23,375 results match your criteria Biomolecules & Therapeutics[Journal]


Characterization, high production and antimicrobial activity of exopolysaccharides from Lactococcus lactis F-mou.

Microb Pathog 2019 Apr 16. Epub 2019 Apr 16.

Laboratory of Molecular Biology, Genomics and Bioinformatics, Faculty of Nature and Life Science, Hassiba Benbouali University, Chlef, Hay Essalam, 02000, Algeria.

A new exopolysaccharide (EPS) was produced by the Lactococcus lactis F-mou strain (LT898177.1) isolated from the Sahrawi camel milk in the Bir-Naam region, Algeria. The most influential production parameters were screened by the Plackett-Burman design for enhancing EPS yield utilizing the Mech-Degla juice as a low-cost raw material. Read More

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http://dx.doi.org/10.1016/j.micpath.2019.04.018DOI Listing

Multilayer nanoscale functionalisation to treat disorders and enhance regeneration of bone tissue.

Nanomedicine 2019 Apr 16. Epub 2019 Apr 16.

School of Engineering, Newcastle University, Claremont Road, Newcastle upon Tyne NE1 7RU, United Kingdom. Electronic address:

The coatings application onto medical devices has experienced a continuous growth in the last few years. Medical device coating market is expected to grow at a CAGR of 5.16% to reach USD 10 million by 2023 due to the increasing geriatric population and the growing demand for continuous innovation. Read More

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http://dx.doi.org/10.1016/j.nano.2019.03.009DOI Listing

Biomolecular Chemistry in Liquid Phase Separated Compartments.

Front Mol Biosci 2019 3;6:21. Epub 2019 Apr 3.

Institute for Molecules and Materials, Radboud University, Nijmegen, Netherlands.

Biochemical processes inside the cell take place in a complex environment that is highly crowded, heterogeneous, and replete with interfaces. The recently recognized importance of biomolecular condensates in cellular organization has added new elements of complexity to our understanding of chemistry in the cell. Many of these condensates are formed by liquid-liquid phase separation (LLPS) and behave like liquid droplets. Read More

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http://dx.doi.org/10.3389/fmolb.2019.00021DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6456709PMC

Liquid Biphasic Electric Partitioning System as a Novel Integration Process for Betacyanins Extraction From Red-Purple Pitaya and Antioxidant Properties Assessment.

Front Chem 2019 3;7:201. Epub 2019 Apr 3.

Bioseparation Research Group, Department of Chemical and Environmental Engineering, Faculty of Science and Engineering, University of Nottingham Malaysia, Semenyih, Malaysia.

Nowadays, downstream bioprocessing industries inclines towards the development of a green and high efficient bioseparation technology. Betacyanins are presently gaining higher interest in the food science as driven by their high tinctorial strength and health promoting functional properties. In this study, a novel green integration process of liquid biphasic electric partitioning system (LBEPS) was proposed for betacyanins extraction from peel and flesh of red-purple pitaya. Read More

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https://www.frontiersin.org/article/10.3389/fchem.2019.00201
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http://dx.doi.org/10.3389/fchem.2019.00201DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6456687PMC
April 2019
1 Read

Swarmer Cells Adaptation Toward UVc Radiations.

Front Microbiol 2019 2;10:556. Epub 2019 Apr 2.

Laboratoire de Traitement des Rejets Hydriques, Centre des Recherches et des Technologies des Eaux, Technopôle Borj Cedria, Nabeul, Tunisia.

Swarming is the most rapid surface motility allowing bacteria to rapidly colonize new surfaces. However, swarming behavior is affected by environmental factors like ultraviolet irradiation (UVc). UVc radiation is the most disinfection technology usually applied for wastewater and proven to be effective to inactivate microorganisms. Read More

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https://www.frontiersin.org/article/10.3389/fmicb.2019.00556
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http://dx.doi.org/10.3389/fmicb.2019.00556DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6454200PMC
April 2019
1 Read

Potential contribution of microRNA-125b targeting p38MAPK to relieving intermittent hypoxia-induced dementia of rat models.

J Clin Neurosci 2019 Apr 15. Epub 2019 Apr 15.

Department of Neurology, Shanghai Eighth People's Hospital, Shanghai 200235, China. Electronic address:

Allowing for the correlation between intermittent hypoxia and impaired cognition, we intended to figure out whether and how dementia-relevant biomolecules (e.g. miR-125b and p38MAPK) might participate in weakening of intelligence in the context of intermittent hypoxia. Read More

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http://dx.doi.org/10.1016/j.jocn.2019.03.002DOI Listing

The PTEN⁻PI3K Axis in Cancer.

Biomolecules 2019 Apr 17;9(4). Epub 2019 Apr 17.

Cancer Research Institute, Beth Israel Deaconess Cancer Center, Department of Medicine and Pathology, Beth Israel Deaconess Medical Center (BIDMC), Harvard Medical School, Boston, MA 02215, USA.

The PI3K-AKT-mTOR signal transduction pathway regulates a variety of biological processes including cell growth, cell cycle progression and proliferation, cellular metabolism, and cytoskeleton reorganization. Fine-tuning of the phosphatidylinositol 3-kinase (PI3K) pathway signaling output is essential for the maintenance of tissue homeostasis and uncontrolled activation of this cascade leads to a number of human pathologies including cancer. Inactivation of the tumor suppressor phosphatase and tensin homologue deleted on Chromosome 10 (PTEN) and/or activating mutations in the proto-typical lipid kinase PI3K have emerged as some of the most frequent events associated with human cancer and as a result the PI3K pathway has become a highly sought-after target for cancer therapies. Read More

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https://www.mdpi.com/2218-273X/9/4/153
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http://dx.doi.org/10.3390/biom9040153DOI Listing
April 2019
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Membrane Localization of HspA1A, a Stress Inducible 70-kDa Heat-Shock Protein, Depends on Its Interaction with Intracellular Phosphatidylserine.

Biomolecules 2019 Apr 17;9(4). Epub 2019 Apr 17.

Department of Biological Science, Center for Applied Biotechnology Studies, and Center for Computational and Applied Mathematics, College of Natural Sciences and Mathematics, California State University Fullerton, Fullerton, CA 92834-6850, USA.

HspA1A is a cytosolic molecular chaperone essential for cellular homeostasis. HspA1A also localizes at the plasma membrane (PM) of tumor and stressed cells. However, it is currently unknown how this cytosolic protein translocates to the PM. Read More

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http://dx.doi.org/10.3390/biom9040152DOI Listing

Regulation of the Expression of DAPK1 by SUMO Pathway.

Biomolecules 2019 Apr 17;9(4). Epub 2019 Apr 17.

Provincial University Key Laboratory of Cellular Stress Response and Metabolic Regulation, College of Life Sciences, Fujian Normal University, Fuzhou 350117, China.

Death Associated Protein Kinase 1 (DAPK1) is an important signaling kinase mediating the biological effect of multiple natural biomolecules such as IFN-γ, TNF-α, curcumin, etc. DAPK1 is degraded through both ubiquitin-proteasomal and lysosomal degradation pathways. To investigate the crosstalk between these two DAPK1 degradation pathways, we carried out a screen using a set of ubiquitin E2 siRNAs at the presence of Tuberous Sclerous 2 (TSC2) and identified that the small ubiquitin-like molecule (SUMO) pathway is able to regulate the protein levels of DAPK1. Read More

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https://www.mdpi.com/2218-273X/9/4/151
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http://dx.doi.org/10.3390/biom9040151DOI Listing
April 2019
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Application of conducting polymers to wound care and skin tissue engineering: A review.

Biosens Bioelectron 2019 Apr 6;135:50-63. Epub 2019 Apr 6.

Nanyang Environment and Water Research Institute, Residues and Resource Reclamation Center, 1 Cleantech Loop, CleanTech, Singapore, 637141, Singapore; School of Civil and Environmental Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore, 639798, Singapore. Electronic address:

The use of intrinsically conducting polymers (CPs) in wound care and skin tissue engineering presents a novel opportunity for accelerated wound healing, enhanced antibacterial activity and the potential for controlled drug delivery. Through their increased electrical conductivity, CPs can facilitate the application of electrical stimulation directly to the wound area, which has been linked to faster wound healing. The release of drugs or biological agents to the wound site can likewise be modulated through the use of an external electrical stimuli. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S09565663193028
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http://dx.doi.org/10.1016/j.bios.2019.04.001DOI Listing
April 2019
1 Read

Density artefacts at interfaces caused by multiple time-step effects in molecular dynamics simulations.

F1000Res 2018 5;7:1745. Epub 2018 Nov 5.

Laboratory of Physical Chemistry, ETH Zürich, Zurich, 8093, Switzerland.

Molecular dynamics (MD) simulations have become an important tool to provide insight into molecular processes involving biomolecules such as proteins, DNA, carbohydrates and membranes. As these processes cover a wide range of time scales, multiple time-step integration methods are often employed to increase the speed of MD simulations. For example, in the twin-range (TR) scheme, the nonbonded forces within the long-range cutoff are split into a short-range contribution updated every time step (inner time step) and a less frequently updated mid-range contribution (outer time step). Read More

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http://dx.doi.org/10.12688/f1000research.16715.1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6441880PMC
November 2018

Comparative genome mining and heterologous expression of an orphan NRPS gene cluster direct the production of ashimides.

Chem Sci 2019 Mar 21;10(10):3042-3048. Epub 2019 Jan 21.

State Key Laboratory of Pharmaceutical Biotechnology , Institute of Functional Biomolecules , School of Life Sciences , Nanjing University , 210023 , China . Email: ; Email:

The ever-increasing bacterial genomic repositories reveal a great number of uncharacterized biosynthetic gene clusters, representing a tremendous resource for natural product discovery. Genome mining of the marine sp. NA03103 indicates the presence of an orphan nonribosomal peptide synthetase (NRPS) gene cluster (), to which there are no homologous gene clusters in the public genome databases. Read More

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http://dx.doi.org/10.1039/c8sc05670fDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6427947PMC

Programmable intracellular DNA biocomputing circuits for reliable cell recognitions.

Chem Sci 2019 Mar 15;10(10):2989-2997. Epub 2019 Jan 15.

Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education) , College of Chemistry and Molecular Sciences , Wuhan University , Wuhan , P. R. China . Email:

Dynamic nucleic acid-based biocircuits have spurred substantial research efforts for diagnosis or biomedical applications at the molecular level; nevertheless, it still remains a challenge to design programmable molecular circuit devices for autonomous and accurate diagnosis of low abundance biomolecules in a complex intracellular environment. Herein, a reconfigurable hybridization-based chain reaction is introduced to assemble modular biocomputing circuits that include a general sensing module and a versatile processing module. By modular sensing module design, we realized multiple endogenous miRNA-initiated biocomputing operations, including binary logic gates (OR, AND, INHIBIT and XOR), and more advanced concatenated logic circuits (XOR-AND, XOR-INHIBIT, and XOR-OR) in different living cells. Read More

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http://dx.doi.org/10.1039/c8sc05217dDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6427941PMC

A rational quest for selectivity through precise ligand-positioning in tandem DNA-catalysed Friedel-Crafts alkylation/asymmetric protonation.

Chem Sci 2019 Mar 24;10(10):2875-2881. Epub 2019 Jan 24.

Institut des Biomolécules Max Mousseron , CNRS , Université de Montpellier , ENSCM , Place Eugène Bataillon , 34095 Montpellier , France . Email:

Covalent anchorage of a metallic co-factor to a DNA-based architecture is merely the only way to ensure an accurate positioning of a catalytic site within the chiral micro-environment offered by the DNA double helix. Ultimately, it also allows a fine-tuning of the catalytic pocket through simple synthetic modifications of the DNA sequence. Here, we report highly selective copper(ii)-catalysed asymmetric Friedel-Crafts conjugate addition/enantioselective protonation, which is due to a careful positioning of a bipyridine ligand within a DNA framework. Read More

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http://dx.doi.org/10.1039/c8sc05543bDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6429601PMC

Influence of infrared heating processing technology on the cooking characteristics and functionality of African legumes: a review.

J Food Sci Technol 2019 Apr 8;56(4):1669-1682. Epub 2019 Mar 8.

Department of Biotechnology and Food Technology, University of Johannesburg, P. O. Box 17011, Doornfontein, Johannesburg, 2028 South Africa.

African legumes are an important protein source in the human diet. However, a long and often extended cooking process has been identified as a major challenge in the consumption and utilisation of these legumes. The application of infrared heating as a method of shortening the cooking-time of African legume seeds and flour, by increasing their water absorption rates and pasting viscosity is emphasised in literature. Read More

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http://link.springer.com/10.1007/s13197-019-03661-5
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http://dx.doi.org/10.1007/s13197-019-03661-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6443696PMC
April 2019
1 Read

Intracellular Peptides in Cell Biology and Pharmacology.

Biomolecules 2019 Apr 16;9(4). Epub 2019 Apr 16.

Department of Pharmacology, Biomedical Sciences Institute, University of São Paulo (USP), São Paulo 05508-000, Brazil.

Intracellular peptides are produced by proteasomes following degradation of nuclear, cytosolic, and mitochondrial proteins, and can be further processed by additional peptidases generating a larger pool of peptides within cells. Thousands of intracellular peptides have been sequenced in plants, yeast, zebrafish, rodents, and in human cells and tissues. Relative levels of intracellular peptides undergo changes in human diseases and also when cells are stimulated, corroborating their biological function. Read More

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https://www.mdpi.com/2218-273X/9/4/150
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http://dx.doi.org/10.3390/biom9040150DOI Listing
April 2019
2 Reads

The evolution of proteome: from the primeval to the very dawn of LUCA.

Biosystems 2019 Apr 14. Epub 2019 Apr 14.

Theoretical Biology Group, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Ciudad de México CDMX, C.P. 04510, Mexico. Electronic address:

The attempt to delineate the essential features that characterized life in its beginnings and the understanding of how those features evolved, represent important scientific challenges. While there have been varied efforts in the elucidation of how the first biomolecules arose from a prebiotic environment, there remains important gaps towards the characterization of the complete repertoire of the Last Universal Common Ancestor (LUCA). We portray a step-wise proteome evolution, by looking at the phenotype encoded by each one of the genetic codes that were appearing along evolution, beginning with the primeval genetic code and then with two intermediate Extended RNA codes, which finally shaped the current Standard Genetic Code (SGC). Read More

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https://linkinghub.elsevier.com/retrieve/pii/S03032647193008
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http://dx.doi.org/10.1016/j.biosystems.2019.04.007DOI Listing
April 2019
1 Read

Optical multiplexed bioassays improve biomedical diagnostics.

Angew Chem Int Ed Engl 2019 Apr 17. Epub 2019 Apr 17.

CHINA.

Conventional analytical methods based on single detection to determine one disease marker are usually not sufficient and accurate because the progression of disease generally involves multiple chemicals and biomolecules. The drive for simultaneous analysis of multiple targets, which plays a key role in both basic biomedical research and clinical applications, demands the development of multiplexed bioassays with high-throughput. In this minireview, we systematically summarize the recent progress of optical multiplexed analytical techniques to improve biomedical diagnostics, in which fluorescence and surface enhanced Raman scattering (SERS) with distinctive optical features are chosen as the main readout signals. Read More

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http://dx.doi.org/10.1002/anie.201901964DOI Listing

Visible Light-Induced Sulfonylation/Arylation of Styrenes in a Double Radical Three-Component Photoredox Reaction.

Chemistry 2019 Apr 17. Epub 2019 Apr 17.

Universität Mainz, Institut für Organische Chemie, Duesbergweg 10-14, 55128, Mainz, GERMANY.

Simultaneous sulfonylation/arylation of styrene derivatives is achieved in a photoredox-catalyzed three-component reaction using visible light. A broad variety of difunctionalized products is accessible in mostly excellent yields and high diastereoselectivity. The developed reaction is scalable and suitable for the modification of styrene-functionalized biomolecules. Read More

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http://dx.doi.org/10.1002/chem.201901175DOI Listing

Ca-, Sr-, and Ba-Coordination polymers based on anthranilic acid via mechanochemistry.

Dalton Trans 2019 Apr 17. Epub 2019 Apr 17.

Department of Chemistry, Humboldt-Universität zu Berlin, Brook-Taylor-Str. 2, D-12489 Berlin, Germany.

Ca-, Sr-, and Ba-Based coordination polymers (CPs) were prepared mechanochemically by milling metal-hydroxide samples with anthranilic acid (oABAH). {[Ca(oABA)2(H2O)3]}n (1) consists of one-dimensional polymeric chains that are further connected by a hydrogen-bonding network. {[Sr(oABA)2(H2O)2]·H2O}n (2) is a one-dimensional CP in which water molecules bridge Sr2+ ions and increase the dimensionality by building an extended network. Read More

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http://xlink.rsc.org/?DOI=C9DT00991D
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http://dx.doi.org/10.1039/c9dt00991dDOI Listing
April 2019
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Catalytic mechanism and properties of pyridoxal 5'-phosphate independent racemases: how enzymes alter mismatched acidity and basicity.

Nat Prod Rep 2019 Apr 17. Epub 2019 Apr 17.

Department of Chemistry, University of Alberta, 11227 Saskatchewan Drive, Edmonton, Alberta, Canada T6G 2G2.

Covering: up to March 2019Amino acid racemases and epimerases are key enzymes that invert the configuration of common amino acids and supply many corresponding d-isomers in living organisms. Some d-amino acids are inherently bioactive, whereas others are building blocks for important biomolecules, for example lipid II, the bacterial cell wall precursor. Peptides containing them have enhanced proteolytic stability and can act as important recognition elements in mammalian systems. Read More

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http://dx.doi.org/10.1039/c9np00017hDOI Listing

Soft Matrix-Assisted Laser Desorption/Ionization for Labile Glycoconjugates.

J Am Soc Mass Spectrom 2019 Apr 16. Epub 2019 Apr 16.

Institute of Atomic and Molecular Sciences, Academia Sinica, P. O. Box 23-166, Taipei, 10617, Taiwan.

Since its introduction, matrix-assisted laser desorption/ionization (MALDI) has been widely used for the mass analysis of biomolecules. The "soft ionization" of MALDI enables accurate mass determination of intact biomolecules. However, the ionization and desorption processes of MALDI are not adequately soft as many labile biomolecules, such as glycoconjugates containing sialic acid or the sulfate functional group, easily dissociate into fragments and sometimes, no intact molecules are observed. Read More

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http://dx.doi.org/10.1007/s13361-019-02208-4DOI Listing

Environmentally Benign Carbon Nanodots Prepared from Lemon for the Sensitive and Selective Fluorescence Detection of Fe(III) and Tannic Acid.

J Fluoresc 2019 Apr 16. Epub 2019 Apr 16.

Department of Physical Chemistry, School of Chemistry, Madurai Kamaraj University, Madurai, Tamil Nadu, 625021, India.

Photoluminescent carbon nanodots (CNDs) were prepared using a biocarbon source of lemon extract. The obtained CNDs are of spherical shape and are enriched with the carboxylic acid fucntionalities. CNDs exhibited a fluorescence emission at 445 nm and unveiled blue luminescence in ultraviolet excitation. Read More

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http://dx.doi.org/10.1007/s10895-019-02360-wDOI Listing
April 2019
1.927 Impact Factor

Bactericidal effects and accelerated wound healing using TbO nanoparticles with intrinsic oxidase-like activity.

J Nanobiotechnology 2019 Apr 16;17(1):54. Epub 2019 Apr 16.

Division of Analytical Chemistry, Office of Regulatory Science, Center for Food Safety and Applied Nutrition, U.S. Food and Drug Administration, College Park, MD, 20740, USA.

Background: Nanomaterials that exhibit intrinsic enzyme-like characteristics have shown great promise as potential antibacterial agents. However, many of them exhibit inefficient antibacterial activity and biosafety problems that limit their usefulness. The development of new nanomaterials with good biocompatibility and rapid bactericidal effects is therefore highly desirable. Read More

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http://dx.doi.org/10.1186/s12951-019-0487-xDOI Listing

A Highly Efficient Indirect Surface Display Method Based on the CL7/Im7 Ultra-High-Affinity System.

Molecules 2019 Apr 15;24(8). Epub 2019 Apr 15.

State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan 430062, China.

Cell surface display systems for immobilization of peptides and proteins on the surface of cells have various applications, such as vaccine generation, protein engineering, bio-conversion and bio-adsorption. Though plenty of methods have been established in terms of traditional yeast surface display systems, the development of a universal display method with high efficiency remains a challenge. Here we report an indirect yeast surface display method by anchoring Im7 proteins on the surface of , achieving highly efficient display of target proteins, including fluorescence proteins (sfGFP and mCherry) or enzymes (human Arginase I), with a CL7 fusion tag through the ultra-high-affinity interaction between Im7 and CL7. Read More

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http://dx.doi.org/10.3390/molecules24081483DOI Listing

Is there a role for autophagy in ascending aortopathy associated with tricuspid or bicuspid aortic valve?

Clin Sci (Lond) 2019 Apr 2;133(7):805-819. Epub 2019 Apr 2.

Department of Translational Medical Sciences, Università degli Studi della Campania 'L. Vanvitelli', Naples, Italy.

Autophagy is a conserved process by which cytoplasmatic elements are sequestered in vesicles and degraded after their fusion with lysosomes, thus recycling the precursor molecules. The autophagy-mediated removal of redundant/harmful/damaged organelles and biomolecules plays not only a replenishing function, but protects against stressful conditions through an adaptive mechanism. Autophagy, known to play a role in several pathological conditions, is now gaining increasing attention also in the perspective of the identification of the pathogenetic mechanisms at the basis of ascending thoracic aortic aneurysm (TAA), a localized or diffused dilatation of the aorta with an abnormal widening greater than 50 percent of the vessel's normal diameter. Read More

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http://dx.doi.org/10.1042/CS20181092DOI Listing

Surface-Enhanced Raman Scattering Probing the Translocation of DNA and Amino Acid through Plasmonic Nanopores.

Anal Chem 2019 Apr 16. Epub 2019 Apr 16.

DNA and amino acid are important biomolecules in living organism. Probing such biomolecules with structural characters can provide valuable information for life study. Here, gold plasmonic nanopores (GPNs) with high SERS activity (a local enhancement factor higher than 10) are synthesized at the tip of a glass nanopipette. Read More

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http://dx.doi.org/10.1021/acs.analchem.9b01045DOI Listing

Label-free diagnosis of lung cancer with tissue-slice surface-enhanced Raman spectroscopy and statistical analysis.

Lasers Med Sci 2019 Apr 13. Epub 2019 Apr 13.

Shandong Province Key Laboratory of Medical Physics and Image Processing Technology, School of Physics and Electronics, Shandong Normal University, Jinan, 250014, China.

Despite the rapid development of medical science, the diagnosis of lung cancer is still quite challenging. Due to the ultrahigh detection sensitivity of surface-enhanced Raman spectroscopy (SERS), SERS has a broad application prospect in biomedicine, especially in the field of tumor blood detection. Although Raman spectroscopy can diagnose lung cancer through tissue slices, its weak cross sections are problematic. Read More

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http://dx.doi.org/10.1007/s10103-019-02781-wDOI Listing

Nanoscale magnetic imaging of ferritins in a single cell.

Sci Adv 2019 Apr 10;5(4):eaau8038. Epub 2019 Apr 10.

CAS Key Laboratory of Microscale Magnetic Resonance and Department of Modern Physics, University of Science and Technology of China, Hefei 230026, China.

The in situ measurement of the distribution of biomolecules inside a cell is one of the important goals in life science. Among various imaging techniques, magnetic imaging (MI) based on the nitrogen-vacancy (NV) center in diamond provides a powerful tool for the biomolecular research, while the nanometer-scale MI of intracellular proteins remains a challenge. Here, we use ferritin as a demonstration to realize the MI of endogenous proteins in a single cell using the NV center as the sensor. Read More

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http://dx.doi.org/10.1126/sciadv.aau8038DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6457937PMC
April 2019
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Stomatal Development and Perspectives toward Agricultural Improvement.

Cold Spring Harb Perspect Biol 2019 Apr 15. Epub 2019 Apr 15.

Institute of transformative Biomolecules (WPI-ITbM), Nagoya University, Chikusa, Nagoya 464-8601, Japan.

Stomata are small pores on the surface of land plants that facilitate gas exchange-acquiring CO from surrounding atmosphere and releasing water vapor. In adverse conditions, such as drought, stomata close to minimize water loss. The activities of stomata are vital for plant growth and survival. Read More

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http://dx.doi.org/10.1101/cshperspect.a034660DOI Listing

Stability of glyoxal- and methylglyoxal-modified hemoglobin on dried blood spot cards as analyzed by nanoflow liquid chromatography tandem mass spectrometry.

J Food Drug Anal 2019 Apr 27;27(2):526-530. Epub 2018 Oct 27.

Department of Chemistry and Biochemistry, National Chung Cheng University, 168 University Road, Ming-Hsiung, Chia-Yi 62142, Taiwan.

Blood sampling by the dried blood spot (DBS) technique has become commonly applied in newborn screening. It is often used for analysis of small molecules, such as metabolites. Recently, DBS sampling has been applied for quantification of post-translational protein modifications. Read More

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http://dx.doi.org/10.1016/j.jfda.2018.10.003DOI Listing

Membrane Lipidome Reorganization and Accumulation of Tissue DNA Lesions in Tumor-Bearing Mice: An Exploratory Study.

Cancers (Basel) 2019 Apr 4;11(4). Epub 2019 Apr 4.

Institute of Nanoscience and Nanotechnology, N.C.S.R. "Demokritos", 15310 Agia Paraskevi-Athens, Greece.

Increased rates of reactive oxygen/nitrogen species (ROS/RNS) are involved in almost all cancer types, associated with tumor development and progression, causing damage to biomolecules such as proteins, nucleic acids and membrane lipids, in different biological compartments. We used a human tumor xenograft mouse model to evaluate for the first time in parallel the remodeling of fatty acid moieties in erythrocyte membrane phospholipids and the level of ROS-induced DNA lesions in liver and kidney tissues. Using liquid chromatography tandem mass spectrometry the 5' and 5' diastereoisomers of 5',8-cyclo-2'-deoxyadenosine and 5',8-cyclo-2'-deoxyguanosine, together with 8-oxo-7,8-dihydro-2'-deoxyadenosine, were determined in mice at young (4- and 5-weeks) and old (17-weeks) ages and compared with control SCID mice without tumor implantation. Read More

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http://dx.doi.org/10.3390/cancers11040480DOI Listing

CRISPR/Cas9-Mediated Knock-Out of dUTPase in Mice Leads to Early Embryonic Lethality.

Biomolecules 2019 Apr 4;9(4). Epub 2019 Apr 4.

Institute of Enzymology, RCNS, Hungarian Academy of Sciences, H-1117 Budapest, Hungary.

Sanitization of nucleotide pools is essential for genome maintenance. Deoxyuridine 5'-triphosphate nucleotidohydrolase (dUTPase) is a key enzyme in this pathway since it catalyzes the cleavage of 2'-deoxyuridine 5'-triphosphate (dUTP) into 2'-deoxyuridine 5'-monophosphate (dUMP) and inorganic pyrophosphate. Through its action dUTPase efficiently prevents uracil misincorporation into DNA and at the same time provides dUMP, the substrate for de novo thymidylate biosynthesis. Read More

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http://dx.doi.org/10.3390/biom9040136DOI Listing

LcNAC13 Physically Interacts with LcR1MYB1 to Coregulate Anthocyanin Biosynthesis-Related Genes during Litchi Fruit Ripening.

Biomolecules 2019 Apr 4;9(4). Epub 2019 Apr 4.

Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China.

Anthocyanin accumulation is crucial for the development of quality for most fruit. The mechanism underlying the regulation of anthocyanin biosynthesis by transcription factors in litchi fruit remains largely unknown. In this study, we isolated one NAC (NAM, ATAF1/2 and CUC2) TF gene, . Read More

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http://dx.doi.org/10.3390/biom9040135DOI Listing

Preventive Effect of Anji White Tea Flavonoids on Alcohol-Induced Gastric Injury through Their Antioxidant Effects in Kunming Mice.

Biomolecules 2019 Apr 4;9(4). Epub 2019 Apr 4.

Chongqing Collaborative Innovation Center for Functional Food, Chongqing University of Education, Chongqing 400067, China.

Anji white tea () is a traditional Chinese tea beverage, which is classified as green tea and contains an abundant amount of flavonoids. In this study, the preventive effect of Anji white tea flavonoids (AJWTFs) on ethanol/hydrochloric acid-induced gastric injury in mice was evaluated. The serum and gastric tissues of mice were analyzed using a biochemical kit and by quantitative polymerase chain reaction (qPCR). Read More

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https://www.mdpi.com/2218-273X/9/4/137
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http://dx.doi.org/10.3390/biom9040137DOI Listing
April 2019
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Review of the Isolation, Characterization, Biological Function, and Multifarious Therapeutic Approaches of Exosomes.

Cells 2019 Apr 3;8(4). Epub 2019 Apr 3.

Department of Stem Cell and Regenerative Biotechnology, Konkuk University, 1 Hwayang-Dong, Gwangin-gu, Seoul 05029, Korea.

Exosomes are extracellular vesicles that contain a specific composition of proteins, lipids, RNA, and DNA. They are derived from endocytic membranes and can transfer signals to recipient cells, thus mediating a novel mechanism of cell-to-cell communication. They are also thought to be involved in cellular waste disposal. Read More

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http://dx.doi.org/10.3390/cells8040307DOI Listing

Evidence of Destabilization of the Human Thymidylate Synthase (hTS) Dimeric Structure Induced by the Interface Mutation Q62R.

Biomolecules 2019 Apr 3;9(4). Epub 2019 Apr 3.

Department of Biotechnology, Chemistry and Pharmacy-Department of Excellence 2018-2020, University of Siena, 53100 Siena, Italy.

In human cells, thymidylate synthase (TS) provides the only source of 2'-deoxythymidyne-5'-monophosphate (dTMP), which is required for DNA biosynthesis. Because of its pivotal role, human TS (hTS) represents a validated target for anticancer chemotherapy. Nonetheless, the efficacy of drugs blocking the hTS active site has limitations due to the onset of resistance in cancer cells, requiring the identification of new strategies to effectively inhibit this enzyme. Read More

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http://dx.doi.org/10.3390/biom9040134DOI Listing

Mutagenesis of DsbAss is Crucial for the Signal Recognition Particle Mechanism in : Insights from Molecular Dynamics Simulations.

Biomolecules 2019 Apr 3;9(4). Epub 2019 Apr 3.

Institute of Biochemistry and Biotechnology, Quaid-e-Azam Campus, University of the Punjab, Opp. Sheikh Zaid Hospital, Canal Bank Road, Lahore 54590, Pakistan.

The disulfide bond signal sequence (DsbAss) protein is characterized as an important virulence factor in gram-negative bacteria. This study aimed to analyze the "alanine" alteration in the hydrophobic (H) region of DsbAss and to understand the conformational DsbAss alteration(s) inside the fifty-four homolog (Ffh)-binding groove which were revealed to be crucial for translocation of ovine growth hormone (OGH) to the periplasmic space in via the secretory (Sec) pathway. An experimental design was used to explore the hydrophobicity and alteration of alanine (Ala) to isoleucine (Ile) in the tripartite structure of DsbAss. Read More

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http://dx.doi.org/10.3390/biom9040133DOI Listing

Poly(ethylene glycol)-block-poly(2-aminoethyl methacrylate hydrochloride)-based polyplexes as serum-tolerant nanosystems for enhanced gene delivery.

Mol Pharm 2019 Apr 15. Epub 2019 Apr 15.

PEGylation of cationic polyplexes has been used as a promising approach to enhance their stability and reduce unwanted interactions with biomolecules. However, this strategy generally has a negative effect on cellular uptake and, consequently, on transfection of target cells. In this work, we explore the impact of PEGylation on biological and physicochemical properties of poly(2-aminoethyl methacrylate) (PAMA)-based polyplexes. Read More

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http://dx.doi.org/10.1021/acs.molpharmaceut.9b00101DOI Listing

Proteomic Profiling during Infection Distinguishes the Intracellular Environment of Host Cells.

mSystems 2019 Mar-Apr;4(2). Epub 2019 Apr 9.

Institute of Analytical Chemistry and Synthetic and Functional Biomolecules Center, College of Chemistry and Molecular Engineering, Peking University, Beijing, People's Republic of China.

Essential to bacterial pathogenesis, Salmonella enterica serovar Typhimurium ( Typhimurium) has evolved the capacity to quickly sense and adapt to specific intracellular environment within distinct host cells. Here we examined Typhimurium proteomic remodeling within macrophages, allowing direct comparison with our previous studies in epithelial cells. In addition to many shared features, our data revealed proteomic signatures highly specific to one type of host cells. Read More

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http://dx.doi.org/10.1128/mSystems.00314-18DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6456673PMC
April 2019
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A Photochromic Azobenzene Peptidomimetic of a β-Turn Model Peptide Structure as a Conformational Switch.

Front Chem 2019 29;7:180. Epub 2019 Mar 29.

Laboratory of Peptide and Protein Chemistry and Biology (PeptLab), Sesto Fiorentino, Italy.

The insertion of azobenzene moiety in complex molecular protein or peptide systems can lead to molecular switches to be used to determine kinetics of folding/unfolding properties of secondary structures, such as α-helix, β-turn, or β-hairpin. In fact, in azobenzene, absorption of light induces a reversible trans ↔ cis isomerization, which in turns generates a strain or a structure relaxation in the chain that causes peptide folding/unfolding. In particular azobenzene may permit reversible conformational control of hairpin formation. Read More

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http://dx.doi.org/10.3389/fchem.2019.00180DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6449423PMC

Interactions of Transition Metal Dichalcogenide Nanosheets With Mucin: Quartz Crystal Microbalance With Dissipation, Surface Plasmon Resonance, and Spectroscopic Probing.

Front Chem 2019 29;7:166. Epub 2019 Mar 29.

State Key Laboratory of Chemical Engineering, Tianjin Key Laboratory of Membrane Science and Desalination Technology, School of Chemical Engineering and Technology, Tianjin University, Tianjin, China.

Ultrathin 2-dimensional transition metal dichalcogenides (TMDs) have become a class of high-potential materials in biomedicine due to their intriguing properties. They have been applied to solve biomedical challenges, such as biosensing, bioimaging, drug delivery, and cancer therapy. However, studies of the interactions between these materials and biomolecules are insufficient. Read More

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http://dx.doi.org/10.3389/fchem.2019.00166DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6449427PMC
March 2019
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A rapid MS/MS method to assess the deuterium kinetic isotope effect and associated improvement in the metabolic stability of deuterated biological and pharmacological molecules as applied to an imaging agent.

Anal Chim Acta 2019 Aug 22;1064:65-70. Epub 2019 Feb 22.

Australian Nuclear Science and Technology Organisation, Locked Bag 2001, Kirrawee DC, NSW, 2232, Australia. Electronic address:

The deuterium kinetic isotope effect has been known for a period of 40 years, but it is only relatively recently that new drug entities (NDEs) incorporating deuterium demonstrating beneficial pharmacokinetics, pharmacodynamics, and toxicology have arrived to market. Determination of the precise location to deuterate and subsequently any evaluation for a kinetic isotope effect (KIE) is challenging. Typically, such an evaluation would be performed in an in vitro metabolic assay (e. Read More

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http://dx.doi.org/10.1016/j.aca.2019.02.025DOI Listing

Characterization of iron nanoparticles/reduced graphene oxide composites synthesized by one step eucalyptus leaf extract.

Environ Pollut 2019 Apr 5;250:8-13. Epub 2019 Apr 5.

Fujian Key Laboratory of Pollution Control and Resource Reuse, School of Environmental Science and Engineering, Fujian Normal University, Fuzhou, 350007, Fujian Province, China. Electronic address:

Biosynthesis of nanoparticles using plant extract is one kind of the effective approach in developing rapid, clean, nontoxic, and eco-friendly technology. In this work, iron nanoparticles/reduced graphene oxide composites (Fe NPs/rGO) were facilely fabricated through one-step method with eucalyptus leaf extract. The synthesized Fe NPs/rGO was investigated by various characterization methods. Read More

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http://dx.doi.org/10.1016/j.envpol.2019.04.002DOI Listing

Autophagy as a molecular target for cancer treatment.

Eur J Pharm Sci 2019 Apr 11. Epub 2019 Apr 11.

Sabanci University Nanotechnology Research and Application Center (SUNUM), Istanbul 34956, Turkey. Electronic address:

Autophagy is an evolutionarily conserved catabolic mechanism, by which eukaryotic cells recycle or degrades internal constituents through membrane-trafficking pathway. Thus, autophagy provides the cells with a sustainable source of biomolecules and energy for the maintenance of homeostasis under stressful conditions such as tumor microenvironment. Recent findings revealed a close relationship between autophagy and malignant transformation. Read More

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http://dx.doi.org/10.1016/j.ejps.2019.04.011DOI Listing
April 2019
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Generalized Voigt broadening due to thermal fluctuations of electromechanical nanosensors and molecular electronic junctions.

J Chem Phys 2019 Apr;150(14):141102

Biophysics Group, Microsystems and Nanotechnology Division, Physical Measurement Laboratory, National Institute of Standards and Technology, Gaithersburg, Maryland 20899, USA.

Graphene and other 2D materials give a platform for electromechanical sensing of biomolecules in aqueous, room temperature environments. The electronic current changes in response to mechanical deflection, indicating the presence of forces due to interactions with, e.g. Read More

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http://aip.scitation.org/doi/10.1063/1.5081140
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http://dx.doi.org/10.1063/1.5081140DOI Listing
April 2019
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Theoretical investigation of size, shape, and aspect ratio effect on the LSPR sensitivity of hollow-gold nanoshells.

J Chem Phys 2019 Apr;150(14):144116

Department of Chemistry, Western Michigan University, Kalamazoo, Michigan 49008, USA.

The change in refractive index around plasmonic nanoparticles upon binding to biomolecules is routinely used in localized surface plasmon resonance (LSPR)-based biosensors and in biosensing platforms. In this study, the plasmon sensitivity of hollow gold (Au) nanoshells is studied using theoretical modeling where the influence of shape, size, shell thickness, and aspect ratio is addressed. Different shapes of hollow Au nanoshells are studied that include sphere, disk, triangular prism, rod, ellipsoid, and rectangular block. Read More

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http://dx.doi.org/10.1063/1.5090885DOI Listing

Incorporation of probiotic strain in raw minced beef meat: Study of textural modification, lipid and protein oxidation and color parameters during refrigerated storage.

Meat Sci 2019 Apr 4;154:29-36. Epub 2019 Apr 4.

Laboratory of Microorganisms and Biomolecules (LMB), Centre of Biotechnology of Sfax, Road of Sidi Mansour Km 6, P.O. Box 1177, Sfax 3018, Tunisia. Electronic address:

The aim of this work was to evaluate the effect of different concentrations of probiotic strain Lactobacillus plantarum TN8 on the quality and safety of raw minced beef after 10 days of refrigerated storage. The obtained results showed that the incorporation of the probiotic strain can inhibit the proliferation of spoilage microorganisms, such Listeria monocytogenes and Salmonella spp., delay the lipid oxidation, improve texture parameters, and extend the shelf life of these products during storage. Read More

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http://dx.doi.org/10.1016/j.meatsci.2019.04.005DOI Listing
April 2019
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Characterization and synergistic antibacterial potential of green synthesized silver nanoparticles using aqueous root extracts of important medicinal plants of Pakistan.

Colloids Surf B Biointerfaces 2019 Apr 8;179:317-325. Epub 2019 Apr 8.

Biotechnology Program, Department of Environmental Sciences, COMSATS University Islamabad, Abbottabad Campus, 22060, Abbottabad, Pakistan. Electronic address:

In the past few years, biologically synthesized silver nanoparticles (AgNPs) have been standout amongst the most utilized nanoparticles both in the field of therapeutics and clinical practices. Therefore, the current study aimed to synthesize AgNPs for the first time using aqueous root extracts of important plants of Pakistan i.e. Read More

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http://dx.doi.org/10.1016/j.colsurfb.2019.04.016DOI Listing

Intrinsically Disordered Proteins in Chronic Diseases.

Biomolecules 2019 Apr 11;9(4). Epub 2019 Apr 11.

Department of Molecular Medicine, Morsani College of Medicine, University of South Florida, Tampa, FL 33612, USA.

t is now increasingly evident that a large fraction of the human proteome comprises proteins that, under physiological conditions, lack fixed, ordered 3D structures as a whole or have segments that are not likely to form a defined 3D structure [... Read More

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http://dx.doi.org/10.3390/biom9040147DOI Listing