15,166 results match your criteria Bioorganic & medicinal chemistry letters[Journal]


Acute liver failure with severe steatosis induced by mushroom.

Liver Int 2020 Jul 8. Epub 2020 Jul 8.

Department of Metabolic Regulation, Shinshu University School of Medicine, Matsumoto, Japan.

A 26-year-old man developed nausea and vomiting 4 hours after eating white mushrooms. (Figure 1a) When he visited his primary physician the next day, liver function tests were unremarkable. Since his symptoms did not improve, he was admitted to our hospital on the fourth day after mushroom intake. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/liv.14588DOI Listing

Invasive liver abscess syndrome accompanied by spondylodiscitis: a case report and review of the literature.

Clin J Gastroenterol 2020 Jul 7. Epub 2020 Jul 7.

Department of Internal Medicine, Nagano Prefectural Kiso Hospital, Kiso, Japan.

A 65-year-old man who had diabetes mellitus was referred to our hospital due to fever and back pain. Laboratory findings showed liver dysfunction and C-reactive protein (CRP) elevation. Enhanced computed tomography (CT) showed multiple liver abscesses, hepatic vein and inferior vena cava thrombosis, and spondylodiscitis in the fourth lumbar vertebrae. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s12328-020-01161-0DOI Listing

Enhancement of protoplast preparation and regeneration of Hirsutella sinensis based on process optimization.

Biotechnol Lett 2020 Jul 8. Epub 2020 Jul 8.

Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, 310014, People's Republic of China.

Objective: To explore the optimal methods for the protoplast preparation and regeneration of Hirsutella sinensis by optimizing the limiting factors.

Results: During the treatment of enzymatic protoplast preparation, mycelium cultured for 7 days was the optimal start material. The maximum protoplast preparation rate of 4. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s10529-020-02958-2DOI Listing

A kinase bioscavenger provides antibiotic resistance by extremely tight substrate binding.

Sci Adv 2020 Jun 24;6(26):eaaz9861. Epub 2020 Jun 24.

Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, Moscow, Russia.

Microbial communities are self-controlled by repertoires of lethal agents, the antibiotics. In their turn, these antibiotics are regulated by bioscavengers that are selected in the course of evolution. Kinase-mediated phosphorylation represents one of the general strategies for the emergence of antibiotic resistance. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1126/sciadv.aaz9861DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7314540PMC

Data on nucleoid-associated proteins isolated from in different growth phases.

Data Brief 2020 Aug 12;31:105853. Epub 2020 Jun 12.

Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, Moscow, Russia.

(MG) is one of the smallest free-living and self-replicating organisms, it is characterized by lack of cell wall and reduced genome size. As a result of genome reduction, MG has a limited variety of DNA-binding proteins and transcription factors. To investigate the dynamic changes of the proteomic profile of MG nucleoid, that may assist in revealing its mechanisms of functioning, regulation of chromosome organization and stress adaptation, a quantitative proteomic study was performed on MG nucleoids obtained from the cell culture in logarithmic and stationary phases of synchronous growth. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.dib.2020.105853DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7327420PMC

Direct Delivery of Health Promoting β-Asp-Arg Dipeptides via Stable Co-expression of Cyanophycin and the Cyanophycinase CphE241 in Tobacco Plants.

Front Plant Sci 2020 19;11:842. Epub 2020 Jun 19.

Department of Agrobiotechnology and Risk Assessment for Bio- und Gene Technology, Faculty of Agricultural and Environmental Sciences, University of Rostock, Rostock, Germany.

Feed supplementation with β-arginine-aspartate dipeptides (β-Asp-Arg DP) shows growth promoting effects in feeding trials with fish and might also be beneficial for pig and poultry farming. Currently, these DPs are generated from purified cyanophycin (CGP), with the help of the CGP-degrading enzyme cyanophycinase (CGPase). As alternative to an production, the DPs might be directly produced in feed crops. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3389/fpls.2020.00842DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7318851PMC

Inhibition of interaction between Staphylococcus aureus α-hemolysin and erythrocytes membrane by hydrolysable tannins: structure-related activity study.

Sci Rep 2020 Jul 7;10(1):11168. Epub 2020 Jul 7.

Department of Microbiology and Biotechnology, Laboratory of Molecular Biophysics, Faculty of Biology, University of Bialystok, Konstanty Ciolkowski Street 1J, 15-245, Białystok, Poland.

The objective of the study was a comparative analysis of the antihemolytic activity against two Staphylococcus aureus strains (8325-4 and NCTC 5655) as well as α-hemolysin and of the membrane modifying action of four hydrolysable tannins with different molecular mass and flexibility: 3,6-bis-O-di-O-galloyl-1,2,4-tri-O-galloyl-β-D-glucose (T1), 1,2,3,4,5-penta-O-galloyl-β-D-glucose (T2), 3-O-galloyl-1,2-valoneoyl-β-D-glucose (T3) and 1,2-di-O-galloyl-4,6-valoneoyl-β-D-glucose (T4). We showed that all the compounds studied manifested antihemolytic effects in the range of 5-50 µM concentrations. However, the degree of the reduction of hemolysis by the investigated tannins was not uniform. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41598-020-68030-1DOI Listing

Autoantibodies from SLE patients induce programmed cell death in murine fibroblast cells through interaction with TNFR1 receptor.

Sci Rep 2020 Jul 7;10(1):11144. Epub 2020 Jul 7.

Laboratory of Molecular Immunogenetics of Cancer, Institute of Gene Biology RAS, Moscow, Russia, 119334.

Various pathological processes are known to be associated with the production of IgG autoantibodies, which have high affinity for self-antigens and often cause tissue injury and the development of autoimmune diseases. However, the mechanism of their cytotoxic activity is not clearly understood yet. Here, we have shown that the action of these autoantibodies on cells expressing TNFR1 (the cell surface receptor for TNFα) can cause both caspase-dependent apoptosis and necroptosis of these cells, with suppression of apoptosis resulting in switching to RIP1-dependent necroptosis. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41598-020-68088-xDOI Listing

Synthesis and Characterization of Low-Cost Cresol-Based Benzoxazine Resins as Potential Binders in Abrasive Composites.

Materials (Basel) 2020 Jul 5;13(13). Epub 2020 Jul 5.

Institute of Chemical Technology and Engineering, Faculty of Chemical Technology, Poznan University of Technology, Berdychowo 4, PL-60965 Poznan, Poland.

A series of cresol-based benzoxazines were synthesized for potential application as a polymer matrix in abrasive composites. The chemical structures of the obtained benzoxazine resins were investigated in detail using Fourier transform infrared spectroscopy (FTIR) and hydrogen-1 as well as carbon-13 nuclear magnetic resonance spectroscopy (H NMR, C NMR) with an additional analysis using two-dimensional NMR techniques (2D NMR H-H COSY, H-C gHSQC and gHMBC). Structural analysis confirmed the presence of vibrations of -O-C-N- at ~950 cm wavenumber, characteristic for an oxazine ring. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3390/ma13132995DOI Listing

Marine Excitatory Amino Acids: Structure, Properties, Biosynthesis and Recent Approaches to Their Syntheses.

Molecules 2020 Jul 3;25(13). Epub 2020 Jul 3.

National Scientific Center of Marine Biology, Far Eastern Branch, Russian Academy of Sciences, ul. Palchevskogo, 17, 690041 Vladivostok, Russia.

This review considers the results of recent studies on marine excitatory amino acids, including kainic acid, domoic acid, dysiherbaine, and neodysiherbaine A, known as potent agonists of one of subtypes of glutamate receptors, the so-called kainate receptors. Novel information, particularly concerning biosynthesis, environmental roles, biological action, and syntheses of these marine metabolites, obtained mainly in last 10-15 years, is summarized. The goal of the review was not only to discuss recently obtained data, but also to provide a brief introduction to the field of marine excitatory amino acid research. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3390/molecules25133049DOI Listing

Phage PaBG-A Jumbo Member of an Old Parasite Family.

Viruses 2020 Jul 3;12(7). Epub 2020 Jul 3.

Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997 Moscow, Russia.

Bacteriophage PaBG is a jumbo Myoviridae phage isolated from water of Lake Baikal. This phage has limited diffusion ability and thermal stability and infects a narrow range of strains. Therefore, it is hardly suitable for phage therapy applications. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3390/v12070721DOI Listing

SpCas9- and LbCas12a-Mediated DNA Editing Produce Different Gene Knockout Outcomes in Zebrafish Embryos.

Genes (Basel) 2020 Jul 3;11(7). Epub 2020 Jul 3.

Department of Biochemistry, St. Petersburg State University, 199178 St. Petersburg, Russia.

CRISPR/Cas (Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR associated protein) genome editing is a powerful technology widely used in current genetic research. In the most simple and straightforward way it can be applied for a gene knockout resulting from repair errors, induced by dsDNA cleavage by Cas nuclease. For decades, zebrafish () has been known as a convenient model object of developmental biology. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3390/genes11070740DOI Listing

The insecticidal wasp toxin Γ-Pompilidotoxin is a linear peptide modulating voltage-gated sodium channels.

Toxicon 2020 Apr;177 Suppl 1:S40

Toxicology and Pharmacology, Katholieke Universiteit (KU) Leuven, Campus Gasthuisberg, Leuven, Belgium; M.M. Shemyakin & Yu.A. Ovchinnikov Institute of Bioorganic Chemistry, The Russian Academy of Sciences, Russia; Institute for Molecular Bioscience, The University of Queensland, Brisbane, Queensland, Australia; Madrid Institute for Advanced Studies (IMDEA) in Food, CEI UAM+CSIC, Madrid, E28049, Spain; Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Higashi, Tsukuba, Ibaraki, 305-8566, Japan; Institute of Natural Medicine, University of Toyama, Toyama, Toyama, 930-0194, Japan; M.M. Shemyakin & Yu.A. Ovchinnikov Institute of Bioorganic Chemistry, The Russian Academy of Sciences, Mikhluho-Maklaya str. 16/10, Moscow, 117997, Russia.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.toxicon.2019.12.069DOI Listing

P2X3 receptor antagonists from spider venom.

Toxicon 2020 Apr;177 Suppl 1:S3

Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, Russia; Future Analgesics Ltd, Moscow, Russia.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.toxicon.2019.10.017DOI Listing

Sea anemone peptide modulates TRPA1 activity, produces analgesia and enhances process of regeneration.

Toxicon 2020 Apr;177 Suppl 1:S21-S22

Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia; Sechenov First Moscow State Medical University, Institute of Molecular Medicine, Moscow, Russia.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.toxicon.2019.10.249DOI Listing

Channel blockers from scorpion venoms inhibit nicotinic acetylcholine receptors.

Toxicon 2020 Apr;177 Suppl 1:S11

Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia; Sechenov First Moscow State Medical University, Institute of Molecular Medicine, Moscow, Russia.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.toxicon.2019.10.049DOI Listing

Three finger neurotoxins: Recent discoveries and arising questions.

Toxicon 2020 Apr;177 Suppl 1:S10-S11

Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, Moscow, 117997, Russia; Institute of Occupational Medicine, Social Medicine and Environmental Medicine, Goethe University, Frankfurt am Main, Germany; Institute of Legal Medicine, Goethe University, Frankfurt am Main, Germany.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.toxicon.2019.10.048DOI Listing

Epitope Analysis of Murine and Chimeric Monoclonal Antibodies Recognizing the Cancer Testis Antigen PRAME.

Dokl Biochem Biophys 2020 May 6;492(1):135-138. Epub 2020 Jul 6.

Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.

We investigated the epitope specificity of different monoclonal antibodies recognizing the cancer testis antigen PRAME. Antibody 5D3 binds to the fragment of PRAME corresponding to 160-180 amino acid residues. Antibodies 6H8 and F11 bind to the fragment corresponding to 180-200 amino acid residues of PRAME. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1134/S1607672920030072DOI Listing

DiB-splits: nature-guided design of a novel fluorescent labeling split system.

Sci Rep 2020 Jul 6;10(1):11049. Epub 2020 Jul 6.

Department of Chemistry, Center for Structural Biology, Vanderbilt University, Nashville, TN, 37235, USA.

Fluorogen-activating proteins (FAPs) are innovative fluorescent probes combining advantages of genetically-encoded proteins such as green fluorescent protein and externally added fluorogens that allow for highly tunable and on demand fluorescent signaling. Previously, a panel of green- and red-emitting FAPs has been created from bacterial lipocalin Blc (named DiBs). Here we present a rational design as well as functional and structural characterization of the first self-assembling FAP split system, DiB-splits. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41598-020-67095-2DOI Listing

Enhancement of the blood-circulation time and performance of nanomedicines via the forced clearance of erythrocytes.

Nat Biomed Eng 2020 Jul 6. Epub 2020 Jul 6.

Prokhorov General Physics Institute of the Russian Academy of Sciences, Moscow, Russia.

The rapid elimination of nanoparticles from the bloodstream by the mononuclear phagocyte system limits the activity of many nanoparticle formulations. Here, we show that inducing a slight and transient depletion of erythrocytes in mice (~5% decrease in haematocrit) by administrating a low dose (1.25 mg kg) of allogeneic anti-erythrocyte antibodies increases the circulation half-life of a range of short-circulating and long-circulating nanoparticle formulations by up to 32-fold. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41551-020-0581-2DOI Listing

Evolution of abiotic cubane chemistries in a nucleic acid aptamer allows selective recognition of a malaria biomarker.

Proc Natl Acad Sci U S A 2020 Jul 6. Epub 2020 Jul 6.

School of Biomedical Sciences, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China;

Nucleic acid aptamers selected through systematic evolution of ligands by exponential enrichment (SELEX) fold into exquisite globular structures in complex with protein targets with diverse translational applications. Varying the chemistry of nucleotides allows evolution of nonnatural nucleic acids, but the extent to which exotic chemistries can be integrated into a SELEX selection to evolve nonnatural macromolecular binding interfaces is unclear. Here, we report the identification of a cubane-modified aptamer (cubamer) against the malaria biomarker lactate dehydrogenase (PvLDH). Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1073/pnas.2003267117DOI Listing

Anticonvulsant and analgesic in neuropathic pain activity in a group of new aminoalkanol derivatives.

Bioorg Med Chem Lett 2020 Aug 7;30(16):127325. Epub 2020 Jun 7.

Jagiellonian University Medical College, Faculty of Pharmacy, Chair of Organic Chemistry, Department of Bioorganic Chemistry, Medyczna 9, 30-688 Kraków, Poland. Electronic address:

As part of the presented research, thirteen new aminoalkanol derivatives were designed and obtained by chemical synthesis. In vivo studies (mice, i.p. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bmcl.2020.127325DOI Listing

Therapeutic Editing of the Gene: Is CRISPR/Cas9 an Option?

Genes (Basel) 2020 Jun 25;11(6). Epub 2020 Jun 25.

Kazan Federal University, 420008 Kazan, Russia.

The gene encodes the transcription factor and oncosuppressor p53 protein that regulates a multitude of intracellular metabolic pathways involved in DNA damage repair, cell cycle arrest, apoptosis, and senescence. In many cases, alterations (e.g. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3390/genes11060704DOI Listing

Metabolomic Signature Discriminates Normal Human Cornea from Keratoconus-A Pilot GC/MS Study.

Molecules 2020 Jun 25;25(12). Epub 2020 Jun 25.

Department of Ophthalmology, District Railway Hospital, Panewnicka 65, 40-760 Katowice, Poland.

The molecular etiology of keratoconus (KC), a pathological condition of the human cornea, remains unclear. The aim of this work was to perform profiling of metabolites and identification of features discriminating this pathology from the normal cornea. The combination of gas chromatography and mass spectrometry (GC/MS) techniques has been applied for profiling and identification of metabolites in corneal buttons from 6 healthy controls and 7 KC patients. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3390/molecules25122933DOI Listing

Antifungal Agent 4-AN Changes the Genome-Wide Expression Profile, Downregulates Virulence-Associated Genes and Induces Necrosis in Cells.

Molecules 2020 Jun 25;25(12). Epub 2020 Jun 25.

Department of Molecular Biology, Institute of Biological Sciences, The John Paul II Catholic University of Lublin, ul. Konstantynów 1i, 20-708 Lublin, Poland.

In the light of the increasing occurrence of antifungal resistance, there is an urgent need to search for new therapeutic strategies to overcome this phenomenon. One of the applied approaches is the synthesis of small-molecule compounds showing antifungal properties. Here we present a continuation of the research on the recently discovered anti- agent 4-AN. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3390/molecules25122928DOI Listing

Deciphering the Binding of Salicylic Acid to Chloroplastic GAPDH-A1.

Int J Mol Sci 2020 Jun 30;21(13). Epub 2020 Jun 30.

IEES-Paris (UMR_7618) - Institut D'écologie et des Sciences de L'environnement de Paris, CNRS UMR 7583, Université Paris-Est Créteil, Sorbonne Université, F-94010 Paris, France.

Salicylic acid (SA) has an essential role in the responses of plants to pathogens. SA initiates defence signalling via binding to proteins. NPR1 is a transcriptional -activator and a key target of SA binding. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3390/ijms21134678DOI Listing

Cancer Stem Cell-Inducing Media Activates Senescence Reprogramming in Fibroblasts.

Cancers (Basel) 2020 Jun 30;12(7). Epub 2020 Jun 30.

Institute of Bioorganic Chemistry of the Polish Academy of Sciences, Zygmunta Noskowskiego 12/14, 61-704 Poznań, Poland.

Cellular senescence is a tumor-suppressive mechanism blocking cell proliferation in response to stress. However, recent evidence suggests that senescent tumor cells can re-enter the cell cycle to become cancer stem cells, leading to relapse after cancer chemotherapy treatment. Understanding how the senescence reprogramming process is a precursor to cancer stem cell formation is of great medical importance. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3390/cancers12071745DOI Listing

Enhancing the Effect of Pharmacological Ascorbate in Cancer Therapy via Acid-Triggered Ferritin Nanoparticles.

Adv Biosyst 2019 May 25;3(5):e1900006. Epub 2019 Mar 25.

Synthetic and Functional Biomolecules Center, Beijing National Laboratory for Molecular Sciences, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, College of Chemistry and Molecular Engineering, Innovation Center for Genomics, Peking University, Beijing, 100871, China.

The application of ascorbate (vitamin C) for cancer therapy was first proposed in the 1970s and has shown promising results in recent clinical trials. Pharmacological doses of ascorbate selectively induce cell death in different types of cancer cells through the generation of H O However, some cancer cells are resistant to ascorbate. So increasing the sensitivity of resistant cancer cells to ascorbate has attracted considerable attention. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/adbi.201900006DOI Listing

The effect of introducing an ether group into an imidazolium-based ionic liquid in binary mixtures with DMSO.

Phys Chem Chem Phys 2020 Jul 6. Epub 2020 Jul 6.

Engineering Thermodynamics, University of Bremen, Bremen, 28359, Germany.

Ether-functionalized ionic liquids (ILs) have successfully been employed in diverse applications, but their interactions with other solvents are not understood well. In this work, mixtures of 1-methoxyethyl-3-methylimidazolium bis(fluorosulfonyl)imide (EOMIMFSI) and dimethylsulfoxide (DMSO) are studied in terms of their solution structure and hydrogen bonding interactions. The corresponding alkyl-substituted IL 1-ethyl-3-methylimidazolium bis(fluorosulfonyl)imide (EMIMFSI) is analyzed for comparison. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1039/d0cp01568gDOI Listing

Structure of apo flavin-dependent halogenase Xcc4156 hints at a reason for cofactor-soaking difficulties.

Acta Crystallogr D Struct Biol 2020 Jul 30;76(Pt 7):687-697. Epub 2020 Jun 30.

Structural Biochemistry (BCIV), Department of Chemistry, Bielefeld University, Universitaetsstrasse 25, 33615 Bielefeld, Germany.

Flavin-dependent halogenases regioselectively introduce halide substituents into electron-rich substrates under mild reaction conditions. For the enzyme Xcc4156 from Xanthomonas campestris, the structure of a complex with the cofactor flavin adenine dinucleotide (FAD) and a bromide ion would be of particular interest as this enzyme exclusively brominates model substrates in vitro. Apo Xcc4156 crystals diffracted to 1. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1107/S2059798320007731DOI Listing

A new modulated crystal structure of the ANS complex of the St John's wort Hyp-1 protein with 36 protein molecules in the asymmetric unit of the supercell.

Acta Crystallogr D Struct Biol 2020 Jul 17;76(Pt 7):653-667. Epub 2020 Jun 17.

Center for Biocrystallographic Research, Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznan, Poland.

Superstructure modulation, with violation of the strict short-range periodic order of consecutive crystal unit cells, is well known in small-molecule crystallography but is rarely reported for macromolecular crystals. To date, one modulated macromolecular crystal structure has been successfully determined and refined for a pathogenesis-related class 10 protein from Hypericum perforatum (Hyp-1) crystallized as a complex with 8-anilinonaphthalene-1-sulfonate (ANS) [Sliwiak et al. (2015), Acta Cryst. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1107/S2059798320006841DOI Listing

Development and applications of deep eutectic solvents derived functional materials in chromatographic separation.

J Sep Sci 2020 Jul 6. Epub 2020 Jul 6.

Beijing National Laboratory for Molecular Sciences, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, Institute of Analytical Chemistry, College of Chemistry and Molecular Engineering, Peking University, Beijing, 100871, China.

Deep eutectic solvents have received increasing attentions over the past decade, and the green and versatile nature makes them important media for developing environmentally friendly and sustainable technologies. In addition to act as green alternatives to traditional organic solvents, deep eutectic solvents have proved to offer tremendous opportunities to produce different kinds of emerging functional materials. The present review highlights the recent development and applications of deep eutectic solvents-based functional materials as novel adsorbents for diverse analytes in complex matrices. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/jssc.202000523DOI Listing

The conformational analyses of 2-amino-N-[2-(dimethylphenoxy)ethyl]propan-1-ol derivatives in different environments.

Acta Crystallogr C Struct Chem 2020 07 27;76(Pt 7):681-689. Epub 2020 Jun 27.

Pedagogical University, Institute of Biology, Podchorążych 2, 30-084 Kraków, Poland.

Four crystal structures of 2-amino-N-(dimethylphenoxyethyl)propan-1-ol derivatives, characterized by X-ray diffraction analysis, are reported. The free base (R,S)-2-amino-N-[2-(2,3-dimethylphenoxy)ethyl]propan-1-ol, CHNO, 1, crystallizes in the space group P2/n, with two independent molecules in the asymmetric unit. The hydrochloride, (S)-N-[2-(2,6-dimethylphenoxy)ethyl]-1-hydroxypropan-2-aminium chloride, CHNO·Cl, 2c, crystallizes in the space group P2, with one cation and one chloride anion in the asymmetric unit. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1107/S2053229620008244DOI Listing

Development of fluorescence oligonucleotide probes based on cytosine- and guanine-rich sequences.

Sci Rep 2020 Jul 3;10(1):11006. Epub 2020 Jul 3.

Faculty of Chemistry, Adam Mickiewicz University, Uniwersytetu Poznanskiego 8, 61-614, Poznan, Poland.

The properties of cytosine- and guanine-rich oligonucleotides contributed to employing them as sensing elements in various biosensors. In this paper, we report our current development of fluorescence oligonucleotide probes based on i-motif or G-quadruplex forming oligonucleotides for cellular measurements or bioimaging applications. Additionally, we also focus on the spectral properties of the new fluorescent silver nanoclusters based system (ChONC12-AgNCs) that is able to anchor at the Langmuir monolayer interface, which is mimicking the surface of living cells membrane. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41598-020-67745-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7335195PMC

Retraction notice to "Direct and simultaneous detection of organic and inorganic ingredients in herbal powder preparations by Fourier transform infrared microspectroscopic imaging" [SAA (2016) 176-182].

Spectrochim Acta A Mol Biomol Spectrosc 2020 Jun 30:118605. Epub 2020 Jun 30.

Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology (Ministry of Education), Department of Chemistry, Tsinghua University, Beijing 100084, China.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.saa.2020.118605DOI Listing

Synthesis, antibacterial and antifungal activity of new 3-biphenyl-3H-Imidazo[1,2-a]azepin-1-ium bromides.

Eur J Med Chem 2020 Jun 6;201:112477. Epub 2020 Jun 6.

Institute of Pharmacology and Toxicology, Antona Tsedika 14, 03057, Kyiv, Ukraine; Nizhyn Mykola Gogol State University, Grafska 2, Nizhyn, Ukraine.

Novel 1-aryl-3-biphenyl-4-yl-3-hydroxy-2,5,6,7,8,9-hexahydro-3H-imidazo[1,2-a]azepin-1-ium bromides and their 2,5-dehydrogenated analogues were designed and synthesized using a reaction of aryl-(4,5,6,7-tetrahydro-3H-azepin-2-yl)-amines with 1-biphenyl-4-yl-2-bromoethanone. Among the 16 novel compounds 5 derivatives displayed in vitro antimicrobial activity; while three of them showed promising activity against Staphylococcus aureus, Cryptococcus neoformans and Candida albicans. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ejmech.2020.112477DOI Listing
June 2020
3.447 Impact Factor

Engineering Caspase 7 as an Affinity Reagent to Capture Proteolytic Products.

FEBS J 2020 Jul 3. Epub 2020 Jul 3.

Graduate School of Biomedical Sciences, Sanford Burnham Prebys Medical Discovery Institute, La Jolla, CA, 92037, United States.

Many proteases recognize their substrates with high specificities, with this in mind, it should theoretically be possible to utilize the substrate binding cleft of a protease as a scaffold to engineer an affinity reagent. In this study, we sought to develop reagents that would differentiate between substrates and products of proteolysis, based on a caspase 7 scaffold. Firstly, we engineered a form of caspase 7 that can undergo conversion to a substrate binding conformation without catalysis. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/febs.15467DOI Listing

Structural characteristics and anti-complement activities of polysaccharides from Sargassum hemiphyllum.

Glycoconj J 2020 Jul 2. Epub 2020 Jul 2.

College of Biotechnology and Bioengineering, Zhejiang University of Technology, 310014, Hangzhou, China.

Three polysaccharides (SH-1, SH-2 and SH-3) were purified from a brown macroalgea, Sargassum hemiphyllum. The autohydrolysis products from each polysaccharide were separated to three fractions (S fractions as oligomers, L fractions as low molecular weight polysaccharides and H fractions as high molecular weight polysaccharides). Mass spectroscopy of S fractions (SH-1-S, SH-2-S and SH-3-S) showed that these three polymers all contained short stretches of sulfated fucose. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s10719-020-09928-wDOI Listing

Nuclear immunophilin FKBP39 from Drosophila melanogaster drives spontaneous liquid-liquid phase separation.

Int J Biol Macromol 2020 Jun 30;163:108-119. Epub 2020 Jun 30.

Department of Biochemistry, Molecular Biology and Biotechnology, Faculty of Chemistry, Wroclaw University of Science and Technology, Wybrzeże Wyspiańskiego 27, 50-370 Wroclaw, Poland.

The FKBP39 from Drosophila melanogaster is a multifunctional regulatory immunophilin. It contains two globular domains linked by a highly charged disordered region. The N-terminal domain shows homology to the nucleoplasmin core domain, and the C-terminal domain is characteristic for the family of the FKBP immunophilin ligand binding domain. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ijbiomac.2020.06.255DOI Listing

Dimerization of conserved ascaroside building blocks generates species-specific male attractants in Caenorhabditis nematodes.

Org Biomol Chem 2020 Jul 2. Epub 2020 Jul 2.

Department of Bioorganic Chemistry, Max Planck Institute for Chemical Ecology, Hans-Knöll Straße 8, D-07745 Jena, Germany and Laboratory for Bioanalytical Chemistry, Institute of Chemistry, University of Neuchâtel, Avenue de Bellevaux 51, CH-2000 Neuchâtel, Switzerland.

Comparative ascaroside profiling of Caenorhabditis nematodes using HPLC-ESI-(-)-MS/MS precursor ion scanning revealed a class of highly species-specific ascaroside dimers. Their 2- and 4-isomeric, homo- and heterodimeric structures were identified using a combination of HPLC-ESI-(+)-HR-MS/MS spectrometry and high-resolution dqf-COSY NMR spectroscopy. Structure assignments were confirmed by total synthesis of representative examples. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1039/d0ob00799dDOI Listing

Professor Dr. Geoffrey Burnstock (1929-2020).

Purinergic Signal 2020 Jul 2. Epub 2020 Jul 2.

Institute of Cell Biology and Neuroscience, Molecular and Cellular Neurobiology, Goethe University, Max-von-Laue-Str. 13, 60438, Frankfurt am Main, Germany.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s11302-020-09709-yDOI Listing

Cystathionine β-synthase deficiency: different changes in proteomes of thrombosis-resistant Cbs mice and thrombosis-prone CBS humans.

Sci Rep 2020 Jul 1;10(1):10726. Epub 2020 Jul 1.

Department of Biochemistry and Biotechnology, University of Life Sciences, 60-632, Poznan, Poland.

Cystathionine β-synthase (CBS)-deficient patients are prone to vascular thrombosis. In contrast, Cbs mice show no abnormalities in blood coagulation. To identify molecular basis underlying these disparately different thrombotic phenotypes, we analyzed plasma proteomes of Cbs vs. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41598-020-67672-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7329814PMC

Immunosuppressive properties of cytochalasin B-induced membrane vesicles of mesenchymal stem cells: comparing with extracellular vesicles derived from mesenchymal stem cells.

Sci Rep 2020 Jul 1;10(1):10740. Epub 2020 Jul 1.

Institute of Fundamental Medicine and Biology, Kazan (Volga Region) Federal University, Kazan, Russia, 420008.

Extracellular vesicles derived from mesenchymal stem cells (MSCs) represent a novel approach for regenerative and immunosuppressive therapy. Recently, cytochalasin B-induced microvesicles (CIMVs) were shown to be effective drug delivery mediators. However, little is known about their immunological properties. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41598-020-67563-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7330035PMC

Intra-articular Injection of Cell-laden 3D Microcryogels Empower Low-dose Cell Therapy for Osteoarthritis in a Rat Model.

Cell Transplant 2020 Jan-Dec;29:963689720932142

Arthritis Clinic & Research Center, Peking University People's Hospital, Peking University, Beijing, China.

Intra-articular injection of mesenchymal stem cells (MSCs) in an osteoarthritic joint can help slow down cartilage destruction. However, cell survival and the efficiency of repair are generally low due to mechanical damage during injection and a high rate of cell loss. We, thus, investigated an improved strategy for cell delivery to an osteoarthritic joint through the use of three-dimensional (3D) microcryogels. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1177/0963689720932142DOI Listing

An intelligent film actuator with multi-level deformation behaviour.

Nanoscale Horiz 2020 Jul 1. Epub 2020 Jul 1.

Key Laboratory for Advanced Materials Processing Technology, Ministry of Education of China, State Key Laboratory of Tribology, Department of Mechanical Engineering, Tsinghua University, Beijing 100084, China. and Department of Chemistry, MOE Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology, Tsinghua University, Beijing 100084, China.

Smart materials with simply reversible deformation or reconfigurability have shown great potential in artificial muscles, robots, controlled displays, etc. However, the combination of reversible and reconfigured functions in responsive materials, which can endow them with mature and complex actuation performance similar to that of living things, is still a great challenge. In this regard, we report an intelligent graphene oxide (GO)/polyvinylidene fluoride (PVDF) film with reconfigured structures resulting from inner plastic deformation after treatment at elevated temperature (40-80 °C), which simultaneously expresses basically and secondarily reversible deformation ability of its original and reconfigured states in response to external stimuli (e. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1039/d0nh00268bDOI Listing

Integrated proteomic and metabolomic analyses revealed molecular adjustments in Populus × canescens colonized with the ectomycorrhizal fungus Paxillus involutus, which limited plant host growth.

Environ Microbiol 2020 Jun 30. Epub 2020 Jun 30.

Polish Academy of Sciences, Institute of Dendrology, Parkowa 5, PL-62035, Kórnik, Poland.

Ectomycorrhizae (ECMs) are a highly context-dependent interactions that are not always beneficial for the plant host, sometimes leading to a decrease in plant growth. However, the molecular status of these plants remains unknown. We studied Populus × canescens microcuttings characterized by impaired growth in response to colonization by a Paxillus involutus strain via integrative proteomics-metabolomics analyses. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/1462-2920.15146DOI Listing

Chrysomycin A Derivatives for the Treatment of Multi-Drug-Resistant Tuberculosis.

ACS Cent Sci 2020 Jun 4;6(6):928-938. Epub 2020 May 4.

Beijing National Laboratory for Molecular Sciences, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, Department of Chemical Biology, College of Chemistry and Molecular Engineering, Synthetic and Functional Biomolecules Center, and Peking-Tsinghua Center for Life Sciences, Peking University, Beijing 100871, China.

Tuberculosis (TB) is a life-threatening disease resulting in an estimated 10 million new infections and 1.8 million deaths annually, primarily in underdeveloped countries. The economic burden of TB has been estimated as approximately 12 billion USD annually in direct and indirect costs. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1021/acscentsci.0c00122DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7318084PMC

h-FIBER: Microfluidic Topographical Hollow Fiber for Studies of Glomerular Filtration Barrier.

ACS Cent Sci 2020 Jun 13;6(6):903-912. Epub 2020 May 13.

Institute for Biomaterials and Biomedical Engineering, University of Toronto, 164 College Street, Toronto, Ontario M5S 3G9, Canada.

Kidney-on-a-chip devices may revolutionize the discovery of new therapies. However, fabricating a 3D glomerulus remains a challenge, due to a requirement for a microscale soft material with complex topography to support cell culture in a native configuration. Here, we describe the use of microfluidic spinning to recapitulate complex concave and convex topographies over multiple length scales, required for biofabrication of a biomimetic 3D glomerulus. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1021/acscentsci.9b01097DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7318083PMC

Conjugation of new DNA vaccine with polyethylenimine induces cellular immune response and tumor regression in neuroblastoma mouse model.

Exp Oncol 2020 06;42(2):120-125

Belarusian Research Center for Pediatric Oncology, Hematology and Immunology, Borovliany 220053, Belarus.

Aim: To estimate immunogenicity and antitumor effect of new DNA vaccine against neuroblastoma using tyrosine hydroxylase as an antigen and linear polyethylenimine (PEI) 20 kDa as a synthetic DNA carrier in syngeneic mouse tumor model.

Materials And Methods: DNA vaccine was made by cloning the tyrosine hydroxylase minigene fused to the potato virus X coat protein gene into the expression vector. The A/J mice were vaccinated by three intramuscular injections. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.32471/exp-oncology.2312-8852.vol-42-no-2.14473DOI Listing

Tetracenomycin X inhibits translation by binding within the ribosomal exit tunnel.

Nat Chem Biol 2020 Jun 29. Epub 2020 Jun 29.

Center of Life Sciences, Skolkovo Institute of Science and Technology, Skolkovo, Russia.

The increase in multi-drug resistant pathogenic bacteria is making our current arsenal of clinically used antibiotics obsolete, highlighting the urgent need for new lead compounds with distinct target binding sites to avoid cross-resistance. Here we report that the aromatic polyketide antibiotic tetracenomycin (TcmX) is a potent inhibitor of protein synthesis, and does not induce DNA damage as previously thought. Despite the structural similarity to the well-known translation inhibitor tetracycline, we show that TcmX does not interact with the small ribosomal subunit, but rather binds to the large subunit, within the polypeptide exit tunnel. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41589-020-0578-xDOI Listing