.org/10.1016/j.foodchem.2019.03.135DOI Listing
August 2019
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Sexually dimorphic germ cell identity in mammals.

Curr Top Dev Biol 2019 20;134:253-288. Epub 2019 Feb 20.

School of Biomedical Sciences, The University of Queensland, Brisbane, QLD, Australia; Institute for Molecular Bioscience, The University of Queensland, Brisbane, QLD, Australia. Electronic address:

Germ cells are the stem cells of the species. Thus, it is critical that we have a good understanding of how they are specified, how the somatic cells instruct and support them, how they commit to one or other sex, and how they ultimately develop into functional gametes. Here, we focus on specifics of how sexual fate is determined during fetal life. Read More

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http://dx.doi.org/10.1016/bs.ctdb.2019.01.011DOI Listing
February 2019

Bazam: a rapid method for read extraction and realignment of high-throughput sequencing data.

Genome Biol 2019 Apr 18;20(1):78. Epub 2019 Apr 18.

Bioinformatics, Murdoch Children's Research Institute, Royal Children's Hospital, Flemington Road, Parkville, Victoria, 3052, Australia.

The vast quantities of short-read sequencing data being generated are often exchanged and stored as aligned reads. However, aligned data becomes outdated as new reference genomes and alignment methods become available. Here we describe Bazam, a tool that efficiently extracts the original paired FASTQ from alignment files (BAM or CRAM format) in a format that directly allows efficient realignment. Read More

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http://dx.doi.org/10.1186/s13059-019-1688-1DOI Listing

Anti-photoaging effect of skin cream manufactured with ziyuglycoside I isolated from Sanguisorba officinalis on ultraviolet B-induced hairless mice.

Biosci Biotechnol Biochem 2019 Apr 19:1-8. Epub 2019 Apr 19.

a Department of Beauty Science , Kwangju Women's University , Gwangju , South Korea.

In this study, skin cream containing ziyuglycoside I isolated from Sanguisorba officinalis was manufactured and examined the protective effects of the skin cream against UVB-induced hairless mice. UVB-induced hairless mice were topically treated with the skin cream once a day for 5 weeks. Application of the skin cream did not exhibit side effect on body growth showing normal body weight and food efficiency in the mice. Read More

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http://dx.doi.org/10.1080/09168451.2019.1607247DOI Listing

Identification of the minimum region of flatfish myostatin propeptide (Pep45-65) for myostatin inhibition and its potential to enhance muscle growth and performance in animals.

PLoS One 2019 18;14(4):e0215298. Epub 2019 Apr 18.

Department of Marine Molecular Bioscience, Gangneung-Wonju National University, Gangneung-si, Ganwon-do, Korea.

Myostatin (MSTN) negatively regulates skeletal muscle growth, and its activity is inhibited by the binding of MSTN propeptide (MSTNpro), the N-terminal domain of proMSTN that is proteolytically cleaved from the proMSTN. Partial sequences from the N-terminal side of MSTNpro have shown to be sufficient to inhibit MSTN activity. In this study, to determine the minimum size of flatfish MSTNpro for MSTN inhibition, various truncated forms of flatfish MSTNpro with N-terminal maltose binding protein (MBP) fusion were expressed in E. Read More

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0215298PLOS

Capability of iturin from Bacillus subtilis to inhibit Candida albicans in vitro and in vivo.

Appl Microbiol Biotechnol 2019 Apr 17. Epub 2019 Apr 17.

Key Laboratory for Space Bioscience and Space Biotechnology, School of Life Sciences, Northwestern Polytechnical University, 127 Youyi West Road, Xi'an, 710072, Shaanxi Province, China.

Candida albicans is a fungal pathogen that is difficult to cure clinically. The current clinic C. albicans-inhibiting drugs are very harmful to humans. Read More

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http://link.springer.com/10.1007/s00253-019-09805-z
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http://dx.doi.org/10.1007/s00253-019-09805-zDOI Listing
April 2019
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Assessing oil accumulation in the oleaginous yeast JRC1 using dairy waste cheese whey as a substrate.

3 Biotech 2019 May 10;9(5):173. Epub 2019 Apr 10.

Environmental Biotechnology Laboratory, Department of Bioscience and Bioengineering, Indian Institute of Technology Jodhpur (IIT J), Jodhpur, Rajasthan 342037 India.

This study assesses the potential for the lipid production by the oleaginous yeast JRC1 using dairy industry waste cheese whey as a substrate. Cheese whey was used either untreated (UCW) or deproteinized (DCW) at different concentrations (25-100%) to serve as the carbon and energy source. Both UCW and DCW supported high biomass and lipid productivities. Read More

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http://dx.doi.org/10.1007/s13205-019-1701-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6458212PMC

The message from the matrix-should we listen more closely?

J Thorac Dis 2019 Mar;11(Suppl 3):S230-S233

Nordic Bioscience A/S, Herlev Hovedgade, Herlev, Denmark.

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http://jtd.amegroups.com/article/view/27255/20198
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http://dx.doi.org/10.21037/jtd.2019.01.68DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6424747PMC
March 2019
1 Read

Re-treatment with abatacept plus methotrexate for disease flare after complete treatment withdrawal in patients with early rheumatoid arthritis: 2-year results from the AVERT study.

RMD Open 2019 8;5(1):e000840. Epub 2019 Feb 8.

Department of Rheumatology, Leiden University Medical Center, Leiden, Netherlands.

Objectives: To complete reporting of outcomes after total withdrawal of all rheumatoid arthritis (RA) therapy and re-treatment after flare in Assessing Very Early Rheumatoid arthritis Treatment study (NCT01142726).

Methods: Patients with early RA were initially randomised to double-blind, weekly subcutaneous abatacept plus methotrexate, or abatacept or methotrexate monotherapy. At month 12, patients with Disease Activity Score (DAS)28 C reactive protein (CRP) <3. Read More

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http://dx.doi.org/10.1136/rmdopen-2018-000840DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6446179PMC
February 2019

Faceted polymersomes: a sphere-to-polyhedron shape transformation.

Chem Sci 2019 Mar 10;10(9):2725-2731. Epub 2019 Jan 10.

School of Chemistry , University of New South Wales , NSW 2052 , Australia . Email: ; Email:

The creation of "soft" deformable hollow polymeric nanoparticles with complex non-spherical shapes block copolymer self-assembly remains a challenge. In this work, we show that a perylene-bearing block copolymer can self-assemble into polymeric membrane sacs (polymersomes) that not only possess uncommonly faceted polyhedral shapes but are also intrinsically fluorescent. Here, we further reveal for the first time an experimental visualization of the entire polymersome faceting process. Read More

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http://xlink.rsc.org/?DOI=C8SC04206C
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http://dx.doi.org/10.1039/c8sc04206cDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6419931PMC
March 2019
1 Read

The effect of adding berry fruit juice concentrates and by-product extract to sugar solution on osmotic dehydration and sensory properties of apples.

J Food Sci Technol 2019 Apr 4;56(4):1927-1938. Epub 2019 Mar 4.

1Faculty of Food Sciences, Department of Food Engineering and Process Management, Warsaw University of Life Sciences (WULS - SGGW), 159c Nowoursynowska St., 02-776 Warsaw, Poland.

Osmotic dehydration (OD) of apples caused a reduction of normalized water content (NWC) and an increase of normalized solids gain (NSG), independently of the kind of osmotic solution. The use of 22°Brix osmotic solutions with the addition of fruit concentrates or bilberry extract resulted in only slight reduction in the NWC in the samples, i.e. Read More

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http://dx.doi.org/10.1007/s13197-019-03658-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6443819PMC

Targeting LIF-mediated paracrine interaction for pancreatic cancer therapy and monitoring.

Nature 2019 Apr 17. Epub 2019 Apr 17.

Molecular and Cell Biology Laboratory, Salk Institute for Biological Studies, La Jolla, CA, USA.

Pancreatic ductal adenocarcinoma (PDAC) has a dismal prognosis largely owing to inefficient diagnosis and tenacious drug resistance. Activation of pancreatic stellate cells (PSCs) and consequent development of dense stroma are prominent features accounting for this aggressive biology. The reciprocal interplay between PSCs and pancreatic cancer cells (PCCs) not only enhances tumour progression and metastasis but also sustains their own activation, facilitating a vicious cycle to exacerbate tumorigenesis and drug resistance. Read More

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http://www.nature.com/articles/s41586-019-1130-6
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http://dx.doi.org/10.1038/s41586-019-1130-6DOI Listing
April 2019
1 Read

Conformational plasticity in the KcsA potassium channel pore helix revealed by homo-FRET studies.

Sci Rep 2019 Apr 17;9(1):6215. Epub 2019 Apr 17.

CQFM-IN and iBB-Institute for Bioengineering and Bioscience, Instituto Superior Técnico, Universidade de Lisboa, 1049-001, Lisboa, Portugal.

Potassium channels selectivity filter (SF) conformation is modulated by several factors, including ion-protein and protein-protein interactions. Here, we investigate the SF dynamics of a single Trp mutant of the potassium channel KcsA (W67) using polarized time-resolved fluorescence measurements. For the first time, an analytical framework is reported to analyze the homo-Förster resonance energy transfer (homo-FRET) within a symmetric tetrameric protein with a square geometry. Read More

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http://www.nature.com/articles/s41598-019-42405-5
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http://dx.doi.org/10.1038/s41598-019-42405-5DOI Listing
April 2019
1 Read

Blood Lead Levels Among Resettled Refugee Children in Select US States, 2010-2014.

Pediatrics 2019 Apr 17. Epub 2019 Apr 17.

Immigrant, Refugee, and Migrant Health Branch, Division of Global Migration and Quarantine, Centers for Disease Control and Prevention, Atlanta, Georgia.

Background: Elevated blood lead levels (EBLLs; ≥5 µg/dL) are more prevalent among refugee children resettled in the United States than the general US population and contribute to permanent health and neurodevelopmental problems. The Centers for Disease Control and Prevention recommends screening of refugee children aged 6 months to 16 years on arrival in the United States and retesting those aged 6 months to 6 years between 3- and 6-months postarrival.

Methods: We analyzed EBLL prevalence among refugee children aged 6 months to 16 years who received a domestic refugee medical examination between January 1, 2010 and September 30, 2014. Read More

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http://dx.doi.org/10.1542/peds.2018-2591DOI Listing
April 2019
5.473 Impact Factor

Four new guaiane sesquiterpenoids from agarwood of Aquilaria filaria.

Fitoterapia 2019 Apr 14. Epub 2019 Apr 14.

Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, PR China. Electronic address:

Six 8,12-epoxyguaiane sesquiterpenes (1-6), together with two known 2-(2-phenylethyl)chromone derivatives (7-8) were isolated from agarwood originating from Aquilaria filaria. Their structures were established by spectroscopic methods including IR, HRESIMS, 1D, and 2D NMR, and comparison with the published data. The absolute configuration of compound 1 was unambiguously determined by quantum chemical calculation of the electronic circular dichroism (ECD) spectrum. Read More

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http://dx.doi.org/10.1016/j.fitote.2019.04.007DOI Listing

Pulsatile MEK Inhibition Improves Anti-tumor Immunity and T Cell Function in Murine Kras Mutant Lung Cancer.

Cell Rep 2019 Apr;27(3):806-819.e5

Ludwig Collaborative and Swim Across America Laboratory, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA; Parker Institute for Cancer Immunotherapy, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA; Department of Medicine, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA; Weill Cornell Medicine, New York, NY 10065, USA. Electronic address:

KRAS is one of the driver oncogenes in non-small-cell lung cancer (NSCLC) but remains refractory to current modalities of targeted pathway inhibition, which include inhibiting downstream kinase MEK to circumvent KRAS activation. Here, we show that pulsatile, rather than continuous, treatment with MEK inhibitors (MEKis) maintains T cell activation and enables their proliferation. Two MEKis, selumetinib and trametinib, induce T cell activation with increased CTLA-4 expression and, to a lesser extent, PD-1 expression on T cells in vivo after cyclical pulsatile MEKi treatment. Read More

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http://dx.doi.org/10.1016/j.celrep.2019.03.066DOI Listing

Phages Mediate Bacterial Self-Recognition.

Cell Rep 2019 Apr;27(3):737-749.e4

Department of Chemical Engineering, Pennsylvania State University, University Park, PA 16802-4400, USA. Electronic address:

Cells are social, and self-recognition is a conserved aspect of group behavior where cells assist kin and antagonize non-kin. However, the role of phage in self-recognition is unexplored. Here we find that a demarcation line is formed between different swimming Escherichia coli strains but not between identical clones; hence, motile cells discriminate between self and non-self. Read More

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http://dx.doi.org/10.1016/j.celrep.2019.03.070DOI Listing

Fluorescent nucleic acid probe in droplets for bacterial sorting (FNAP-sort) as a high-throughput screening method for environmental bacteria with various growth rates.

PLoS One 2019 17;14(4):e0214533. Epub 2019 Apr 17.

Department of Life Science and Medical Bioscience, Waseda University, Tokyo, Japan.

We have developed a new method for selectively sorting droplets containing growing bacteria using a fluorescence resonance energy transfer (FRET)-based RNA probe. Bacteria and the FRET-based RNA probe are encapsulated into nanoliter-scale droplets, which are incubated to allow for cell growth. The FRET-based RNA probe is cleaved by RNase derived from the bacteria propagated in the droplets, resulting in an increase in fluorescence intensity. Read More

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0214533PLOS

Harmonised collection of data in youth mental health: Towards large datasets.

Aust N Z J Psychiatry 2019 Apr 17:4867419844322. Epub 2019 Apr 17.

1 Orygen, The National Centre of Excellence in Youth Mental Health, Parkville, VIC, Australia.

Objective: The current international trend is to create large datasets with existing data and/or deposit newly collected data into repositories accessible to the scientific community. These practices lead to more efficient data sharing, better detection of small effects, modelling of confounders, establishment of sample generalizability and identification of differences between any given disorders. In Australia, to facilitate such data-sharing and collaborative opportunities, the Neurobiology in Youth Mental Health Partnership was created. Read More

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http://journals.sagepub.com/doi/10.1177/0004867419844322
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http://dx.doi.org/10.1177/0004867419844322DOI Listing
April 2019
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Serum S100A8/A9 as a Potentially Sensitive Biomarker for Inflammatory Bowel Disease.

Lab Med 2019 Apr 17. Epub 2019 Apr 17.

Faculty of Bioscience, Nagahama Institute of Bio-Science and Technology, Nagahama, Japan.

Background: The clinical significance of human S100A8/A9 (h-S100A8/A9) in patients with inflammatory bowel disease (IBD) is poorly understood.

Objective: To clarify whether serum S100A8/A9 is a sensitive biomarker for IBD.

Methods: Serum specimens from outpatients with IBD (n = 101) and healthy volunteers (HVs) (n = 101) were used in this study. Read More

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https://academic.oup.com/labmed/advance-article/doi/10.1093/
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http://dx.doi.org/10.1093/labmed/lmz003DOI Listing
April 2019
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Towards a molecular understanding of endosomal trafficking by Retromer and Retriever.

Traffic 2019 Apr 17. Epub 2019 Apr 17.

Institute for Molecular Bioscience, University of Queensland, St. Lucia, Queensland 4072, Australia.

Endosomes are dynamic intracellular compartments that control the sorting of a constant stream of different transmembrane cargos either for ESCRT-mediated degradation or for egress and recycling to compartments such as the Golgi and the plasma membrane. The recycling of cargos occurs within tubulovesicular membrane domains and is facilitated by peripheral membrane protein machineries that control both membrane remodelling and selection of specific transmembrane cargos. One of the primary sorting machineries is the Retromer complex, which controls the recycling of a large array of different cargo molecules in cooperation with various sorting nexin (SNX) adaptor proteins. Read More

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http://dx.doi.org/10.1111/tra.12649DOI Listing

Nitrogen and phosphorus retention in Danish restored wetlands.

Ambio 2019 Apr 16. Epub 2019 Apr 16.

Department of Bioscience, Aarhus University, Vejlsøvej 25, 8600, Silkeborg, Denmark.

Wetland restoration is considered an effective mitigation method for decreasing nitrogen (N) losses from agricultural land. However, when former cropland becomes rewetted, there is a risk that phosphorus (P) accumulated in soils will be released downstream. Here, we evaluate N and P retention in eight restored wetlands in Denmark monitored for 1 year using a mass balance approach. Read More

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http://link.springer.com/10.1007/s13280-019-01181-2
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http://dx.doi.org/10.1007/s13280-019-01181-2DOI Listing
April 2019
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Molecular phylogenetic species delimitation in the aquatic genus Ottelia (Hydrocharitaceae) reveals cryptic diversity within a widespread species.

J Plant Res 2019 Apr 16. Epub 2019 Apr 16.

Kadoorie Farm and Botanic Garden, Lam Kam Road, Tai Po, Hong Kong, People's Republic of China.

Ottelia, a pantropical genus of aquatic plants belonging to the family Hydrocharitaceae, includes several narrowly distributed taxa in Asia. Although the Asian species have received comparatively more research attention than congeners in other areas, various key taxonomic questions remain unaddressed, especially with regards to apparent cryptic diversity within O. alismoides, a widespread species complex native to Asia, northern Australia and tropical Africa. Read More

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http://dx.doi.org/10.1007/s10265-019-01109-7DOI Listing

Discovering novel mutation signatures by latent Dirichlet allocation with variational Bayes inference.

Bioinformatics 2019 Apr 16. Epub 2019 Apr 16.

Department of Electrical Engineering and Bioscience, Faculty of Science and Engineering, Waseda University.

Background: A cancer genome includes many mutations derived from various mutagens and mutational processes, leading to specific mutation patterns. It is known that each mutational process leads to characteristic mutations, and when a mutational process has preferences for mutations, this situation is called a "mutation signature." Identification of mutation signatures is an important task for elucidation of carcinogenic mechanisms. Read More

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http://dx.doi.org/10.1093/bioinformatics/btz266DOI Listing

Ranking network mechanisms by how they fit diverse experiments and deciding on 's ammonium transport and assimilation network.

NPJ Syst Biol Appl 2019 12;5:14. Epub 2019 Apr 12.

3Department of Molecular Cell Biology, Faculty of Science, VU University Amsterdam, O|2 building, Amsterdam, Netherlands.

The complex ammonium transport and assimilation network of involves the ammonium transporter AmtB, the regulatory proteins GlnK and GlnB, and the central N-assimilating enzymes together with their highly complex interactions. The engineering and modelling of such a complex network seem impossible because functioning depends critically on a gamut of data known at patchy accuracy. We developed a way out of this predicament, which employs: (i) a constrained optimization-based technology for the simultaneous fitting of models to heterogeneous experimental data sets gathered through diverse experimental set-ups, (ii) a 'rubber band method' to deal with different degrees of uncertainty, both in experimentally determined or estimated parameter values and in measured transient or steady-state variables (training data sets), (iii) integration of human expertise to decide on accuracies of both parameters and variables, (iv) massive computation employing a fast algorithm and a supercomputer, (v) an objective way of quantifying the plausibility of models, which makes it possible to decide which model is the best and how much better that model is than the others. Read More

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http://dx.doi.org/10.1038/s41540-019-0091-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6461619PMC

The in vitro host cell immune response to bovine-adapted Staphylococcus aureus varies according to bacterial lineage.

Sci Rep 2019 Apr 16;9(1):6134. Epub 2019 Apr 16.

Animal & Bioscience Department, Teagasc, Grange, Dunsany, Co. Meath, Ireland.

Mastitis is the most economically important disease affecting dairy cattle worldwide. Staphylococcus aureus is a highly prevalent cause of mastitis, causing infections ranging from sub-clinical to gangrenous. However, the interaction between the genotype of the infecting strain of S. Read More

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http://dx.doi.org/10.1038/s41598-019-42424-2DOI Listing

Primed histone demethylation regulates shoot regenerative competency.

Nat Commun 2019 Apr 16;10(1):1786. Epub 2019 Apr 16.

Faculty of Science and Technology, Department of Applied Biological Science, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba, 278-8510, Japan.

Acquisition of pluripotency by somatic cells is a striking process that enables multicellular organisms to regenerate organs. This process includes silencing of genes to erase original tissue memory and priming of additional cell type specification genes, which are then poised for activation by external signal inputs. Here, through analysis of genome-wide histone modifications and gene expression profiles, we show that a gene priming mechanism involving LYSINE-SPECIFIC DEMETHYLASE 1-LIKE 3 (LDL3) specifically eliminates H3K4me2 during formation of the intermediate pluripotent cell mass known as callus derived from Arabidopsis root cells. Read More

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http://www.nature.com/articles/s41467-019-09386-5
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http://dx.doi.org/10.1038/s41467-019-09386-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6467990PMC
April 2019
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Parents' Preferences and Perceptions of Their Children's Consumption of Sugar and Non-nutritive Sugar Substitutes.

Pediatr Dent 2019 Mar;41(2):119-128

Dr. Woods is a professor and a director, Division of Oral Diagnosis, Department of Diagnostic Sciences, College of Dentistry, University of Tennessee Health Science Center, Memphis.

The purpose of this study was to investigate opinions and preferences of parents from various sociodemographics regarding sugar and both natural and artificial non-nutritive sugar substitutes (NNS). Oral interview surveys were conducted in dental practice settings. Parents were asked about demographics, their consumption of and feelings about sugar and NNS for themselves and their children, and their concerns regarding NNS. Read More

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Thioredoxin H (TrxH) contributes to adversity adaptation and pathogenicity of Edwardsiella piscicida.

Vet Res 2019 Apr 15;50(1):26. Epub 2019 Apr 15.

Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, 571101, China.

Thioredoxins (Trxs) play an important role in defending against oxidative stress and keeping disulfide bonding correct to maintain protein function. Edwardsiella piscicida, a severe fish pathogen, has been shown to encode several thioredoxins including TrxA, TrxC, and TrxH, but their biological roles remain unknown. In this study, we characterized TrxH of E. Read More

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http://dx.doi.org/10.1186/s13567-019-0645-zDOI Listing