55,740 results match your criteria Bioscience of microbiota food and health[Journal]


Lactobacilli Have a Niche in the Human Nose.

Cell Rep 2020 May;31(8):107674

Department of Bioscience Engineering, University of Antwerp, Groenenborgerlaan 171, 2020 Antwerp, Belgium. Electronic address:

Although an increasing number of beneficial microbiome members are characterized for the human gut and vagina, beneficial microbes are underexplored for the human upper respiratory tract (URT). In this study, we demonstrate that taxa from the beneficial Lactobacillus genus complex are more prevalent in the healthy URT than in patients with chronic rhinosinusitis (CRS). Several URT-specific isolates are cultured, characterized, and further explored for their genetic and functional properties related to adaptation to the URT. Read More

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http://dx.doi.org/10.1016/j.celrep.2020.107674DOI Listing

A Comprehensive Integrated Anatomical and Molecular Atlas of Rat Intrinsic Cardiac Nervous System.

iScience 2020 May 23:101140. Epub 2020 May 23.

Daniel Baugh Institute for Functional Genomics/Computational Biology, Department of Pathology, Anatomy, and Cell Biology, Thomas Jefferson University, Philadelphia, PA, USA. Electronic address:

We have developed and integrated several technologies including whole-organ imaging and software development to support an initial precise 3D neuroanatomical mapping and molecular phenotyping of the intracardiac nervous system (ICN). While qualitative and gross anatomical descriptions of the anatomy of the ICN have each been pursued, we here bring forth a comprehensive atlas of the entire rat ICN at single-cell resolution. Our work precisely integrates anatomical and molecular data in the 3D digitally reconstructed whole heart with resolution at the micron scale. Read More

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http://dx.doi.org/10.1016/j.isci.2020.101140DOI Listing

Fetal -derived macrophages persist and sequentially maturate in ovaries after birth in mice.

Eur J Immunol 2020 May 27. Epub 2020 May 27.

Institute of Biomedicine, University of Turku, Turku, FI-20520, Finland.

Macrophages, which are highly diverse in different tissues, play a complex and vital role in tissue development, homeostasis and inflammation. The origin and heterogeneity of tissue-resident monocytes and macrophages in ovaries remains unknown. Here we identify three tissue-resident monocyte populations and five macrophage populations in the adult ovaries using high-dimensional single cell mass cytometry. Read More

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http://dx.doi.org/10.1002/eji.202048531DOI Listing

Diagnostic value of advanced semen analysis in evaluation of male infertility.

Andrologia 2020 May 26:e13625. Epub 2020 May 26.

American Center for Reproductive Medicine, Cleveland Clinic, Cleveland, OH, USA.

Conventional semen analysis is the standard of care to initially evaluate the fertility status of a male patient. However, it has some limitations and among these are failure to correctly identify the aetiology underlying fertility problems, intra- and inter-observer variability and incomplete information about sperm function. Considering these drawbacks, advanced semen tests have been developed to assess male infertility, including sperm function tests, oxidative stress (OS) and sperm DNA fragmentation (SDF) tests. Read More

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http://dx.doi.org/10.1111/and.13625DOI Listing

Novel Missense Variant in Heterozygous State in the Gene Leading to Intellectual Developmental Disorder With Dysmorphic Facies and Ptosis.

Front Genet 2020 7;11:368. Epub 2020 May 7.

Center of Excellence in Genomic Medicine Research, King Abdulaziz University, Jeddah, Saudi Arabia.

Intellectual developmental disorder with dysmorphic facies and ptosis is an autosomal dominant condition characterized by delayed psychomotor development, intellectual disability, delayed speech, and dysmorphic facial features, mostly ptosis. Heterozygous mutations in bromodomain and plant homeodomain (PHD) finger containing one ( gene have been reported. In this study, whole exome sequencing (WES) was performed as a molecular diagnostic test. Read More

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http://dx.doi.org/10.3389/fgene.2020.00368DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7221184PMC

Beclin 1 functions as a negative modulator of MLKL oligomerisation by integrating into the necrosome complex.

Cell Death Differ 2020 May 26. Epub 2020 May 26.

Department of Biochemistry, College of Life science and Biotechnology, Yonsei University, Seoul, Korea.

Necroptosis is a form of regulated cell death caused by formation of the necrosome complex. However, the factors modulating this process and the systemic pathophysiological effects of necroptosis are yet to be understood. Here, we identified that Beclin 1 functions as an anti-necroptosis factor by being recruited into the necrosome complex upon treatment with TNFα, Smac mimetic, and pan-caspase inhibitor and by repressing MLKL oligomerisation, thus preventing the disruption of the plasma membrane. Read More

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http://dx.doi.org/10.1038/s41418-020-0561-9DOI Listing

Streamlined human antibody generation and optimization by exploiting designed immunoglobulin loci in a B cell line.

Cell Mol Immunol 2020 May 26. Epub 2020 May 26.

Research Laboratories, Chiome Bioscience Inc, Tokyo, Japan.

Monoclonal antibodies (mAbs) are widely utilized as therapeutic drugs for various diseases, such as cancer, autoimmune diseases, and infectious diseases. Using the avian-derived B cell line DT40, we previously developed an antibody display technology, namely, the ADLib system, which rapidly generates antigen-specific mAbs. Here, we report the development of a human version of the ADLib system and showcase the streamlined generation and optimization of functional human mAbs. Read More

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http://dx.doi.org/10.1038/s41423-020-0440-9DOI Listing

Inhibition of mitochondrial complex I by the novel compound FSL0260 enhances high salinity-stress tolerance in Arabidopsis thaliana.

Sci Rep 2020 May 26;10(1):8691. Epub 2020 May 26.

Plant Genomic Network Research Team, RIKEN Center for Sustainable Resource Science (CSRS), Yokohama, 230-0045, Japan.

Chemical priming is an attractive and promising approach to improve abiotic stress tolerance in a broad variety of plant species. We screened the RIKEN Natural Products Depository (NPDepo) chemical library and identified a novel compound, FSL0260, enhancing salinity-stress tolerance in Arabidopsis thaliana and rice. Through transcriptome analysis using A. Read More

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http://dx.doi.org/10.1038/s41598-020-65614-9DOI Listing

A methyl-TROSY approach for NMR studies of high-molecular-weight DNA with application to the nucleosome core particle.

Proc Natl Acad Sci U S A 2020 May 26. Epub 2020 May 26.

Department of Molecular Genetics, The University of Toronto, Toronto, ON M5S 1A8, Canada;

The development of methyl-transverse relaxation-optimized spectroscopy (methyl-TROSY)-based NMR methods, in concert with robust strategies for incorporation of methyl-group probes of structure and dynamics into the protein of interest, has facilitated quantitative studies of high-molecular-weight protein complexes. Here we develop a one-pot in vitro reaction for producing NMR quantities of methyl-labeled DNA at the C5 and N6 positions of cytosine (5mC) and adenine (6mA) nucleobases, respectively, enabling the study of high-molecular-weight DNA molecules using TROSY approaches originally developed for protein applications. Our biosynthetic strategy exploits the large number of naturally available methyltransferases to specifically methylate DNA at a desired number of sites that serve as probes of structure and dynamics. Read More

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http://dx.doi.org/10.1073/pnas.2004317117DOI Listing

Obesity does not alter endometrial gene expression in women with endometriosis.

Reprod Biomed Online 2020 May 3. Epub 2020 May 3.

Department of Obstetrics and Gynhaecology, University of Melbourne and Gynaecology Research Centre, Level 7, Cnr Grattan St and Flemington Rd, Royal Women's Hospital, Parkville, Victoria 3052, Australia; Department of Anatomy, University of Otago, 270 Great King Street, Dunedin 9016, New Zealand.

Research Question: Does obesity affect endometrial gene expression in women with endometriosis, specifically women with stage I disease?

Design: Differential gene expression analysis was conducted on endometrium from women with and without endometriosis (n = 169). Women were diagnosed after surgical visualization and staged according to the revised American Society for Reproductive Medicine (stage I-IV). Women were grouped by body mass index (BMI) (kg/m) as underweight, normal, pre-obese or obese. Read More

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http://dx.doi.org/10.1016/j.rbmo.2020.03.015DOI Listing

The Gata1 murine megakaryocyte-erythroid progenitor cells expand robustly and alter differentiation potential.

Biochem Biophys Res Commun 2020 May 23. Epub 2020 May 23.

Immunotherapy Research Center, Korea Research Institute of Bioscience and Biotechnology Yuseong-gu, Daejeon, 34141, South Korea. Electronic address:

GATA1 is a master transcription factor of megakaryopoiesis and erythropoiesis, and loss-of-function mutation can induce accumulation of megakaryocyte-erythroid progenitors (MEPs) in mice and humans. Accordingly, the murine MEP cell line (termed G1ME2 cells) encoding doxycycline (dox)-inducible anti-Gata1 shRNA on Hprt locus has been developed. The cells were CD41CD71KIT, expand under dox, stem cell factor, and thrombopoietin (TPO), and terminally differentiate into erythroid cells or megakaryocytes upon removal of dox. Read More

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http://dx.doi.org/10.1016/j.bbrc.2020.04.143DOI Listing

Butylparaben Is Toxic to Porcine Oocyte Maturation and Subsequent Embryonic Development Following In Vitro Fertilization.

Int J Mol Sci 2020 May 24;21(10). Epub 2020 May 24.

Futuristic Animal Resource & Research Center, Korea Research Institute of Bioscience and Biotechnology, Chungcheongbuk-do 28116, Korea.

Parabens are widely used in personal care products due to their antimicrobial effects. Although the toxicity of parabens has been reported, little information is available on the toxicity of butylparaben (BP) on oocyte maturation. Therefore, we investigated the effects of various concentrations of BP (0 μM, 100 μM, 200 μM, 300 μM, 400 μM, and 500 μM) on the in vitro maturation of porcine oocytes. Read More

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http://dx.doi.org/10.3390/ijms21103692DOI Listing

MicrobioLink: An Integrated Computational Pipeline to Infer Functional Effects of Microbiome-Host Interactions.

Cells 2020 May 21;9(5). Epub 2020 May 21.

Earlham Institute, Norwich Research Park, Norwich NR4 7UZ, UK.

Microbiome-host interactions play significant roles in health and in various diseases including autoimmune disorders. Uncovering these inter-kingdom cross-talks propels our understanding of disease pathogenesis and provides useful leads on potential therapeutic targets. Despite the biological significance of microbe-host interactions, there is a big gap in understanding the downstream effects of these interactions on host processes. Read More

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http://dx.doi.org/10.3390/cells9051278DOI Listing

Phenotypic Heterogeneity and Plasticity of Cancer Cell Migration in a Pancreatic Tumor Three-Dimensional Culture Model.

Cancers (Basel) 2020 May 21;12(5). Epub 2020 May 21.

Department of Biomedicine & Health Sciences, Graduate School, The Catholic University of Korea, Seoul 06591, Korea.

Invasive cancer cell migration is a key feature of metastatic human pancreatic ductal adenocarcinoma (PDAC), yet the underlying mechanisms remain poorly understood. Here, we investigated modes of cancer cell invasion using two pancreatic cancer cell lines with differential epithelial-mesenchymal status, PANC-1 and BxPC-3, under 3D culture conditions. Multicellular tumor spheroids (TSs) were grown in a collagen matrix co-cultured with pancreatic stellate cells (PSCs) using microchannel chips. Read More

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http://dx.doi.org/10.3390/cancers12051305DOI Listing

ZNRD1 and Its Antisense Long Noncoding RNA ZNRD1-AS1 Are Oppositely Regulated by Cold Atmospheric Plasma in Breast Cancer Cells.

Oxid Med Cell Longev 2020 5;2020:9490567. Epub 2020 May 5.

Department of Life Science, Dongguk University-Seoul, Goyang 10326, Republic of Korea.

Cold atmospheric plasma (CAP) has been recognized as a potential alternative or supplementary cancer treatment tool, which is attributed by its selective antiproliferation effect on cancer cells over normal cells. Standardization of the CAP treatment in terms of biological outputs such as cell growth inhibition and gene expression change is essential for its clinical application. This study aims at identifying genes that show consistent expression profiles at a specific CAP condition, which could be used to monitor whether CAP is an appropriate treatment to biological targets. Read More

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http://dx.doi.org/10.1155/2020/9490567DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7225860PMC

Chromosome-Level Clam Genome Helps Elucidate the Molecular Basis of Adaptation to a Buried Lifestyle.

iScience 2020 May 11;23(6):101148. Epub 2020 May 11.

Jiangsu Key Laboratory of Marine Bioresources and Environment, Jiangsu Ocean University, Lianyungang 222005, China; Jiangsu Key Laboratory of Marine Biotechnology, Jiangsu Ocean University, Lianyungang 222005, China; Co-Innovation Center of Jiangsu Marine Bio-industry Technology, Jiangsu Ocean University, Lianyungang 222005, China. Electronic address:

Bivalve mollusks are economically important invertebrates that exhibit marked diversity in benthic lifestyle and provide valuable resources for understanding the molecular basis of adaptation to benthic life. In this report, we present a high-quality, chromosome-anchored reference genome of the Venus clam, Cyclina sinensis. The chromosome-level genome was assembled by Pacific Bioscience single-molecule real-time sequencing, Illumina paired-end sequencing, 10× Genomics, and high-throughput chromosome conformation capture technologies. Read More

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http://dx.doi.org/10.1016/j.isci.2020.101148DOI Listing

Salmonella intracellular adaptation is key to understand cephalosporin treatment relapse.

EBioMedicine 2020 May 23;56:102802. Epub 2020 May 23.

Quadram Institute Bioscience, Norwich, United Kingdom; University of East Anglia, Norwich, United Kingdom. Electronic address:

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http://dx.doi.org/10.1016/j.ebiom.2020.102802DOI Listing

Effects of low flow and co-occurring stressors on structural and functional characteristics of the benthic biofilm in small streams.

Sci Total Environ 2020 May 11;733:139331. Epub 2020 May 11.

Department of Biology, Aarhus University, Denmark.

Low flow and co-occurring stress is a more and more frequent phenomenon these years in small agricultural streams as a consequence of climate change. In the present study we explored short and longer term structural responses of the stream benthic algae community and biofilm metabolism to multiple stress in small streams applying a semi-experimental approach. We hypothesized that i) a reduction in flow in combination with secondary stress (nutrients and sediments) have immediate effects on the benthic algae community in terms of biomass (chlorophyll a, biovolume), taxonomic and trait (lifeform and size distribution) compositions as well as on metabolism (GPP and CR), and ii) that changes in the benthic algae community persist due to altered environmental settings but that functional redundancy among benthic algae species provides a high level of resilience in metabolism (GPP and CR). Read More

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http://dx.doi.org/10.1016/j.scitotenv.2020.139331DOI Listing

Effects of acute and chronic arecoline in adult zebrafish: Anxiolytic-like activity, elevated brain monoamines and the potential role of microglia.

Prog Neuropsychopharmacol Biol Psychiatry 2020 May 23:109977. Epub 2020 May 23.

School of Pharmacy, Southwest University, Chongqing, China; Ural Federal University, Ekaterinburg, Russia. Electronic address:

Arecoline is a naturally occurring psychoactive alkaloid with partial agonism at nicotinic and muscarinic acetylcholine receptors. Arecoline consumption is widespread, making it the fourth (after alcohol, nicotine and caffeine) most used substance by humans. However, the mechanisms of acute and chronic action of arecoline in-vivo remain poorly understood. Read More

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http://dx.doi.org/10.1016/j.pnpbp.2020.109977DOI Listing

Indonesian children fecal microbiome from birth until weaning was different from microbiomes of their mothers.

Gut Microbes 2020 May 26:1-19. Epub 2020 May 26.

Department of Microbiology & Immunology, Yong Loo Lin School of Medicine, National University of Singapore , Singapore.

Gastrointestinal (GI) microbiota play an important role in human health and wellbeing and the first wave of gut microbes arrives mostly through vertical transmission from mother to child. This study has undertaken to understand the microbiota profile of healthy Southeast Asian mother-infant pairs. Here, we examined the fecal, vaginal and breast milk microbiota of Indonesian mothers and the fecal microbiota of their children from less than 1 month to 48 months old. Read More

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http://dx.doi.org/10.1080/19490976.2020.1761240DOI Listing

Viral Hacks of the Plant Vasculature: The Role of Phloem Alterations in Systemic Virus Infection.

Annu Rev Virol 2020 May 26. Epub 2020 May 26.

Institute of Bioscience and Biotechnology Research, University of Maryland, College Park, Maryland 20742, USA; email:

For plant viruses, the ability to load into the vascular phloem and spread systemically within a host is an essential step in establishing a successful infection. However, access to the vascular phloem is highly regulated, representing a significant obstacle to virus loading, movement, and subsequent unloading into distal uninfected tissues. Recent studies indicate that during virus infection, phloem tissues are a source of significant transcriptional and translational alterations, with the number of virus-induced differentially expressed genes being four- to sixfold greater in phloem tissues than in surrounding nonphloem tissues. Read More

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http://dx.doi.org/10.1146/annurev-virology-010320-072410DOI Listing

Protein profiling in unlocking the basis of varicocele-associated infertility.

Andrologia 2020 May 26:e13645. Epub 2020 May 26.

American Center for Reproductive Medicine, Cleveland Clinic, Cleveland, OH, USA.

Varicocele is one of the major causes of male infertility and has a negative impact on spermatogenesis. The conventional semen analysis does not reveal the underlying subcellular mechanisms associated with defects in spermatozoa. Proteomics and bioinformatics analysis can be used to identify the molecular aetiologies associated with poor semen quality in varicocele patients. Read More

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http://dx.doi.org/10.1111/and.13645DOI Listing

Importance of stability analysis when using non-linear semi-mechanistic models to describe drug-induced haematotoxicity.

CPT Pharmacometrics Syst Pharmacol 2020 May 26. Epub 2020 May 26.

Clinical Pharmacology and Safety Sciences, BioPharmaceuticals R&D, AstraZeneca, Cambridge, UK.

Stability analysis, often overlooked in pharmacometrics, is essential to explore dynamical systems. The model developed by Friberg to describe drug-induced haematotoxicity is widely used to support decisions across drug development, and parameter values are often identified from observed blood counts. We use stability analysis to study the parametric dependence of stable and unstable solutions of several Friberg-type models, and highlight the risks associated with system instability in the context of non-linear mixed effects modelling. Read More

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http://dx.doi.org/10.1002/psp4.12514DOI Listing

Ovotesticular disorders of sex development (DSD) in FGF9 mouse models of human synostosis syndromes.

Hum Mol Genet 2020 May 26. Epub 2020 May 26.

Centre for Endocrinology and Metabolism, Hudson Institute of Medical Research, Molecular Genetics and Development Laboratory, Melbourne, 3168, Australia.

In mice, male sex determination depends on FGF9 signalling via FGFR2c in the bipotential gonads to maintain expression of the key testis gene SOX9. In humans however, while FGFR2 mutations have been linked to 46,XY disorders of sex development (DSD), the role of FGF9 is unresolved. The only reported pathogenic mutations in human FGF9; FGF9S99N and FGF9R62G, are dominant, and result in craniosynostosis (fusion of cranial sutures) or multiple synostoses (fusion of limb joints). Read More

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http://dx.doi.org/10.1093/hmg/ddaa100DOI Listing

Comparative transcriptomic analyses of Chromera and Symbiodiniaceae.

Environ Microbiol Rep 2020 May 25. Epub 2020 May 25.

ARC Centre of Excellence for Coral Reef Studies, James Cook University, Townsville, 4811, Queensland, Australia.

Reef-building corals live in a mutualistic relationship with photosynthetic algae (family Symbiodiniaceae) that usually provide most of the energy required by the coral host. This relationship is sensitive to temperature stress; as little as a 1°C increase often leads to collapse of the association. This sensitivity has led to interest in the potential of more stress tolerant algae to supplement or substitute for the normal Symbiodiniaceae mutualists. Read More

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http://dx.doi.org/10.1111/1758-2229.12859DOI Listing

Hyperpolarized [1- C] alanine production: A novel imaging biomarker of renal fibrosis.

Magn Reson Med 2020 May 25. Epub 2020 May 25.

MR Research Center, Department of Clinical Medicine, Aarhus University, Aarhus, Denmark.

Purpose: Renal tubulointerstitial fibrosis is strongly linked to the progressive decline of renal function seen in chronic kidney disease. State-of-the-art noninvasive diagnostic modalities are currently unable to detect the earliest changes associated with the onset of fibrosis. This study was undertaken to evaluate the potential for detecting the earliest alterations in fibrogenesis using a biofluid-based method and metabolic hyperpolarized [1- C]pyruvate imaging. Read More

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http://dx.doi.org/10.1002/mrm.28326DOI Listing

Setting maximum levels for lead in game meat in EC regulations: An adjunct to replacement of lead ammunition.

Ambio 2020 May 25. Epub 2020 May 25.

Department of Zoology, University of Cambridge, David Attenborough Building, Pembroke Street, Cambridge, CB2 3QZ, UK.

Each year, hunters from 12 of the 27 European Union (EU) countries and the UK shoot over 6 million large game mammals, 12 million rabbits and hares and over 80 million birds. They support an international game meat market worth over 1.1 thousand million Euros. Read More

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http://dx.doi.org/10.1007/s13280-020-01336-6DOI Listing

Superoxide-mediated oxidative stress accelerates skeletal muscle atrophy by synchronous activation of proteolytic systems.

Geroscience 2020 May 26. Epub 2020 May 26.

Oklahoma Medical Research Foundation, Oklahoma City, OK, USA.

The maintenance of skeletal muscle mass depends on the overall balance between the rates of protein synthesis and degradation. Thus, age-related muscle atrophy and function, commonly known as sarcopenia, may result from decreased protein synthesis, increased proteolysis, or simultaneous changes in both processes governed by complex multifactorial mechanisms. Growing evidence implicates oxidative stress and reactive oxygen species (ROS) as an essential regulator of proteolysis. Read More

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http://dx.doi.org/10.1007/s11357-020-00200-5DOI Listing

PGE1 and PGA1 bind to Nurr1 and activate its transcriptional function.

Nat Chem Biol 2020 May 25. Epub 2020 May 25.

Molecular Neurobiology Laboratory, Department of Psychiatry, McLean Hospital, Harvard Medical School, Belmont, MA, USA.

The orphan nuclear receptor Nurr1 is critical for the development, maintenance and protection of midbrain dopaminergic (mDA) neurons. Here we show that prostaglandin E1 (PGE1) and its dehydrated metabolite, PGA1, directly interact with the ligand-binding domain (LBD) of Nurr1 and stimulate its transcriptional function. We also report the crystallographic structure of Nurr1-LBD bound to PGA1 at 2. Read More

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http://dx.doi.org/10.1038/s41589-020-0553-6DOI Listing

Lineage-dependent gene expression programs influence the immune landscape of colorectal cancer.

Nat Genet 2020 May 25. Epub 2020 May 25.

Samsung Genome Institute, Samsung Medical Center, Seoul, Korea.

Immunotherapy for metastatic colorectal cancer is effective only for mismatch repair-deficient tumors with high microsatellite instability that demonstrate immune infiltration, suggesting that tumor cells can determine their immune microenvironment. To understand this cross-talk, we analyzed the transcriptome of 91,103 unsorted single cells from 23 Korean and 6 Belgian patients. Cancer cells displayed transcriptional features reminiscent of normal differentiation programs, and genetic alterations that apparently fostered immunosuppressive microenvironments directed by regulatory T cells, myofibroblasts and myeloid cells. Read More

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http://dx.doi.org/10.1038/s41588-020-0636-zDOI Listing

Resolvin D1 Ameliorates Nicotinamide-streptozotocin-induced Type 2 Diabetes Mellitus by its Anti-inflammatory Action and Modulating PI3K/Akt/mTOR Pathway in the Brain.

Arch Med Res 2020 May 22. Epub 2020 May 22.

BioScience Research Centre and Department of Medicine, Gayatri Vidya Parishad Medical College and Hospital, Visakhapatnam, India; UND Life Sciences, Battle Ground, Washington, USA. Electronic address:

Objective: To study whether resolvin D1 (RvD1), a metabolite of docosahexaenoic acid (DHA), prevents NA-STZ-induced type 2 diabetes mellitus (type 2 DM) in vivo and if so, what could be the mechanism of this action.

Material And Methods: Single intra-peritoneal (i.p) injection of NA-STZ (175 mg/kg body weight of NA and 65 mg/kg of STZ) was injected simultaneously with RvD1 (60 ng/animal) (injected for 5 consecutive days) to Wistar rats. Read More

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http://dx.doi.org/10.1016/j.arcmed.2020.05.002DOI Listing

Differential antimicrobial susceptibility profiles between symptomatic and asymptomatic non-typhoidal infections in Vietnamese children.

Epidemiol Infect 2020 May 26:1-9. Epub 2020 May 26.

Cambridge Institute of Therapeutic Immunology & Infectious Disease (CITIID), Cambridge Biomedical Campus, University of Cambridge, Cambridge, United Kingdom.

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http://dx.doi.org/10.1017/S0950268820001168DOI Listing

Healthy Eating Recommendations: Good for Reducing Dietary Contribution to the Body's Advanced Glycation/Lipoxidation End Products Pool?

Nutr Res Rev 2020 May 26:1-57. Epub 2020 May 26.

Department of Medicine, The Icahn School of Medicine at Mount Sinai, 1 Gustave L. Levy Pl, New York, NY 10029, USA.

This review aims to give dietary recommendations to reduce the occurrence of the Maillard reaction in foods and in vivo to reduce the body's AGE/ALE pool. A healthy diet, food reformulation and good culinary practices may be feasible for achieving the goal. A varied diet rich in fresh vegetables and fruits, non-added sugar beverages containing inhibitors of the Maillard reaction, and foods prepared by steaming and poaching as culinary techniques is recommended. Read More

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http://dx.doi.org/10.1017/S0954422420000141DOI Listing

Intermittent peripheral exposure to lipopolysaccharide induces exploratory behavior in mice and regulates brain glial activity in obese mice.

J Neuroinflammation 2020 May 25;17(1):163. Epub 2020 May 25.

Department of Life Sciences, College of Bioscience and Biotechnology, National Cheng Kung University, Tainan, Taiwan.

Background: Consecutive peripheral immune challenges can modulate the responses of brain resident microglia to stimuli. High-fat diet (HFD) intake has been reported to stimulate the activation of astrocytes and microglia in the arcuate nucleus (ARC) of the hypothalamus in obese rodents and humans. However, it is unknown whether intermittent exposure to additional peripheral immune challenge can modify HFD-induced hypothalamic glial activation in obese individuals. Read More

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http://dx.doi.org/10.1186/s12974-020-01837-xDOI Listing

Exposure of bay scallop Argopecten irradians to micro-polystyrene: Bioaccumulation and toxicity.

Comp Biochem Physiol C Toxicol Pharmacol 2020 May 22:108801. Epub 2020 May 22.

Marine Ecosystem and Biological Research Center, KIOST, Busan 49111, Republic of Korea.

Marine microplastic pollution poses a threat to aquatic organisms, including bivalves. In this study, we investigated the accumulation of microplastics and their elicited antioxidant stress response in the bay scallop Argopecten irradians. Scallops were exposed to 1 μm diameter micro-polystyrene (MP) beads at 10, 100, and 1000 beads/mL concentrations for a 7 day period. Read More

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http://dx.doi.org/10.1016/j.cbpc.2020.108801DOI Listing

Homeobox transcription factor OsZHD2 promotes root meristem activity in rice by inducing ethylene biosynthesis.

J Exp Bot 2020 May 25. Epub 2020 May 25.

Crop Biotech Institute and Graduate School of Biotechnology, Kyung Hee University, Yongin, Korea.

Root meristem activity is the most critical process influencing root development. Although several regulatory factors that regulate meristem activity have been identified in rice, studies on the enhancement of meristem activity in roots are limited. We identified a T-DNA activation tagging line of a zinc-finger homeobox gene, OsZHD2, which has longer seminal and lateral roots due to increased meristem activity. Read More

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http://dx.doi.org/10.1093/jxb/eraa209DOI Listing

TORC1 signaling regulates cytoplasmic pH through Sir2 in yeast.

Aging Cell 2020 May 25:e13151. Epub 2020 May 25.

Department of Microbiology and Molecular Biology, College of Bioscience and Biotechnology, Chungnam National University, Daejeon, Korea.

Glucose controls the phosphorylation of silent information regulator 2 (Sir2), a NAD -dependent protein deacetylase, which regulates the expression of the ATP-dependent proton pump Pma1 and replicative lifespan (RLS) in yeast. TORC1 signaling, which is a central regulator of cell growth and lifespan, is regulated by glucose as well as nitrogen sources. In this study, we demonstrate that TORC1 signaling controls Sir2 phosphorylation through casein kinase 2 (CK2) to regulate PMA1 expression and cytoplasmic pH (pHc) in yeast. Read More

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http://dx.doi.org/10.1111/acel.13151DOI Listing

Facile preparation of magnetic graphene oxide/nanoscale zerovalent iron adsorbent for magnetic solid-phase extraction of ultra-trace quinolones in milk samples.

Authors:
Dan Wei Ming Guo

J Sep Sci 2020 May 25. Epub 2020 May 25.

Center of Analysis and Testing, Zhejiang Research Institute of Chemical Industry Co., Ltd, Hangzhou, 310023, P. R. China.

A simple pH-responsive magnetic solid phase extraction method was developed using graphene oxide-coated nanoscale zerovalent iron nanoparticles as an efficient adsorbent prior to HPLC-MS/MS for determination of ultra-trace quinolones in milk samples. Various parameters affecting maghemite synthesis and separation such as pH of sample solution, amount of magnetic adsorbent, eluent type and volume were optimized. The limits of detection are from 3. Read More

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http://dx.doi.org/10.1002/jssc.202000108DOI Listing

Measuring Crop Motility and Food Passaging in Drosophila.

J Vis Exp 2020 May 9(159). Epub 2020 May 9.

Joint International Research Laboratory of Agriculture and Agri-Product Safety, the Ministry of Education of China, Yangzhou University; College of Bioscience and Biotechnology, Yangzhou University;

Most animals use the gastrointestinal (GI) tract to digest food. The movement of the ingested food in the GI tract is essential for nutrient absorption. Disordered GI motility and gastric emptying cause multiple diseases and symptoms. Read More

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http://dx.doi.org/10.3791/61181DOI Listing

Increased Cd biosorption capability of Aspergillus nidulans elicited by crpA deletion.

J Basic Microbiol 2020 May 25. Epub 2020 May 25.

Department of Molecular Biotechnology and Microbiology, Institute of Biotechnology, Faculty of Science and Technology, University of Debrecen, Debrecen, Hungary.

The P-type ATPase CrpA is an important Cu /Cd pump in the Aspergilli, significantly contributing to the heavy metal stress tolerance of these ascomycetous fungi. As expected, the deletion of crpA resulted in Cu /Cd -sensitive phenotypes in Aspergillus nidulans on stress agar plates inoculated with conidia. Nevertheless, paradoxical growth stimulations were observed with the ΔcrpA strain in both standard Cu stress agar plate experiments and cellophane colony harvest (CCH) cultures, when exposed to Cd . Read More

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http://dx.doi.org/10.1002/jobm.202000112DOI Listing

Secondary Pulmonary Alveolar Proteinosis Associated with Primary Myelofibrosis and Ruxolitinib Treatment: An Autopsy Case.

Intern Med 2020 May 23. Epub 2020 May 23.

Department of Hematology, Oncology and Respiratory Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Japan.

Pulmonary alveolar proteinosis (PAP) is an uncommon lung disorder characterized by the excessive accumulation of surfactant-derived lipoproteins in the pulmonary alveoli and terminal bronchiole. Secondary PAP associated with primary myelofibrosis (PMF) is extremely rare, and to our knowledge, no autopsy case has been reported. We herein report an autopsy case of secondary PAP occurring in a patient with PMF who was treated with the Janus kinase 1/2 inhibitor ruxolitinib. Read More

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http://dx.doi.org/10.2169/internalmedicine.4082-19DOI Listing

Endothelial Lipase Exerts its Anti-Atherogenic Effect through Increased Catabolism of β-VLDLs.

J Atheroscler Thromb 2020 May 23. Epub 2020 May 23.

Department of Molecular Pathology, Faculty of Medicine, Graduate School of Interdisciplinary Research, University of Yamanashi.

Aim: Endothelial lipase (EL) plays an important role in lipoprotein metabolism. Our recent study showed that increased hepatic expression of EL attenuates diet-induced hypercholesterolemia, thus subsequently reducing atherosclerosis in transgenic (Tg) rabbits. However, it is yet to be determined whether increased EL activity itself per se is anti-atherogenic or whether the anti-atherogenic effect of EL is exclusively dependent on its lipid-lowering effect. Read More

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http://dx.doi.org/10.5551/jat.55244DOI Listing

Clinical factors associated with progression to dementia in people with late-life depression: a cohort study of patients in secondary care.

BMJ Open 2020 May 24;10(5):e035147. Epub 2020 May 24.

King's College London, Institute of Psychiatry, Psychology and Neuroscience, London, UK.

Objectives: Depression can be a prodromal feature or a risk factor for dementia. We aimed to investigate which clinical factors in patients with late-life depression are associated with a higher risk of developing dementia and a more rapid conversion.

Design: Retrospective cohort study. Read More

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http://dx.doi.org/10.1136/bmjopen-2019-035147DOI Listing

Short communication: Menaquinone-4 (vitamin K) induces proliferation responses in bovine peripheral blood mononuclear cells.

J Dairy Sci 2020 May 21. Epub 2020 May 21.

Laboratory of Animal Breeding and Reproduction, Research Faculty of Agriculture, Hokkaido University, Kita-ku Kita 9 Nishi 9, Sapporo 060-8589, Japan; Global Station for Food, Land and Water Resources, Global Institution for Collaborative Research and Education, Hokkaido University, Sapporo 060-0815, Japan.

The effects of vitamin K (VK) on immune cells in ruminants are yet to be fully investigated. The objective of this study was to examine the effects of VK on peripheral blood mononuclear cells (PBMC) in Holstein dairy cows. A cell proliferation assay was performed to evaluate the effect of menaquinone-4 (MK-4, the biologically active form of VK) on immune response of PBMC. Read More

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http://dx.doi.org/10.3168/jds.2019-17987DOI Listing

Functional and structural characterization of a novel L-fucose mutarotase involved in non-phosphorylative pathway of L-fucose metabolism.

Biochem Biophys Res Commun 2020 May 21. Epub 2020 May 21.

Department of Bioscience, Graduate School of Agriculture, Ehime University, 3-5-7 Tarumi, Matsuyama, Ehime, 790-8566, Japan; Faculty of Agriculture, Ehime University, 3-5-7 Tarumi, Matsuyama, Ehime, 790-8566, Japan.

Mutarotases catalyze the α-β anomeric conversion of monosaccharide, and play a key role in utilizing sugar as enzymes involved in sugar metabolism have specificity for the α- or β-anomer. In spite of the sequential similarity to l-rhamnose mutarotase protein superfamily (COG3254: RhaM), the ACAV_RS08160 gene in Acidovorax avenae ATCC 19860 (AaFucM) is located in a gene cluster related to non-phosphorylative l-fucose and l-galactose metabolism, and transcriptionally induced by these carbon sources; therefore, the physiological role remains unclear. Here, we report that AaFucM possesses mutarotation activity only toward l-fucose by saturation difference (SD) NMR experiments. Read More

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http://dx.doi.org/10.1016/j.bbrc.2020.05.094DOI Listing

Identification and functional characterization of a novel acetyl-CoA carboxylase mutation associated with ketoenol resistance in Bemisia tabaci.

Pestic Biochem Physiol 2020 Jun 13;166:104583. Epub 2020 Apr 13.

Bayer AG, Crop Science Division, R&D, Pest Control, 40789 Monheim, Germany. Electronic address:

Insecticides of the tetronic/tetramic acid family (cyclic ketoenols) are widely used to control sucking pests such as whiteflies, aphids and mites. They act as inhibitors of acetyl-CoA carboxylase (ACC), a key enzyme for lipid biosynthesis across taxa. While it is well documented that plant ACCs targeted by herbicides have developed resistance associated with mutations at the carboxyltransferase (CT) domain, resistance to ketoenols in invertebrate pests has been previously associated either with metabolic resistance or with non-validated candidate mutations in different ACC domains. Read More

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http://dx.doi.org/10.1016/j.pestbp.2020.104583DOI Listing
June 2020
2.014 Impact Factor

Genomes of the dinoflagellate Polarella glacialis encode tandemly repeated single-exon genes with adaptive functions.

BMC Biol 2020 May 24;18(1):56. Epub 2020 May 24.

Institute for Molecular Bioscience, The University of Queensland, Brisbane, QLD, 4072, Australia.

Background: Dinoflagellates are taxonomically diverse and ecologically important phytoplankton that are ubiquitously present in marine and freshwater environments. Mostly photosynthetic, dinoflagellates provide the basis of aquatic primary production; most taxa are free-living, while some can form symbiotic and parasitic associations with other organisms. However, knowledge of the molecular mechanisms that underpin the adaptation of these organisms to diverse ecological niches is limited by the scarce availability of genomic data, partly due to their large genome sizes estimated up to 250 Gbp. Read More

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http://dx.doi.org/10.1186/s12915-020-00782-8DOI Listing

Structural and functional similarities and differences in nucleolar Pumilio RNA-binding proteins between Arabidopsis and the charophyte Chara corallina.

BMC Plant Biol 2020 May 24;20(1):230. Epub 2020 May 24.

Division of Bioscience and Bioinformatics, Myongji University, Yongin, Kyunggi-do, 449-728, South Korea.

Background: Pumilio RNA-binding proteins are evolutionarily conserved throughout eukaryotes and are involved in RNA decay, transport, and translation repression in the cytoplasm. Although a majority of Pumilio proteins function in the cytoplasm, two nucleolar forms have been reported to have a function in rRNA processing in Arabidopsis. The species of the genus Chara have been known to be most closely related to land plants, as they share several characteristics with modern Embryophyta. Read More

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http://dx.doi.org/10.1186/s12870-020-02444-xDOI Listing