47,253 results match your criteria Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie[Journal]


Rare case of an oligospermic male with 46,XX/46,XY tetragametic chimerism.

Andrologia 2019 Apr 17:e13290. Epub 2019 Apr 17.

Department of Genetics, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran.

Chimerism, a rare human disorder, is assumed to be the result of an amalgamation of two separate zygotes in a single embryo. Studies have shown that the phenotypic spectrum of chimerism is variable and there is no definite genotype-phenotype correlation in patients with chimerism, therefore a majority of cases might remain undiagnosed. This study aims to investigate the possible mechanism of the chimerism in a 46,XX/46,XY infertile and phenotypically normal male, with 46,XX blood karyotype and normal spermatogenesis. Read More

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http://dx.doi.org/10.1111/and.13290DOI Listing

Elastic properties and intrinsic strength of two-dimensional InSe flakes.

Nanotechnology 2019 Apr 17. Epub 2019 Apr 17.

University of Washington, Seattle, Washington, UNITED STATES.

Mechanical property of two-dimensional (2D) materials is critical for their applications in functional devices as well as for strain engineering. Here, we report the Young's modulus and breaking strength of multilayered InSe, an emerging 2D semiconductors of layered group III chalcogenide. Few-layer InSe flaks were exfoliated from bulk InSe crystal onto Si/SiO2 substrate with micro-fabricated holes, and indentation tests were carried using an Atomic Force Microscopy (AFM) probe. Read More

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http://iopscience.iop.org/article/10.1088/1361-6528/ab1a96
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http://dx.doi.org/10.1088/1361-6528/ab1a96DOI Listing
April 2019
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Transforming Growth Factor-β Signaling in Immunity and Cancer.

Immunity 2019 Apr;50(4):924-940

Cancer Biology and Genetics Program, Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA. Electronic address:

Transforming growth factor (TGF)-β is a crucial enforcer of immune homeostasis and tolerance, inhibiting the expansion and function of many components of the immune system. Perturbations in TGF-β signaling underlie inflammatory diseases and promote tumor emergence. TGF-β is also central to immune suppression within the tumor microenvironment, and recent studies have revealed roles in tumor immune evasion and poor responses to cancer immunotherapy. Read More

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http://dx.doi.org/10.1016/j.immuni.2019.03.024DOI Listing

Functional Network Analysis Reveals the Relevance of SKIIP in the Regulation of Alternative Splicing by p38 SAPK.

Cell Rep 2019 Apr;27(3):847-859.e6

Cell Signaling Research Group, Departament de Ciències Experimentals i de la Salut, Universitat Pompeu Fabra (UPF), 08003 Barcelona, Spain; Institute for Research in Biomedicine (IRB Barcelona), The Barcelona Institute of Science and Technology, Baldiri Reixac, 10, 08028 Barcelona, Spain. Electronic address:

Alternative splicing is a prevalent mechanism of gene regulation that is modulated in response to a wide range of extracellular stimuli. Stress-activated protein kinases (SAPKs) play a key role in controlling several steps of mRNA biogenesis. Here, we show that osmostress has an impact on the regulation of alternative splicing (AS), which is partly mediated through the action of p38 SAPK. Read More

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http://dx.doi.org/10.1016/j.celrep.2019.03.060DOI Listing

IL-38 Ameliorates Skin Inflammation and Limits IL-17 Production from γδ T Cells.

Cell Rep 2019 Apr;27(3):835-846.e5

Institute of Biochemistry I, Faculty of Medicine, Goethe-University Frankfurt, 60590 Frankfurt, Germany. Electronic address:

Interleukin-38 (IL-38) is a cytokine of the IL-1 family with a role in chronic inflammation. However, its main cellular targets and receptors remain obscure. IL-38 is highly expressed in the skin and downregulated in psoriasis patients. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S22111247193042
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http://dx.doi.org/10.1016/j.celrep.2019.03.082DOI Listing
April 2019
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Patterns of support to adolescents related to disability, family situation, harassment and economy.

Child Care Health Dev 2019 Apr 17. Epub 2019 Apr 17.

CHILD Research Group, Department of Nursing Science, School of Health and Welfare, Jönköping University, Jönköping, Sweden.

Introduction: Adolescents need support from family, friends and teachers to increase their involvement in everyday life. Their environment and their own characteristics also influence their ability to participate in an everyday supportive environment.

Aim: The aim of the study was to investigate patterns of support from parents, teachers and very important persons such as peers to the ability of adolescents to participate in everyday life, as well as the importance of interpersonal relations as experienced by the adolescents. Read More

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https://onlinelibrary.wiley.com/doi/abs/10.1111/cch.12675
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http://dx.doi.org/10.1111/cch.12675DOI Listing
April 2019
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Schwann cells ER-associated degradation contributes to myelin maintenance in adult nerves and limits demyelination in CMT1B mice.

PLoS Genet 2019 Apr 17;15(4):e1008069. Epub 2019 Apr 17.

Division of Genetics and Cell Biology, IRCCS San Raffaele Scientific Institute, Milan, Italy.

In the peripheral nervous system (PNS) myelinating Schwann cells synthesize large amounts of myelin protein zero (P0) glycoprotein, an abundant component of peripheral nerve myelin. In humans, mutations in P0 cause the demyelinating Charcot-Marie-Tooth 1B (CMT1B) neuropathy, one of the most diffused genetic disorders of the PNS. We previously showed that several mutations, such as the deletion of serine 63 (P0-S63del), result in misfolding and accumulation of P0 in the endoplasmic reticulum (ER), with activation of the unfolded protein response (UPR). Read More

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http://dx.plos.org/10.1371/journal.pgen.1008069
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http://dx.doi.org/10.1371/journal.pgen.1008069DOI Listing
April 2019
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GLP-1 induces alpha cell proliferation and overrides leptin suppression induced by negative energy balance in vagotomized rats.

J Cell Biochem 2019 Apr 17. Epub 2019 Apr 17.

Endocrine, Cardiovascular & Metabolic Research, Unit for Multidisciplinary Research in Biomedicine (UMIB), University of Porto, Porto, Portugal.

Glucagon-like peptide-1 (GLP-1) influences energy balance by exerting effects on food intake and glucose metabolism, through mechanisms that are partially dependent on the vagal pathway. The aim of this study was to characterize the effects of chronic GLP-1 stimulation on energy homeostasis and glucose metabolism in the absence of vagal innervation Truncal vagotomized (VGX) and sham operated rats (SHAM) received an intraperitoneal GLP-1 infusion (3.5 pmol/kg/min) trough mini-osmotic pumps. Read More

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http://dx.doi.org/10.1002/jcb.28719DOI Listing

Stable gold graphitic nanocapsule doped hydrogels for efficient photothermal antibacterial applications.

Chem Commun (Camb) 2019 Apr 17. Epub 2019 Apr 17.

Molecular Science and Biomedicine Laboratory (MBL), State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, Aptamer Engineering Center of Hunan Province, Hunan University, Changsha, Hunan 410082, China.

Herein, we have fabricated gold nanorod graphitic nanocapsule (AuNR@G) doped poly(vinyl alcohol) (PVA)/chitosan (CS) hydrogels, which possessed highly efficient and stable photothermal antibacterial properties for both Gram-negative E. coli and Gram-positive S. aureus under the irradiation of a near-infrared laser. Read More

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http://dx.doi.org/10.1039/c9cc01933bDOI Listing

Preparation and Characterization of Curcumin-Loaded Polymeric Nanomicelles to Interference with Amyloidogenesis through Glycation Method.

Biotechnol Appl Biochem 2019 Apr 16. Epub 2019 Apr 16.

Bioengineering Research Group, Nanotechnology and Advanced Materials Department, Materials and Energy Research Center (MERC), P.O. Box 14155-4777, Tehran, Iran.

Amyloid fibrils, including β-amyloid (Aβ) fibrils, are protein aggregates that form under certain conditions, associated with neurodegeneration that interfere with neural synaptic transmission resulting in some neural disorders, such as Alzheimer disease. The aim of this study is to inhibit amyloidogenesis by using preparatory polymeric nanomicelles as therapeutic agents and also as nano carriers for curcumin to target Aβ fibrils through the glycation method of Bovine Serum Albumin (BSA) in the presence of Phosphate Buffer Saline (PBS). Polymeric nanomicelles were prepared from Phosphatidylethanolamine-distearoyl methoxypolyethylene glycol conjugates in the presence and absence of Curcumin and then the morphological and structural characteristics of the nanomicelles were characterized in detail. Read More

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https://onlinelibrary.wiley.com/doi/abs/10.1002/bab.1751
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http://dx.doi.org/10.1002/bab.1751DOI Listing
April 2019
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Report on the AAPS Immunogenicity Guidance Forum.

AAPS J 2019 Apr 16;21(4):55. Epub 2019 Apr 16.

Pharmaceutical Consultant, North Potomac, Maryland, 20878, USA.

In September 2018, the American Association of Pharmaceutical Scientists (AAPS) conducted an Annual Guidance Forum on the considerations related to immunogenicity testing for therapeutic protein products. In addition to a broad representation by the pharmaceutical industry, the event included strong representation by leading scientists from the US Food and Drug Administration (FDA). The agency and industry perspectives and updates to the guidance were presented. Read More

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http://dx.doi.org/10.1208/s12248-019-0328-8DOI Listing

Peritonsillar abscess is frequently accompanied by sepsis symptoms.

Eur Arch Otorhinolaryngol 2019 Apr 16. Epub 2019 Apr 16.

Institute of Clinical Medicine, University of Tartu, Tartu, Estonia.

Purpose: To evaluate how many patients with peritonsillar abscess (PTA) fulfill sepsis criteria and if there is any difference in risk factors and treatment results between patients with and without sepsis symptoms. We also aimed to evaluate the utility of several clinical and laboratory markers for diagnosing PTA.

Methods: Study group consisted of 92 patients with PTA undergoing bilateral emergency tonsillectomy. Read More

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http://dx.doi.org/10.1007/s00405-019-05424-6DOI Listing
April 2019
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Patient safety: a new basic science for professional education.

GMS J Med Educ 2019 15;36(2):Doc21. Epub 2019 Mar 15.

University of Verona, Department of Neuroscience, Biomedicine and Movement Sciences, Section of Clinical Psychology, Verona, Italy.

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http://dx.doi.org/10.3205/zma001229DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6446473PMC

Stem cell-associated heterogeneity in Glioblastoma results from intrinsic tumor plasticity shaped by the microenvironment.

Nat Commun 2019 Apr 16;10(1):1787. Epub 2019 Apr 16.

NorLux Neuro-Oncology Laboratory, Department of Oncology, Luxembourg Institute of Health, L-1526, Luxembourg, Luxembourg.

The identity and unique capacity of cancer stem cells (CSC) to drive tumor growth and resistance have been challenged in brain tumors. Here we report that cells expressing CSC-associated cell membrane markers in Glioblastoma (GBM) do not represent a clonal entity defined by distinct functional properties and transcriptomic profiles, but rather a plastic state that most cancer cells can adopt. We show that phenotypic heterogeneity arises from non-hierarchical, reversible state transitions, instructed by the microenvironment and is predictable by mathematical modeling. Read More

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http://dx.doi.org/10.1038/s41467-019-09853-zDOI Listing

Cancer Immunoediting by Innate Lymphoid Cells.

Trends Immunol 2019 Apr 13. Epub 2019 Apr 13.

Laboratory for Immune Cell Systems, RIKEN Center for Integrative Medical Sciences, Yokohama, Japan; Department of Microbiology and Immunology, Keio University School of Medicine, Tokyo, Japan. Electronic address:

The immune system plays a dual role in cancer. It conveys protective immunity but also facilitates malignant progression, either by sculpting tumor immunogenicity or by creating a microenvironment that can stimulate tumor outgrowth or aid in a subsequent metastatic cascade. Innate lymphoid cells (ILCs) embody this functional heterogeneity, although the nature of their responses in cancer has only recently begun to be unveiled. Read More

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http://dx.doi.org/10.1016/j.it.2019.03.004DOI Listing

Discovery of trans-3-(pyridin-3-yl)acrylamide-derived sulfamides as potent nicotinamide phosphoribosyltransferase (NAMPT) inhibitors for the potential treatment of cancer.

Bioorg Med Chem Lett 2019 Apr 8. Epub 2019 Apr 8.

State Key Laboratory of Natural Medicines and Department of Medicinal Chemistry, School of Pharmacy, China Pharmaceutical University, Nanjing 210009, China. Electronic address:

Nicotinamide phosphoribosyltransferase (NAMPT) has emerged as a promising target for the discovery of anticancer drugs. Based on NAMPT inhibitor FK866 that has been advanced into phase II trial, we identified a trans-3-(pyridin-3-yl)acrylamide compound 13 incorporating with a biarylsulfanilamide moiety as a new NAMPT inhibitor. Further structure-activity relationship (SAR) exploration led to additional biarylsulfanilamide-derived compounds with high in vitro NAMPT inhibitory potency and antiproliferative activity. Read More

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http://dx.doi.org/10.1016/j.bmcl.2019.04.013DOI Listing

Mutations in ATP13A2 (PARK9) are associated with an amyotrophic lateral sclerosis-like phenotype, implicating this locus in further phenotypic expansion.

Hum Genomics 2019 Apr 16;13(1):19. Epub 2019 Apr 16.

Center for Human Disease Modeling, Duke University Medical Center, Carmichael Building, 300 North Duke Street, Suite 48-118, Durham, NC, 27701, USA.

Background: Amyotrophic lateral sclerosis [1] is a genetically heterogeneous neurodegenerative disorder, characterized by late-onset degeneration of motor neurons leading to progressive limb and bulbar weakness, as well as of the respiratory muscles, which is the primary cause of disease fatality. To date, over 25 genes have been implicated as causative in ALS with C9orf72, SOD1, FUS, and TARDBP accounting for the majority of genetically positive cases.

Results: We identified two patients of Italian and French ancestry with a clinical diagnosis of juvenile-onset ALS who were mutation-negative in any of the known ALS causative genes. Read More

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http://dx.doi.org/10.1186/s40246-019-0203-9DOI Listing

Respiratory tract virus infections in the elderly with pneumonia.

BMC Geriatr 2019 Apr 16;19(1):111. Epub 2019 Apr 16.

Department of Pediatrics and Adolescent Medicine, University of Turku and Turku University Hospital, PO Box 52, 20520, Turku, Finland.

Background: In children suffering from severe lower airway illnesses, respiratory virus detection has given good prognostic information, but such reports in the elderly are scarce. Therefore, our aim was to study whether the detection of nasopharyngeal viral pathogens and conventional inflammatory markers in the frail elderly correlate to the presence, signs and symptoms or prognosis of radiographically-verified pneumonia.

Methods: Consecutive episodes of hospital care of patients 65 years and older with respiratory symptoms (N = 382) were prospectively studied as a cohort. Read More

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http://dx.doi.org/10.1186/s12877-019-1125-zDOI Listing

The Induced Smoothed lasso: A practical framework for hypothesis testing in high dimensional regression.

Stat Methods Med Res 2019 Apr 16:962280219842890. Epub 2019 Apr 16.

2 Dipartimento di Scienze Economiche, Aziendali e Statistiche, Università degli Studi di Palermo, Palermo, Italy.

This paper focuses on hypothesis testing in lasso regression, when one is interested in judging statistical significance for the regression coefficients in the regression equation involving a lot of covariates. To get reliable p-values, we propose a new lasso-type estimator relying on the idea of induced smoothing which allows to obtain appropriate covariance matrix and Wald statistic relatively easily. Some simulation experiments reveal that our approach exhibits good performance when contrasted with the recent inferential tools in the lasso framework. Read More

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http://dx.doi.org/10.1177/0962280219842890DOI Listing

Anthracycline-based consolidation may determine outcome of post-consolidation immunotherapy in AML.

Leuk Lymphoma 2019 Apr 17:1-8. Epub 2019 Apr 17.

a TIMM Laboratory , Sahlgrenska Cancer Center, Department of Infectious Diseases, Institute of Biomedicine, Sahlgrenska Academy, University of Gothenburg , Gothenburg , Sweden.

Consolidation chemotherapy in acute myeloid leukemia (AML) aims at eradicating residual leukemic cells and mostly comprises high-dose cytarabine with or without the addition of anthracyclines, including daunorubicin. Immunogenic cell death (ICD) may contribute to the efficacy of anthracyclines in solid cancer, but the impact of ICD in AML is only partly explored. We assessed aspects of ICD, as reflected by calreticulin expression, in primary human AML blasts and observed induction of surface calreticulin upon exposure to daunorubicin but not to cytarabine. Read More

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http://dx.doi.org/10.1080/10428194.2019.1599110DOI Listing

Chest Computed Tomography Findings and Validation of Clinical Criteria of Stroke Associated Pneumonia.

J Stroke 2019 Apr 17. Epub 2019 Apr 17.

Stroke Research Program, Institute of Biomedicine of Seville (IBIS)/ University Hospital Virgen del Rocio/CSIC/University of Seville, Seville, Spain.

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http://dx.doi.org/10.5853/jos.2018.03251DOI Listing

Extracellular Vesicles as Biological Shuttles for Targeted Therapies.

Int J Mol Sci 2019 Apr 15;20(8). Epub 2019 Apr 15.

Department of BioMedicine, Neuroscience and Advanced Diagnostics (Bi.N.D), Section of Biology and Genetics, University of Palermo, 90133 Palermo, Italy.

The development of effective nanosystems for drug delivery represents a key challenge for the improvement of most current anticancer therapies. Recent progress in the understanding of structure and function of extracellular vesicles (EVs)-specialized membrane-bound nanocarriers for intercellular communication-suggests that they might also serve as optimal delivery systems of therapeutics. In addition to carrying proteins, lipids, DNA and different forms of RNAs, EVs can be engineered to deliver specific bioactive molecules to target cells. Read More

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http://dx.doi.org/10.3390/ijms20081848DOI Listing

HDAC6 Suppresses Let-7i-5p to Elicit TSP1/CD47-mediated Anti-tumorigenesis and Phagocytosis of Hepatocellular Carcinoma.

Hepatology 2019 Apr 16. Epub 2019 Apr 16.

Department of Pathology, College of Medicine, The Catholic University of Korea, 222 Banpo-daero, Seocho-gu, Seoul, 06591, Republic of Korea.

Histone deacetylase 6 (HDAC6) uniquely endows as tumor suppressor in hepatocellular carcinogenesis, but the underlying mechanisms leading to tumor suppression are not fully understood. To identify comprehensive microRNAs (miRNAs) regulated by HDAC6 in hepatocellular carcinogenesis, differential miRNA expression analysis of HDAC6-transfected Hep3B cells was performed. Using integrative analyses of publicly available transcriptome data and miRNA target prediction, we selected 5 candidate miRNAs and, through in vitro functional validation, showed that let-7i-5p specifically suppressed thrombospondin-1 (TSP1) in hepatocellular carcinoma (HCC). Read More

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http://dx.doi.org/10.1002/hep.30657DOI Listing
April 2019
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Genes associated with Parkinson's disease respond to increasing polychlorinated biphenyl levels in the blood of healthy females.

Environ Pollut 2019 Apr 8;250:107-117. Epub 2019 Apr 8.

Department of Toxicogenomics, Maastricht University, P.O. Box 616, 6200 MD, Maastricht, The Netherlands, 6229, ER Maastricht, the Netherlands.

Polychlorinated biphenyls (PCBs) are a class of widespread environmental pollutants, commonly found in human blood, that have been suggested to be linked to the occurrence of sporadic Parkinson's disease (PD). It has been reported that some non-coplanar PCBs accumulate in the brains of female PD patients. To improve our understanding of the association between PCB exposure and PD risk we have applied whole transcriptome gene expression analysis in blood cells from 594 PCB-exposed subjects (369 female, 225 male). Read More

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http://dx.doi.org/10.1016/j.envpol.2019.04.005DOI Listing

The brief assessment of cognition in affective disorders: Normative data for the Italian population.

J Affect Disord 2019 Apr 8;252:245-252. Epub 2019 Apr 8.

Department of Neurosciences and Mental Health, Foundation IRCCS Ca' Granda Ospedale Maggiore Policlinico, University of Milan, Milan, Italy; Department of Psychiatry and Behavioural Sciences, University of Texas at Houston, USA. Electronic address:

Background: To date there are no validated tests in Italian to assess cognitive functions in Bipolar Disorder. Therefore, this study aimed to provide normative data for the Italian version of the Brief Assessment of Cognition in Affective Disorders (BAC-A), a battery targeting neuro- and affective-cognition in affective disorders.

Methods: Data were collected from 228 healthy participants (age range: 18-67; mean age: 34. Read More

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http://dx.doi.org/10.1016/j.jad.2019.04.063DOI Listing

Forensic classification of black inkjet prints using Fourier transform near-infrared spectroscopy and Linear Discriminant Analysis.

Forensic Sci Int 2019 Mar 30;299:128-134. Epub 2019 Mar 30.

Leopold-Franzens University, Institute of Analytical Chemistry and Radiochemistry, CCB - Center for Chemistry and Biomedicine, Innrain 80/82, 6020 Innsbruck, Austria. Electronic address:

This work presents a study regarding the forensic discrimination of black inkjet-printed documents in question. Nondestructive Fourier transform near-infrared (FT-NIR) spectroscopy in combination with supervised classification method Discriminant Analysis (DA); Linear Discriminant Analysis (LDA) and Quadratic Discriminant Analysis (QDA) were utilized to investigate 22 different prints of the three most sold office printer brands. The spectra were acquired using the FT-NIR spectrometer NIRFlex N-500 (Büchi Labortechnik AG, Flawil, Switzerland) with the Fiber Optic Solids measuring cell in the spectral region of 10,000-4000 cm. Read More

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http://dx.doi.org/10.1016/j.forsciint.2019.03.041DOI Listing

Tripterygium agents for the treatment of atopic eczema: A Bayesian analysis of randomized controlled trials.

Phytomedicine 2019 Apr 4;59:152914. Epub 2019 Apr 4.

Department of Dermatology, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China; Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China; Institute of Dermatology, Shanghai Academy of Traditional Chinese Medicine, Shanghai 201203, China. Electronic address:

Background: Atopic eczema is a common and recrudescent skin disorder. Tripterygium agents (TA), extracted from Tripterygium wilfordii hook F, a traditional Chinese medicine, have been used as a supplemental therapy for treating eczema empirically in recent years.

Purpose: To investigate the efficacy and safety of TA for treating atopic eczema. Read More

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http://dx.doi.org/10.1016/j.phymed.2019.152914DOI Listing

Editorial: Biology and clinical relevance of Hydroxysteroid (17beta) dehydrogenase enzymes.

Authors:
Matti Poutanen

Mol Cell Endocrinol 2019 Apr 13. Epub 2019 Apr 13.

Institute of Biomedicine, Research Centre for Integrative Physiology and Pharmacology, University of Turku, Finland; Institute of Medicine, The Sahlgrenska Academy, University of Gothenburg, Sweden.

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http://dx.doi.org/10.1016/j.mce.2019.04.008DOI Listing

Fluid platelet-rich fibrin stimulates greater dermal skin fibroblast cell migration, proliferation, and collagen synthesis when compared to platelet-rich plasma.

J Cosmet Dermatol 2019 Apr 16. Epub 2019 Apr 16.

Department of Periodontology, University of Bern, Bern, Switzerland.

Background: Regenerative therapies in the field of facial aesthetics have become a growing field of interest with many recent advancements made over the past decade to meet the growing worldwide demand. While first versions of platelet-derived concentrates were formulated with anticoagulants (PRP), recent modifications to centrifugation speeds and times have permitted the development of a liquid platelet-rich fibrin (fluid-PRF) without use of anticoagulants.

Objective: To compare this entirely natural platelet concentrate (fluid-PRF) to formally utilized PRP on skin cell behavior and regeneration. Read More

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http://dx.doi.org/10.1111/jocd.12955DOI Listing

Improvement in serological diagnosis of pertussis by external quality assessment.

J Med Microbiol 2019 Apr 16. Epub 2019 Apr 16.

5 Department of Medical Microbiology, Capital Medical University, No.10 Xi Tou Tiao, You'an Men Wai, Feng Tai District, 100069 Beijing, PR China.

Purpose: Serological analysis is an essential tool for the diagnosis of pertussis or whooping cough, disease surveillance and the evaluation of vaccine effectiveness against Bordetella pertussis. Accurate measurement of anti-pertussis toxin (anti-PT) IgG antibody levels in sera is essential. These measurements are usually performed using immunological methods such as ELISA and multiplex immunoassays. Read More

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http://dx.doi.org/10.1099/jmm.0.000926DOI Listing

Oxidation reduction potential: a new biomarker of male infertility.

Panminerva Med 2019 Jun;61(2):108-117

American Center for Reproductive Medicine, Department of Urology, Cleveland Clinic, Cleveland, OH, USA -

Oxidative stress is considered a major etiology for male infertility, more specifically idiopathic infertility. The causes of seminal oxidative stress can be intrinsic, such as varicocele or due to the presence of active leukocytes and immature germ cells. Reported external causes are smoking, alcohol or exposure to environmental toxins. Read More

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https://www.minervamedica.it/index2.php?show=R41Y2019N02A010
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http://dx.doi.org/10.23736/S0031-0808.18.03529-2DOI Listing
June 2019
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2.276 Impact Factor

Recent advances in PET probes for hepatocellular carcinoma characterization.

Expert Rev Med Devices 2019 Apr 16. Epub 2019 Apr 16.

a Department of Nuclear Medicine , Santa Maria Goretti Hospital , Via Canova 3, 04100 Latina , Italy.

Introduction: Positron Emission Tomography (PET) with F-fluorodeoxyglucose (FDG) presents some limitations for imaging of hepatocellular carcinoma (HCC), the most common primary hepatic malignancy. Areas covered: The authors discuss the accuracy and limitations of FDG for HCC detection. Then, authors examine the recent advances in PET tracers other than FDG for the biological and prognostic characterization of HCC such as C-acetate, C-choline and its F-labeled derivatives. Read More

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https://www.tandfonline.com/doi/full/10.1080/17434440.2019.1
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http://dx.doi.org/10.1080/17434440.2019.1608817DOI Listing
April 2019
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Identification of the potential key genes for adipogenesis from human mesenchymal stem cells by RNA-Seq.

J Cell Physiol 2019 Apr 15. Epub 2019 Apr 15.

Jiangxi Provincial Key Laboratory of Systems Biomedicine, Jiujiang University, Jiujiang, China.

Adipogenesis, a physiological process initiated with the committed preadipocytes expressing adipocyte-specific genes and terminated in mature, differentiated and functional adipocytes, mainly involved with energy homeostasis. Abnormal distribution-changes and dysfunctions in adipogenesis may lead to complex physiopathological disorders. However, it remains unclear for the key players working for the whole complex differentiating process of adipogenesis. Read More

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http://dx.doi.org/10.1002/jcp.28621DOI Listing

Label-free diagnosis of lung cancer with tissue-slice surface-enhanced Raman spectroscopy and statistical analysis.

Lasers Med Sci 2019 Apr 13. Epub 2019 Apr 13.

Shandong Province Key Laboratory of Medical Physics and Image Processing Technology, School of Physics and Electronics, Shandong Normal University, Jinan, 250014, China.

Despite the rapid development of medical science, the diagnosis of lung cancer is still quite challenging. Due to the ultrahigh detection sensitivity of surface-enhanced Raman spectroscopy (SERS), SERS has a broad application prospect in biomedicine, especially in the field of tumor blood detection. Although Raman spectroscopy can diagnose lung cancer through tissue slices, its weak cross sections are problematic. Read More

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http://dx.doi.org/10.1007/s10103-019-02781-wDOI Listing

Systemic LPS induces toll-like receptor 3 (TLR3) expression and apoptosis in testicular mouse tissue.

Cell Tissue Res 2019 Apr 15. Epub 2019 Apr 15.

Clinic of Urology and Pediatric Urology, Philipps-University Marburg, Frankfurt, Germany.

It is well known that sepsis and inflammation reduce male fertility. Within the testis, toll-like receptor 3 (TLR3) is constitutively expressed and recognizes double-stranded RNA (dsRNA) from viruses, degraded bacteria, damaged tissues and necrotic cells. To characterize the potential role of TLR3 in response to testicular infections, its expression and downstream signaling were investigated upon challenge with lipopolysaccharides (LPS) in two mouse strains that differ in their immuno-competence regarding T cell-regulated immunity. Read More

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http://dx.doi.org/10.1007/s00441-019-03022-wDOI Listing

Islet inflammation in type 2 diabetes.

Semin Immunopathol 2019 Apr 15. Epub 2019 Apr 15.

Endocrinology, Diabetes and Metabolism, University Hospital of Basel, 4031, Basel, Switzerland.

Metabolic diseases including type 2 diabetes are associated with meta-inflammation. β-Cell failure is a major component of the pathogenesis of type 2 diabetes. It is now well established that increased numbers of innate immune cells, cytokines, and chemokines have detrimental effects on islets in these chronic conditions. Read More

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http://dx.doi.org/10.1007/s00281-019-00745-4DOI Listing
April 2019
2 Reads

Transcription factor Pf-Rel regulates expression of matrix protein genes Prismalin-14 and MSI60 in the pearl oyster Pinctada fucata.

Acta Biochim Biophys Sin (Shanghai) 2019 Apr 16. Epub 2019 Apr 16.

Protein Science Laboratory of the Ministry of Education, School of Life Science, Tsinghua University, Beijing, China.

Molluscan shell is a biomineral that consists of a highly organized calcium carbonate composite. Organisms mainly use matrix proteins to elaborately control the biomineralization process, but knowledge of their regulatory mechanisms is limited. The transcription factor Pf-Rel, which belongs to the Rel/nuclear factor-κB family, was shown to regulate transcription at the Nacrein promoter in the pearl oyster Pinctada fucata. Read More

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http://dx.doi.org/10.1093/abbs/gmz031DOI Listing

CONTEMPORARY RADIATION DOSES IN INTERVENTIONAL CARDIOLOGY: A NATIONWIDE STUDY OF PATIENT DOSES IN FINLAND.

Radiat Prot Dosimetry 2019 Apr 16. Epub 2019 Apr 16.

Department of Medical Physics, Turku University Hospital, Turku, Finland.

The amount of interventional procedures such as percutaneous coronary intervention (PCI), transcatheter aortic valve implantation (TAVI), pacemaker implantation (PI) and ablations has increased within the previous decade. Simultaneously, novel fluoroscopy mainframes enable lower radiation doses for patients and operators. Therefore, there is a need to update the existing diagnostic reference levels (DRLs) and propose new ones for common or recently introduced procedures. Read More

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http://dx.doi.org/10.1093/rpd/ncz041DOI Listing

Deep learning opens new horizons in personalized medicine.

Biomed Rep 2019 Apr 13;10(4):215-217. Epub 2019 Mar 13.

Computational Biomedicine Laboratory (CBML), Institute of Computer Science (ICS), Foundation for Research and Technology Hellas (FORTH), 70013 Heraklion, Greece.

Although the idea of the personalization of patient care dates back to the time of Hippocrates, recent advances in diagnostic medical imaging and molecular medicine are gradually transforming healthcare services, by offering information and diagnostic tools enabling individualized patient management. Facilitating personalized / precision medicine requires taking into account multiple heterogenous parameters, such as sociodemographics, gene variability, environmental and lifestyle factors. Therefore, one of the most critical challenges in personalized medicine is the need to transform large, multi-modal data into decision support tools, capable of bridging the translational gap to the clinical setting. Read More

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http://www.spandidos-publications.com/10.3892/br.2019.1199
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http://dx.doi.org/10.3892/br.2019.1199DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6439426PMC
April 2019
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Optoacoustic mesoscopy for biomedicine.

Nat Biomed Eng 2019 Apr 15. Epub 2019 Apr 15.

Institute of Biological and Medical Imaging, Helmholtz Zentrum München, Munich, Germany.

Fuelled by innovation, optical microscopy plays a critical role in the life sciences and medicine, from basic discovery to clinical diagnostics. However, optical microscopy is limited by typical penetration depths of a few hundred micrometres for in vivo interrogations in the visible spectrum. Optoacoustic microscopy complements optical microscopy by imaging the absorption of light, but it is similarly limited by penetration depth. Read More

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http://dx.doi.org/10.1038/s41551-019-0377-4DOI Listing

Structural basis of specific H2A K13/K15 ubiquitination by RNF168.

Nat Commun 2019 Apr 15;10(1):1751. Epub 2019 Apr 15.

Department of Macromolecular Biochemistry, Leiden Institute of Chemistry, Leiden University, P.O. Box 9502, 2300 RA, Leiden, the Netherlands.

Ubiquitination of chromatin by modification of histone H2A is a critical step in both regulation of DNA repair and regulation of cell fate. These very different outcomes depend on the selective modification of distinct lysine residues in H2A, each by a specific E3 ligase. While polycomb PRC1 complexes modify K119, resulting in gene silencing, the E3 ligase RNF168 modifies K13/15, which is a key event in the response to DNA double-strand breaks. Read More

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http://www.nature.com/articles/s41467-019-09756-z
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http://dx.doi.org/10.1038/s41467-019-09756-zDOI Listing
April 2019
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Competition in the chaperone-client network subordinates cell-cycle entry to growth and stress.

Life Sci Alliance 2019 Apr 15;2(2). Epub 2019 Apr 15.

Molecular Biology Institute of Barcelona, CSIC, Catalonia, Spain

The precise coordination of growth and proliferation has a universal prevalence in cell homeostasis. As a prominent property, cell size is modulated by the coordination between these processes in bacterial, yeast, and mammalian cells, but the underlying molecular mechanisms are largely unknown. Here, we show that multifunctional chaperone systems play a concerted and limiting role in cell-cycle entry, specifically driving nuclear accumulation of the G1 Cdk-cyclin complex. Read More

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http://dx.doi.org/10.26508/lsa.201800277DOI Listing

Comparable Efficacy and Better Safety of Double β-Lactam Combination Therapy versus β-Lactam plus Aminoglycoside in Gram-negatives: A Meta-analysis of Randomized, Controlled Trials.

Antimicrob Agents Chemother 2019 Apr 15. Epub 2019 Apr 15.

Departments of Pharmaceutics and Pharmacotherapy & Translational Research, College of Pharmacy, University of Florida, Orlando, FL

There is a great need for efficacious therapies against Gram-negative bacteria. Double β-lactam combinations (DBL) are relatively safe and preclinical data are promising; however, their clinical role has not been well defined. We conducted a meta-analysis of the clinical and microbiological efficacy of DBL compared to β-lactam plus aminoglycoside combinations (BLAG). Read More

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http://dx.doi.org/10.1128/AAC.00425-19DOI Listing

Role of Sialic Acid in Adhesion to Pig Colonic Mucins.

Infect Immun 2019 Apr 15. Epub 2019 Apr 15.

Department of Medical Biochemistry and Cell biology, Institute of Biomedicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden

Infection with results in mucoid hemorrhagic diarrhea. This pathogen is associated with the colonic mucus layer, mainly composed of mucins. Infection regulates mucin -glycosylation in the colon and increases mucin secretion as well as binding sites on mucins. Read More

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http://dx.doi.org/10.1128/IAI.00889-18DOI Listing

Siglec-9 regulates an effector memory CD8+ T-cell subset that congregates in the melanoma tumor microenvironment.

Cancer Immunol Res 2019 Apr 15. Epub 2019 Apr 15.

Institute of pharmacology, University of Bern

Emerging evidence suggests an immunosuppressive role of altered tumor glycosylation due to downregulation of innate immune responses via immunoregulatory Siglecs. In contrast, human T cells, a major anti-cancer effector cell, only rarely express Siglecs. However, here, we report that the majority of intratumoral, but not peripheral blood, cytotoxic CD8+ T cells expressed Siglec-9 in melanoma. Read More

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http://dx.doi.org/10.1158/2326-6066.CIR-18-0505DOI Listing

Pharmacological PPARβ/δ activation upregulates VLDLR in hepatocytes.

Clin Investig Arterioscler 2019 Apr 13. Epub 2019 Apr 13.

Department of Pharmacology, Toxicology and Therapeutic Chemistry, Faculty of Pharmacy and Food Sciences, University of Barcelona, Institute of Biomedicine of the University of Barcelona (IBUB), Barcelona, Spain; Spanish Biomedical Research Center in Diabetes and Associated Metabolic Diseases (CIBERDEM), Instituto de Salud Carlos III, Spain; Research Institute, Hospital Sant Joan de Déu, Barcelona, Spain. Electronic address:

The very low-density lipoprotein receptor (VLDLR) plays an important function in the control of serum triglycerides and in the development of non-alcoholic fatty liver disease (NAFLD). In this study, we investigated the role of peroxisome proliferator-activated receptor (PPAR)β/δ activation in hepatic VLDLR regulation. Treatment of mice fed a high-fat diet with the PPARβ/δ agonist GW501516 increased the hepatic expression of Vldlr. Read More

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http://dx.doi.org/10.1016/j.arteri.2019.01.004DOI Listing

Immune checkpoint molecule herpes virus entry mediator is overexpressed and associated with poor prognosis in human glioblastoma.

EBioMedicine 2019 Apr 12. Epub 2019 Apr 12.

Department of Neurosurgery, Qilu Hospital of Shandong University and Brain Science Research Institute, Shandong University, Key Laboratory of Brain Functional Remodeling, Shandong, 107# Wenhua Xi Road, Jinan 250012, China. Electronic address:

Background: Dysregulation of immune checkpoint molecules leads to immune evasion in human tumours but has become a viable target for tumour therapy. Here, we examined expression of Herpes virus entry mediator (HVEM), an immune checkpoint molecule, in human glioblastoma (GBM) to assess its potential as a molecular target for treatment.

Methods: Molecular and clinical data from publicly available genomic databases containing WHO grade II-IV human glioma cases (n = 1866) were analyzed. Read More

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http://dx.doi.org/10.1016/j.ebiom.2019.04.002DOI Listing

Direct and rapid characterization of illicit drugs in adulterated samples using thermal desorption electrospray ionization mass spectrometry.

J Food Drug Anal 2019 Apr 8;27(2):451-459. Epub 2019 Jan 8.

Department of Chemistry, National Sun Yat-Sen University, Kaohsiung, Taiwan; Department of Medicinal and Applied Chemistry, Kaohsiung Medical University, Kaohsiung, Taiwan; Research Center for Environmental Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan; Rapid Screening Research Center for Toxicology and Biomedicine, National Sun Yat-Sen University, Kaohsiung, Taiwan. Electronic address:

Foods and drinks have been adulterated with illicit drugs to facilitate criminal activities. Unfortunately, conventional analytical methods are incapable of rapidly characterizing these drugs in samples, as serious interferences from sample matrices must be removed through tedious and time-consuming pretreatment. Ambient ionization mass spectrometry (AMS) generally does not require sample pretreatment and is thus a suitable tool for directly and rapidly detecting illicit drugs in samples in different physical states. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S10219498183017
Publisher Site
http://dx.doi.org/10.1016/j.jfda.2018.12.005DOI Listing
April 2019
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