1,435 results match your criteria Biomicrofluidics[Journal]


A novel wide-range microfluidic dilution device for drug screening.

Biomicrofluidics 2019 Mar 22;13(2):024105. Epub 2019 Mar 22.

National Key Laboratory of Science and Technology on Micro/Nano Fabrication, Department of Micro/Nano Electronics, School of Electronic Information and Electrical Engineering, Shanghai Jiao Tong University, Shanghai 200240, People's Republic of China.

Microfluidic dilution chip is a crucial approach to perform gradient dilution of experimental samples in many biological investigations. In this study, we developed two serial wide-range dilution chips with dilution rates of 1:1 and 1:4 on the basis of the microfluidic oscillator by designing a series chamber, which was similar to a series circuit. The size of this chamber was adjusted and mixed with the neighboring air chamber to form dilution rates by oscillatory methods. Read More

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http://dx.doi.org/10.1063/1.5085865DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6430636PMC
March 2019
1 Read

Detachable glass micro/nanofluidic device.

Biomicrofluidics 2019 Mar 14;13(2):024104. Epub 2019 Mar 14.

Department of Applied Chemistry, Graduate School of Engineering, The University of Tokyo, Tokyo 113-8656, Japan.

Glass is one of the most ideal materials for micro/nanofluidic devices due to its excellent optical transparency, resistance to a wide range of solvents and reagents, and easy to modify surfaces by silane-coupling reagents. From a practical point of view, glass is a hard material and is suitable for real applications. One of the advantages of glass is its reusability; however, this reusability is difficult to realize in certain conditions. Read More

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http://dx.doi.org/10.1063/1.5087003DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6417905PMC

Boundary waves in a microfluidic device as a model for intramural periarterial drainage.

Biomicrofluidics 2019 Mar 8;13(2):024103. Epub 2019 Mar 8.

Department of Mechanical Engineering, State University of New York at Binghamton, Binghamton, New York 13902, USA.

The failure to clear amyloid-Beta from an aging brain leads to its accumulation within the walls of arteries and potentially to Alzheimer's disease. However, the clearance mechanism through the intramural periarterial pathway is not well understood. We previously proposed a hydrodynamic reverse transport model for the cerebral arterial basement membrane pathway. Read More

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http://dx.doi.org/10.1063/1.5080446DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6408319PMC

Analytical study of AC electroosmotic mixing in 2-dimensional microchannel with time periodic surface potential.

Biomicrofluidics 2019 Mar 8;13(2):024102. Epub 2019 Mar 8.

Department of Chemical Engineering, Pohang University of Science and Technology, Pohang 37673, South Korea.

This work reported an analytic study of AC electroosmotic flows with a view to control the degree of mixing in a rectangular microchannel. Only with spatially non-uniform zeta potential distribution, fluid particles travel back and forth along a vortical flow field developed inside a microchannel. Although complex patterns of electroosmotic vortical flows can be obtained by various types of non-uniform zeta potential distributions, fluid particles always follow regular paths due to a laminar flow limit. Read More

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http://dx.doi.org/10.1063/1.5091936DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6408320PMC

Orthogonal co-cultivation of smooth muscle cell and endothelial cell layers to construct -like vasculature.

Biomicrofluidics 2019 Jan 26;13(1):014115. Epub 2019 Feb 26.

Department of Chemical Engineering, College of Engineering, Kyung Hee University, 1 Seochon-dong, Giheung-gu, Yongin-si, Gyeonggi-do 17104, South Korea.

Development of a three-dimensional (3D) vascular co-cultivation system is one of the major challenges to provide an advanced analytical platform for studying blood vessel related diseases. To date, however, the -like vessel system has not been fully realized due to the difficulty of co-cultivation of the cells with orthogonal alignment. In this study, we report the utilization of microfabrication technology to construct biomimetic 3D co-cultured vasculature. Read More

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http://aip.scitation.org/doi/10.1063/1.5068689
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http://dx.doi.org/10.1063/1.5068689DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6404948PMC
January 2019
5 Reads

An automated microfluidic system for selection of aptamer probes against ovarian cancer tissues.

Biomicrofluidics 2019 Jan 25;13(1):014114. Epub 2019 Feb 25.

Department of Obstetrics and Gynecology, National Cheng Kung University Hospital, College of Medicine, Tainan 70403, Taiwan.

Because of the difficulty of treatment in its latest stages, cancer is among the leading causes of death worldwide. Therefore, high-affinity and specificity biomarkers are still in demand for many cancer types, and the utility of aptamers to serve in this regard has been explored recently. Although a process known as "systematic evolution of ligands by exponential enrichment" (SELEX) has been used to generate aptamer-based cancer biomarkers, this approach is complicated, time-consuming, and labor-intensive. Read More

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http://dx.doi.org/10.1063/1.5085133DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6404914PMC
January 2019
2 Reads

Joule heating-induced particle manipulation on a microfluidic chip.

Biomicrofluidics 2019 Jan 22;13(1):014113. Epub 2019 Feb 22.

Department of Mechanical Engineering, Indian Institute of Technology Kharagpur, Kharagpur, West Bengal 721302, India.

We develop an electrokinetic technique that continuously manipulates colloidal particles to concentrate into patterned particulate groups in an energy efficient way, by exclusive harnessing of the intrinsic Joule heating effects. Our technique exploits the alternating current electrothermal flow phenomenon which is generated due to the interaction between non-uniform electric and thermal fields. Highly non-uniform electric field generates sharp temperature gradients by generating spatially-varying Joule heat that varies along the radial direction from a concentrated point hotspot. Read More

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http://dx.doi.org/10.1063/1.5082978DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6404938PMC
January 2019

Generation of functional hepatocyte 3D discoids in an acoustofluidic bioreactor.

Biomicrofluidics 2019 Jan 12;13(1):014112. Epub 2019 Feb 12.

Mechanical Engineering, Faculty of Engineering and Physical Sciences, University of Southampton, Southampton SO17 1BJ, United Kingdom.

Ultrasonic standing wave systems have previously been used for the generation of 3D constructs for a range of cell types. In the present study, we cultured cells from the human hepatoma Huh7 cell line in a Bulk Acoustic Wave field and studied their viability, their functions, and their response to the anti-cancer drug, 5 Fluorouracil (5FU). We found that cells grown in the acoustofluidic bioreactor (AFB) expressed no reduction in viability up to 6 h of exposure compared to those cultured in a conventional 2D system. Read More

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http://dx.doi.org/10.1063/1.5082603DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6404912PMC
January 2019

Microfluidic rectifier for polymer solutions flowing through porous media.

Biomicrofluidics 2019 Jan 11;13(1):014111. Epub 2019 Feb 11.

Department of Chemical Engineering, Delft University of Technology, Van der Maasweg 9, 2629 HZ Delft, The Netherlands.

Fluidic rectification refers to anisotropic flow resistance upon changing the flow direction. Polymeric solutions, in contrast to Newtonian fluids, can exhibit an anisotropic flow resistance in microfluidic devices by tuning the channel shape at low Reynolds number. Such a concept has not been investigated in an anisotropic porous medium. Read More

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http://dx.doi.org/10.1063/1.5050201DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6404928PMC
January 2019

Development of a microfluidic platform for size-based hydrodynamic enrichment and PSMA-targeted immunomagnetic isolation of circulating tumour cells in prostate cancer.

Biomicrofluidics 2019 Jan 8;13(1):014110. Epub 2019 Feb 8.

Faculty of Health Sciences, Simon Fraser University, 8888 University Drive, Burnaby, British Columbia V5A 1S6, Canada.

Efforts to further improve the clinical management of prostate cancer (PCa) are hindered by delays in diagnosis of tumours and treatment deficiencies, as well as inaccurate prognoses that lead to unnecessary or inefficient treatments. The quantitative and qualitative analysis of circulating tumour cells (CTCs) may address these issues and could facilitate the selection of effective treatment courses and the discovery of new therapeutic targets. Therefore, there is much interest in isolation of elusive CTCs from blood. Read More

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http://dx.doi.org/10.1063/1.5064473DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6404957PMC
January 2019

Rheological behavior of bimodal distribution emulsions on flow adoptability.

Biomicrofluidics 2019 Jan 7;13(1):014109. Epub 2019 Feb 7.

Department of Mechanical Engineering, Yonsei University, Seoul 03772, South Korea.

This paper analyzed colloidal characteristics of a bimodal distribution emulsion system using bulk rheological and numerical approaches. The experiment measured simple shear to confirm emulsion shear thinning and viscosity tendencies. Numerical models employed the multi-component lattice Boltzmann method to express interfacial tension, surfactant movement, and viscosity of liquid phases. Read More

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http://dx.doi.org/10.1063/1.5083858DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6404935PMC
January 2019

Microfluidic-based observation of local bacterial density under antimicrobial concentration gradient for rapid antibiotic susceptibility testing.

Biomicrofluidics 2019 Jan 5;13(1):014108. Epub 2019 Feb 5.

Graduate School of Nanoscience and Technology, Korea Advanced Institute of Science and Technology, Daejeon 34141, South Korea.

The need for accurate and efficient antibiotic susceptibility testing (AST) has been emphasized with respect to the emerging antimicrobial resistance of pathogenic bacteria which has increased over the recent decades. In this study, we introduce a microfluidic system that enables rapid formation of the antibiotic concentration gradient with convenient bacterial growth measurement based on color scales. Furthermore, we expanded the developed system to analyze combinatory effects of antibiotics and measured the collective antibiotic susceptibility of bacteria compared to single microfluidic AST methods. Read More

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http://dx.doi.org/10.1063/1.5066558DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6404913PMC
January 2019
4 Reads

A human proximal tubule-on-a-chip to study renal disease and toxicity.

Biomicrofluidics 2019 Jan 1;13(1):014107. Epub 2019 Feb 1.

Department of Biomedical Engineering, Binghamton University, Binghamton, New York 13902, USA.

Renal disease is a global problem with unsustainable health-care costs. There currently exists a lack of accurate human renal disease models that take into account the complex microenvironment of these tissues. Here, we present a reusable microfluidic model of the human proximal tubule and glomerulus, which allows for the growth of renal epithelial cells in a variety of conditions that are representative of renal disease states including altered glomerular filtration rate, hyperglycemia, nephrolithiasis, and drug-induced nephrotoxicity (cisplatin and cyclosporine). Read More

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http://dx.doi.org/10.1063/1.5083138DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6404920PMC
January 2019
3 Reads

A microfluidic device for fixation and super-resolved mechanosensation studies of primary cilia.

Biomicrofluidics 2019 Jan 25;13(1):014105. Epub 2019 Jan 25.

Institute of Atomic and Molecular Sciences, Academia Sinica, Taipei 10617, Taiwan.

The primary cilium plays an important role in mechanosensation in mammalian cells. To understand mechanosensation in the primary cilium, we combined a microfluidic device with super-resolution microscopy to study the primary cilium phenotypes. The microfluidic system enabled the precise control of the flow shear within a well-confined cell-culture environment. Read More

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http://dx.doi.org/10.1063/1.5081756DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6404955PMC
January 2019
1 Read

3D printed microfluidic viscometer based on the co-flowing stream.

Biomicrofluidics 2019 Jan 8;13(1):014104. Epub 2019 Jan 8.

School of Mechanical Engineering, Pusan National University, Busan 46241, South Korea.

Considering the role of viscosity in the dynamics of physical, chemical, and biological systems, accurate measurement of viscosity is essential. Although many conventional viscometers have been widely used, these conventional viscometers suffer from some drawbacks. In this study, a three-dimensional (3D) printed microfluidic viscometer was proposed based on the estimation of the pressure between two fluids to easily measure viscosity with small samples. Read More

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http://aip.scitation.org/doi/10.1063/1.5063425
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http://dx.doi.org/10.1063/1.5063425DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6404922PMC
January 2019
6 Reads

Biomimetic pulsatile flows through flexible microfluidic conduits.

Biomicrofluidics 2019 Jan 8;13(1):014103. Epub 2019 Jan 8.

Advanced Technology Development Center, Indian Institute of Technology Kharagpur, Kharagpur 721302, India.

We bring out unique aspects of the pulsatile flow of a blood analog fluid (Xanthan gum solution) in a biomimetic microfluidic channel. Pressure waveforms that mimic biologically consistent pulsations are applied on physiologically relevant cylindrical microchannels fabricated using polydimethylsiloxane. The features of the relevant waveforms like peak amplitude and dicrotic notch are reproduced . Read More

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http://aip.scitation.org/doi/10.1063/1.5065901
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http://dx.doi.org/10.1063/1.5065901DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6404934PMC
January 2019
4 Reads

Probing tumor microtissue formation and epithelial-mesenchymal transition on a well-mesh microchip.

Biomicrofluidics 2019 Jan 4;13(1):014102. Epub 2019 Jan 4.

Materials Genome Institute, Shanghai University, Shanghai 200444, China.

Three-dimensional cultures of tumor microtissues and biomimetic simulation of tumor microenvironments are of great significance in the study of tumorigenesis and development processes. In this study, a well-mesh microchip was developed to realize the formation and culture of tumor microtissues . Human lung adenocarcinoma HCC827 cells and large-cell lung cancer NCI-H460 cells were used. Read More

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http://dx.doi.org/10.1063/1.5064838DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6404933PMC
January 2019
1 Read

Reducing deposition of encrustation in ureteric stents by changing the stent architecture: A microfluidic-based investigation.

Biomicrofluidics 2019 Jan 4;13(1):014101. Epub 2019 Jan 4.

Department of Environment, Land and Infrastructure Engineering, Politecnico di Torino, Turin 10129, Italy.

Ureteric stents are clinically deployed to retain ureteral patency in the presence of an obstruction of the ureter lumen. Despite the fact that multiple stent designs have been researched in recent years, encrustation and biofilm-associated infections remain significant complications of ureteral stenting, potentially leading to the functional failure of the stent. It has been suggested that "inactive" side-holes of stents may act as anchoring sites for encrusting crystals, as they are associated with low wall shear stress (WSS) levels. Read More

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http://dx.doi.org/10.1063/1.5059370DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6404931PMC
January 2019
1 Read

Solid-state nanopore hydrodynamics and transport.

Biomicrofluidics 2019 Jan 30;13(1):011301. Epub 2019 Jan 30.

Department of Chemical and Biomolecular Engineering, University of Notre Dame, Notre Dame, Indiana 46556, USA.

The resistive pulse method based on measuring the ion current trace as a biomolecule passing through a nanopore has become an important tool in biotechnology for characterizing molecules. A detailed physical understanding of the translocation process is essential if one is to extract the relevant molecular properties from the current signal. In this Perspective, we review some recent progress in our understanding of hydrodynamic flow and transport through nanometer sized pores. Read More

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http://dx.doi.org/10.1063/1.5083913DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6404949PMC
January 2019

Engaging the microfluidics and nanofluidics community into the next decade.

Authors:
Leslie Y Yeo

Biomicrofluidics 2019 Jan 24;13(1):010401. Epub 2019 Jan 24.

RMIT University, Melbourne, VIC 3000, Australia.

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http://dx.doi.org/10.1063/1.5088575DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6404940PMC
January 2019

Erratum: Publisher's Note: 'Low temperature flow lithography' [Biomicrofluidics 12, 054105 (2018)].

Biomicrofluidics 2018 Nov 21;12(6):069901. Epub 2018 Dec 21.

Chemical and Biological Engineering, Korea University, Seoul 02841, Republic of Korea.

[This corrects the article DOI: 10.1063/1.5047016. Read More

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http://dx.doi.org/10.1063/1.5084274DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6407851PMC
November 2018

Overlimiting current due to electro-diffusive amplification of the second Wien effect at a cation-anion bipolar membrane junction.

Biomicrofluidics 2018 Nov 28;12(6):064107. Epub 2018 Dec 28.

Laboratory of Electro-Hydrodynamics of Micro- and Nanoscales, Department of Mathematics and Computer Science, Financial University, Krasnodar 350051, Russian Federation.

Numerical simulations are presented for the transient and steady-state response of a model electrodiffusive cell with a bipolar ion-selective membrane under electric current. The model uses a continuum Poisson-Nernst-Planck theory including source terms to account for the catalytic second Wien effect between ionogenic groups in the membranes and resolves the Debye layers at interfaces. The resulting electric field at the membrane junction is increased by as much as four orders of magnitude in comparison to the field external to the membrane. Read More

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http://dx.doi.org/10.1063/1.5066195DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6404929PMC
November 2018
1 Read

Two-way detection of image features and immunolabeling of lymphoma cells with one-step microarray analysis.

Biomicrofluidics 2018 Nov 28;12(6):064106. Epub 2018 Dec 28.

School of Medical Laboratory, Tianjin Medical University, Tianjin 300203, People's Republic of China.

Detecting the number of pathological lymphoma cells and lymphocyte subtypes in blood is helpful for clinical diagnosis and typing of lymphoma. In the current study, cell type is identified by cell morphological features and immunolabeled lymphocyte subtypes. Red blood cells and leukocytes were separated using a microfluidic cell chip based on physical blood cell parameters, and leukocytes were identified using five characteristic parameters: energy variance, entropy variance, moment of inertia variance, color mean, and cell area individually. Read More

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http://aip.scitation.org/doi/10.1063/1.5063369
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http://dx.doi.org/10.1063/1.5063369DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6404911PMC
November 2018
2 Reads

Rapid prototyping of fluoropolymer microchannels by xurography for improved solvent resistance.

Biomicrofluidics 2018 Nov 14;12(6):064105. Epub 2018 Dec 14.

Department of Applied Mechanics, Waseda University, 3-4-1 Okubo, Shinjuku-ku, Tokyo 169-8555, Japan.

Microchannels made of fluoropolymers show potential merits due to their excellent solvent resistance, but such channels have not been widely used because of the complexity to fabricate them. This communication describes a method to prototype microfluidic devices using fluoropolymer films. The fabrication requires only two steps; cutting fluoropolymer films with a desktop cutting plotter and applying heat and pressure to laminate them. Read More

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http://dx.doi.org/10.1063/1.5051666DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6404952PMC
November 2018

Microstencil-based spatial immobilization of individual cells for single cell analysis.

Biomicrofluidics 2018 Nov 28;12(6):064104. Epub 2018 Nov 28.

Department of Bioengineering, George Mason University, Fairfax, Virginia 22033, USA.

Cells exhibit biologically heterogeneous phenotypes, particularly in pathogenic states. To study cell behavior at the single cell level, a variety of micropatterning techniques have been proposed that allow the spatial organization of cells with great control over cell volume, morphology, and intercellular interactions. Among these strategies, microstencil patterning has traditionally been eschewed due to fragility of membranes and lack of control over cell configurations within patterns. Read More

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http://dx.doi.org/10.1063/1.5061922DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6404921PMC
November 2018

Editorial: Farewell from the Founding and Chief Editor of .

Authors:
Hsueh-Chia Chang

Biomicrofluidics 2018 Nov 14;12(6):060401. Epub 2018 Dec 14.

University of Notre Dame, Notre Dame, Indiana 46556, USA.

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http://dx.doi.org/10.1063/1.5084549DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6404945PMC
November 2018

Impact of poloxamer 188 (Pluronic F-68) additive on cell mechanical properties, quantification by real-time deformability cytometry.

Biomicrofluidics 2018 Jul 22;12(4):044118. Epub 2018 Aug 22.

Institute of Biological Chemistry, Biophysics and Bioengineering, School of Engineering and Physical Science, Heriot-Watt University, Edinburgh Campus, Edinburgh EH14 4AS, United Kingdom.

Advances in cellular therapies have led to the development of new approaches for cell product purification and formulation, e.g., utilizing cell endogenous properties such as size and deformability as a basis for separation from potentially harmful undesirable by-products. Read More

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http://dx.doi.org/10.1063/1.5040316DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6404947PMC

High-throughput culture and embedment of spheroid array using droplet contact-based spheroid transfer.

Biomicrofluidics 2018 Jul 18;12(4):044109. Epub 2018 Jul 18.

Department of Bio and Brain Engineering, Korea Advanced Institute of Science and Technology (KAIST), 291 Daehak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea.

Spheroids are one of the most representative models of 3D cell culture, which can be easily formed using conventional hanging drop method. However, medium change and spheroid transferring process are the bottlenecks that reduce the throughput of the entire process in the hanging drop culture. In addition, the embedment of spheroid into hydrogel still depends on the individual pipetting process. Read More

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http://dx.doi.org/10.1063/1.5039965DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6404923PMC

An artificial placenta type microfluidic blood oxygenator with double-sided gas transfer microchannels and its integration as a neonatal lung assist device.

Biomicrofluidics 2018 Jul 3;12(4):044101. Epub 2018 Jul 3.

Department of Pediatrics, McMaster University, Hamilton, Ontario L8S 4L7, Canada.

Preterm neonates suffering from respiratory distress syndrome require assistive support in the form of mechanical ventilation or extracorporeal membrane oxygenation, which may lead to long-term complications or even death. Here, we describe a high performance artificial placenta type microfluidic oxygenator, termed as a double-sided single oxygenator unit (dsSOU), which combines microwire stainless-steel mesh reinforced gas permeable membranes on both sides of a microchannel network, thereby significantly reducing the diffusional resistance to oxygen uptake as compared to the previous single-sided oxygenator designs. The new oxygenator is designed to be operated in a pumpless manner, perfused solely due to the arterio-venous pressure difference in a neonate and oxygenate blood through exposure directly to ambient atmosphere without any air or oxygen pumping. Read More

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http://dx.doi.org/10.1063/1.5034791DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6404930PMC
July 2018
3 Reads

Thermal sensing in fluid at the micro-nano-scales.

Biomicrofluidics 2018 Jul 2;12(4):041501. Epub 2018 Jul 2.

Key Laboratory for the Physics and Chemistry of Nanodevices, Department of Electronics, Peking University, Beijing 100871, People's Republic of China.

Temperature is one of the most fundamental parameters for the characterization of a physical system. With rapid development of lab-on-a-chip and biology at single cell level, a great demand has risen for the temperature sensors with high spatial, temporal, and thermal resolution. Nevertheless, measuring temperature in liquid environment is always a technical challenge. Read More

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http://aip.scitation.org/doi/10.1063/1.5037421
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http://dx.doi.org/10.1063/1.5037421DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6404956PMC
July 2018
5 Reads

Reconfiguring droplet interface bilayer networks through sacrificial membranes.

Biomicrofluidics 2018 May 12;12(3):034112. Epub 2018 Jun 12.

School of Environmental, Civil, Agricultural, and Mechanical Engineering, College of Engineering, University of Georgia, Athens, Georgia 30602, USA.

The droplet interface bilayer platform allows for the fabrication of stimuli-responsive microfluidic materials, using phospholipids as an organic surfactant in water-in-oil mixtures. In this approach, lipid-coated droplets are adhered together in arranged networks, forming lipid bilayer membranes with embedded transporters and establishing selective exchange pathways between neighboring aqueous subcompartments. The resulting material is a biologically inspired droplet-based material that exhibits emergent properties wherein different droplets accomplish different functions, similar to multicellular organisms. Read More

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http://dx.doi.org/10.1063/1.5023386DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6404924PMC

Membrane isolation of repeated-use sweat stimulants for mitigating both direct dermal contact and sweat dilution.

Biomicrofluidics 2018 May 1;12(3):034101. Epub 2018 May 1.

Department of Electrical Engineering and Computer Science, University of Cincinnati, Cincinnati, Ohio 45221, USA.

With the device integration of sweat stimulation, sweat becomes a stronger candidate for non-invasive continuous biochemical sensing. However, sweat stimulants are cholinergenic agents and non-selective to just the sweat glands, and so, direct placement of sweat stimulants poses additional challenges in the possibility for uncontrollable transport of the stimulant into the body and challenges in contamination of the sweat sample. Reported here is membrane isolation of repeated-use sweat stimulants for mitigating direct dermal contact, dilution of the sweat stimulant, and contamination of the sweat sample. Read More

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http://dx.doi.org/10.1063/1.5023396DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6404941PMC
May 2018
1 Read

ELIPatch, a thumbnail-size patch with immunospot array for multiplexed protein detection from human skin surface.

Biomicrofluidics 2018 May 3;12(3):031101. Epub 2018 May 3.

QuantaMatrix, Inc., Seoul 03122, South Korea.

Proteins secreted by skin have great potential as biomarkers for interpreting skin conditions. However, inconvenience in handling and bulky size of existing methods are existing limitations. Here, we describe a thumb-nail sized patch with the array of microdisks which captures multiple proteins from the skin surface. Read More

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http://dx.doi.org/10.1063/1.5032170DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6404946PMC

Quantitative assessment of chemotropism in pollen tubes using microslit channel filters.

Biomicrofluidics 2018 Mar 30;12(2):024113. Epub 2018 Mar 30.

Division of Biological Science, Graduate School of Science, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Aichi 464-8602, Japan.

We present a semi- chemotropism assay that can be used to evaluate the chemoattractant effect of diffusible plant signaling molecules on growing pollen tubes. We constructed an array of microslit channels in a microfluidic device that prevented the passage of randomly growing pollen tubes but permitted ones that are responsive to the chemoattractant. Depending on the microslit channel size, 80%-100% of the randomly growing pollen tubes were excluded from reaching the source of the attractant. Read More

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http://dx.doi.org/10.1063/1.5023718DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6404937PMC

Hydrogel droplet single-cell processing: DNA purification, handling, release, and on-chip linearization.

Biomicrofluidics 2018 Mar 14;12(2):024107. Epub 2018 Mar 14.

Department of Physics, McGill University, 3600 Rue University, Montreal, Quebec H3A 2T8, Canada.

The preparation and handling of mammalian single-cell genomic DNA is limited by the complexity bottleneck inherent to performing multi-step, multi-reagent operations in a microfluidic environment. We have developed a method for benchtop preparation of high-molecular weight, intact, single-cell genomes and demonstrate the extraction of long nucleic acid molecules in a microfluidic system. Lymphoblasts are encapsulated inside of alginate microparticles using a droplet microfluidics, and cells are lysed in bulk. Read More

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http://dx.doi.org/10.1063/1.5020571DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6404942PMC
March 2018
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Label-free extraction of extracellular vesicles using centrifugal microfluidics.

Biomicrofluidics 2018 Mar 6;12(2):024103. Epub 2018 Mar 6.

Singapore Institute of Manufacturing Technology, ASTAR, Singapore 138634.

Extracellular vesicles (EVs) play an important role as active messengers in intercellular communication and distant microenvironment modeling. Increasingly, these EVs are recognized as important biomarkers for clinical diagnostics. However, current isolation methods of EVs are time-consuming and ineffective due to the high diffusive characteristics of nanoparticles coupled with fluid flow instability. Read More

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http://dx.doi.org/10.1063/1.5019983DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6404916PMC

A noninvasive light driven technique integrated microfluidics for zebrafish larvae transportation.

Biomicrofluidics 2018 Mar 27;12(2):021101. Epub 2018 Mar 27.

Department of Mechanical Engineering, National Cheng Kung University, Tainan 701, Taiwan.

Transferring the zebrafish larvae on an imaging platform has long been performed manually by the use of forceps or through mechanical pumping. These methods induce detrimental damages to the fragile bodies of zebrafish larvae during the transportation. To address this issue, in this work we are devising a light driven technique to transport zebrafish larvae within a microfluidic environment. Read More

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http://dx.doi.org/10.1063/1.5027014DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6404953PMC
March 2018
4 Reads

Geometrically enhanced sensor surfaces for the selective capture of cell-like particles in a laminar flow field.

Biomicrofluidics 2018 Jan 20;12(1):014116. Epub 2018 Feb 20.

Department of Microsystems Engineering, University of Freiburg, Freiburg im Breisgau, Baden-Württemberg 79110, Germany.

Medical wires inserted into the blood stream of patients offer an attractive perspective to capture rare cells such as circulating tumor cells . A major challenge in such systems is to achieve an efficient interaction of the desired cells with the sensing surface and avoid those cells that simply flow by the wire without any contact while floating in a laminar flow field at some small distance to the sensor surface. We describe a new strategy to increase the interaction of cells or cell-like particles to such wire-shaped sensor surfaces both from an experimental and a theoretical point of view. Read More

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http://dx.doi.org/10.1063/1.5017714DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6404926PMC
January 2018

3D printed selectable dilution mixer pumps.

Biomicrofluidics 2019 Jan 30;13(1):014106. Epub 2019 Jan 30.

Electrical and Computer Engineering Department, Brigham Young University, Provo, Utah 84602, USA.

In this paper, we demonstrate the ability to 3D print tightly integrated structures with active valves, pumps, and mixers, and we use our compact chip-to-chip interconnects [Gong , Lab Chip , 639-647 (2018)] to move bulky world-to-chip connections to separate interface chips for both post-print flushing and post-cure device operation. As example devices, we first examine 3D printed pumps, followed by two types of selectable ratio mixer pumps, a linear dilution mixer pump (LDMP) and a parallelized dilution mixer pump (PDMP), which occupy volumes of only and , respectively. The LDMP generates a selectable dilution ratio from a linear set of possibilities, while the PDMP generates a denser set of possible dilutions with a maximum dilution ratio of 1/16. Read More

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http://dx.doi.org/10.1063/1.5070068DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6353643PMC
January 2019

A nanofilter for fluidic devices by pillar-assisted self-assembly microparticles.

Biomicrofluidics 2018 Nov 19;12(6):064103. Epub 2018 Nov 19.

Department of Bioengineering, McGill University, Montreal, Quebec H3A 0E9, Canada.

We present a nanofilter based on pillar-assisted self-assembly microparticles for efficient capture of bacteria. Under an optimized condition, we simply fill the arrays of microscale pillars with submicron scale polystyrene particles to create a filter with nanoscale pore diameter in the range of 308 nm. The design parameters such as the pillar diameter and the inter-pillar spacing in the range of 5 m-40 m are optimized using a multi-physics finite element analysis and computational study based on bi-directionally coupled laminar flow and particle tracking solvers. Read More

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http://dx.doi.org/10.1063/1.5048623DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6242779PMC
November 2018
3 Reads

Paper-based immunosensor utilizing dielectrophoretic trapping of microprobes for quantitative and label free detection using electrochemical impedance spectroscopy.

Biomicrofluidics 2018 Nov 13;12(6):064102. Epub 2018 Nov 13.

Institute of Medical Science and Technology, National Sun Yat-sen University, Kaohsiung, Taiwan.

In this study, we have developed a novel paper based immunoassay for the quantitative detection of immunoreactions using electrochemical impedance spectroscopy. Paper provides an attractive platform for fabrication of simple, low cost, and portable diagnostic devices as it allows passive liquid transport, is biocompatible, and has tunable properties such as hydrophilicity, flexibility, permeability, and reactivity. We have used screen-printing to fabricate interdigitated electrodes (finger width and gap of 200 m) on the paper substrate, while UV-lithography enables patterning of the paper into hydrophobic/hydrophilic regions. Read More

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http://dx.doi.org/10.1063/1.5057731DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6234119PMC
November 2018
3 Reads
3.357 Impact Factor

measurements of hemodynamic forces and their effects on endothelial cell mechanics at the sub-cellular level.

Biomicrofluidics 2018 Nov 9;12(6):064101. Epub 2018 Nov 9.

Department of Mechanical & Materials Engineering, University of Nebraska-Lincoln, Lincoln, Nebraska 68588, USA.

This paper presents micro-particle tracking velocimetry measurements over cultured bovine aortic endothelial cell monolayers in microchannels. The objective was to quantify fluid forces and cell morphology at the sub-cellular scale for monolayers subjected to steady shear rates of 5, 10, and 20 dyn/cm. The ultimate goal of this study was to develop an experimental methodology for detailed study of physiologically realistic healthy and diseased conditions. Read More

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http://dx.doi.org/10.1063/1.5028122DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6226388PMC
November 2018

Shape-based separation of micro-/nanoparticles in liquid phases.

Biomicrofluidics 2018 Sep 23;12(5):051503. Epub 2018 Oct 23.

Chemical and Biochemical Engineering Department, Missouri University of Science and Technology, Rolla, Missouri 65409, USA.

The production of particles with shape-specific properties is reliant upon the separation of micro-/nanoparticles of particular shapes from particle mixtures of similar volumes. However, compared to a large number of size-based particle separation methods, shape-based separation methods have not been adequately explored. We review various up-to-date approaches to shape-based separation of rigid micro-/nanoparticles in liquid phases including size exclusion chromatography, field flow fractionation, deterministic lateral displacement, inertial focusing, electrophoresis, magnetophoresis, self-assembly precipitation, and centrifugation. Read More

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http://dx.doi.org/10.1063/1.5052171DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6207070PMC
September 2018
2 Reads

Biophysical analysis of fluid shear stress induced cellular deformation in a microfluidic device.

Biomicrofluidics 2018 Sep 17;12(5):054109. Epub 2018 Oct 17.

Cain Department of Chemical Engineering, Louisiana State University, Baton Rouge, Louisiana 70803, USA.

Even though the majority of breast cancers respond well to primary therapy, a large percentage of patients relapse with metastatic disease, for which there is no treatment. In metastasis, a tumor sheds a small number of cancerous cells, termed circulating tumor cells (CTCs), into the local vasculature, from where they spread throughout the body to form new tumors. As CTCs move through the circulatory system, they experience physiological forces not present in the initial tumor environment, namely, fluid shear stress (FSS). Read More

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http://aip.scitation.org/doi/10.1063/1.5063824
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http://dx.doi.org/10.1063/1.5063824DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6192794PMC
September 2018
12 Reads

Review Article: Capturing the physiological complexity of the brain's neuro-vascular unit .

Biomicrofluidics 2018 Sep 16;12(5):051502. Epub 2018 Oct 16.

Department of Mechanical Engineering, University of California, 6159 Etcheverry Hall, Berkeley, California 94720, USA.

With the accelerating pace of brain research in recent years and the growing appreciation of the complexity of the brain and several brain-associated neurological diseases, the demand for powerful tools to enhance drug screening, diagnosis, and fundamental research is greater than ever. Highly representative models of the central nervous system (CNS) can play a critical role in meeting these needs. Unfortunately, animal models lack controllability, are difficult to monitor, and do not model human-specific brain behavior accurately. Read More

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http://dx.doi.org/10.1063/1.5045126DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6191301PMC
September 2018
9 Reads

Microfluidic platforms for rapid screening of cancer affinity reagents by using tissue samples.

Biomicrofluidics 2018 Sep 3;12(5):054108. Epub 2018 Oct 3.

Department of Obstetrics and Gynecology, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan, Taiwan.

Cancer is the most serious disease worldwide, and ovarian cancer (OvCa) is the second most common type of gynecological cancer. There is consequently an urgent need for early-stage detection of OvCa, which requires affinity reagent biomarkers for OvCa. Systematic evolution of ligands by exponential enrichment (SELEX) and phage display technology are two powerful technologies for identifying affinity reagent biomarkers. Read More

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http://aip.scitation.org/doi/10.1063/1.5050451
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http://dx.doi.org/10.1063/1.5050451DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6170194PMC
September 2018
7 Reads

Miniaturized flow cell with pneumatically-actuated vertical nanoconfinement for single-molecule imaging and manipulation.

Biomicrofluidics 2018 Sep 28;12(5):054107. Epub 2018 Sep 28.

Department of Physics, McGill University, Montreal H3A 2T8, Canada.

Convex Lens-induced Confinement (CLiC) is a single-molecule imaging technique that uses a deformable glass flow cell to gently trap, manipulate, and visualize single molecules within micro- and nano-structures, to enable a wide range of applications. Here, we miniaturize the CLiC flow cell, from to and introduce pneumatic control of the confinement. Miniaturization of the flow cell improves fabrication throughput by almost two orders of magnitude and, advantageous for pharmaceutical and diagnostic applications where samples are precious, significantly lowers the internal volume from microliters to nanoliters. Read More

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http://aip.scitation.org/doi/10.1063/1.5052005
Publisher Site
http://dx.doi.org/10.1063/1.5052005DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6167230PMC
September 2018
3 Reads

Oral mucosa-on-a-chip to assess layer-specific responses to bacteria and dental materials.

Biomicrofluidics 2018 Sep 26;12(5):054106. Epub 2018 Sep 26.

Department of Biomedical Engineering, The Catholic University of America, 620 Michigan Avenue NE, Washington, District of Columbia 20064, USA.

The human oral mucosa hosts a diverse microbiome and is exposed to potentially toxic biomaterials from dental restoratives. Mucosal health is partly determined by cell and tissue responses to challenges such as dental materials and pathogenic bacteria. An model to rapidly determine potential layer-specific responses would lead to a better understanding of mucosal homeostasis and pathology. Read More

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http://dx.doi.org/10.1063/1.5048938DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6158033PMC
September 2018
35 Reads

Low temperature flow lithography.

Biomicrofluidics 2018 Sep 24;12(5):054105. Epub 2018 Sep 24.

Chemical and Biological Engineering, Korea University, Seoul 02841, Republic of Korea.

Flow lithography (FL) is a microfluidic technique distinguished for its ability to produce hydrogel microparticles of various geometrical and chemical designs. While FL is typically performed in room temperature, this paper reports a new technique called low temperature flow lithography that uses low synthesis temperature to increase the degree of polymerization of microparticles without compromising other aspects of flow lithography. We suggest that decreased oxygen diffusivity in low temperature is responsible for the increase in polymerization. Read More

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http://dx.doi.org/10.1063/1.5047016DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6153115PMC
September 2018

An integrated microfluidic platform for size-selective single-cell trapping of monocytes from blood.

Biomicrofluidics 2018 Sep 19;12(5):054104. Epub 2018 Sep 19.

Department of Biomedical Engineering, University of California at Irvine, Irvine, California 92967, USA.

Reliable separation and isolation of target single cells from bodily fluids with high purity is of great significance for an accurate and quantitative understanding of the cellular heterogeneity. Here, we describe a fully integrated single-blood-cell analysis platform capable of size-selective cell separation from a population containing a wide distribution of sizes such as diluted blood sample and highly efficient entrapment of single monocytes. The spiked single U937 cells (human monocyte cell line) are separated in sequence by two different-sized microfilters for removing large cell clumps, white blood cells, and red blood cells and then discriminated by dielectrophoretic force and isolated individually by downstream single-cell trapping arrays. Read More

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http://dx.doi.org/10.1063/1.5049149DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6145860PMC
September 2018
3 Reads