Impact of bistrand abasic sites and proximate orientation on DNA global structure and duplex energetics.
- Conceição A Minetti,
- Jeffrey Y Sun,
- Daniel P Jacobs,
- Inkoo Kang,
- David P Remeta,
- Kenneth J Breslauer
Biopolymers 2018 Jan 11. Epub 2018 Jan 11.
Department of Chemistry and Chemical Biology, Rutgers - The State University of New Jersey, Piscataway, New Jersey.
Bistrand lesions embedded within a single helical turn of tridecameric deoxyoligonucleotide duplexes represent a model system for exploring the impact of clustered lesions that occur in vivo and pose a significant challenge to cellular repair machineries. Such investigations are essential for understanding the forces that dictate lesion-induced mutagenesis, carcinogenesis, and cytotoxicity within a context that mimics local helical perturbations caused by an ionizing radiation event. This study characterizes the structural and energy profiles of DNA duplexes harboring synthetic abasic sites (tetrahydrofuran, F) as models of clustered bistrand abasic (AP) lesions. Read More