2,231 results match your criteria Bioprocess And Biosystems Engineering[Journal]


Valorization of cheese whey and orange molasses for fungal biomass production by submerged fermentation with Rhizopus sp.

Bioprocess Biosyst Eng 2019 Apr 17. Epub 2019 Apr 17.

Food Research Division, AZTI-Tecnalia, Parque Tecnológico de Bizkaia, Astondo Bidea, Edf.609, 48160, Derio, Bizkaia, Spain.

Submerged fermentation (SmF) is an attractive biotechnological option for waste treatment, generating fungal bioprotein from food industry by-products. Using different Rhizopus sp. strains as fermentation agents, this paper describes a global strategy to identify interactions between cultivation parameters (pH 4. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00449-019-02127-4DOI Listing

Optimization of hyper-thermal acid hydrolysis and enzymatic saccharification of Ascophyllum nodosum for ethanol production with mannitol-adapted yeasts.

Bioprocess Biosyst Eng 2019 Apr 17. Epub 2019 Apr 17.

Department of Biotechnology, Pukyong National University, Busan, 608-737, Korea.

In this study, Ascophyllum nodosum was studied as a biomass for ethanol production. A. nodosum was degraded to monosaccharide by hyper-thermal (HT) acid hydrolysis and enzymatic saccharification and analyzed using response surface methodology (RSM) and the Michaelis-Menten equation. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00449-019-02123-8DOI Listing

Insights into the effect of imidazolium-based ionic liquids on chemical structure and hydrolytic activity of microbial lipase.

Bioprocess Biosyst Eng 2019 Apr 17. Epub 2019 Apr 17.

Department of Bioprocesses and Biotechnology, School of Pharmaceutical Sciences, São Paulo State University (UNESP), Rodovia Araraquara-Jaú/km 01, Araraquara, SP, 14800-903, Brazil.

This work studied the effect of the cation alkyl chain length of 1-alkyl-n-methylimidazolium chloride ([Cmim]Cl)-based ILs on the activity of Aspergillus niger lipase. First, the lipase activity in the presence of different ILs concentration over time was determined. ILs with shorter cation alkyl side chain length, namely [Cmim]Cl and [Cmim]Cl, promoted an increase of lipase activity; while, [Cmim]Cl, depending on its concentration, maintained or decreased the enzyme activity. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00449-019-02121-wDOI Listing

Effect of trophic conditions on microalga growth, nutrient removal, algal organic matter, and energy storage products in Scenedesmus (Acutodesmus) obliquus KGE-17 cultivation.

Bioprocess Biosyst Eng 2019 Apr 15. Epub 2019 Apr 15.

Department of Civil Engineering and Landscape Architecture, Suncheon Jeil College, Jeildaehak-gil 17, Suncheon, Cheonnam, 57997, South Korea.

This study compared the performance of microalga growth, nutrient removal, algal organic matter, and energy storage products in mixotrophic, photoautotrophic, and heterotrophic conditions. Scenedesmus obliquus was used as a model species. Mixotrophic condition showed the highest specific growth rate of 0. Read More

View Article

Download full-text PDF

Source
http://link.springer.com/10.1007/s00449-019-02120-x
Publisher Site
http://dx.doi.org/10.1007/s00449-019-02120-xDOI Listing
April 2019
1 Read

Production and recovery of poly-3-hydroxybutyrate bioplastics using agro-industrial residues of hemp hurd biomass.

Bioprocess Biosyst Eng 2019 Apr 16. Epub 2019 Apr 16.

Department of Chemical Engineering, Ryerson University, 350 Victoria St, Toronto, ON, M5B 2K3, Canada.

The present study describes production and recovery of poly(3-hydroxybutyrate) P(3HB) from agro-industrial residues. Production was conducted using Ralstonia eutropha strain with hemp hurd biomass hydrolysates sugars as a carbon source and ammonium chloride as the nitrogen source. Results show that maximum hydrolysis yield of 72. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00449-019-02109-6DOI Listing

Phospholipase D engineering for improving the biocatalytic synthesis of phosphatidylserine.

Bioprocess Biosyst Eng 2019 Apr 16. Epub 2019 Apr 16.

Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Pharmaceutical Sciences, Jiangnan University, Wuxi, 214122, People's Republic of China.

Phosphatidylserine is widely used in food, health, chemical and pharmaceutical industries. The phospholipase D-mediated green synthesis of phosphatidylserine has attracted substantial attention in recent years. In this study, the phospholipase D was heterologously expressed in Bacillus subtilis, Pichia pastoris, and Corynebacterium glutamicum, respectively. Read More

View Article

Download full-text PDF

Source
http://link.springer.com/10.1007/s00449-019-02116-7
Publisher Site
http://dx.doi.org/10.1007/s00449-019-02116-7DOI Listing
April 2019
2 Reads

Bioprocess development of a stable FUT8-CHO cell line to produce defucosylated anti-HER2 antibody.

Bioprocess Biosyst Eng 2019 Apr 13. Epub 2019 Apr 13.

Engineering Research Center of Cell and Therapeutic Antibody, Ministry of Education, School of Pharmacy, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai, 200240, People's Republic of China.

In recent years, an increasing number of defucosylated therapeutic antibodies have been applied in clinical practices due to their better efficacy compared to fucosylated counterparts. The establishment of stable and clonal manufacturing cell lines is the basis of therapeutic antibodies production. Bioprocess development of a new cell line is necessary for its future applications in the biopharmaceutical industry. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00449-019-02124-7DOI Listing

Removal of nitrogen by heterotrophic nitrification-aerobic denitrification of a novel halotolerant bacterium Pseudomonas mendocina TJPU04.

Bioprocess Biosyst Eng 2019 Apr 8. Epub 2019 Apr 8.

Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, College of Life Science, Nankai University, Tianjin, 300071, China.

Excess inorganic nitrogen in water poses a severe threat to enviroment. Removal of inorganic nitrogen by heterotrophic nitrifying-aerobic denitrifying microorganism is supposed to be a promising and applicable technology only if the removal rate can be maintained sufficiently high in real wastewater under various conditions, such as high concentration of salt and wide range of different nitrogen concentrations. Here, a new heterotrophic nitrifying-aerobic denitrifying bacterium was isolated and named as Pseudomonas mendocina TJPU04, which removes NH-N, NO-N and NO-N with average rate of 4. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00449-019-02088-8DOI Listing
April 2019
1 Read

Characterization of microbial community dynamics during the anaerobic co-digestion of thermally pre-treated slaughterhouse wastes with glycerin addition.

Bioprocess Biosyst Eng 2019 Apr 6. Epub 2019 Apr 6.

GIRO Program, Institute of Agrifood Research and Technology (IRTA), Torre Marimon, Caldes de Montbui, 08140, Barcelona, Spain.

Microbial community dynamics during the anaerobic co-digestion of pig manure, pasteurized slaughterhouse waste and glycerin were studied in a lab-scale CSTR. The feed composition was optimized through progressive co-substrate additions for enhanced methane production and organic matter removal without accumulation of intermediate compounds. Microbial community structure of biomass samples was studied by means of qPCR and DGGE profiling of 16S rRNA genes (Bacteria and Archaea), and genus-specific qPCR of the methyl coenzyme M reductase gene (mcrA), which encodes for an enzyme universally involved in methanogenesis. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00449-019-02115-8DOI Listing
April 2019
1 Read

Embedding live bacteria in porous hydrogel/ceramic nanocomposites for bioprocessing applications.

Bioprocess Biosyst Eng 2019 Apr 5. Epub 2019 Apr 5.

Keramische Werkstoffe und Bauteile, Advanced Ceramics, Universität Bremen, Am Biologischen Garten 2-IW 3, Raum 2140, 28359, Bremen, Germany.

In this work, we present a biocompatible one-pot processing route for ceramic/hydrogel nanocomposites in which we embed live bacteria. In our approach, we fabricate a highly stable alginate hydrogel with minimal shrinkage, highly increased structural and mechanical stability, as well as excellent biocompatibility. The hydrogel was produced by ionotropic gelation and reinforced with alumina nanoparticles to form a porous 3D network. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00449-019-02119-4DOI Listing
April 2019
2 Reads

Influence of the construction of porous spargers on lovastatin production by Aspergillus terreus ATCC 20,542 in a laboratory bubble column.

Bioprocess Biosyst Eng 2019 Apr 4. Epub 2019 Apr 4.

Univ Rennes, Ecole Nationale Supérieure de Chimie de Rennes, CNRS, ISCR-UMR 6226, 35000, Rennes, France.

In bubble column bioreactors, the hydrodynamic behavior like mixing time, bubble size and morphology of filamentous fungi are influenced by the construction of spargers. Sparger pore size is an important factor influencing formation of bubbles. In this study for the first time, a 5-L bubble column bioreactor with different porous spargers was used to investigate the effect of mean air bubble diameter (at 0. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00449-019-02118-5DOI Listing
April 2019
1 Read

Evaluation of predominant factor for shortcut biological nitrogen removal in sequencing batch reactor at ambient temperature.

Bioprocess Biosyst Eng 2019 Apr 3. Epub 2019 Apr 3.

R&D Center, Samsung Engineering Co., Ltd., 415-10 Woncheon-Dong, Youngtong-Gu, Suwon, Gyeonggi-Do, 443-823, Republic of Korea.

The shortcut biological nitrogen removal (SBNR) process requires less aeration and external carbon due to the oxidization of ammonia into nitrite and its direct denitrification to nitrogen gas during the biological nitrogen removal process. However, this process produces a poor effluent containing NH, since the system has to maintain a high free ammonia (FA, NH) concentration. To overcome this drawback, in this study, the solid retention time (SRT) and the dissolved oxygen (DO) concentration were controlled to achieve both a high ammonia removal rate and nitrite accumulation in the sequencing batch reactor (SBR) process, which can remove nitrogen from wastewater to the desired concentration and provide high free ammonia inhibition and continuous shock loading. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00449-019-02117-6DOI Listing
April 2019
1 Read

Biogas production: evaluation of the influence of KFeO pretreatment of maple leaves (Acer platanoides) on microbial consortia composition.

Bioprocess Biosyst Eng 2019 Apr 3. Epub 2019 Apr 3.

Institute of Molecular Biology, Slovak Academy of Science, Dúbravská cesta 21, 84551, Bratislava, Slovakia.

The potential of KFeO as a pretreatment agent of a lignocellulosic material was examined on leaves of Acer platanodides as the sole substrate for biogas production by anaerobic digestion carried out through modelling laboratory-scaled semi-continuous reactors differing in loading rates and substrate (pretreated and untreated leaves). The quality of bioagas produced by KFeO-pretreated leaves was significantly better in terms of higher methane content and lower content of HS. KFeO had no crucial influence on growth inhibition of biogas-producing bacteria, which were analysed by comprehensive culture-independent methods utilising high-throughput sequencing of specific genes [bacterial and archaeal 16S rRNA, formyltetrahydrofolate synthetase gene (fhs), methyl-coenzyme M reductase α subunit gene (mcrA) and fungal internal transcribed spacers (ITS)]. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00449-019-02112-xDOI Listing
April 2019
1 Read

Functionalized kaolin as support for endoglucanase immobilization.

Bioprocess Biosyst Eng 2019 Mar 29. Epub 2019 Mar 29.

Department of Chemical and Food Engineering, Federal University of Santa Catarina (UFSC), 88040-900, Florianópolis, SC, Brazil.

Endoglucanases are an enzyme of cellulases complex that has a great potential for many technological applications. One of the issues of its use concerns the recovery and reuse of this enzyme. Thus, in this study, the use of a surface-modified kaolin was evaluated to immobilize endoglucanase and evaluate the enzyme activity for its reuse. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00449-019-02113-wDOI Listing

Effects of sugarcane bagasse hydrolysate (SCBH) on cell growth and fatty acid accumulation of heterotrophic Chlorella protothecoides.

Bioprocess Biosyst Eng 2019 Mar 27. Epub 2019 Mar 27.

School of Food Science and Engineering, South China University of Technology, Guangzhou, 510641, People's Republic of China.

Microalgal lipid production by Chlorella protothecoides using sugarcane bagasse hydrolysate was investigated in this study. First, maximum glucose and reducing sugar concentrations of 15.2 and 27. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00449-019-02110-zDOI Listing

Coenzyme Q production by metabolic engineered Escherichia coli strains in defined medium.

Bioprocess Biosyst Eng 2019 Mar 26. Epub 2019 Mar 26.

Biochemical Engineering School, Pontificia Universidad Católica de Valparaíso, Av. Brasil 2085, Casilla 4059,, Valparaíso, Chile.

Coenzyme Q (CoQ) plays an important role as an electron transporter in the respiratory chain. It is formed from a benzoquinone ring and an isoprenoid chain of a specific length depending on the organism. We constructed an engineered Escherichia coli strain (menF) unable to produce demethylmenaquinone and menaquinone, compounds that compete for both chorismate, precursor of the benzoquinone ring, and the isoprenoid chain involved in CoQ biosynthesis. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00449-019-02111-yDOI Listing

Impact of phenol on the performance, kinetics, microbial communities and functional genes of an autotrophic denitrification system.

Bioprocess Biosyst Eng 2019 Mar 25. Epub 2019 Mar 25.

College of Urban Construction, Nanjing Tech University, Nanjing, 211816, China.

Nitrate and phenol often co-occur in wastewater because of the complex industrial and agricultural processes, while the impacts of phenol on autotrophic denitrification remain unclear. Here, a sulfur and hydrogen-oxidizing autotrophic denitrification reactor was established, and the effects of different concentrations of phenol on the nitrate removal performance, kinetics, microbial communities, and functional genes were investigated. Increasing concentrations of phenol significantly decreased the denitrification efficiency in the reactor. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00449-019-02108-7DOI Listing
March 2019
1 Read

Improving cellulosic ethanol fermentation efficiency by converting endogenous water-soluble carbohydrates into citric acid before pretreatment.

Bioprocess Biosyst Eng 2019 Mar 25. Epub 2019 Mar 25.

State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237, China.

Water-soluble carbohydrates in raw lignocellulosic biomass are converted into hydroxymethylfurfural (HMF) in the acid-based pretreatment, thus increasing the detoxification intensity and reducing the fermentation efficiency of cellulosic ethanol. Therefore, reducing water-soluble carbohydrates in raw corn stover is crucially important to reduce the inhibitors' generation and improve the ethanol fermentation efficiency. In this study, aerobic solid-state fermentation of corn stover by inoculating Aspergillus niger spores converted 83% of the endogenous water-soluble carbohydrates into citric acid, leading to the decrease of 41% of HMF generation and 8% of sulfuric acid usage during the dry acid pretreatment. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00449-019-02107-8DOI Listing

Characterization of pyrene and chrysene degradation by halophilic Hortaea sp. B15.

Bioprocess Biosyst Eng 2019 Mar 19. Epub 2019 Mar 19.

Botany and Microbiology Department, College of Sciences, King Saud University, P.O. Box 22452, Riyadh, 11495, Saudi Arabia.

Polycyclic aromatics hydrocarbons (PAHs) are ubiquitous and toxic pollutants that are dangerous to humans and living organism in aquatic environment. Normally, PAHs has lower molecular weight such as phenanthrene and naphthalene that are easy and efficient to degrade, but high-molecular-weight PAHs such as chrysene and pyrene are difficult to be biodegraded by common microorganism. This study investigated the isolation and characterization of a potential halophilic bacterium capable of utilizing two high-molecular-weight PAHs. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00449-019-02096-8DOI Listing

Precipitation of complex antibody solutions: influence of contaminant composition and cell culture medium on the precipitation behavior.

Bioprocess Biosyst Eng 2019 Mar 18. Epub 2019 Mar 18.

Institute of Process Engineering in Life Sciences, Section IV: Biomolecular Separation Engineering, Karlsruhe Institute of Technology (KIT), 76131, Karlsruhe, Germany.

Preparative protein precipitation is known as a cost-efficient and easy-to-use alternative to chromatographic purification steps. This said, at the moment, there is no process for monoclonal antibodies (mAb) on the market, although especially polyethylene glycol-induced precipitation has shown great potential. One reason might be the highly complex behavior of each component of a crude feedstock during the precipitation process. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00449-019-02103-yDOI Listing
March 2019
3 Reads

Enhancing the production of tacrolimus by engineering target genes identified in important primary and secondary metabolic pathways and feeding exogenous precursors.

Bioprocess Biosyst Eng 2019 Mar 18. Epub 2019 Mar 18.

Key Laboratory of Systems Bioengineering (Ministry of Education), Tianjin University, Tianjin, 300072, People's Republic of China.

Tacrolimus has been widely used as a powerful novel immunosuppressant. The objective of this study was to improve the production of tacrolimus by engineering the target genes of important primary and secondary metabolic pathways and feeding exogenous precursors. Based on the metabonomics analysis, the shikimic acid pathway is an important primary metabolic pathway for the producing tacrolimus. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00449-019-02106-9DOI Listing
March 2019
6 Reads
1.997 Impact Factor

Integrated thermophilic enzyme-immobilized reactor and high-rate biological reactors for treatment of palm oil-containing wastewater without sludge production.

Bioprocess Biosyst Eng 2019 Mar 16. Epub 2019 Mar 16.

Environmental Science and Engineering, CSIR-Central Leather Research Institute, Adyar, Chennai, 600 020, India.

Bacillus pumilus, the thermophilic microorganism, was used to biosynthesise lipase of specific activity 5173 U. The purified lipase was highly stable in the pH range from 1 to 7 and temperature at 50 °C. The functionalized nanoporous activated carbon matrix was used for the immobilization of lipase at the optimum conditions and was used for the hydrolysis of palm oil-containing wastewater at optimum time, 3 h, pH 7, and at temperature 50 °C. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00449-019-02104-xDOI Listing

Positional orientating co-immobilization of bienzyme CPO/GOx on mesoporous TiO thin film for efficient cascade reaction.

Bioprocess Biosyst Eng 2019 Mar 16. Epub 2019 Mar 16.

School of Chemistry and Chemical Engineering, Shaanxi Normal University, No. 620 West Chang'an Road, Chang'an District, Xi'an, 710119, People's Republic of China.

A multitude of industrial processes are catalyzed by two or more enzymes working together in a cascade way. However, designing efficient enzymatic cascade reactions is still a challenge. In this work, a TiO thin film with mesoporous pores was prepared and used as carrier for co-immobilization of chloroperoxidase (CPO) and glucose peroxidase (GOx). Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00449-019-02105-wDOI Listing
March 2019
1 Read

Application of process system engineering tools to the fed-batch production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) from a vinasses-molasses Mixture.

Bioprocess Biosyst Eng 2019 Mar 14. Epub 2019 Mar 14.

SIDCOP Research Group, University of Antioquia, Medellín, Colombia.

Fed-batch production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) copolymer using vinasses-molasses mixture is carried out in this work by implementing different process systems engineering tools. Two fed-batch strategies are tested experimentally at 5 L scale, considering only offline information: (1) offline optimizing control and (2) exponential feeding. Application of these strategies showed that different feeding profiles result in different dynamic behaviour, influencing both, yield and biopolymer properties. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00449-019-02102-zDOI Listing

Correction to: Voltammetric detection of anti-HIV replication drug based on novel nanocomposite gold-nanoparticle-CaCO hybrid material.

Bioprocess Biosyst Eng 2019 Mar 11. Epub 2019 Mar 11.

Department of Biochemistry, M. D. University, Rohtak, 124001, Haryana, India.

The Figs. 2, 4 and 5 were published wrongly in this paper, due to inadvertent compilation of figures during uploading the paper. Read More

View Article

Download full-text PDF

Source
http://link.springer.com/10.1007/s00449-019-02097-7
Publisher Site
http://dx.doi.org/10.1007/s00449-019-02097-7DOI Listing
March 2019
3 Reads

Effect of aeration and agitation on yeast inulinase production: a biocalorimetric investigation.

Bioprocess Biosyst Eng 2019 Mar 11. Epub 2019 Mar 11.

Thermochemical Lab, Chemical Engineering Department, Central Leather Research Institute (CSIR-CLRI), Adyar, Chennai, Tamilnadu, 600020, India.

Air flow rate and agitation speed for inulinase production by Kluyveromyces marxianus were optimized based on metabolic heat release profiles. Shear stress and oxygen transfer (ka) values were compared to assess the effects of aeration and agitation. At agitation rates of ≤ 100 rpm, the oxygen mass transfer rates were small and eventually led to less inulinase production, but at agitation rates > 150 rpm, loss of biomass resulted in less inulinase activity. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00449-019-02101-0DOI Listing
March 2019
1 Read

Highly efficient synthesis of boldenone from androst-4-ene-3,17-dione by Arthrobacter simplex and Pichia pastoris ordered biotransformation.

Bioprocess Biosyst Eng 2019 Mar 8. Epub 2019 Mar 8.

Key Laboratory of Industrial Fermentation Microbiology (Tianjin University of Science and Technology), Ministry of Education, Tianjin Key Laboratory of Industrial Microbiology, Tianjin Engineering Research Center of Microbial Metabolism and Fermentation Process Control, College of Biotechnology, Tianjin University of Science and Technology, Tianjin, 300457, People's Republic of China.

Boldenone (BD) is an important steroid hormone drug which is the derivative of testosterone. In this study, an ordered biotransformation method was proposed employing Arthrobacter simplex and recombinant Pichia pastoris with 17β-hydroxysteroid dehydrogenase from Saccharomyces cerevisiae to produce BD from androst-4-ene-3,17-dione (AD) efficiently. To lower the oxidation towards BD in A. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00449-019-02092-yDOI Listing
March 2019
1 Read

Average shear rate in airlift bioreactors: searching for the true value.

Bioprocess Biosyst Eng 2019 Mar 8. Epub 2019 Mar 8.

Graduate Program of Chemical Engineering, Federal University of São Carlos, C.P. 676, São Carlos, SP, 13565-905, Brazil.

The shear rate is an important bioreactor parameter that needs to be evaluated due to its impact on microorganism morphology and viability, and consequently on bioproduct formation. Airlift bioreactors, classified as low-shear devices, are used as an alternative to conventional stirred-tank reactors. Considerable efforts have been made to characterize the shear environments in airlift bioreactors, using the average shear rate ([Formula: see text]) as a key parameter. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00449-019-02100-1DOI Listing

Microalga Scenedesmus bajacalifornicus BBKLP-07, a new source of bioactive compounds with in vitro pharmacological applications.

Bioprocess Biosyst Eng 2019 Mar 7. Epub 2019 Mar 7.

Department of Studies in Biotechnology and Microbiology, Karnataka University, 580003, Dharwad, India.

Microalgae are photosynthetic eukaryotes which are primary producers in the food chain and also excellent sources for bioactive compounds such as alkaloids, flavonoids, phenols, saponins and other fine chemicals. In the present study, the microalga Scenedesmus bajacalifornicus BBKLP-07 was subjected to soxhlet extraction using solvents like chloroform, acetone, ethanol, methanol and aqueous solvents. All the solvents were tested for the presence of phytochemical constituents such as alkaloids, flavonoids, glycosides, phenols, lignin's, saponins, sterols, tannins, anthraquinone and reducing sugar using the standard procedures. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00449-019-02099-5DOI Listing

The potential of a natural biopolymeric flocculant, ε-poly-L-lysine, for harvesting Chlorella ellipsoidea and its sustainability perspectives for cost and toxicity.

Bioprocess Biosyst Eng 2019 Mar 4. Epub 2019 Mar 4.

Biomass Research Group, Gyeongnam Department of Environmental Toxicology and Chemistry, Korea Institute of Toxicology, 17, Jegok-gil, Samgok-ri, Munsan-eup, Jinju-si, Gyeongsangnam- do, 52834, Republic of Korea.

The successful production of microalgal biomass requires the precise coordination of many different steps. Cell harvesting is a central process in all methods currently used for the production of microalgal biomass. Therefore, improving the harvesting process itself, and using a harvesting method that is compatible with adjacent steps, is necessary to prevent problems that may occur during downstream processing. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00449-019-02098-6DOI Listing

Hydrolyzed polyacrylamide biotransformation in an up-flow anaerobic sludge blanket reactor system: key enzymes, functional microorganisms, and biodegradation mechanisms.

Bioprocess Biosyst Eng 2019 Feb 28. Epub 2019 Feb 28.

College of Chemistry and Chemical Engineering, Ocean University of China, Qingdao, 266100, China.

Hydrolyzed polyacrylamide (HPAM) biotransformation in an up-flow anaerobic sludge blanket reactor including biodegradation performances, biodegradation mechanisms, key enzymes, and functional microorganisms was explored. Response surface methodology was applied to further improve HPAM degradation. The predicted degradation ratios of HPAM and COD were 46. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00449-019-02094-wDOI Listing
February 2019
2 Reads

Enhanced ethanol production from industrial lignocellulose hydrolysates by a hydrolysate-cofermenting Saccharomyces cerevisiae strain.

Bioprocess Biosyst Eng 2019 Feb 28. Epub 2019 Feb 28.

School of Life Sciences and Chemical Technology, Ngee Ann Polytechnic, 535 Clementi Road, Singapore, 599489, Singapore.

Industrial production of lignocellulosic ethanol requires a microorganism utilizing both hexose and pentose, and tolerating inhibitors. In this study, a hydrolysate-cofermenting Saccharomyces cerevisiae strain was obtained through one step in vivo DNA assembly of pentose-metabolizing pathway genes, followed by consecutive adaptive evolution in pentose media containing acetic acid, and direct screening in biomass hydrolysate media. The strain was able to coferment glucose and xylose in synthetic media with the respective maximal specific rates of glucose and xylose consumption, and ethanol production of 3. Read More

View Article

Download full-text PDF

Source
http://link.springer.com/10.1007/s00449-019-02090-0
Publisher Site
http://dx.doi.org/10.1007/s00449-019-02090-0DOI Listing
February 2019
6 Reads

Recent developments in bioreactor scale production of bacterial polyhydroxyalkanoates.

Bioprocess Biosyst Eng 2019 Feb 27. Epub 2019 Feb 27.

School of Biomedical Sciences, University of Ulster, Coleraine, Northern Ireland, BT52 1SA, UK.

Polyhydroxyalkanoates (PHAs) are biological plastics that are sustainable alternative to synthetic ones. Numerous microorganisms have been identified as PHAs producers. They store PHAs as cellular inclusions to use as an energy source backup. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00449-019-02093-xDOI Listing
February 2019
1.997 Impact Factor

Application of a multiphase microreactor chemostat for the determination of reaction kinetics of Staphylococcus carnosus.

Bioprocess Biosyst Eng 2019 Feb 27. Epub 2019 Feb 27.

Institute of Biochemical Engineering, Technische Universität Braunschweig, Rebenring 56, 38106, Braunschweig, Germany.

Bioreactors at the microliter scale offer a promising approach to accelerate bioprocess development. Advantages of such microbioreactors include a reduction in the use of expensive reagents. In this study, a chemostat operation mode of a cuvette-based microbubble column bioreactor made of polystyrene (working volume of 550 µL) was demonstrated. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00449-019-02095-9DOI Listing
February 2019

On-line glucose monitoring by near infrared spectroscopy during the scale up steps of mammalian cell cultivation process development.

Bioprocess Biosyst Eng 2019 Feb 26. Epub 2019 Feb 26.

Department of Applied Biotechnology and Food Science, Budapest University of Technology and Economics, Műegyetem rkp. 3, Budapest, 1111, Hungary.

NIR spectroscopy is a non-destructive tool for in-situ, on-line bioprocess monitoring. One of its most frequent applications is the determination of metabolites during cultivation, especially glucose. Previous studies have usually investigated the applicability of Near Infrared (NIR) spectroscopy at one bioreactor scale but the effect of scale up was not explored. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00449-019-02091-zDOI Listing
February 2019

Model-assisted Design of Experiments as a concept for knowledge-based bioprocess development.

Bioprocess Biosyst Eng 2019 Feb 26. Epub 2019 Feb 26.

Hamburg University of Technology, Bioprocess and Biosystems Engineering, Denickestr. 15, 21073, Hamburg, Germany.

Design of Experiments methods offer systematic tools for bioprocess development in Quality by Design, but their major drawback is the user-defined choice of factor boundary values. This can lead to several iterative rounds of time-consuming and costly experiments. In this study, a model-assisted Design of Experiments concept is introduced for the knowledge-based reduction of boundary values. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00449-019-02089-7DOI Listing
February 2019

Improvement of kojic acid production in Aspergillus oryzae AR-47 mutant strain by combined mutagenesis.

Bioprocess Biosyst Eng 2019 Feb 25. Epub 2019 Feb 25.

School of Life Science and Technology, China Pharmaceutical University, Nanjing, 210009, China.

Kojic acid is a kind of secondary metabolites, whose biosynthesis pathway remains unclear to date. It is produced industrially by microbial fermentation, and thus, mutagenesis breeding still plays a vital role for obtaining strains with high kojic acid production. The starting strain KA-11 isolated from mildewed fruits was identified as Aspergillus oryzae and then subjected to a combined mutagenesis program including microwave mutagenesis, UV irradiation, heat-LiCl, atmospheric, and room temperature plasma (ARTP). Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00449-019-02079-9DOI Listing
February 2019
2 Reads

Butanol and butyric acid production from Saccharina japonica by Clostridium acetobutylicum and Clostridium tyrobutyricum with adaptive evolution.

Bioprocess Biosyst Eng 2019 Apr 20;42(4):583-592. Epub 2019 Feb 20.

Department of Biotechnology, Pukyong National University, Busan, 48513, South Korea.

Optimal conditions of hyper thermal (HT) acid hydrolysis of the Saccharina japonica was determined to a seaweed slurry content of 12% (w/v) and 144 mM HSO at 160 °C for 10 min. Enzymatic saccharification was carried out at 50 °C and 150 rpm for 48 h using the three enzymes at concentrations of 16 U/mL. Celluclast 1. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00449-018-02063-9DOI Listing
April 2019
8 Reads

Performance and microbial community profiles in pilot-scale biofilter for the simultaneous removal of ammonia, iron and manganese at different manganese concentrations.

Bioprocess Biosyst Eng 2019 Feb 19. Epub 2019 Feb 19.

State Key Laboratory of Urban Water Resource and Environment, School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin, 150090, People's Republic of China.

To accelerate extensive application of biological manganese removal technology, a pilot-scale biofilter for ammonia, iron and manganese removal was constructed to investigate the removal performance and microbial community profiles at different manganese concentrations. When manganese in influent increased from 1 to 10 mg/L, the pollutants were completely removed. Ammonia and iron was slightly changed along the filter depth, while manganese obviously increased. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00449-019-02077-xDOI Listing
February 2019

Intracellular response of Bacillus natto in response to different oxygen supply and its influence on menaquinone-7 biosynthesis.

Bioprocess Biosyst Eng 2019 Feb 13. Epub 2019 Feb 13.

College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, No. 30 South Puzhu Road, Nanjing, 211816, People's Republic of China.

Menaquinone-7 (MK-7) plays an important role in blood clotting, cardiovascular disease and anti-osteoporosis, and has been wildly used in the food additives and pharmaceutical industries. The aim of this study was to investigate the mechanism of menaquinone-7 biosynthesis in response to different oxygen supplies in Bacillus natto. The differences of fermentation performance, intracellular metabolites, oxidative stress reaction and enzyme activities of Bacillus natto R127 were analyzed under different KLa. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00449-019-02085-xDOI Listing
February 2019
2 Reads

Respirometry as a tool to quantify kinetic parameters of microalgal mixotrophic growth.

Bioprocess Biosyst Eng 2019 Feb 12. Epub 2019 Feb 12.

Department of Industrial Engineering DII, University of Padova, Via Marzolo 9, 35131, Padua, Italy.

Modeling microalgal mixotrophy is challenging, as the regulation of algal metabolism is affected by many environmental factors. A reliable tool to simulate microalgal behavior in complex systems, such as wastewaters, may help in setting the proper values of operative variables, provided that model parameters have been properly evaluated. In this work, a new respirometric protocol is proposed to quickly obtain the half-saturation constant values for several nutrients. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00449-019-02087-9DOI Listing
February 2019
1 Read

Recycling of cooking oil fume condensate for the production of rhamnolipids by Pseudomonas aeruginosa WB505.

Bioprocess Biosyst Eng 2019 Feb 11. Epub 2019 Feb 11.

Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education; School of Biotechnology, Jiangnan University, 1800 Lihu Av., Wuxi, 214122, Jiangsu, China.

Rhamnolipids (RLs) are anionic biosurfactants with great application potential. This study explored the possibility of producing RLs from cooking oil fume condensates (COFCs) collected from range hoods. A mutant of Pseudomonas aeruginosa AB93066 was obtained and used to produce RLs from COFCs as a substrate. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00449-019-02081-1DOI Listing
February 2019

Production of cutinase by solid-state fermentation and its use as adjuvant in bioherbicide formulation.

Bioprocess Biosyst Eng 2019 Feb 9. Epub 2019 Feb 9.

Department of Chemical and Food Engineering, Technology Center, Federal University of Santa Catarina, UFSC, Florianópolis, Brazil.

In the present study, it was presented a strategy to maximize the cutinase production by solid-state fermentation from different microorganisms and substrates. The best results were observed using Fusarium verticillioides, rice bran being the main substrate. Maximum yield of cutinase obtained by the strain was 16. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00449-019-02086-wDOI Listing
February 2019

Study of the mechanism for increased protein expression via transcription potency reduction of the selection marker.

Bioprocess Biosyst Eng 2019 Feb 7. Epub 2019 Feb 7.

Department of Cell Biol, Institute of Biomedicine & National Engineering Research Center of Genetic Medicine, College of Life Science and Technology, Jinan University, 730 Building of Biology, 601 W Huangpu Ave, Guangzhou, 510630, Guangdong, People's Republic of China.

Stable transfection of mammalian cells using various expression cassettes for exogenous gene expression has been well established. The impact of critical factors in these cassettes, such as promoter and enhancer elements, on recombinant protein production in mammalian cells has been studied extensively to optimize the expression efficiency. However, few studies on the correlation between the strength of selection marker and the expression of gene of interest (GOI) have been reported. Read More

View Article

Download full-text PDF

Source
http://link.springer.com/10.1007/s00449-019-02083-z
Publisher Site
http://dx.doi.org/10.1007/s00449-019-02083-zDOI Listing
February 2019
5 Reads

Automated image processing as an analytical tool in cell cryopreservation for bioprocess development.

Bioprocess Biosyst Eng 2019 Feb 4. Epub 2019 Feb 4.

Institute of Functional Interfaces (IFG), Karlsruhe Institute of Technology (KIT), Eggenstein-Leopoldshafen, Germany.

The continuous availability of cells with defined cell characteristics represents a crucial issue in the biopharmaceutical and cell therapy industry. Here, development of cell banks with a long-term stability is essential and ensured by a cryopreservation strategy. The strategy needs to be optimized for each cell application individually and usually comprises controlled freezing, storage at ultra-low temperature, and fast thawing of cells. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00449-019-02071-3DOI Listing
February 2019

Computer-aided design of microfluidic resistive network using circuit partition and CFD-based optimization and application in microalgae assessment for marine ecological toxicity.

Bioprocess Biosyst Eng 2019 Feb 4. Epub 2019 Feb 4.

Medical School, Dalian University, Dalian, 116622, China.

We present an automatic design process for microfluidic dilution network towards marine ecological toxicity assessment on microalgae. Based on the hydraulic-electric circuit analogy, we defined an abstract specification using computer-aided designing system. Several approaches, especially circuit partition, were applied to minimize design effort. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00449-019-02082-0DOI Listing
February 2019
12 Reads

Optimization of microbial cell disruption using pressurized CO for improving lipid recovery from wet biomass.

Bioprocess Biosyst Eng 2019 Feb 1. Epub 2019 Feb 1.

Dave C. Swalm School of Chemical Engineering, Mississippi State University, Mississippi State, MS, 39762, USA.

Microbial cell disruption using pressurized gases (e.g., CO) is a promising approach to improve the lipid recovery from wet oleaginous microorganisms by eliminating the energy-intensive drying required for conventional methods. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00449-019-02080-2DOI Listing
February 2019

Biosynthesis and accumulation of poly(3-hydroxybutyrate-co-3-hydroxyvalerate)-polyethylene glycol, a hybrid co-polymer by endophytic Bacillus cereus RCL 02.

Bioprocess Biosyst Eng 2019 Feb 1. Epub 2019 Feb 1.

Microbiology Laboratory, Department of Botany, University of Calcutta, 35, Ballygunge Circular Road, Kolkata, West Bengal, 700019, India.

Co-polymerization of microbial polyesters, polyhydroxyalkanoates (PHAs), with synthetic polymers has become an established and promising tool in the recent past for improving the material and biological properties of the biopolyesters. Bacillus cereus RCL 02, a leaf endophytic bacterium of the oleaginous plant Ricinus communis L., has been reported to produce a significant amount of poly(3-hydroxybutyrate) [P(3HB)] and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) [P(3HB-co-3HV)] under batch cultivation. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00449-019-02084-yDOI Listing
February 2019

Anodic microbial community analysis of microbial fuel cells based on enriched inoculum from freshwater sediment.

Bioprocess Biosyst Eng 2019 Jan 29. Epub 2019 Jan 29.

Department of Public Health and Pediatrics, University of Torino, Piazza Polonia 94, 10126, Turin, Italy.

The characterization of anodic microbial communities is of great importance in the study of microbial fuel cells (MFCs). These kinds of devices mainly require a high abundance of anode respiring bacteria (ARB) in the anode chamber for optimal performance. This study evaluated the effect of different enrichments of environmental freshwater sediment samples used as inocula on microbial community structures in MFCs. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00449-019-02074-0DOI Listing
January 2019

Rational high-throughput system for screening of high sophorolipids-producing strains of Candida bombicola.

Bioprocess Biosyst Eng 2019 Apr 24;42(4):575-582. Epub 2019 Jan 24.

State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, P.O. Box 329#, Shanghai, 200237, People's Republic of China.

A rational high-throughput screening can significantly improve the efficiency of strain screening with high performance. In this study, based on the addition reaction of unsaturated fatty acids in the sophorolipids (SLs) and I molecules, a simple and rapid high-throughput detection method for SLs was established which demonstrated a correlation coefficient (R) of 0.9106 with high-performance liquid chromatography (HPLC) method. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00449-018-02062-wDOI Listing
April 2019
1 Read