45,896 results match your criteria Biomedicine & Pharmacotherapy[Journal]


Alpha lipoic acid reverses the negative effect of LPS on mouse spermatozoa and developmental competence of resultant embryos in vitro.

Andrology 2019 Feb 20. Epub 2019 Feb 20.

Department of Reproductive Biotechnology at Reproductive Biomedicine Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran.

Background: Reproductive toxicity of lipopolysaccharide (LPS) on spermatozoa is well established.

Objective: The aim of the present study was to show the potential benefits of alpha lipoic acid (ALA) as a strong antioxidant in alleviating the reproductive toxicity of LPS.

Materials And Methods: Sperm cells and cumulus-oocyte complexes (COCs) were collected from healthy NMRI mice (body weights ranged from 25 to 35 g, 100 females and 200 males). Read More

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http://dx.doi.org/10.1111/andr.12596DOI Listing
February 2019

Angiotensin II and salt-induced decompensation in Balb/CJ mice is aggravated by fluid retention related to low oxidative stress.

Am J Physiol Renal Physiol 2019 Feb 20. Epub 2019 Feb 20.

Department of medical cellbiology, Uppsala Universitet, Sweden.

Aim: Balb/CJ mice are more sensitive to treatment with Angiotensin II (AngII) and high salt (Salt) diet compared to C57BL/6J. Together with higher mortality, they develop edema, signs of heart failure and acute kidney injury. The aim of the present study was to identify differences in renal gene regulation that may affect kidney function and fluid balance, which could contribute to decompensation in Balb/CJ after AngII+Salt treatment. Read More

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http://dx.doi.org/10.1152/ajprenal.00483.2018DOI Listing
February 2019

Lymphotoxin-α promotes tumor angiogenesis in HNSCC by modulating glycolysis in a PFKFB3 dependent manner.

Int J Cancer 2019 Feb 20. Epub 2019 Feb 20.

Department of Oral and Maxillofacial Surgery, School & Hospital of Stomatology, Wuhan University, Wuhan, 430079, China.

Tumor angiogenesis is critical for tumor progression as the new blood vessels supply nutrients and facilitate metastasis. Previous studies indicate tumor associated lymphocytes, including B cells and T cells, contribute to tumor angiogenesis and tumor progression. The present study aims to identify the function of Lymphotoxin-α (LT-α), which is secreted by the activated lymphocytes, in the tumor angiogenesis of head and neck squamous cell carcinoma (HNSCC). Read More

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http://dx.doi.org/10.1002/ijc.32221DOI Listing
February 2019

Identification of atheroprone shear stress responsive regulatory elements in endothelial cells.

Cardiovasc Res 2019 Feb 20. Epub 2019 Feb 20.

Institute of Anatomy and Vascular Biology, Westfälische Wilhelms-Universität Münster, Vesaliusweg 2-4, 48149 Münster, Germany.

Aim: Oscillatory shear stress (OSS) is an atheroprone hemodynamic force that occurs in areas of vessel irregularities and is implicated in the pathogenesis of atherosclerosis. Changes in signaling and transcriptional program in response to OSS have been vigorously studied, however, the underlying changes in the chromatin landscape controlling transcription remain to be elucidated. Here, we investigated the changes in the regulatory element (RE) landscape of endothelial cells under atheroprone OSS conditions in an in vitro model. Read More

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http://dx.doi.org/10.1093/cvr/cvz027DOI Listing
February 2019
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Hybridization chain reaction-based nanoprobe for cancer cell recognition and amplified photodynamic therapy.

Chem Commun (Camb) 2019 Feb 20. Epub 2019 Feb 20.

Molecular Science and Biomedicine Laboratory, State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, P. R. China.

Precision diagnosis and effective treatment are the cores of early cancer therapy. Here, for the first time, we report a hybridization chain reaction-based nanoprobe for selective and sensitive cancer cell recognition and amplified photodynamic therapy. Read More

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http://dx.doi.org/10.1039/c8cc10074hDOI Listing
February 2019

NR4A Nuclear Receptors Target Poly-ADP-Ribosylated DNA-PKcs Protein to Promote DNA Repair.

Cell Rep 2019 Feb;26(8):2028-2036.e6

MRC Toxicology Unit, Leicester LE1 7HB, UK. Electronic address:

Although poly-ADP-ribosylation (PARylation) of DNA repair factors had been well documented, its role in the repair of DNA double-strand breaks (DSBs) is poorly understood. NR4A nuclear orphan receptors were previously linked to DSB repair; however, their function in the process remains elusive. Classically, NR4As function as transcription factors using a specialized tandem zinc-finger DNA-binding domain (DBD) for target gene induction. Read More

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http://dx.doi.org/10.1016/j.celrep.2019.01.083DOI Listing
February 2019
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Studying trans-acting enzymes that target carrier protein-bound amino acids during nonribosomal peptide synthesis.

Methods Enzymol 2019 11;617:113-154. Epub 2019 Feb 11.

Department of Biochemistry and Molecular Biology and ARC Centre of Excellence in Advanced Molecular Imaging, The Monash Biomedicine Discovery Institute, Monash University, Clayton, VIC, Australia; EMBL Australia, Monash University, Clayton, VIC, Australia. Electronic address:

Nonribosomal peptide biosynthesis is a complex enzymatic assembly responsible for producing a great diversity of bioactive peptide natural products. Due to the recurring arrangement of catalytic domains within these machineries, great interest has been shown in reengineering these pathways to produce novel, designer peptide products. However, in order to realize such ambitions, it is first necessary to develop a comprehensive understanding of the selectivity, mechanisms, and structure of these complex enzymes, which in turn requires significant in vitro experiments. Read More

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http://dx.doi.org/10.1016/bs.mie.2018.12.008DOI Listing
February 2019

Combination of Coral UTI Screen system, gram-stain and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for diagnosis of urinary tract infections directly from urine samples.

J Chemother 2019 Feb 20:1-7. Epub 2019 Feb 20.

a UGC Microbiology , Reina Sofía University Hospital , Córdoba , Spain.

This study proposes an algorithm for microbiological diagnosis of urinary tract infections based on screening by luminometry and Gram-stain, followed by identification by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS). Positive urine samples detected with the luminometry screening Coral UTI Screen system underwent Gram staining and identification of the causative organism was performed by MALDI-TOF Microflex LT mass spectrometer (Bruker Daltonics, Germany). Subsequently, the results were compared with those of conventional culture identification using WIDER MIC/id system (Francisco Soria Melguizo SA, Spain). Read More

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http://dx.doi.org/10.1080/1120009X.2019.1573284DOI Listing
February 2019

Comparative analysis of the influence of a high-fat/high-carbohydrate diet on the level of anxiety and neuromotor and cognitive functions in Wistar and DAT-KO rats.

Physiol Rep 2019 Feb;7(4):e13987

Federal Research Centre of Nutrition and Biotechnology, Moscow, Russia.

We compared anxiety, neuromotor, and cognitive functions in mutant rats with different allelic variants of dopamine transporter DAT knockout receiving balanced or excess in fat and fructose diet. The experiments were performed in DAT-/- homozygotes, DAT+/- heterozygotes, and DAT+/+ wild type rats. The genotype of DAT-KO rats was confirmed by restriction analysis of DAT gene compared to behavioral responses in the open field test (OF). Read More

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http://dx.doi.org/10.14814/phy2.13987DOI Listing
February 2019

Hepatitis E virus strains infecting Swedish domestic pigs are unique for each pig farm and remain in the farm for at least two years.

Transbound Emerg Dis 2019 Feb 19. Epub 2019 Feb 19.

Dep. of Infectious Diseases, Inst. of Biomedicine, Sahlgrenska Academy, Gothenburg University, Gothenburg, Sweden.

Hepatitis E virus (HEV) genotype 3 (HEV3) is distributed globally and infects both humans and animals, mainly domestic pigs and wild boars, which are the major reservoirs. In this study, the prevalence of HEV among Swedish pigs was investigated by HEV RNA analysis in 363 fecal samples from 3-month old piglets sampled twice (2013 and 2014) in 30 Swedish pig farms. Four different types of farms were investigated; organic, conventional closed (keeping the sow), satellites in a sow pool (conventional farms sharing sows), and conventional non-closed farms (purchasing gilts). Read More

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http://doi.wiley.com/10.1111/tbed.13153
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http://dx.doi.org/10.1111/tbed.13153DOI Listing
February 2019
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AAV Vectors for Efficient Gene Delivery to Rodent Hearts.

Methods Mol Biol 2019 ;1950:311-332

Cardiovascular Institute, Icahn School of Medicine at Mount Sinai, New York City, NY, USA.

Currently, gene therapy is one of the most promising fields in biomedicine, with great therapeutic potential for an array of inherited and acquired diseases. Adeno-associated viral (AAV) vectors have emerged as promising tools to deliver selectively a therapeutic payload to target organs, including the heart. In this chapter, we describe the production and quality control of recombinant AAV (rAAV) vectors of the serotype 9, the most cardiotropic AAV serotype when delivered systemically in rodents. Read More

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http://dx.doi.org/10.1007/978-1-4939-9139-6_19DOI Listing
January 2019

The Toxicological Analysis of Ni and Cr in Prescription Food for Special Medical Purposes and Modified Milk Products for Babies in Infancy Available in Pharmacies in Poland.

Biol Trace Elem Res 2019 Feb 19. Epub 2019 Feb 19.

Department of Analytical Chemistry, Faculty of Chemistry, Jagiellonian University in Kraków, Gronostajowa 2, 30-387, Kraków, Poland.

Prescription food for special medical purposes (FSMPs) and modified milk products (MMPs) for babies in the infancy period available in pharmacies are increasingly frequently used for newborns and infants-especially in cases where allergies and food-borne diseases are diagnosed. However, there is a lack of scientific study around the safety assessment of the elemental impurities in these products. Importantly, the allergen Ni and the problematic metal Cr are little considered. Read More

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http://dx.doi.org/10.1007/s12011-019-01667-wDOI Listing
February 2019

Imaging Neuroendocrine Hepatic Metastases Following Y-Radioembolization: Is It Time to Implement Routine Use of PET Molecular/Metabolic Probes?

Cardiovasc Intervent Radiol 2019 Feb 19. Epub 2019 Feb 19.

Nuclear Medicine Unit, "Santa Maria Goretti" Hospital, via Canova, Latina, Italy.

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http://dx.doi.org/10.1007/s00270-019-02186-wDOI Listing
February 2019

The effects of collagen peptides on muscle damage, inflammation and bone turnover following exercise: a randomized, controlled trial.

Amino Acids 2019 Feb 19. Epub 2019 Feb 19.

Institute of Cellular Medicine, Faculty of Medical Sciences, School of Biomedicine, Newcastle University, Newcastle upon Tyne, NE2 4HH, UK.

This study examined whether consuming collagen peptides (CP) before and after strenuous exercise alters markers of muscle damage, inflammation and bone turnover. Using a double-blind, independent group's design, 24 recreationally active males consumed either 20 g day of CP or a placebo control (CON) for 7 days before and 2 days after performing 150 drop jumps. Maximal isometric voluntary contractions, countermovement jumps (CMJ), muscle soreness (200 mm visual analogue scale), pressure pain threshold, Brief Assessment of Mood Adapted (BAM +) and a range of blood markers associated with muscle damage, inflammation and bone turnover C-terminal telopeptide of type 1 collagen (β-CTX) and N-terminal propeptides of type 1 pro-collagen (P1NP) were measured before supplementation (baseline; BL), pre, post, 1. Read More

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http://dx.doi.org/10.1007/s00726-019-02706-5DOI Listing
February 2019

Advance of Seriniquinone Analogues as Melanoma Agents.

ACS Med Chem Lett 2019 Feb 6;10(2):186-190. Epub 2019 Feb 6.

Center for Marine Biotechnology and Biomedicine, Scripps Institution of Oceanography, University of California, San Diego, La Jolla, California 92093-0204, United States.

Seriniquinone, a marine natural product, displayed potent cytotoxicity and selectivity against melanoma cancer cells. This selectivity, combined with a novel mode of action (MOA), prompted studies to translate a pharmacologically relevant lead. Herein, we report on structure-activity relationships (SARs), and provide a strategy to prepare analogues that retain activity and offer an improved water solubility and isomeric purity. Read More

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http://dx.doi.org/10.1021/acsmedchemlett.8b00391DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6378664PMC
February 2019

Crystal structure and substrate interactions of an unusual fungal non-CBM carrying GH26 endo-β-mannanase from Yunnania penicillata.

Sci Rep 2019 Feb 19;9(1):2266. Epub 2019 Feb 19.

Novozymes A/S, Krogshøjvej 36, 2880, Bagsværd, Denmark.

Endo-β(1 → 4)-mannanases (endomannanases) catalyse degradation of β-mannans, an abundant class of plant polysaccharides. This study investigates structural features and substrate binding of YpenMan26A, a non-CBM carrying endomannanase from Yunnania penicillata. Structural and sequence comparisons to other fungal family GH26 endomannanases showed high sequence similarities and conserved binding residues, indicating that fungal GH26 endomannanases accommodate galactopyranosyl units in the -3 and -2 subsites. Read More

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http://dx.doi.org/10.1038/s41598-019-38602-xDOI Listing
February 2019

The flavonoid 4,4'-dimethoxychalcone promotes autophagy-dependent longevity across species.

Nat Commun 2019 Feb 19;10(1):651. Epub 2019 Feb 19.

Institute of Molecular Biosciences, NAWI Graz, University of Graz, Graz, 8010, Austria.

Ageing constitutes the most important risk factor for all major chronic ailments, including malignant, cardiovascular and neurodegenerative diseases. However, behavioural and pharmacological interventions with feasible potential to promote health upon ageing remain rare. Here we report the identification of the flavonoid 4,4'-dimethoxychalcone (DMC) as a natural compound with anti-ageing properties. Read More

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http://dx.doi.org/10.1038/s41467-019-08555-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6381180PMC
February 2019

White shark genome reveals ancient elasmobranch adaptations associated with wound healing and the maintenance of genome stability.

Proc Natl Acad Sci U S A 2019 Feb 19. Epub 2019 Feb 19.

Save Our Seas Shark Research Center, Nova Southeastern University, Dania Beach, FL 33004;

The white shark (; Chondrichthyes, Elasmobranchii) is one of the most publicly recognized marine animals. Here we report the genome sequence of the white shark and comparative evolutionary genomic analyses to the chondrichthyans, whale shark (Elasmobranchii) and elephant shark (Holocephali), as well as various vertebrates. The 4. Read More

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http://dx.doi.org/10.1073/pnas.1819778116DOI Listing
February 2019

Arp2/3-branched actin regulates microtubule acetylation level and affects mitochondrial distribution.

J Cell Sci 2019 Feb 19. Epub 2019 Feb 19.

Institute of Systems Biomedicine, School of Basic Medical Sciences, Peking University Health Science Center, Beijing 100191, China

Actin and microtubule cytoskeletons regulate cell morphology, participate in organelle trafficking and function in response to diverse environmental cues. Precise spatial-temporal coordination between these two cytoskeletons is essential for cells to live and move. Here we report a novel cross-talk between actin and microtubules, in which the branched actin maintains microtubule organization, dynamics and stability by affecting tubulin acetylation level. Read More

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http://dx.doi.org/10.1242/jcs.226506DOI Listing
February 2019

Computational Strategies for Niche-Dependent Cell Conversion to Assist Stem Cell Therapy.

Trends Biotechnol 2019 Feb 16. Epub 2019 Feb 16.

Luxembourg Centre for Systems Biomedicine (LCSB), University of Luxembourg,7 Avenue des Hauts-Fourneaux, Esch-sur-Alzette, L-4362 Luxembourg City, Luxembourg.

The field of regenerative medicine has blossomed in recent decades. However, the ultimate goal of tissue regeneration - replacing damaged or aged cells with healthy functioning cells - still faces a number of challenges. In particular, better understanding of the role of the cellular niche in shaping stem cell phenotype and conversion would aid in improving current protocols for stem cell therapies. Read More

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http://dx.doi.org/10.1016/j.tibtech.2019.01.005DOI Listing
February 2019

The effect of monowave and polywave light-polymerization units on the adhesion of resin cements to zirconia.

J Prosthet Dent 2019 Feb 16. Epub 2019 Feb 16.

Staff Dentist, Department of Prosthodontic, School & Hospital of Stomatology, Wuhan University, Wuhan, PR China. Electronic address:

Statement Of Problem: Although newly developed photoinitiators can make up for the limitations of camphorquinone, their absorption spectra are different. However, little attention has been paid to the compatibility of available wavelength spectra of light-polymerization units (LPUs) and photoinitiators within resin cements.

Purpose: The purpose of this in vitro study was to evaluate the effect of monowave and polywave light-emitting diode (LED) units on the adhesion of dual-polymerizing self-adhesive resin cements to monolithic zirconia. Read More

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http://dx.doi.org/10.1016/j.prosdent.2018.12.010DOI Listing
February 2019

Specific and simultaneous detection of micro RNA 21 and let-7a by rolling circle amplification combined with lateral flow strip.

Anal Chim Acta 2019 May 21;1055:115-125. Epub 2018 Dec 21.

Beijing Key Laboratory for Separation and Analysis in Biomedicine and Pharmaceuticals, School of Life Science, Beijing Institute of Technology, Beijing, China.

Owing to the pivotal function in post transcriptional gene modification, miRNA biomarkers are playing crucial role in tracking and diagnosing various forms of tumors in a short time period. Hence, the need to develop simple, sensitive and specific detection of miRNAs for precise diagnosis arises. This current study is aimed to develop a detecting platform by combining rolling circle amplification with AuNps-based lateral flow strip (LFS-RCA) for simultaneous detection of miRNA 21 and miRNA let-7a. Read More

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http://dx.doi.org/10.1016/j.aca.2018.12.040DOI Listing

Upregulation of Tumor Susceptibility Gene 101 (TSG101) by mechanical stress in podocytes.

Cell Mol Biol (Noisy-le-grand) 2019 Jan 31;65(1):84-88. Epub 2019 Jan 31.

Dept. of Anatomy and Cell Biology, University Medicine Greifswald, D-17487 Greifswald, Germany.

Elevated mechanical stress in glomerular hypertension is thought to damage podocytes, the loss of which leads to development of glomerulosclerosis. Applying cDNA array analysis to mechanically stressed podocytes, we have recently identified TSG101 as a stretch-induced candidate gene among others. TSG101, which is part of the ESCRT-I complex, is involved in multivesicular body (MVB) formation. Read More

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January 2019
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Pertactin-deficient isolates: evidence of increased circulation in Europe, 1998 to 2015.

Euro Surveill 2019 Feb;24(7)

Department of Medical Microbiology, Capital Medical University, Beijing, China.

IntroductionPertussis outbreaks have occurred in several industrialised countries using acellular pertussis vaccines (ACVs) since the 1990s. High prevalence of pertactin (PRN)-deficient isolates has been found in these countries.AimsTo evaluate in Europe: (i) whether proportions of PRN-deficient strains increased in consecutive collections of clinical isolates; (ii) if the frequency of PRN-deficient strains in countries correlated with the time since ACV introduction; (iii) the presence of pertussis toxin (PT)-, filamentous haemagglutinin (FHA)- or fimbriae (Fim)-deficient isolates. Read More

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https://www.eurosurveillance.org/content/10.2807/1560-7917.E
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http://dx.doi.org/10.2807/1560-7917.ES.2019.24.7.1700832DOI Listing
February 2019
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Engineering of leucine-responsive regulatory protein improves spiramycin and bitespiramycin biosynthesis.

Microb Cell Fact 2019 Feb 19;18(1):38. Epub 2019 Feb 19.

NHC Key Laboratory of Biotechnology of Antibiotics, Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences, No. 1 Tian Tan Xi Li, Beijing, 100050, People's Republic of China.

Background: Bitespiramycin (BT) is produced by recombinant spiramycin (SP) producing strain Streptomyces spiramyceticus harboring a heterologous 4″-O-isovaleryltransferase gene (ist). Exogenous L-Leucine (L-Leu) could improve the production of BT. The orf2 gene found from the genomic sequence of S. Read More

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http://dx.doi.org/10.1186/s12934-019-1086-0DOI Listing
February 2019

Setwise comparison: efficient fine-grained rating of movement videos using algorithmic support - a proof of concept study.

Disabil Rehabil 2019 Feb 20:1-7. Epub 2019 Feb 20.

a Department of Neurology , Inselspital, Bern University Hospital, University of Bern , Bern , Switzerland.

Purpose: Clinical ordinal rating scales of movements, e.g., the Expanded Disability Status Scale, have poor intra- and interrater reliability, are insensitive to subtle differences and result in coarse-grained ratings compared to relative comparative rating methods. Read More

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http://dx.doi.org/10.1080/09638288.2018.1563832DOI Listing
February 2019

Tempo-Spatial Pattern of Stepharine Accumulation in Morphogenic Tissues.

Int J Mol Sci 2019 Feb 13;20(4). Epub 2019 Feb 13.

Federal Scientific Center of the East Asia Terrestrial Biodiversity (Institute of Biology and Soil Science), Far Eastern Branch of the Russian Academy of Sciences, 159 Stoletija Str., 690022 Vladivostok, Russia.

Alkaloids attract great attention due to their valuable therapeutic properties. Stepharine, an aporphine alkaloid of plants, exhibits anti-aging, anti-hypertensive, and anti-viral effects. The distribution of aporphine alkaloids in cell cultures, as well as whole plants is unknown, which hampers the development of bioengineering strategies toward enhancing their production. Read More

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http://dx.doi.org/10.3390/ijms20040808DOI Listing
February 2019
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Preexisting Virus-Specific T Lymphocytes-Mediated Enhancement of Adenovirus Infections to Human Blood CD14+ Cells.

Viruses 2019 Feb 13;11(2). Epub 2019 Feb 13.

School of Public Health (Shenzhen), Sun Yat-sen University, Guangdong 518107, China.

Antigen-specific T lymphocytes play a critical role in controlling viral infections. However, we report here that preexisting virus-specific T cell responses also contribute to promoting adenovirus (Ad) infection. Previously, we found that CD14+ monocytes from Ad-seropositive individuals exhibited an increased susceptibility to Ad infection, when compared with that of Ad-seronegative individuals. Read More

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http://dx.doi.org/10.3390/v11020154DOI Listing
February 2019

Long Non Coding RNA H19: A New Player in Hypoxia-Induced Multiple Myeloma Cell Dissemination.

Int J Mol Sci 2019 Feb 13;20(4). Epub 2019 Feb 13.

Department of BioMedicine, Neurosciences and Advanced Diagnostics (Bi.N.D), Via Divisi 83, 90133 Palermo, Italy.

The long non-coding RNA H19 (lncH19) is broadly transcribed in the first stage of development and silenced in most cells of an adult organism; it appears again in several tumors where, through different molecular mediators, promotes cell proliferation, motility and metastases. LncH19 has been associated with hypoxia-inducible factor 1-alpha (HIF-1α) activation and, in some tumors, it has proved to be necessary and required to sustain hypoxic responses. Here we propose to investigate a putative role for the lncH19 in hypoxia induced multiple myeloma (MM) progression. Read More

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http://dx.doi.org/10.3390/ijms20040801DOI Listing
February 2019

Potential of Zerumbone as an Anti-Cancer Agent.

Molecules 2019 Feb 18;24(4). Epub 2019 Feb 18.

Cancer Biology Laboratory, DBT-AIST International Laboratory for Advanced Biomedicine (DAILAB), Department of Biosciences & Bioengineering, Indian Institute of Technology, Guwahati, Assam 781039, India.

Cancer is still a major risk factor to public health globally, causing approximately 9.8 million deaths worldwide in 2018. Despite advances in conventional treatment modalities for cancer treatment, there are still few effective therapies available due to the lack of selectivity, adverse side effects, non-specific toxicities, and tumour recurrence. Read More

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http://dx.doi.org/10.3390/molecules24040734DOI Listing
February 2019

Antioxidant Defense Mechanisms in Erythrocytes and in the Central Nervous System.

Antioxidants (Basel) 2019 Feb 18;8(2). Epub 2019 Feb 18.

Departamento de Morfología y Biología Celular, Facultad de Medicina, Universidad de Oviedo, Asturias 33006, Spain.

Differential antioxidant action is found upon comparison of organ/tissue systems in the human body. In erythrocytes (red blood cells), which transport oxygen and carbon dioxide through the circulatory system, the most important issue is to keep hemoglobin in a functional state that requires maintaining the haem group in ferrous (Fe) state. Conversion of oxidized Fe back into Fe in hemoglobin needs a special mechanism involving a tripeptide glutathione, glucose-6-phosphate dehydrogenase, and glucose and NADPH as suppliers of reducing power. Read More

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http://dx.doi.org/10.3390/antiox8020046DOI Listing
February 2019

IgE Downregulates PTEN through MicroRNA-21-5p and Stimulates Airway Smooth Muscle Cell Remodeling.

Int J Mol Sci 2019 Feb 18;20(4). Epub 2019 Feb 18.

Pneumology & Pulmonary Cell Research, Departments of Internal Medicine & Biomedicine, University & University Hospital Basel, Petersgraben 4, CH-4031 Basel, Switzerland.

The patho-mechanism leading to airway wall remodeling in allergic asthma is not well understood and remodeling is resistant to therapies. This study assessed the effect of immunoglobulin E (IgE) in the absence of allergens on human primary airway smooth muscle cell (ASMC) remodeling in vitro. ASMCs were obtained from five allergic asthma patients and five controls. Read More

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http://dx.doi.org/10.3390/ijms20040875DOI Listing
February 2019

The Neuroprotective Effect of Extracts in Mouse Hippocampus after Pilocarpine-Induced Status Epilepticus.

Int J Mol Sci 2019 Feb 16;20(4). Epub 2019 Feb 16.

Department of Pharmacology, Department of Biomedicine and Health Sciences, Catholic Neuroscience Institute, College of Medicine, The Catholic University of Korea, Seoul 06591, Korea.

(HE), a culinary-medicinal mushroom, has shown therapeutic potential in many brain diseases. However, the role of HE in status epilepticus (SE)-mediated neuronal death and its underlying mechanisms remain unclear. We investigated the neuroprotective effects of HE using a pilocarpine-induced SE model. Read More

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http://dx.doi.org/10.3390/ijms20040859DOI Listing
February 2019

PharmActa: Empowering Patients to Avoid Clinical Significant Drug⁻Herb Interactions.

Medicines (Basel) 2019 Feb 16;6(1). Epub 2019 Feb 16.

Computational Biomedicine Laboratory, Institute of Computer Science, Foundation for Research and Technology-Hellas, Heraklion, GR-70013 Crete, Greece.

Herbal medicinal products (HMPs) are the subject of increasing interest regarding their benefits for health. However, a serious concern is the potential appearance of clinically significant drug⁻herb interactions in patients. This work provides an overview of drug⁻herb interactions and an evaluation of their clinical significance. Read More

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http://dx.doi.org/10.3390/medicines6010026DOI Listing
February 2019

High Sensitivity Flexible Electronic Skin Based on Graphene Film.

Sensors (Basel) 2019 Feb 15;19(4). Epub 2019 Feb 15.

Beijing Key Laboratory of Environmental Science and Engineering, School of Chemical Engineering and the Environment, Beijing Institute of Technology, Beijing 100081, China.

Electronic skin with high sensitivity, rapid response, and long-term stability has great value in robotics, biomedicine, and in other fields. However, electronic skin still has challenges in terms of sensitivity and response time. In order to solve this problem, flexible electronic skin with high sensitivity and the fast response was proposed, based on piezoresistive graphene films. Read More

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http://dx.doi.org/10.3390/s19040794DOI Listing
February 2019

Adrenaline Improves Platelet Reactivity in Ticagrelor-Treated Healthy Volunteers.

Thromb Haemost 2019 Feb 19. Epub 2019 Feb 19.

Department of Molecular and Clinical Medicine, Institute of Medicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.

Background:  Administration of agents that enhance platelet reactivity may reduce the perioperative bleeding risk in patients treated with the adenosine diphosphate (ADP)-receptor antagonist ticagrelor. Adrenaline potentiates ADP-induced aggregation and activation in blood samples from ticagrelor-treated patients, but it has not previously been evaluated in vivo.

Methods:  Ten healthy male subjects were included in an interventional study. Read More

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http://dx.doi.org/10.1055/s-0039-1683461DOI Listing
February 2019

Are subsyndromal manifestations of attention deficit hyperactivity disorder morbid in children? A systematic qualitative review of the literature with meta-analysis.

Psychiatry Res 2019 Feb 3;274:75-90. Epub 2019 Feb 3.

Clinical and Research Programs in Pediatric Psychopharmacology and Adult ADHD, Massachusetts General Hospital, Boston, MA, USA; Department of Psychiatry, Harvard Medical School, Boston, MA, USA. Electronic address:

We conducted a qualitative review (n = 15 manuscripts) and meta-analysis (n = 9 manuscripts) of the extant literature to evaluate the prevalence and morbidity of subthreshold Attention Deficit Hyperactivity Disorder (ADHD). Our qualitative review showed that a sizable minority (mean: 17.7%) of clinically referred and non-referred children met a priori definitions of subthreshold ADHD. Read More

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http://dx.doi.org/10.1016/j.psychres.2019.02.003DOI Listing
February 2019

Discovery of alkoxy benzamide derivatives as novel BPTF bromodomain inhibitors via structure-based virtual screening.

Bioorg Chem 2019 Jan 28;86:494-500. Epub 2019 Jan 28.

State Key Laboratory of Drug Research, CAS Key Laboratory of Receptor Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 555 Zuchongzhi Road, Shanghai 201203, China; University of Chinese Academy of Sciences, 19 Yuquan Road, Beijing 100049, China; School of Life Science and Technology, ShanghaiTech University, 100 Haike Road, Shanghai 201210, China; Open Studio for Druggability Research of Marine Natural Products, Pilot National Laboratory for Marine Science and Technology (Qingdao), 1 Wenhai Road, Aoshanwei, Jimo, Qingdao 266237, China. Electronic address:

Bromodomain PHD finger transcription factor (BPTF), a bromodomain-containing protein, plays a crucial role in the regulation of downstream gene expression through the specific recognition of lysine acetylation on bulk histones. The dysfunction of BPTF is closely involved with the development and progression of many human diseases, especially cancer. Therefore, BPTF bromodomain has become a promising drug target for epigenetic cancer therapy. Read More

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http://dx.doi.org/10.1016/j.bioorg.2019.01.035DOI Listing
January 2019
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S-protected gellan gum: Decisive approach towards mucoadhesive antimicrobial vaginal films.

Int J Biol Macromol 2019 Feb 16. Epub 2019 Feb 16.

Center for Chemistry and Biomedicine, Department of Pharmaceutical Technology, Institute of Pharmacy, University of Innsbruck, Innrain 80/82, 6020 Innsbruck, Austria. Electronic address:

The aim of this study was to synthesize novel polymeric excipients forming mucoadhesive films for treatment of vaginal microbial infections. 2-(2-Amino ethyldisulfanyl) nicotinic acid was conjugated with gellan gum via amide bond formation. The structure of the resulting S-protected gellan gum was confirmed by H NMR. Read More

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http://dx.doi.org/10.1016/j.ijbiomac.2019.02.092DOI Listing
February 2019

Human primary macrophages scavenge aunps and eliminate it through exosomes. a natural shuttling for nanomaterials.

Eur J Pharm Biopharm 2019 Feb 16. Epub 2019 Feb 16.

Department of Oncology and Molecular Medicine, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Rome, Italy. Electronic address:

The use of nanomaterials is increasing but the real risk associated with their use in humans has to be defined. In fact, nanomaterials tend to accumulate in organs over a long period of time and are slowly degraded or eliminated by the body. Exosomes are nanovesicles actively shuttle molecules, including chemical products and metals, through the body. Read More

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http://dx.doi.org/10.1016/j.ejpb.2019.02.014DOI Listing
February 2019

Zebrafish models for attention deficit hyperactivity disorder (ADHD).

Neurosci Biobehav Rev 2019 Feb 16. Epub 2019 Feb 16.

Brain and Behaviour Laboratory, School of Pharmacy and Biomedical Sciences, University of Portsmouth, UK; The International Zebrafish Neuroscience Research Consortium (ZNRC), 309 Palmer Court, Slidell, LA 70458, USA. Electronic address:

Attention deficit hyperactivity disorder (ADHD) is a common, debilitating neurodevelopmental disorder associated with inattentiveness, pathological hyperactivity and impulsivity. Despite the mounting human and animal evidence, the neurological pathways underlying ADHD remain poorly understood. Novel translational model organisms, such as the zebrafish (Danio rerio), are becoming important tools to investigate genetic and pathophysiological mechanisms of various neuropsychiatric disorders. Read More

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http://dx.doi.org/10.1016/j.neubiorev.2019.02.009DOI Listing
February 2019

The knocking down of the oncoprotein Golgi phosphoprotein 3 in T98G cells of glioblastoma multiforme disrupts cell migration by affecting focal adhesion dynamics in a focal adhesion kinase-dependent manner.

PLoS One 2019 19;14(2):e0212321. Epub 2019 Feb 19.

Department of Physiology, School of Medicine, Universidad Austral de Chile, Valdivia, Chile.

Golgi phosphoprotein 3 (GOLPH3) is a conserved protein of the Golgi apparatus that in humans has been implicated in tumorigenesis. However, the precise function of GOLPH3 in malignant transformation is still unknown. Nevertheless, clinicopathological data shows that in more than a dozen kinds of cancer, including gliomas, GOLPH3 could be found overexpressed, which correlates with poor prognosis. Read More

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0212321PLOS
February 2019

Applications of magnetic materials separation in biological nanomedicine.

Electrophoresis 2019 Feb 19. Epub 2019 Feb 19.

Department of Chemistry, College of Sciences, Shanghai University, Shanghai, P. R. China.

As a result of their advantages for superparamagnetic properties, good biocompatibility and high binding capacity, functionalized magnetic materials became widely popular over the past couple of decades, being applied on large scale in various processes of sample preparation for biomedicine. In this work we perform an in-depth review on the current progress in the field of magnetic bead separation, discussing in detail the physical basis of this process, various synthesis methods and surface modification strategies. We place a special focus of attention as well on the latest applications of magnetic polymer microspheres in cell separation, protein purification, immobilized enzyme, nucleic acid separation and extraction of bioactive compounds with low molecular weight. Read More

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http://dx.doi.org/10.1002/elps.201800401DOI Listing
February 2019

Role of phase angle in the evaluation of effect of an immuno-enhanced formula in post-surgical cancer patients: a randomized clinical trial.

Eur Rev Med Pharmacol Sci 2019 Feb;23(3):1322-1334

Section of Clinical Nutrition and Nutrigenomic, Department of Biomedicine and Prevention, University of Rome Tor Vergata, Rome, Italy.

Objective: Neoplastic disease is frequently associated with poor nutritional status or severe malnutrition. Diet and nutritional intervention are becoming increasingly important for prognosis and quality of life in cancer patients. Accessible and repeatable tools for assessing nutritional status with body composition techniques seems to be fundamental. Read More

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http://www.europeanreview.org/article/17027
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http://dx.doi.org/10.26355/eurrev_201902_17027DOI Listing
February 2019
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An atypical aspartic protease modulates lateral root development in Arabidopsis thaliana.

J Exp Bot 2019 Feb 19. Epub 2019 Feb 19.

Institute for Interdisciplinary Research, University of Coimbra, Portugal.

Few atypical aspartic proteases (APs) present in plants have been functionally studied to date despite having been implicated in developmental processes and stress responses, and diverse biochemical properties were reported for these enzymes. Here we characterize a novel atypical AP that we name ATYPICAL ASPARTIC PROTEASE IN ROOTS 1 (ASPR1), denoting its expression in Arabidopsis roots. Recombinant ASPR1 produced by transient expression in N. Read More

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http://dx.doi.org/10.1093/jxb/erz059DOI Listing
February 2019

Decreased Steroid Hormone Receptor Expression in Kawasaki Disease Before IVIG Treatment.

Front Pediatr 2019 4;7. Epub 2019 Feb 4.

Department of Pediatrics, Kaohsiung Chang Gung Memorial Hospital, Chang Gung University College of Medicine, Kaohsiung, Taiwan.

Kawasaki disease (KD) is anacute febrile coronary vasculitis disease in children. In general, this disease can be treated with a single dose of 2 g/kg intravenous immunoglobulin (IVIG). However, the best timing for administering steroid treatment in acute-stage KD is still under debate. Read More

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http://dx.doi.org/10.3389/fped.2019.00007DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6369254PMC
February 2019

Characterization of DNA Methylation Associated Gene Regulatory Networks During Stomach Cancer Progression.

Front Genet 2018 4;9:711. Epub 2019 Feb 4.

Shanghai Center for Systems Biomedicine, Shanghai Jiao Tong University, Shanghai, China.

DNA methylation plays a critical role in tumorigenesis through regulating oncogene activation and tumor suppressor gene silencing. Although extensively analyzed, the implication of DNA methylation in gene regulatory network is less characterized. To address this issue, in this study we performed an integrative analysis on the alteration of DNA methylation patterns and the dynamics of gene regulatory network topology across distinct stages of stomach cancer. Read More

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http://dx.doi.org/10.3389/fgene.2018.00711DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6369581PMC
February 2019

Protective Effects of Aryl Hydrocarbon Receptor Signaling in Celiac Disease Mucosa and in Poly I:C-Induced Small Intestinal Atrophy Mouse Model.

Front Immunol 2019 4;10:91. Epub 2019 Feb 4.

Department of Biomedicine and Prevention, University of Tor Vergata, Rome, Italy.

Aryl hydrocarbon receptor (AhR), a transcription factor activated by a large number of natural and synthetic agents, modulates the activity of immune cells in the gut and represents an important link between the environment and immune-mediated pathologies. In this study, we investigated the role of AhR in celiac disease (CD), a gluten-driven enteropathy. AhR expression was evaluated in intestinal biopsies taken from patients with CD and controls by real-time polymerase chain reaction (PCR), immunohistochemistry and flow cytometry. Read More

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http://dx.doi.org/10.3389/fimmu.2019.00091DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6369162PMC
February 2019
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Extragonadal FSHR Expression and Function-Is It Real?

Front Endocrinol (Lausanne) 2019 4;10:32. Epub 2019 Feb 4.

Institute of Biomedicine, Research Centre for Integrative Physiology and Pharmacology, University of Turku, Turku, Finland.

Expression of the follicle-stimulating hormone receptor (FSHR), besides gonadal tissues, has recently been detected in several extragonadal normal and tumorous tissues, including different types of primary and metastatic cancer and tumor vessel endothelial cells (TVEC). The suggested FSH actions in extragonadal tissues include promotion of angiogenesis, myometrial contractility, skeletal integrity, and adipose tissue accumulation. Non-malignant cells within cancer tissue have been shown to be devoid of FSHR expression, which implies a potential role of FSHR as a diagnostic, prognostic, or even a therapeutic tool. Read More

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http://dx.doi.org/10.3389/fendo.2019.00032DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6369633PMC
February 2019