758 results match your criteria Biomolecules[Journal]


CRISPR/Cas9-Mediated Knock-Out of dUTPase in Mice Leads to Early Embryonic Lethality.

Biomolecules 2019 Apr 4;9(4). Epub 2019 Apr 4.

Institute of Enzymology, RCNS, Hungarian Academy of Sciences, H-1117 Budapest, Hungary.

Sanitization of nucleotide pools is essential for genome maintenance. Deoxyuridine 5'-triphosphate nucleotidohydrolase (dUTPase) is a key enzyme in this pathway since it catalyzes the cleavage of 2'-deoxyuridine 5'-triphosphate (dUTP) into 2'-deoxyuridine 5'-monophosphate (dUMP) and inorganic pyrophosphate. Through its action dUTPase efficiently prevents uracil misincorporation into DNA and at the same time provides dUMP, the substrate for de novo thymidylate biosynthesis. Read More

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http://dx.doi.org/10.3390/biom9040136DOI Listing

LcNAC13 Physically Interacts with LcR1MYB1 to Coregulate Anthocyanin Biosynthesis-Related Genes during Litchi Fruit Ripening.

Biomolecules 2019 Apr 4;9(4). Epub 2019 Apr 4.

Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China.

Anthocyanin accumulation is crucial for the development of quality for most fruit. The mechanism underlying the regulation of anthocyanin biosynthesis by transcription factors in litchi fruit remains largely unknown. In this study, we isolated one NAC (NAM, ATAF1/2 and CUC2) TF gene, . Read More

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http://dx.doi.org/10.3390/biom9040135DOI Listing

Preventive Effect of Anji White Tea Flavonoids on Alcohol-Induced Gastric Injury through Their Antioxidant Effects in Kunming Mice.

Biomolecules 2019 Apr 4;9(4). Epub 2019 Apr 4.

Chongqing Collaborative Innovation Center for Functional Food, Chongqing University of Education, Chongqing 400067, China.

Anji white tea () is a traditional Chinese tea beverage, which is classified as green tea and contains an abundant amount of flavonoids. In this study, the preventive effect of Anji white tea flavonoids (AJWTFs) on ethanol/hydrochloric acid-induced gastric injury in mice was evaluated. The serum and gastric tissues of mice were analyzed using a biochemical kit and by quantitative polymerase chain reaction (qPCR). Read More

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https://www.mdpi.com/2218-273X/9/4/137
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http://dx.doi.org/10.3390/biom9040137DOI Listing
April 2019
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Evidence of Destabilization of the Human Thymidylate Synthase (hTS) Dimeric Structure Induced by the Interface Mutation Q62R.

Biomolecules 2019 Apr 3;9(4). Epub 2019 Apr 3.

Department of Biotechnology, Chemistry and Pharmacy-Department of Excellence 2018-2020, University of Siena, 53100 Siena, Italy.

In human cells, thymidylate synthase (TS) provides the only source of 2'-deoxythymidyne-5'-monophosphate (dTMP), which is required for DNA biosynthesis. Because of its pivotal role, human TS (hTS) represents a validated target for anticancer chemotherapy. Nonetheless, the efficacy of drugs blocking the hTS active site has limitations due to the onset of resistance in cancer cells, requiring the identification of new strategies to effectively inhibit this enzyme. Read More

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http://dx.doi.org/10.3390/biom9040134DOI Listing

Mutagenesis of DsbAss is Crucial for the Signal Recognition Particle Mechanism in : Insights from Molecular Dynamics Simulations.

Biomolecules 2019 Apr 3;9(4). Epub 2019 Apr 3.

Institute of Biochemistry and Biotechnology, Quaid-e-Azam Campus, University of the Punjab, Opp. Sheikh Zaid Hospital, Canal Bank Road, Lahore 54590, Pakistan.

The disulfide bond signal sequence (DsbAss) protein is characterized as an important virulence factor in gram-negative bacteria. This study aimed to analyze the "alanine" alteration in the hydrophobic (H) region of DsbAss and to understand the conformational DsbAss alteration(s) inside the fifty-four homolog (Ffh)-binding groove which were revealed to be crucial for translocation of ovine growth hormone (OGH) to the periplasmic space in via the secretory (Sec) pathway. An experimental design was used to explore the hydrophobicity and alteration of alanine (Ala) to isoleucine (Ile) in the tripartite structure of DsbAss. Read More

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http://dx.doi.org/10.3390/biom9040133DOI Listing

Intrinsically Disordered Proteins in Chronic Diseases.

Biomolecules 2019 Apr 11;9(4). Epub 2019 Apr 11.

Department of Molecular Medicine, Morsani College of Medicine, University of South Florida, Tampa, FL 33612, USA.

t is now increasingly evident that a large fraction of the human proteome comprises proteins that, under physiological conditions, lack fixed, ordered 3D structures as a whole or have segments that are not likely to form a defined 3D structure [... Read More

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http://dx.doi.org/10.3390/biom9040147DOI Listing

Recent Advances in Computational Protocols Addressing Intrinsically Disordered Proteins.

Biomolecules 2019 Apr 11;9(4). Epub 2019 Apr 11.

Center for Theoretical Biological Physics, Rice University, Houston, TX 77030, USA.

Intrinsically disordered proteins (IDP) are abundant in the human genome and have recently emerged as major therapeutic targets for various diseases. Unlike traditional proteins that adopt a definitive structure, IDPs in free solution are disordered and exist as an ensemble of conformations. This enables the IDPs to signal through multiple signaling pathways and serve as scaffolds for multi-protein complexes. Read More

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http://dx.doi.org/10.3390/biom9040146DOI Listing

Essential Kinases and Transcriptional Regulators and Their Roles in Autoimmunity.

Biomolecules 2019 Apr 10;9(4). Epub 2019 Apr 10.

Department of Internal Medicine, Ohio State University College of Medicine and Wexner Medical Center, Columbus, OH 43201, USA.

Kinases and transcriptional regulators are fundamental components of cell signaling that are expressed on many types of immune cells which are involved in secretion of cytokines, cell proliferation, differentiation, and apoptosis. Both play important roles in biological responses in health as well as in illnesses such as the autoimmune diseases which comprise at least 80 disorders. These diseases are caused by complex genetic and environmental interactions that lead to a breakage of immunologic tolerance and a disruption of the balance between self-reactive cells and regulatory cells. Read More

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http://dx.doi.org/10.3390/biom9040145DOI Listing

Host-Microbiome Synergistic Control on Sphingolipid Metabolism by Mechanotransduction in Model Arthritis.

Biomolecules 2019 Apr 9;9(4). Epub 2019 Apr 9.

CAS-MPG Partner Institute for Computational Biology, Shanghai Institutes for Biological Sciences, Shanghai 200031, China.

Chronic inflammatory autoimmune disorders are systemic diseases with increasing incidence and still lack a cure. More recently, attention has been placed in understanding gastrointestinal (GI) dysbiosis and, although important progress has been made in this area, it is currently unclear to what extent microbiome manipulation can be used in the treatment of autoimmune disorders. Via the use of appropriate models, rheumatoid arthritis (RA), a well-known exemplar of such pathologies, can be exploited to shed light on the currently overlooked effects of existing therapies on the GI microbiome. Read More

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http://dx.doi.org/10.3390/biom9040144DOI Listing
April 2019
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Impact of rs12075 Variants on Liver Fibrosis Progression in Patients with Chronic Hepatitis C: A Retrospective Study.

Biomolecules 2019 Apr 9;9(4). Epub 2019 Apr 9.

Unidad de Infección Viral e Inmunidad, Centro Nacional de Microbiología, Instituto de Salud Carlos III, 28220 Majadahonda, Spain.

The Duffy antigen receptor for chemokines rs12075 polymorphism regulates leukocyte trafficking and proinflammatory chemokine homeostasis. Hepatitis C virus (HCV)-mediated liver fibrosis is associated with an uncontrolled inflammatory response. In this study, we evaluate the association between the rs12075 polymorphism and liver stiffness progression in HCV-infected patients. Read More

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http://dx.doi.org/10.3390/biom9040143DOI Listing
April 2019
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Receptor Ligands as Helping Hands to L-DOPA in the Treatment of Parkinson's Disease.

Biomolecules 2019 Apr 9;9(4). Epub 2019 Apr 9.

Scuola di Scienze del Farmaco e dei Prodotti della Salute, Università di Camerino, Via S. Agostino 1, 62032 Camerino, Italy.

Levodopa (LD) is the most effective drug in the treatment of Parkinson's disease (PD). However, although it represents the "gold standard" of PD therapy, LD can cause side effects, including gastrointestinal and cardiovascular symptoms as well as transient elevated liver enzyme levels. Moreover, LD therapy leads to LD-induced dyskinesia (LID), a disabling motor complication that represents a major challenge for the clinical neurologist. Read More

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http://dx.doi.org/10.3390/biom9040142DOI Listing

Unilateral Ureteral Obstruction as a Model to Investigate Fibrosis-Attenuating Treatments.

Biomolecules 2019 04 8;9(4). Epub 2019 Apr 8.

Department of Biology, Faculty of Chemistry, National Autonomous University of Mexico (UNAM), Mexico City 04510, Mexico.

Renal fibrosis is the common pathway for most forms of progressive renal disease. The Unilateral Ureteral Obstruction (UUO) model is used to cause renal fibrosis, where the primary feature of UUO is tubular injury as a result of obstructed urine flow. Furthermore, experimental UUO in rodents is believed to mimic human chronic obstructive nephropathy in an accelerated manner. Read More

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https://www.mdpi.com/2218-273X/9/4/141
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http://dx.doi.org/10.3390/biom9040141DOI Listing
April 2019
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Organellar DNA Polymerases in Complex Plastid-Bearing Algae.

Biomolecules 2019 04 7;9(4). Epub 2019 Apr 7.

Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8572, Japan.

DNA replication in plastids and mitochondria is generally regulated by nucleus-encoded proteins. In plants and red algae, a nucleus-encoded enzyme called POP (plant and protist organellar DNA polymerase) is involved in DNA replication in both organelles by virtue of its dual localization. POPs are family A DNA polymerases, which include bacterial DNA polymerase I (PolI). Read More

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http://dx.doi.org/10.3390/biom9040140DOI Listing

Freeze Tolerance in Sculpins (Pisces; Cottoidea) Inhabiting North Pacific and Arctic Oceans: Antifreeze Activity and Gene Sequences of the Antifreeze Protein.

Biomolecules 2019 04 6;9(4). Epub 2019 Apr 6.

Usujiri Fisheries Station, Field Science Center of Northern Biosphere, Hokkaido University, Hakodate 041-1613, Japan.

Many marine species inhabiting icy seawater produce antifreeze proteins (AFPs) to prevent their body fluids from freezing. The sculpin species of the superfamily Cottoidea are widely found from the Arctic to southern hemisphere, some of which are known to express AFP. Here we clarified DNA sequence encoding type I AFP for 3 species of 2 families (Cottidae and Agonidae) belonging to Cottoidea. Read More

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http://dx.doi.org/10.3390/biom9040139DOI Listing

Essential Oils and Isolated Terpenes in Nanosystems Designed for Topical Administration: A Review.

Biomolecules 2019 04 5;9(4). Epub 2019 Apr 5.

Programa de Pós-Graduação em Ciências Farmacêuticas, Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, Av. Ipiranga, 2752, Porto Alegre 90610-000, Brazil.

Essential oils are natural products with a complex composition. Terpenes are the most common class of chemical compounds present in essential oils. Terpenes and the essential oils containing them are widely used and investigated by their pharmacological properties and permeation-enhancing ability. Read More

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http://dx.doi.org/10.3390/biom9040138DOI Listing

Helical Twist and Rotational Forces in the Mitotic Spindle.

Biomolecules 2019 04 1;9(4). Epub 2019 Apr 1.

Department of Physics, Faculty of Science, University of Zagreb, Bijenička cesta 32, 10000 Zagreb, Croatia.

The mitotic spindle segregates chromosomes into two daughter cells during cell division. This process relies on the precise regulation of forces acting on chromosomes as the cell progresses through mitosis. The forces in the spindle are difficult to directly measure using the available experimental techniques. Read More

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http://dx.doi.org/10.3390/biom9040132DOI Listing

Splicing Factors Have an Essential Role in Prostate Cancer Progression and Androgen Receptor Signaling.

Biomolecules 2019 04 1;9(4). Epub 2019 Apr 1.

Department of Functional Biogerontology, Tokyo Metropolitan Institute of Gerontology, 35-2 Sakae-cho, Itabashi-ku, Tokyo 173-0015, Japan.

Although inhibition of the androgen⁻androgen receptor (AR) axis effectively represses the growth of prostate cancer, most of all cases eventually become castration-resistant prostate cancers (CRPCs). Enhancement of the expression of AR and its variants along with the downstream signals is important for disease progression. AR-V7, a constitutive active form of AR, is generated as a result of RNA splicing. Read More

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http://dx.doi.org/10.3390/biom9040131DOI Listing

Study on Differential Protein Expression in Natural Selenium-Enriched and Non-Selenium-Enriched Rice Based on iTRAQ Quantitative Proteomics.

Biomolecules 2019 03 30;9(4). Epub 2019 Mar 30.

Demonstration Base for International Science & Technology Cooperation of Sichuan Province, Rice Research Institute, Sichuan Agricultural University, Chengdu 611130, Sichuan, China.

This work was designated to scrutinize the protein differential expression in natural selenium-enriched and non-selenium-enriched rice using the Isobaric-tags for relative and absolute quantification (iTRAQ) proteomics approach. The extracted proteins were subjected to enzyme digestion, desalting, and identified by iTRAQ coupled with liquid chromatography-tandem mass spectrometry (LC-MS/MS) technology. High pH C18 separation analysis was performed, and the data were then analyzed by Protein Pilot (V4. Read More

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http://dx.doi.org/10.3390/biom9040130DOI Listing

Matrix Metalloproteinase 9 as a Predictor of Coronary Atherosclerotic Plaque Instability in Stable Coronary Heart Disease Patients with Elevated Lipoprotein(a) Levels.

Biomolecules 2019 03 29;9(4). Epub 2019 Mar 29.

Federal State Budget Institution "National Cardiology Research Center" of Ministry of Health of the Russian Federation; 15A, 3d Cherepkovskaya street, 121552 Moscow, Russia.

We sought to investigate whether levels of matrix metalloproteinases (MMPs) and their inhibitors predict coronary atherosclerotic plaque instability, as assessed by intravascular ultrasound (IVUS) virtual histology during coronary angiography. Blood samples were collected before angiography in 32 subjects (mean age 56 ± 8 years) with stable coronary heart disease (CHD) and elevated lipoprotein(a) (Lp(a), 94 ± 35 mg/dL). Levels of high-sensitivity C-reactive protein (hsCRP), apolipoprotein B100 (apoB100), MMP-7, MMP-9, tissue inhibitor of metalloproteinases (TIMP)-1, and TIMP-2 were determined using commercially available enzyme-linked immunosorbent assay kits. Read More

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http://dx.doi.org/10.3390/biom9040129DOI Listing

Structural Stabilization of Human Transthyretin by (L.) Urban Extract: Implications for TTR Amyloidosis.

Biomolecules 2019 03 29;9(4). Epub 2019 Mar 29.

Department of Biochemistry, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand.

Transthyretin is responsible for a series of highly progressive, degenerative, debilitating, and incurable protein misfolding disorders known as transthyretin (TTR) amyloidosis. Since dissociation of the homotetrameric protein to its monomers is crucial in its amyloidogenesis, stabilizing the native tetramer from dissociating using small-molecule ligands has proven a viable therapeutic strategy. The objective of this study was to determine the potential role of the medicinal herb on human transthyretin (huTTR) amyloidogenesis. Read More

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http://dx.doi.org/10.3390/biom9040128DOI Listing

Antioxidant, Anti-Lung Cancer, and Anti-Bacterial Activities of .

Biomolecules 2019 03 29;9(4). Epub 2019 Mar 29.

Department of Medical Biotechnology, College of Biomedical Sciences, Kangwon National University, Chuncheon, Gangwon 24341, Korea.

This work tested antioxidant, anti-lung cancer, and antibacterial activities by in vitro, in vivo, and computational experiments for the metabolites extracted from the bark, seed, and stem of . The results showed that all the extracts significantly scavenged 1,2-diphenyl-1-picrylhydrazyl (DPPH) in a dose-dependent manner. But, the total phenol content (TPC) ranged from 2. Read More

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https://www.mdpi.com/2218-273X/9/4/127
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http://dx.doi.org/10.3390/biom9040127DOI Listing
March 2019
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Greensporone A, a Fungal Secondary Metabolite Suppressed Constitutively Activated AKT via ROS Generation and Induced Apoptosis in Leukemic Cell Lines.

Biomolecules 2019 03 29;9(4). Epub 2019 Mar 29.

Translational Research Institute, Academic Health System, Hamad Medical Corporation, P.O. Box 3050, Doha, Qatar.

Greensporone A is a fungal secondary metabolite that has exhibited potential in vitro for anti-proliferative activity in vitro. We studied the anticancer activity of greensporone A in a panel of leukemic cell lines. Greensporone A-mediated inhibition of proliferation is found to be associated with the induction of apoptotic cell death. Read More

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http://dx.doi.org/10.3390/biom9040126DOI Listing

Cytosporone B as a Biological Preservative: Purification, Fungicidal Activity and Mechanism of Action against .

Biomolecules 2019 03 29;9(4). Epub 2019 Mar 29.

Key Laboratory of Plant Resource Conservation and Sustainable Utilization, Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China.

To prevent citrus decay caused by , 12 natural products were isolated from two endophytic fungi, in which cytosporone B was shown to have excellent bioactivity for control of with median effect concentration (EC) of 26.11 μg/mL and minimum inhibitory concentration (MIC) of 105 μg/mL, and also significantly reduced the decay of sugar orange during the in vivo trials. In addition, cytosporone B could alter the morphology of by causing distortion of the mycelia and loss of membrane integrity. Read More

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http://dx.doi.org/10.3390/biom9040125DOI Listing

Differential Effects of Yeast NADH Dehydrogenase (Ndi1) Expression on Mitochondrial Function and Inclusion Formation in a Cell Culture Model of Sporadic Parkinson's Disease.

Biomolecules 2019 03 27;9(4). Epub 2019 Mar 27.

Parkinson's and Movement Disorders Center, Virginia Commonwealth University, P.O. Box 980539, Richmond, VA 23298, USA.

Parkinson's disease (PD) is a neurodegenerative disorder that exhibits aberrant protein aggregation and mitochondrial dysfunction. Ndi1, the yeast mitochondrial NADH dehydrogenase (complex I) enzyme, is a single subunit, internal matrix-facing protein. Previous studies have shown that Ndi1 expression leads to improved mitochondrial function in models of complex I-mediated mitochondrial dysfunction. Read More

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http://dx.doi.org/10.3390/biom9040119DOI Listing
March 2019
2 Reads

Comparison of the Neuroprotective Effects of Aspirin, Atorvastatin, Captopril and Metformin in Diabetes Mellitus.

Biomolecules 2019 03 27;9(4). Epub 2019 Mar 27.

Neurogenic Inflammation Research Center, Mashhad University of Medical Sciences, Mashhad 9177948564, Iran.

Objective: The aim of this study was to investigate the effect of combined intake of a high dose of aspirin, atorvastatin, captopril and metformin on oxidative stress in the brain cortex and hippocampus of streptozotocin (STZ)-induced diabetic rats.

Material And Methods: Rats were randomly divided into the following 11 groups: control and diabetic (D), as well as 9 groups that were treated with metformin (M, 300 mg/kg) or aspirin (ASA, 120 mg/kg) alone or in different combinations with captopril (C, 50 mg/kg) and/or atorvastatin (AT, 40 mg/kg) as follows: (D + M), (D + ASA), (D + M + ASA), (D + M + C), (D + M + AT), (D + M + C + ASA), (D + M + C + AT), (D + M + AT + ASA) and (D + M + C + AT + ASA). The rats in treatment groups received drugs by gavage daily for six weeks. Read More

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http://dx.doi.org/10.3390/biom9040118DOI Listing
March 2019
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Inhibition of Oncogenic Kinases: An In Vitro Validated Computational Approach Identified Potential Multi-Target Anticancer Compounds.

Biomolecules 2019 03 28;9(4). Epub 2019 Mar 28.

Institute of Pharmaceutical Sciences, Riphah University, 54000 Lahore, Pakistan.

Tumorigenesis in humans is a multistep progression that imitates genetic changes leading to cell transformation and malignancy. Oncogenic kinases play a central role in cancer progression, rendering them putative targets for the design of anti-cancer drugs. The presented work aims to identify the potential multi-target inhibitors of oncogenic receptor tyrosine kinases (RTKs) and serine/threonine kinases (STKs). Read More

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http://dx.doi.org/10.3390/biom9040124DOI Listing

Evaluation of Cyclosaplin Efficacy Using a Silk Based 3D Tumor Model.

Biomolecules 2019 03 28;9(4). Epub 2019 Mar 28.

Plant Biotechnology Laboratory, Department of Biotechnology, Indian Institute of Technology Kharagpur, Kharagpur-721302, West Bengal, India.

Development of novel anti-cancer peptides requires a rapid screening process which can be accelerated by using appropriate in vitro tumor models. Breast carcinoma tissue is a three-dimensional (3D) microenvironment, which contains a hypoxic center surrounded by dense proliferative tissue. Biochemical clues provided by such a 3D cell mass cannot be recapitulated in conventional 2D culture systems. Read More

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http://dx.doi.org/10.3390/biom9040123DOI Listing
March 2019
1 Read

Evaluation and Behavior of Spanish Bodybuilders: Doping and Sports Supplements.

Biomolecules 2019 03 28;9(4). Epub 2019 Mar 28.

Faculty of Health Sciences of Universidad Isabel I, Universidad Isabel I, 09004 Burgos, Spain.

The use of doping agents has these days become a public health problem, as it also affects young and non-competitive amateurs in different sports. To prepare for competition, bodybuilders perform aggressive dietary protocols, so, bodybuilders frequently consume nutritional supplements (NS) and banned substances in large dosages. Thus, the aim of this study is to analyze the prevalence of banned substances consumption and NS intake in competitive level bodybuilders. Read More

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http://dx.doi.org/10.3390/biom9040122DOI Listing

Quantification of Fecal Short Chain Fatty Acids by Liquid Chromatography Tandem Mass Spectrometry-Investigation of Pre-Analytic Stability.

Biomolecules 2019 03 28;9(4). Epub 2019 Mar 28.

Institute for Clinical Chemistry and Laboratory Medicine, University Hospital Regensburg, Franz-Josef-Strauss-Allee 11, 93053 Regensburg, Germany.

Short chain fatty acids (SCFAs) are generated by the degradation and fermentation of complex carbohydrates, (i.e., dietary fiber) by the gut microbiota relevant for microbe⁻host communication. Read More

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http://dx.doi.org/10.3390/biom9040121DOI Listing
March 2019
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Combining Autoclaving with Mild Alkaline Solution as a Pretreatment Technique to Enhance Glucose Recovery from the Invasive Weed .

Biomolecules 2019 03 28;9(4). Epub 2019 Mar 28.

Department of Biology, Faculty of Science, Naresuan University, Muang, Phitsanulok 65000, Thailand.

Developing an optimum pretreatment condition to enhance glucose recovery assessed the potential of , which is a common invasive weed in Thailand, as a feedstock for bioethanol production. was exposed to autoclave-assisted alkaline pretreatment by using different sodium hydroxide (NaOH) concentrations (1% to 4%) and heat intensities (110 °C to 130 °C) that were dissipated from autoclaving. The optimum condition for pretreatment was determined to be 2% NaOH at 110 °C for 60 min. Read More

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http://dx.doi.org/10.3390/biom9040120DOI Listing

Structure and Ligands Interactions of Tryptophan Decarboxylase by Molecular Modeling and Docking Simulations.

Biomolecules 2019 03 26;9(3). Epub 2019 Mar 26.

CNR, IBBR, 80055 Portici, Italy.

In a previous work, we in silico annotated protein sequences of genus plants as putative tryptophan decarboxylase (pTDC). Here, we investigated the structural properties of pTDCs by using the TDC sequence of as an experimentally annotated reference to carry out comparative modeling and substrate docking analyses. The functional annotation as TDC was verified by combining 3D molecular modeling and docking simulations, evidencing the peculiarities and the structural similarities with TDC. Read More

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http://dx.doi.org/10.3390/biom9030117DOI Listing

Electrostatics of Tau Protein by Molecular Dynamics.

Biomolecules 2019 03 23;9(3). Epub 2019 Mar 23.

Aurel Vlaicu University of Arad, Str. Elena Drăgoi 2-4, RO-310330 Arad, Romania.

Tau is a microtubule-associated protein that promotes microtubule assembly and stability. This protein is implicated in several neurodegenerative diseases, including Alzheimer's. To date, the three-dimensional (3D) structure of tau has not been fully solved, experimentally. Read More

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http://dx.doi.org/10.3390/biom9030116DOI Listing

New Approaches for the Discovery of Pharmacologically-Active Natural Compounds.

Biomolecules 2019 03 23;9(3). Epub 2019 Mar 23.

Department of Pharmacy, National Autonomous University of Mexico, Mexico City 04510, Mexico.

Natural products continue to be a major source of active compounds [... Read More

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http://dx.doi.org/10.3390/biom9030115DOI Listing

Spontaneous Switching among Conformational Ensembles in Intrinsically Disordered Proteins.

Biomolecules 2019 03 22;9(3). Epub 2019 Mar 22.

Department of Physics, North Carolina State University, Raleigh, NC, 27695, USA.

The common conception of intrinsically disordered proteins (IDPs) is that they stochastically sample all possible configurations driven by thermal fluctuations. This is certainly true for many IDPs, which behave as swollen random coils that can be described using polymer models developed for homopolymers. However, the variability in interaction energy between different amino acid sequences provides the possibility that some configurations may be strongly preferred while others are forbidden. Read More

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http://dx.doi.org/10.3390/biom9030114DOI Listing

Metformin Induces Different Responses in Clear Cell Renal Cell Carcinoma Caki Cell Lines.

Biomolecules 2019 03 22;9(3). Epub 2019 Mar 22.

Department of Pharmaceutical and Administrative Sciences, College of Pharmacy and Health Sciences, Drake University, Des Moines, IA 50311, USA.

Clear cell renal cell carcinoma (ccRCC) is the most common and lethal form of urological cancer diagnosed globally. Mutations of the von Hippel-Lindau tumor-suppressor gene and the resultant overexpression of hypoxia-inducible factor (HIF)-1α protein are considered hallmarks of ccRCC. Persistently activated HIF-1α is associated with increased cell proliferation, angiogenesis, and epithelial⁻mesenchymal transition (EMT), consequently leading to ccRCC progression and metastasis to other organs. Read More

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http://dx.doi.org/10.3390/biom9030113DOI Listing
March 2019
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Influence of Monoterpenes in Biological Activities of (Spreng.) Mez Essential Oils.

Biomolecules 2019 03 21;9(3). Epub 2019 Mar 21.

Pharmaceutical Sciences Graduate Program, Federal University of Rio Grande do Sul, Av. Ipiranga, 2752, 90610 000 Porto Alegre, Brazil.

Investigating the influence of seasonal variations on biological activities is important for pharmacological studies and metabolic engineering. Therefore, this study was conducted to determine the variation of the chemical composition of essential oils obtained from leaves, collected at different stages of plant development, as well as its influence on the biological activities. A total of 38 compounds were identified that accounted for 97⁻99. Read More

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http://dx.doi.org/10.3390/biom9030112DOI Listing

Human Semen Samples with High Antioxidant Reservoir May Exhibit Lower Post-Cryopreservation Recovery of Sperm Motility.

Biomolecules 2019 03 19;9(3). Epub 2019 Mar 19.

Department of Medical Laboratory Sciences, Jordan University of Science and Technology, Irbid 22110, Jordan.

Cryopreservation-thawing of human semen was found to reduce the level of antioxidant activity surrounding the sperm, which may negatively affect post-cryopreservation (post-thaw) recovery of sperm motility. Therefore, the current manufactured cryoprotectant media have been supplemented with certain antioxidants to preserve the loss in seminal antioxidant activity. In this study, we aimed to explore the correlation between total antioxidant capacity (TAC) of human semen samples before cryopreservation and the post-thaw recovery of sperm motility. Read More

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http://dx.doi.org/10.3390/biom9030111DOI Listing

Effect of Antifreeze Glycoproteins on Organoid Survival during and after Hypothermic Storage.

Biomolecules 2019 03 19;9(3). Epub 2019 Mar 19.

Max Planck Institute for Polymer Research, Ackermanweg 10, D-55128 Mainz, Germany.

We study the effect of antifreeze glycoproteins (AFGPs) on the survival of organoids under hypothermic conditions. We find that the survival of organoids in cold conditions depends on their developmental stage. Mature organoids die within 24 h when being stored at 4 °C, while cystic organoids can survive up to 48 h. Read More

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http://dx.doi.org/10.3390/biom9030110DOI Listing

Antimicrobial Films Based on Nanocomposites of Chitosan/Poly(vinyl alcohol)/Graphene Oxide for Biomedical Applications.

Biomolecules 2019 03 18;9(3). Epub 2019 Mar 18.

Programa de Química, Facultad de Ciencias, Universidad del Atlántico, Carrera 30 No. 8-49, Puerto Colombia 081008, Colombia.

Today, tissue regeneration is one of the greatest challenges in the field of medicine, since it represents hope after accidents or illnesses. Tissue engineering is the science based on improving or restoring tissues and organs. In this work, five formulations of chitosan/poly(vinyl alcohol)/graphene oxide (CS/PVA/GO) nanocomposites were studied for the development of biodegradable films with potential biomedical applications. Read More

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http://dx.doi.org/10.3390/biom9030109DOI Listing

Diagnostic Value of Soluble Urokinase-Type Plasminogen Activator Receptor in Addition to High-Sensitivity Troponin I in Early Diagnosis of Acute Myocardial Infarction.

Biomolecules 2019 03 18;9(3). Epub 2019 Mar 18.

Department of General and Interventional Cardiology, University Heart Center, 20246 Hamburg, Germany.

The soluble urokinase-type plasminogen activator receptor (suPAR) is a new marker for immune activation and inflammation and may provide diagnostic value on top of established biomarkers in patients with suspected acute myocardial infarction (AMI). Here, we evaluate the diagnostic potential of suPAR levels on top of high-sensitivity troponin I (hs-TnI) in a cohort of patients with suspected AMI. A total of 1220 patients presenting to the emergency department with suspected AMI were included, of whom 245 were diagnosed with AMI. Read More

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http://dx.doi.org/10.3390/biom9030108DOI Listing
March 2019
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Association of MMP9-1562C/T and MMP13-77A/G Polymorphisms with Non-Small Cell Lung Cancer in Southern Chinese Population.

Biomolecules 2019 03 18;9(3). Epub 2019 Mar 18.

Key Laboratory of Biological Nanomaterials and Devices, College of Life Sciences and Chemistry, Hunan University of Technology, Zhuzhou 412007, China.

Background: Matrix metalloproteinases (MMPs) are capable of degrading and modifying most components of the extracellular matrix (ECM) and the basal membrane (BM), and play crucial roles in cancer invasion and metastasis. MMP gene expressions were regulated primarily at the transcriptional level, which was associated with tumor spread and patient prognosis. Polymorphisms in MMPs have been reported to be associated with non-small cell lung cancer (NSCLC). Read More

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http://dx.doi.org/10.3390/biom9030107DOI Listing
March 2019
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Plant-Derived Bioactives in Oral Mucosal Lesions: A Key Emphasis to Curcumin, Lycopene, Chamomile, , Green Tea and Coffee Properties.

Biomolecules 2019 03 17;9(3). Epub 2019 Mar 17.

Food Safety Research Center (Salt), Semnan University of Medical Sciences, Semnan 35198-99951, Iran.

Oral mucosal lesions have many etiologies, including viral or bacterial infections, local trauma or irritation, systemic disorders, and even excessive alcohol and tobacco consumption. Folk knowledge on medicinal plants and phytochemicals in the treatment of oral mucosal lesions has gained special attention among the scientific community. Thus, this review aims to provide a brief overview on the traditional knowledge of plants in the treatment of oral mucosal lesions. Read More

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http://dx.doi.org/10.3390/biom9030106DOI Listing

Structure and Functions of Microtubule Associated Proteins Tau and MAP2c: Similarities and Differences.

Biomolecules 2019 03 16;9(3). Epub 2019 Mar 16.

Central European Institute of Technology, Masaryk University, Kamenice 5, 625 00 Brno, Czech Republic.

The stability and dynamics of cytoskeleton in brain nerve cells are regulated by microtubule associated proteins (MAPs), tau and MAP2. Both proteins are intrinsically disordered and involved in multiple molecular interactions important for normal physiology and pathology of chronic neurodegenerative diseases. Nuclear magnetic resonance and cryo-electron microscopy recently revealed propensities of MAPs to form transient local structures and long-range contacts in the free state, and conformations adopted in complexes with microtubules and filamentous actin, as well as in pathological aggregates. Read More

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http://dx.doi.org/10.3390/biom9030105DOI Listing

Class II PI3Ks at the Intersection between Signal Transduction and Membrane Trafficking.

Biomolecules 2019 03 15;9(3). Epub 2019 Mar 15.

Department of Molecular Biotechnology and Health Sciences, Molecular Biotechnology Center, University of Turin, 10126 Turin, Italy.

Phosphorylation of inositol phospholipids by the family of phosphoinositide 3-kinases (PI3Ks) is crucial in controlling membrane lipid composition and regulating a wide range of intracellular processes, which include signal transduction and vesicular trafficking. In spite of the extensive knowledge on class I PI3Ks, recent advances in the study of the three class II PI3Ks (PIK3C2A, PIK3C2B and PIK3C2G) reveal their distinct and non-overlapping cellular roles and localizations. By finely tuning membrane lipid composition in time and space among different cellular compartments, this class of enzymes controls many cellular processes, such as proliferation, survival and migration. Read More

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http://dx.doi.org/10.3390/biom9030104DOI Listing

Diagnostic and Prognostic Significance of Carboxypeptidase A4 (CPA4) in Breast Cancer.

Biomolecules 2019 03 14;9(3). Epub 2019 Mar 14.

Department of Surgery, Institute of Oncology, Istanbul University, 34093 Istanbul, Turkey.

Recent research focused on prolonged survival has suggested that carboxypeptidase A4 (CPA4) plays a role in both tumor microenvironment formation and distant metastasis in cancer. In some patients, serum and expression (mRNA) levels of CPA4 have been found to be correlated with the aggressiveness and progression of the disease. Accordingly, we conducted a first study to investigate the diagnostic and prognostic significance of CPA4 in the case of breast cancer (BC), the most common form of malignancy in women. Read More

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http://dx.doi.org/10.3390/biom9030103DOI Listing

Analysis of Flavonoids Bioactivity for Cholestatic Liver Disease: Systematic Literature Search and Experimental Approaches.

Biomolecules 2019 03 14;9(3). Epub 2019 Mar 14.

Unidad de Biomedicina, Facultad de Estudios Superiores-Iztacala, Universidad Nacional Autónoma de México, Tlalnepantla 54090, Mexico.

Flavonoids are naturally occurring compounds that show health benefits on the liver. However, there is little investigation about identification and evaluation of new flavonoid-containing drugs for cholestatic liver disease, one of the most common liver illnesses. We aimed to a systematic search regarding efficacy of flavonoids for treatment of cholestatic liver disease, and then evaluate naringenin (NG) as representative flavonoid in an obstructive cholestasis model. Read More

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http://dx.doi.org/10.3390/biom9030102DOI Listing

Cellular and Immunohistochemical Changes in Anaphylactic Shock Induced in the Ovalbumin-Sensitized Wistar Rat Model.

Biomolecules 2019 03 13;9(3). Epub 2019 Mar 13.

Department of Emergency Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA.

Anaphylactic shock (AS) is a life-threatening, multisystem disorder arising from sudden release of mast cell- and basophil-derived mediators into the circulation. In this study, we have used a Wistar rat model to investigate AS-associated histopathologic changes in various organs. Rats were sensitized with ovalbumin (1 mg s. Read More

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http://dx.doi.org/10.3390/biom9030101DOI Listing
March 2019
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Omega-3 Fatty Acids Prevent Post-Traumatic Stress Disorder-Induced Memory Impairment.

Biomolecules 2019 03 12;9(3). Epub 2019 Mar 12.

Department of Clinical Pharmacy, Jordan University of Science and Technology, Irbid 22110, Jordan.

Post-traumatic stress disorder (PTSD) is a psychiatric disorder that can happen after exposure to a traumatic event. Post-traumatic stress disorder is common among mental health disorders that include mood and anxiety disorders. Omega-3 fatty acids (OMGs) are essential for the maintenance of brain function and prevention of cognition dysfunctions. Read More

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http://dx.doi.org/10.3390/biom9030100DOI Listing
March 2019
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Antidiabetic Properties of Naringenin: A Citrus Fruit Polyphenol.

Biomolecules 2019 03 12;9(3). Epub 2019 Mar 12.

Department of Health Sciences, Brock University, St. Catharines, ON L2S 3A1, Canada.

Type 2 diabetes mellitus (T2DM) is a metabolic disease characterized by insulin resistance and hyperglycemia and is associated with personal health and global economic burdens. Current strategies/approaches of insulin resistance and T2DM prevention and treatment are lacking in efficacy resulting in the need for new preventative and targeted therapies. In recent years, epidemiological studies have suggested that diets rich in vegetables and fruits are associated with health benefits including protection against insulin resistance and T2DM. Read More

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http://dx.doi.org/10.3390/biom9030099DOI Listing
March 2019
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Structural Analysis of the 42 kDa Parvulin of .

Biomolecules 2019 03 7;9(3). Epub 2019 Mar 7.

University Duisburg-Essen, Research Group Structural and Medicinal Biochemistry, Centre for Medical Biotechnology (ZMB), University of Duisburg-Essen, 45117 Essen, Germany.

is a unicellular eukaryotic parasite, which causes the African sleeping sickness in humans. The recently discovered trypanosomal protein Parvulin 42 (Par42) plays a key role in parasite cell proliferation. Homologues of this two-domain protein are exclusively found in protozoa species. Read More

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http://dx.doi.org/10.3390/biom9030093DOI Listing
March 2019
3 Reads