47,302 results match your criteria Biomedicine & Pharmacotherapy[Journal]


Age-dependent variations in the expression of myosin isoforms and myogenic factors during the involution of the proximal sesamoidean ligament of sheep.

Res Vet Sci 2019 Apr 11;124:270-279. Epub 2019 Apr 11.

Department of Comparative Biomedicine and Food Science, Università di Padova, Italy. Electronic address:

In ungulates the stability of the fetlock joint is dependent on several muscles, which are exposed to high stress and strain. Among those muscles, the proximal sesamoidean ligament or PSL (also known as the suspensory ligament or Ruini's elasto-tendinous organ) is organized at birth in layers of muscle fibres alternated with abundant tendinous tissue that, during the postnatal development, becomes the predominant tissue. In this study we analysed the PSL of the sheep at the age of 1, 30 and 180 days and determined the expression of several genes which either (a) are markers of muscle fibre growth and maturation, or (b) play a role as signal molecules. Read More

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http://dx.doi.org/10.1016/j.rvsc.2019.04.006DOI Listing

Honokiol for cancer therapeutics: A traditional medicine that can modulate multiple oncogenic targets.

Pharmacol Res 2019 Apr 16. Epub 2019 Apr 16.

Cancer Biology Laboratory and DBT-AIST International Laboratory for Advanced Biomedicine (DAILAB) Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Assam, 781039, India. Electronic address:

In spite of billions of dollars expended on cancer research every year, the incidence rate and the mortality rate due to this widespread disease has increased drastically over the last few decades. Recent reports from the World Health Organization advocate that overall global cancer burden and deaths due to cancer are expected to double by the next decade. Synthetic drugs developed as chemotherapeutics have repeatedly shown adverse side effects and development of chemoresistance. Read More

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http://dx.doi.org/10.1016/j.phrs.2019.04.004DOI Listing
April 2019
1 Read

Corrigendum to Stage-specific metabolic features of differentiating neurons: Implications for toxicant sensitivity Toxicol Appl Pharmacol, 354 (2018) 64-80.

Toxicol Appl Pharmacol 2019 Apr 16. Epub 2019 Apr 16.

In Vitro Toxicology and Biomedicine, Dept Inaugurated by the Doerenkamp-Zbinden Foundation, University of Konstanz, 78457 Konstanz, Germany; CAAT-Europe, University of Konstanz, Konstanz 78457, Germany. Electronic address:

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http://dx.doi.org/10.1016/j.taap.2019.04.010DOI Listing

miR‑145‑5p is associated with pathological complete response to neoadjuvant chemotherapy and impairs cell proliferation by targeting TGFβR2 in breast cancer.

Oncol Rep 2019 Apr 5. Epub 2019 Apr 5.

Genomics Sciences Program, Autonomous University of Mexico City, Mexico City 03100, Mexico.

Cancer patients who better benefit from neoadjuvant chemotherapy (NeoCh) are those who achieve a successful pathological complete response (pCR) represented by the absence of residual disease. Unfortunately, no highly sensitive and specific tumor biomarkers for predicting the clinical response to NeoCh have yet been defined. The aim of the present study was to ascertain whether miR‑145‑5p could discriminate between pCR and no‑pCR in triple‑negative breast cancer patients that received a cisplatin/doxorubicin‑based neoadjuvant treatment. Read More

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http://dx.doi.org/10.3892/or.2019.7102DOI Listing

Metal Binding Antimicrobial Peptides in Nanoparticle Bio-functionalization: New Heights in Drug Delivery and Therapy.

Probiotics Antimicrob Proteins 2019 Apr 18. Epub 2019 Apr 18.

Biochemistry Department, Faculty of Science, Ege University, 35100, Bornova, Izmir, Turkey.

Peptides are considered very important due to the diversity expressed through their amino acid sequence, structure variation, large spectrum, and their essential role in biological systems. Antimicrobial peptides (AMPs) emerged as a potent tool in therapy owing to their antimicrobial properties but also their ability to trespass the membranes, specificity, and low toxicity. They comprise a variety of peptides from which specific amino acid-rich peptides are of interest to the current review due to their features in metal interaction and cell penetration. Read More

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http://link.springer.com/10.1007/s12602-019-09546-5
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http://dx.doi.org/10.1007/s12602-019-09546-5DOI Listing
April 2019
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Early Hearing Loss upon Disruption of Slc4a10 in C57BL/6 Mice.

J Assoc Res Otolaryngol 2019 Apr 18. Epub 2019 Apr 18.

Institute of Human Genetics, Jena University Hospital, Friedrich Schiller Universität, Am Klinikum 1, 07747, Jena, Germany.

The unique composition of the endolymph with a high extracellular K concentration is essential for sensory transduction in the inner ear. It is secreted by a specialized epithelium, the stria vascularis, that is connected to the fibrocyte meshwork of the spiral ligament in the lateral wall of the cochlea via gap junctions. In this study, we show that in mice the expression of the bicarbonate transporter Slc4a10/Ncbe/Nbcn2 in spiral ligament fibrocytes starts shortly before hearing onset. Read More

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http://link.springer.com/10.1007/s10162-019-00719-1
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http://dx.doi.org/10.1007/s10162-019-00719-1DOI Listing
April 2019
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Critical role of spectrin in hearing development and deafness.

Sci Adv 2019 Apr 17;5(4):eaav7803. Epub 2019 Apr 17.

iHuman Institute, ShanghaiTech University, Shanghai, China.

Inner ear hair cells (HCs) detect sound through the deflection of mechanosensory stereocilia. Stereocilia are inserted into the cuticular plate of HCs by parallel actin rootlets, where they convert sound-induced mechanical vibrations into electrical signals. The molecules that support these rootlets and enable them to withstand constant mechanical stresses underpin our ability to hear. Read More

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http://advances.sciencemag.org/lookup/doi/10.1126/sciadv.aav
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http://dx.doi.org/10.1126/sciadv.aav7803DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6469942PMC
April 2019
1 Read

CACNA2D3 Enhances the Chemosensitivity of Esophageal Squamous Cell Carcinoma to Cisplatin via Inducing Ca-Mediated Apoptosis and Suppressing PI3K/Akt Pathways.

Front Oncol 2019 2;9:185. Epub 2019 Apr 2.

Department of Cell Biology, Institute of Biomedicine, Jinan University, Guangzhou, China.

Resistance to platinum-based combination chemotherapy is the main cause of poor prognosis in patients with advanced esophageal squamous cell carcinoma (ESCC). Previously, we showed that CACNA2D3 (voltage-dependent subunit alpha 2 delta 3 of a calcium channel complex) was significantly downregulated and functioned as a tumor suppressor in ESCC, but its role in the chemosensitivity of ESCC to cisplatin remained unknown. Here, we found that the expression of CACNA2D3 was significantly associated with poor platinum response in ESCC patients from the Gene Expression Omnibus database. Read More

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http://dx.doi.org/10.3389/fonc.2019.00185DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6454090PMC

Feto-Maternal Microchimerism: The Pre-eclampsia Conundrum.

Front Immunol 2019 29;10:659. Epub 2019 Mar 29.

Department of Biomedicine, University Hospital Basel, Basel, Switzerland.

Feto-maternal microchimerism (FMM) involves bidirectional cross-placental trafficking during pregnancy, leading to a micro-chimeric state that can persist for decades. In this manner a pregnant woman will harbor cells from her mother, as well as, cells from her child. Historically, eclampsia, a severe disorder of pregnancy provided the basis for FMM following the detection of trophoblast cells in the lungs of deceased women. Read More

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http://dx.doi.org/10.3389/fimmu.2019.00659DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6455070PMC

Treatment algorithm for spontaneous spinal infections: A review of the literature.

J Craniovertebr Junction Spine 2019 Jan-Mar;10(1):3-9

Department of Human Neurosciences, Division of Neurosurgery, Sapienza University of Rome, Rome, Italy.

Background: Primary spinal infections are rare pathologies with an estimated incidence of 5% of all osteomyelitis. The diagnosis can be challenging and this might result in a late identification. The etiological diagnosis is the primary concern to determine the most appropriate treatment. Read More

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http://dx.doi.org/10.4103/jcvjs.JCVJS_115_18DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6469318PMC

Anti-Stress and Glial Differentiation Effects of a Novel Combination of Cucurbitacin B and Withanone (CucWi-N): Experimental Evidence.

Ann Neurosci 2018 Dec 5;25(4):201-209. Epub 2018 Jul 5.

DBT-AIST International laboratory for Advanced Biomedicine (DAILAB), National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Japan.

Background: Natural extracts and compounds used in traditional home medicine are known for their safety and a variety of health promoting and therapeutic potentials. In contrast to the single molecule mediated targets, the combinational therapies are preferred for their multi-functional and limited toxic regimens and may be useful for disease therapeutics as well as to increase the quality of life during a variety of environmental stresses.

Purpose: We aimed to combine the active ingredients of Chinese () and Indian () ginsengs to develop a natural, efficient, and welfare combinatorial mixture with high anti-stress and glial differentiation potentials. Read More

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https://www.karger.com/Article/FullText/490693
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http://dx.doi.org/10.1159/000490693DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6470339PMC
December 2018
1 Read

Isolation and characterisation of pVa-21, a giant bacteriophage with anti-biofilm potential against Vibrio alginolyticus.

Sci Rep 2019 Apr 18;9(1):6284. Epub 2019 Apr 18.

Laboratory of Aquatic Biomedicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul, Republic of Korea.

There is an increasing emergence of antibiotic-resistant Vibrio alginolyticus, a zoonotic pathogen that causes mass mortality in aquatic animals and infects humans; therefore, there is a demand for alternatives to antibiotics for the treatment and prevention of infections caused by this pathogen. One possibility is through the exploitation of bacteriophages. In the present study, the novel bacteriophage pVa-21 was classified as Myoviridae and characterised as a candidate biocontrol agent against V. Read More

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http://www.nature.com/articles/s41598-019-42681-1
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http://dx.doi.org/10.1038/s41598-019-42681-1DOI Listing
April 2019
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Common patterns of gene regulation associated with Cesarean section and the development of islet autoimmunity - indications of immune cell activation.

Sci Rep 2019 Apr 18;9(1):6250. Epub 2019 Apr 18.

Institute of Computational Biology, Helmholtz Zentrum München, Neuherberg, Germany.

Birth by Cesarean section increases the risk of developing type 1 diabetes later in life. We aimed to elucidate common regulatory processes observed after Cesarean section and the development of islet autoimmunity, which precedes type 1 diabetes, by investigating the transcriptome of blood cells in the developing immune system. To investigate Cesarean section effects, we analyzed longitudinal gene expression profiles from peripheral blood mononuclear cells taken at several time points from children with increased familial and genetic risk for type 1 diabetes. Read More

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http://dx.doi.org/10.1038/s41598-019-42750-5DOI Listing

Felodipine induces autophagy in mouse brains with pharmacokinetics amenable to repurposing.

Nat Commun 2019 Apr 18;10(1):1817. Epub 2019 Apr 18.

Department of Medical Genetics, Cambridge Institute for Medical Research, University of Cambridge, The Keith Peters Building, Cambridge Biomedical Campus, Hills Road, Cambridge, CB2 0XY, UK.

Neurodegenerative diseases like Alzheimer's disease, Parkinson's disease and Huntington's disease manifest with the neuronal accumulation of toxic proteins. Since autophagy upregulation enhances the clearance of such proteins and ameliorates their toxicities in animal models, we and others have sought to re-position/re-profile existing compounds used in humans to identify those that may induce autophagy in the brain. A key challenge with this approach is to assess if any hits identified can induce neuronal autophagy at concentrations that would be seen in humans taking the drug for its conventional indication. Read More

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http://www.nature.com/articles/s41467-019-09494-2
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http://dx.doi.org/10.1038/s41467-019-09494-2DOI Listing
April 2019
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L amino acid transporter structure and molecular bases for the asymmetry of substrate interaction.

Nat Commun 2019 Apr 18;10(1):1807. Epub 2019 Apr 18.

Institute for Research in Biomedicine (IRB Barcelona), Barcelona Institute of Science and Technology, 08028, Barcelona, Spain.

L-amino acid transporters (LATs) play key roles in human physiology and are implicated in several human pathologies. LATs are asymmetric amino acid exchangers where the low apparent affinity cytoplasmic side controls the exchange of substrates with high apparent affinity on the extracellular side. Here, we report the crystal structures of an LAT, the bacterial alanine-serine-cysteine exchanger (BasC), in a non-occluded inward-facing conformation in both apo and substrate-bound states. Read More

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http://dx.doi.org/10.1038/s41467-019-09837-zDOI Listing

Dishevelled-3 conformation dynamics analyzed by FRET-based biosensors reveals a key role of casein kinase 1.

Nat Commun 2019 Apr 18;10(1):1804. Epub 2019 Apr 18.

Department of Experimental Biology, Faculty of Science, Masaryk University, Brno, 62500, Czech Republic.

Dishevelled (DVL) is the key component of the Wnt signaling pathway. Currently, DVL conformational dynamics under native conditions is unknown. To overcome this limitation, we develop the Fluorescein Arsenical Hairpin Binder- (FlAsH-) based FRET in vivo approach to study DVL conformation in living cells. Read More

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http://dx.doi.org/10.1038/s41467-019-09651-7DOI Listing

Amygdala ensembles encode behavioral states.

Science 2019 Apr 18;364(6437). Epub 2019 Apr 18.

Friedrich Miescher Institute for Biomedical Research, Maulbeerstrasse 66, Basel, Switzerland.

Internal states, including affective or homeostatic states, are important behavioral motivators. The amygdala regulates motivated behaviors, yet how distinct states are represented in amygdala circuits is unknown. By longitudinally imaging neural calcium dynamics in freely moving mice across different environments, we identified opponent changes in activity levels of two major, nonoverlapping populations of basal amygdala principal neurons. Read More

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http://dx.doi.org/10.1126/science.aav8736DOI Listing

Enhanced dendritic inhibition and impaired NMDAR activation in a mouse model of Down syndrome.

J Neurosci 2019 Apr 18. Epub 2019 Apr 18.

Department of Biomedicine, University of Basel, Pestalozzistr. 20, CH-4056 Basel, Switzerland

Down syndrome (DS) or Trisomy 21 is a developmental disorder leading to cognitive deficits including disruption of hippocampus-dependent learning and memory. Enhanced inhibition has been suggested to underlie these deficits in DS based on studies using the Ts65Dn mouse model. Here we show that in this mouse model, GABAergic synaptic inhibition onto dendrites of hippocampal pyramidal cells is increased. Read More

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http://www.jneurosci.org/lookup/doi/10.1523/JNEUROSCI.2723-1
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http://dx.doi.org/10.1523/JNEUROSCI.2723-18.2019DOI Listing
April 2019
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Complete Genome Sequence of a Bioactive Pseudomonas sp. Strain, DTU12.3, Isolated from Soil in Denmark.

Microbiol Resour Announc 2019 Apr 18;8(16). Epub 2019 Apr 18.

Department of Biotechnology and Biomedicine, Technical University of Denmark, Kongens Lyngby, Denmark

Here, we report the complete annotated genome sequence of a sp. strain, DTU12.3. Read More

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http://dx.doi.org/10.1128/MRA.00121-19DOI Listing

Mouse ANKRD31 Regulates Spatiotemporal Patterning of Meiotic Recombination Initiation and Ensures Recombination between X and Y Sex Chromosomes.

Mol Cell 2019 Apr 12. Epub 2019 Apr 12.

Institute of Physiological Chemistry, Faculty of Medicine, Technische Universität Dresden, Fetscherstraße 74, 01307 Dresden, Germany. Electronic address:

Orderly segregation of chromosomes during meiosis requires that crossovers form between homologous chromosomes by recombination. Programmed DNA double-strand breaks (DSBs) initiate meiotic recombination. We identify ANKRD31 as a key component of complexes of DSB-promoting proteins that assemble on meiotic chromosome axes. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S10972765193022
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http://dx.doi.org/10.1016/j.molcel.2019.03.022DOI Listing
April 2019
4 Reads

New insights into the binding mechanism between osthole and β-lactoglobulin: Spectroscopic, chemometrics and docking studies.

Food Res Int 2019 Jun 22;120:226-234. Epub 2019 Feb 22.

State Key Laboratory of Food Science and Technology, Nanchang University, 235 Nanjing East Road, Nanchang 330047, China. Electronic address:

Osthole, a type of coumarin derivative, owns many biological functions, but the poor water solubility and low bioavailability limit its usage in food and pharmaceutical fields. β-Lactoglobulin (BLG) is a major protein in bovine milk whey which is usually used as a carrier of poorly soluble bioactive components. Researching the interaction between osthole and BLG can help us in understanding their binding mechanism and design an osthole delivery system using BLG as carrier protein to further promote the application of osthole in functional food and drug. Read More

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http://dx.doi.org/10.1016/j.foodres.2019.02.042DOI Listing

T2, T2 and spin coupling ratio as biomarkers for the study of lipomatous tumors.

Phys Med 2019 Apr 28;60:76-82. Epub 2019 Mar 28.

Department of Radiology, University of Crete, Heraklion, Crete, Greece.

Background: Subcutaneous fat may have variable signal intensity on T2w images depending on the choice of imaging parameters. However, fatty components within tumors have a different degree of signal dependence on the acquisition scheme. This study examined the use of T2, T2 relaxometry and spin coupling related signal changes (Spin Coupling ratio, SCr) on two different imaging protocols as clinically relevant descriptors of benign and malignant lipomatous tumors. Read More

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http://dx.doi.org/10.1016/j.ejmp.2019.03.023DOI Listing

Impact of polyols on Oral microbiome of Estonian schoolchildren.

BMC Oral Health 2019 Apr 18;19(1):60. Epub 2019 Apr 18.

Department of Microbiology, Institute of Biomedicine and Translational Medicine, University of Tartu, Tartu, Estonia.

Background: Oral microbiome has significant impact on both oral and general health. Polyols have been promoted as sugar substitutes in prevention of oral diseases. We aimed to reveal the effect of candies containing erythritol, xylitol or control (sorbitol) on salivary microbiome. Read More

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http://dx.doi.org/10.1186/s12903-019-0747-zDOI Listing

Metabolic and signalling network maps integration: application to cross-talk studies and omics data analysis in cancer.

BMC Bioinformatics 2019 Apr 18;20(Suppl 4):140. Epub 2019 Apr 18.

Institut Curie, 26 rue d'Ulm, F-75005, Paris, France.

Background: The interplay between metabolic processes and signalling pathways remains poorly understood. Global, detailed and comprehensive reconstructions of human metabolism and signalling pathways exist in the form of molecular maps, but they have never been integrated together. We aim at filling in this gap by integrating of both signalling and metabolic pathways allowing a visual exploration of multi-level omics data and study of cross-regulatory circuits between these processes in health and in disease. Read More

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http://dx.doi.org/10.1186/s12859-019-2682-zDOI Listing

Dose-Response Meta-analysis: Application and Practice using R software.

Epidemiol Health 2019 Mar 28:e2019006. Epub 2019 Mar 28.

Department of Internal Medicine, Jeju National University Hospital, Jeju National University School of Medicine, Jeju, Korea.

The objective of this study was to describe general approaches of dose-response meta-analysis that are available for quantitative synthesis of data using R software. We conducted a dose-response meta-analysis using two types of data that included difference in means in continuous data and odds ratio in binary data. The package commands of R software were doseresmeta for overall effect sizes that were separated linear model, quadratic model, and restricted cubic split model in order to well understanding. Read More

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http://dx.doi.org/10.4178/epih.e2019006DOI Listing

Diagnostic Test Accuracy: Application and Practice using R software.

Epidemiol Health 2019 Mar 28:e2019007. Epub 2019 Mar 28.

Department of Internal Medicine, Jeju National University Hospital, Jeju National University School of Medicine, Jeju, Korea.

The objective of this study was to describe general approaches of diagnostic test accuracy that are available for quantitative synthesis of data using R software. We conducted a diagnostic test accuracy that summarized statistics for univariate analysis and bivariate analysis. The package commands of R software were metaprop and metabin for sensitivity, specificity, and diagnostic odds ratio, forest for forest plot, reitsma of mada for summarized ROC curve, and metareg for meta-regression analysis. Read More

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http://dx.doi.org/10.4178/epih.e2019007DOI Listing

Intervention Meta-analysis: Application and Practice using R software.

Epidemiol Health 2019 Mar 28:e2019008. Epub 2019 Mar 28.

Department of Nuclear Medicine, College of Medicine, Pusan National University, Yangsan, Korea.

The objective of this study was to describe general approaches of intervention meta-analysis that are available for quantitative synthesis of data using R software. We conducted an intervention meta-analysis using two types of data that included difference in means in continuous data and odds ratio in binary data. The package commands of R software were metacont, metabin, and metagen for overall effect size, forest for forest plot, metareg for meta-regression analysis, and funnel and metabias for publication bias. Read More

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http://dx.doi.org/10.4178/epih.e2019008DOI Listing

Network Meta-analysis: Application and Practice using R software.

Epidemiol Health 2019 Apr 8:e2019013. Epub 2019 Apr 8.

Institute of Medical Biometry and Statistics, Faculty of Medicine and Medical Center - University of Freiburg, Freiburg, Germany.

The objective of this study was to describe general approaches of network meta-analysis that are available for quantitative synthesis of data using R software. We conducted a network meta-analysis using two types of concepts: Bayesian and frequentist approach. The packages of R software were gemtc for Bayesian approach and netmeta for frequentist approach. Read More

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http://e-epih.org/journal/view.php?doi=10.4178/epih.e2019013
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http://dx.doi.org/10.4178/epih.e2019013DOI Listing
April 2019
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Advances in the Understanding of Skin Cancer: Ultraviolet Radiation, Mutations, and Antisense Oligonucleotides as Anticancer Drugs.

Molecules 2019 Apr 17;24(8). Epub 2019 Apr 17.

Medical Academy named after S.I. Georgievsky, V.I. Vernadsky Crimean Federal University, Lenin Boulevard 5/7, 295051 Simferopol, Crimea.

Skin cancer has always been and remains the leader among all tumors in terms of occurrence. One of the main factors responsible for skin cancer, natural and artificial UV radiation, causes the mutations that transform healthy cells into cancer cells. These mutations inactivate apoptosis, an event required to avoid the malignant transformation of healthy cells. Read More

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http://dx.doi.org/10.3390/molecules24081516DOI Listing

Effects of Neck Taping in the Treatment of Hemispatial Neglect in Chronic Stroke Patients: A Pilot, Single Blind, Randomized Controlled Trial.

Medicina (Kaunas) 2019 Apr 17;55(4). Epub 2019 Apr 17.

Neuromotor and Cognitive Rehabilitation Research Center, Department of Neurosciences, Biomedicine and Movement Sciences, University of Verona, 37134 Verona, Italy.

Hemispatial neglect is a common consequence of stroke, with an estimated incidence of 23%. Interventions for treating hemispatial neglect may be categorized as either top-down or bottom-up processing. The aim of top-down approaches is to train the person to voluntarily compensate for their neglect. Read More

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https://www.mdpi.com/1010-660X/55/4/108
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http://dx.doi.org/10.3390/medicina55040108DOI Listing
April 2019
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The PTEN⁻PI3K Axis in Cancer.

Biomolecules 2019 Apr 17;9(4). Epub 2019 Apr 17.

Cancer Research Institute, Beth Israel Deaconess Cancer Center, Department of Medicine and Pathology, Beth Israel Deaconess Medical Center (BIDMC), Harvard Medical School, Boston, MA 02215, USA.

The PI3K-AKT-mTOR signal transduction pathway regulates a variety of biological processes including cell growth, cell cycle progression and proliferation, cellular metabolism, and cytoskeleton reorganization. Fine-tuning of the phosphatidylinositol 3-kinase (PI3K) pathway signaling output is essential for the maintenance of tissue homeostasis and uncontrolled activation of this cascade leads to a number of human pathologies including cancer. Inactivation of the tumor suppressor phosphatase and tensin homologue deleted on Chromosome 10 (PTEN) and/or activating mutations in the proto-typical lipid kinase PI3K have emerged as some of the most frequent events associated with human cancer and as a result the PI3K pathway has become a highly sought-after target for cancer therapies. Read More

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https://www.mdpi.com/2218-273X/9/4/153
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http://dx.doi.org/10.3390/biom9040153DOI Listing
April 2019
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Vasculogenic Chronic Ulcer: Tissue Regeneration with an Innovative Dermal Substitute.

J Clin Med 2019 Apr 17;8(4). Epub 2019 Apr 17.

Department of Surgical Science, Plastic and Reconstructive Surgery Unit, University of Rome Tor Vergata, 00133 Rome, Italy.

The healing of venous and arterial ulcers is slow, and in some cases, they may not heal at all. This study aims to demonstrate the clinical advantage of Nevelia, an innovative collagen dermal template substitute (DS) in venous and arterial chronic ulcers treatment. 35 patients affected by chronic vascular ulcers with a mean area of 35. Read More

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https://www.mdpi.com/2077-0383/8/4/525
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http://dx.doi.org/10.3390/jcm8040525DOI Listing
April 2019
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Screening for mutations in selected miRNA genes in hypogonadotropic hypogonadism patients.

Endocr Connect 2019 May;8(5):506-509

Institute of Biomedicine/Physiology, Biomedicum Helsinki and Stem Cells and Metabolism Research Program, University of Helsinki, Helsinki, Finland.

In approximately half of congenital hypogonadotropic hypogonadism (cHH) patients, the genetic cause remains unidentified. Since the lack of certain miRNAs in animal models has led to cHH, we sequenced human miRNAs predicted to regulate cHH-related genes (MIR7-3, MIR141, MIR429 and MIR200A-C) in 24 cHH patients with Sanger sequencing. A heterozygous variant in MIR200A (rs202051309; general population frequency of 0. Read More

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https://ec.bioscientifica.com/view/journals/ec/8/5/EC-19-008
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http://dx.doi.org/10.1530/EC-19-0080DOI Listing
May 2019
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On the critical evaluation and confirmation of germline sequence variants identified using massively parallel sequencing.

J Biotechnol 2019 Apr 15. Epub 2019 Apr 15.

Institute for Clinical and Translational Research, Biomedical Research Center, Slovak Academy of Sciences, Bratislava, Slovakia; Geneton Ltd., Bratislava, Slovakia. Electronic address:

Although massively parallel sequencing (MPS) is becoming common practice in both research and routine clinical care, confirmation requirements of identified DNA variants using alternative methods are still topics of debate. When evaluating variants directly from MPS data, different read depth statistics, together with specialized genotype quality scores are, therefore, of high relevance. Here we report results of our validation study performed in two different ways: 1) confirmation of MPS identified variants using Sanger sequencing; and 2) simultaneous Sanger and MPS analysis of exons of selected genes. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01681656193012
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http://dx.doi.org/10.1016/j.jbiotec.2019.04.013DOI Listing
April 2019
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Native aortic versus mitral valve infective endocarditis: a nationwide registry study.

Open Heart 2019 27;6(1):e000926. Epub 2019 Feb 27.

Division of Cardiothoracic Surgery, Department for Clinical Sciences Lund, Skane University Hospital and Lund University, Lund, Sweden.

Background: Native aortic and mitral valve infective endocarditis (AVE and MVE, respectively) are usually grouped together as left-sided native valve infective endocarditis (LNVE), while the differences between AVE and MVE have not yet been properly investigated. We aimed to compare AVE and MVE in regard to patient characteristics, microbiology and determinants of survival.

Methods: We conducted a retrospective study using the Swedish national registry on infective endocarditis, which contains nationwide patient data. Read More

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http://openheart.bmj.com/lookup/doi/10.1136/openhrt-2018-000
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http://dx.doi.org/10.1136/openhrt-2018-000926DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6443117PMC
February 2019
1 Read

PM induced neurodegenerative-like changes in mice and the antagonistic effects of vitamin E.

Toxicol Res (Camb) 2019 Mar 8;8(2):172-179. Epub 2019 Jan 8.

Department of Food science and Engineering , Moutai Institute , Renhuai , 564507 , China.

Neurodegenerative diseases (ND) affect a large and ever-growing population globally, resulting in heavy burdens for patients and their families. Though some specific genes related to ND have been identified, the genetic factors fail to fully account for the pathogenesis of ND. Therefore, the roles of the environment and life styles in the occurrence of ND are being actively probed. Read More

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http://xlink.rsc.org/?DOI=C8TX00333E
Publisher Site
http://dx.doi.org/10.1039/c8tx00333eDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6425994PMC
March 2019
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Functional black phosphorus nanosheets for mitochondria-targeting photothermal/photodynamic synergistic cancer therapy.

Chem Sci 2019 Apr 21;10(13):3779-3785. Epub 2019 Feb 21.

Key Laboratory of Flexible Electronics (KLOFE) , Institute of Advanced Materials (IAM) , School of Physical and Mathematical Science , Nanjing Tech University (NanjingTech) , 30 South Puzhu Road , Nanjing 211800 , China . Email: ; Email: ; Email:

Organelle-targeting nanosystems are envisioned as promising tools for phototherapy, which can generate heat or reactive oxygen species (ROS) induced cytotoxicity in the targeted location but leave the surrounding biological tissues undamaged. In this work, an imaging-guided mitochondria-targeting photothermal/photodynamic nanosystem has been developed on the basis of functionalized black phosphorus (BP) nanosheets (NSs). In the nanosystem, BP NSs are coated with polydopamine (PDA) and then covalently linked with both chlorin e6 (Ce6) and triphenyl phosphonium (TPP) through carbodiimide reaction between the amino group and the carboxyl group, forming BP@PDA-Ce6&TPP NSs. Read More

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http://dx.doi.org/10.1039/c8sc04844dDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6446962PMC

Spectrum of mutations in the gene in Vietnamese patients with retinoblastoma.

Mol Vis 2019 4;25:215-221. Epub 2019 Apr 4.

Center for Molecular Biomedicine, University of Medicine and Pharmacy at Ho Chi Minh City, Ho Chi Minh City, Vietnam.

Purpose: Retinoblastoma (RB) is a rare childhood malignant disorder caused by the biallelic inactivation of the gene. Early diagnosis and identification of carriers of heritable mutations in can improve disease outcome and management. In this study, we present the spectrum of mutations in the gene in Vietnamese patients with RB. Read More

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April 2019
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[Chronic phosphoproteomic in temporal lobe epilepsy mouse models induced by kainic acid].

Beijing Da Xue Xue Bao Yi Xue Ban 2019 Apr;51(2):197-205

Institute of Systems Biomedicine, State Key Laboratory of Natural and Biomimetic Drugs, Department of Molecular and Cellular Pharmacology, Peking University School of Pharmaceutical Science, Beijing 100191, China.

Objective: To investigate functions of proteins and signaling pathways involved in epileptogenesis during the chronic stage of temporal lobe epilepsy in mouse models.

Methods: Kainic acid-induced temporal lobe epilepsy models were conducted, when reaching stage 4 using racine scale, the mice of experimental group were supposed to be successfully established. Pentobarbital sodium was injected to stop epileptic seizure in case of death. Read More

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April 2019
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The GDPR and the research exemption: considerations on the necessary safeguards for research biobanks.

Eur J Hum Genet 2019 Apr 17. Epub 2019 Apr 17.

Centre for Biomedicine, EURAC, Bolzano and CRB Uppsala University Sweden, Uppsala, Sweden.

The General Data Protection Regulation (GDPR) came into force in May 2018. The aspiration of providing for a high level of protection to individuals' personal data risked placing considerable constraints on scientific research, which was contrary to various research traditions across the EU. Therefore, along with the set of carefully outlined data subjects' rights, the GDPR provides for a two-level framework to enable derogations from these rights when scientific research is concerned. Read More

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http://dx.doi.org/10.1038/s41431-019-0386-5DOI Listing

LRSAM1 variants and founder effect in French families with ataxic form of Charcot-Marie-Tooth type 2.

Eur J Hum Genet 2019 Apr 17. Epub 2019 Apr 17.

AP-HP, G-H Pitié-Salpêtrière, Centre de Référence des Maladies neuromusculaires, Paris Nord/Est/Ile de france, Paris, France.

Currently only 25-30% of patients with axonal forms of Charcot-Marie-Tooth disease (CMT) receive a genetic diagnosis. We aimed to identify the causative gene of CMT type 2 in 8 non-related French families with a distinct clinical phenotype. We collected clinical, electrophysiological, and laboratory findings and performed genetic analyses in four different French laboratories. Read More

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http://dx.doi.org/10.1038/s41431-019-0403-8DOI Listing

High-throughput screening discovers antifibrotic properties of haloperidol by hindering myofibroblast activation.

JCI Insight 2019 Apr 18;4(8). Epub 2019 Apr 18.

Cardiovascular Biology.

Fibrosis is a hallmark in the pathogenesis of various diseases, with very limited therapeutic solutions. A key event in the fibrotic process is the expression of contractile proteins, including α-smooth muscle actin (αSMA) by fibroblasts, which become myofibroblasts. Here, we report the results of a high-throughput screening of a library of approved drugs that led to the discovery of haloperidol, a common antipsychotic drug, as a potent inhibitor of myofibroblast activation. Read More

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http://dx.doi.org/10.1172/jci.insight.123987DOI Listing

A Live Vaccine Delivering PcrV through the Type III Secretion System Protects against Pseudomonas aeruginosa.

mSphere 2019 Apr 17;4(2). Epub 2019 Apr 17.

Departamento de Genética, Facultad de Biología, Universidad de Sevilla, Seville, Spain

is a common Gram-negative opportunistic pathogen that is intrinsically resistant to a wide range of antibiotics. The development of a broadly protective vaccine against remains a major challenge. Here, we used an attenuated strain of serovar Typhimurium as a vehicle to express antigens. Read More

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http://dx.doi.org/10.1128/mSphere.00116-19DOI Listing

Brody disease: when myotonia is not myotonia.

Pract Neurol 2019 Apr 17. Epub 2019 Apr 17.

Neurology Department, Centro Hospitalar Universitário de São João, Porto, Portugal.

A 56-year-old man presented with painless impairment of muscle relaxation on vigorous contraction (eg, eyelid closure, hand grip, running). There were no episodes of paralysis, symptom progression, weakness or extramuscular symptoms. Five of his fifteen siblings had similar complaints. Read More

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http://pn.bmj.com/lookup/doi/10.1136/practneurol-2019-002224
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http://dx.doi.org/10.1136/practneurol-2019-002224DOI Listing
April 2019
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Relationship between changes in resting-state spontaneous brain activity and cognitive impairment in patients with CADASIL.

J Headache Pain 2019 Apr 17;20(1):36. Epub 2019 Apr 17.

Department of Neurology and Jiuyuan Municipal Stroke Center, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, 639 Zhizaoju Road, Shanghai, 200011, People's Republic of China.

Background: Cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL) mainly manifests with cognitive impairment. Cognitive deficits in patients with CADASIL are correlated with structural brain changes such as lacunar lesion burden, normalized brain volume, and anterior thalamic radiation lesions, but changes in resting-state functional brain activity in patients with CADASIL have not been reported.

Methods: This study used resting-state functional magnetic resonance imaging (fMRI) to measure the amplitude of low-frequency fluctuation (ALFF) in 22 patients with CADASIL and 44 healthy matched controls. Read More

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http://dx.doi.org/10.1186/s10194-019-0982-3DOI Listing

Rare case of an oligospermic male with 46,XX/46,XY tetragametic chimerism.

Andrologia 2019 Apr 17:e13290. Epub 2019 Apr 17.

Department of Genetics, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran.

Chimerism, a rare human disorder, is assumed to be the result of an amalgamation of two separate zygotes in a single embryo. Studies have shown that the phenotypic spectrum of chimerism is variable and there is no definite genotype-phenotype correlation in patients with chimerism, therefore a majority of cases might remain undiagnosed. This study aims to investigate the possible mechanism of the chimerism in a 46,XX/46,XY infertile and phenotypically normal male, with 46,XX blood karyotype and normal spermatogenesis. Read More

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http://dx.doi.org/10.1111/and.13290DOI Listing

Elastic properties and intrinsic strength of two-dimensional InSe flakes.

Nanotechnology 2019 Apr 17. Epub 2019 Apr 17.

University of Washington, Seattle, Washington, UNITED STATES.

Mechanical property of two-dimensional (2D) materials is critical for their applications in functional devices as well as for strain engineering. Here, we report the Young's modulus and breaking strength of multilayered InSe, an emerging 2D semiconductors of layered group III chalcogenide. Few-layer InSe flaks were exfoliated from bulk InSe crystal onto Si/SiO2 substrate with micro-fabricated holes, and indentation tests were carried using an Atomic Force Microscopy (AFM) probe. Read More

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http://iopscience.iop.org/article/10.1088/1361-6528/ab1a96
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http://dx.doi.org/10.1088/1361-6528/ab1a96DOI Listing
April 2019
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Transforming Growth Factor-β Signaling in Immunity and Cancer.

Immunity 2019 Apr;50(4):924-940

Cancer Biology and Genetics Program, Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA. Electronic address:

Transforming growth factor (TGF)-β is a crucial enforcer of immune homeostasis and tolerance, inhibiting the expansion and function of many components of the immune system. Perturbations in TGF-β signaling underlie inflammatory diseases and promote tumor emergence. TGF-β is also central to immune suppression within the tumor microenvironment, and recent studies have revealed roles in tumor immune evasion and poor responses to cancer immunotherapy. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S10747613193014
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http://dx.doi.org/10.1016/j.immuni.2019.03.024DOI Listing
April 2019
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