1,139 results match your criteria Biomeditsinskaia khimiia[Journal]


[Analysis of dihydroquercetin physical modification via in vitro and in silico methods].

Biomed Khim 2019 Feb;65(2):152-158

Lomonosov Moscow State University, Moscow, Russia.

Flavonoid-mediated materials are promising substances for the design of new functional materials because of their bioactivity, eco-friendliness, and cost-effectiveness. Dihydroquercetin (DHQ) is the major flavonoid in the wood of Larix dahurica Turcz. Previously some new modifications were created on the basis of DHQ, they were characterized by different morphological, physico-chemical and biopharmaceutical properties. Read More

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http://dx.doi.org/10.18097/PBMC20196502152DOI Listing
February 2019
1 Read

[Modeling and theoretical analysis of ring specific mimicry in view of isomerism within medicinal promising oligomers of "DIVEMA"].

Biomed Khim 2019 Feb;65(2):133-151

Topchiev Institute of Petrochemical Synthesis, RAS, Moscow, Russia; Research Center for Biomodulators, Health Research and Development Foundation, Moscow, Russia.

The furan or pyran related hetero cycles play basic role in structural units of nucleic acids (NA) and polysaccharides (PS), significantly predetermining their functional specifics. Some of such properties, in great relevancy for medicine, can be imitated through mimicry of polymers synthetic. Particularly, a formation of similar cycloisomeric chains is possible in process of free-radical cyclocopolymerization of divinyl ether (DVE) and maleic anhydride (MA). Read More

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http://dx.doi.org/10.18097/PBMC20196502133DOI Listing
February 2019
1 Read

[QSAR investigation of acute toxicity of organic compounds during oral administration to mice].

Biomed Khim 2019 Feb;65(2):123-132

Institute of Physiologically Active Compounds, Russian Academy of Sciences, Chernogolovka, Russia.

The effect of the structure of organic compounds on the acute toxicity upon oral injection in mice was studied using 2D simplex representation of the molecular structure and Random forest (RF) methods. Satisfactory quantitative structure-activity relationship (QSAR) models were constructed (R2 test = 0,61-0,62). The interpretation of the obtained QSAR models was carried out. Read More

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http://dx.doi.org/10.18097/PBMC20196502123DOI Listing
February 2019

[Xenobiotic toxicity prediction combined with xenobiotic metabolism prediction in the human body].

Biomed Khim 2019 Feb;65(2):114-122

Institute of Biomedical Chemistry, Moscow, Russia.

The majority of xenobiotics undergo a number of chemical reactions known as biotransformation in human body. The biological activity, toxicity, and other properties of the metabolites may significantly differ from those of the parent compound. Not only xenobiotic itself and its final metabolites produced in large quantities, but the intermediate and final metabolites that are formed in trace quantities, can cause undesirable effects. Read More

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http://dx.doi.org/10.18097/PBMC20196502114DOI Listing
February 2019

[QSAR-modeling of desoxyuridine triphosphatase inhibitors in a series of some derivatives of uracil].

Biomed Khim 2019 Feb;65(2):103-113

Bashkir State University, Ufa, Russia.

Due to the widespread prevalence, deoxyuridine triphosphatase (UTPase) is considered by modern biochemists and physicians as a promising target for the development of drugs with a wide range of activities. The therapeutic effect of these drugs will be due to suppression of DNA biosynthesis in various viruses, bacteria and protozoa. In order to rationalize the search for new dUTPase inhibitors, domestic and foreign researchers are actively using the QSAR methodology at the selection stage of hit compounds. Read More

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http://dx.doi.org/10.18097/PBMC20196502103DOI Listing
February 2019

[The prediction of the spectrum of biological activity and antimicrobial properties of diaminoazoles].

Biomed Khim 2019 Feb;65(2):99-102

Ivanovo State University of Chemistry and Technology, Ivanovo, Russia.

The work is devoted to predicting and studying biological properties of N-substituted analogs of 3,5-diamino-1,2,4-thiadiazole, which, in their turn, include in the composition of many drugs that exhibit a wide range of pharmacological actions. For searching of new alternative drugs with an antibacterial activity, but lacking resistance of microorganism strains to them, a computer screening of 2N-alkyl-substituted 5-amino-3-imino-1,2,4-thiadiazolines previously synthesized by us was carried out. The prediction of the spectrum of biological activity, as well as the determination of the probable toxicity of these compounds, was performed using the freely available computer programs PASS, Anti-Bac-Pred, and GUSAR. Read More

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http://dx.doi.org/10.18097/PBMC20196502099DOI Listing
February 2019

[Neural network modeling of multitarget RAGE inhibitory activity].

Biomed Khim 2019 Feb;65(2):91-98

Volgograd State Medical University, Volgograd, Russia.

Based on the methodology of artificial neural networks, models describing the dependence of the level of RAGE inhibitory activity on the affinity of compounds for target proteins of the RAGE-NF-kB signal pathway have been costructed. A validated database of the structures and activity levels of 183 known compounds, which were tested for RAGE inhibitory activity was formed. The analysis of the AGE-RAGE signaling pathways was carried out, 14 key RAGE-NF-kB signal pathway nodes were found, for which 34 relevant target proteins were identified. Read More

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http://dx.doi.org/10.18097/PBMC20196502091DOI Listing
February 2019
1 Read

[Podophyllotoxin analogue with bicyclo[3.2.1]octane moiety annelated with indole: synthesis, molecular modeling, and biological testing].

Biomed Khim 2019 Feb;65(2):86-90

Department of Chemistry, Lomonosov Moscow State University, Moscow, Russia; Institute of Physiologically active compounds, Russian Academy of Sciences, Chernogolovka, Russia.

C4-Ester derivatives of the anticancer agent podophyllotoxin with bridged moieties can either inhibit polymerization of alpha,beta-tubulin with the formation of microtubules (analogously to the parent molecule) or cause an unusual effect of "curling and shortening" of the microtubules (MT). In order to predict the effect of bridged podophyllotoxin derivatives on the MT network using computer molecular modeling it is desirable to enhance the structural diversity of their bridged substituents. In the present work we synthesized novel podophyllotoxin ester with bicyclo[3. Read More

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http://dx.doi.org/10.18097/PBMC20196502086DOI Listing
February 2019
1 Read

[Docking with combined use of a force field and a quantum-chemical method].

Biomed Khim 2019 Feb;65(2):80-85

"Dimonta Ltd"; Research Computer Center, Moscow State University, Moscow, Russia.

The paper presents the results concerning the application of docking programs FLM to combined use of the MMFF94 force field and the semiempirical quantum-chemical method PM7 in the docking procedure. At the first step of this procedure a fairly wide range of low-energy minima of the protein-ligand complex is found in the frame of the MMFF94 force field using the FLM program. The energies of all these minima are recalculated using the PM7 method and the COSMO solvent continuum model at the second step. Read More

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http://dx.doi.org/10.18097/PBMC20196502080DOI Listing
February 2019

[Discovering new antiretroviral compounds in "Big Data" chemical space of the SAVI library].

Biomed Khim 2019 Feb;65(2):73-79

Institute of Biomedical Chemistry, Moscow, Russia.

Despite significant advances in the application of highly active antiretroviral therapy, the development of new drugs for the treatment of HIV infection remains an important task because the existing drugs do not provide a complete cure, cause serious side effects and lead to the emergence of resistance. In 2015, a consortium of American and European scientists and specialists launched a project to create the SAVI (Synthetically Accessible Virtual Inventory) library. Its 2016 version of over 283 million structures of new easily synthesizable organic molecules, each annotated with a proposed synthetic route, were generated in silico for the purpose of searching for safer and more potent pharmacological substances. Read More

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http://dx.doi.org/10.18097/PBMC20196502073DOI Listing
February 2019

[Interaction of prostacyclin synthase with cytochromes P450].

Biomed Khim 2019 Jan;65(1):63-66

Institute of Biomedical Chemistry, Moscow, Russia.

Biosensor experiments on investigation of interaction between prostacyclin synthase (PGIS) and different proteins of the cytochrome P450 monooxygenase systems were perfomed. Interaction of PGIS with microsomal (CYP21A2, CYP2E1) and mitochondrial (CYP27A1, CYP11B1, CYP11B2, CYP11A1) cytochrome P450s was detected. Kinetic and equilibrium parameters of protein complexes formation were determined. Read More

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http://dx.doi.org/10.18097/PBMC20196501063DOI Listing
January 2019

[Expression of osteoprotegerin and soluble ligand of receptor of kappa-B transcription factor activator in the calcification of aortic valve].

Biomed Khim 2019 Jan;65(1):57-62

Almazov National Medical Research Centre, St. Petersburg, Russia.

The mechanism of valve calcification that is the main cause of aortic stenosis formation and progression is not yet clear. In recent years, the role of the OPG/RANKL/RANK system is considered as one of possible variants of pathogenesis of valve calcification. In presented work the differences in OPG and sRANKL levels involved in the calcification processes in tissues of patients with severe aortic stenosis have been examined. Read More

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http://dx.doi.org/10.18097/PBMC20196501057DOI Listing
January 2019
1 Read

[Protective action of a modified fragment of galanine in rats with doxorubicin-induced heart failure].

Biomed Khim 2019 Jan;65(1):51-56

National Medical Research Center for Cardiology, Moscow, Russia.

The use of the anticancer drug doxorubicin (Dox) is limited due to its cardiotoxic effect. Using the method of automatic solid-phase peptide synthesis, we obtained a synthetic agonist of galanin receptors GalR1-3 [RAla14, His15]-galanine (2-15) (G), exhibiting cardioprotective properties. It was purified by high performance liquid chromatography (HPLC). Read More

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http://dx.doi.org/10.18097/PBMC20196501051DOI Listing
January 2019
1 Read

[Preparation and characterization of a new mutant homolog of chemotaxis protein CheY from anaerobic hyperthermophilic microorganism Thermotoga naphthophila].

Biomed Khim 2019 Jan;65(1):41-50

Institute of Biomedical Chemistry, Moscow, Russia.

Using genetic engineering methods the expression vectors structures have been designed to produce recombinant proteins TnaCheY and Tna CheY-mut, the homologues of the chemotaxis protein CheY from the hyperthermophilic organism Thermotoga naphthophila in Escherichia coli BL21(DE3) cells. The cultivation conditions of transformed strains were optimized. The influence of episomal expression of the heterologous chemotaxis protein CheY on growth kinetics parameters of the culture of mesophilic bacteria E. Read More

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http://dx.doi.org/10.18097/PBMC20196501041DOI Listing
January 2019

[Inflammatory metabolites of arahidonic acid in tear fluid in UV-induced corneal damage].

Biomed Khim 2019 Jan;65(1):33-40

Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, Russia; Sechenov First Moscow State Medical University, Institute of Molecular Medicine, Moscow, Russia.

The ultraviolet (UV) B-induced damage of the eye surface of experimental animals (rabbits) includes loss of corneal epithelium, apoptosis of keratocytes and stromal edema. These changes are accompanied by clinically and histologically manifested corneal inflammation, neutrophil infiltration, and exudation of the anterior chamber of the eye. According to mass spectrometric analysis, UV-induced corneal damage is associated with pronounced changes in the lipid composition of tears, including a decrease in the amount of arachidonic acid and prostaglandin E2 and an increase in the concentrations of prostaglandin D2 and its derivative 15d-PGJ2. Read More

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http://dx.doi.org/10.18097/PBMC20196501033DOI Listing
January 2019
2 Reads

[Protective action of carnosine and organic lithium salts in case of ethanol-induced oxidative damage of proteins and lipids of blood plasma in healthy persons and alcoholic patients].

Biomed Khim 2019 Jan;65(1):28-32

Mental Health Research Institute, Tomsk National Research Medical Center, Russian Academy of Sciences,Tomsk, Russia.

Organic lithium salts containing anionic components (succinate, fumarate, pyruvate and antioxidant ascorbate) were tested for protection of blood plasma proteins and lipids against ethanol-induced oxidation in vitro. We used normothymic lithium carbonate and well-known antioxidant dipeptide carnosine (b-alanyl-L-histidine) as the reference drugs. The oxidized proteins and lipids were determined by the level of carbonylated proteins (CP) and TBA-reactive products (TBA-RP), respectively. Read More

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http://dx.doi.org/10.18097/PBMC20196501028DOI Listing
January 2019

[Neurotransmitter disturbances in some parts of the rat brain and their correction under chronic and intermittent alcohol intoxication].

Biomed Khim 2019 Jan;65(1):21-27

Grodno State Medical University, Grodno, Belarus.

The pool of key neuromediators and some neurotransmitter amino acids in cerebellum, hypothalamus and midbrain of rats exposed to chronic and different variants of interrupted alcohol intoxication was investigated. The most pronounced changes were recorded in midbrain. Chronic alcohol intoxication caused an increase in the concentrations of tyrosine, dopamine, 3,4-dihydroxyphenylacetic acid (DOPAC), noradrenaline, tryptophan, serotonin, GABA and aspartate in this part of the rat brain. Read More

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http://dx.doi.org/10.18097/PBMC20196501021DOI Listing
January 2019

[Search for genetic markers for precise diagnostics of keratoconus].

Biomed Khim 2019 Jan;65(1):9-20

Fyodorov Eye Microsurgery Complex Federal State Institution, Moscow, Russia.

Keratoconus is a chronic disorder of the cornea, characterized by its progressive thinning, stretching, and conical protrusion. Diagnostics of subclinical keratoconus, as well as its early stages (forme fruste), is a complex problem. The presence of these forms of keratoconus in a patient is one of the reasons for the development of keratectasia after laser refractive surgery. Read More

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http://dx.doi.org/10.18097/PBMC20196501009DOI Listing
January 2019

[Concentration of several osteotropic growth factors, markers of osteogenesis and biologically active molecules in the blood serum of patients with congenital pseudarthrosis of tibia during orthopaedic treatment with combined technologies].

Biomed Khim 2018 Nov;64(6):525-533

Russian Ilizarov Scientific Center for Restorative Traumatology and Orthopaedics, Kurgan, Russia.

Congenital pseudarthrosis of tibia is a genetic, systemic pathology with impaired bone remodeling and unknown pathogenetic mechanisms. Orthopaedic treatment of the disease can fail in some cases. The process of bone remodeling is known to occur under control of local and systemic growth factors, and we sought to explore several osteotropic growth factors, markers of osteogenesis and biologically active molecules in the blood serum of patients with congenital pseudarthrosis of tibia. Read More

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http://dx.doi.org/10.18097/PBMC20186406525DOI Listing
November 2018
6 Reads

[Targeted gene sequencing panels: applicability for neoantigen profiling of colon and rectal adenocarcinoma].

Biomed Khim 2018 Nov;64(6):517-524

Federal Research and Clinical Center of Physical-Chemical Medicine, Moscow, Russia.

Cancer immunotherapy represents a promising and rapidly developing approach for the treatment of oncological diseases. Among the methods of personalized adjuvant immunotherapy, neoantigenic peptide-based drugs have demonstrated substantial efficiency. These drugs are designed to target mutant proteins arising from somatic alterations in the genome of tumor cells and thus stimulate immune response against tumor tissues. Read More

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http://dx.doi.org/10.18097/PBMC20186406517DOI Listing
November 2018
12 Reads

[Central monoaminergic systems sensitivity to acute hypoxia with hypercapnia changes after the maintenance under the long-term social isolation].

Biomed Khim 2018 Nov;64(6):511-516

Institute of Experimental Medicine, St. Petersburg, Russia; Kirov Military Medical Academy, St. Petersburg, Russia.

The experiments were performed in male albino outbred mice kept in a group and under the conditions of long-term social isolation. The changes in the monoaminergic systems of the left and right hemispheres of the brain after acute hypoxia with hypercapnia have been studied. The levels of dopamine (DA), serotonin (5-HT) and their metabolites - dioxyphenylacetic (DOPAC), homovanillic (HVA), and 5-hydroxyindoleacetic (5-HIAA) acids - were determined by HPLC in the cerebral cortex, hippocampus and striatum of the right and left sides of the brain. Read More

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http://pbmc.ibmc.msk.ru/index.php/en/article/PBMC-2018-64-6-
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http://dx.doi.org/10.18097/PBMC20186406511DOI Listing
November 2018
2 Reads

[Increase of antituberculosis efficiency of rifampicin embedded into phospholipid nanoparticles with sodium oleate].

Biomed Khim 2018 Nov;64(6):505-510

Institute of Biomedical Chemistry, Moscow, Russia.

The formulation of the antituberculosis drug rifampicin embedded into 20-30 nm nanoparticles from soy phosphatidylcholine and sodium oleate, is characterized by greater bioavailability as compared with free drug substance. In this study higher antituberculosis activity of this formulation was shown. Rifampicin in nanoparticles demonstrated more effective inhibition of M. Read More

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http://dx.doi.org/10.18097/PBMC20186406505DOI Listing
November 2018

[Quantitative proteomics of human blood exosomes].

Biomed Khim 2018 Nov;64(6):496-504

Institute of Biomedical Chemistry, Moscow, Russia.

Exosomes are extracellular membrane vesicles secreted by cells into biological fluids. The outer membrane of exosomes protects their content from degradation and contains markers of the parent cell. Almost all cells of the body produce exosomes, however, tumor cells secrete them more intensively. Read More

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http://dx.doi.org/10.18097/PBMC20186406496DOI Listing
November 2018
10 Reads

[The role of sphingolipids in cardiovascular pathologies].

Biomed Khim 2018 Nov;64(6):487-495

Institute of Biochemical Physics, Russian Academy of Sciences, Moscow, Russia.

Cardiovascular diseases (CVD) remain the leading cause of death in industrialized countries. One of the most significant risk factors for atherosclerosis is hypercholesterolemia. Its diagnostics is based on routine lipid profile analysis, including the determination of total cholesterol, low and high density lipoprotein cholesterol, and triglycerides. Read More

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http://dx.doi.org/10.18097/PBMC20186406487DOI Listing
November 2018
1 Read

[The urokinase-type plasminogen activator system and its role in tumor progression].

Biomed Khim 2018 Nov;64(6):472-486

Institute of Biomedical Chemistry, Moscow, Russia.

In the multistage process of carcinogenesis, the key link in the growth and progression of the tumor is the invasion of malignant cells into normal tissue and their distribution and the degree of destruction of tissues. The most important role in the development of these processes is played by the system of urokinase-type plasminogen activator (uPA system), which consists of several components: serine proteinase - uPA, its receptor - uPAR and its two endogenous inhibitors - PAI-1 and PAI-2. The components of the uPA system are expressed by cancer cells to a greater extent than normal tissue cells. Read More

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http://pbmc.ibmc.msk.ru/index.php/en/article/PBMC-2018-64-6-
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http://dx.doi.org/10.18097/PBMC20186406472DOI Listing
November 2018
1 Read

[Antidiabetic role of high density lipoproteins].

Biomed Khim 2018 Nov;64(6):463-471

Institute of Biochemistry, Federal Research Center of Fundamental and Translation Medicine, Novosibirsk, Russia.

Disturbance in lipid metabolism can be both a cause and a consequence of the development of diabetes mellitus (DM). One of the most informative indicator of lipid metabolism is the ratio of atherogenic and antiatherogenic fractions of lipoproteins and their protein components. The review summarizes literature data and own results indicating the important role of high-density lipoprotein (HDL) and their main protein component, apolipoprotein A-I (apoA-I), in the pathogenesis of type 2 DM. Read More

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http://dx.doi.org/10.18097/PBMC20186406463DOI Listing
November 2018
10 Reads

[Pharmacokinetics of noopept and its active metabolite cycloprolyl glycine in rats].

Biomed Khim 2018 Sep;64(5):455-458

Zakusov Research Institute of Pharmacology, Moscow, Russia.

The study of the pharmacokinetics of new drugs and the identification of active metabolites is a necessary step for effective and safe use in the clinical practice. It is especially important for peptide drugs due to their enzymatic instability, low bioavailability and poor permeability through the blood-brain barrier (BBB). The role of endogenous neuropeptides containing cyclic amino acids, proline, pyroglutamic acid, and glycine, in the regulation of memory processes is known as terminal peptide fragments. Read More

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http://dx.doi.org/10.18097/PBMC20186405455DOI Listing
September 2018
2 Reads

[Chronic alcoholism influences the mRNA level of the orexin receptor type 1 (OX1R) in emotiogenic structures of the rat brain].

Biomed Khim 2018 Sep;64(5):451-454

Institute of Experimental Medicine, St. Petersburg, Russia; St. Petersburg State University, St. Petersburg, Russia; Kirov Military Medical Academy, St. Petersburg, Russia.

Orexin and its receptors were shown to be involved into mechanisms of pathological craving to alcohol. This paper demonstrates that the orexin receptor type 1 (OX1R) mRNA level significantly decreased in the prefrontal cortex of rats chronically (during 6 months) consuming ethanol compared with intact control. The same results were observed on day 1 and day 7 of alcohol withdrawal after chronic alcoholization. Read More

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http://dx.doi.org/10.18097/PBMC20186405451DOI Listing
September 2018
15 Reads

[Factors governing development of nonalcoholic fatty liver disease and insulin resistance in obesity].

Biomed Khim 2018 Sep;64(5):444-450

Immanuel Kant Baltic Federal University, Kaliningrad, Russia.

The factors promoting development of non-alcoholic fatty liver disease in patients with obesity and different state of carbohydrate metabolism have been studied. 43 patients were examined; these included 26 patients with abdominal obesity (BMI=52.9±7. Read More

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http://dx.doi.org/10.18097/PBMC20186405444DOI Listing
September 2018
2 Reads

[The role of modification of innate immunity in development of premature labore].

Biomed Khim 2018 Sep;64(5):439-443

Research Institute of Obstetrics and Pediatrics, Rostov State Medical University, Ministry of Health of Russia, Rostov-on-Don, Russia.

To clarify the role of the infectious factor in the development of premature birth (PB) in women in serum, the content of TLR-4, the p65 subunit of the nuclear factor NF-kB, the cytokine TNF-a and the activity of PLA2 (phospholipase A2) were determinated by the enzyme immunoassay was studied. The profile of bioregulators in women with premature births was characterized by a high content of TLR-4, TNF-a and an increase in PLA2 activity and a decrease in the activity of the p65 subunit of NF-kB. 89 women aged 20-39 years were examined at 34-40 weeks gestation. Read More

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http://dx.doi.org/10.18097/PBMC20186405439DOI Listing
September 2018
4 Reads

[Neutrophils as a source of factors that increase the length of the inflammatory phase of wound healing in patients with type 2 diabetes mellitus].

Biomed Khim 2018 Sep;64(5):433-438

Federal Research and Clinical Center of Physical-Chemical Medicine, Moscow, Russia.

Oxidative stress and neutrophil activation leading to an increase in myeloperoxidase (MPO), elastase and neutrophil extracellular trap (NET) levels in blood are considered as pathogenic mechanisms responsible for the development of extremity damage in people with type 2 diabetes mellitus (T2DM). The aim of this study was to analyze the relationship between factors, associated with neutrophil activation, and the length of the initial phase of wound healing (the inflammatory phase) in T2DM patients. Patients were divided retrospectively into three groups depending on the damage extent: group 1 (wound on toe) < group 2 (wound on foot) < group 3 (wound on lower leg). Read More

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http://dx.doi.org/10.18097/PBMC20186405433DOI Listing
September 2018
17 Reads

[The influence of humus acids of peloids and its components on free radical processes].

Biomed Khim 2018 Sep;64(5):429-432

Samara State Medical University, Samara, Russia.

The effect of individual components of humic substances of peloid on free radical oxidation processes has been investigated under conditions of oxidative stress induced in albino rats. Biological activity of peloids was determined using such parameters as the general antioxidant activity, activity superoxide dismutases, catalases and glutathione peroxidases on the third and tenth day of the experiment. Results indicate that the state of oxidative stress can be corrected on the third day of the experiment. Read More

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http://dx.doi.org/10.18097/PBMC20186405429DOI Listing
September 2018

[The effect of the neuroprotector isatin on complex formation of beta-amyloid peptide fragments with some intracellular proteins].

Biomed Khim 2018 Sep;64(5):423-428

Institute of Biomedical Chemistry, Moscow, Russia; Higher School of Economics, Moscow, Russia.

Amyloid-β peptide (1-42) (Aβ1-42) is a key player in the development and progression of Alzheimer's disease (AD) and related pathologies, determined by formation of protein aggregates in the central nervous system. Aβ1-42 binding to crucial intracellular targets (and their subsequent inactivation) obviously represents one of the earliest events preceding extracellular pathogenic oligomerization/aggregation of Aβ1-42. It is reasonable to expect that dissociation of the Aβ1-42 complexes with intracellular proteins by means of inhibitors followed by subsequent degradation of Aβ1-42 would not only protect critically important proteins but also prevent intracellular accumulation of Aβ1-42. Read More

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http://dx.doi.org/10.18097/PBMC20186405423DOI Listing
September 2018
1 Read

[Comparative analysis of gene expression in vascular cells of patients with advanced atherosclerosis].

Biomed Khim 2018 Sep;64(5):416-422

Research Institute of Medical Genetics, Tomsk National Research Medical Center, RAS, Tomsk, Russia; Siberian State Medical University, Tomsk, Russia.

In this study we performed a comparative gene expression analysis of carotid arteries in the area of atherosclerotic plaques and healthy internal mammary arteries of patients with advanced atherosclerosis by using microarray HumanHT-12 BeadChip ("Illumina"). The most down-regulated genes were APOD, FABP4, CIDEC and FOSB, and up-regulated gene was SPP1 (|FC|>64; pFDR<0.05). Read More

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http://dx.doi.org/10.18097/PBMC20186405416DOI Listing
September 2018
20 Reads

[Aryl-hydrocarbon receptor as a potential target for anticancer therapy].

Biomed Khim 2018 Sep;64(5):397-415

Koltzov Institute of Developmental Biology of Russian Academy of Sciences, Moscow, Russia.

Aryl-hydrocarbon receptor (Aryl Hydrocarbon Receptor, AHR) is a ligand-dependent transcription factor, whose functions are related to xenobiotic detoxification, response to inflammation, and maintenance of tissue homeostasis. Recent investigations suggest that AHR also plays an important role in the processes of carcinogenesis. Increased expression of AHR is observed in several types of tumors and tumor cell lines. Read More

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http://dx.doi.org/10.18097/PBMC20186405397DOI Listing
September 2018
17 Reads

[CLEC-2 induced signalling in blood platelets].

Biomed Khim 2018 Sep;64(5):387-396

Faculty of Physics, Lomonosov Moscow State University, Moscow, Russia; Center for Theoretical Problems of Physicochemical Pharmacology, Moscow, Russia.

Platelet activating receptor CLEC-2 has been identified on platelet surface a decade ago. The only confirmed endogenous CLEC-2 agonist is podoplanin. Podoplanin is a transmembrane protein expressed by lymphatic endothelial cells, reticular fibroblastic cells in lymph nodes, kidney podocytes and by cells of certain tumors. Read More

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http://dx.doi.org/10.18097/PBMC20186405387DOI Listing
September 2018

[Comparative characteristics of cytokine and IgE levels in patients with bronchial asthma of professional and non-professional genesis].

Biomed Khim 2018 Aug;64(4):376-379

East-Siberian Institute of Medico-Ecological Researches, Angarsk, Russia.

A comparative evaluation of serum concentrations of cytokines and IgE production in patients with occupational bronchial asthma (BA) caused by exposure to the toxic-dust factor of the aluminum industry and patients with asthma of unprofessional genesis was carried out. A more pronounced pro-inflammatory response has been found in patients with occupational BA characterized by hyperproduction of IL-1b, IL-8. While for persons with non-professional asthma, a decrease in IL-1b, IL-5, IL-10 is characteristic. Read More

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http://dx.doi.org/10.18097/PBMC20186404376DOI Listing
August 2018
1 Read

[Quantitative targeted screening of proteins associated with lung adenocarcinoma cancer by the method of selected reaction monitoring].

Biomed Khim 2018 Aug;64(4):368-375

Institute of Biomedical Chemistry, Moscow, Russia.

In the present study, we applied selected reaction monitoring (SRM) to a group of proteins that were previously reported to be associated with lung cancer (Novikova S.E. et al. Read More

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http://dx.doi.org/10.18097/PBMC20186404368DOI Listing
August 2018
1 Read

[The use of immunomodulators, antioxidants and hepatoprotectors for the correction of the liver, erythrocites and the immune system disorders in chronic ethanol intoxication].

Biomed Khim 2018 Aug;64(4):360-367

Kursk State Medical University, Kursk, Russia.

The effectiveness of three various combinations of an immunomodulator with an antioxidant and a membrane protector in the correction of metabolic and immune disorders has been studied in the experiment under 60-days ethanol intoxication. The development of such biochemical syndromes of the liver damage as cytolysis, intrahepatic, intracellular cholestasis, toxic liver damage by necrotic type, insufficiency of synthetic processes and inflammatory has been revealed. Oxidative stress development and the activation of lipid peroxidation on the systemic (blood plasma) and local level (erythrocytes) have been established. Read More

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http://pbmc.ibmc.msk.ru/index.php/en/article/PBMC-2018-64-4-
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http://dx.doi.org/10.18097/PBMC20186404360DOI Listing
August 2018
6 Reads

[The effect of deprenyl and isatin administration to mice on the proteomic profile of liver isatin-binding proteins].

Biomed Khim 2018 Aug;64(4):354-359

Institute of Biomedical Chemistry, Moscow, Russia.

Isatin (indol-2,3-dione) is an endogenous indole found in the brain, peripheral tissues and biological body fluids of humans and animals. Its wide spectrum of biological activity is realized via interaction with numerous isatin-binding proteins; these include proteins playing an important role in the development of neurodegenerative pathology. In the context of the neuroprotective effect, the effect of isatin is comparable to the effects of deprenyl, a pharmacological agent used for treatment of Parkinson's disease. Read More

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http://dx.doi.org/10.18097/PBMC20186404354DOI Listing
August 2018
1 Read

[Prooxidant-antioxidant balance depending on endothelial nitric oxide synthase G894T polymorphism].

Biomed Khim 2018 Aug;64(4):349-353

Grodno State Medical University, Grodno, Belarus.

The aim of the study was to assess the prooxidant-antioxidant balance depending on endothelial nitric oxide synthase G894T polymorphism. The frequency distribution of alleles and genotypes of G894T polymorphism, nitrite concentration, hydrogen sulphide, lipid peroxidation products (diene conjugates, malonic dialdehyde), antioxidants (reduced glutathione, catalase, ceruloplasmin, retinol) were determined in venous blood of healthy males. The incidence of the GG genotype was 49. Read More

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http://dx.doi.org/10.18097/PBMC20186404349DOI Listing
August 2018
1 Read

[Study of the neuroprotective effects of carnosine in an experimental model of focal cerebral ischemia/reperfusion].

Biomed Khim 2018 Aug;64(4):344-348

Federal Research Centre of Nutrition, Biotechnology and Food Safety, Moscow, Russia.

Oxidative stress is one of the key factors in brain tissue damage in ischemia, which indicates the appropriateness of using antioxidants under these conditions. One of the promising antioxidants for the therapy of ischemic stroke is the natural dipeptide carnosine. The neuroprotective effect of dietary carnosine administration was investigated in an experimental model of focal cerebral ischemia/reperfusion in Wistar rats. Read More

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http://dx.doi.org/10.18097/PBMC20186404344DOI Listing
August 2018
1 Read

[Influence of the dense extract from herb of Primula veris L. On the oxidative stress development and the functional state of the cardiomyocytes mitochondria of rats with experimental chronic heart failure].

Biomed Khim 2018 Aug;64(4):334-343

Bashkir State Medical University, Ufa, Russia.

Experimental chronic heart failure (CHF), caused by administration of L-isoproterenol (2.5 mg/kg twice a day intraperitoneally for 21 days), promotes uncoupling of respiration and oxidative phosphorylation. The rate of mitochondrial oxygen consumption in the metabolic state V3 by Chance in animals with CHF decreased by 53. Read More

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http://dx.doi.org/10.18097/PBMC20186404334DOI Listing
August 2018
10 Reads

[Early changes in hyppocampal neurogenesis induced by soluble Ab1-42 oligomers].

Biomed Khim 2018 Aug;64(4):326-333

Department of Biochemistry, Medical, Pharmaceutical, and Toxicological Chemistry, Krasnoyarsk State Medical University named after Prof. V.F. Voino-Yasenetsky, Krasnoyarsk, Russia; Research Institute of Molecular Medicine and Pathobiochemistry, Krasnoyarsk State Medical University named after Prof. V.F. Voino-Yasenetsky, Krasnoyarsk, Russia.

Alzheimer's disease is characterized by the loss of neurons, the accumulation of intracellular neurofibrillary tangles and extracellular amyloid plaques in the brain. However, there are contradicting data on differences in neurogenesis at the onset of the disease or before the formation of amyloid plaques. As awareness of the importance of the pre-symptom phase in neurodegenerative diseases grows in the context of early diagnosis and pathogenesis, we analyzed the critical periods of adult hippocampal neurogenesis at an early stage under the action of soluble Ab1-42 beta-amyloid. Read More

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http://dx.doi.org/10.18097/PBMC20186404326DOI Listing
August 2018
16 Reads

[Genetic and biochemical features of the monogenic hereditary urolithiasis].

Biomed Khim 2018 Aug;64(4):315-325

Institute of Molecular Medicine of the Sechenov First Moscow State Medical University (Sechenov University), Moscow, Russia; Research Centre for Medical Genetics, Moscow, Russia.

Urolithiasis is a common urological problem. In most cases, this multifactorial pathology develops due to the combination of inherited low-penetrance gene variants and environment factors such as urinary tract infections and unbalanced diet. However, some cases are monogenic. Read More

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http://dx.doi.org/10.18097/PBMC20186404315DOI Listing
August 2018
22 Reads

[Searching for essential genes in cancer genomes].

Biomed Khim 2018 Aug;64(4):303-314

Institute of Biomedical Chemistry, Moscow, Russia; Pirogov Russian National Research Medical University, Moscow, Russia.

The concept of essential genes, whose loss of functionality leads to cell death, is one of the fundamental concepts of genetics and is important for fundamental and applied research. This field is particularly promising in relation to oncology, since the search for genetic vulnerabilities of cancer cells allows us to identify new potential targets for antitumor therapy. The modern biotechnology capacities allow carrying out large-scale projects for sequencing somatic mutations in tumors, as well as directly interfering the genetic apparatus of cancer cells. Read More

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http://dx.doi.org/10.18097/PBMC20186404303DOI Listing

[Features of redox processes in the amniotic fluid at placental insufficiency].

Biomed Khim 2018 Jun;64(3):290-297

Federal Research Centre Southern Scientific Center of Russian Academy of Sciences, Rostov-on-Don, Russia.

Activity of prooxidant enzymes (NADPH-oxidase and xanthine oxidase), antioxidant enzymes (superoxide dismutase (SOD) and catalase), enzymes of the glutathione-dependent systems, as well as antioxidant vitamins (retinol and a-tocopherol), lipid peroxidation products (LPP) (conjugated dienes and Schiff bases), and peroxide chemiluminescence were studied in the amniotic fluid at different periods of physiological pregnancy and placental insufficiency (PI). It was found that at PI the activity of NADPH-oxidase, xanthine oxidase increased and the activity of SOD, catalase, glutathione peroxidase, glutathione reductase, glutathione transferase and the content of fat-soluble vitamins decreased. The direct and inverse correlation between the studied pro- and antioxidant parameters and the content of LPP products, was found ro be different in the II and III trimesters of gestation. Read More

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http://dx.doi.org/10.18097/PBMC20186403290DOI Listing
June 2018
7 Reads

[Primary screening of substances-photosensibilizers of the bacteriochlorin range for photodynamic therapy of malignant neoplasms].

Biomed Khim 2018 Jun;64(3):283-289

Hertsen Moscow Institute of Oncology, Moscow, Russia.

This paper presents a primary screening of bacteriochlorin-type compounds with aminoamide, propyl and carbohydrate substituents aimed for development a new generation photosensitizers (PS) for photodynamic therapy of malignant tumors. Absorption and fluorescence spectral characteristics of the compounds, their storage stability in solutions under dark conditions and light exposure, photo-induced and dark cytotoxicity against human HEp2 tumor cells have been studied. It has been shown that the dyes with aminoamide substituents have an absorbtion maximum at 754±2 nm in the long wavelength region and they are not stable during storage (the specific fluorescence intensity decreased by 33-56% during 24 hours). Read More

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http://dx.doi.org/10.18097/PBMC20186403283DOI Listing
June 2018
13 Reads

[A rational approach to obtaining high-specific polyclonal antibodies against recombinant alpha-synuclein].

Biomed Khim 2018 Jun;64(3):276-282

Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, Russia; Faculty of Bioengineering and Bioinformatics, Lomonosov Moscow State University, Moscow, Russia.

The approach for the quick and efficient production ofpolyclonal antibodies tothe target antigen alpha-synuclein has been proposed. Two methods have been employed to purify specific rabbit polyclonal antibodies against recombinant human alpha-synuclein, produced by subcutaneous immunization with complete Freund's adjuvant. It was shown that purification on CNBr-activated Sepharose with immobilized alpha-synuclein resulted in antibody preparation with rabbit serum histidine-rich glycoprotein as a contaminant. Read More

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http://dx.doi.org/10.18097/PBMC20186403276DOI Listing
June 2018
15 Reads

[Lipoilcarnosine: synthesis, study of physico-chemical and antioxidant properties, biological activity].

Biomed Khim 2018 Jun;64(3):268-275

Moscow Technological University, Moscow, Russia.

Synthesis of lipoilcarnosine (LipC) - a conjugated molecule based on two natural antioxidants, carnosine and a-lipoic acid, is described. Its physico-chemical, antioxidant properties and biological activity are characterized. According to reversed-phase HPLC with a UV detector, purity of the final product was 89. Read More

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http://dx.doi.org/10.18097/PBMC20186403268DOI Listing
June 2018
4 Reads