2,498 results match your criteria Bioprocess And Biosystems Engineering[Journal]


Valorization of apple pomace using bio-based technology for the production of xylitol and 2G ethanol.

Bioprocess Biosyst Eng 2020 Jul 5. Epub 2020 Jul 5.

Lorena Engineering School, University of São Paulo, Estrada Municipal Do Campinho S/N, Lorena, São Paulo, CEP 12602-810, Brazil.

Apple pomace was studied as a raw material for the production of xylitol and 2G ethanol, since this agroindustrial residue has a high concentration of carbohydrate macromolecules, but is still poorly studied for the production of fermentation bioproducts, such as polyols. The dry biomass was subjected to dilute-acid hydrolysis with HSO to obtain the hemicellulosic hydrolysate, which was concentrated, detoxified and fermented. The hydrolyzate after characterization was submitted to submerged fermentations, which were carried out in Erlenmeyer flasks using, separately, the yeasts Candida guilliermondii and Kluyveromyces marxianus. Read More

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http://dx.doi.org/10.1007/s00449-020-02401-wDOI Listing

Effects of different carbon sources on 2,4,6-trichlorophenol degradation in the activated sludge process.

Bioprocess Biosyst Eng 2020 Jul 4. Epub 2020 Jul 4.

National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Beijing University of Technology, 100 Ping Le Yuan, Chaoyang District, Beijing, 100124, People's Republic of China.

The effects of different carbon sources on the enrichment of 2,4,6TCP-degrading microbes and on reactor stability were was investigated using a lab-scale sequencing batch reactor (SBR). Glucose, sucrose, and starch were selected as different carbon sources because of the different molecular weights. The sucrose-fed activated sludge (AS) exhibited faster adaption and higher degradation rates for 2,4,6-TCP in long-term operation and typical cycles compared to that fed with glucose and starch. Read More

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http://dx.doi.org/10.1007/s00449-020-02400-xDOI Listing

A promising approach toward efficient isolation of the exosomes by core-shell PCL-gelatin electrospun nanofibers.

Bioprocess Biosyst Eng 2020 Jul 1. Epub 2020 Jul 1.

Department of Industrial and Environmental Biotechnology, National Institute for Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran.

Exosomes as cell-derived vesicles are promising biomarkers for noninvasive and early detection of different types of cancer. However, a straightforward and cost-effective technique for isolation of exosomes in routine clinical settings is still challenging. Herein, we present for the first time, a novel coaxial nanofiber structure for the exosome isolation from body fluids with high efficiency. Read More

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http://dx.doi.org/10.1007/s00449-020-02385-7DOI Listing

Effective isolation of antioxidant Phelligridin LA from the fermentation broth of Inonotus baumii by macroporous resin.

Bioprocess Biosyst Eng 2020 Jun 30. Epub 2020 Jun 30.

Fujian Provincial University Engineering Research Center of Industrial Biocatalysis, College of Chemistry and Materials Science, Fujian Normal University, 32 Shangsan Road, Fuzhou, 350007, People's Republic of China.

Phelligridin LA (PLA) is a natural product with vigorous free radical scavenging activities accumulated in the liquid fermentation of herbal medicinal fungus Inonotus baumii. Aiming to establish an efficient isolation method of PLA from the fermentation broth, we evaluated the adsorption of PLA by macroporous resins. The best resin ADS-17 was screened for six candidates with various physical properties and adsorption behaviors. Read More

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http://dx.doi.org/10.1007/s00449-020-02398-2DOI Listing

Effect of cyclic and acyclic surfactants on the activity of Candida rugosa lipase.

Bioprocess Biosyst Eng 2020 Jun 29. Epub 2020 Jun 29.

Department of Chemistry, Selcuk University, 42075, Konya, Turkey.

The active site of Candida rugosa lipase (CRL) is mainly hydrophilic on its external face and hydrophobic on the internal side, and calix[n]arene-based surfactants form complexes with protein residues or with strong hydrogen bonds to open up the lid. Therefore, the activity of lipase persists for a long time. In this work, a series of cyclic and acyclic anionic surfactants (sodium dodecyl sulfate (SDS), p-sulfonatocalix[4]arene, and p-sulfonatocalix[8]arene) and zwitterionic surfactants (L-proline and L-proline derivative of calix[4]arene) were used to examine the relationship between the surfactants' molecular structures and their effects on the hydrolytic activity of CRL. Read More

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http://dx.doi.org/10.1007/s00449-020-02397-3DOI Listing

Surfactant removal from wastewater using photo-cathode microbial fuel cell and laterite-based hybrid treatment system.

Bioprocess Biosyst Eng 2020 Jun 28. Epub 2020 Jun 28.

Department of Civil Engineering, Indian Institute of Technology Kharagpur, Kharagpur, 721302, India.

Sodium dodecyl sulfate (SDS) is a widely used anionic surfactant, which finds its way to the receiving water body due to the incapability of conventional wastewater treatment systems to completely remove it. A hybrid treatment system consisting of upflow microbial fuel cell (MFC) with titanium dioxide (TiO) as a photocathode catalyst was developed for treating synthetic wastewater spiked with SDS (10.00 ± 0. Read More

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http://dx.doi.org/10.1007/s00449-020-02396-4DOI Listing

Dynamic modelling of growth and flavonoid production from Ocimum tenuiflorum suspension culture.

Bioprocess Biosyst Eng 2020 Jun 28. Epub 2020 Jun 28.

Department of Biotechnology, Karunya Institute of Technology and Sciences, Coimbatore, 641114, India.

A structured-segregated dynamic model for biomass growth, sucrose utilization and flavonoid production in Ocimum tenuiflorum suspension culture is proposed, considering a dynamic heterogeneous population of viable active, viable nonactive and dead cell. The sucrose hydrolysis (into glucose and fructose), substrate uptake by biomass and intracellular flavonoid production are modelled using Contois kinetics, a competitive double-substrate Monod, and Luedeking-Piret model, respectively. The conversion of active to viable-nonactive biomass has been formulated as a function of the total substrate and biomass concentrations. Read More

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http://dx.doi.org/10.1007/s00449-020-02394-6DOI Listing

Advanced nitrogen and phosphorus removal from municipal wastewater via simultaneous enhanced biological phosphorus removal and semi-nitritation (EBPR-SN) combined with anammox.

Bioprocess Biosyst Eng 2020 Jun 27. Epub 2020 Jun 27.

National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse TechnologyEngineering Research Center of Beijing, Beijing University of Technology, No.100, Ping Le Yuan, Chao Yang District, Beijing, 100124, China.

In this study, a novel laboratory-scale synchronous enhanced biological phosphorus removal and semi-nitritation (termed as EBPR-SN) combined with anammox process was put forward for achieving nutrient elimination from municipal wastewater at 27 ℃. This process consisted of two 10 L sequencing batch reactors (SBRs), i.e. Read More

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http://dx.doi.org/10.1007/s00449-020-02392-8DOI Listing

Ethyl esters production catalyzed by immobilized lipases is influenced by n-hexane and ter-amyl alcohol as organic solvents.

Bioprocess Biosyst Eng 2020 Jun 27. Epub 2020 Jun 27.

Department of Catalysis, CSIC/Campus UAM, 28049, Madrid, Spain.

Lipase stability in organic solvent is crucial for its application in many biotechnological processes as biocatalyst. One way to improve lipase's activity and stability in unusual reaction medium is its immobilization on inert supports. Here, lipases from different sources and immobilized through weak chemical interactions on hydrophobic and ionic supports had their transesterification ability dramatically dependent on the support and also on the solvent that had been used. Read More

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http://dx.doi.org/10.1007/s00449-020-02399-1DOI Listing

Bioprocess strategies for enhancing the outdoor production of Nannochloropsis gaditana: an evaluation of the effects of pH on culture performance in tubular photobioreactors.

Bioprocess Biosyst Eng 2020 Jun 25. Epub 2020 Jun 25.

Department of Chemical Engineering, University of Almería, 04120, Almería, Spain.

A priority of the industrial applications of microalgae is the reduction of production costs while maximizing algae biomass productivity. The purpose of this study was to carry out a comprehensive evaluation of the effects of pH control on the production of Nannochloropsis gaditana in tubular photobioreactors under external conditions while considering the environmental, biological, and operational parameters of the process. Experiments were carried out in 3. Read More

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http://dx.doi.org/10.1007/s00449-020-02373-xDOI Listing

Precise engineering of neutrophil membrane coated with polymeric nanoparticles concurrently absorbing of proinflammatory cytokines and endotoxins for management of sepsis.

Bioprocess Biosyst Eng 2020 Jun 24. Epub 2020 Jun 24.

Department of Critical Care Medicine, Wenzhou Central Hospital, No. 252, Baili East Road, Lucheng District, Wenzhou, 325000, China.

Sepsis, ensuing from unrestrained inflammatory replies to bacterial infections, endures with high injury and mortality worldwide. Presently, active sepsis management is missing in the hospitals during the surgery, and maintenance remnants mainly helpful. Now, we have constructed the macrophage bio-mimic nanoparticles for the treatment of sepsis and its management. Read More

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http://dx.doi.org/10.1007/s00449-020-02395-5DOI Listing

Enhanced volatile fatty acid production from sago hampas by Clostridium beijerinckii SR1 for bioelectricity generation using microbial fuel cells.

Bioprocess Biosyst Eng 2020 Jun 22. Epub 2020 Jun 22.

Department of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia.

Sago hampas is a starch-based biomass from sago processing industries consisted of 58% remaining starch. This study has demonstrated the bioconversion of sago hampas to volatile fatty acids (VFAs) by Clostridium beijerinckii SR1 via anaerobic digestion. Higher total VFAs were obtained from sago hampas (5. Read More

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http://dx.doi.org/10.1007/s00449-020-02391-9DOI Listing

Green and efficient biosynthesis of pectin-based copper nanoparticles and their antimicrobial activities.

Bioprocess Biosyst Eng 2020 Jun 22. Epub 2020 Jun 22.

Hunan Agricultural Product Processing Institute, Hunan Academy of Agricultural Sciences, Changsha, 410125, China.

Herein, we reported a green biosynthesis method of copper nanoparticles (CuNPs) at microwave irradiation condition by using pectin as a stabilizer and ascorbic acid as a reducing agent. Under the optimum conditions, CuNPs1 and 2 were synthesized under microwave times 0 and 3 min, respectively. Transmission electron microscope and scanning electron microscope (SEM) tests showed that CuNPs1 and 2 had irregular polygon particles with average diameters of 61. Read More

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http://dx.doi.org/10.1007/s00449-020-02390-wDOI Listing

Effects of methyl oleate and microparticle-enhanced cultivation on echinocandin B fermentation titer.

Bioprocess Biosyst Eng 2020 Jun 17. Epub 2020 Jun 17.

The National and Local Joint Engineering Research Center for Biomanufacturing of Chiral Chemicals, Zhejiang University of Technology, Hangzhou, 310014, People's Republic of China.

Echinocandin B (ECB) is a key precursor of antifungal agent Anidulafungin, which has demonstrated clinical efficacy in patients with invasive candidiasis. In this study, the effects of microparticle-enhanced cultivation and methyl oleate on echinocandin B fermentation titer were investigated. The results showed that the titer was significantly influenced by the morphological type of mycelium, and mycelium pellet was beneficial to improve the titer of this secondary metabolism. Read More

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http://dx.doi.org/10.1007/s00449-020-02389-3DOI Listing

Development of biocompatible DES/NADES as co-solvents for efficient biosynthesis of chiral alcohols.

Bioprocess Biosyst Eng 2020 Jun 11. Epub 2020 Jun 11.

College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou, 310032, People's Republic of China.

The novel deep eutectic solvents (DESs) and natural deep eutectic solvents (NADESs) were designed and synthesized by cell protective components, in which the compounds were derived from natural alternative sources. The performances of designed DESs/NADESs as co-solvent were investigated in asymmetric reduction catalyzed by microbial cells. The DESs/NADESs synthesized by three different types of hydrogen bond receptor (betaine, L-proline and L-carnitine) conferred an advantage over conventional choline chloride-based DESs/NADESs and aqueous buffer system, with regard to efficient bioproduction of (R)-1-[4-(trifluoromethyl)phenyl]ethanol by recombinant Escherichia coli cells. Read More

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http://dx.doi.org/10.1007/s00449-020-02387-5DOI Listing

Promoting enzymatic saccharification of organosolv-pretreated poplar sawdust by saponin-rich tea seed waste.

Bioprocess Biosyst Eng 2020 Jun 10. Epub 2020 Jun 10.

Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, College of Chemical Engineering, Nanjing Forestry University, Nanjing, 210037, People's Republic of China.

Organosolv pretreatment with two ethanol concentrations (25% and 50%, v/v) was performed to improve enzymatic saccharification of poplar sawdust. It was found that lower ethanol concentration (25%, v/v) pretreatment resulted in a higher enzymatic digestibility of poplar (38.1%) due to its higher xylan removal and similar lignin removal ratios, compared to that pretreated with 50% (v/v) ethanol pretreatment (27. Read More

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http://dx.doi.org/10.1007/s00449-020-02388-4DOI Listing

Efficient propagation of suspended HL-60 cells in a disposable bioreactor supporting wave-induced agitation at various Reynolds number.

Bioprocess Biosyst Eng 2020 Jun 9. Epub 2020 Jun 9.

Faculty of Chemical and Process Engineering, Warsaw University of Technology, Waryńskiego 1, 00-645, Warsaw, Poland.

Growth of human nonadherent HL-60 cell cultures performed in disposable bioreactor under various hydrodynamic conditions of 2-D wave-assisted agitation has been compared and discussed. Influence of Reynolds number for liquid (Re) and the ka coefficient, as key parameters characterized the bioprocessing of HL-60 cells in ReadyToProcess WAVE 25 system, on reached values of the apparent maximal specific growth rate (μ) and the specific yield of biomass (Y) has been identified. The values of Re (i. Read More

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http://dx.doi.org/10.1007/s00449-020-02386-6DOI Listing

The roles of extracellular polymeric substances of Pandoraea sp. XY-2 in the removal of tetracycline.

Bioprocess Biosyst Eng 2020 Jun 4. Epub 2020 Jun 4.

School of Minerals Processing and Bioengineering, Central South University, Changsha, 410083, China.

In this study, the roles of extracellular polymeric substances (EPSs) excreted by Pandoraea sp. XY-2 in the removal of tetracycline (TC) were investigated. In the early stage, TC in the solution was mainly removed by the adsorption of EPSs, which accounted for 20% of TC. Read More

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http://dx.doi.org/10.1007/s00449-020-02384-8DOI Listing

Separation of microbial oil produced by Mortierella isabellina using polymeric membranes.

Bioprocess Biosyst Eng 2020 May 30. Epub 2020 May 30.

Department of Chemical Engineering, Federal University of Santa Maria, Av. Roraima, 1000, Santa Maria, RS, 97105-900, Brazil.

The objective of this work was to concentrate, through a membrane separation process, the fatty acids from oil/solvent mixture. The oil was obtained by ultrasound-assisted extraction from freeze-dried cells of Mortierella isabellina. The concentration of the fatty acids was investigated using flat-sheet polymer membranes of ultrafiltration and nanofiltration. Read More

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http://dx.doi.org/10.1007/s00449-020-02383-9DOI Listing

Microbiome characterization and re-design by biologic agents for inflammatory bowel disease insights.

Bioprocess Biosyst Eng 2020 May 26. Epub 2020 May 26.

Department of Gastrointestinal Surgery, Zhujiang Hospital, Southern Medical University, GuangZhou, 510280, China.

The therapeutic effect of inflammatory bowel disease has improved in the past decades, but most of patients cannot tolerate, do not respond to drugs, or relapse after treating with conventional therapy. Therefore, new and more effective treatment methods are still needed in treatment of IBD. In this review, we will discuss the relevant mechanisms and the latest research progress of biologics (anti-TNF treatments, interleukin inhibitors, integrin inhibitors, antisense oligonucleotide, and JAK inhibitors) for IBD, focus on the efficacy and safety of drugs for moderate-to-severe IBD, and summarize the clinical status and future development direction of biologics in IBD. Read More

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http://dx.doi.org/10.1007/s00449-020-02380-yDOI Listing

Evaluation of analytical and numerical solutions of mass transfer model for breakthrough curves simulation: Co adsorption by hydrogen peroxide-bio-originated composite.

Bioprocess Biosyst Eng 2020 May 25. Epub 2020 May 25.

Department of Water Engineering, College of Agriculture, Shiraz University, 71365, Shiraz, Iran.

Accurate estimation of breakthrough curve (BTC) is required to scale -up the column adsorption process. A mathematical model (unsteady advection-dispersion-diffusion-adsorption equation) was solved analytically and numerically to simulate the dynamic adsorption of Co(II) ions on hydrogen peroxide-modified bone waste. The performance of both analytical and numerical approaches was evaluated under varying initial Co(II) concentrations (25, 50 and 75 mg L), bed heights (3, 6 and 9 cm), flow rates (0. Read More

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http://dx.doi.org/10.1007/s00449-020-02379-5DOI Listing

Selective adsorption of palladium and platinum from secondary wastewater using Escherichia coli BL21 and Providencia vermicola.

Bioprocess Biosyst Eng 2020 May 24. Epub 2020 May 24.

School of Minerals Processing and Bioengineering, Central South University, Changsha, 410083, People's Republic of China.

It is important to recover precious metals from secondary wastewater because of their low crustal abundance. The selective adsorption of palladium (Pd) and platinum (Pt) ions from secondary wastewater, which contains a large amount aluminium and sodium ions, was investigated using Escherichia coli BL21 (BL21), genetically modified E. coli BL21 (EC20) and Providencia vermicola (P. Read More

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http://dx.doi.org/10.1007/s00449-020-02378-6DOI Listing

Effect of graphene oxide with different morphological characteristics on properties of immobilized enzyme in the covalent method.

Bioprocess Biosyst Eng 2020 May 24. Epub 2020 May 24.

School of Chemistry and Chemical Engineering, Henan Polytechnic University, Jiaozuo, 454000, Henan, China.

Although graphene oxide (GO) has great potential in the field of immobilized enzyme catalysts, the detailed effects of GO with different morphological structures on immobilized enzyme are not well understood. GOs were prepared from 8000 mesh and nanoscale graphite at different reaction temperatures, and used as carriers to immobilize alpha-amylase by cross-linking method. The properties of GOs were characterized through Atomic force microscope, Fourier-transformed infrared, X-ray photoelectron spectroscopy, Raman and UV-Vis. Read More

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http://dx.doi.org/10.1007/s00449-020-02375-9DOI Listing
May 2020
1.997 Impact Factor

Mutant breeding of Starmerella bombicola by atmospheric and room-temperature plasma (ARTP) for improved production of specific or total sophorolipids.

Bioprocess Biosyst Eng 2020 May 24. Epub 2020 May 24.

School of Food and Biological Engineering, Hefei University of Technology, Hefei, 230009, China.

To enhance specific or total sophorolipids (SLs) production by Starmerella bombicola for specific application, mutant library consisting of 106 mutants from 7 batches was constructed via atmospheric and room-temperature plasma (ARTP). When compared to the wild strain, 11, 36 and 12 mutants performed increases over 30% in lactonic, acidic or total SLs production. Genetic stability investigation showed that 8, 7, and 4 mutants could maintain the improved SLs production capacity. Read More

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http://dx.doi.org/10.1007/s00449-020-02377-7DOI Listing

Improved protein purification system based on C-terminal cleavage of Npu DnaE split intein.

Bioprocess Biosyst Eng 2020 May 23. Epub 2020 May 23.

Department of Chemical and Biomolecular Engineering, Ohio State University, Columbus, OH, 43210, USA.

A purification system was constructed with the N-segment of the Npu DnaE split intein as an affinity ligand immobilized onto an epoxy-activated medium and the C-segment used as the cleavable tag fusing target protein. The affinity properties of C-tagged proteins adsorbed on I affinity chromatography medium were studied with GFP as a model target protein. The saturated adsorption capacity and dynamic adsorption capacity reached 51. Read More

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http://dx.doi.org/10.1007/s00449-020-02382-wDOI Listing

Organic wastes/by-products as alternative to CO for producing mixotrophic microalgae enhancing lipid production.

Bioprocess Biosyst Eng 2020 May 23. Epub 2020 May 23.

Gruppo Ricicla-DiSAA, Università Degli Studi Di Milano, Via Celoria 2, 20133, Milan, Italy.

In this work, white wine lees (WWL), cheese whey (CW), and glycerol (GLY) were used as carbon (C) sources to mixotrophically support the production of the microalga Nannochloropsis salina, replacing CO supply. In doing so, the alga was allowed to grow on C sources dosed at 2 g L, 3 g L, and 4 g L of C, in the presence and absence of CO supply. WWL and CW were not able to support the algal growth due to a fungal contamination that was genomically identified, while GLY gave interesting results in particular with 3 g L of C. Read More

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http://dx.doi.org/10.1007/s00449-020-02381-xDOI Listing

Enhanced nitrogen removal of aerobic denitrifier using extracellular algal organic matter as carbon source: application to actual reservoir water.

Bioprocess Biosyst Eng 2020 May 21. Epub 2020 May 21.

Key Laboratory of Northwest Water Resource, Environment and Ecology, MOE, Xi'an University of Architecture and Technology, Xi'an, 710055, People's Republic of China.

Man-made organic matter acting as carbon source for oligotrophic aerobic denitrification has been studied extensively, while less attention has been paid to the actual organic matter derived from drinking water reservoir. In this study, the effect of extracellular organic matter (EOM) released from Microcystis aeruginosa and Chlorella sp. and organic matter in actual reservoir water on aerobic denitrification performance of Acinetobacter johnsonii strain WGX-9 has been investigated, by measuring nitrogen removal and determining changes in the properties of organic matter. Read More

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http://dx.doi.org/10.1007/s00449-020-02376-8DOI Listing

Factors affecting simultaneous nitrification and denitrification (SND) in a moving bed sequencing batch reactor (MBSBR) system as revealed by microbial community structures.

Bioprocess Biosyst Eng 2020 May 20. Epub 2020 May 20.

College of Civil Engineering, Guangzhou University, 230 GuangZhou University City Outer Ring Road, Guangzhou, 510006, China.

The effects of biological factors including dissolved oxygen (DO), pH, carbon/nitrogen (C/N) and hydraulic retention times (HRT) on the performance of simultaneous nitrification and denitrification (SND) in a moving bed sequencing batch reactor (MBSBR) were investigated. A low DO was found to be advantageous to the SND in that nitrification was not inhibited, while pH and C/N ratio were shown to have positive effects on SND, and HRT needed to be controlled in a suitable range. A desirable SND efficiency was obtained at a DO of 2. Read More

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http://dx.doi.org/10.1007/s00449-020-02374-wDOI Listing

Biogenic FeS promotes dechlorination and thus de-cytotoxity of trichloroethylene.

Bioprocess Biosyst Eng 2020 May 18. Epub 2020 May 18.

School of Minerals Processing and Bioengineering, Central South University, Changsha, 410083, China.

Abiotic iron monosulfide (FeS) has attracted growing interests in dechlorinating trichloroethylene (TCE) in anoxic groundwater, but it is still unclear how biogenic FeS affects the dechlorination and thus the cytotoxity of TCE. In this work, a biogenic FeS was synthesized by Shewanella oneidensis MR-1 with addition of ferrihydrite and S, and it was used for dechlorination of TCE in alkaline environment and the de-cytotoxicity was evaluated by the growth of Synechocystis sp. PCC6803. Read More

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http://dx.doi.org/10.1007/s00449-020-02369-7DOI Listing

Highly efficient nitrate and phosphorus removal and adsorption of tetracycline by precipitation in a chitosan/polyvinyl alcohol immobilized bioreactor.

Bioprocess Biosyst Eng 2020 May 16. Epub 2020 May 16.

School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Xi'an, 710055, China.

Single denitrification using bacteria has been widely investigated, but few studies have focused on the simultaneous removal of nitrate, phosphorus. and tetracycline. Strain L2, an iron-reducing bacteria, was immobilized using chitosan/polyvinyl alcohol to simultaneously remove nitrate and phosphorus. Read More

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http://dx.doi.org/10.1007/s00449-020-02365-xDOI Listing

Production, partial purification and characterization of a proteoglycan bioemulsifier from an oleaginous yeast.

Bioprocess Biosyst Eng 2020 May 14. Epub 2020 May 14.

Department of Biotechnology, Indian Institute of Technology Kharagpur, West Bengal, Kharagpur, 721302, India.

In this study, Meyerozyma caribbica, an indigenously isolated oleaginous yeast, produced in media containing glucose a bioemulsifier that was partially characterized as a proteoglycan based on preliminary analysis. Optimization of carbon:nitrogen (C:N) ratio revealed 30:1 as the suitable ratio for enhanced production. Apart from higher emulsification activity (E: 70-80%), this molecule showed strong emulsion stability over a wide range of pH (2. Read More

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http://dx.doi.org/10.1007/s00449-020-02361-1DOI Listing

One-step utilization of inulin for docosahexaenoic acid (DHA) production by recombinant Aurantiochytrium sp. carrying Kluyveromyces marxianus inulinase.

Bioprocess Biosyst Eng 2020 May 13. Epub 2020 May 13.

Jiangsu Key Laboratory for Biomass-Based Energy and Enzyme Technology, Huaiyin Normal University, Huaian, 223300, People's Republic of China.

This study aimed to express an inulinase gene from the yeast Kluyveromyces marxianus (KmINU) in Aurantiochytrium sp. and realized one-step utilization of inulin resource for DHA production without any chemical pretreatment. An expression cassette with a length of 6052 bp for expressing the inulinase gene was constructed by a fast two-step PCR method and then was transferred into the Aurantiochytrium sp. Read More

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http://dx.doi.org/10.1007/s00449-020-02371-zDOI Listing

Repeated production of 6-(N-hydroxyethyl)-amino-6-deoxy-α-L-sorbofuranose by immobilized Gluconobacter oxydans cells with a strategy of in situ exhaustive cell regeneration.

Bioprocess Biosyst Eng 2020 May 12. Epub 2020 May 12.

Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, 310014, People's Republic of China.

The major troubles in 6-(N-hydroxyethyl)-amino-6-deoxy-α-L-sorbofuranose (6NSL) production from N-2-hydroxyethyl glucamine (NHEG) by Gluconobacter oxydans were low cell yield during cell preparation and loss of cells' biocatalytic ability during biotransformation, resulting in high production cost and low 6NSL production. The target of this work was to enhance 6NSL production by reusing cells and improving the cells biocatalytic ability. First, inhibitory effects of substrate and product on 6NSL production, and optimization of cell regeneration condition were investigated, respectively. Read More

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http://dx.doi.org/10.1007/s00449-020-02368-8DOI Listing

Metabolic engineering and optimization of the fermentation medium for vitamin B production in Escherichia coli.

Bioprocess Biosyst Eng 2020 May 12. Epub 2020 May 12.

Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, China.

Vitamin B is a crucial fine chemical that is widely used in the pharmaceutical, food and chemical industries, and its production solely dependents on microbial fermentation. We previously constructed an artificial vitamin B biosynthesis pathway in Escherichia coli, but the yield of the engineered strains was low. Here, we removed metabolic bottlenecks of the vitamin B biosynthesis pathway in engineered E. Read More

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http://dx.doi.org/10.1007/s00449-020-02355-zDOI Listing

Enhancement of ε-poly-L-lysine production in Streptomyces griseofuscus by addition of exogenous astaxanthin.

Bioprocess Biosyst Eng 2020 May 12. Epub 2020 May 12.

Marine College, Shandong University, Weihai, 264209, Shandong, China.

Addition of exogenous astaxanthin for improving ε-poly-L-lysine (ε-PL) production in Streptomyces griseofuscus was investigated in this study. By this unique strategy, the ε-PL production in shaker-flask fermentation was 2.48 g/L, which was 67. Read More

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http://dx.doi.org/10.1007/s00449-020-02372-yDOI Listing

Aging forming process of Chlorella vulgaris growing medium and its cultivation inhibition mechanism.

Bioprocess Biosyst Eng 2020 May 12. Epub 2020 May 12.

Department of Environmental Science and Engineering, School of Energy and Environment, Southeast University, No. 2 Sipailou Street, Nanjing, 210096, China.

To investigate the possibility of culture medium reuse in large-scale industrial microalgae cultivation for the alleviation of the massive water requirement pressure, the aging forming process of Chlorella vulgaris growing medium was explored and the aged medium's inhibition mechanisms on cell growth were inspected. The results demonstrated that when the medium was continuously reused, the collected maximal C. vulgaris biomass decreased. Read More

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http://dx.doi.org/10.1007/s00449-020-02370-0DOI Listing

Simultaneous liquefaction, saccharification, and fermentation of L-lactic acid using aging paddy rice with hull by an isolated thermotolerant Enterococcus faecalis DUT1805.

Bioprocess Biosyst Eng 2020 May 9. Epub 2020 May 9.

School of Bioengineering, Dalian University of Technology, No. 2 Linggong Road, Ganjingzi District, Dalian, 116024, Liaoning, P. R. China.

Simultaneous liquefaction, saccharification, and fermentation (SLSF) has attracted much attention for the production of bio-based chemicals, including L-lactic acid, due to its high efficiency and low cost. In this study, a lactic acid-producing bacterium with high tolerance of temperature up to 55 °C was isolated and characterized as Enterococcus faecalis DUT1805. Various strategies of stepwise controlled temperature were proposed and investigated for glucose utilization. Read More

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http://dx.doi.org/10.1007/s00449-020-02364-yDOI Listing

The effect of aeration and mixing in developing a dairy-based functional food rich in menaquinone-7.

Bioprocess Biosyst Eng 2020 May 7. Epub 2020 May 7.

School of Engineering, Faculty of Science and Engineering, The University of Waikato, Hamilton, 3216, New Zealand.

Vitamin menaquinone-7 (MK-7) supplementation improves bone health and reduces the incidence of osteoporosis. Despite the recent developments in MK-7 fermentation using Bacillus subtilis natto, low fermentation yields, as well as complicated downstream processing steps, are still the main reasons for the expensive final product. To overcome these issues, developing a fermented dairy-based product rich in MK-7 by avoiding costly downstream steps and optimising the fermentation operating conditions to enhance the MK-7 concentration would be an alternative approach. Read More

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http://dx.doi.org/10.1007/s00449-020-02366-wDOI Listing

A dual-parameter identification approach for data-based predictive modeling of hybrid gene regulatory network-growth kinetics in Pseudomonas putida mt-2.

Bioprocess Biosyst Eng 2020 May 6. Epub 2020 May 6.

Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology, Atlanta, GA, USA.

Data integration to model-based description of biological systems incorporating gene dynamics improves the performance of microbial systems. Bioprocess performance, typically predicted using empirical Monod-type models, is essential for a sustainable bioeconomy. To replace empirical models, we updated a hybrid gene regulatory network-growth kinetic model, predicting aromatic pollutants degradation and biomass growth in Pseudomonas putida mt-2. Read More

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http://dx.doi.org/10.1007/s00449-020-02360-2DOI Listing

Two-stage pH control combined with oxygen-enriched air strategies for the highly efficient production of EPA by Mortierella alpina CCFM698 with fed-batch fermentation.

Bioprocess Biosyst Eng 2020 May 6. Epub 2020 May 6.

State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, 214122, Jiangsu, P.R. China.

Dissolved oxygen and pH are critical factors influencing cell growth and metabolism. In our previous work, we constructed the recombinant strain Mortierella alpina CCFM698, which has the ability to produce EPA at room temperature. However, our experiments showed that the dissolved oxygen produced by the aeration and agitation of the fermenter was insufficient for cell growth and EPA synthesis by this recombinant strain. Read More

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http://dx.doi.org/10.1007/s00449-020-02367-9DOI Listing

Lipase-catalyzed synthesis of propyl-phenyl acetate: a kinetic and thermodynamic study.

Bioprocess Biosyst Eng 2020 May 4. Epub 2020 May 4.

Department of Chemical Engineering, Institute of Chemical Technology, Matunga (E), Mumbai, 400019, India.

This study focuses on the synthesis of propyl-phenyl acetate via esterification reaction in the presence of immobilized Candida antartica lipase-B (CAL-B). In this work, the effect of relevant factors (kinetics and thermodynamic) on total percent conversion and process optimization was studied. The reaction was performed in heptane medium with 1:2 molar ratio of benzoic acid: n-propanol with 0. Read More

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http://dx.doi.org/10.1007/s00449-020-02358-wDOI Listing
May 2020
1.997 Impact Factor

Anti-bacterial and anti-biofilm properties of green synthesized copper nanoparticles from Cardiospermum halicacabum leaf extract.

Bioprocess Biosyst Eng 2020 May 4. Epub 2020 May 4.

Electro-Materials Research Laboratory, Centre for Nanoscience and Technology, Pondicherry University, Puducherry, 605014, India.

In the present study, a copper nanoparticle (Cu NPs) was synthesized by a green synthesis method with Cardiospermum halicacabum leaf extract. The surface area of Cu NPs was measured with dynamic light scattering (DLS). UV-Vis spectrum clearly illustrates the typical absorption peak of Cu NPs. Read More

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http://dx.doi.org/10.1007/s00449-020-02357-xDOI Listing

Investigation of mitochondrial protein expression profiles of Yarrowia lipolytica in response to citric acid production.

Bioprocess Biosyst Eng 2020 May 3. Epub 2020 May 3.

Department of Biochemical Engineering, School of Chemistry, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, 21941-909, Brazil.

Nitrogen-limiting condition is essential for citric acid production by Yarrowia lipolytica. Mitochondrial protein expression profiles of Y. lipolytica IMUFRJ 50,682 cells cultivated in biomass proliferation medium (YPG medium, yeast extract, peptone and glycerol) and citric acid production medium (CA medium) were analyzed to identify differences in expressed proteins in response to medium composition. Read More

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http://dx.doi.org/10.1007/s00449-020-02363-zDOI Listing

Correction to: High lactobionic acid production by immobilized Zymomonas mobilis cells: a great step for large-scale process.

Bioprocess Biosyst Eng 2020 Jul;43(7):1277

Instituto de Biotecnologia, Universidade de Caxias Do Sul, PO Box 1352, Caxias do Sul, RS, 95001-970, Brazil.

In the original publication, table captions were incorrectly published. The correct captions are given here. Read More

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http://dx.doi.org/10.1007/s00449-020-02359-9DOI Listing

Bioreactor production of 2,3-butanediol by Pantoea agglomerans using soybean hull acid hydrolysate as substrate.

Bioprocess Biosyst Eng 2020 Apr 30. Epub 2020 Apr 30.

Biotechnology and Biochemical Engineering Laboratory (BiotecLab), Federal University of Rio Grande do Sul, Porto Alegre, Brazil.

Production of 2,3-butanediol (2,3-BD) by Pantoea agglomerans strain BL1 was investigated using soybean hull hydrolysate as substrate in batch reactors. The cultivation media consisted of a mixture of xylose, arabinose, and glucose, obtained from the hemicellulosic fraction of the soybean hull biomass. We evaluated the influence of oxygen supply, pH control, and media supplementation on the growth kinetics of the microorganism and on 2,3-BD production. Read More

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http://dx.doi.org/10.1007/s00449-020-02362-0DOI Listing

CO biofixation and fatty acid composition of two indigenous Dunaliella sp. isolates (ABRIINW-CH2 and ABRIINW-SH33) in response to extremely high CO levels.

Bioprocess Biosyst Eng 2020 Apr 30. Epub 2020 Apr 30.

Department of Food Biotechnology, Branch for Northwest and West Region, Agricultural Education and Extension Organization (AREEO), Agricultural Biotechnology Research Institute of Iran, Tabriz, Iran.

Global warming, as a result of atmospheric CO increase, is regarded as an important universal concern. Microalgae are considered as appropriate microorganisms for CO assimilation. Here we aimed to investigate carbon biofixation ability of two indigenous isolates of Dunaliella spp. Read More

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http://dx.doi.org/10.1007/s00449-020-02350-4DOI Listing

Development of an eco-friendly antibacterial textile: lysozyme immobilization on wool fabric.

Bioprocess Biosyst Eng 2020 Apr 29. Epub 2020 Apr 29.

Key Laboratory of Science and Technology of Eco-Textile, Ministry of Education, Jiangnan, University, Wuxi, 214122, Jiangsu, China.

Lysozyme, a type of natural enzyme, has been widely used for bacteriostatic functionalization of various materials due to its efficient and selective antibacterial properties. Herein, we report the preparation and characterization of an eco-friendly antibacterial textile based on the immobilization of lysozyme from chicken egg white onto wool fibers. Tris(hydroxymethyl)phosphine (THP) was employed as the cross-linker for the immobilization of lysozyme on the surface of wool fiber. Read More

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http://dx.doi.org/10.1007/s00449-020-02356-yDOI Listing

Heterologous expression of the novel α-helical hybrid peptide PR-FO in Bacillus subtilis.

Bioprocess Biosyst Eng 2020 Apr 29. Epub 2020 Apr 29.

Institute of Animal Nutrition, Northeast Agricultural University, No. 59 Mucai Street, Xiangfang District, Harbin, 150030, People's Republic of China.

PR-FO is a novel α-helical hybrid antimicrobial peptide (AMP) with strong antimicrobial activities and high stability, and the potential to develop into a new generation of antimicrobial agents. In this study, the encoded gene sequence of SMT3-PR-FO was designed and transformed into B. subtilis WB800N. Read More

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http://dx.doi.org/10.1007/s00449-020-02353-1DOI Listing

Microbial lipid production by oleaginous yeasts grown on Scenedesmus obtusiusculus microalgae biomass hydrolysate.

Bioprocess Biosyst Eng 2020 Apr 28. Epub 2020 Apr 28.

Werner Siemens-Lehrstuhl für Synthetische Biotechnologie, Technische Universität München, Garching, Germany.

Due to increasing oil prices and climate change concerns, biofuels have become increasingly important as potential alternative energy sources. However, the use of arable lands and valuable resources for the production of biofuel feedstock compromises food security and negatively affect the environment. Single cell oils (SCOs), accumulated by oleaginous yeasts, show great promise for efficient production of biofuels. Read More

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http://dx.doi.org/10.1007/s00449-020-02354-0DOI Listing

The seasonal changes of core bacterial community decide sewage purification in sub-plateau municipal sewage treatment plants.

Bioprocess Biosyst Eng 2020 Apr 24. Epub 2020 Apr 24.

School of Environmental and Municipal Engineering, Lanzhou Jiaotong University, Lanzhou, 730070, China.

The decline of sewage purification efficiency in winter is a frequent problem in sub-plateau municipal sewage treatment plants (MSTPs). Understanding the links between activated sludge (AS) bacterial community and sewage purification is crucial for exploring the cause of this problem. In this study, Illumina high-throughput sequencing technology was applied to investigate the seasonal changes of AS bacterial community in sub-plateau MSTPs. Read More

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http://dx.doi.org/10.1007/s00449-020-02352-2DOI Listing