57,530 results match your criteria Biomedicine & Pharmacotherapy[Journal]


Fetal -derived macrophages persist and sequentially maturate in ovaries after birth in mice.

Eur J Immunol 2020 May 27. Epub 2020 May 27.

Institute of Biomedicine, University of Turku, Turku, FI-20520, Finland.

Macrophages, which are highly diverse in different tissues, play a complex and vital role in tissue development, homeostasis and inflammation. The origin and heterogeneity of tissue-resident monocytes and macrophages in ovaries remains unknown. Here we identify three tissue-resident monocyte populations and five macrophage populations in the adult ovaries using high-dimensional single cell mass cytometry. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/eji.202048531DOI Listing

Increased therapeutic effect on medullary thyroid cancer using a combination of radiation and tyrosine kinase inhibitors.

PLoS One 2020 27;15(5):e0233720. Epub 2020 May 27.

Department of Radiation Physics, Institute of Clinical Sciences, Sahlgrenska Cancer Center, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.

Since patients with medullary thyroid cancer (MTC) often have metastatic disease at the time of diagnosis, the development of efficient systemic treatment options for MTC is important. Vandetanib and cabozantinib are two tyrosine kinase inhibitors (TKIs) that were recently approved by FDA and EMA for systemic treatment of metastatic MTC. Additionally, since MTC is of a neuroendocrine tumour type, treatment with radiolabelled somatostatin analogues (e. Read More

View Article

Download full-text PDF

Source
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0233720PLOS

Comparison of self- and nurse-measured office blood pressure in patients with chronic kidney disease.

Blood Press Monit 2020 May 25. Epub 2020 May 25.

Department of Renal Medicine, Aarhus University Hospital.

Objective: As blood pressure (BP) control is very important in chronic kidney disease (CKD), we investigated how office BP is influenced by the measurement circumstances and compared nonautomated self- and nurse-measured BP values.

Materials And Methods: Two hundred stage 1-5 CKD patients with scheduled visits to an outpatient clinic were randomized to either self-measured office BP (SMOBP) followed by nurse-measured office BP (NMOBP) or NMOBP followed by SMOBP. The participants had been educated to perform the self-measurement in at least one previous visit. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1097/MBP.0000000000000453DOI Listing

EpiRegio: analysis and retrieval of regulatory elements linked to genes.

Nucleic Acids Res 2020 May 27. Epub 2020 May 27.

Institute for Cardiovascular Regeneration, Goethe University Hospital, 60590 Frankfurt am Main, Germany.

A current challenge in genomics is to interpret non-coding regions and their role in transcriptional regulation of possibly distant target genes. Genome-wide association studies show that a large part of genomic variants are found in those non-coding regions, but their mechanisms of gene regulation are often unknown. An additional challenge is to reliably identify the target genes of the regulatory regions, which is an essential step in understanding their impact on gene expression. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1093/nar/gkaa382DOI Listing

PaCRISPR: a server for predicting and visualizing anti-CRISPR proteins.

Nucleic Acids Res 2020 May 27. Epub 2020 May 27.

Infection and Immunity Program, Biomedicine Discovery Institute and Department of Microbiology, Monash University, VIC 3800, Australia.

Anti-CRISPRs are widespread amongst bacteriophage and promote bacteriophage infection by inactivating the bacterial host's CRISPR-Cas defence system. Identifying and characterizing anti-CRISPR proteins opens an avenue to explore and control CRISPR-Cas machineries for the development of new CRISPR-Cas based biotechnological and therapeutic tools. Past studies have identified anti-CRISPRs in several model phage genomes, but a challenge exists to comprehensively screen for anti-CRISPRs accurately and efficiently from genome and metagenome sequence data. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1093/nar/gkaa432DOI Listing

Molecular, serological, and biochemical diagnosis and monitoring of COVID-19: IFCC taskforce evaluation of the latest evidence.

Clin Chem Lab Med 2020 May 27. Epub 2020 May 27.

IFCC Taskforce on COVID-19, International Federation of Clinical Chemistry, Milan, Italy.

The global coronavirus disease 2019 (COVID-19) has presented major challenges for clinical laboratories, from initial diagnosis to patient monitoring and treatment. Initial response to this pandemic involved the development, production, and distribution of diagnostic molecular assays at an unprecedented rate, leading to minimal validation requirements and concerns regarding their diagnostic accuracy in clinical settings. In addition to molecular testing, serological assays to detect antibodies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are now becoming available from numerous diagnostic manufacturers. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1515/cclm-2020-0722DOI Listing

Assessing risk of bias judgments for blinding of outcome assessors in Cochrane reviews.

J Comp Eff Res 2020 May 27. Epub 2020 May 27.

Center for Evidence-Based Medicine & Health Care, Catholic University of Croatia, Zagreb, Croatia.

Adequate judging of risk of bias (RoB) for blinding of outcome assessors (detection bias) is important for supporting highest level of evidence. Judgments and supporting comments for detection bias were retrieved from RoB tables reported in Cochrane reviews. We categorized comments, and then compared judgment and supporting comment with instructions from the Cochrane Handbook. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.2217/cer-2019-0181DOI Listing

Seeing the Confetti Colors in a New Light Utilizing Flow Cytometry and Imaging Flow Cytometry.

Cytometry A 2020 May 27. Epub 2020 May 27.

Department of Biomedicine, Aarhus University, Aarhus C, Denmark.

Stochastic multicolor transgenic labeling systems, such as the Brainbow reporters, have emerged as powerful tools in lineage tracing experiments. Originally designed for large-scale mapping of neuronal projections in densely populated tissues, they have been repurposed for diverse uses. The Brainbow 2. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/cyto.a.24032DOI Listing

Factors Motivating Traditional Healer versus Biomedical Facility Use for Treatment of Pediatric Febrile Illness: Results from a Qualitative Study in Southwestern Uganda.

Am J Trop Med Hyg 2020 May 26. Epub 2020 May 26.

Center for Global Health, Weill Cornell Medicine, New York, New York.

Febrile illnesses, such as malaria and pneumonia, are among the most common causes of mortality in children younger than 5 years in Uganda outside of the neonatal period. Their impact could be mitigated through earlier diagnosis and treatment at biomedical facilities; however, it is estimated that a large percentage of Ugandans (70-80%) seek traditional healers for their first line of medical care. This study sought to characterize individual and structural influences on health care-seeking behaviors for febrile children. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.4269/ajtmh.19-0897DOI Listing

Construction and Characterization of a Novel Recombinant Attenuated and Replication-Deficient Candidate Human Adenovirus Type 3 Vaccine: "Adenovirus Vaccine Within an Adenovirus Vector".

Virol Sin 2020 May 26. Epub 2020 May 26.

Guangdong Provincial Key Laboratory of Tropical Disease Research, School of Public Health, Southern Medical University, Guangzhou, 510515, China.

Human adenoviruses (HAdVs) are highly contagious and result in large number of acute respiratory disease (ARD) cases with severe morbidity and mortality. Human adenovirus type 3 (HAdV-3) is the most common type that causes ARD outbreaks in Asia, Europe, and the Americas. However, there is currently no vaccine approved for its general use. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s12250-020-00234-1DOI Listing

The synergy between LPMOs and cellulases in enzymatic saccharification of cellulose is both enzyme- and substrate-dependent.

Biotechnol Lett 2020 May 26. Epub 2020 May 26.

Department of Plant and Environmental Sciences, University of Copenhagen, 1871, Frederiksberg, Denmark.

Objectives: The synergistic effects between cellulases and lytic polysaccharide monooxygenases (LPMOs) were investigated systematically in terms of their degree of synergy (DS) on amorphous and crystalline cellulose. Synergy curves were obtained for enzyme pairs containing a cellulase from Trichoderma reesei (Cel6A and Cel7A) and three LPMOs from Thermoascus aurantiacus (TaAA9A), Lentinus similis (LsAA9A) and Thielavia terrestris (TtAA9E).

Results: The synergistic experiments showed that the three LPMOs significantly improved the hydrolytic efficiency of Cel6A, on both cellulosic substrates; a more pronounced effect being seen for TtAA9E on amorphous cellulose at low cellulase:LPMO ratios. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s10529-020-02922-0DOI Listing

A parsimonious score with a free web tool for predicting disability after an ischemic stroke: the Parsifal Score.

J Neurol 2020 May 26. Epub 2020 May 26.

Neurology Service, IMIM-Hospital del Mar, Barcelona, Spain.

Background: Most of the models to predict prognosis after an ischemic stroke include complex mathematical equations or too many variables, making them difficult to use in the daily clinic. We want to predict disability 3 months after an ischemic stroke in an independent patient not receiving recanalization treatment within the first 24 h, using a minimum set of variables and an easy tool to facilitate its implementation. As a secondary aim, we calculated the capacity of the score to predict an excellent/devastating outcome and mortality. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00415-020-09914-0DOI Listing

In silico identification of new inhibitors for βeta-2-glycoprotein I as a major antigen in antiphospholipid antibody syndrome.

J Mol Model 2020 May 26;26(6):156. Epub 2020 May 26.

Reproductive Epidemiology Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran.

Beta 2 glycoprotein I (β2GPI) is a major antigen for autoantibodies present in antiphospholipid antibody syndrome (APS). β2GPI is a single polypeptide with five repeated domains and different conformations. The activated J-shaped conformation of β2GPI binds to negatively charged phospholipids in the membrane via the fifth domain and causes blood clotting reactions. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00894-020-04406-4DOI Listing

Sensory profiles in women with neuropathic pain after breast cancer surgery.

Breast Cancer Res Treat 2020 May 27. Epub 2020 May 27.

Division of Pain Medicine, Department of Anesthesiology, Intensive Care and Pain Medicine, Pain Clinic, University of Helsinki and Helsinki University Hospital, P.O. Box 140, 00029 HUS, Helsinki, Finland.

Purpose: We performed a detailed analysis of sensory function in patients with chronic post-surgical neuropathic pain (NP) after breast cancer treatments by quantitative sensory testing (QST) with DFNS (German Research Network on Neuropathic Pain) protocol and bed side examination (BE). The nature of sensory changes in peripheral NP may reflect distinct pathophysiological backgrounds that can guide the treatment choices. NP with sensory gain (i. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s10549-020-05681-8DOI Listing

IUI and uterine lavage of in vivo-produced blastocysts for PGT purposes: is it a technically and ethically reasonable perspective? Is it actually needed?

J Assist Reprod Genet 2020 May 26. Epub 2020 May 26.

Department of Biomedicine and Prevention, Section of Histology and Embryology, University of Rome "Tor Vergata", Rome, Italy.

A recent study by Munné et al. portrayed a protocol to retrieve in vivo produced blastocysts after IUI and uterine lavage for preimplantation genetic testing (PGT) purposes. The authors claimed this protocol might represent a reasonable future perspective for patients who do not want to undergo IVF, but still want to be informed about their embryos' genetic/chromosomal defects. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s10815-020-01813-7DOI Listing

Alginate Formulations: Current Developments in the Race for Hydrogel-Based Cardiac Regeneration.

Front Bioeng Biotechnol 2020 8;8:414. Epub 2020 May 8.

Department of Medicine and Surgery, University of Parma, Parma, Italy.

Cardiovascular diseases, including myocardial infarction (MI), represent the main worldwide cause of mortality and morbidity. In this scenario, to contrast the irreversible damages following MI, cardiac regeneration has emerged as a novel and promising solution for cellular regeneration, preserving cell behavior and tissue cytoarchitecture. Among the huge variety of natural, synthetic, and hybrid compounds used for tissue regeneration, alginate emerged as a good candidate for cellular preservation and delivery, becoming one of the first biomaterial tested in pre-clinical research and clinical trials concerning cardiovascular diseases. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3389/fbioe.2020.00414DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7226066PMC

Platelet-Membrane-Camouflaged Zirconia Nanoparticles Inhibit the Invasion and Metastasis of Hela Cells.

Front Chem 2020 7;8:377. Epub 2020 May 7.

Clinical Laboratory of the Third Xiangya Hospital, Central South University, Changsha, China.

Zirconia nanoparticles (ZrO2 NPs) are widely applied in the field of biomedicine. In this study, we constructed a nanoplatform of ZrO2 NPs coated with a platelet membrane (PLTm), named PLT@ZrO2. PLTm nanovesicles camouflage ZrO2 NPs, prevent nanoparticles from being cleared by macrophage, and target tumor sites. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3389/fchem.2020.00377DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7221201PMC

Public Health's Next Step in Advancing Equity: Re-evaluating Epistemological Assumptions to Move Social Determinants From Theory to Practice.

Front Public Health 2020 7;8:131. Epub 2020 May 7.

Department of Health Promotion and Behavioral Sciences, School of Public Health and Information Sciences, University of Louisville, Louisville, KY, United States.

The field of public health has increasingly promoted a social ecological approach to health, shifting from an individual, biomedical paradigm to a recognition of social and structural determinants of health and health equity. Yet despite this shift, public health research and practice continue to privilege individual- and interpersonal-level measurements and interventions. Rather than adapting public health practice to social ecological theory, the field has layered new concepts ("root causes," "social determinants") onto a biomedical paradigm-attempting to answer questions presented by the social ecological schema with practices developed in response to biomedicine. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3389/fpubh.2020.00131DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7221057PMC

Analysis of the Mouse Y Chromosome by Single-Molecule Sequencing With Y Chromosome Enrichment.

Front Genet 2020 7;11:406. Epub 2020 May 7.

Department of Systems BioMedicine, Tokyo Medical and Dental University, Tokyo, Japan.

Since human and mouse Y chromosomes contain repeated sequences, it is difficult to determine the precise sequences and analyze the function of individual Y chromosome genes. Therefore, the causes of many diseases and abnormalities related to Y chromosome genes, such as male infertility, remain unclear. In this study, to elucidate the mouse Y chromosome, we enriched the mouse Y chromosome using a fluorescence-activated cell sorter (FACS) equipped with commonly used UV and blue 488 nm lasers and read the nucleotides using the Oxford Nanopore MinION long-read sequencer. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3389/fgene.2020.00406DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7221202PMC

A Bitter Taste in Your Heart.

Front Physiol 2020 8;11:431. Epub 2020 May 8.

School of Biomedical Sciences, Faculty of Medicine, The University of Queensland, St Lucia, QLD, Australia.

The human genome contains ∼29 bitter taste receptors (T2Rs), which are responsible for detecting thousands of bitter ligands, including toxic and aversive compounds. This sentinel function varies between individuals and is underpinned by naturally occurring T2R polymorphisms, which have also been associated with disease. Recent studies have reported the expression of T2Rs and their downstream signaling components within non-gustatory tissues, including the heart. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3389/fphys.2020.00431DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7225360PMC

The Curcumin Derivative, H10, Suppresses Hormone-Dependent Prostate Cancer by Inhibiting 17β-Hydroxysteroid Dehydrogenase Type 3.

Front Pharmacol 2020 8;11:637. Epub 2020 May 8.

Institute of Biomedicine and Guangdong Provincial Key Laboratory of Bioengineering Medicine, Jinan University, Guangzhou, China.

The 17β-hydroxysteroid dehydrogenase type 3 (17β-HSD3) enzyme is a potential therapeutic target for hormone-dependent prostate cancer, as it is the key enzyme in the last step of testosterone (T) biosynthesis. A curcumin analog, H10, was optimized for inhibiting T production in LC540 cells that stably overexpressed 17β-HSD3 enzyme (LC540 [17β-HSD3]) (P < 0.01), without affecting progesterone (P) synthesis. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3389/fphar.2020.00637DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7227374PMC

Unity Makes Strength: Exploring Intraspecies and Interspecies Toxin Synergism between Phospholipases A and Cytotoxins.

Front Pharmacol 2020 7;11:611. Epub 2020 May 7.

Department of Biotechnology and Biomedicine, Technical University of Denmark, Kongens Lyngby, Denmark.

Toxin synergism is a complex biochemical phenomenon, where different animal venom proteins interact either directly or indirectly to potentiate toxicity to a level that is above the sum of the toxicities of the individual toxins. This provides the animals possessing venoms with synergistically enhanced toxicity with a metabolic advantage, since less venom is needed to inflict potent toxic effects in prey and predators. Among the toxins that are known for interacting synergistically are cytotoxins from snake venoms, phospholipases A from snake and bee venoms, and melittin from bee venom. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3389/fphar.2020.00611DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7221120PMC

A Hybrid Peptide DEFB-TP5 Expressed in Methylotrophic Yeast Neutralizes LPS With Potent Anti-inflammatory Activities.

Front Pharmacol 2020 7;11:461. Epub 2020 May 7.

State Key Laboratory of Animal Nutrition and Feed Sciences, Laboratory of Feed Biotechnology, College of Animal Science and Technology, China Agricultural University, Beijing, China.

DEFB-TP5 is a novel auspicious health-beneficial peptide derivative from two naturally occurring peptides, β-Defensin (DEFB) and thymopentin (TP5), and shows strong anti-inflammatory activity and binds to LPS without cytotoxicity and hemolytic effect. Furthermore, the application of DEFB-TP5 peptide is inadequate by its high cost. In the current study, we developed a biocompatible mechanism for expression of the DEFB-TP5 peptide in . Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3389/fphar.2020.00461DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7221121PMC

Cortical Inhibitory Imbalance in Functional Paralysis.

Front Hum Neurosci 2020 7;14:153. Epub 2020 May 7.

Stroke Unit, Azienda Socio Sanitaria Territoriale Spedali Civili, Brescia, Italy.

Background: Functional neurological disorders are characterized by neurological symptoms that have no identifiable pathology and little is known about their underlying pathophysiology.

Objectives: To analyze motor cortex excitability and intracortical inhibitory and excitatory circuits' imbalance in patients with flaccid functional weakness.

Methods: Twenty-one consecutive patients with acute onset of flaccid functional weakness were recruited. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3389/fnhum.2020.00153DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7220997PMC

Streamlined human antibody generation and optimization by exploiting designed immunoglobulin loci in a B cell line.

Cell Mol Immunol 2020 May 26. Epub 2020 May 26.

Research Laboratories, Chiome Bioscience Inc, Tokyo, Japan.

Monoclonal antibodies (mAbs) are widely utilized as therapeutic drugs for various diseases, such as cancer, autoimmune diseases, and infectious diseases. Using the avian-derived B cell line DT40, we previously developed an antibody display technology, namely, the ADLib system, which rapidly generates antigen-specific mAbs. Here, we report the development of a human version of the ADLib system and showcase the streamlined generation and optimization of functional human mAbs. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41423-020-0440-9DOI Listing

Repression of the lysogenic P promoter in bacteriophage TP901-1 through binding of a CI-MOR complex to a composite O-O operator.

Sci Rep 2020 May 26;10(1):8659. Epub 2020 May 26.

Technical University of Denmark, Department of Biotechnology and Biomedicine, Lyngby, DK2800, Denmark.

A functional genetic switch from the lactococcal bacteriophage TP901-1, deciding which of two divergently transcribing promoters becomes most active and allows this bi-stable decision to be inherited in future generations requires a DNA region of less than 1 kb. The fragment encodes two repressors, CI and MOR, transcribed from the P and P promoters respectively. CI can repress the transcription of the mor gene at three operator sites (O, O, and O), leading to the immune state. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41598-020-65493-0DOI Listing

The UK Biobank imaging enhancement of 100,000 participants: rationale, data collection, management and future directions.

Nat Commun 2020 May 26;11(1):2624. Epub 2020 May 26.

Nuffield Department of Population Health, University of Oxford, Oxford, UK.

UK Biobank is a population-based cohort of half a million participants aged 40-69 years recruited between 2006 and 2010. In 2014, UK Biobank started the world's largest multi-modal imaging study, with the aim of re-inviting 100,000 participants to undergo brain, cardiac and abdominal magnetic resonance imaging, dual-energy X-ray absorptiometry and carotid ultrasound. The combination of large-scale multi-modal imaging with extensive phenotypic and genetic data offers an unprecedented resource for scientists to conduct health-related research. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41467-020-15948-9DOI Listing

Predicting Phenotypic Polymyxin Resistance in Klebsiella pneumoniae through Machine Learning Analysis of Genomic Data.

mSystems 2020 May 26;5(3). Epub 2020 May 26.

Division of Infectious Diseases, Columbia University Irving Medical Center, New York, New York, USA

Polymyxins are used as treatments of last resort for Gram-negative bacterial infections. Their increased use has led to concerns about emerging polymyxin resistance (PR). Phenotypic polymyxin susceptibility testing is resource intensive and difficult to perform accurately. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1128/mSystems.00656-19DOI Listing

The Killing Mechanism of Teixobactin against Methicillin-Resistant Staphylococcus aureus: an Untargeted Metabolomics Study.

mSystems 2020 May 26;5(3). Epub 2020 May 26.

Department of Pharmacology and Therapeutics, School of Biomedical Sciences, Faculty of Medicine, Dentistry and Health Sciences, The University of Melbourne, Parkville, VIC, Australia

Antibiotics have served humankind through their use in modern medicine as effective treatments for otherwise fatal bacterial infections. Teixobactin is a first member of newly discovered natural antibiotics that was recently identified from a hitherto-unculturable soil bacterium, , and recognized as a potent antibacterial agent against various Gram-positive bacteria, including methicillin-resistant (MRSA) and vancomycin-resistant enterococci. The most distinctive characteristic of teixobactin as an effective antibiotic is that teixobactin resistance could not be evolved in a laboratory setting. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1128/mSystems.00077-20DOI Listing

Algal neurotoxin biosynthesis repurposes the terpene cyclase structural fold into an -prenyltransferase.

Proc Natl Acad Sci U S A 2020 May 26. Epub 2020 May 26.

Center for Marine Biotechnology and Biomedicine, Scripps Institution of Oceanography, University of California San Diego, La Jolla, CA 92093;

Prenylation is a common biological reaction in all domains of life wherein prenyl diphosphate donors transfer prenyl groups onto small molecules as well as large proteins. The enzymes that catalyze these reactions are structurally distinct from ubiquitous terpene cyclases that, instead, assemble terpenes via intramolecular rearrangements of a single substrate. Herein, we report the structure and molecular details of a new family of prenyltransferases from marine algae that repurposes the terpene cyclase structural fold for the -prenylation of glutamic acid during the biosynthesis of the potent neurochemicals domoic acid and kainic acid. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1073/pnas.2001325117DOI Listing

Perineuronal nets regulate the inhibitory perisomatic input onto parvalbumin interneurons and gamma activity in the prefrontal cortex.

J Neurosci 2020 May 25. Epub 2020 May 25.

Neurobiology Unit, Department of Cell Biology, Interdisciplinary Research Structure for Biotechnology and Biomedicine (BIOTECMED), University of Valencia, 46100, Spain.

Parvalbumin expressing (PV+) interneurons play a key role in the maturation and synchronization of cortical circuitry and alterations in these inhibitory neurons, especially in the medial prefrontal cortex (mPFC), have been found in different psychiatric disorders. The formation of perineuronal nets (PNNs) around many of these interneurons at the end of the critical periods reduces their plasticity and sets their connectivity. Consequently, the presence of PNNs must have an important impact on the synaptic input and the physiology of PV+ cells. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1523/JNEUROSCI.0291-20.2020DOI Listing

Smartphone based meat freshness detection.

Talanta 2020 Aug 3;216:120985. Epub 2020 Apr 3.

ECsens, Department of Analytical Chemistry, University of Granada, Granada, 18071, Spain; Unit of Excellence in Chemistry Applied to Biomedicine and the Environment of the University of Granada, Spain.

In this work, a freshness colorimetric sensor has been integrated with pork meat packages. The sensor tracks rising CO levels in the package associated with meat spoilage, as CO levels increase with bacterial population. The color of the sensor changes depending on the quantity of bacteria present, therefore it can be correlated with the freshness of meat, in this case pork loin. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.talanta.2020.120985DOI Listing

Lipid profile and left ventricular geometry pattern in obese children.

Lipids Health Dis 2020 May 26;19(1):109. Epub 2020 May 26.

Department of Hypertension, Chair of Nephrology and Hypertension, Medical University of Lodz, 281/289 Rzgowska St., 93-338, Lodz, Poland.

Background: Left ventricular hypertrophy (LVH) is an important risk factor for cardiovascular and all-cause mortality. Previous studies reported conflicting results concerning the relationship between serum lipid levels and left ventricular geometry pattern. We sought to explore the relationship between standard serum lipid profile measures with left ventricular geometry pattern in obese children. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1186/s12944-020-01285-9DOI Listing

Pro-Inflammatory Cytokines Are Modulated in Vietnamese Patients with Dengue Fever.

Viral Immunol 2020 May 22. Epub 2020 May 22.

Department of Pathophysiology, Vietnam Military Medical University, Hanoi, Vietnam.

The clinical outcome of dengue is due to a complex interplay between dengue virus (DENV) and host immune factors, including complement and cytokine systems. Pro-inflammatory cytokines are mainly produced by monocytes in response to infectious pathogens. This study investigated the levels of pro-inflammatory cytokines, including tumor necrosis factor-α (TNF-α), interleukin-1 beta (IL-1β), and IL-12 in Vietnamese patients with dengue, and their correlations with the clinical outcome of dengue infection in 156 patients clinically classified as dengue without warning signs (DWS-,  = 87), dengue with warning signs (DWS+,  = 62), and severe dengue (SD,  = 7) patients as well as in 60 healthy controls (HCs). Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1089/vim.2020.0023DOI Listing

An Insight into the Structural Diversity and Clinical Applicability of Polyurethanes in Biomedicine.

Polymers (Basel) 2020 May 24;12(5). Epub 2020 May 24.

Department of Cardiac Surgery, "Victor Babes" University of Medicine and Pharmacy Timisoara, 2 Eftimie Murgu sq, 300041 Timisoara, Romania.

Due to their mechanical properties, ranging from flexible to hard materials, polyurethanes (PUs) have been widely used in many industrial and biomedical applications. PUs' characteristics, along with their biocompatibility, make them successful biomaterials for short and medium-duration applications. The morphology of PUs includes two structural phases: hard and soft segments. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3390/polym12051197DOI Listing

Food Addiction in a Group of Italian Adolescents Diagnosed for Eating Disorder.

Nutrients 2020 May 23;12(5). Epub 2020 May 23.

Anorexia Nervosa and Eating Disorder Unit, Child Neuropsychiatry, Dept. of Neuroscience, Bambino Gesù Children Hospital IRCCS, 00165 Rome, Italy.

Research in patients with Eating Disorders (EDs) showed high rates of Food Addiction (FA) even in restrictive subtypes. The majority of studies were conducted on adult population. The present work aimed to describe and compared FA in adolescents diagnosed for different EDs and to evaluate its association with patients' psychopathology. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3390/nu12051524DOI Listing

Amelioration of BPSD-Like Phenotype and Cognitive Decline in SAMP8 Mice Model Accompanied by Molecular Changes after Treatment with I-Imidazoline Receptor Ligand MCR5.

Pharmaceutics 2020 May 23;12(5). Epub 2020 May 23.

Pharmacology Section, Department of Pharmacology, Toxicology and Medicinal Chemistry, Faculty of Pharmacy and Food Sciences, and Institut de Neurociències, University of Barcelona, Av. Joan XXIII, 27-31, E-08028 Barcelona, Spain.

Behavioural and psychological symptoms of dementia (BPSD), including fear-anxiety- and depressive-like behaviour, are present in Alzheimer's disease (AD), together with memory decline. I-imidazoline receptors (I-IRs) have been associated with neuropsychiatric and neurodegenerative disorders, further, I-IR ligands have demonstrated a neuroprotective role in the central nervous system (CNS). In this study, we assessed the effect of the I-IR ligand MCR5 on both cognitive and non-cognitive symptoms in the Senescence accelerated mice prone 8 (SAMP8) mouse model. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3390/pharmaceutics12050475DOI Listing

Comparative Analysis of ANDE 6C Rapid DNA Analysis System and Traditional Methods.

Genes (Basel) 2020 May 22;11(5). Epub 2020 May 22.

Department of Biomedicine and Prevention, Tor Vergata University of Rome, 00133 Rome, Italy.

Rapid DNA analysis is an ultrafast and fully automated DNA-typing system, which can produce interpretable genetic profiles from biological samples within 90 minutes. This "swab in-profile out" method comprises DNA extraction, amplification by PCR multiplex, separation and detection of DNA fragments by capillary electrophoresis. The aim of study was the validation of the Accelerated Nuclear DNA Equipment (ANDE) 6C system as a typing method for reference samples according to the ISO/IEC 17025 standard. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3390/genes11050582DOI Listing

Effect of Erufosine on Membrane Lipid Order in Breast Cancer Cell Models.

Biomolecules 2020 May 22;10(5). Epub 2020 May 22.

Institute of Biochemistry and Biology, University of Potsdam, Karl-Liebknecht-Street 24-25, 14476 Potsdam, Germany.

Alkylphospholipids are a novel class of antineoplastic drugs showing remarkable therapeutic potential. Among them, erufosine (EPC3) is a promising drug for the treatment of several types of tumors. While EPC3 is supposed to exert its function by interacting with lipid membranes, the exact molecular mechanisms involved are not known yet. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3390/biom10050802DOI Listing

Delayed NK Cell Reconstitution and Reduced NK Activity Increased the Risks of CMV Disease in Allogeneic-Hematopoietic Stem Cell Transplantation.

Int J Mol Sci 2020 May 22;21(10). Epub 2020 May 22.

Department of Laboratory Medicine, Seoul St. Mary's Hospital, College of Medicine, The Catholic University of Korea, Seoul 06591, Korea.

Cytomegalovirus (CMV) infection has a significant impact in patients after allogeneic hematopoietic stem cell transplantation (HSCT). We investigated natural killer (NK) cell reconstitution and cytotoxic/cytokine production in controlling CMV infection, especially severe CMV disease in HSCT patients. Fifty-eight patients with acute myeloid leukemia (AML) who received allo-HSCT were included. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3390/ijms21103663DOI Listing

Rafoxanide Induces Immunogenic Death of Colorectal Cancer Cells.

Cancers (Basel) 2020 May 21;12(5). Epub 2020 May 21.

Department of Systems Medicine, University of Rome "Tor Vergata", 00133 Rome, Italy.

Colorectal cancer (CRC) is a major cause of cancer-related death in the world. Emerging evidence suggests that the clinical success of conventional chemotherapy does not merely rely on cell toxicity, but also results from the restoration of tumor immune surveillance. Anti-tumor immune response can be primed by immunogenic cell death (ICD), a form of apoptosis associated with endoplasmic reticulum stress (ERS) induction and the expression/release of specific damage-associated molecular patterns (DAMPs). Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3390/cancers12051314DOI Listing

ASC-Exosomes Ameliorate the Disease Progression in SOD1(G93A) Murine Model Underlining Their Potential Therapeutic Use in Human ALS.

Int J Mol Sci 2020 May 21;21(10). Epub 2020 May 21.

Department of Neuroscience, Biomedicine and Movement Sciences, University of Verona, 37134 Verona, Italy.

Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disease characterized by progressive degeneration of motoneurons. To date, there is no effective treatment available. Exosomes are extracellular vesicles that play important roles in intercellular communication, recapitulating the effect of origin cells. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3390/ijms21103651DOI Listing

First Isolation and Characterization of SKNUCL01, Isolated from Diseased Pond loach () in Korea.

Pathogens 2020 May 21;9(5). Epub 2020 May 21.

Laboratory of Aquatic Biomedicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 08826, Korea.

Loaches are widely distributed throughout the natural environment and are consumed for medicinal purposes in East Asia. Usually, loaches are cultured in ponds where the water conditions can easily cause bacterial infections. Infections due to bacterial pathogens such as have been well described in cultured loaches; however, there is no report regarding infection. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3390/pathogens9050397DOI Listing

Phenotypic Heterogeneity and Plasticity of Cancer Cell Migration in a Pancreatic Tumor Three-Dimensional Culture Model.

Cancers (Basel) 2020 May 21;12(5). Epub 2020 May 21.

Department of Biomedicine & Health Sciences, Graduate School, The Catholic University of Korea, Seoul 06591, Korea.

Invasive cancer cell migration is a key feature of metastatic human pancreatic ductal adenocarcinoma (PDAC), yet the underlying mechanisms remain poorly understood. Here, we investigated modes of cancer cell invasion using two pancreatic cancer cell lines with differential epithelial-mesenchymal status, PANC-1 and BxPC-3, under 3D culture conditions. Multicellular tumor spheroids (TSs) were grown in a collagen matrix co-cultured with pancreatic stellate cells (PSCs) using microchannel chips. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3390/cancers12051305DOI Listing

Preoperative, Intraoperative and Postoperative Corticosteroid Use as an Adjunctive Treatment for Rhegmatogenous Retinal Detachment.

J Clin Med 2020 May 21;9(5). Epub 2020 May 21.

Department of Ophthalmology, University of Catania, 95100 Catania, Italy.

The treatment for rhegmatogenous retinal detachment (RRD) is surgery, including pars plana vitrectomy (PPV) and scleral buckling (SB). Despite surgical advances, degeneration of the photoreceptors and post-operative complications, such as proliferative vitreoretinopathy (PVR), often occurs as the result of inflammation, preventing complete visual recovery or causing RRD recurrence. There is increasing evidence that in the presence of RRD, the activation of inflammatory processes occurs and the surgery itself induces an inflammatory response. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3390/jcm9051556DOI Listing

Sex Differences in Association of Elevated Blood Pressure with Variables Characterizing Cardiometabolic Risk in Young Subjects with or Without Metabolic Abnormalities.

Int J Environ Res Public Health 2020 May 21;17(10). Epub 2020 May 21.

Institute of Materials & Machine Mechanics, Slovak Academy of Sciences, 845 13 Bratislava, Slovakia.

Males present higher blood pressure (BP) values, higher prevalence of elevated BP, and a different prevalence of cardiometabolic risk factors when compared with females. We assumed that the trends of risk markers across BP categories (normotension, high normal BP, and hypertension) differ in young males and females, and between subjects without metabolic abnormalities (without obesity, insulin resistance, atherogenic dyslipidemia, hyperuricemia, or microinflammation) and those presenting them. Data from 2543 subjects (48% males) aged from 16 to 23 years were analyzed. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3390/ijerph17103612DOI Listing

Platelets in Thrombo-Inflammation: Concepts, Mechanisms, and Therapeutic Strategies for Ischemic Stroke.

Hamostaseologie 2020 Jun 26;40(2):153-164. Epub 2020 May 26.

Institute of Experimental Biomedicine, University Hospital and Rudolf Virchow Center, University of Würzburg, Würzburg, Germany.

Platelets are anucleate cells known for their essential function in hemostasis and formation of thrombi under pathologic conditions. In recent years, strong evidence emerged demonstrating the critical involvement of platelets in inflammatory processes including acute ischemic stroke (AIS), which is one of the leading causes of death and disability worldwide. Recanalization of the occluded brain artery to reconstitute cerebral blood flow is the primary goal in the treatment of stroke patients. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1055/a-1151-9519DOI Listing

Gut neurotoxin p-cresol induces differential expression of GLUN2B and GLUN2A subunits of the NMDA receptor in the hippocampus and nucleus accumbens in healthy and audiogenic seizure-prone rats.

AIMS Neurosci 2020 10;7(1):30-42. Epub 2020 Mar 10.

Institute of Chemical Biology, Ilia State University, 3/5 Cholokashvili av, Tbilisi, 0162, Georgia.

Mislocalization and abnormal expression of N-methyl-D-aspartate glutamate receptor (NMDAR) subunits is observed in several brain disorders and pathological conditions. Recently, we have shown that intraperitoneal injection of the gut neurotoxin p-cresol induces autism-like behavior and accelerates seizure reactions in healthy and epilepsy-prone rats, respectively. In this study, we evaluated the expression of GLUN2B and GLUN2A NMDAR subunits, and assessed the activity of cAMP-response element binding protein (CREB) and Rac1 in the hippocampi and nucleus accumbens of healthy and epilepsy-prone rats following p-cresol administration. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3934/Neuroscience.2020003DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7242059PMC

Non-surgical oral hygiene interventions on disease activity of Rheumatoid arthritis patients with periodontitis: A randomized controlled trial.

J Dent Res Dent Clin Dent Prospects 2020 ;14(1):26-36

Department of Medicine, School of Medicine, Makerere University College of Health Sciences, Kampala Uganda.

Periodontitis and rheumatoid arthritis have similar epidemiology and pathophysiology. Understanding the interaction between these two diseases is vital in our settings. We set out to assess the effect of oral hygiene interventions on disease activity of rheumatoid arthritis patients with periodontitis in Kampala, Uganda. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.34172/joddd.2020.004DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7235694PMC
January 2020

Effect of Supplementation on Oxidative Stress and Antioxidant Parameters: A Meta-Analysis of Randomized Controlled Trials.

ScientificWorldJournal 2020 6;2020:2390706. Epub 2020 May 6.

Medical Doctoral Program Student, Faculty of Medicine, Surabaya, Indonesia.

Introduction: is a commonly used traditional medicine which has been shown to have antioxidant properties. However, its supplementation in patients of clinical trials showed conflicting results. . Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1155/2020/2390706DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7225850PMC