44,408 results match your criteria Biomedicine & Pharmacotherapy[Journal]


Novel ANO5 mutation c.1067G>T (p.C356F) identified by whole genome sequencing in a big family with atypical gnathodiaphyseal dysplasia.

Head Neck 2018 Dec 15. Epub 2018 Dec 15.

Department of Oral and Maxillofacial Surgery, Sun Yat-Sen Memorial Hospital of Sun Yat-Sen University, Guangzhou, China.

Background: Gnathodiaphyseal dysplasia (GDD) is a rare skeletal disorder that has not been well studied.

Methods: Sanger sequencing, whole-genome sequencing (WGS), and bioinformatics and structural modeling analyses were performed.

Results: A family with patients with fibro-osseous lesions of the jawbones were initially diagnosed with cherubism. Read More

View Article
December 2018

Orf virus infection of human keratinocytes and dermal fibroblasts: limited virus detection and interference with intercellular adhesion molecule-1 up-regulation.

Exp Dermatol 2018 Dec 15. Epub 2018 Dec 15.

Institute of Immunology/Molecular Pathogenesis, Center for Biotechnology and Biomedicine, College of Veterinary Medicine, Leipzig University, Leipzig, Germany.

Orf virus (Parapoxvirus ovis, ORFV) is a dermatotropic virus causing pustular dermatitis in small ruminants and humans. We analyzed isolated human primary keratinocytes (KC) and dermal fibroblasts (FB) for cell death and virus replication by infection with a patient-derived ORFV isolate. ORFV infection was associated with rapid induction of cell death in KC allowing for considerable virus removal. Read More

View Article
December 2018
1 Read

Plasma circulating tumor DNA as a potential tool for disease monitoring in head and neck cancer.

Head Neck 2018 Dec 15. Epub 2018 Dec 15.

Department of Surgery, Boston Medical Center, Boston University School of Medicine, Boston, MA, USA.

Background: Recommendations for perioperative therapy in head and neck cancer are not explicit and recurrence occurs frequently. Circulating tumor DNA is an emerging cancer biomarker, but has not been extensively explored for detection of recurrence in head and neck cancer.

Methods: Patients diagnosed with head and neck squamous cell carcinoma were recruited into the study protocol. Read More

View Article
December 2018

Manilkara zapota (L.) P. Royen leaf water extract triggered apoptosis and activated caspase-dependent pathway in HT-29 human colorectal cancer cell line.

Biomed Pharmacother 2018 Dec 13;110:748-757. Epub 2018 Dec 13.

Department of Nutrition and Dietetics, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia; Research Centre of Excellent, Nutrition and Non-Communicable Diseases (NNCD), Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia; Laboratory of Molecular Biomedicine, Institute of Bioscience, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia. Electronic address:

Manilkara zapota (L.) P. Royen (Family: Sapotaceae), commonly called as sapodilla, has been applied as traditional folk medicine for diarrhea and pulmonary infections. Read More

View Article
December 2018

Utility of sheathless capillary electrophoresis-mass spectrometry for metabolic profiling of limited sample amounts.

J Chromatogr B Analyt Technol Biomed Life Sci 2018 Dec 6;1105:10-14. Epub 2018 Dec 6.

Biomedical Microscale Analytics, Division of Systems Biomedicine and Pharmacology, Leiden Academic Center for Drug Research, Leiden University, the Netherlands. Electronic address:

Metabolomics studies using a small amount of cells may save time and money, while in some cases (e.g., profiling pathogenic cells in an early-stage tissue), only a small number of cells are accessible for analysis. Read More

View Article
December 2018

Intranasal insulin activates Akt2 signaling pathway in the hippocampus of wild-type but not in APP/PS1 Alzheimer model mice.

Neurobiol Aging 2018 Nov 17;75:98-108. Epub 2018 Nov 17.

Institute of Biomedicine, University of Eastern Finland, Kuopio, Finland. Electronic address:

Type 2 diabetes mellitus (T2DM) increases the risk for Alzheimer's disease (AD). Human AD brains show reduced glucose metabolism as measured by [18F]fluoro-2-deoxy-2-D-glucose positron emission tomography (FDG-PET). Here, we used 14-month-old wild-type (WT) and APP/PS1 (APP/PS1) transgenic mice to investigate how a single dose of intranasal insulin modulates brain glucose metabolism using FDG-PET and affects spatial learning and memory. Read More

View Article
November 2018

Imprinted genes influencing the quality of maternal care.

Front Neuroendocrinol 2018 Dec 13. Epub 2018 Dec 13.

Biomedicine Division, School of Biosciences, Cardiff University, Cardiff, UK, CF10 3AX, UK. Electronic address:

In mammals successful rearing imposes a cost on later reproductive fitness specifically on the mother creating the potential for parental conflict. Loss of function of three imprinted genes in the dam result in deficits in maternal care suggesting that, like maternal nutrients, maternal care is a resource over which the parental genomes are in conflict. However, the induction of maternal care is a complex and highly regulated process. Read More

View Article
December 2018

Sinus Venosus Defects: Anatomic Variants and Transcatheter Closure Feasibility Using Virtual Reality Planning.

JACC Cardiovasc Imaging 2018 Dec 6. Epub 2018 Dec 6.

Department of Pediatrics (Cardiology), University of Texas Southwestern Medical Center, Children's Medical Center Dallas, Dallas, Texas; Department of Radiology, University of Texas Southwestern Medical Center, Children's Medical Center Dallas, Dallas, Texas.

View Article
December 2018

An overview of deep learning in medical imaging focusing on MRI.

Z Med Phys 2018 Dec 12. Epub 2018 Dec 12.

Mohn Medical Imaging and Visualization Centre (MMIV), Haukeland University Hospital, Norway; Neuroinformatics and Image Analysis Laboratory, Department of Biomedicine, University of Bergen, Norway; Department of Health and Functioning, Western Norway University of Applied Sciences, Norway. Electronic address:

What has happened in machine learning lately, and what does it mean for the future of medical image analysis? Machine learning has witnessed a tremendous amount of attention over the last few years. The current boom started around 2009 when so-called deep artificial neural networks began outperforming other established models on a number of important benchmarks. Deep neural networks are now the state-of-the-art machine learning models across a variety of areas, from image analysis to natural language processing, and widely deployed in academia and industry. Read More

View Article
December 2018

Modification of chitosan grafted with collagen peptide by enzyme crosslinking.

Carbohydr Polym 2019 Feb 16;206:468-475. Epub 2018 Oct 16.

The State Key Laboratory Breeding Base of Basic Science of Stomatology, Hubei Province & Key Laboratory of Oral Biomedicine (Wuhan University), Ministry of Education, Luoyu Road 237, Wuhan 430079, Hubei, China. Electronic address:

Free radicals are closely related to the occurrence and development of aging, cancer and inflammation. In this paper, the microbial transglutaminase (MTGase) was used as a catalyst to graft the collagen peptide (COP) molecules on the amino group of carboxymethyl chitosan sulfate (CMCS) to improve the antioxidant effects. FT-IR and NMR spectroscopy were used to confirm the successful grafting of COP to CMCS. Read More

View Article
February 2019

Anti-inflammatory and antioxidant nanostructured cellulose membranes loaded with phenolic-based ionic liquids for cutaneous application.

Carbohydr Polym 2019 Feb 25;206:187-197. Epub 2018 Oct 25.

CICECO, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, Portugal. Electronic address:

The utilization of natural compounds, such as phenolic acids and biopolymers, in the healthcare domain is gaining increasing attention. In this study, bacterial nanocellulose (BC) membranes were loaded with ionic liquids (ILs) based on phenolic acids. These ionic compounds, with improved solubility and bioavailability, were prepared by combining the cholinium cation with anions derived from caffeic, ellagic and gallic acids. Read More

View Article
February 2019
1 Read

Janus multi-responsive superparamagnetic nanoparticles functionalized with two on-demand and independently cleavable ligands for Actinide separation.

J Colloid Interface Sci 2018 Dec 7;538:546-558. Epub 2018 Dec 7.

State Key Laboratory of Environment-friendly Energy Materials, Southwest University of Science and Technology, Mianyang 621010, China. Electronic address:

Hypothesis: Nanoparticles functionalized with ligands which can on-demand and remotely be detached have recently attracted interest as stimuli-responsive materials. Research is now focused on multi-responsive systems, with applications in environmental science and biomedicine. The possibility to covalently couple two different ligands on a single nanoparticles, and to release them independently is investigated. Read More

View Article
December 2018
1 Read

Double mild stimulation and egg collection in the same cycle for management of poor ovarian responders.

J Gynecol Obstet Hum Reprod 2018 Dec 12. Epub 2018 Dec 12.

Department of Epidemiology and Reproductive Health, Reproductive Epidemiology Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran. Electronic address:

Purpose: To evaluate the effect of double stimulations during the follicular and luteal phases in women with poor ovarian response (POR) in in-vitro fertilization/intracytoplasmic sperm injection (IVF/ICSI) cycles.

Basic Procedures: This prospective clinical study was performed in Royan Institute from October 2014 to January 2016. 121 patients were diagnosed as POR on the basis of Bologna criteria were included. Read More

View Article
December 2018

Immune cells in the retina and choroid: Two different tissue environments that require different defenses and surveillance.

Prog Retin Eye Res 2018 Dec 12. Epub 2018 Dec 12.

Department of Anatomy and Developmental Biology, Monash Biomedicine Discovery Institute, Monash University, Clayton, Victoria, Australia.

In the eye immune defenses must take place in a plethora of differing microenvironments ranging from the corneal and conjunctival epithelia facing the external environment to the pigmented connective tissue of the uveal tract containing smooth muscle, blood vessels and peripheral nerves to the innermost and highly protected neural retina. The extravascular environment of the neural retina, like the brain parenchyma, is stringently controlled to maintain conditions required for neural transmission. The unique physiological nature of the neural retina can be attributed to the blood retinal barriers (BRB) of the retinal vasculature and the retinal pigment epithelium, which both tightly regulate the transport of small molecules and restrict passage of cells and macromolecules from the circulation into the retina in a similar fashion to the blood brain barrier (BBB). Read More

View Article
December 2018

Biogenic nanomaterials: Synthesis, characterization, growth mechanism, and biomedical applications.

J Microbiol Methods 2018 Dec 12. Epub 2018 Dec 12.

School of Pharmacy, Sungkyunkwan University, 2066 Seoburo, Jangan-gu, Suwon, Gyeonggido 16419, Republic of Korea. Electronic address:

The biosynthesis of nanomaterials is a huge and intensifying field of research due to their application in various areas, in particular the biomedical and pharmaceutical fields. In this review, we focused on the biosynthesis of both metallic and semiconductor nanomaterials and their application in biomedicine and pharmaceutics. In order to meet an exponentially increasing need for nanostructured materials, the biological route for the synthesis of nanomaterials will have to be explored, offering advantages over chemical and physical methods as a simpler, more cost effective, and environmentally friendly method, and for which there is no need to use high pressure and temperatures or toxic chemicals. Read More

View Article
December 2018

Non-covalent hitchhiking on endogenous carriers as a protraction mechanism for antiviral macromolecular prodrugs.

J Control Release 2018 Dec 12. Epub 2018 Dec 12.

Department of Chemistry, Aarhus University, 8000 Aarhus C, Denmark. Electronic address:

Albumin is a highly successful tool of drug delivery providing drastically extended body and blood residence time for the associated cargo, but it only traffics single drug copies at a time. In turn, macromolecular prodrugs (MP) are advantaged in carrying a high drug payload but offering only a modest extension of residence time to the conjugated drugs. In this work, we engineer MP to contain terminal groups that bind to albumin via non-covalent association and reveal that this facile measure affords a significant protraction for the associated polymers. Read More

View Article
December 2018

Improving the quality and workflow of bacterial genome sequencing and analysis: paving the way for a Switzerland-wide molecular epidemiological surveillance platform.

Swiss Med Wkly 2018 Dec 15;148:w14693. Epub 2018 Dec 15.

SIB Swiss Institute of Bioinformatics, Lausanne, Switzerland.

Facing multidrug resistant (MDR) bacterial pathogens is one of the most important challenges for our society. The spread of highly virulent and resistant pathogens can be described using molecular typing technologies; in particular, whole genome sequencing (WGS) data can be used for molecular typing purposes with high resolution. WGS data analysis can explain the spatiotemporal patterns of pathogen transmission. Read More

View Article
December 2018
1 Read

Scutellarein inhibits RANKL-induced osteoclast formation in vitro and prevents LPS-induced bone loss in vivo.

J Cell Physiol 2018 Dec 14. Epub 2018 Dec 14.

Department of Trauma Orthopedic and Hand Surgery, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, China.

Osteoporosis, arthritis, Peget's disease, bone tumor, periprosthetic joint infection, and periprosthetic loosening have a common characteristic of osteolysis, which is characterized by the enhanced osteoclastic bone resorptive function. At present, the treatment target of these diseases is to interfere with osteoclastic formation and function. Scutellarein (Scu), a flavonoids compound, can inhibit the progress of tumor and inflammation. Read More

View Article
December 2018

Minimal residual disease quantification by flow cytometry provides reliable risk stratification in T-cell acute lymphoblastic leukemia.

Leukemia 2018 Dec 14. Epub 2018 Dec 14.

Department of Clinical Immunology, Copenhagen University Hospital Rigshospitalet, Copenhagen, Denmark.

Minimal residual disease (MRD) measured by PCR of clonal IgH/TCR rearrangements predicts relapse in T-cell acute lymphoblastic leukemia (T-ALL) and serves as risk stratification tool. Since 10% of patients have no suitable PCR-marker, we evaluated flowcytometry (FCM)-based MRD for risk stratification. We included 274 T-ALL patients treated in the NOPHO-ALL2008 protocol. Read More

View Article
December 2018

Successful Anti-PD-1 Cancer Immunotherapy Requires T Cell-Dendritic Cell Crosstalk Involving the Cytokines IFN-γ and IL-12.

Immunity 2018 Nov 27. Epub 2018 Nov 27.

Center for Systems Biology, Massachusetts General Hospital, 185 Cambridge St, CPZN 5206, Boston, MA 02114, USA; Department of Radiology, Massachusetts General Hospital, 185 Cambridge St, CPZN 5206, Boston, MA 02114, USA. Electronic address:

Anti-PD-1 immune checkpoint blockers can induce sustained clinical responses in cancer but how they function in vivo remains incompletely understood. Here, we combined intravital real-time imaging with single-cell RNA sequencing analysis and mouse models to uncover anti-PD-1 pharmacodynamics directly within tumors. We showed that effective antitumor responses required a subset of tumor-infiltrating dendritic cells (DCs), which produced interleukin 12 (IL-12). Read More

View Article
November 2018

Simultaneous Loss of Both Atypical Protein Kinase C Genes in the Intestinal Epithelium Drives Serrated Intestinal Cancer by Impairing Immunosurveillance.

Immunity 2018 Dec 1. Epub 2018 Dec 1.

Cancer Metabolism and Signaling Networks Program, Sanford Burnham Prebys Medical Discovery Institute, La Jolla, CA 92037, USA. Electronic address:

Serrated adenocarcinoma, an alternative pathway for colorectal cancer (CRC) development, accounts for 15%-30% of all CRCs and is aggressive and treatment resistant. We show that the expression of atypical protein kinase C ζ (PKCζ) and PKCλ/ι was reduced in human serrated tumors. Simultaneous inactivation of the encoding genes in the mouse intestinal epithelium resulted in spontaneous serrated tumorigenesis that progressed to advanced cancer with a strongly reactive and immunosuppressive stroma. Read More

View Article
December 2018

Axoglial Adhesion by Cadm4 Regulates CNS Myelination.

Neuron 2018 Dec 5. Epub 2018 Dec 5.

Department of Molecular Cell Biology, The Weizmann Institute of Science, Rehovot 76100, Israel. Electronic address:

The initiation of axoglial contact is considered a prerequisite for myelination, yet the role cell adhesion molecules (CAMs) play in mediating such interactions remains unclear. To examine the function of axoglial CAMs, we tested whether enhanced CAM-mediated adhesion between OLs and neurons could affect myelination. Here we show that increased expression of a membrane-bound extracellular domain of Cadm4 (Cadm4dCT) in cultured oligodendrocytes results in the production of numerous axoglial contact sites that fail to elongate and generate mature myelin. Read More

View Article
December 2018

Phyto-Mediated Photo Catalysed Green Synthesis of Silver Nanoparticles Using Durio Zibethinus Seed Extract: Antimicrobial and Cytotoxic Activity and Photocatalytic Applications.

Molecules 2018 Dec 13;23(12). Epub 2018 Dec 13.

Faculty of Pharmacy, AIMST University, Semeling 8100, Bedong, Kedah, Malaysia.

In the present study, we have developed a green approach for the synthesis of silver nanoparticles (DSAgNPs) using aqueous extract of seed and determined its antibacterial, photocatalytic and cytotoxic effects. Surface plasmon resonance confirmed the formation of DSAgNPs with a maximum absorbance (λ) of 420 nm. SEM and TEM images revealed DSAgNPs were spherical and rod shaped, with a size range of 20 nm and 75 nm. Read More

View Article
December 2018
1 Read

MS/MS-Based Molecular Networking Approach for the Detection of Aplysiatoxin-Related Compounds in Environmental Marine Cyanobacteria.

Mar Drugs 2018 Dec 13;16(12). Epub 2018 Dec 13.

Natural Sciences and Science Education, National Institute of Education, Nanyang Technological University, Singapore 637616, Singapore.

Certain strains of cyanobacteria produce a wide array of cyanotoxins, such as microcystins, lyngbyatoxins and aplysiatoxins, that are associated with public health issues. In this pilot study, an approach combining LC-MS/MS and molecular networking was employed as a rapid analytical method to detect aplysiatoxins present in four environmental marine cyanobacterial samples collected from intertidal areas in Singapore. Based on 16S-ITS rRNA gene sequences, these filamentous cyanobacterial samples collected from Pulau Hantu were determined as , sp. Read More

View Article
December 2018

Lymphatic Vascular Structures: A New Aspect in Proliferative Diabetic Retinopathy.

Int J Mol Sci 2018 Dec 13;19(12). Epub 2018 Dec 13.

Research Programs Unit, Genome-Scale Biology, Biomedicum Helsinki, University of Helsinki, FI-00014 Helsinki, Finland.

Diabetic retinopathy (DR) is the most common diabetic microvascular complication and major cause of blindness in working-age adults. According to the level of microvascular degeneration and ischemic damage, DR is classified into non-proliferative DR (NPDR), and end-stage, proliferative DR (PDR). Despite advances in the disease etiology and pathogenesis, molecular understanding of end-stage PDR, characterized by ischemia- and inflammation-associated neovascularization and fibrosis, remains incomplete due to the limited availability of ideal clinical samples and experimental research models. Read More

View Article
December 2018
2 Reads

Frailty as the Future Core Business of Public Health: Report of the Activities of the A3 Action Group of the European Innovation Partnership on Active and Healthy Ageing (EIP on AHA).

Int J Environ Res Public Health 2018 Dec 13;15(12). Epub 2018 Dec 13.

Department of Biomedicine and Prevention, University of Rome "Tor Vergata", 00133 Rome, Italy.

The prevalence of frailty at population-level is expected to increase in Europe, changing the focus of Public Health. Here, we report on the activities of the A3 Action Group, focusing on managing frailty and supporting healthy ageing at community level. A three-phased search strategy was used to select papers published between January 2016 and May 2018. Read More

View Article
December 2018

Biosynthetic Oligoclonal Antivenom (BOA) for Snakebite and Next-Generation Treatments for Snakebite Victims.

Toxins (Basel) 2018 Dec 13;10(12). Epub 2018 Dec 13.

Department of Biotechnology and Biomedicine, Technical University of Denmark, DK-2800 Kongens Lyngby, Denmark.

Snakebite envenoming is a neglected tropical disease that each year claims the lives of 80,000⁻140,000 victims worldwide. The only effective treatment against envenoming involves intravenous administration of antivenoms that comprise antibodies that have been isolated from the plasma of immunized animals, typically horses. The drawbacks of such conventional horse-derived antivenoms include their propensity for causing allergenic adverse reactions due to their heterologous and foreign nature, an inability to effectively neutralize toxins in distal tissue, a low content of toxin-neutralizing antibodies, and a complex manufacturing process that is dependent on husbandry and procurement of snake venoms. Read More

View Article
December 2018
1 Read

Effects of 5-Aza on p-Y1472 NR2B related to learning and memory in the mouse hippocampus.

Biomed Pharmacother 2019 Jan 5;109:701-707. Epub 2018 Nov 5.

Inner Mongolia Key Laboratory of Hypoxic Translational Medicine, Baotou Medical College, Inner Mongolia, People's Republic of China; Biomedicine Research Center, Basic Medical College and Baotou Medical College of Neuroscience Institute, Baotou Medical College, Inner Mongolia, People's Republic of China; Beijing Key Laboratory of Hypoxic Conditioning Translational Medicine, Xuanwu Hospital, Capital Medical University, Beijing, People's Republic of China. Electronic address:

Background: We have previously reported that 5-Aza-2-deoxycytidine (5-Aza-cdR) can repress protein serine/threonine phosphatase-1γ (PP1γ) expression and activity in the mouse hippocampus and affect the behaviour of mice in a water maze. It is well known that the phosphorylation of N-methyl-d-aspartate receptor 2B subunit (NR2B) plays a role in behaviour. In this study, we examined whether 5-Aza-cdR affects NR2B phosphorylation at tyrosine 1472 (p-Y1472 NR2B) and whether it affected the responses of the mice in a passive avoidance test. Read More

View Article
January 2019

Cardiac and pulmonary toxicity of mesoporous silica nanoparticles is associated with excessive ROS production and redox imbalance in Wistar rats.

Biomed Pharmacother 2019 Jan 3;109:2527-2538. Epub 2018 Dec 3.

Materials Science and Nanotechnology Department, Faculty of Postgraduate Studies for Advanced Sciences (PSAS), Beni-Suef University, Egypt.

Mesoporous silica nanoparticles (MSNs) represent one of the most promising drug delivery systems. MSNs have attracted considerable attention in recent years both in industry and biomedicine due to their unique properties. Thus, evaluation of the toxic effects of MSNs is necessary before the biomedical and clinical applications. Read More

View Article
January 2019

Mesoporous silica nanoparticles for therapeutic/diagnostic applications.

Biomed Pharmacother 2019 Jan 6;109:1100-1111. Epub 2018 Nov 6.

Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran. Electronic address:

Based on unique intrinsic properties of mesoporous silica nanoparticles (MSNs) such as high surface area, large pore size, good biocompatibility and biodegradability, stable aqueous dispersion, they have received much attention in the recent decades for their applications as a promising platform in the biomedicine field. These porous structures possess a pore size ranging from 2 to 50 nm which make them excellent candidates for various biomedical applications. Herein, at first we described the common approaches of cargo loading and release processes from MSNs. Read More

View Article
January 2019

Chronic fatigue syndrome (CFS): Suggestions for a nutritional treatment in the therapeutic approach.

Biomed Pharmacother 2019 Jan 5;109:1000-1007. Epub 2018 Nov 5.

Research Department, Innlandet Hospital Trust, Brumunddal, Norway; Inland Norway University of Applied Sciences, Elverum, Norway.

Chronic fatigue syndrome (CFS) is known as a multi-systemic and complex illness, which induces fatigue and long-term disability in educational, occupational, social, or personal activities. The diagnosis of this disease is difficult, due to lacking a proper and suited diagnostic laboratory test, besides to its multifaceted symptoms. Numerous factors, including environmental and immunological issues, and a large spectrum of CFS symptoms, have recently been reported. Read More

View Article
January 2019

Is the perception of illusions abnormal in schizophrenia?

Psychiatry Res 2018 Dec 30;270:929-939. Epub 2018 Oct 30.

Laboratory of Psychophysics, Brain Mind Institute, École Polytechnique Fédérale de Lausanne (EPFL), Switzerland.

There seems to be no common factor for visual perception, i.e., performance in visual tasks correlates only weakly with each other. Read More

View Article
December 2018

Sex differences in an old adult sample with substance use disorder: A 6 months follow-up study.

Psychiatry Res 2018 Dec 16;270:1157-1165. Epub 2018 Oct 16.

Addiction and Dual Diagnosis Unit, Vall Hebron University Hospital, Barcelona, Spain; Psychiatry Service, CIBERSAM, Vall Hebron University Hospital, Barcelona, Spain; Autonomous University of Barcelona, Barcelona, Spain.

Substance use disorder (SUD) is a worldwide concern that has its own particularities regarding age and sex. This study aims to assess the differences between old SUD women and men regarding socio-demographics, clinical factors and outcomes. A 6-months follow-up longitudinal study was conducted in an outpatient center, on a convenience sample of 115 SUD old adults (≥65 years old, average age of 71. Read More

View Article
December 2018

Light-induced mechanisms for nanocarrier's cargo release.

Colloids Surf B Biointerfaces 2019 Jan 25;173:825-832. Epub 2018 Oct 25.

Nanobiotechnology for diagnostics group (Nb4D), Department of Chemical and Biomolecular Nanotechnology, Institute for Advanced Chemistry of Catalonia (IQAC) of the Spanish Council for Scientific Research (CSIC), Spain; CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), Jordi Girona 18-26, 08034 Barcelona, Spain.

Nanomaterials have been the focus of attention in several fields, including biomedicine, electronics, or catalysis, mainly due to the novel properties of the materials at the nanoscale. In the field of diagnosis, nanomaterials have been contemplated as an opportunity to improve sensitivity and time of response, therefore, facilitating early treatment and monitoring of the disease. For therapeutic applications, new drug delivery nanosystems aiming to provide enhanced efficiency have been proposed often addressing selective or controlled delivery of therapeutic agents to particular cells to maximize treatment efficacy minimizing adverse effects. Read More

View Article
January 2019
1 Read

CRISPR-C: circularization of genes and chromosome by CRISPR in human cells.

Nucleic Acids Res 2018 Dec;46(22):e131

Department of Biomedicine, Aarhus University, Denmark.

Extrachromosomal circular DNA (eccDNA) and ring chromosomes are genetic alterations found in humans with genetic disorders. However, there is a lack of genetic engineering tools to recapitulate and study the biogenesis of eccDNAs. Here, we created a dual-fluorescence biosensor cassette, which upon the delivery of pairs of CRISPR/Cas9 guide RNAs, CRISPR-C, allows us to study the biogenesis of a specific fluorophore expressing eccDNA in human cells. Read More

View Article
December 2018

Near-infrared light control of bone regeneration with biodegradable photothermal osteoimplant.

Biomaterials 2018 Dec 10;193:1-11. Epub 2018 Dec 10.

Institute of Biomedicine and Biotechnology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China; Department of Physics and Materials Science, City University of Hong Kong, Tat Chee Avenue, Kowloon, Hong Kong, China. Electronic address:

Mild heat stimulation can promote the restoration of bone defects but unfortunately, the delivery of exo-hyperthermy into human body is not efficient enough. In this study, mild heat-induced osteogenesis with high efficacy is demonstrated on an osteoimplant composed of black phosphorus nanosheets and poly(lactic-co-glycolic acid) (BPs@PLGA) with the participation of near-infrared (NIR) light irradiation. BPs@PLGA with only 0. Read More

View Article
December 2018
2 Reads

Modulation of vitamin D signaling by the pioneer factor CEBPA.

Biochim Biophys Acta Gene Regul Mech 2018 Dec 11. Epub 2018 Dec 11.

School of Medicine, Institute of Biomedicine, University of Eastern Finland, FI-70211 Kuopio, Finland. Electronic address:

The myeloid master regulator CCAAT enhancer-binding protein alpha (CEBPA) is known as a pioneer factor. In this study, we report the CEBPA cistrome of THP-1 human monocytes after stimulation with the vitamin D receptor (VDR) ligand 1α,25-dihydroxyvitamin D (1,25(OH)D) for 2, 8 and 24 h. About a third of the genomic VDR binding sites co-located with those of CEBPA. Read More

View Article
December 2018
1 Read
6.332 Impact Factor

LAIR-1 overexpression and correlation with advanced pathological grade and immune suppressive status in oral squamous cell carcinoma.

Head Neck 2018 Dec 14. Epub 2018 Dec 14.

The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory of Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan University, Wuhan, China.

Background: This study aims to investigate the characteristic role of inhibitory receptor leukocyte-associated immunoglobulin-like receptor 1 (LAIR-1) in oral squamous cell carcinoma (OSCC).

Methods: The expressions of LAIR-1 and other immune-related molecules were detected in a human OSCC tissue microarray. LAIR-1 expression difference among different clinicopathological parameters was analyzed. Read More

View Article
December 2018
2 Reads

FLiRT Technique "Fistula Lipocytes Regenerative Therapy"; autologous adipose derived mesenchymal stem cells auto-transplantation to treat complex fistula-in-ano - Video Vignette.

Colorectal Dis 2018 Dec 13. Epub 2018 Dec 13.

Department of General Surgery UOC C, University of Rome Tor Vergata, Rome, Italy.

Various surgical options have been proposed for the treatment of Fistula-in-Ano (FIA). A balance between healing, recurrence, and continence preservation rates should be maintained which appears to be difficult in complex FIA, such as recurrent, high trans-sphincteric, anterior fistula in a female, multiple tracts, and patients with Crohn's disease. This article is protected by copyright. Read More

View Article
December 2018

PREDICTION ALGORITHM OF THE CAT SPINAL SEGMENTS LENGTHS AND POSITIONS IN RELATION TO THE VERTEBRAE.

Anat Rec (Hoboken) 2018 Dec 12. Epub 2018 Dec 12.

Pavlov Institute of Physiology, Russian Academy of Sciences, nab. Makarova 6, Saint-Petersburg, 199034, Russia.

Detailed knowledge of the topographic organization and precise access to the spinal cord segments is crucial for the neurosurgical manipulations as well as in vivo neurophysiological investigations of the spinal networks involved in sensorimotor and visceral functions. Because of high individual variability, accurate identification of particular portion of the lumbosacral enlargement is normally possible only during postmortem dissection. Yet, it is often necessary to determine the precise location of spinal segments prior to in vivo investigation, targeting spinal cord manipulations, neurointerface implantations, and neuronal activity recordings. Read More

View Article
December 2018
1 Read

Multiple imputation approach for interval-censored time to HIV RNA viral rebound within a mixed effects Cox model.

Biom J 2018 Dec 13. Epub 2018 Dec 13.

Departarment d'Estadística i Investigació Operativa, Universitat Politècnica de Catalunya/BARCELONATECH, Barcelona, Spain.

We present a method to fit a mixed effects Cox model with interval-censored data. Our proposal is based on a multiple imputation approach that uses the truncated Weibull distribution to replace the interval-censored data by imputed survival times and then uses established mixed effects Cox methods for right-censored data. Interval-censored data were encountered in a database corresponding to a recompilation of retrospective data from eight analytical treatment interruption (ATI) studies in 158 human immunodeficiency virus (HIV) positive combination antiretroviral treatment (cART) suppressed individuals. Read More

View Article
December 2018

Exploring the binding interaction of the by-product of Maillard reaction 5-hydroxymethyl-2-furaldehyde with calf thymus DNA.

J Sci Food Agric 2018 Dec 12. Epub 2018 Dec 12.

State Key Laboratory of Food Science and Technology, Nanchang University, 235 Nanjing East Road Nanchang, 330047, China.

Background: 5-Hydroxymethyl-2-furaldehyde (5-HMF), a kind of by-product of Maillard reaction, usually existed in fried and baked food, it may cause potential harm to human body. Here, the interaction between 5-HMF and calf thymus DNA (ctDNA) under physiological buffer (pH 7.4) was studied using multispectroscopic methods combined with multivariate curve resolution-alternating least-squares (MCR-ALS) chemometrics and molecular simulation techniques. Read More

View Article
December 2018

Brown adipose tissue thermogenesis in polycystic ovary syndrome.

Clin Endocrinol (Oxf) 2018 Dec 12. Epub 2018 Dec 12.

Department of Physiology, Monash University, Melbourne, Victoria, Australia.

Objective: Polycystic ovary syndrome (PCOS) is associated with increased obesity with a greater propensity to weight gain and a lack of sustainable lifestyle interventions. Altered brown adipose tissue (BAT) thermogenesis is a potential contributor to obesity in PCOS. BAT activity and modulation has not been studied in PCOS. Read More

View Article
December 2018
1 Read

Hyperpolarized [1- C] pyruvate as a possible diagnostic tool in liver disease.

Physiol Rep 2018 Dec;6(23):e13943

MR Research Centre, Aarhus University Hospital, Aarhus, Denmark.

Introduction of hyperpolarized magnetic resonance in preclinical studies and lately translation to patients provides new detailed in vivo information of metabolic flux in organs. Hyperpolarized magnetic resonance based on C enriched pyruvate is performed without ionizing radiation and allows quantification of the pyruvate conversion products: alanine, lactate and bicarbonate in real time. Thus, this methodology has a promising potential for in vivo monitoring of energetic alterations in hepatic diseases. Read More

View Article
December 2018
3 Reads

High- and Ultra-High definition of IR spectral histopathology gives an insight into chemical environment of lung metastases in breast cancer.

J Biophotonics 2018 Dec 12:e201800345. Epub 2018 Dec 12.

Faculty of Chemistry, Jagiellonian University, Poland.

Using high- (HD) and ultra-high-definition (UHD) of Fourier Transform Infrared (FTIR) spectroscopic imaging, we characterised spectrally pulmonary metastases in a murine model of breast cancer comparing them with histopathological results (H&E staining). This comparison showed excellent agreement between the methods in case of localisation of metastases with size below 1 mm and revealed that label-free HD and UHD IR spectral histopathology distinguish the type of neoplastic cells. We primary focused on differentiation between metastatic foci in the pleural cavity from cancer cells present in lung parenchyma and inflamed cells present in extracellular matrix of lungs due to growing of advanced metastases. Read More

View Article
December 2018

TREM2 triggers microglial density and age-related neuronal loss.

Glia 2018 Dec 11. Epub 2018 Dec 11.

Neural Regeneration, Institute of Reconstructive Neurobiology, University Hospital of Bonn, University of Bonn, Bonn, Germany.

The microglial triggering receptor expressed on myeloid cells 2 (TREM2) signals via the activatory membrane adaptor molecule TYROBP. Genetic variants or mutations of TREM2 or TYROBP have been linked to inflammatory neurodegenerative diseases associated with aging. The typical aging process goes along with microglial changes and mild neuronal loss, but the exact contribution of TREM2 is still unclear. Read More

View Article
December 2018

TIGD1, a gene of unknown function, involves cell-cycle progression and correlates with poor prognosis in human cancer.

J Cell Biochem 2018 Dec 11. Epub 2018 Dec 11.

Laboratory of Tropical Biomedicine and Biotechnology, School of Tropical Medicine and Laboratory Medicine, Hainan Medical University, Haikou, China.

Background: Trigger transposable element-derived 1 (TIGD1), a human species-specific gene, was identified as a cell cycle-related differentially expressed gene during the formation of liver cancer. As far as we know, there are no reports about the function of TIGD1. This study aimed to explore the expression of TIGD1 in human cancers and establish whether elevated TIGD1 can be used as a prognostic cancer biomarker and its potential role in cancer. Read More

View Article
December 2018

MiRNAs-181a/b therapy in lung cancer: reality or myth?

Mol Oncol 2018 Dec 8. Epub 2018 Dec 8.

Research Center for Functional Genomics, Biomedicine and Translational Medicine, Iuliu Hatieganu" University of Medicine and Pharmacy, Cluj-Napoca, Romania.

Despite substantial progress in oncology, lung cancer remains the number one malignancy in terms of both incidence and mortality rates, and there thus remains an urgent need for new therapeutic alternatives. MicroRNAs (miRNAs) have an important role in cancer initiation and progression due to their capacity to interfere with transcriptional signaling and regulate key cellular processes. miR-181a and miR-181b (miR-181a/b), which are located on chromosomes 1 and 9, are pathologically expressed in the tumor tissue and plasma of patients diagnosed with lung cancer. Read More

View Article
December 2018

Obstetric and perinatal outcomes of singleton pregnancies conceived via assisted reproductive technology complicated by gestational diabetes mellitus: a prospective cohort study.

BMC Pregnancy Childbirth 2018 Dec 14;18(1):495. Epub 2018 Dec 14.

Endocrine Research Center, Institute of Endocrinology and Metabolism, Iran University of Medical Sciences (IUMS), Firouzeh St., South Vali- Asr Ave., Vali- Asr Sq., Tehran, 15937-16615, Iran.

Background: Growing evidence indicates that the risk of obstetric and perinatal outcomes is higher in women with assisted reproductive technology (ART). However, there is little known about pregnancy related complications and co-morbidity in gestational diabetes mellitus (GDM) following singleton pregnancies achieved by ART in comparison with spontaneous conception (SC).

Methods: Two hundred sixty singleton pregnant women conceived by ART and 314 pregnant women conceived by spontaneous conception (SC) were participated in this prospective cohort study. Read More

View Article
December 2018
1 Read

Drug-Induced Expression-Based Computational Repurposing of Small Molecules Affecting Transcription Factor Activity.

Methods Mol Biol 2019 ;1903:179-184

Department of Physiology and Biophysics, Institute for Computational Biomedicine, Weill Cornell Medicine, New York, NY, USA.

Inhibition of oncogenes and reactivation of tumor suppressors are well-established goals in anticancer drug development. Unfortunately many oncogenes and tumor suppressors are not classically druggable, in that they lack a targetable enzymatic activity and associated binding pockets that small molecule drugs can be directed to. This is especially relevant for transcription factors, which have long been thought to be undruggable. Read More

View Article
January 2019