503 results match your criteria Biomedicines[Journal]


Transcriptome-Wide Analysis of Deficient Retinal Pigment Epithelial (RPE) Cells Reveals Molecular Signatures of RPE Homeostasis.

Biomedicines 2020 Jun 1;8(6). Epub 2020 Jun 1.

Department of Ophthalmology, University of Missouri, Columbia, MO 65212, USA.

Age-related macular degeneration (AMD) is the most common cause of irreversible blindness in the elderly population. In our previous studies, we found that deficiency of causes AMD-like pathological phenotypes in mice, characterized by abnormalities and dysfunction of the retinal pigment epithelium (RPE) cells. The abnormalities included abnormal cellular shape and impaired barrier function. Read More

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http://dx.doi.org/10.3390/biomedicines8060147DOI Listing

Probiotics-Containing Yogurt Ingestion and Eradication Can Restore Fecal Dysbiosis in -Infected Children.

Biomedicines 2020 Jun 1;8(6). Epub 2020 Jun 1.

Departments of Internal Medicine, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan 70428, Taiwan.

This study investigated the compositional differences in fecal microbiota between children with and without infection and tested whether probiotics-containing yogurt and bacterial eradication improve -related dysbiosis. Ten -infected children and 10 controls ingested probiotics-containing yogurt for 4 weeks. Ten-day triple therapy plus yogurt was given to the infected children on the 4th week. Read More

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http://dx.doi.org/10.3390/biomedicines8060146DOI Listing

Tyramine and Amyloid Beta 42: A Toxic Synergy.

Biomedicines 2020 May 30;8(6). Epub 2020 May 30.

School of Science, RMIT University, Bundoora, VIC 3083, Australia.

Implicated in various diseases including Parkinson's disease, Huntington's disease, migraines, schizophrenia and increased blood pressure, tyramine plays a crucial role as a neurotransmitter in the synaptic cleft by reducing serotonergic and dopaminergic signaling through a trace amine-associated receptor (TAAR1). There appear to be no studies investigating a connection of tyramine to Alzheimer's disease. This study aimed to examine whether tyramine could be involved in AD pathology by using expressing Aβ42. Read More

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http://dx.doi.org/10.3390/biomedicines8060145DOI Listing

Higher Epoxyeicosatrienoic Acids in Cardiomyocytes-Specific CYP2J2 Transgenic Mice Are Associated with Improved Myocardial Remodeling.

Biomedicines 2020 May 30;8(6). Epub 2020 May 30.

Department of Medicinal Chemistry, University of Washington, Box 357610, Seattle, WA 98195, USA.

Elevated epoxyeicosatrienoic acids (EETs) are known to be cardioprotective during ischemia-reperfusion injury in cardiomyocyte-specific overexpressing cytochrome P450 2J2 (CYP2J2) transgenic (Tr) mice. Using the same Tr mice, we measured changes in cardiac and erythrocyte membranes EETs following myocardial infarction (MI) to determine if they can serve as reporters for cardiac events. Cardiac function was also assessed in Tr vs. Read More

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http://dx.doi.org/10.3390/biomedicines8060144DOI Listing

Caterpillar Venom: A Health Hazard of the 21st Century.

Biomedicines 2020 May 30;8(6). Epub 2020 May 30.

Toxicology and Pharmacology, KU Leuven, Campus Gasthuisberg, O & N2, Herestraat 49, P.O. Box 922, 3000 Leuven, Belgium.

Caterpillar envenomation is a global health threat in the 21st century. Every direct or indirect contact with the urticating hairs of a caterpillar results in clinical manifestations ranging from local dermatitis symptoms to potentially life-threatening systemic effects. This is mainly due to the action of bioactive components in the venom that interfere with targets in the human body. Read More

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http://dx.doi.org/10.3390/biomedicines8060143DOI Listing

RNA Sequencing-Based Identification of Ganglioside GD2-Positive Cancer Phenotype.

Biomedicines 2020 May 30;8(6). Epub 2020 May 30.

Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 16/10, Miklukho- Maklaya St., 117997 Moscow, Russia.

The tumor-associated ganglioside GD2 represents an attractive target for cancer immunotherapy. GD2-positive tumors are more responsive to such targeted therapy, and new methods are needed for the screening of GD2 molecular tumor phenotypes. In this work, we built a gene expression-based binary classifier predicting the GD2-positive tumor phenotypes. Read More

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http://dx.doi.org/10.3390/biomedicines8060142DOI Listing

Anti-Anaphylactic Activity of Isoquercitrin (Quercetin-3-O-β-d-Glucose) in the Cardiovascular System of Animals.

Authors:
Jinbong Park

Biomedicines 2020 May 29;8(6). Epub 2020 May 29.

Department of Pharmacology, College of Korean Medicine, Kyung Hee University, Seoul 02447, Korea.

Effects of isoquercitrin (IQ) on anaphylactic responses were examined in cardiovascular systems of experimental animals. In pithed rats, IQ at 30 and 100 mg/kg (intravenous) significantly blunted both the initial hypertensive and the ensuing hypotensive responses during anaphylaxis. Death rate and tachycardia were also significantly inhibited after the same IQ doses in these rats. Read More

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http://dx.doi.org/10.3390/biomedicines8060139DOI Listing

Identification of Novel Native Autoantigens in Rheumatoid Arthritis.

Biomedicines 2020 May 29;8(6). Epub 2020 May 29.

Department of Health Science and Technology, Aalborg University, 9220 Aalborg, Denmark.

The majority of patients diagnosed with rheumatoid arthritis (RA) have developed autoantibodies against neoepitopes in proteins that have undergone post-translational modification, e.g., citrullination or carbamylation. Read More

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http://dx.doi.org/10.3390/biomedicines8060141DOI Listing

Synthesis and In Vitro Photodynamic Activity of Cationic Boron Dipyrromethene-Based Photosensitizers against Methicillin-Resistant .

Biomedicines 2020 May 29;8(6). Epub 2020 May 29.

Department of Chemistry, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong.

A series of cationic boron dipyrromethene (BODIPY) derivatives were synthesized and characterized with various spectroscopic methods. Having the ability to generate singlet oxygen upon irradiation, these compounds could potentially serve as photosensitizers for antimicrobial photodynamic therapy. Of the five BODIPYs being examined, the dicationic aza-BODIPY analogue (compound ) demonstrated the highest potency against a broad spectrum of clinically relevant methicillin-resistant (MRSA), including four ATCC-type strains (ATCC 43300, ATCC BAA-42, ATCC BAA-43, and ATCC BAA-44), two strains carrying specific antibiotic resistance mechanisms [-AAC(6')-APH(2") and RN4220/pUL5054], and ten non-duplicate clinical strains from hospital- and community-associated MRSAs of the important clonal types ST239, ST30, and ST59, which have previously been documented to be prevalent in Hong Kong and its neighboring countries. Read More

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http://dx.doi.org/10.3390/biomedicines8060140DOI Listing

Javamide-II Inhibits IL-6 without Significant Impact on TNF-alpha and IL-1beta in Macrophage-Like Cells.

Biomedicines 2020 May 29;8(6). Epub 2020 May 29.

Diet, Genomics, and Immunology Laboratory, Bldg. 307C, Rm. 131, BHNRC, ARS, USDA, Beltsville, MD 20705, USA.

The main aim of this study is to find a therapeutic compound to inhibit IL-6, not TNF-alpha and IL-1beta, in macrophage-like cells, because the high-levels of IL-6 production by macrophages are reported to cause unfavorable outcomes under several disease conditions (e.g., autoimmune diseases, and acute viral infections, including COVID-19). Read More

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http://dx.doi.org/10.3390/biomedicines8060138DOI Listing

Endocrine-Disrupting Chemicals and Insulin Resistance in Children.

Biomedicines 2020 May 28;8(6). Epub 2020 May 28.

Department of Pediatrics, University of Chieti, I-66100 Chieti, Italy.

The purpose of this article is to review the evidence linking background exposure to endocrine-disrupting chemicals (EDCs) with insulin resistance in children. Although evidence in children is scarce since very few prospective studies exist even in adults, evidence that EDCs might be involved in the development of insulin resistance and related diseases such as obesity and diabetes is accumulating. We reviewed the literature on both cross-sectional and prospective studies in humans and experimental studies. Read More

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http://dx.doi.org/10.3390/biomedicines8060137DOI Listing

Ataxia as the Major Manifestation of Fragile X-Associated Tremor/Ataxia Syndrome (FXTAS): Case Series.

Biomedicines 2020 May 25;8(5). Epub 2020 May 25.

Department of Pediatrics, University of California Davis School of Medicine, Sacramento, CA 95817, USA.

Fragile X-associated tremor and ataxia syndrome (FXTAS) is a neurodegenerative disease developed by carriers of a premutation in the fragile X mental retardation 1 ( gene. The core clinical symptoms usually manifest in the early 60s, typically beginning with intention tremor followed by cerebellar ataxia. Ataxia can be the only symptom in approximately 20% of the patients. Read More

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http://dx.doi.org/10.3390/biomedicines8050136DOI Listing

Comprehensive Exonic Sequencing of Known Ataxia Genes in Episodic Ataxia.

Biomedicines 2020 May 25;8(5). Epub 2020 May 25.

Genomics Research Centre, Institute of Health and Biomedical Innovation (IHBI), School of Biomedical Sciences, Q Block, Queensland University of Technology (QUT), 60 Musk Ave, Kelvin Grove Campus, Brisbane, Queensland 4059, Australia.

Episodic Ataxias (EAs) are a small group (EA1-EA8) of complex neurological conditions that manifest as incidents of poor balance and coordination. Diagnostic testing cannot always find causative variants for the phenotype, however, and this along with the recently proposed EA type 9 (EA9), suggest that more EA genes are yet to be discovered. We previously identified disease-causing mutations in the gene in 48% ( = 15) of 31 patients with a suspected clinical diagnosis of EA2, and referred to our laboratory for gene testing, leaving 52% of these cases ( = 16) with no molecular diagnosis. Read More

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http://dx.doi.org/10.3390/biomedicines8050134DOI Listing

(Synn. ) Polysaccharides in Cancer Therapy: Targets and Efficacy.

Biomedicines 2020 May 25;8(5). Epub 2020 May 25.

Pharmacognosy Research Laboratories & Herbal Analysis Services UK, University of Greenwich, Chatham-Maritime, Kent ME4 4TB, UK.

(L.) Quél. is a higher fungi or mushroom which is now known by its accepted scientific name as (L. Read More

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http://dx.doi.org/10.3390/biomedicines8050135DOI Listing

Evaluation of Aptamers as Affinity Reagents for an Enhancement of SRM-Based Detection of Low-Abundance Proteins in Blood Plasma.

Biomedicines 2020 May 24;8(5). Epub 2020 May 24.

Institute of Biomedical Chemistry, Moscow 119121, Russia.

Selected reaction monitoring (SRM) is a mass spectrometric technique characterized by the exceptionally high selectivity and sensitivity of protein detection. However, even with this technique, the quantitative detection of low- and ultralow-abundance proteins in blood plasma, which is of great importance for the search and verification of novel protein disease markers, is a challenging task due to the immense dynamic range of protein abundance levels. One approach used to overcome this problem is the immunoaffinity enrichment of target proteins for SRM analysis, employing monoclonal antibodies. Read More

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http://dx.doi.org/10.3390/biomedicines8050133DOI Listing

Brassicasterol with Dual Anti-Infective Properties against HSV-1 and , and Cardiovascular Protective Effect: Nonclinical In Vitro and In Silico Assessments.

Biomedicines 2020 May 24;8(5). Epub 2020 May 24.

Department of Applied Ecology, Faculty of Environmental Sciences, Czech University of Life Sciences Prague, Kamýcká 129, 6-Suchdol, 16521 Prague, Czech Republic.

While few studies have revealed the biological properties of brassicasterol, a phytosterol, against some biological and molecular targets, it is believed that there are still many activities yet to be studied. In this work, brassicasterol exerts a therapeutic utility in an in vitro setting against herpes simplex virus type 1 (HSV-1) and (Mtb) as well as a considerable inhibitory property against human angiotensin-converting enzyme (ACE) that plays a dynamic role in regulating blood pressure. The antireplicative effect of brassicasterol against HSV-1 is remarkably detected (50% inhibitory concentration (IC): 1. Read More

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http://dx.doi.org/10.3390/biomedicines8050132DOI Listing

When Less Is More: Specific Capture and Analysis of Tumor Exosomes in Plasma Increases the Sensitivity of Liquid Biopsy for Comprehensive Detection of Multiple Androgen Receptor Phenotypes in Advanced Prostate Cancer Patients.

Biomedicines 2020 May 22;8(5). Epub 2020 May 22.

Oncology Unit, Department of Medical and Surgical Specialties, Radiological Sciences and Public Health, University of Brescia, ASST Spedali Civili of Brescia, 25123 Brescia, Italy.

We evaluated the advantages and the reliability of novel protocols for the enrichment of tumor extracellular vesicles (EVs), enabling a blood-based test for the noninvasive parallel profiling of multiple androgen receptor ( gene alterations. Three clinically relevant variants related to response/resistance to standard-of-care treatments (-V7 transcript, T878A point mutation and gene amplification) were evaluated by digital PCR in 15 samples from patients affected by Castration-Resistant Prostate Cancer (CRPC). Plasma was processed to obtain circulating RNA and DNA using protocols based on tumor EVs enrichment through immuno-affinity and peptide-affinity compared to generic extraction kits. Read More

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http://dx.doi.org/10.3390/biomedicines8050131DOI Listing

Spinal Cord Involvement in MS and Other Demyelinating Diseases.

Biomedicines 2020 May 22;8(5). Epub 2020 May 22.

Neurology Department, Fleni, C1428AQK Buenos Aires, Argentina.

Diagnostic accuracy is poor in demyelinating myelopathies, and therefore a challenge for neurologists in daily practice, mainly because of the multiple underlying pathophysiologic mechanisms involved in each subtype. A systematic diagnostic approach combining data from the clinical setting and presentation with magnetic resonance imaging (MRI) lesion patterns, cerebrospinal fluid (CSF) findings, and autoantibody markers can help to better distinguish between subtypes. In this review, we describe spinal cord involvement, and summarize clinical findings, MRI and diagnostic characteristics, as well as treatment options and prognostic implications in different demyelinating disorders including: multiple sclerosis (MS), neuromyelitis optica spectrum disorder, acute disseminated encephalomyelitis, anti-myelin oligodendrocyte glycoprotein antibody-associated disease, and glial fibrillary acidic protein IgG-associated disease. Read More

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http://dx.doi.org/10.3390/biomedicines8050130DOI Listing

Tripartite Combination of Candidate Pandemic Mitigation Agents: Vitamin D, Quercetin, and Estradiol Manifest Properties of Medicinal Agents for Targeted Mitigation of the COVID-19 Pandemic Defined by Genomics-Guided Tracing of SARS-CoV-2 Targets in Human Cells.

Biomedicines 2020 May 21;8(5). Epub 2020 May 21.

Institute of Engineering in Medicine, University of California, San Diego, 9500 Gilman Dr. MC 0435, La Jolla, CA 92093-0435, USA.

Genes required for SARS-CoV-2 entry into human cells, ACE2 and FURIN, were employed as baits to build genomic-guided molecular maps of upstream regulatory elements, their expression and functions in the human body, and pathophysiologically relevant cell types. Repressors and activators of the ACE2 and FURIN genes were identified based on the analyses of gene silencing and overexpression experiments as well as relevant transgenic mouse models. Panels of repressors () and activators () were then employed to identify existing drugs manifesting in their effects on gene expression signatures of potential coronavirus infection mitigation agents. Read More

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http://dx.doi.org/10.3390/biomedicines8050129DOI Listing

Characterisation of a Novel A-Superfamily Conotoxin.

Biomedicines 2020 May 20;8(5). Epub 2020 May 20.

Centre for Molecular Therapeutics, Australian Institute of Tropical Health and Medicine, James Cook University, Smithfield, QLD 4878, Australia.

Conopeptides belonging to the A-superfamily from the venomous molluscs, , are typically α-conotoxins. The α-conotoxins are of interest as therapeutic leads and pharmacological tools due to their selectivity and potency at nicotinic acetylcholine receptor (nAChR) subtypes. Structurally, the α-conotoxins have a consensus fold containing two conserved disulfide bonds that define the two-loop framework and brace a helical region. Read More

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http://dx.doi.org/10.3390/biomedicines8050128DOI Listing

Assessment of the Antitumor Potential of Umbelliprenin, a Naturally Occurring Sesquiterpene Coumarin.

Biomedicines 2020 May 18;8(5). Epub 2020 May 18.

Department of Science in Korean Medicine, College of Korean Medicine, Kyung Hee University, 24 Kyungheedae-ro, Dongdaemun-gu, Seoul 02447, Korea.

Cancer is one of the greatest causes of mortality worldwide. The prevalence rates of different types of cancer is increasing around the world as well. Limitations in chemotherapy and radiotherapy, owing to multiple side effects including cytotoxic effects of antitumor compounds on normal cells as well as the development of resistance to these treatment options in patients, create a serious threat to successful treatment of cancer. Read More

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http://dx.doi.org/10.3390/biomedicines8050126DOI Listing

Organic Cation Transporters (OCTs) in EpiAirway™, A Cellular Model of Normal Human Bronchial Epithelium.

Biomedicines 2020 May 19;8(5). Epub 2020 May 19.

Laboratory of General Pathology, Department of Medicine and Surgery, University of Parma, 43125 Parma, Italy.

Organic cation transporters (OCTs) and novel organic cation transporters (OCTNs) are responsible for drug delivery in the intestine and kidney; in the lung, OCTs mediate inhaled drugs' transport, although their physiological role in airways remains poorly understood. The studies addressing OCTs/OCTNs in human airways were mostly performed in immortal or transformed cell lines; here, we studied OCTs in EpiAirway™, a recently developed in vitro model of normal bronchial epithelium. Calu-3 monolayers were used for comparison. Read More

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http://dx.doi.org/10.3390/biomedicines8050127DOI Listing

Inflammatory and Oxidative Stress Markers-Mirror Tools in Rheumatoid Arthritis.

Biomedicines 2020 May 15;8(5). Epub 2020 May 15.

Department of Biochemistry, University of Medicine and Pharmacy of Craiova, 200349 Craiova, Romania.

Rheumatoid arthritis (RA) is a chronic progressive autoimmune disease, associated with significant morbidity, mainly due to progressive damage and consequent disability. Oxidative stress is an important part of RA pathophysiology, as in autoimmune disease the interaction between immune response and endogenous/exogenous antigens subsequently induce the production of reactive oxygen species. The oxidative stress process seems to be positively strongly correlated with inflammation and accelerated joint destruction. Read More

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http://dx.doi.org/10.3390/biomedicines8050125DOI Listing

Sex Differences in Biological Processes and Nitrergic Signaling in Mouse Brain.

Biomedicines 2020 May 15;8(5). Epub 2020 May 15.

Institute for Drug Research, School of Pharmacy, Faculty of Medicine, The Hebrew University of Jerusalem, 9112102 Jerusalem, Israel.

Nitric oxide (NO) represents an important signaling molecule which modulates the functions of different organs, including the brain. S-nitrosylation (SNO), a post-translational modification that involves the binding of the NO group to a cysteine residue, is a key mechanism of nitrergic signaling. Most of the experimental studies are carried out on male animals. Read More

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http://dx.doi.org/10.3390/biomedicines8050124DOI Listing

Low-Grade Inflammation Is Not Present in Former Obese Males but Adipose Tissue Macrophage Infiltration Persists.

Biomedicines 2020 May 14;8(5). Epub 2020 May 14.

Xlab, Center for Healthy Aging, Department of Biomedical Sciences, University of Copenhagen, 2200N Copenhagen, Denmark.

Macrophage infiltration in two subcutaneous adipose tissue depots and systemic low-grade inflammation were studied in post-obese (PO), obese (O), and control (C) subjects. Young males were recruited into PO: ( = 10, weight-loss avg. 26%, BMI: 26. Read More

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http://dx.doi.org/10.3390/biomedicines8050123DOI Listing

Synthesis and Modeling Studies of Furoxan Coupled Spiro-Isoquinolino Piperidine Derivatives as NO Releasing PDE 5 Inhibitors.

Biomedicines 2020 May 14;8(5). Epub 2020 May 14.

School of Chemical Sciences, SRTM University, Nanded 431606, Maharashtra, India.

Nitric oxide (NO) is considered to be one of the most important intracellular messengers that play an active role as neurotransmitter in regulation of various cardiovascular physiological and pathological processes. Nitric oxide (NO) is a major factor in penile erectile function. NO exerts a relaxing action on corpus cavernosum and penile arteries by activating smooth muscle soluble guanylate cyclase and increasing the intracellular concentration of cyclic guanosine monophosphate (cGMP). Read More

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http://dx.doi.org/10.3390/biomedicines8050121DOI Listing

Flavolignans from Silymarin as Nrf2 Bioactivators and Their Therapeutic Applications.

Biomedicines 2020 May 14;8(5). Epub 2020 May 14.

Laboratorio de Medicina de Conservación, Escuela Superior de Medicina, Instituto Politécnico Nacional, México Escuela Superior de Medicina, Plan de San Luis y Díaz Mirón, Col. Casco de Santo Tomás, Alcaldía Miguel Hidalgo, Mexico City 11340, Mexico.

Silymarin (SM) is a mixture of flavolignans extracted from the seeds of species derived from , commonly known as milk thistle or St. Mary'sthistle. These species have been widely used in the treatment of liver disorders in traditional medicine since ancient times. Read More

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http://dx.doi.org/10.3390/biomedicines8050122DOI Listing

Non-Invasive Delivery of Therapeutics into the Brain: The Potential of Aptamers for Targeted Delivery.

Biomedicines 2020 May 14;8(5). Epub 2020 May 14.

School of Medicine, Deakin University, Geelong, VIC 3216, Australia.

The blood-brain barrier (BBB) is a highly specialised network of blood vessels that effectively separates the brain environment from the circulatory system. While there are benefits, in terms of keeping pathogens from entering the brain, the BBB also complicates treatments of brain pathologies by preventing efficient delivery of macromolecular drugs to diseased brain tissue. Although current non-invasive strategies of therapeutics delivery into the brain, such as focused ultrasound and nanoparticle-mediated delivery have shown various levels of successes, they still come with risks and limitations. Read More

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http://dx.doi.org/10.3390/biomedicines8050120DOI Listing

Discovery of Hordenine as a Potential Inhibitor of Pyruvate Dehydrogenase Kinase 3: Implication in Lung Cancer Therapy.

Biomedicines 2020 May 14;8(5). Epub 2020 May 14.

Centre for Interdisciplinary Research in Basic Sciences, Jamia Millia Islamia, Jamia Nagar, New Delhi 110025, India.

Design and development of potential pyruvate dehydrogenase kinase 3 (PDK3) inhibitors have gained attention because of their possible therapeutic uses in lung cancer therapy. In the present study, the binding affinity of naturally occurring alkaloids, hordenine, vincamine, tryptamine, cinchonine, and colcemid was measured with PDK3. The molecular docking and fluorescence binding studies suggested that all these compounds show a considerable binding affinity for PDK3. Read More

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http://dx.doi.org/10.3390/biomedicines8050119DOI Listing

Scorpion Venom: Detriments and Benefits.

Biomedicines 2020 May 12;8(5). Epub 2020 May 12.

Department of Biotechnology and Biomedicine, Technical University of Denmark, DK-2800 Kongens Lyngby, Denmark.

Scorpion venom may cause severe medical complications and untimely death if injected into the human body. Neurotoxins are the main components of scorpion venom that are known to be responsible for the pathological manifestations of envenoming. Besides neurotoxins, a wide range of other bioactive molecules can be found in scorpion venoms. Read More

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http://dx.doi.org/10.3390/biomedicines8050118DOI Listing

Phytogenic Synthesis of Nickel Oxide Nanoparticles (NiO) Using Fresh Leaves Extract of (Wall.) and Investigation of Its Multiple In Vitro Biological Potentials.

Biomedicines 2020 May 12;8(5). Epub 2020 May 12.

Department of Agricultural Sciences, University of Naples Federico II, 80055 Portici, Italy.

Chemically nickel oxide nanoparticles (NiONPs) involve the synthesis of toxic products, which restrict their biological applications. Hence, we developed a simple, eco-friendly, and cost-efficient green chemistry method for the fabrication of NiONPs using fresh leaf broth of (RT). The RT leaves broth was used as a strong reducing, capping, and stabilizing agent in the formation of RT-NiONPs. Read More

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http://dx.doi.org/10.3390/biomedicines8050117DOI Listing

Abnormal Hypermethylation of CpG Dinucleotides in Promoter Regions of Matrix Metalloproteinases Genes in Breast Cancer and Its Relation to Epigenomic Subtypes and HER2 Overexpression.

Biomedicines 2020 May 10;8(5). Epub 2020 May 10.

Epigenetics Laboratory, Research Centre for Medical Genetics, Moskvorechie St 1, 115522 Moscow, Russia.

Matrix metalloproteinases (MMPs) and their tissue inhibitors (TIMPs) substantially contribute to the regulation of intercellular interactions and thereby play a role in maintaining the tissue structure and function. We examined methylation of a subset of 5'-cytosine-phosphate-guanine-3' (CpG) dinucleotides in promoter regions of the , , and genes by methylation-sensitive restriction enzyme digestion PCR. In our collection of 183 breast cancer samples, abnormal hypermethylation was observed for CpGs in , and promoter regions. Read More

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http://dx.doi.org/10.3390/biomedicines8050116DOI Listing

Chronic Periodontitis and Immunity, Towards the Implementation of a Personalized Medicine: A Translational Research on Gene Single Nucleotide Polymorphisms (SNPs) Linked to Chronic Oral Dysbiosis in 96 Caucasian Patients.

Biomedicines 2020 May 9;8(5). Epub 2020 May 9.

Department of Biosciences, Biotechnologies and Biopharmaceutics, Campus Universitario "Ernesto Quagliariello", University of Bari "Aldo Moro", 70125 Bari, Italy.

Chronic periodontitis (CP) is a complex pathology with a significant impact worldwide causing bone loss. Oral dysbiosis is a highly inflammatory condition associated to a long-term insulting infection and represents an underestimated CP key factor associated with an imbalance of pro-inflammatory and anti-inflammatory gene responses. The presence of a single nucleotide polymorphisms (SNPs) in the promoter region of interleukin 10 (IL-10) gene-1082, -819, and -592 was a possible determinant cause. Read More

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http://dx.doi.org/10.3390/biomedicines8050115DOI Listing

RNA Sequencing in Comparison to Immunohistochemistry for Measuring Cancer Biomarkers in Breast Cancer and Lung Cancer Specimens.

Biomedicines 2020 May 9;8(5). Epub 2020 May 9.

Institute of Personalized Medicine, I.M. Sechenov First Moscow State Medical University, Moscow 119048, Russia.

RNA sequencing is considered the gold standard for high-throughput profiling of gene expression at the transcriptional level. Its increasing importance in cancer research and molecular diagnostics is reflected in the growing number of its mentions in scientific literature and clinical trial reports. However, the use of different reagents and protocols for RNA sequencing often produces incompatible results. Read More

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http://dx.doi.org/10.3390/biomedicines8050114DOI Listing

Increased ACh-Associated Immunoreactivity in Autonomic Centers in PTZ Kindling Model of Epilepsy.

Biomedicines 2020 May 8;8(5). Epub 2020 May 8.

Neuropharmacology Research Strength, Jeffrey Cheah School of Medicine and Health Sciences, Monash University Malaysia, Bandar Sunway 47500, Selangor, Malaysia.

Experimental and clinical studies of cardiac pathology associated with epilepsy have demonstrated an impact on the autonomic nervous system (ANS). However, the underlying molecular mechanism has not been fully elucidated. Molecular investigation of the neurotransmitters related receptor and ion channel directing ANS might help in understanding the associated mechanism. Read More

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http://dx.doi.org/10.3390/biomedicines8050113DOI Listing

Mesenchymal Stem Cells: A Trump Card for the Treatment of Diabetes?

Biomedicines 2020 May 6;8(5). Epub 2020 May 6.

Experimental Neurology Unit and Milan Center for Neuroscience, School of Medicine and Surgery, University of Milano-Bicocca, Via Cadore 48, 20900 Monza, Italy.

The advent of the new revolutionary approach based on regenerative medicine is progressively reshaping the therapeutic scenario of many different diseases, such as cardiovascular diseases and immune diseases, with encouraging results. During the last 10 years, many studies have also proposed the use of mesenchymal stem cells (MSCs), adult stem cells with several interesting properties already used in different experimental models, for the treatment of diabetes, however, reporting conflicting outcomes. These reasons have given rise to a question: are these cells a real trump card for the biomedical field? Are they really able to outclass the traditional therapies, or at least able to give an advantage over them? In this review, we will discuss the most promising results obtained with MSCs for the treatment of diabetes and its complications, we will compare the different therapeutic treatments applied as well as the most likely mechanisms of action, and overall we will give an in-depth overview of the pros and the cons of the use of MSCs for the therapy of both type-1 and type-2 diabetes. Read More

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http://dx.doi.org/10.3390/biomedicines8050112DOI Listing

: Identification of the Anti-Inflammatory and Antinociceptive Effects.

Biomedicines 2020 May 6;8(5). Epub 2020 May 6.

Universidade Federal do Rio de Janeiro, Instituto de Ciências Biomédicas, Laboratório de Farmacologia da Dor e da Inflamação, Rio de Janeiro 21941-902, Brazil.

, popularly known as "mil-homens," is widely used for treatment of stomach aches, colic, asthma, pulmonary diseases, diabetes, and skin affection. We evaluated the antinociceptive and anti-inflammatory activities of the essential oil (EO) and the main constituent, 6-methyl-5-hepten-2-yl acetate (sulcatyl acetate, SA). EO and SA (1, 10, and 100 mg/kg, p. Read More

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http://dx.doi.org/10.3390/biomedicines8050111DOI Listing

Nobiletin in Cancer Therapy: How This Plant Derived-Natural Compound Targets Various Oncogene and Onco-Suppressor Pathways.

Biomedicines 2020 May 5;8(5). Epub 2020 May 5.

Amity Institute of Molecular Medicine and Stem Cell Research (AIMMSCR), Amity University Uttar Pradesh, Sector-125, Noida-201313, India.

Cancer therapy is a growing field, and annually, a high number of research is performed to develop novel antitumor drugs. Attempts to find new antitumor drugs continue, since cancer cells are able to acquire resistance to conventional drugs. Natural chemicals can be considered as promising candidates in the field of cancer therapy due to their multiple-targeting capability. Read More

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http://dx.doi.org/10.3390/biomedicines8050110DOI Listing

Coronavirus Pandemic-Therapy and Vaccines.

Biomedicines 2020 May 3;8(5). Epub 2020 May 3.

PanTherapeutics, CH1095 Lutry, Switzerland.

The current coronavirus COVID-19 pandemic, which originated in Wuhan, China, has raised significant social, psychological and economic concerns in addition to direct medical issues. The rapid spread of severe acute respiratory syndrome-coronavirus (SARS-CoV)-2 to almost every country on the globe and the failure to contain the infections have contributed to fear and panic worldwide. The lack of available and efficient antiviral drugs or vaccines has further worsened the situation. Read More

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http://dx.doi.org/10.3390/biomedicines8050109DOI Listing

Can the Molar Insulin: C-Peptide Ratio Be Used to Predict Hyperinsulinaemia?

Biomedicines 2020 May 3;8(5). Epub 2020 May 3.

School of Public Health and Interdisciplinary Studies, Faculty of Health and Environmental Sciences, Auckland University of Technology, Auckland 0627, New Zealand.

Hyperinsulinaemia is the precursor to numerous metabolic disorders. Early diagnosis and intervention could improve population health. Diagnosing hyperinsulinaemia is problematic because insulin has a very short half-life (2-5minutes). Read More

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http://dx.doi.org/10.3390/biomedicines8050108DOI Listing

Structure-Activity Analysis and Molecular Docking Studies of Coumarins from as Multifunctional Agents for Alzheimer's Disease.

Biomedicines 2020 May 2;8(5). Epub 2020 May 2.

Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen 40002, Thailand.

Coumarins, naturally occurring phytochemicals, display a wide spectrum of biological activities by acting on multiple targets. Herein, nine coumarins from the root of were evaluated for activities related to pathogenesis of Alzheimer's disease (AD). They were examined for acetylcholinesterase (AChE) and AChE- or self-induced amyloid beta (Aβ) aggregation inhibitory activities, as well as neuroprotection against HO- and Aβ-induced human neuroblastoma SH-SY5Y cell damage. Read More

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http://dx.doi.org/10.3390/biomedicines8050107DOI Listing

Efficaciousness of Low Affinity Compared to High Affinity TSPO Ligands in the Inhibition of Hypoxic Mitochondrial Cellular Damage Induced by Cobalt Chloride in Human Lung H1299 Cells.

Biomedicines 2020 May 2;8(5). Epub 2020 May 2.

The Ruth and Bruce Rappaport Faculty of Medicine, Technion Institute of Technology, Haifa 31096, Israel.

The 18 kDa translocator protein (TSPO) plays an important role in apoptotic cell death, including apoptosis induced by the hypoxia mimicking agent cobalt chloride (CoCl). In this study, the protective effects of a high (CB86; Ki= 1.6 nM) and a low (CB204; Ki= 117. Read More

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http://dx.doi.org/10.3390/biomedicines8050106DOI Listing

Preparation of Terpenoid-Invasomes with Selective Activity against and Characterization by Cryo Transmission Electron Microscopy.

Biomedicines 2020 May 1;8(5). Epub 2020 May 1.

Thin Films & Physics of Nanostructures, Bielefeld University, Universitätsstrasse 25, 33615 Bielefeld, Germany.

Terpenoids are natural plant-derived products that are applied to treat a broad range of human diseases, such as airway infections and inflammation. However, pharmaceutical applications of terpenoids against bacterial infection remain challenging due to their poor water solubility. Here, we produce invasomes encapsulating thymol, menthol, camphor and 1,8-cineol, characterize them via cryo transmission electron microscopy and assess their bactericidal properties. Read More

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http://dx.doi.org/10.3390/biomedicines8050105DOI Listing

Standardized Extract Reverses Streptozotocin-induced Alzheimer's Disease-Like Condition in a Rat Model.

Biomedicines 2020 Apr 30;8(5). Epub 2020 Apr 30.

Neuropharmacology Research Strength, Jeffrey Cheah School of Medicine and Health Sciences, Monash University Malaysia, Bandar Sunway, Selangor 47500, Malaysia.

Alzheimer's disease (AD) is a chronic neurodegenerative brain disease that is characterized by impairment in cognitive functioning as well as the presence of intraneuronal neurofibrillary tangles (NFTs) and extracellular senile plaques. There is a growing interest in the potential of phytochemicals to improve memory, learning, and general cognitive abilities. The Malaysian herb is a traditional remedy that possesses anti-inflammatory, anti-oxidant, and free-radical scavenging abilities, all of which are known to protect against AD. Read More

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http://dx.doi.org/10.3390/biomedicines8050104DOI Listing

Garcinol Exhibits Anti-Neoplastic Effects by Targeting Diverse Oncogenic Factors in Tumor Cells.

Biomedicines 2020 Apr 30;8(5). Epub 2020 Apr 30.

Department of Science in Korean Medicine, College of Korean Medicine, Kyung Hee University, 24 Kyungheedae-ro, Dongdaemun-gu, Seoul 02447, Korea.

Garcinol, a polyisoprenylated benzophenone, is the medicinal component obtained from fruits and leaves of () and has traditionally been extensively used for its antioxidant and anti-inflammatory properties. In addition, it has been also been experimentally illustrated to elicit anti-cancer properties. Several in vitro and in vivo studies have illustrated the potential therapeutic efficiency of garcinol in management of different malignancies. Read More

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http://dx.doi.org/10.3390/biomedicines8050103DOI Listing

The Role of IL-6 in Skin Fibrosis and Cutaneous Wound Healing.

Biomedicines 2020 Apr 30;8(5). Epub 2020 Apr 30.

School of Biomedical Sciences, University of Western Australia, Crawley, WA 6009, Australia.

The timely resolution of wound healing is critical for restoring the skin as a protective barrier. The switch from a proinflammatory to a reparative microenvironment must be tightly regulated. Interleukin (IL)-6 is a key modulator of the inflammatory and reparative process: it is involved in the differentiation, activation, and proliferation of leukocytes, endothelial cells, keratinocytes, and fibroblasts. Read More

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http://dx.doi.org/10.3390/biomedicines8050101DOI Listing

Pressurized Intraperitoneal Aerosol Chemotherapy (PIPAC) with Oxaliplatin, Cisplatin, and Doxorubicin in Patients with Peritoneal Carcinomatosis: An Open-Label, Single-Arm, Phase II Clinical Trial.

Biomedicines 2020 Apr 30;8(5). Epub 2020 Apr 30.

Candiolo Cancer Institute, Unit of Surgical Oncology, FPO-IRCCS, 10060 Candiolo, Italy.

Pressurized intraperitoneal aerosol chemotherapy (PIPAC) is an innovative approach for treating peritoneal carcinomatosis that applies chemotherapeutic drugs into the peritoneal cavity as an under-pressure airflow. It improves local bioavailability of cytostatic drugs as compared to conventional intraperitoneal chemotherapy. The aim of this study is to prove feasibility, efficacy and safety of this new treatment. Read More

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http://dx.doi.org/10.3390/biomedicines8050102DOI Listing

A Novel Method to Identify Autoantibodies against Putative Target Proteins in Serum from beta-Thalassemia Major: A Pilot Study.

Biomedicines 2020 Apr 26;8(5). Epub 2020 Apr 26.

School of Medicine, International Medical University, Bukit Jalil, Kuala Lumpur 57000, Malaysia.

Abnormal immune reactivity in patients with beta-thalassemia (beta-thal) major can be associated with poor prognosis. Immunome protein-array analysis represents a powerful approach to identify novel biomarkers. The Sengenics Immunome Protein Array platform was used for high-throughput quantification of autoantibodies in 12 serum samples collected from nine beta-thal major patients and three non-thalassemia controls, which were run together with two pooled normal sera (Sengenics Internal QC samples). Read More

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http://dx.doi.org/10.3390/biomedicines8050097DOI Listing

The Minimal Effect of Linker Length for Fatty Acid Conjugation to a Small Protein on the Serum Half-Life Extension.

Biomedicines 2020 Apr 26;8(5). Epub 2020 Apr 26.

School of Materials Science and Engineering, Gwangju Institute of Science and Technology (GIST), Gwangju 61005, Korea.

Conjugation of serum albumin or one of its ligands (such as fatty acid) has been an effective strategy to prolong the serum half-lives of drugs via neonatal Fc receptor (FcRn)-mediated recycling of albumin. So far, fatty acid (FA) has been effective in prolonging the serum half-lives for therapeutic peptides and small proteins, but not for large therapeutic proteins. Very recently, it was reported a large protein conjugated to FA competes with the binding of FcRn with serum albumin, leading to limited serum half-life extension, because primary FA binding sites in serum albumin partially overlap with FcRn binding sites. Read More

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http://dx.doi.org/10.3390/biomedicines8050096DOI Listing

Organic Acids from Roselle ( L.)-A Brief Review of Its Pharmacological Effects.

Biomedicines 2020 Apr 28;8(5). Epub 2020 Apr 28.

Escuela Superior de Medicina, Instituto Politécnico Nacional, "Unidad Casco de Santo Tomas", Ciudad de México 11340, Mexico.

Roselle ( L.), also known as jamaica in Spanish, is a perennial plant that grows in tropical and subtropical regions, including China, Egypt, Indonesia, Mexico, Nigeria, Thailand, and Saudi Arabia. It has a long history of uses, mainly focused on culinary, botanical, floral, cosmetic, and medicinal uses. Read More

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http://dx.doi.org/10.3390/biomedicines8050100DOI Listing