Biomed Opt Express 2017 Aug 31;8(8):3891-3902. Epub 2017 Jul 31.
Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia 20147, USA.
Adjusting the objective correction collar is a widely used approach to correct spherical aberrations (SA) in optical microscopy. In this work, we characterized and compared its performance with adaptive optics in the context of in vivo brain imaging with two-photon fluorescence microscopy. We found that the presence of sample tilt had a deleterious effect on the performance of SA-only correction. Read More