Biopolymers 2017 Nov 15. Epub 2017 Nov 15.
Department of Chemistry, Indian Institute of Technology Kharagpur, Kharagpur, 721302, India.
Formation of dityrosine (DT) cross-linkages in proteins is one of the most widely used markers of oxidative stress. Ribonuclease A (RNase A) has 6 Tyr residues and shows a characteristic DT fluorescence peak upon oxidation in addition to major changes in its secondary structure. DT formation can be prevented by using polyphenols (GA, ECG, and EGCG) which are known to have strong antioxidant activity. Read More