Biopolymers 2018 May 6:e23222. Epub 2018 May 6.
College of Biological Science and Engineering, Fuzhou University, Fuzhou, 350002, China.
A recoverable and thermoresponsive polymer-protein bioconjugate is synthesized and employed in the purification of protein with free sulfhydryl groups. Initiator with disulphide was modified on the cysteine residue of the target protein. Poly(N-isopropylacrylamide) exhibiting a lower critical solution temperature was grown from the protein. Read More