47,314 results match your criteria Biomedicine & Pharmacotherapy[Journal]


Emodin improves glucose metabolism by targeting microRNA-20b in insulin-resistant skeletal muscle.

Phytomedicine 2018 Nov 16;59:152758. Epub 2018 Nov 16.

Department of Pharmacology (State-Province Key Laboratories of Biomedicine-Pharmaceutics of China, Key Laboratory of Cardiovascular Research, Ministry of Education), Harbin Medical University, Harbin 150081, China; Department of Pharmacology and Therapeutics, Melbourne School of Biomedical Sciences, Faculty of Medicine, Dentistry and Health Sciences, the University of Melbourne, Melbourne, 3010, Australia. Electronic address:

Background: Emerging evidence has indicated the therapeutic potential of emodin with its multiple pharmacological effects.

Purpose: To evaluate role of emodin in regulating insulin resistance (IR) and to elucidate the underlying molecular mechanisms.

Study Design/methods: Fasting blood glucose (FBG) and lipid levels were measured before and after intragastric administration of emodin in type 2 diabetes mellitus (T2DM) rats. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S09447113183057
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http://dx.doi.org/10.1016/j.phymed.2018.11.018DOI Listing
November 2018
1 Read

Nanoemulsion strategy of pioglitazone for the treatment of skin inflammatory diseases.

Nanomedicine 2019 Apr 17. Epub 2019 Apr 17.

Institute of Nanoscience and Nanotechnology (IN2UB), University of Barcelona, 08028 Barcelona, Spain; Department of Pharmacy and Pharmaceutical Technology, Faculty of Pharmacy, University of Granada, Granada, Spain. Electronic address:

Pioglitazone (PGZ) is a peroxisome proliferator-activated receptor agonist. Its role in the inflammatory response modulation opens the door for additional therapeutic applications. The purpose of this study was to develop a pioglitazone nanoemulsion (PGZ-NE) to investigate its anti-inflammatory efficacy on the skin. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S15499634193007
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http://dx.doi.org/10.1016/j.nano.2019.03.017DOI Listing
April 2019
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Cytotoxic polyphenolic compounds from Lespedeza bicolor stem bark.

Fitoterapia 2019 Apr 17. Epub 2019 Apr 17.

G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, Prospect 100-let Vladivostoku 159, Vladivostok, 690022, Russia.

Four new pterocarpans (6aR,11aR)-6a,11a-dihydrolespedezol A (2), (6aR,11aR)-2-isoprenyl-6a,11a-dihydrolespedezol A (3), (6aR,11aR,3'R)-6a,11a-dihydrolespedezol A (4), (6aR,11aR,3'S)-6a,11a-dihydrolespedezol A (5) and one new stilbenoid with 1,2-diketone fragment named bicoloketone (6) along with one previously known pterocarpen lespedezol A (1) have been isolated from Lespedeza bicolor stem bark using multistage column chromatography on polyamide and silica gel. The structures of the isolated polyphenolic compounds were determined by spectroscopic methods. The absolute configurations of 4 and 5 were determined by comparison of their electronic circular dichroism (ECD) spectra obtained experimentally and the spectra calculated using time-dependent density functional theory (TDDFT). Read More

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http://dx.doi.org/10.1016/j.fitote.2019.04.003DOI Listing
April 2019
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Comparison of multiplex-serology and ELISA based methods in detecting HPV16 L1 antibody responses in paired saliva and serum samples of healthy men.

J Virol Methods 2019 Apr 17. Epub 2019 Apr 17.

Institute of Dentistry, University of Turku, Lemminkaisenkatu 2, 20520 Turku, Finland; Department of Pathology, Turku University Hospital, Kiinamyllynkatu 4-8, 20521 Turku, Finland.

Human papilloma viruses (HPV) are a common cause of transient infections on mucosal surfaces, also in the oral cavity. Some infections remain persistent and can, especially with high risk HPV genotypes, lead to malignancies in the oral-oropharyngeal area. Our understanding of the natural course of oral HPV infections is limited, and the local host responses are poorly known. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01660934183003
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http://dx.doi.org/10.1016/j.jviromet.2019.04.007DOI Listing
April 2019
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Author Correction: Markers of subclinical vascular damages associate with indices of adiposity and blood pressure in obese children.

Hypertens Res 2019 Apr 19. Epub 2019 Apr 19.

Department of Medicine, University of Verona, Verona, Italy.

Since the publication of this paper, it has been noted that the author Denise Marcon had been missed out of the author list. The correct author list is shown above. Read More

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http://dx.doi.org/10.1038/s41440-019-0262-2DOI Listing

MAPK pathway and B cells overactivation in multiple sclerosis revealed by phosphoproteomics and genomic analysis.

Proc Natl Acad Sci U S A 2019 Apr 19. Epub 2019 Apr 19.

Center of Neuroimmunology, Institut d'Investigacions Biomèdiques August Pi Sunyer, Barcelona 08036, Spain;

Dysregulation of signaling pathways in multiple sclerosis (MS) can be analyzed by phosphoproteomics in peripheral blood mononuclear cells (PBMCs). We performed in vitro kinetic assays on PBMCs in 195 MS patients and 60 matched controls and quantified the phosphorylation of 17 kinases using xMAP assays. Phosphoprotein levels were tested for association with genetic susceptibility by typing 112 single-nucleotide polymorphisms (SNPs) associated with MS susceptibility. Read More

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http://dx.doi.org/10.1073/pnas.1818347116DOI Listing
April 2019
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Polyphosphoinositides in the nucleus: Roadmap of their effectors and mechanisms of interaction.

Adv Biol Regul 2019 Apr 6. Epub 2019 Apr 6.

Department of Biological Sciences, University of Bergen, Bergen, 5008, Norway. Electronic address:

Biomolecular interactions between proteins and polyphosphoinositides (PPIn) are essential in the regulation of the vast majority of cellular processes. Consequently, alteration of these interactions is implicated in the development of many diseases. PPIn are phosphorylated derivatives of phosphatidylinositol and consist of seven species with different phosphate combinations. Read More

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http://dx.doi.org/10.1016/j.jbior.2019.04.001DOI Listing

TRPA1 Channel is Involved in SLIGRL-Evoked Thermal and Mechanical Hyperalgesia in Mice.

Med Sci (Basel) 2019 Apr 18;7(4). Epub 2019 Apr 18.

Laboratory of Pain and Analgesia, Beritashvili Center for Experimental Biomedicine, Tbilisi 0160, Georgia.

Persistent itch (pruritus) accompanying dermatologic and systemic diseases can significantly impair the quality of life. It is well known that itch is broadly categorized as histaminergic (sensitive to antihistamine medications) or non-histaminergic. Sensory neurons expressing Mas-related G-protein-coupled receptors (Mrgprs) mediate histamine-independent itch. Read More

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https://www.mdpi.com/2076-3271/7/4/62
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http://dx.doi.org/10.3390/medsci7040062DOI Listing
April 2019
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Quantitative Analysis of Nuclear Lamins Imaged by Super-Resolution Light Microscopy.

Cells 2019 Apr 18;8(4). Epub 2019 Apr 18.

Department of Cell and Molecular Biology, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA.

The nuclear lamina consists of a dense fibrous meshwork of nuclear lamins, Type V intermediate filaments, and is ~14 nm thick according to recent cryo-electron tomography studies. Recent advances in light microscopy have extended the resolution to a scale allowing for the fine structure of the lamina to be imaged in the context of the whole nucleus. We review quantitative approaches to analyze the imaging data of the nuclear lamina as acquired by structured illumination microscopy (SIM) and single molecule localization microscopy (SMLM), as well as the requisite cell preparation techniques. Read More

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https://www.mdpi.com/2073-4409/8/4/361
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http://dx.doi.org/10.3390/cells8040361DOI Listing
April 2019
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Biomass-Templated Fabrication of Metallic Materials for Photocatalytic and Bactericidal Applications.

Materials (Basel) 2019 Apr 18;12(8). Epub 2019 Apr 18.

Institute of Advanced Materials (IAM) & Key Laboratory of Flexible Electronics (KLOFE), Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM), Nanjing Tech University (NanjingTech), Nanjing 211816, China.

In this paper, we report a simple, feasible and low-cost method to fabricate self-standing metallic materials using cellulose-based biomass as sacrificial templates. This process involves the impregnation of metallic precursors to the cellulose fibers of biomass templates and the transformation of the precursors to corresponding metals or metal oxides (as well as the removal of the cellulose framework) at an elevated temperature. The structures of the metallic materials as fabricated take the form of architectures of biomass templates (e. Read More

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http://dx.doi.org/10.3390/ma12081271DOI Listing

Age-dependent variations in the expression of myosin isoforms and myogenic factors during the involution of the proximal sesamoidean ligament of sheep.

Res Vet Sci 2019 Apr 11;124:270-279. Epub 2019 Apr 11.

Department of Comparative Biomedicine and Food Science, Università di Padova, Italy. Electronic address:

In ungulates the stability of the fetlock joint is dependent on several muscles, which are exposed to high stress and strain. Among those muscles, the proximal sesamoidean ligament or PSL (also known as the suspensory ligament or Ruini's elasto-tendinous organ) is organized at birth in layers of muscle fibres alternated with abundant tendinous tissue that, during the postnatal development, becomes the predominant tissue. In this study we analysed the PSL of the sheep at the age of 1, 30 and 180 days and determined the expression of several genes which either (a) are markers of muscle fibre growth and maturation, or (b) play a role as signal molecules. Read More

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http://dx.doi.org/10.1016/j.rvsc.2019.04.006DOI Listing

Honokiol for cancer therapeutics: A traditional medicine that can modulate multiple oncogenic targets.

Pharmacol Res 2019 Apr 16. Epub 2019 Apr 16.

Cancer Biology Laboratory and DBT-AIST International Laboratory for Advanced Biomedicine (DAILAB) Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Assam, 781039, India. Electronic address:

In spite of billions of dollars expended on cancer research every year, the incidence rate and the mortality rate due to this widespread disease has increased drastically over the last few decades. Recent reports from the World Health Organization advocate that overall global cancer burden and deaths due to cancer are expected to double by the next decade. Synthetic drugs developed as chemotherapeutics have repeatedly shown adverse side effects and development of chemoresistance. Read More

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http://dx.doi.org/10.1016/j.phrs.2019.04.004DOI Listing
April 2019
2 Reads

Corrigendum to Stage-specific metabolic features of differentiating neurons: Implications for toxicant sensitivity Toxicol Appl Pharmacol, 354 (2018) 64-80.

Toxicol Appl Pharmacol 2019 Apr 16. Epub 2019 Apr 16.

In Vitro Toxicology and Biomedicine, Dept Inaugurated by the Doerenkamp-Zbinden Foundation, University of Konstanz, 78457 Konstanz, Germany; CAAT-Europe, University of Konstanz, Konstanz 78457, Germany. Electronic address:

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http://dx.doi.org/10.1016/j.taap.2019.04.010DOI Listing

miR‑145‑5p is associated with pathological complete response to neoadjuvant chemotherapy and impairs cell proliferation by targeting TGFβR2 in breast cancer.

Oncol Rep 2019 Apr 5. Epub 2019 Apr 5.

Genomics Sciences Program, Autonomous University of Mexico City, Mexico City 03100, Mexico.

Cancer patients who better benefit from neoadjuvant chemotherapy (NeoCh) are those who achieve a successful pathological complete response (pCR) represented by the absence of residual disease. Unfortunately, no highly sensitive and specific tumor biomarkers for predicting the clinical response to NeoCh have yet been defined. The aim of the present study was to ascertain whether miR‑145‑5p could discriminate between pCR and no‑pCR in triple‑negative breast cancer patients that received a cisplatin/doxorubicin‑based neoadjuvant treatment. Read More

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http://dx.doi.org/10.3892/or.2019.7102DOI Listing

Metal Binding Antimicrobial Peptides in Nanoparticle Bio-functionalization: New Heights in Drug Delivery and Therapy.

Probiotics Antimicrob Proteins 2019 Apr 18. Epub 2019 Apr 18.

Biochemistry Department, Faculty of Science, Ege University, 35100, Bornova, Izmir, Turkey.

Peptides are considered very important due to the diversity expressed through their amino acid sequence, structure variation, large spectrum, and their essential role in biological systems. Antimicrobial peptides (AMPs) emerged as a potent tool in therapy owing to their antimicrobial properties but also their ability to trespass the membranes, specificity, and low toxicity. They comprise a variety of peptides from which specific amino acid-rich peptides are of interest to the current review due to their features in metal interaction and cell penetration. Read More

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http://link.springer.com/10.1007/s12602-019-09546-5
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http://dx.doi.org/10.1007/s12602-019-09546-5DOI Listing
April 2019
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Early Hearing Loss upon Disruption of Slc4a10 in C57BL/6 Mice.

J Assoc Res Otolaryngol 2019 Apr 18. Epub 2019 Apr 18.

Institute of Human Genetics, Jena University Hospital, Friedrich Schiller Universität, Am Klinikum 1, 07747, Jena, Germany.

The unique composition of the endolymph with a high extracellular K concentration is essential for sensory transduction in the inner ear. It is secreted by a specialized epithelium, the stria vascularis, that is connected to the fibrocyte meshwork of the spiral ligament in the lateral wall of the cochlea via gap junctions. In this study, we show that in mice the expression of the bicarbonate transporter Slc4a10/Ncbe/Nbcn2 in spiral ligament fibrocytes starts shortly before hearing onset. Read More

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http://link.springer.com/10.1007/s10162-019-00719-1
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http://dx.doi.org/10.1007/s10162-019-00719-1DOI Listing
April 2019
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Critical role of spectrin in hearing development and deafness.

Sci Adv 2019 Apr 17;5(4):eaav7803. Epub 2019 Apr 17.

iHuman Institute, ShanghaiTech University, Shanghai, China.

Inner ear hair cells (HCs) detect sound through the deflection of mechanosensory stereocilia. Stereocilia are inserted into the cuticular plate of HCs by parallel actin rootlets, where they convert sound-induced mechanical vibrations into electrical signals. The molecules that support these rootlets and enable them to withstand constant mechanical stresses underpin our ability to hear. Read More

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http://advances.sciencemag.org/lookup/doi/10.1126/sciadv.aav
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http://dx.doi.org/10.1126/sciadv.aav7803DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6469942PMC
April 2019
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CACNA2D3 Enhances the Chemosensitivity of Esophageal Squamous Cell Carcinoma to Cisplatin via Inducing Ca-Mediated Apoptosis and Suppressing PI3K/Akt Pathways.

Front Oncol 2019 2;9:185. Epub 2019 Apr 2.

Department of Cell Biology, Institute of Biomedicine, Jinan University, Guangzhou, China.

Resistance to platinum-based combination chemotherapy is the main cause of poor prognosis in patients with advanced esophageal squamous cell carcinoma (ESCC). Previously, we showed that CACNA2D3 (voltage-dependent subunit alpha 2 delta 3 of a calcium channel complex) was significantly downregulated and functioned as a tumor suppressor in ESCC, but its role in the chemosensitivity of ESCC to cisplatin remained unknown. Here, we found that the expression of CACNA2D3 was significantly associated with poor platinum response in ESCC patients from the Gene Expression Omnibus database. Read More

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http://dx.doi.org/10.3389/fonc.2019.00185DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6454090PMC

Feto-Maternal Microchimerism: The Pre-eclampsia Conundrum.

Front Immunol 2019 29;10:659. Epub 2019 Mar 29.

Department of Biomedicine, University Hospital Basel, Basel, Switzerland.

Feto-maternal microchimerism (FMM) involves bidirectional cross-placental trafficking during pregnancy, leading to a micro-chimeric state that can persist for decades. In this manner a pregnant woman will harbor cells from her mother, as well as, cells from her child. Historically, eclampsia, a severe disorder of pregnancy provided the basis for FMM following the detection of trophoblast cells in the lungs of deceased women. Read More

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https://www.frontiersin.org/article/10.3389/fimmu.2019.00659
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http://dx.doi.org/10.3389/fimmu.2019.00659DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6455070PMC
March 2019
1 Read

Treatment algorithm for spontaneous spinal infections: A review of the literature.

J Craniovertebr Junction Spine 2019 Jan-Mar;10(1):3-9

Department of Human Neurosciences, Division of Neurosurgery, Sapienza University of Rome, Rome, Italy.

Background: Primary spinal infections are rare pathologies with an estimated incidence of 5% of all osteomyelitis. The diagnosis can be challenging and this might result in a late identification. The etiological diagnosis is the primary concern to determine the most appropriate treatment. Read More

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http://dx.doi.org/10.4103/jcvjs.JCVJS_115_18DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6469318PMC

Anti-Stress and Glial Differentiation Effects of a Novel Combination of Cucurbitacin B and Withanone (CucWi-N): Experimental Evidence.

Ann Neurosci 2018 Dec 5;25(4):201-209. Epub 2018 Jul 5.

DBT-AIST International laboratory for Advanced Biomedicine (DAILAB), National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Japan.

Background: Natural extracts and compounds used in traditional home medicine are known for their safety and a variety of health promoting and therapeutic potentials. In contrast to the single molecule mediated targets, the combinational therapies are preferred for their multi-functional and limited toxic regimens and may be useful for disease therapeutics as well as to increase the quality of life during a variety of environmental stresses.

Purpose: We aimed to combine the active ingredients of Chinese () and Indian () ginsengs to develop a natural, efficient, and welfare combinatorial mixture with high anti-stress and glial differentiation potentials. Read More

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https://www.karger.com/Article/FullText/490693
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http://dx.doi.org/10.1159/000490693DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6470339PMC
December 2018
1 Read

Isolation and characterisation of pVa-21, a giant bacteriophage with anti-biofilm potential against Vibrio alginolyticus.

Sci Rep 2019 Apr 18;9(1):6284. Epub 2019 Apr 18.

Laboratory of Aquatic Biomedicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul, Republic of Korea.

There is an increasing emergence of antibiotic-resistant Vibrio alginolyticus, a zoonotic pathogen that causes mass mortality in aquatic animals and infects humans; therefore, there is a demand for alternatives to antibiotics for the treatment and prevention of infections caused by this pathogen. One possibility is through the exploitation of bacteriophages. In the present study, the novel bacteriophage pVa-21 was classified as Myoviridae and characterised as a candidate biocontrol agent against V. Read More

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http://www.nature.com/articles/s41598-019-42681-1
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http://dx.doi.org/10.1038/s41598-019-42681-1DOI Listing
April 2019
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Common patterns of gene regulation associated with Cesarean section and the development of islet autoimmunity - indications of immune cell activation.

Sci Rep 2019 Apr 18;9(1):6250. Epub 2019 Apr 18.

Institute of Computational Biology, Helmholtz Zentrum München, Neuherberg, Germany.

Birth by Cesarean section increases the risk of developing type 1 diabetes later in life. We aimed to elucidate common regulatory processes observed after Cesarean section and the development of islet autoimmunity, which precedes type 1 diabetes, by investigating the transcriptome of blood cells in the developing immune system. To investigate Cesarean section effects, we analyzed longitudinal gene expression profiles from peripheral blood mononuclear cells taken at several time points from children with increased familial and genetic risk for type 1 diabetes. Read More

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http://dx.doi.org/10.1038/s41598-019-42750-5DOI Listing

Felodipine induces autophagy in mouse brains with pharmacokinetics amenable to repurposing.

Nat Commun 2019 Apr 18;10(1):1817. Epub 2019 Apr 18.

Department of Medical Genetics, Cambridge Institute for Medical Research, University of Cambridge, The Keith Peters Building, Cambridge Biomedical Campus, Hills Road, Cambridge, CB2 0XY, UK.

Neurodegenerative diseases like Alzheimer's disease, Parkinson's disease and Huntington's disease manifest with the neuronal accumulation of toxic proteins. Since autophagy upregulation enhances the clearance of such proteins and ameliorates their toxicities in animal models, we and others have sought to re-position/re-profile existing compounds used in humans to identify those that may induce autophagy in the brain. A key challenge with this approach is to assess if any hits identified can induce neuronal autophagy at concentrations that would be seen in humans taking the drug for its conventional indication. Read More

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http://www.nature.com/articles/s41467-019-09494-2
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http://dx.doi.org/10.1038/s41467-019-09494-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6472390PMC
April 2019
3 Reads

L amino acid transporter structure and molecular bases for the asymmetry of substrate interaction.

Nat Commun 2019 Apr 18;10(1):1807. Epub 2019 Apr 18.

Institute for Research in Biomedicine (IRB Barcelona), Barcelona Institute of Science and Technology, 08028, Barcelona, Spain.

L-amino acid transporters (LATs) play key roles in human physiology and are implicated in several human pathologies. LATs are asymmetric amino acid exchangers where the low apparent affinity cytoplasmic side controls the exchange of substrates with high apparent affinity on the extracellular side. Here, we report the crystal structures of an LAT, the bacterial alanine-serine-cysteine exchanger (BasC), in a non-occluded inward-facing conformation in both apo and substrate-bound states. Read More

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http://dx.doi.org/10.1038/s41467-019-09837-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6472337PMC

Dishevelled-3 conformation dynamics analyzed by FRET-based biosensors reveals a key role of casein kinase 1.

Nat Commun 2019 Apr 18;10(1):1804. Epub 2019 Apr 18.

Department of Experimental Biology, Faculty of Science, Masaryk University, Brno, 62500, Czech Republic.

Dishevelled (DVL) is the key component of the Wnt signaling pathway. Currently, DVL conformational dynamics under native conditions is unknown. To overcome this limitation, we develop the Fluorescein Arsenical Hairpin Binder- (FlAsH-) based FRET in vivo approach to study DVL conformation in living cells. Read More

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http://www.nature.com/articles/s41467-019-09651-7
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http://dx.doi.org/10.1038/s41467-019-09651-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6472409PMC
April 2019
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Amygdala ensembles encode behavioral states.

Science 2019 Apr 18;364(6437). Epub 2019 Apr 18.

Friedrich Miescher Institute for Biomedical Research, Maulbeerstrasse 66, Basel, Switzerland.

Internal states, including affective or homeostatic states, are important behavioral motivators. The amygdala regulates motivated behaviors, yet how distinct states are represented in amygdala circuits is unknown. By longitudinally imaging neural calcium dynamics in freely moving mice across different environments, we identified opponent changes in activity levels of two major, nonoverlapping populations of basal amygdala principal neurons. Read More

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http://dx.doi.org/10.1126/science.aav8736DOI Listing

Enhanced dendritic inhibition and impaired NMDAR activation in a mouse model of Down syndrome.

J Neurosci 2019 Apr 18. Epub 2019 Apr 18.

Department of Biomedicine, University of Basel, Pestalozzistr. 20, CH-4056 Basel, Switzerland

Down syndrome (DS) or Trisomy 21 is a developmental disorder leading to cognitive deficits including disruption of hippocampus-dependent learning and memory. Enhanced inhibition has been suggested to underlie these deficits in DS based on studies using the Ts65Dn mouse model. Here we show that in this mouse model, GABAergic synaptic inhibition onto dendrites of hippocampal pyramidal cells is increased. Read More

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http://www.jneurosci.org/lookup/doi/10.1523/JNEUROSCI.2723-1
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http://dx.doi.org/10.1523/JNEUROSCI.2723-18.2019DOI Listing
April 2019
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Complete Genome Sequence of a Bioactive Pseudomonas sp. Strain, DTU12.3, Isolated from Soil in Denmark.

Microbiol Resour Announc 2019 Apr 18;8(16). Epub 2019 Apr 18.

Department of Biotechnology and Biomedicine, Technical University of Denmark, Kongens Lyngby, Denmark

Here, we report the complete annotated genome sequence of a sp. strain, DTU12.3. Read More

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http://dx.doi.org/10.1128/MRA.00121-19DOI Listing

Mouse ANKRD31 Regulates Spatiotemporal Patterning of Meiotic Recombination Initiation and Ensures Recombination between X and Y Sex Chromosomes.

Mol Cell 2019 Apr 12. Epub 2019 Apr 12.

Institute of Physiological Chemistry, Faculty of Medicine, Technische Universität Dresden, Fetscherstraße 74, 01307 Dresden, Germany. Electronic address:

Orderly segregation of chromosomes during meiosis requires that crossovers form between homologous chromosomes by recombination. Programmed DNA double-strand breaks (DSBs) initiate meiotic recombination. We identify ANKRD31 as a key component of complexes of DSB-promoting proteins that assemble on meiotic chromosome axes. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S10972765193022
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http://dx.doi.org/10.1016/j.molcel.2019.03.022DOI Listing
April 2019
4 Reads

New insights into the binding mechanism between osthole and β-lactoglobulin: Spectroscopic, chemometrics and docking studies.

Food Res Int 2019 Jun 22;120:226-234. Epub 2019 Feb 22.

State Key Laboratory of Food Science and Technology, Nanchang University, 235 Nanjing East Road, Nanchang 330047, China. Electronic address:

Osthole, a type of coumarin derivative, owns many biological functions, but the poor water solubility and low bioavailability limit its usage in food and pharmaceutical fields. β-Lactoglobulin (BLG) is a major protein in bovine milk whey which is usually used as a carrier of poorly soluble bioactive components. Researching the interaction between osthole and BLG can help us in understanding their binding mechanism and design an osthole delivery system using BLG as carrier protein to further promote the application of osthole in functional food and drug. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S09639969193012
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http://dx.doi.org/10.1016/j.foodres.2019.02.042DOI Listing
June 2019
1 Read

T2, T2 and spin coupling ratio as biomarkers for the study of lipomatous tumors.

Phys Med 2019 Apr 28;60:76-82. Epub 2019 Mar 28.

Department of Radiology, University of Crete, Heraklion, Crete, Greece.

Background: Subcutaneous fat may have variable signal intensity on T2w images depending on the choice of imaging parameters. However, fatty components within tumors have a different degree of signal dependence on the acquisition scheme. This study examined the use of T2, T2 relaxometry and spin coupling related signal changes (Spin Coupling ratio, SCr) on two different imaging protocols as clinically relevant descriptors of benign and malignant lipomatous tumors. Read More

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http://dx.doi.org/10.1016/j.ejmp.2019.03.023DOI Listing

Impact of polyols on Oral microbiome of Estonian schoolchildren.

BMC Oral Health 2019 Apr 18;19(1):60. Epub 2019 Apr 18.

Department of Microbiology, Institute of Biomedicine and Translational Medicine, University of Tartu, Tartu, Estonia.

Background: Oral microbiome has significant impact on both oral and general health. Polyols have been promoted as sugar substitutes in prevention of oral diseases. We aimed to reveal the effect of candies containing erythritol, xylitol or control (sorbitol) on salivary microbiome. Read More

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https://bmcoralhealth.biomedcentral.com/articles/10.1186/s12
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http://dx.doi.org/10.1186/s12903-019-0747-zDOI Listing
April 2019
1 Read

Metabolic and signalling network maps integration: application to cross-talk studies and omics data analysis in cancer.

BMC Bioinformatics 2019 Apr 18;20(Suppl 4):140. Epub 2019 Apr 18.

Institut Curie, 26 rue d'Ulm, F-75005, Paris, France.

Background: The interplay between metabolic processes and signalling pathways remains poorly understood. Global, detailed and comprehensive reconstructions of human metabolism and signalling pathways exist in the form of molecular maps, but they have never been integrated together. We aim at filling in this gap by integrating of both signalling and metabolic pathways allowing a visual exploration of multi-level omics data and study of cross-regulatory circuits between these processes in health and in disease. Read More

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http://dx.doi.org/10.1186/s12859-019-2682-zDOI Listing
April 2019
2 Reads

Dose-Response Meta-analysis: Application and Practice using R software.

Epidemiol Health 2019 Mar 28:e2019006. Epub 2019 Mar 28.

Department of Internal Medicine, Jeju National University Hospital, Jeju National University School of Medicine, Jeju, Korea.

The objective of this study was to describe general approaches of dose-response meta-analysis that are available for quantitative synthesis of data using R software. We conducted a dose-response meta-analysis using two types of data that included difference in means in continuous data and odds ratio in binary data. The package commands of R software were doseresmeta for overall effect sizes that were separated linear model, quadratic model, and restricted cubic split model in order to well understanding. Read More

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http://e-epih.org/journal/view.php?doi=10.4178/epih.e2019006
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http://dx.doi.org/10.4178/epih.e2019006DOI Listing
March 2019
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Diagnostic Test Accuracy: Application and Practice using R software.

Epidemiol Health 2019 Mar 28:e2019007. Epub 2019 Mar 28.

Department of Internal Medicine, Jeju National University Hospital, Jeju National University School of Medicine, Jeju, Korea.

The objective of this study was to describe general approaches of diagnostic test accuracy that are available for quantitative synthesis of data using R software. We conducted a diagnostic test accuracy that summarized statistics for univariate analysis and bivariate analysis. The package commands of R software were metaprop and metabin for sensitivity, specificity, and diagnostic odds ratio, forest for forest plot, reitsma of mada for summarized ROC curve, and metareg for meta-regression analysis. Read More

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http://e-epih.org/journal/view.php?doi=10.4178/epih.e2019007
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http://dx.doi.org/10.4178/epih.e2019007DOI Listing
March 2019
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Intervention Meta-analysis: Application and Practice using R software.

Epidemiol Health 2019 Mar 28:e2019008. Epub 2019 Mar 28.

Department of Nuclear Medicine, College of Medicine, Pusan National University, Yangsan, Korea.

The objective of this study was to describe general approaches of intervention meta-analysis that are available for quantitative synthesis of data using R software. We conducted an intervention meta-analysis using two types of data that included difference in means in continuous data and odds ratio in binary data. The package commands of R software were metacont, metabin, and metagen for overall effect size, forest for forest plot, metareg for meta-regression analysis, and funnel and metabias for publication bias. Read More

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http://dx.doi.org/10.4178/epih.e2019008DOI Listing

Network Meta-analysis: Application and Practice using R software.

Epidemiol Health 2019 Apr 8:e2019013. Epub 2019 Apr 8.

Institute of Medical Biometry and Statistics, Faculty of Medicine and Medical Center - University of Freiburg, Freiburg, Germany.

The objective of this study was to describe general approaches of network meta-analysis that are available for quantitative synthesis of data using R software. We conducted a network meta-analysis using two types of concepts: Bayesian and frequentist approach. The packages of R software were gemtc for Bayesian approach and netmeta for frequentist approach. Read More

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http://e-epih.org/journal/view.php?doi=10.4178/epih.e2019013
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http://dx.doi.org/10.4178/epih.e2019013DOI Listing
April 2019
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Advances in the Understanding of Skin Cancer: Ultraviolet Radiation, Mutations, and Antisense Oligonucleotides as Anticancer Drugs.

Molecules 2019 Apr 17;24(8). Epub 2019 Apr 17.

Medical Academy named after S.I. Georgievsky, V.I. Vernadsky Crimean Federal University, Lenin Boulevard 5/7, 295051 Simferopol, Crimea.

Skin cancer has always been and remains the leader among all tumors in terms of occurrence. One of the main factors responsible for skin cancer, natural and artificial UV radiation, causes the mutations that transform healthy cells into cancer cells. These mutations inactivate apoptosis, an event required to avoid the malignant transformation of healthy cells. Read More

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https://www.mdpi.com/1420-3049/24/8/1516
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http://dx.doi.org/10.3390/molecules24081516DOI Listing
April 2019
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Effects of Neck Taping in the Treatment of Hemispatial Neglect in Chronic Stroke Patients: A Pilot, Single Blind, Randomized Controlled Trial.

Medicina (Kaunas) 2019 Apr 17;55(4). Epub 2019 Apr 17.

Neuromotor and Cognitive Rehabilitation Research Center, Department of Neurosciences, Biomedicine and Movement Sciences, University of Verona, 37134 Verona, Italy.

Hemispatial neglect is a common consequence of stroke, with an estimated incidence of 23%. Interventions for treating hemispatial neglect may be categorized as either top-down or bottom-up processing. The aim of top-down approaches is to train the person to voluntarily compensate for their neglect. Read More

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https://www.mdpi.com/1010-660X/55/4/108
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http://dx.doi.org/10.3390/medicina55040108DOI Listing
April 2019
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The PTEN⁻PI3K Axis in Cancer.

Biomolecules 2019 Apr 17;9(4). Epub 2019 Apr 17.

Cancer Research Institute, Beth Israel Deaconess Cancer Center, Department of Medicine and Pathology, Beth Israel Deaconess Medical Center (BIDMC), Harvard Medical School, Boston, MA 02215, USA.

The PI3K-AKT-mTOR signal transduction pathway regulates a variety of biological processes including cell growth, cell cycle progression and proliferation, cellular metabolism, and cytoskeleton reorganization. Fine-tuning of the phosphatidylinositol 3-kinase (PI3K) pathway signaling output is essential for the maintenance of tissue homeostasis and uncontrolled activation of this cascade leads to a number of human pathologies including cancer. Inactivation of the tumor suppressor phosphatase and tensin homologue deleted on Chromosome 10 (PTEN) and/or activating mutations in the proto-typical lipid kinase PI3K have emerged as some of the most frequent events associated with human cancer and as a result the PI3K pathway has become a highly sought-after target for cancer therapies. Read More

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https://www.mdpi.com/2218-273X/9/4/153
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http://dx.doi.org/10.3390/biom9040153DOI Listing
April 2019
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Vasculogenic Chronic Ulcer: Tissue Regeneration with an Innovative Dermal Substitute.

J Clin Med 2019 Apr 17;8(4). Epub 2019 Apr 17.

Department of Surgical Science, Plastic and Reconstructive Surgery Unit, University of Rome Tor Vergata, 00133 Rome, Italy.

The healing of venous and arterial ulcers is slow, and in some cases, they may not heal at all. This study aims to demonstrate the clinical advantage of Nevelia, an innovative collagen dermal template substitute (DS) in venous and arterial chronic ulcers treatment. 35 patients affected by chronic vascular ulcers with a mean area of 35. Read More

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https://www.mdpi.com/2077-0383/8/4/525
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http://dx.doi.org/10.3390/jcm8040525DOI Listing
April 2019
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Screening for mutations in selected miRNA genes in hypogonadotropic hypogonadism patients.

Endocr Connect 2019 May;8(5):506-509

Institute of Biomedicine/Physiology, Biomedicum Helsinki and Stem Cells and Metabolism Research Program, University of Helsinki, Helsinki, Finland.

In approximately half of congenital hypogonadotropic hypogonadism (cHH) patients, the genetic cause remains unidentified. Since the lack of certain miRNAs in animal models has led to cHH, we sequenced human miRNAs predicted to regulate cHH-related genes (MIR7-3, MIR141, MIR429 and MIR200A-C) in 24 cHH patients with Sanger sequencing. A heterozygous variant in MIR200A (rs202051309; general population frequency of 0. Read More

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https://ec.bioscientifica.com/view/journals/ec/8/5/EC-19-008
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http://dx.doi.org/10.1530/EC-19-0080DOI Listing
May 2019
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On the critical evaluation and confirmation of germline sequence variants identified using massively parallel sequencing.

J Biotechnol 2019 Apr 15. Epub 2019 Apr 15.

Institute for Clinical and Translational Research, Biomedical Research Center, Slovak Academy of Sciences, Bratislava, Slovakia; Geneton Ltd., Bratislava, Slovakia. Electronic address:

Although massively parallel sequencing (MPS) is becoming common practice in both research and routine clinical care, confirmation requirements of identified DNA variants using alternative methods are still topics of debate. When evaluating variants directly from MPS data, different read depth statistics, together with specialized genotype quality scores are, therefore, of high relevance. Here we report results of our validation study performed in two different ways: 1) confirmation of MPS identified variants using Sanger sequencing; and 2) simultaneous Sanger and MPS analysis of exons of selected genes. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01681656193012
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http://dx.doi.org/10.1016/j.jbiotec.2019.04.013DOI Listing
April 2019
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Native aortic versus mitral valve infective endocarditis: a nationwide registry study.

Open Heart 2019 27;6(1):e000926. Epub 2019 Feb 27.

Division of Cardiothoracic Surgery, Department for Clinical Sciences Lund, Skane University Hospital and Lund University, Lund, Sweden.

Background: Native aortic and mitral valve infective endocarditis (AVE and MVE, respectively) are usually grouped together as left-sided native valve infective endocarditis (LNVE), while the differences between AVE and MVE have not yet been properly investigated. We aimed to compare AVE and MVE in regard to patient characteristics, microbiology and determinants of survival.

Methods: We conducted a retrospective study using the Swedish national registry on infective endocarditis, which contains nationwide patient data. Read More

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http://openheart.bmj.com/lookup/doi/10.1136/openhrt-2018-000
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http://dx.doi.org/10.1136/openhrt-2018-000926DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6443117PMC
February 2019
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PM induced neurodegenerative-like changes in mice and the antagonistic effects of vitamin E.

Toxicol Res (Camb) 2019 Mar 8;8(2):172-179. Epub 2019 Jan 8.

Department of Food science and Engineering , Moutai Institute , Renhuai , 564507 , China.

Neurodegenerative diseases (ND) affect a large and ever-growing population globally, resulting in heavy burdens for patients and their families. Though some specific genes related to ND have been identified, the genetic factors fail to fully account for the pathogenesis of ND. Therefore, the roles of the environment and life styles in the occurrence of ND are being actively probed. Read More

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http://xlink.rsc.org/?DOI=C8TX00333E
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http://dx.doi.org/10.1039/c8tx00333eDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6425994PMC
March 2019
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Functional black phosphorus nanosheets for mitochondria-targeting photothermal/photodynamic synergistic cancer therapy.

Chem Sci 2019 Apr 21;10(13):3779-3785. Epub 2019 Feb 21.

Key Laboratory of Flexible Electronics (KLOFE) , Institute of Advanced Materials (IAM) , School of Physical and Mathematical Science , Nanjing Tech University (NanjingTech) , 30 South Puzhu Road , Nanjing 211800 , China . Email: ; Email: ; Email:

Organelle-targeting nanosystems are envisioned as promising tools for phototherapy, which can generate heat or reactive oxygen species (ROS) induced cytotoxicity in the targeted location but leave the surrounding biological tissues undamaged. In this work, an imaging-guided mitochondria-targeting photothermal/photodynamic nanosystem has been developed on the basis of functionalized black phosphorus (BP) nanosheets (NSs). In the nanosystem, BP NSs are coated with polydopamine (PDA) and then covalently linked with both chlorin e6 (Ce6) and triphenyl phosphonium (TPP) through carbodiimide reaction between the amino group and the carboxyl group, forming BP@PDA-Ce6&TPP NSs. Read More

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http://dx.doi.org/10.1039/c8sc04844dDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6446962PMC