28,264 results match your criteria Biomolecules & Therapeutics[Journal]


Tailoring the surface properties and flexibility of starch-based films using oil and waxes recovered from potato chips byproducts.

Int J Biol Macromol 2020 Jun 29. Epub 2020 Jun 29.

LAQV-REQUIMTE, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, Portugal.

Agrofood byproducts may be exploited as a source of biomolecules suitable for developing bioplastic materials. In this work, the feasibility of using starch, oil, and waxes recovered from potato chips byproducts for films production was studied. The recovered potato starch-rich fraction (RPS) contained an amylopectin/amylose ratio of 2. Read More

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http://dx.doi.org/10.1016/j.ijbiomac.2020.06.231DOI Listing

A New Molecular-Mechanics Model for Simulations of Hydrogen Fluoride in Chemistry and Biology.

J Chem Theory Comput 2020 Jul 2. Epub 2020 Jul 2.

Hydrogen fluoride (HF) is the most polar diatomic molecule and one of the simplest molecules capable of hydrogen-bonding. HF deviates from ideality both in the gas phase and in solution, and is thus of great interest from a fundamental standpoint. Pure and aqueous HF solutions are also broadly used in chemical and industrial processes, despite their high toxicity. Read More

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http://dx.doi.org/10.1021/acs.jctc.0c00247DOI Listing

Plasmon-assisted grafting of anisotropic nanoparticles - spatially selective surface modification and the creation of amphiphilic SERS nanoprobes.

Nanoscale 2020 Jul 2. Epub 2020 Jul 2.

Department of Solid State Engineering, University of Chemistry and Technology, 16628 Prague, Czech Republic. and Research School of Chemistry and Applied Biomedical Sciences, Tomsk Polytechnic University, Russian Federation.

Amphiphilic nanoparticles (NPs) with a spatially selective distribution of grafted functional groups have great potential in the field of sensing, advanced imaging, and therapy due to their unique surface properties. The main techniques for the spatially selective functionalization of NPs utilize the surface-assisted approaches, which significantly restrict their production throughput. In this work, we propose an alternative plasmon-based route for the spatially selective grafting of anisotropic gold nanorods (AuNRs) using iodonium and diazonium salts. Read More

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http://dx.doi.org/10.1039/d0nr02934cDOI Listing

Botanical preparation HX109 inhibits macrophage-mediated activation of prostate epithelial cells through the CCL4-STAT3 pathway: implication for the mechanism underlying HX109 suppression of prostate hyperplasia.

Heliyon 2020 Jun 23;6(6):e04267. Epub 2020 Jun 23.

Helixmith Co. Ltd., 21, Magokjungang 8-ro 7-gil, Gangseo-gu, Seoul, 07794, Republic of Korea.

Benign prostatic hyperplasia (BPH) is one of the most frequently observed diseases in the elderly male population worldwide. A variety of factors such as aging, hormonal imbalance, chronic inflammation, and oxidative stress play an important role in its pathogenesis. We have previously shown that HX109, an ethanol extract prepared from 3 plants (, , and ), alleviates prostate hyperplasia in the BPH rat model and suppresses AR signaling by upregulating Ca/CAMKKβ and ATF3. Read More

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http://dx.doi.org/10.1016/j.heliyon.2020.e04267DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7322056PMC

MicroRNAs alteration as early biomarkers for cancer and neurodegenerative diseases: New challenges in pesticides exposure.

Toxicol Rep 2020 21;7:759-767. Epub 2020 May 21.

Department of Biomedical and Dental Sciences and Morphofunctional Imaging, Occupational Medicine Section, University of Messina, 98125, Messina, Italy.

This review summarizes the current knowledge linking cancer and neuro-degenerative diseases to dysregulation of microRNA network following pesticide exposure. Most findings revealed differential miRNA expression targeting biomolecules and pathways involved in various neoplastic localizations and neurodegenerative diseases. A growing body of evidence in recent literature indicates that alteration of specific miRNAs can represent an early biomarker of disease following exposure to chemical agents, including pesticides. Read More

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http://dx.doi.org/10.1016/j.toxrep.2020.05.003DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7322123PMC

The Red Color of Life Transformed - Synthetic Advances and Emerging Applications of Protoporphyrin IX in Chemical Biology.

European J Org Chem 2020 Jun 30;2020(22):3171-3191. Epub 2020 Mar 30.

School of Chemistry Trinity College Dublin The University of Dublin Trinity Biomedical Sciences Institute 152-160 Pearse Street 2 Dublin Ireland.

Protoporphyrin IX (PPIX) is the porphyrin scaffold of heme b, a ubiquitous prosthetic group of proteins responsible for oxygen binding (hemoglobin, myoglobin), electron transfer (cytochrome c) and catalysis (cytochrome P450, catalases, peroxidases). PPIX and its metallated derivatives frequently find application as therapeutic agents, imaging tools, catalysts, sensors and in light harvesting. The vast toolkit of accessible porphyrin functionalization reactions enables easy synthetic modification of PPIX to meet the requirements for its multiple uses. Read More

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http://dx.doi.org/10.1002/ejoc.202000074DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7319466PMC

Labeling Antibodies.

Cold Spring Harb Protoc 2020 Jul 1;2020(7):pdb.top099242. Epub 2020 Jul 1.

This introduction outlines general strategies for labeling proteins, with an emphasis on methods that are used primarily for labeling antibodies. It covers the specific site of modification, cross-linker options, types of labels, and postlabeling cleanup methodology, along with the advantages and disadvantages of each method. In general, polyclonal antibodies are more versatile and resistant to activity loss than are monoclonal antibodies. Read More

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http://dx.doi.org/10.1101/pdb.top099242DOI Listing

Bioactive Compounds from Larvae as Natural Ingredients for Cosmetic Application.

Biomolecules 2020 Jun 29;10(7). Epub 2020 Jun 29.

CBIOS-Research Center for Biosciences & Health Technologies, Universidade Lusófona, 1749-024 Lisboa, Portugal.

Due to the sustainable organic matter bioconversion process used as substrate for its development, the (Linnaeus) larvae biomass is considered a source of compounds with high aggregate value and quite a promising market. The materials that can be extracted from larvae have opened the door to a diverse new field of ingredients, mainly for the feed and food industry, but also with potential applicability in cosmetics. In this review we succinctly describe the larval development and rearing cycle, the main compounds identified from different types of extractions, their bioactivities and focus on possible applications in cosmetic products. Read More

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http://dx.doi.org/10.3390/biom10070976DOI Listing

Bioactive Surfaces vs. Conventional Surfaces in Titanium Dental Implants: A Comparative Systematic Review.

J Clin Med 2020 Jun 29;9(7). Epub 2020 Jun 29.

Department of Surgery, University of Salamanca, Instituto de Investigación Biomédica de Salamanca (IBSAL), 37007 Salamanca, Spain.

Animal studies and the scarce clinical trials available that have been conducted suggest that bioactive surfaces on dental implants could improve the osseointegration of such implants. The purpose of this systematic review was to compare the effectiveness of osseointegration of titanium (Ti) dental implants using bioactive surfaces with that of Ti implants using conventional surfaces such as sandblasted large-grit acid-etched (SLA) or similar surfaces. Applying the guidelines of the Preferred Reporting Items for Systematic Reviews and Meta-analyses (PRISMA) statement, the MEDLINE, PubMed Central and Web of Science databases were searched for scientific articles in April 2020. Read More

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http://dx.doi.org/10.3390/jcm9072047DOI Listing

Reduced Reelin Expression in the Hippocampus after Traumatic Brain Injury.

Biomolecules 2020 Jun 29;10(7). Epub 2020 Jun 29.

Department of Cell Biology and Neuroscience, Rutgers, the State University of New Jersey, Piscataway, NJ 08854, USA.

Traumatic brain injury (TBI) is a relatively common occurrence following accidents or violence, and often results in long-term cognitive or motor disability. Despite the high health cost associated with this type of injury, presently there are no effective treatments for many neurological symptoms resulting from TBI. This is due in part to our limited understanding of the mechanisms underlying brain dysfunction after injury. Read More

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http://dx.doi.org/10.3390/biom10070975DOI Listing

Application of Nanostructured Carbon-Based Electrochemical (Bio)Sensors for Screening of Emerging Pharmaceutical Pollutants in Waters and Aquatic Species: A Review.

Nanomaterials (Basel) 2020 Jun 29;10(7). Epub 2020 Jun 29.

REQUIMTE-LAQV, Instituto Superior de Engenharia do Porto, Instituto Politécnico do Porto, Rua Dr. António Bernardino de Almeida, 431, 4249-015 Porto, Portugal.

Pharmaceuticals, as a contaminant of emergent concern, are being released uncontrollably into the environment potentially causing hazardous effects to aquatic ecosystems and consequently to human health. In the absence of well-established monitoring programs, one can only imagine the full extent of this problem and so there is an urgent need for the development of extremely sensitive, portable, and low-cost devices to perform analysis. Carbon-based nanomaterials are the most used nanostructures in (bio)sensors construction attributed to their facile and well-characterized production methods, commercial availability, reduced cost, high chemical stability, and low toxicity. Read More

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http://dx.doi.org/10.3390/nano10071268DOI Listing

Transcriptome Based Profiling of the Immune Cell Gene Signature in Rat Experimental Colitis and Human IBD Tissue Samples.

Biomolecules 2020 Jun 29;10(7). Epub 2020 Jun 29.

Institute of Biochemistry, Biological Research Centre, 6726 Szeged, Hungary.

Chronic intestinal inflammation is characteristic of Inflammatory Bowel Disease (IBD) that is associated with the exaggerated infiltration of immune cells. A complex interplay of inflammatory mediators and different cell types in the colon are responsible for the maintenance of tissue homeostasis and affect pathological conditions. Gene expression alteration of colon biopsies from IBD patients and an rat model of colitis were examined by RNA-Seq and QPCR, while we used in silico methods, such as Ingenuity Pathway Analysis (IPA) application and the Immune Gene Signature (ImSig) package of R, to interpret whole transcriptome data and estimate immune cell composition of colon tissues. Read More

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http://dx.doi.org/10.3390/biom10070974DOI Listing

Protein-Based Nanoparticles as Drug Delivery Systems.

Pharmaceutics 2020 Jun 29;12(7). Epub 2020 Jun 29.

Department of Biotechnology and Bioengineering, Kangwon National University, Chuncheon 24341, Korea.

Nanoparticles have been extensively used as carriers for the delivery of chemicals and biomolecular drugs, such as anticancer drugs and therapeutic proteins. Natural biomolecules, such as proteins, are an attractive alternative to synthetic polymers commonly used in nanoparticle formulation because of their safety. In general, protein nanoparticles offer many advantages, such as biocompatibility and biodegradability. Read More

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http://dx.doi.org/10.3390/pharmaceutics12070604DOI Listing

Martini Coarse-Grained Model of Hyaluronic Acid for the Structural Change of Its Gel in the Presence of Monovalent and Divalent Salts.

Int J Mol Sci 2020 Jun 29;21(13). Epub 2020 Jun 29.

Department of Physics and Research Institute of Natural Science, Gyeongsang National University (GNU), 501 Jinju-daero, Jinju 52828, Korea.

Hyaluronic acid (HA) has a wide range of biomedical applications including the formation of hydrogels, microspheres, sponges, and films. The modeling of HA to understand its behavior and interaction with other biomolecules at the atomic level is of considerable interest. The atomistic representation of long HA polymers for the study of the macroscopic structural formation and its interactions with other polyelectrolytes is computationally demanding. Read More

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http://dx.doi.org/10.3390/ijms21134602DOI Listing

Magnetic nanowires in biomedical applications.

Nanotechnology 2020 Jul 1. Epub 2020 Jul 1.

Wuhan University of Science and Engineering, Wuhan, 430200, CHINA.

Magnetic nanostructures and nanomaterials play essential roles in modern bio medicine and technology. Proper surface functionalization of nanoparticles (NPs) allows the selective bonding thus application of magnetic forces to a vast range of cellular structures and biomolecules. However, the spherical geometry of NPs poises a series of limitations in various potential applications. Read More

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http://dx.doi.org/10.1088/1361-6528/aba1baDOI Listing

Extending the Debye scattering equation for diffraction from a cylindrically averaged group of atoms: detecting molecular orientation at an interface.

Acta Crystallogr A Found Adv 2020 Jul 20;76(Pt 4):468-473. Epub 2020 May 20.

School of Physics A28, The University of Sydney, NSW 2006, Australia.

The Debye scattering equation is now over 100 years old and has been widely used to interpret diffraction patterns from randomly oriented groups of atoms. The present work develops and applies a related equation that calculates diffraction intensity from groups of atoms randomly oriented about a fixed axis, a scenario that occurs when molecules are oriented at an interface by the presentation of a binding motif as in antibody binding. Using an example biomolecule, the high level of sensitivity of the diffraction pattern to the orientation of the molecule and to the direction of the incident beam is shown. Read More

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http://dx.doi.org/10.1107/S2053273320005276DOI Listing

THE IMS PARADOX: A PERSPECTIVE ON STRUCTURAL ION MOBILITY-MASS SPECTROMETRY.

Mass Spectrom Rev 2020 Jul 1. Epub 2020 Jul 1.

Department of Chemistry, Texas A&M University, College Station, TX, 77843.

Studies of large proteins, protein complexes, and membrane protein complexes pose new challenges, most notably the need for increased ion mobility (IM) and mass spectrometry (MS) resolution. This review covers evolutionary developments in IM-MS in the authors' and key collaborators' laboratories with specific focus on developments that enhance the utility of IM-MS for structural analysis. IM-MS measurements are performed on gas phase ions, thus "structural IM-MS" appears paradoxical-do gas phase ions retain their solution phase structure? There is growing evidence to support the notion that solution phase structure(s) can be retained by the gas phase ions. Read More

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http://dx.doi.org/10.1002/mas.21642DOI Listing

Microbiological quality and safety of commercialized thalassotherapy products based on marine mud and algae extracts in Tunisia.

Arch Microbiol 2020 Jun 30. Epub 2020 Jun 30.

Laboratory of Microorganisms and Active Biomolecules, MBA-LR03ES03, Faculty of Sciences of Tunis, University of Tunis El Manar, Tunis, Tunisia.

A total of 15 samples of thalassotherapy products, distributed in Tunisia in their intact and final state of production, was analyzed to determine their microbiological safety status. The result shows the absence of pathogenic bacteria (Staphylococcus aureus, Candida albicans, Salmonella, Pseudomonas aeruginosa and coliforms). The incidence of contamination by Gram-positive Bacilli (mesophelic bacteria, aerobic and anaerobic spore forming bacteria, anaerobic sulphite-reducing bacteria) was found to be higher in products composed by mud and extract of alga. Read More

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http://dx.doi.org/10.1007/s00203-020-01957-1DOI Listing

Chrysomycin A Derivatives for the Treatment of Multi-Drug-Resistant Tuberculosis.

ACS Cent Sci 2020 Jun 4;6(6):928-938. Epub 2020 May 4.

Beijing National Laboratory for Molecular Sciences, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, Department of Chemical Biology, College of Chemistry and Molecular Engineering, Synthetic and Functional Biomolecules Center, and Peking-Tsinghua Center for Life Sciences, Peking University, Beijing 100871, China.

Tuberculosis (TB) is a life-threatening disease resulting in an estimated 10 million new infections and 1.8 million deaths annually, primarily in underdeveloped countries. The economic burden of TB has been estimated as approximately 12 billion USD annually in direct and indirect costs. Read More

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http://dx.doi.org/10.1021/acscentsci.0c00122DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7318084PMC

A Practical Perspective on the Roles of Solution NMR Spectroscopy in Drug Discovery.

Molecules 2020 Jun 28;25(13). Epub 2020 Jun 28.

Experimental Drug Development Centre (EDDC), Agency for Science, Technology and Research (A*STAR), 10 Biopolis Road, Chromos, #05-01, Singapore 138670, Singapore.

Solution nuclear magnetic resonance (NMR) spectroscopy is a powerful tool to study structures and dynamics of biomolecules under physiological conditions. As there are numerous NMR-derived methods applicable to probe protein-ligand interactions, NMR has been widely utilized in drug discovery, especially in such steps as hit identification and lead optimization. NMR is frequently used to locate ligand-binding sites on a target protein and to determine ligand binding modes. Read More

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http://dx.doi.org/10.3390/molecules25132974DOI Listing

Physicochemical Properties and Liposomal Formulations of Hydrolysate Fractions of Four Sea Cucumbers (Holothuroidea: Echinodermata) from the Northwestern Algerian Coast.

Molecules 2020 Jun 28;25(13). Epub 2020 Jun 28.

Laboratoire d'Ingénierie des Biomolécules (LIBio), Lorraine University, 2, Forêt de Haye avenue TSA 40602, 54518 Vandœuvre CEDEX, France.

To promote the nutritional and pharmacological values of four sea cucumber species ( and ), harvested from the Algerian coast, we aimed to study their proximate composition, fatty acid profile and angiotensin-converting enzyme (ACE) inhibitory activity. Their phospholipids were also used to elaborate nanoliposomes and to encapsulate peptides obtained from the same source. After the physico-chemical characterization of nanoliposomes and peptides, in vitro analyses were realized. Read More

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http://dx.doi.org/10.3390/molecules25132972DOI Listing

Few-Layer Graphene-Based Nanofluids with Enhanced Thermal Conductivity.

Nanomaterials (Basel) 2020 Jun 28;10(7). Epub 2020 Jun 28.

Laboratoire de Génie Civil et Génie Mécanique, Université de Rennes, F-35000 Rennes, France.

High-quality graphene is an especially promising carbon nanomaterial for developing nanofluids for enhancing heat transfer in fluid circulation systems. We report a complete study on few layer graphene (FLG) based nanofluids, including FLG synthesis, FLG-based nanofluid preparation, and their thermal conductivity. The FLG sample is synthesized by an original mechanical exfoliation method. Read More

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http://dx.doi.org/10.3390/nano10071258DOI Listing

Mesothelin-Targeted Recombinant Immunotoxins for Solid Tumors.

Biomolecules 2020 Jun 28;10(7). Epub 2020 Jun 28.

Laboratory of Molecular Biology, Center for Cancer Research, NCI, NIH, Bethesda, MD 20892, USA.

Mesothelin (MSLN) is a cell surface glycoprotein normally expressed only on serosal surfaces, and not found in the parenchyma of vital organs. Many solid tumors also express MSLN, including mesothelioma and pancreatic adenocarcinoma. Due to this favorable expression profile, MSLN represents a viable target for directed anti-neoplastic therapies, such as recombinant immunotoxins (iToxs). Read More

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http://dx.doi.org/10.3390/biom10070973DOI Listing

Evaluation of Resin-Based Material Containing Copaiba Oleoresin ( Ducke): Biological Effects on the Human Dental Pulp Stem Cells.

Biomolecules 2020 Jun 28;10(7). Epub 2020 Jun 28.

Department of Restorative Dentistry, School of Dentistry, University of Sao Paulo, São Paulo, SP 05508-060, Brazil.

The purpose of this study was to analyze in vitro the biological effects on human dental pulp stem cells triggered in response to substances leached or dissolved from two experimental cements for dental pulp capping. The experimental materials, based on extracts from Ducke (COP), were compared to calcium hydroxide [Ca(OH)] and mineral trioxide aggregate (MTA), materials commonly used for direct dental pulp capping in restorative dentistry. For this, human dental pulp stem cells were exposed to COP associated or not with Ca(OH) or MTA. Read More

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http://dx.doi.org/10.3390/biom10070972DOI Listing

Validation of a Novel Zebrafish Model of Dengue Virus (DENV-3) Pathology Using the Pentaherbal Medicine Denguenil Vati.

Biomolecules 2020 Jun 28;10(7). Epub 2020 Jun 28.

Drug Discovery and Development Division, Patanjali Research Institute, NH-58, Haridwar 249 405, Uttarakhand, India.

Dengue is a devastating viral fever of humans, caused by dengue virus. Using a novel zebrafish model of dengue pathology, we validated the potential anti-dengue therapeutic properties of pentaherbal medicine, Denguenil Vati. At two different time points (at 7 and 14 days post infection with dengue virus), we tested three translational doses (5. Read More

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http://dx.doi.org/10.3390/biom10070971DOI Listing

Development and Characterization of a Fucoidan-Based Drug Delivery System by Using Hydrophilic Anticancer Polysaccharides to Simultaneously Deliver Hydrophobic Anticancer Drugs.

Biomolecules 2020 Jun 28;10(7). Epub 2020 Jun 28.

Department of Materials Science and Engineering, National Chiao Tung University, Hsinchu 30010, Taiwan.

Fucoidan, a natural sulfated polysaccharide, which can activate the immune response and lessen adverse effects, is expected to be an adjuvant agent in combination with chemotherapy. Using natural hydrophilic anticancer polysaccharides to simultaneously encapsulate hydrophobic anticancer drugs is feasible, and a reduced side effect can be achieved to amplify the therapeutic efficacy. In this study, a novel type of fucoidan-PLGA nanocarrier (FPN-DTX) was developed for the encapsulation of the hydrophobic anticancer drug, docetaxel (DTX), as a drug delivery system. Read More

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http://dx.doi.org/10.3390/biom10070970DOI Listing

TOP mRNPs: Molecular Mechanisms and Principles of Regulation.

Biomolecules 2020 Jun 27;10(7). Epub 2020 Jun 27.

Brigham and Women's Hospital, Harvard Medical School, Harvard Initiative for RNA Medicine, Boston, MA 02115, USA.

The cellular response to changes in the surrounding environment and to stress requires the coregulation of gene networks aiming to conserve energy and resources. This is often achieved by downregulating protein synthesis. The 5' Terminal OligoPyrimidine (5' TOP) motif-containing mRNAs, which encode proteins that are essential for protein synthesis, are the primary targets of translational control under stress. Read More

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http://dx.doi.org/10.3390/biom10070969DOI Listing

Co-Delivery of Imiquimod and Curcumin by Nanoemugel for Improved Topical Delivery and Reduced Psoriasis-Like Skin Lesions.

Biomolecules 2020 Jun 27;10(7). Epub 2020 Jun 27.

Department of Pharmaceutics, College of Pharmacy, Najran University, Najran 11001, Saudi Arabia.

The current investigation aimed to improve the topical efficacy of imiquimod in combination with curcumin using the nanoemulsion-based delivery system through a combinatorial approach. Co-delivery of curcumin acts as an adjuvant therapeutic and to minimize the adverse skin reactions that are frequently associated with the topical therapy of imiquimod for the treatment of cutaneous infections and basal cell carcinomas. The low-energy emulsification method was used for the nano-encapsulation of imiquimod and curcumin in the nanodroplet oil phase, which was stabilized using Tween 20 in an aqueous dispersion system. Read More

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http://dx.doi.org/10.3390/biom10070968DOI Listing

Transcriptome Analysis Reveals Potential Roles of Abscisic Acid and Polyphenols in Adaptation of to Extreme Environmental Conditions in the Qinghai-Tibetan Plateau.

Biomolecules 2020 Jun 26;10(6). Epub 2020 Jun 26.

State Key Laboratory of Plateau Ecology and Agriculture, Qinghai University, Xining 810016, China.

A detailed understanding of the molecular mechanisms of plant stress resistance in the face of ever-changing environmental stimuli will be helpful for promoting the growth and production of crop and forage plants. Investigations of plant responses to various single abiotic or biotic factors, or combined stresses, have been extensively reported. However, the molecular mechanisms of plants in responses to environmental stresses under natural conditions are not clearly understood. Read More

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http://dx.doi.org/10.3390/biom10060967DOI Listing

Encapsulins-Bacterial Protein Nanocompartments: Structure, Properties, and Application.

Biomolecules 2020 Jun 26;10(6). Epub 2020 Jun 26.

Laboratory "Biomedical Nanomaterials", National University of Science and Technology "MISiS", Leninskiy Prospect, 4, 119049 Moscow, Russia.

Recently, a new class of prokaryotic compartments, collectively called encapsulins or protein nanocompartments, has been discovered. The shell proteins of these structures self-organize to form icosahedral compartments with a diameter of 25-42 nm, while one or more cargo proteins with various functions can be encapsulated in the nanocompartment. Non-native cargo proteins can be loaded into nanocompartments and the surface of the shells can be further functionalized, which allows for developing targeted drug delivery systems or using encapsulins as contrast agents for magnetic resonance imaging. Read More

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http://dx.doi.org/10.3390/biom10060966DOI Listing

Vibrational Spectroscopy of Peritoneal Dialysis Effluent for Rapid Assessment of Patient Characteristics.

Biomolecules 2020 Jun 26;10(6). Epub 2020 Jun 26.

Christian Doppler Laboratory for Molecular Stress Research in Peritoneal Dialysis, Department of Pediatrics and Adolescent Medicine, Medical University of Vienna, 1090 Vienna, Austria.

Peritoneal dialysis (PD) offers specific advantages over hemodialysis, enabling increased autonomy of patients with end-stage renal disease, but PD-related complications need to be detected in a timely manner. Fourier transform infrared (FTIR) spectroscopy could provide rapid and essential insights into the patients' risk profiles via molecular fingerprinting of PD effluent, an abundant waste material that is rich in biological information. In this study, we measured FTIR spectroscopic profiles in PD effluent from patients taking part in a randomized controlled trial of alanyl-glutamine addition to the PD-fluid. Read More

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http://dx.doi.org/10.3390/biom10060965DOI Listing

Reelin Functions, Mechanisms of Action and Signaling Pathways During Brain Development and Maturation.

Authors:
Yves Jossin

Biomolecules 2020 Jun 26;10(6). Epub 2020 Jun 26.

Laboratory of Mammalian Development & Cell Biology, Institute of Neuroscience, Université Catholique de Louvain, 1200 Brussels, Belgium.

During embryonic development and adulthood, Reelin exerts several important functions in the brain including the regulation of neuronal migration, dendritic growth and branching, dendritic spine formation, synaptogenesis and synaptic plasticity. As a consequence, the Reelin signaling pathway has been associated with several human brain disorders such as lissencephaly, autism, schizophrenia, bipolar disorder, depression, mental retardation, Alzheimer's disease and epilepsy. Several elements of the signaling pathway are known. Read More

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http://dx.doi.org/10.3390/biom10060964DOI Listing

Concise Polygenic Models for Cancer-Specific Identification of Drug-Sensitive Tumors from Their Multi-Omics Profiles.

Biomolecules 2020 Jun 26;10(6). Epub 2020 Jun 26.

Cancer Research Center of Marseille, INSERM U1068, F-13009 Marseille, France.

In silico models to predict which tumors will respond to a given drug are necessary for Precision Oncology. However, predictive models are only available for a handful of cases (each case being a given drug acting on tumors of a specific cancer type). A way to generate predictive models for the remaining cases is with suitable machine learning algorithms that are yet to be applied to existing in vitro pharmacogenomics datasets. Read More

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http://dx.doi.org/10.3390/biom10060963DOI Listing

Rapid deployment of smartphone-based augmented reality tools for field and online education in structural biology.

Biochem Mol Biol Educ 2020 Jun 30. Epub 2020 Jun 30.

Department of Genetics, Cell Biology, and Development, University of Minnesota, Minneapolis, Minnesota, USA.

Structural biology education commonly employs molecular visualization software, such as PyMol, RasMol, and VMD, to allow students to appreciate structure-function relationships in biomolecules. In on-ground, classroom-based education, these programs are commonly used on University-owned devices with software preinstalled. Remote education typically involves the use of student-owned devices, which complicates the use of such software, owing to the fact that (a) student devices have differing configurations (e. Read More

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http://dx.doi.org/10.1002/bmb.21396DOI Listing

Detection of Collagen-IV Using Highly Reflective Metal Nanoparticles-Immobilized Photosensitive Optical Fiber-Based MZI Structure.

IEEE Trans Nanobioscience 2020 Jul;19(3):477-484

In this work, a photosensitive (PS) optical fiber-based Mach-Zehnder interferometer (MZI) structure is developed to diagnose the presence of collagen-IV in human bodies. The MZI is fabricated by sequentially splicing the single mode-multimode-photosensitive-multimode-single mode (SMPMS) fiber segments. The sensing region in MZI structure is created by partially removing the cladding of photosensitive fiber by using 40% hydrofluoric (HF) acid and depositing the layers of highly reflective metal nanoparticles (NPs) over it. Read More

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http://dx.doi.org/10.1109/TNB.2020.2998520DOI Listing

Cryogenic OrbiSIMS Localizes Semi-Volatile Molecules in Biological Tissues.

Angew Chem Int Ed Engl 2020 Jun 30. Epub 2020 Jun 30.

Francis Crick Institute, Laboratory of Physiology and Metabolism, 1 Midland Road, NW1 1AT, London, UNITED KINGDOM.

OrbiSIMS is a recently developed instrument for label-free imaging of chemicals with micron spatial resolution and high mass resolution. We report a cryogenic workflow for OrbiSIMS (Cryo-OrbiSIMS) that improves chemical detection of lipids and other biomolecules in tissues. Cryo-OrbiSIMS boosts ionization yield and decreases ion-beam induced fragmentation, greatly improving the detection of biomolecules such as triacylglycerides. Read More

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http://dx.doi.org/10.1002/anie.202006881DOI Listing

Ultrasensitive Detection of Attomolar Protein Concentrations by Dropcast Single Molecule Assays.

J Am Chem Soc 2020 Jun 30. Epub 2020 Jun 30.

Department of Pathology, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts 02115, United States.

Measurements of very low levels of biomolecules, including proteins and nucleic acids, remain a critical challenge in many clinical diagnostic applications due to insufficient sensitivity. While digital measurement methods such as Single Molecule Arrays (Simoa), or digital ELISA, have made significant advances in sensitivity, there are still many potential disease biomarkers that exist in accessible biofluids at levels below the detection limits of these techniques. To overcome this barrier, we have developed a simple strategy for single molecule counting, dropcast single molecule assays (dSimoa), that enables more target molecules to be counted through increased sampling efficiency and with a simpler workflow. Read More

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http://dx.doi.org/10.1021/jacs.0c04331DOI Listing

Measurement of Enzymatic and Nonenzymatic Polyunsaturated Fatty Acid Oxidation Products in Plasma and Urine of Macular Degeneration Using LC-QTOF-MS/MS.

Lipids 2020 Jun 30. Epub 2020 Jun 30.

School of Biological Sciences, The University of Hong Kong, Pokfulam Road, Pokfulam Road, Hong Kong, Hong Kong SAR.

Oxidized polyunsaturated fatty acids (PUFA) are associated to pathogenesis of diseases including cardiovascular and neurodegeneration. The novel products are not only biomarkers but also lipid mediators in gene regulation and signaling pathways. Herein, simultaneous quantitation of 28 products derived from nonenzymatic and enzymatic oxidation of PUFA i. Read More

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http://dx.doi.org/10.1002/lipd.12264DOI Listing

Moving forward with isoprostanes, neuroprostanes and phytoprostanes: where are we now?

Essays Biochem 2020 Jun 30. Epub 2020 Jun 30.

Institut des Biomolécules Max Mousseron (IBMM), UMR 5247 CNRS, ENSCM, Université de Montpellier, France.

Polyunsaturated fatty acids (PUFAs) are essential components in eukaryotic cell membrane. They take part in the regulation of cell signalling pathways and act as precursors in inflammatory metabolism. Beside these, PUFAs auto-oxidize through free radical initiated mechanism and release key products that have various physiological functions. Read More

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http://dx.doi.org/10.1042/EBC20190096DOI Listing

Recent advances in transition metal-mediated modification of biomolecules.

Chemistry 2020 Jun 30. Epub 2020 Jun 30.

Universidade da Coruna, Química, SPAIN.

The site-selective modification of biomolecules has grown spectacularly during recent years. The presence of a large number of functional groups in a biomolecule makes its chemo- and regioselective modification a challenging goal. In this context, transition metal-mediated reactions are emerging as a powerful tool owing to their unique reactivity and good functional group compatibility, allowing highly efficient and selective bioconjugation reactions that operate under mild conditions. Read More

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http://dx.doi.org/10.1002/chem.202001287DOI Listing

Enzymatic degradation of liquid droplets of DNA is modulated near the phase boundary.

Proc Natl Acad Sci U S A 2020 Jun 29. Epub 2020 Jun 29.

Physics Department, Ludwig-Maximilians University, 80539 Munich, Germany.

Biomolecules can undergo liquid-liquid phase separation (LLPS), forming dense droplets that are increasingly understood to be important for cellular function. Analogous systems are studied as early-life compartmentalization mechanisms, for applications as protocells, or as drug-delivery vehicles. In many of these situations, interactions between the droplet and enzymatic solutes are important to achieve certain functions. Read More

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http://dx.doi.org/10.1073/pnas.2001654117DOI Listing

Reliable Quantification of pH Variation in Live Cells Using Prussian Blue-Caged SERS Probes.

Anal Chem 2020 Jun 30. Epub 2020 Jun 30.

Intracellular pH is an important parameter that is highly associated with diverse physiological processes. The reliable measurement of pH values inside cells remains a formidable challenge because of the complexity of cytoplasm. Herein, we report a robust Prussian blue (PB)-caged pH-responsive surface-enhanced Raman scattering (SERS) probe for precisely mapping the dynamic pH values in live cells. Read More

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http://dx.doi.org/10.1021/acs.analchem.0c00714DOI Listing

Class A G Protein-Coupled Receptor Antagonist Famotidine as a Therapeutic Alternative Against SARS-CoV2: An In Silico Analysis.

Biomolecules 2020 Jun 24;10(6). Epub 2020 Jun 24.

Department of Pharmacology, Cleveland Center for Membrane and Structural Biology, School of Medicine, Case Western Reserve University, Cleveland, OH 44106, USA.

The pandemic associated with Severe Acute Respiratory Syndrome Coronavirus type 2 (SARS-CoV2) and its disease named COVID-19 challenged the scientific community to discover effective therapeutic solutions in a short period. Repurposing existing drugs is one viable approach that emphasizes speed during these urgent times. Famotidine, a class A G protein-coupled receptor antagonist used for the treatment of gastroesophageal reflux was recently identified in an in silico screening. Read More

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http://dx.doi.org/10.3390/biom10060954DOI Listing

Pathogenesis of Preeclampsia and Therapeutic Approaches Targeting the Placenta.

Biomolecules 2020 Jun 24;10(6). Epub 2020 Jun 24.

Department of Obstetrics and Gynecology, UMKC School of Medicine, Kansas City, MO 64108, USA.

Preeclampsia (PE) is a serious pregnancy complication, affecting about 5-7% of pregnancies worldwide and is characterized by hypertension and damage to multiple maternal organs, primarily the liver and kidneys. PE usually begins after 20 weeks' gestation and, if left untreated, can lead to serious complications and lifelong disabilities-even death-in both the mother and the infant. As delivery is the only cure for the disease, treatment is primarily focused on the management of blood pressure and other clinical symptoms. Read More

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http://dx.doi.org/10.3390/biom10060953DOI Listing

The Citrus Flavonoid Hesperetin Has an Inadequate Anti-Arrhythmic Profile in the ΔKPQ Na1.5 Mutant of the Long QT Type 3 Syndrome.

Biomolecules 2020 Jun 24;10(6). Epub 2020 Jun 24.

Laboratory of Ion Channel Research, Department of Cellular and Molecular Medicine, KU Leuven, VIB Center for Brain & Disease Research, 3000 Leuven, Belgium.

Type 3 long QT syndromes (LQT3) are associated with arrhythmogenic gain-of-function mutations in the cardiac voltage-gated Na channel (hNa1.5). The citrus flavanone hesperetin (HSP) was previously suggested as a template molecule to develop new anti-arrhythmic drugs, as it blocks slowly-inactivating currents carried by the LQT3-associated hNa1. Read More

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http://dx.doi.org/10.3390/biom10060952DOI Listing

Coassemble Dopamine and GHK Tripeptide into Fluorescent Nanoparticles for pH Sensing.

Luminescence 2020 Jun 29. Epub 2020 Jun 29.

Biomedical Nanocenter, School of Life Science, Inner Mongolia Agricultural University, 29 East Erdos Street, Hohhot, P. R. China.

Fluorescent nanostructures have been widely applied to biomedical researches and clinical diagnosis such as biolabeling/imaging/sensing and have even acted as therapy reagents. Peptide-based fluorescent nanostructures attract recent interest from biomedical researchers. Inspired by the natural existence of GHK-Cu complex with a growth factor-like effect in human blood, here we have developed a novel approach for designing nanosensors through the co-assembling of two kinds of biomolecules. Read More

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http://dx.doi.org/10.1002/bio.3907DOI Listing

Biosynthesis, characterization and mechanism of formation of ZnO nanoparticles using Petroselinum crispum leaf extract.

Curr Org Synth 2020 Jun 28. Epub 2020 Jun 28.

Dept. of IT, Soran Technical Institute, Erbil Polytechnic University, Soran-Erbil, Kurdistan Region. Iraq.

Aims: Synthesizing ZnO NPs using Petroselinum crispum extract, commonly known as parsley, as a source of biosynthesis without utilizing chemical agents for reducing, capping and stabilizing agent.

Background: Recently, the biosynthesis of nanoparticles has been widely explored due to the wide range of vital applications in nanotechnology. Biosynthesized zinc oxide nanoparticles, ZnO NPs, has become increasingly important since it has many applications and environmentally friendly. Read More

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http://dx.doi.org/10.2174/1570179417666200628140547DOI Listing

Structural predictions of the functions of membrane proteins from HDX-MS.

Biochem Soc Trans 2020 Jun 29. Epub 2020 Jun 29.

Department of Chemistry, King's College London, London, U.K.

HDX-MS has emerged as a powerful tool to interrogate the structure and dynamics of proteins and their complexes. Recent advances in the methodology and instrumentation have enabled the application of HDX-MS to membrane proteins. Such targets are challenging to investigate with conventional strategies. Read More

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http://dx.doi.org/10.1042/BST20190880DOI Listing

Elastic and Thermoreversible Iongels by Supramolecular PVA/Phenol Interactions.

Macromol Biosci 2020 Jun 28:e2000119. Epub 2020 Jun 28.

Instituto de Desarrollo Tecnológico para la Industria Química (INTEC) CONICET, Güemes 3450, Santa Fe, 3000, Argentina.

Iongels have attracted much attention over the years as ion-conducting soft materials for applications in several technologies including stimuli-responsive drug release and flexible (bio)electronics. Nowadays, iongels with additional functionalities such as electronic conductivity, self-healing, thermo-responsiveness, or biocompatibility are actively being searched for high demanding applications. In this work, a simple and rapid synthetic pathway to prepare elastic and thermoreversible iongels is presented. Read More

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http://dx.doi.org/10.1002/mabi.202000119DOI Listing