574,140 results match your criteria Biomedical papers of the Medical Faculty of the University Palacky Olomouc Czechoslovakia[Journal]


Factor XIII Prevents Pulmonary Emboli in Mice by Stabilizing Deep Vein Thrombi.

Thromb Haemost 2019 Apr 20. Epub 2019 Apr 20.

Thrombosis and Atherosclerosis Research Institute, McMaster University, Hamilton, Ontario, Canada.

Background:  Deep vein thrombosis (DVT) can lead to pulmonary embolism (PE), but the mechanisms responsible for this progression are unknown. Previously, we showed that inhibition of thrombin-mediated activation of factor (F) XIII promotes venous thrombus stability in a murine model.

Aim:  In this study, we investigate the consequence of attenuating fibrinolysis, using FXIII, α-antiplasmin (α-AP) or ε-aminocaproic acid (EACA) supplementation, on clot lysis and venous thrombus stability using the same mouse model. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1055/s-0039-1685141DOI Listing
April 2019
1 Read

An updated European Organisation for Research and Treatment of Cancer (EORTC) protocol for pathological evaluation of sentinel lymph nodes for melanoma.

Eur J Cancer 2019 Apr 17;114:1-7. Epub 2019 Apr 17.

Department of Surgical Oncology, Netherlands Cancer Institute Antoni van Leeuwenhoek, Amsterdam, the Netherlands.

The sentinel lymph node (SLN) biopsy is a highly accurate staging procedure and the most important prognostic factor in melanoma patients. The European Organisation for Research and Treatment of Cancer (EORTC) Melanoma Group aimed to design an updated evolved SLN protocol for the histopathological workup and reporting. We herein recommend extending the distance between steps according to the short axis dimension of the lymph node and optimise both conventional sectioning and staining procedures including immunohistochemistry. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ejca.2019.03.010DOI Listing

Multiscale modeling of the cardiovascular system for infants, children, and adolescents: Age-related alterations in cardiovascular parameters and hemodynamics.

Comput Biol Med 2019 Mar 23;108:200-212. Epub 2019 Mar 23.

Graduate School of Engineering, Chiba University, Inage, Chiba, Japan; Shanghai Jiao Tong University and Chiba University International Cooperative Research Center (SJTU-CU ICRC), Minhang District, Shanghai, China. Electronic address:

While zero- and one-dimensional multiscale modeling of the adult cardiovascular system (CVS) has been recognized as a useful tool in cardiovascular research and clinical applications, there are still not any generic cardiovascular models for a broad range of age groups. To study age-related alterations in cardiovascular parameters and hemodynamics, we here presented a versatile multiscale cardiovascular model considering the cardiovascular growth and development during aging. An adult cardiovascular model was first established by utilizing population-averaged physiological data. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.compbiomed.2019.03.021DOI Listing

A multi-scale data fusion framework for bone age assessment with convolutional neural networks.

Comput Biol Med 2019 Mar 19;108:161-173. Epub 2019 Mar 19.

Department of Electrical and Computer Engineering, University of British Columbia, Vancouver, BC, Canada.

Bone age assessment (BAA) has various clinical applications such as diagnosis of endocrine disorders and prediction of final adult height for adolescents. Recent studies indicate that deep learning techniques have great potential in developing automated BAA methods with significant advantages over the conventional methods based on handcrafted features. In this paper, we propose a multi-scale data fusion framework for bone age assessment with X-ray images based on non-subsampled contourlet transform (NSCT) and convolutional neural networks (CNNs). Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.compbiomed.2019.03.015DOI Listing

A novel end-to-end brain tumor segmentation method using improved fully convolutional networks.

Comput Biol Med 2019 Mar 18;108:150-160. Epub 2019 Mar 18.

School of Information Science and Technology, and Centers for Biomedical Engineering, University of Science and Technology of China, Hefei, AH 230027, China.

Accurate brain magnetic resonance imaging (MRI) tumor segmentation continues to be an active research topic in medical image analysis since it provides doctors with meaningful and reliable quantitative information in diagnosing and monitoring neurological diseases. Successful deep learning-based proposals have been designed, and most of them are built upon image patches. In this paper, a novel end-to-end brain tumor segmentation method is developed using an improved fully convolutional network by modifying the U-Net architecture. Read More

View Article

Download full-text PDF

Source
https://linkinghub.elsevier.com/retrieve/pii/S00104825193008
Publisher Site
http://dx.doi.org/10.1016/j.compbiomed.2019.03.014DOI Listing
March 2019
2 Reads

Racial disparities in postoperative respiratory failure after carotid endarterectomy.

J Clin Anesth 2019 Apr 17;57:139-140. Epub 2019 Apr 17.

Department of Anesthesiology, University of California San Diego, La Jolla, CA, USA; Department of Medicine, Division of Biomedical Informatics, University of California San Diego, CA, USA.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jclinane.2019.04.015DOI Listing

Imatinib-induced changes in the expression profile of microRNA in the plasma and heart of mice-A comparison with doxorubicin.

Biomed Pharmacother 2019 Apr 17;115:108883. Epub 2019 Apr 17.

Faculty of Pharmacy, Charles University, Heyrovského 1203, Hradec Králové, Czech Republic. Electronic address:

Cardiotoxicity is a serious adverse reaction to cancer chemotherapy and may lead to critical heart damage. Imatinib mesylate (IMB), a selective tyrosine kinase inhibitor, is sometimes accompanied by severe cardiovascular complications. To minimize risk, early biomarkers of such complications are of utmost importance. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.biopha.2019.108883DOI Listing

F-FDG PET/MR-imaging in a Göttingen Minipig model of atherosclerosis: Correlations with histology and quantitative gene expression.

Atherosclerosis 2019 Apr 9;285:55-63. Epub 2019 Apr 9.

Department of Clinical Physiology, Nuclear Medicine & PET and Cluster for Molecular Imaging, Dept. of Biomedical Sciences, Rigshospitalet and University of Copenhagen, Blegdamsvej 9, DK-2100, Copenhagen, Denmark. Electronic address:

Background And Aims: The advantage of combining molecular and morphological imaging, e.g. positron emission tomography and magnetic resonance imaging (PET/MRI), is reflected in the increased use of these modalities as surrogate end-points in clinical trials. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.atherosclerosis.2019.04.209DOI Listing

Permselective glucose sensing with GLUT1-rich cancer cell membranes.

Biosens Bioelectron 2019 Apr 12;135:82-87. Epub 2019 Apr 12.

School of Biomedical Engineering, Korea University, Seoul, 02841, Republic of Korea. Electronic address:

Enzymatic blood glucose detection with selectivity is one of the most important conundrums, because human blood contains many components that can hinder enzyme-substrate reactions. Meanwhile, cancer cells express much higher levels of glucose transporter-1 on their cell membrane to selectively and excessively uptake more α-D-glucose than do normal cells. Inspired by such cellular permselectivity for glucose, herein we significantly improved the selectivity of a glucose sensor by using a breast cancer cell membrane (BCCM). Read More

View Article

Download full-text PDF

Source
https://linkinghub.elsevier.com/retrieve/pii/S09565663193028
Publisher Site
http://dx.doi.org/10.1016/j.bios.2019.04.007DOI Listing
April 2019
1 Read
6.409 Impact Factor

Neotropical wild rodents Akodon and Oligoryzomys (Cricetidae: Sigmodontinae) as important carriers of pathogenic renal Leptospira in the Atlantic forest, in Brazil.

Res Vet Sci 2019 Apr 11;124:280-283. Epub 2019 Apr 11.

Fluminense Federal University, Laboratory of Veterinary Bacteriology, Biomedical Institute, 101 Prof. Hernani Mello Street, Niterói, Rio de Janeiro, Brazil. Electronic address:

Leptospirosis is an important worldwide zoonosis, caused by a bacterium of the genus Leptospira. For a better understanding of the disease, it is relevant the application of the one health concept. The Atlantic Forest is considered a biodiversity hotspot, with a great endemism of species and despite its importance and proximity to urban areas, the potential role of its fauna as carriers of infectious agents is still poorly understood. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.rvsc.2019.04.001DOI Listing

Exploring the role of BDNF DNA methylation and hydroxymethylation in patients with obsessive compulsive disorder.

J Psychiatr Res 2019 Apr 6;114:17-23. Epub 2019 Apr 6.

University of Milan, Department of Biomedical and Clinical Sciences Luigi Sacco, Milano, Italy; CRC "Aldo Ravelli", University of Milan, Milano, Italy; Department of Psychiatry and Behavioral Sciences, Stanford University, CA, USA. Electronic address:

Obsessive-compulsive disorder (OCD) is a clinically heterogeneous neuropsychiatric condition associated with profound disability, whose susceptibility, stemming from genetic and environmental factors that intersect with each other, is still under investigation. In this perspective, we sought to explore the transcriptional regulation of Brain Derived Neurotrophic Factor (BDNF), a promising candidate biomarker in both development and etiology of different neuropsychiatric conditions, in peripheral blood mononuclear cells from OCD patients and healthy controls. In particular, we focused on BDNF gene expression and interrogated in depth DNA methylation and hydroxymethylation at gene promoters (exons I, IV and IX) in a sample of OCD patients attending a tertiary OCD Clinic to receive guidelines-recommended treatment, and matched controls. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jpsychires.2019.04.006DOI Listing

Emodin improves glucose metabolism by targeting microRNA-20b in insulin-resistant skeletal muscle.

Phytomedicine 2018 Nov 16;59:152758. Epub 2018 Nov 16.

Department of Pharmacology (State-Province Key Laboratories of Biomedicine-Pharmaceutics of China, Key Laboratory of Cardiovascular Research, Ministry of Education), Harbin Medical University, Harbin 150081, China; Department of Pharmacology and Therapeutics, Melbourne School of Biomedical Sciences, Faculty of Medicine, Dentistry and Health Sciences, the University of Melbourne, Melbourne, 3010, Australia. Electronic address:

Background: Emerging evidence has indicated the therapeutic potential of emodin with its multiple pharmacological effects.

Purpose: To evaluate role of emodin in regulating insulin resistance (IR) and to elucidate the underlying molecular mechanisms.

Study Design/methods: Fasting blood glucose (FBG) and lipid levels were measured before and after intragastric administration of emodin in type 2 diabetes mellitus (T2DM) rats. Read More

View Article

Download full-text PDF

Source
https://linkinghub.elsevier.com/retrieve/pii/S09447113183057
Publisher Site
http://dx.doi.org/10.1016/j.phymed.2018.11.018DOI Listing
November 2018
1 Read

A Microparticle Approach for Non-viral Gene Delivery within 3D Human Mesenchymal Stromal Cell Aggregates.

Acta Biomater 2019 Apr 17. Epub 2019 Apr 17.

Department of Biomedical Engineering, University of Wisconsin, Madison, WI 53706, USA; Materials Science Program, University of Wisconsin, Madison, WI 53706, USA; Department of Orthopedics and Rehabilitation, University of Wisconsin, Madison, WI 53705, USA; AO Foundation Collaborative Research Center, Davos, Switzerland. Electronic address:

Three-dimensional (3D) multicellular aggregates, in comparison to two-dimensional monolayer culture, can provide tissue culture models that better recapitulate the abundant cell-cell and cell-matrix interactions found in vivo. In addition, aggregates are potentially useful building blocks for tissue engineering. However, control over the interior aggregate microenvironment is challenging due to inherent barriers for diffusion of biological mediators (e. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.actbio.2019.04.038DOI Listing

Microstructured Hydrogel Scaffolds Containing Differential Density Interfaces Promote Rapid Cellular Invasion and Vascularization.

Acta Biomater 2019 Apr 17. Epub 2019 Apr 17.

Laboratory of Bioregenerative Medicine & Surgery, Division of Plastic Surgery, Weill Cornell Medical Center, 1300 York, Room A-821, New York, NY 10021, United States; Nancy E. and Peter C. Meinig School of Biomedical Engineering, Cornell University, 121A Weill Hall, Ithaca, NY 14853, United States. Electronic address:

Introduction: Insufficient vascularization of currently available clinical biomaterials has limited their application to optimal wound beds. We designed a hydrogel scaffold with a unique internal microstructure of differential collagen densities to induce cellular invasion and neovascularization.

Methods: Microsphere scaffolds (MSS) were fabricated by encasing 1% (w/v) type 1 collagen microspheres 50-150 μm in diameter in 0. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.actbio.2019.04.027DOI Listing

Integration of biological systems with electronic-mechanical assemblies.

Acta Biomater 2019 Apr 17. Epub 2019 Apr 17.

Department of Materials Science and Engineering, The Pennsylvania State University, University Park, Pennsylvania 16802, USA; Department of Engineering Science and Mechanics, and Materials Research Institute, The Pennsylvania State University, University Park, Pennsylvania 16802, USA. Electronic address:

Biological systems continuously interact with the surrounding environment because they are dynamically evolving. The interaction is achieved through mechanical, electrical, chemical, biological, thermal, optical, or a synergistic combination of these cues. To provide a fundamental understanding of the interaction, recent efforts that integrate biological systems with the electronic-mechanical assemblies create unique opportunities for simultaneous monitoring and eliciting the responses to the biological system. Read More

View Article

Download full-text PDF

Source
https://linkinghub.elsevier.com/retrieve/pii/S17427061193027
Publisher Site
http://dx.doi.org/10.1016/j.actbio.2019.04.032DOI Listing
April 2019
1 Read

3D light-sheet assay assessing novel valproate-associated cardiotoxicity and folic acid relief in zebrafish embryogenesis.

Chemosphere 2019 Apr 11;227:551-560. Epub 2019 Apr 11.

Division of Biomedical Convergence, College of Biomedical Science, Kangwon National University, Chuncheon, South Korea. Electronic address:

Precise in vivo toxicological assays to determine the cardiotoxicity of pharmaceuticals and their waste products are essential in order to evaluate their risks to humans and the environment following industrial release. In the present study, we aimed to develop the sensitive imaging-based cardiotoxicity assay and combined 3D light-sheet microscopy with a zebrafish model to identify hidden cardiovascular anomalies induced by valproic acid (VPA) exposure. The zebrafish model is advantageous for this assessment because its embryos remain transparent. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.chemosphere.2019.04.061DOI Listing

Rare earth elements regulate the endocytosis and DNA methylation in root cells of Arabidopsis thaliana.

Chemosphere 2019 Apr 11;227:522-532. Epub 2019 Apr 11.

National and Local Joint Engineering Research Center of Biomedical Functional Materials, Jiangsu Collaborative Innovation Center of Biomedical Functional Materials, School of Chemistry and Materials Science, Nanjing Normal University, Nanjing, 210023, China. Electronic address:

With increasing application of rare earth elements (REEs), the resulting environmental safety has attracted extensive attention. When REEs act on plant leaves, REEs can initiate endocytosis in leaf cells, causing more REEs enter plant cells and then severe damage to plants. But when REEs directly act on plant roots, whether and how REEs affect the endocytosis in root cells remain unknown. Read More

View Article

Download full-text PDF

Source
https://linkinghub.elsevier.com/retrieve/pii/S00456535193072
Publisher Site
http://dx.doi.org/10.1016/j.chemosphere.2019.04.076DOI Listing
April 2019
1 Read

3D printed scaffolds with hierarchical biomimetic structure for osteochondral regeneration.

Nanomedicine 2019 Apr 17. Epub 2019 Apr 17.

Department of Mechanical and Aerospace Engineering, The George Washington University, Washington, DC 20052, USA; Department of Biomedical Engineering, The George Washington University, Washington, DC 20052, USA; Department of Electrical and Computer Engineering, The George Washington University, Washington, DC 20052, USA; Department of Medicine, The George Washington University, Washington, DC 20052, USA. Electronic address:

Osteochondral defects resulting from trauma and/or pathologic disorders are critical clinical problems. The current approaches still do not yield satisfactory due to insufficient donor sources and potential immunological rejection of implanted tissues. 3D printing technology has shown great promise for fabricating customizable, biomimetic tissue matrices. Read More

View Article

Download full-text PDF

Source
https://linkinghub.elsevier.com/retrieve/pii/S15499634193008
Publisher Site
http://dx.doi.org/10.1016/j.nano.2019.04.002DOI Listing
April 2019
1 Read

Differential Effects of Unipolar versus Bipolar Depression on Episodic Memory Updating.

Neurobiol Learn Mem 2019 Apr 17. Epub 2019 Apr 17.

National Center for Biological Sciences, Tata Institute of Fundamental Research, Bangalore 560065, India; Centre for Brain Development and Repair, Institute for Stem Cell Biology and Regenerative Medicine, Bangalore 560065, India; Centre for Discovery Brain Sciences, Deanery of Biomedical Sciences, University of Edinburgh, Hugh Robson Building, 15 George Square, Edinburgh EH89XD, UK.

Episodic memories, when reactivated, can be modified or updated by new learning. Since such dynamic memory processes remain largely unexplored in psychiatric disorders, we examined the impact of depression on episodic memory updating. Unipolar and bipolar depression patients, and age/education matched controls, first learned a set of objects (List-1). Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.nlm.2019.04.008DOI Listing

Impact of the replacement of dietary fish oil by animal fats and environmental salinity on the metabolic response of European Seabass (Dicentrarchus labrax).

Comp Biochem Physiol B Biochem Mol Biol 2019 Apr 17. Epub 2019 Apr 17.

CIIMAR-Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Portugal. Electronic address:

The replacement of fish oil (FO) with other lipid sources (e.g. animal fats, AF) in aquafeeds improves the sustainability of aquaculture, even though alternatives have different fatty acid (FA) profiles. Read More

View Article

Download full-text PDF

Source
https://linkinghub.elsevier.com/retrieve/pii/S10964959183032
Publisher Site
http://dx.doi.org/10.1016/j.cbpb.2019.04.004DOI Listing
April 2019
1 Read

Human Erythroblasts with c-Kit Activating Mutations Have Reduced Cell Culture Costs and Remain Capable of Terminal Maturation.

Exp Hematol 2019 Apr 17. Epub 2019 Apr 17.

Center for Pediatric Biomedical Research, Department of Pediatrics, University of Rochester, Rochester, NY. Electronic address:

A major barrier to the in vitro production of red blood cells for transfusion therapy is the cost of culture components, with cytokines making up greater than half of the culture costs. Cell culture cytokines also represent a major expense for in vitro studies of human erythropoiesis. HUDEP2 cells are an E6/E7 immortalized erythroblast line used for the in vitro study of human erythropoiesis. Read More

View Article

Download full-text PDF

Source
https://linkinghub.elsevier.com/retrieve/pii/S0301472X193013
Publisher Site
http://dx.doi.org/10.1016/j.exphem.2019.04.001DOI Listing
April 2019
4 Reads

Transcriptional activation of SIRT6 via FKHRL1/FOXO3a inhibits the Warburg effect in glioblastoma cells.

Cell Signal 2019 Apr 17. Epub 2019 Apr 17.

State Key Laboratory of Silkworm Genome Biology, Institute of Sericulture and Systems Biology, Southwest University, Beibei, Chongqing 400716, China; Engineering Research Center for Cancer Biomedical and Translational Medicine, Southwest University, Beibei, Chongqing 400716, China; Chongqing Engineering and Technology Research Center for Silk Biomaterials and Regenerative Medicine, Southwest University, Beibei, Chongqing 400716, China; Cancer Center, Medical Research Institute, Southwest University, Beibei, Chongqing, China. Electronic address:

Glioblastoma (GBM) is the most aggressive and malignant form of brain tumors. However, its molecular mechanisms of tumorigenesis and cancer development remained to elucidate. Here, we reported FKHRL1, also called as FOXO3a, was an anti-cancer factor that inhibited the Warburg effect in GBM. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.cellsig.2019.04.009DOI Listing

Open-source pipeline for multi-class segmentation of the spinal cord with deep learning.

Magn Reson Imaging 2019 Apr 17. Epub 2019 Apr 17.

NeuroPoly Lab, Institute of Biomedical Engineering, Polytechnique Montreal, Montreal, QC, Canada; Functional Neuroimaging Unit, CRIUGM, Université de Montréal, Montreal, QC, Canada.

This paper presents an open-source pipeline to train neural networks to segment structures of interest from MRI data. The pipeline is tailored towards homogeneous datasets and requires relatively low amounts of manual segmentations (few dozen, or less depending on the homogeneity of the dataset). Two use-case scenarios for segmenting the spinal cord white and grey matter are presented: one in marmosets with variable numbers of lesions, and the other in the publicly available human grey matter segmentation challenge (Prados et al. Read More

View Article

Download full-text PDF

Source
https://linkinghub.elsevier.com/retrieve/pii/S0730725X193000
Publisher Site
http://dx.doi.org/10.1016/j.mri.2019.04.009DOI Listing
April 2019
1 Read

Neurophysiological Abnormalities in Individuals with Persistent Post-Concussion Symptoms.

Neuroscience 2019 Apr 17. Epub 2019 Apr 17.

Sports Performance Research Institute New Zealand (SPRINZ), Faculty of Health and Environmental Science Auckland University of Technology, Auckland, New Zealand.

Concussion injury results in a rapid onset of transient neurological impairment that can resolve quickly, or sometimes evolve over time, but usually resolve within seven to ten days. However, a small but noticeable cohort (~10%) of individuals continue to experience persistent lingering effects, particularly fatigue, and recognized as post-concussion symptoms (PCS). This study explored neurophysiological mechanisms in people with persistent PCS. Read More

View Article

Download full-text PDF

Source
https://linkinghub.elsevier.com/retrieve/pii/S03064522193025
Publisher Site
http://dx.doi.org/10.1016/j.neuroscience.2019.04.019DOI Listing
April 2019
1 Read

A structural prospective for collagen receptors such as DDR and their binding of the collagen fibril.

Biochim Biophys Acta Mol Cell Res 2019 Apr 17. Epub 2019 Apr 17.

Departments of Biology and Biomedical Engineering, Illinois Institute of Technology, Chicago, IL, USA.

The structure of the collagen fibril surface directly effects and possibly assists the management of, collagen receptor interactions. An important class of collagen receptors, the receptor tyrosine kinases of the Discoidin Domain Receptor family (DDR1 and DDR2), are differentially activated by specific collagen types and play important roles in cell adhesion, migration, proliferation, and matrix remodeling. This review discusses their structure and function as it pertains directly to the fibrillar collagen structure with which they interact far more readily than they do with isolated molecular collagen. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bbamcr.2019.04.008DOI Listing

Characterizing the protein corona of sub-10 nm nanoparticles.

J Control Release 2019 Apr 17. Epub 2019 Apr 17.

Department of Chemistry, University of Toronto, 80 St. George Street, Toronto, ON M5S 3H6, Canada; Terrence Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, 160 College Street, Room 230, Toronto, ON M5S 3E1, Canada; Institute of Biomaterials and Biomedical Engineering, University of Toronto, 164 College St, Toronto, ON M5S 3G9, Canada; Department of Chemical Engineering, University of Toronto, 200 College Street, Toronto, ON M5S 3E5, Canada; Department of Material Science and Engineering, University of Toronto, 160 College Street, Room 450, Toronto M5S 3E1, Canada. Electronic address:

Studies into the interactions of serum proteins with nanoparticles are typically performed using nanoparticles that are larger than the size of proteins. Due to this size discrepancy, adsorbed proteins are commonly depicted as a globular structure surrounding a nanoparticle. Here, we asked how we should view nanoparticle-protein complexes when the nanoparticles are of similar size or smaller than the proteins with which they interact. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jconrel.2019.04.023DOI Listing

Bio-assay: The best alternative for conventional methods in detection of epidermal growth factor.

Int J Biol Macromol 2019 Apr 17. Epub 2019 Apr 17.

Nanotechnology Research Center, Uremia University, Uremia, Iran.

Growth factors are necessary for proper and efficient wound closure and tissue regeneration. Epidermal growth factor (EGF) is one of the key signaling molecules in stimulating epithelial cell motility, making it a required factor for re-epithelialization. Increased EGF expression is likely to be a strong prognostic and predictive feature in multiple tumor types and determination of EGF may product remarkable diagnosis benefits. Read More

View Article

Download full-text PDF

Source
https://linkinghub.elsevier.com/retrieve/pii/S01418130193085
Publisher Site
http://dx.doi.org/10.1016/j.ijbiomac.2019.04.121DOI Listing
April 2019
1 Read

Polysaccharide and polypeptide based injectable thermo-sensitive hydrogels for local biomedical applications.

Int J Biol Macromol 2019 Apr 17. Epub 2019 Apr 17.

Graduate Institute of Applied Science and Technology, National Taiwan University of Science and Technology, Taipei 106, Taiwan; Advanced Membrane Materials Research Center, National Taiwan University of Science and Technology, Taipei 106, Taiwan; R&D Center for Membrane Technology, Chung Yuan Christian University, Chungli, Taoyuan 320, Taiwan.

Injectable thermo-responsive hydrogels have been studied for various biomedical applications including; drug delivery, cell encapsulation, and tissue repairing. There are various natural and synthetic polymers which exhibit homogenous injectable solution at ambient temperature and form a gel in human body temperature. Polysaccharide, polypeptide and other biological macromolecule based hydrogels have been getting immense attention in biomedical application due to their low immunogenicity, abundance in nature, high biocompatibility and biodegradability. Read More

View Article

Download full-text PDF

Source
https://linkinghub.elsevier.com/retrieve/pii/S01418130193198
Publisher Site
http://dx.doi.org/10.1016/j.ijbiomac.2019.04.131DOI Listing
April 2019
1 Read

Influence of swabbing solution and swab type on DNA recovery from rigid environmental surfaces.

J Microbiol Methods 2019 Apr 17. Epub 2019 Apr 17.

Department of Senior Healthcare, BK21 Plus Program, Graduate School, Eulji University, 77 Gyeryong-ro, 771 beon-gil, Jung-gu, Deajeon 34824, Republic of Korea; Department of Biomedical Laboratory Science, Eulji University, School of Medicine, 77 Gyeryong-ro, 771 beon-gil, Jung-gu, Deajeon 34824, Republic of Korea. Electronic address:

Determination of the metagenome has become an important component of forensic identification, which requires efficient environmental sampling techniques. Therefore, in this study, we compared the efficiency of sample collection using swabbing with cotton swabs and three types of medical swabs (S7, S22, S24) along with three different solutions: phosphate-buffered saline (PBS), 1% Tween 20 + 1% glycerol in PBS (TG), and GS commercial solution (Noble Bio, Hwaseong, Republic of Korea). Combinations of the three solutions with the three types of swabs were tested at different volumes (cotton swab, S7: 0, 30, 50, 70 μL; S22, S24: 0, 70, 100, 130 μL). Read More

View Article

Download full-text PDF

Source
https://linkinghub.elsevier.com/retrieve/pii/S01677012193020
Publisher Site
http://dx.doi.org/10.1016/j.mimet.2019.04.011DOI Listing
April 2019
1 Read

A contemporary biological pathway of islet amyloid polypeptide for the management of diabetic dementia.

Chem Biol Interact 2019 Apr 17. Epub 2019 Apr 17.

Discipline of Pharmacy, Graduate School of Health, University of Technology Sydney (UTS), Ultimo, NSW 2007, Australia; Priority Research Centre for Healthy Lungs, Hunter Medical Research Institute (HMRI) & School of Biomedical Sciences and Pharmacy, The University of Newcastle (UoN), Callaghan, NSW 2308, Australia. Electronic address:

Major challenges of dealing elder patients with diabetes mellitus (DM) are the individualization of consideration in persons with various comorbid types of conditions. In spite of the fact that microvascular and macrovascular problems associated with DM are well documented, there is only a few numbers of reports viewing different conditions, for example, cognitive dysfunction. Cognitive dysfunction is of specific significance due to its effect on self-care and quality of life. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.cbi.2019.04.022DOI Listing

Butyrylcholinesterase inhibited by nerve agents is efficiently reactivated with chlorinated pyridinium oximes.

Chem Biol Interact 2019 Apr 17. Epub 2019 Apr 17.

Institute for Medical Research and Occupational Health, Ksaverska cesta 2, HR-10000 Zagreb, Croatia. Electronic address:

Bispyridinium oximes with one (K865, K866, K867) or two (K868, K869, K870) ortho-positioned chlorine moiety, analogous to previously known K027, K048 and K203 oximes, and potent reactivators of human acetylcholinesterase (AChE) inhibited by nerve agents, were tested in the reactivation of human butyrylcholinesterase (BChE) inhibited by sarin, cyclosarin, VX, and tabun. A previously highlighted AChE reactivator, dichlorinated bispyridinium oxime with propyl linker (K868), was tested in more detail for reactivation of four nerve agent-BChE conjugates. Its BChE reactivation potency was showed to be promising when compared to the standard oximes used in medical practice, asoxime (HI-6) and pralidoxime (2-PAM), especially in case of sarin and tabun. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.cbi.2019.04.020DOI Listing

Differing calcification processes in cultured vascular smooth muscle cells and osteoblasts.

Exp Cell Res 2019 Apr 17. Epub 2019 Apr 17.

Department of Comparative Biomedical Sciences, Royal Veterinary College, London, UK. Electronic address:

Arterial medial calcification (AMC) is the deposition of calcium phosphate mineral, often as hydroxyapatite, in the medial layer of the arteries. AMC shares some similarities to skeletal mineralisation and has been associated with the transdifferentiation of vascular smooth muscle cells (VSMCs) towards an osteoblast-like phenotype. This study used primary mouse VSMCs and calvarial osteoblasts to directly compare the established and widely used in vitro models of AMC and bone formation. Read More

View Article

Download full-text PDF

Source
https://linkinghub.elsevier.com/retrieve/pii/S00144827193019
Publisher Site
http://dx.doi.org/10.1016/j.yexcr.2019.04.020DOI Listing
April 2019
1 Read

Recent advances in 3D printing: vascular network for tissue and organ regeneration.

Transl Res 2019 Apr 5. Epub 2019 Apr 5.

Department of Mechanical and Aerospace Engineering, The George Washington University, Washington, DC; Department of Electrical and Computer Engineering, The George Washington University, Washington, DC; Department of Biomedical Engineering, The George Washington University, Washington, DC; Department of Medicine, The George Washington University Medical Center, Washington, DC. Electronic address:

Over the past years, the fabrication of adequate vascular networks has remained the main challenge in engineering tissues due to technical difficulties, while the ultimate objective of tissue engineering is to create fully functional and sustainable organs and tissues to transplant in the human body. There have been a number of studies performed to overcome this limitation, and as a result, 3D printing has become an emerging technique to serve in a variety of applications in constructing vascular networks within tissues and organs. 3D printing incorporated technical approaches allow researchers to fabricate complex and systematic architecture of vascular networks and offer various selections for fabrication materials and printing techniques. Read More

View Article

Download full-text PDF

Source
https://linkinghub.elsevier.com/retrieve/pii/S19315244193007
Publisher Site
http://dx.doi.org/10.1016/j.trsl.2019.04.002DOI Listing
April 2019
1 Read

Urocortinergic system in the epididymis of the normal and cryptorchid dogs.

Reprod Domest Anim 2019 Apr 20. Epub 2019 Apr 20.

Department of Veterinary Medicine and Animal Productions, University of Naples "Federico II", Naples, Italy.

Cryptorchidism is associated with changes in the gonads and the spermatic duct system, which may cause infertility problems. Urocortin (UCN) is a corticotrophin-releasing hormone (CRH)-related peptide, which affects several functions of male genital organs. The aim of the present study was to investigate the expression of UCN and its receptors CRHR1 and CRHR2 using immunohistochemistry, western blotting, and real-time reverse transcription polymerase chain reaction in tissues collected from the epididymis of normal and cryptorchid dogs. Read More

View Article

Download full-text PDF

Source
https://onlinelibrary.wiley.com/doi/abs/10.1111/rda.13443
Publisher Site
http://dx.doi.org/10.1111/rda.13443DOI Listing
April 2019
1 Read

P300 Acetyltransferase is a Cytoplasm-to-Nucleus Shuttle for SMAD2/3 and TAZ Nuclear Transport in TGFβ-stimulated Hepatic Stellate Cells.

Hepatology 2019 Apr 20. Epub 2019 Apr 20.

Tumor Microenvironment and Metastasis, Hormel Institute, University of Minnesota, Austin, MN, USA.

Nuclear translocation of SMAD2/3, core transcription factors of TGFβ signaling, is critical for hepatic stellate cell (HSC) differentiation into metastasis-promoting myofibroblasts. SMAD2/3 have multiple coactivators, including WW domain-containing transcription regulator protein 1 (WWTR1 or TAZ) and p300 acetyltransferase. In the nucleus, TAZ binds to SMAD2/3 to prevent SMAD2/3 nuclear export. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/hep.30668DOI Listing

Prolactin Receptor Signaling Regulates a Pregnancy-Specific Transcriptional Program in Mouse Islets.

Endocrinology 2019 May;160(5):1150-1163

Division of Endocrinology, Department of Medicine, University of Alabama at Birmingham School of Medicine, Birmingham, Alabama.

Pancreatic β-cells undergo profound hyperplasia during pregnancy to maintain maternal euglycemia. Failure to reprogram β-cells into a more replicative state has been found to underlie susceptibility to gestational diabetes mellitus (GDM). We recently identified a requirement for prolactin receptor (PRLR) signaling in the metabolic adaptations to pregnancy, where β-cell-specific PRLR knockout (βPRLRKO) mice exhibit a metabolic phenotype consistent with GDM. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1210/en.2018-00991DOI Listing

BHap: a novel approach for bacterial haplotype reconstruction.

Bioinformatics 2019 Apr 20. Epub 2019 Apr 20.

Burnett School of Biomedical Science, College of Medicine, University of Central Florida, Orlando, FL, USA.

Motivation: The bacterial haplotype reconstruction is critical for selecting proper treatments for diseases caused by unknown haplotypes. Existing methods and tools do not work well on this task, because they are usually developed for viral instead of bacterial populations.

Results: In this study, we developed BHap, a novel algorithm based on fuzzy flow networks, for reconstructing bacterial haplotypes from next generation sequencing data. Read More

View Article

Download full-text PDF

Source
https://academic.oup.com/bioinformatics/advance-article/doi/
Publisher Site
http://dx.doi.org/10.1093/bioinformatics/btz280DOI Listing
April 2019
1 Read

Role of the immune system in vascular function and blood pressure control induced by fecal microbiota transplantation in rats.

Acta Physiol (Oxf) 2019 Apr 20:e13285. Epub 2019 Apr 20.

Department of Pharmacology, School of Pharmacy, University of Granada, Centro de Investigaciones Biomédicas, 18071, Granada, Spain.

Aim: High blood pressure (BP) is associated with gut microbiota dysbiosis. The aim of this study was to investigate whether changes in gut microbiota induced by exchanging the gut microbiota between spontaneously hypertensive rats (SHR) and normotensive Wistar-Kyoto (WKY) alter the gut-immune system interaction inducing changes in vascular function and BP.

Methods: Twenty-week-old recipient WKY and SHR were orally gavaged with donor fecal contents from WKY or SHR. Read More

View Article

Download full-text PDF

Source
https://onlinelibrary.wiley.com/doi/abs/10.1111/apha.13285
Publisher Site
http://dx.doi.org/10.1111/apha.13285DOI Listing
April 2019
1 Read

Extraciliary roles of the ciliopathy protein JBTS17 in mitosis and neurogenesis.

Ann Neurol 2019 Apr 20. Epub 2019 Apr 20.

Biomedical Science and Engineering Interdisciplinary Program, KAIST, Daejeon, Korea.

Objective: JBTS17 is a major gene mutated in ciliopathies such as Joubert syndrome and oral-facial-digital syndrome type VI. Most patients with loss of function mutations in JBTS17 exhibit cerebellar vermis hypoplasia and brain stem malformation. However, some patients with JBTS17 mutations show microcephaly and abnormal gyration. Read More

View Article

Download full-text PDF

Source
https://onlinelibrary.wiley.com/doi/abs/10.1002/ana.25491
Publisher Site
http://dx.doi.org/10.1002/ana.25491DOI Listing
April 2019
1 Read

The contribution of KSHV to mortality in hospitalized HIV-infected patients being investigated for tuberculosis in South Africa.

J Infect Dis 2019 Apr 20. Epub 2019 Apr 20.

Division of Medical Biochemistry and Structural Biology, Department of Integrative Biomedical Sciences, University of Cape Town, South Africa.

Background: Despite increasing numbers of human immunodeficiency virus (HIV)-infected South Africans receiving antiretroviral therapy (ART), tuberculosis remains the leading cause of mortality. Approximately 25% of patients treated for tuberculosis have microbiologically unconfirmed diagnoses. We assessed whether elevated Kaposi's sarcoma-associated Herpes Virus (KSHV) viral load (VL) contributes to mortality in hospitalized HIV-infected patients investigated for tuberculosis. Read More

View Article

Download full-text PDF

Source
https://academic.oup.com/jid/advance-article/doi/10.1093/inf
Publisher Site
http://dx.doi.org/10.1093/infdis/jiz180DOI Listing
April 2019
1 Read

Impact of sampling rate on statistical significance for single subject fMRI connectivity analysis.

Hum Brain Mapp 2019 Apr 19. Epub 2019 Apr 19.

Center for Neuroscience Imaging Research, Institute for Basic Science, Suwon, South Korea.

A typical time series in functional magnetic resonance imaging (fMRI) exhibits autocorrelation, that is, the samples of the time series are dependent. In addition, temporal filtering, one of the crucial steps in preprocessing of functional magnetic resonance images, induces its own autocorrelation. While performing connectivity analysis in fMRI, the impact of the autocorrelation is largely ignored. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/hbm.24600DOI Listing

Experiences of community mental health nurses in Japan as the basis of their nursing philosophies.

Authors:
Koji Tanaka

Perspect Psychiatr Care 2019 Apr 20. Epub 2019 Apr 20.

Department of Nursing, Kanazawa Medical University, Uchinada, Ishikawa, Japan.

Purpose: To investigate the experiences of Japanese community mental health nurses (CMHNs) that help form the basis of their nursing philosophies.

Design And Methods: This study uses the narrative research method. I conducted participant observation and narrative interviews with nine CMHNs in Japan. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/ppc.12386DOI Listing

Polyphenolic flavonoid (Myricetin) upregulated proteasomal degradation mechanisms: Eliminates neurodegenerative proteins aggregation.

J Cell Physiol 2019 Apr 19. Epub 2019 Apr 19.

Cellular and Molecular Neurobiology Unit, Indian Institute of Technology, Jodhpur, Rajasthan, India.

Major neurodegenerative disorders are characterized by the formation of misfolded proteins aggregates inside or outside the neuronal cells. Previous studies suggest that aberrant proteins aggregates play a critical role in protein homeostasis imbalance and failure of protein quality control (PQC) mechanism, leading to disease conditions. However, we still do not understand the precise mechanisms of PQC failure and cellular dysfunctions associated with neurodegenerative diseases caused by the accumulation of protein aggregates. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/jcp.28695DOI Listing

Reproducible pharmacokinetics.

J Pharmacokinet Pharmacodyn 2019 Apr 19. Epub 2019 Apr 19.

Departments of Medicine, Health Research and Policy, Biomedical Data Science, and Statistics, Stanford Prevention Research Center, Meta-Research Innovation Center at Stanford (METRICS), Stanford University, 1265 Welch Road, Medical School Office Building Room X306, Stanford, CA, 94305, USA.

Reproducibility is a highly desired feature of scientific investigation in general, and it has special connotations for research in pharmacokinetics, a vibrant field with over 500,000 publications to-date. It is important to be able to differentiate between genuine heterogeneity in pharmacokinetic parameters from heterogeneity that is due to errors and biases. This overview discusses efforts and opportunities to diminish the latter type of undesirable heterogeneity. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s10928-019-09621-yDOI Listing

ASO Author Reflections: Tending Towards a Personalized Medicine for Colorectal Carcinomatosis by Adding the RAS Mutation Status in the Workup for CRS and HIPEC.

Ann Surg Oncol 2019 Apr 19. Epub 2019 Apr 19.

Oncologic and Pancreatic Surgery Unit, University Hospital Reina Sofıa, Córdoba, Spain.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1245/s10434-019-07362-3DOI Listing

Frailty index is associated with increased risk of elevated BNP in an elderly population: the Rugao Longevity and Ageing Study.

Aging Clin Exp Res 2019 Apr 19. Epub 2019 Apr 19.

State Key Laboratory of Genetic Engineering and MOE Key Laboratory of Contemporary Anthropology, School of Life Science and Institutes of Biomedical Sciences, Fudan University, Shanghai, China.

Background And Aims: To explore whether frailty, defined by frailty index (FI), is associated with the risk of elevated B-type natriuretic peptide (BNP), a surrogate endpoint of cardiovascular events.

Methods: Data of 1382 community-dwelling elders who had no documented cardiovascular diseases aged 70-84 years from the ageing arm of the Rugao Longevity and Ageing Study was used. Traditional risk factor index (TI) was constructed using eight established cardiovascular-related risk factors. Read More

View Article

Download full-text PDF

Source
http://link.springer.com/10.1007/s40520-019-01189-4
Publisher Site
http://dx.doi.org/10.1007/s40520-019-01189-4DOI Listing
April 2019
1 Read

A Protocol for Sedation Free MRI and PET Imaging in Adults with Autism Spectrum Disorder.

J Autism Dev Disord 2019 Apr 19. Epub 2019 Apr 19.

Massachusetts General Hospital, Boston, MA, USA.

Imaging technologies such as positron emission tomography (PET) and magnetic resonance imaging (MRI) present unparalleled opportunities to investigate the neural basis of autism spectrum disorder (ASD). However, challenges such as deficits in social interaction, anxiety around new experiences, impaired language abilities, and hypersensitivity to sensory stimuli make participating in neuroimaging studies challenging for individuals with ASD. In this commentary, we describe the existent training protocols for preparing individuals with ASD for PET/MRI scans and our own experience developing a training protocol to facilitate the inclusion of low-functioning adults with ASD in PET-MRI studies. Read More

View Article

Download full-text PDF

Source
http://link.springer.com/10.1007/s10803-019-04010-3
Publisher Site
http://dx.doi.org/10.1007/s10803-019-04010-3DOI Listing
April 2019
1 Read

Inhibition of sodium-glucose cotransporter-2 preserves cardiac function during regional myocardial ischemia independent of alterations in myocardial substrate utilization.

Basic Res Cardiol 2019 Apr 19;114(3):25. Epub 2019 Apr 19.

Department of Cellular and Integrative Physiology, Indiana University School of Medicine, 635 Barnhill Drive, Indianapolis, IN, 46202, USA.

The goal of the present study was to evaluate the effects of SGLT2i on cardiac contractile function, substrate utilization, and efficiency before and during regional myocardial ischemia/reperfusion injury in normal, metabolically healthy swine. Lean swine received placebo or canagliflozin (300 mg PO) 24 h prior to and the morning of an invasive physiologic study protocol. Hemodynamic and cardiac function measurements were obtained at baseline, during a 30-min complete occlusion of the circumflex coronary artery, and during a 2-h reperfusion period. Read More

View Article

Download full-text PDF

Source
http://link.springer.com/10.1007/s00395-019-0733-2
Publisher Site
http://dx.doi.org/10.1007/s00395-019-0733-2DOI Listing
April 2019
1 Read

A high-affinity peptide substrate for G protein-coupled receptor kinase 2 (GRK2).

Amino Acids 2019 Apr 19. Epub 2019 Apr 19.

Division of Biopharmaceutics and Pharmacokinetics, National Cerebral and Cardiovascular Center Research Institute, 5-7-1 Fujishiro-dai, Suita, Osaka, 565-8565, Japan.

We synthesized a previously identified β-tubulin-derived G protein-coupled receptor kinase 2 (GKR2) peptide (GR-11-1; DEMEFTEAESNMN) and its amino-terminal extension (GR-11-1-N; GEGMDEMEFTEAESNMN) and carboxyl-terminal extension (GR-11-1-C; DEMEFTEAESNMNDLVSEYQ) peptides with the aim of finding a high-affinity peptide substrate for GRK2. GR-11-1-C showed high affinity for GRK2, but very low affinity for GKR5. Its specificity and sensitivity for GKR2 were greater than those of GR-11-1 and GR-11-1-N. Read More

View Article

Download full-text PDF

Source
http://link.springer.com/10.1007/s00726-019-02735-0
Publisher Site
http://dx.doi.org/10.1007/s00726-019-02735-0DOI Listing
April 2019
1 Read

The Function(s) of Sleep.

Handb Exp Pharmacol 2019 Apr 20. Epub 2019 Apr 20.

Department of Biology, Stanford University, Stanford, CA, USA.

Sleep is a highly conserved phenomenon in endotherms, and therefore it must serve at least one basic function across this wide range of species. What that function is remains one of the biggest mysteries in neurobiology. By using the word neurobiology, we do not mean to exclude possible non-neural functions of sleep, but it is difficult to imagine why the brain must be taken offline if the basic function of sleep did not involve the nervous system. Read More

View Article

Download full-text PDF

Source
http://link.springer.com/10.1007/164_2018_140
Publisher Site
http://dx.doi.org/10.1007/164_2018_140DOI Listing
April 2019
3 Reads
  • Page 1 of 11483