4,430 results match your criteria Biomedical chromatography : BMC[Journal]


Determination of GL-V9, a derivative of wogonin, in rat plasma by UPLC-MS/MS and its application to a pharmacokinetic study after oral and pulmonary administration.

Biomed Chromatogr 2019 Apr 16:e4556. Epub 2019 Apr 16.

Clinical Pharmacokinetics Laboratory, China Pharmaceutical University, Nanjing, Jiangsu Province, China.

GL-V9, a derivative of wogonin, shows much more potent anticancer properties than wogonin. In this study, a selective, sensitive and rapid ultra-high performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method was developed and validated for the determination of GL-V9 in rat plasma. Plasma samples were processed using methanol to precipitate protein. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/bmc.4556DOI Listing

Development of a simple method for simultaneous determination of tazarotene and betamethasone dipropionate and their metabolites using LC-MS method and its application to dermatopharmacokinetic study.

Biomed Chromatogr 2019 Apr 16:e4557. Epub 2019 Apr 16.

Institute of Dermatology, Chinese Academy of Medical Sciences and Peking Union Medical College, Nanjing, P.R. China.

In our study, the determination methodology of tazarotene and betamethasone dipropionate in human tissue engineering skin was established. The tazarotene gel, betamethasone dipropionate cream, or combination cream was administrated on the skin. Then the skin was taken off on 0. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/bmc.4557DOI Listing

Quantitative analysis of multi-components by single marker and quality evaluation of Venenum Bufonis from different geographical origins.

Biomed Chromatogr 2019 Apr 16:e4555. Epub 2019 Apr 16.

College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, China.

Bufadienolides are the main bioactive components of Venenum Bufonis (VB) and have been widely used to treat different types of human cancers for decades. The bufadienolides content in VB varies significantly from different geographical origins. In this work, a new strategy for the quality assessment of VB was developed through quantitative analysis of multi-components by single marker (QAMS). Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/bmc.4555DOI Listing

Analysis of phenolic acids and flavonoids in leaves of Lycium barbarum from different habitats by ultra-high performance liquid chromatography coupled with triple quadrupole tandem mass spectrometry.

Biomed Chromatogr 2019 Apr 15:e4552. Epub 2019 Apr 15.

Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, State Administration of Traditional Chinese Medicine Key Laboratory of Chinese Medicinal Resources Recycling Utilization, Nanjing University of Chinese Medicine, Nanjing, China.

The leaves of Lycium barbarum (LLB) have been utilized as crude drugs and functional tea for human health in China and Southeast Asia for thousands of years. To control its quality, a rapid and sensitive ultra-high performance liquid chromatography coupled with triple quadrupole tandem mass spectrometry (UPLC-TQ-MS) method was established and validated for the first time for simultaneous determination of 10 phenolic acids and flavonoids (including neochlorogenic acid, protocatechuic aldehyde, p-hydroxybenzoic acid, chlorogenic acid, cryptochlorogenic acid, caffeic acid, p-coumaric acid, ferulic acid, rutin and kaempferol-3-O-rutinoside) in LLB. The separation was performed on an ACQUITY UPLC C chromatographic column (100 mm × 2. Read More

View Article

Download full-text PDF

Source
https://onlinelibrary.wiley.com/doi/abs/10.1002/bmc.4552
Publisher Site
http://dx.doi.org/10.1002/bmc.4552DOI Listing
April 2019
1 Read

A validated RP-HPLC method for the evaluation of the influence of grapefruit juice on liver S9 activation of sacubitril.

Biomed Chromatogr 2019 Apr 15:e4553. Epub 2019 Apr 15.

Pharmaceutical Chemistry Department, Faculty of Pharmacy, Cairo University, Cairo, Egypt.

Grapefruit juice inhibit esterase enzyme. A possible interaction with ester prodrugs should be taken into consideration. In this study, influence of grapefruit juice on sacubitril (SAC) rat liver S9 activation by esterase enzyme was evaluated. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/bmc.4553DOI Listing

Highly-sensitive UHPLC-DAD method for simultaneous determination of two synergistically-acting antiepileptic drugs: Application to pharmaceutical tablets and human urine.

Biomed Chromatogr 2019 Apr 15:e4554. Epub 2019 Apr 15.

Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Assiut University, Assiut, Egypt.

A simple and highly sensitive Ultra High-performance Liquid Chromatographic -Diode array detection method (UHPLC-DAD) was developed and validated for the simultaneous estimation of levetiracetam (LEV) and lacosamide (LAC). It was clinically proven that the combination of LEV and LAC exhibits a synergistic effect against refractory seizures in mice, which was the motivation for the analysis of this binary mixture both in bulk and human urine samples. The binary mixture was resolved on a Hypersil BDS C analytical column, utilizing a mobile phase of 0. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/bmc.4554DOI Listing

Lipophilicity and bio-mimetic properties determination of phytoestrogens using ultra-high performance liquid chromatography.

Biomed Chromatogr 2019 Apr 13:e4551. Epub 2019 Apr 13.

Department of Pharmaceutical Analysis, University of Zagreb, Faculty of Pharmacy and Biochemistry, Zagreb, Croatia.

This paper presents lipophilicity and bio-mimetic properties determination of 15 phytoestrogens, namely: biochanin A, daidzein, formononetin, genistein, genistein-4,7-dimethylether, prunetin, 3,4,7-trihydroxyisoflavon, 4,6,7-trihydroxyisoflavon, 4,6,7-trimethoxyisoflavon, daidzin, genistin, ononin, sissotrin, coumestrol, coumestrol dimethylether. High performance liquid chromatography (HPLC) with fast gradient elution and Caco-2 cell line were used to determine physicochemical properties of selected phytoestrogens. Lipophilicity was determined on octadecyl-sylane (ODS) stationary phase using pH 2. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/bmc.4551DOI Listing

Development and validation of a sensitive liquid-chromatography tandem mass-spectrometry assay for mycophenolic acid and metabolites in HepaRG cell culture: characterization of metabolism interactions between p-cresol and mycophenolic acid.

Biomed Chromatogr 2019 Apr 8:e4549. Epub 2019 Apr 8.

Katz Group Centre for Pharmacy and Health Research, Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, Edmonton, Alberta, Canada.

Mycophenolic acid (MPA), a frequently used immunosuppressant, exhibits large inter-patient pharmacokinetic variability. This study 1) developed and validated a sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) assay for MPA and metabolites (MPA glucuronide [MPAG] and acyl-glucuronide [AcMPAG]) in the culture medium of HepaRG cells; and 2) characterized the metabolism interaction between mycophenolic acid and p-cresol (common uremic toxin) in this in vitro model as a potential mechanism of pharmacokinetic variability. Chromatographic separation was achieved with a C18 column (4. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/bmc.4549DOI Listing

Comparative pharmacokinetic study of four major bioactive components after oral administration of Zhi-Zi-Hou-Po decoction in normal and corticosterone-induced depressive rats.

Biomed Chromatogr 2019 Apr 4:e4542. Epub 2019 Apr 4.

Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, China.

A high selective and efficient LC-MS/MS method was developed to determine the plasma concentration of magnolol, hesperidin, neohesperidin and geniposide following oral administration of Zhi-Zi-Hou-Po decoction in normal and depressed rats. Plasma samples were pretreated by protein precipitation with methanol. Chromatographic separation was performed on an XTerra® MS C column using a gradient elution with a mobile phase composed of acetonitrile-0. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/bmc.4542DOI Listing
April 2019
3 Reads

Development and Validation of Bioanalytical Method for Quantification of Cycloserine in Human Plasma by Liquid Chromatography-Tandem Mass Spectrometry: Application to Pharmacokinetic Study.

Biomed Chromatogr 2019 Apr 4:e4548. Epub 2019 Apr 4.

Biotechnica Pharma, Hyderabad, India.

A selective, sensitive and high-throughput liquid chromatography-tandem mass spectrometry (LC-MS/MS) bioanalytical method has been developed for the estimation of cycloserine in human plasma, employing cytosine as the internal standard. The extraction of the analyte was facilitated by solid phase extraction using 100 μL of human plasma. The separation was carried on BDS Hypersil C (150 x 4. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/bmc.4548DOI Listing
April 2019
2 Reads

Corrigendum.

Authors:

Biomed Chromatogr 2019 05 27;33(5):e4504. Epub 2019 Feb 27.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/bmc.4504DOI Listing

Two Validated Chromatographic Determinations of an Anti-fungal Drug, its Toxic Impurities and Degradation Product: A comparative Study.

Biomed Chromatogr 2019 Apr 2:e4547. Epub 2019 Apr 2.

Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Beni-Suef University, Beni-Suef, Egypt.

Tolnaftate, a thionoester anti-fungal drug, was subjected to alkaline hydrolysis to produce methyl(m-tolyl)carbamic acid and β-naphthol (Tolnaftate impurity A). N-methyl-m-toluidine, Tolnaftate impurity D, was synthesized and structurally elucidated along with Tolnaftate alkaline degradation products using IR, H NMR and MS. Two stability-indicating HPTLC and RP-HPLC methods were developed and validated, for the first time, for determination of Tolnaftate, its alkaline degradation products and toxic impurities in presence of methyl paraben, as a preservative in Tinea Cure® cream. Read More

View Article

Download full-text PDF

Source
https://onlinelibrary.wiley.com/doi/abs/10.1002/bmc.4547
Publisher Site
http://dx.doi.org/10.1002/bmc.4547DOI Listing
April 2019
2 Reads

Development and validation of Liquid Chromatography - Electrospray - Tandem Mass Spectrometry method for determination of Flibanserin in human Plasma: Application to pharmacokinetic study on healthy female volunteers.

Biomed Chromatogr 2019 Apr 1:e4545. Epub 2019 Apr 1.

Zi-diligence Research Center, Al-Mokattam, Cairo, Egypt.

A novel rapid and highly sensitive Ultra Performance Liquid Chromatography-tandem mass spectrometry (UPLC-MS/MS) bioanalytical method was established for the analysis of Flibanserin in human plasma. Flibanserin d4 was used as internal standard (IS). Flibanserin and the internal standard (IS) was extracted from the plasma using precipitation technique with acetonitrile. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/bmc.4545DOI Listing
April 2019
1 Read

Simultaneous determination of major components of Huangqi-Honghua extract in rat plasma using LC-MS/MS and application to a pharmacokinetic study.

Biomed Chromatogr 2019 Apr 1:e4546. Epub 2019 Apr 1.

Department of Pharmaceutical Analysis, Pharmacy School, Shenyang Pharmaceutical University, Shenyang, China.

A sensitive and reliable LC-MS/MS method was developed and validated for simultaneous quantification of the major components of Huangqi-Honghua extact in rat plasma, including hydroxysafflor yellow A (HSYA), astragaloside IV (ASIV), calycosin-7-O-β-D-glucoside (CAG), calycosin, calycosin-3'-O-glucuronide (C-3'-G) and calycosin-3'-O-sulfate (C-3'-S). After extracted by protein precipitation with acetonitrile and methanol from plasma, the analytes were separated on a Hypersil BDS C18 column by gradient elution with acetonitrile and 5 mmol/L ammonium acetate. The detection was carried out on a triple quadrupole tandem mass spectrometer equipped with electrospray ionization source (ESI) switched between negative and positive modes. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/bmc.4546DOI Listing
April 2019
1 Read

Determination of terbinafine in human plasma using UPLC-MS/MS: Application to a bioequivalence study in healthy subjects.

Biomed Chromatogr 2019 Apr 1:e4543. Epub 2019 Apr 1.

Bioanalytical Department, Alkem Laboratories Ltd, Mumbai, India.

A high throughput and sensitive ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method has been developed for the determination of terbinafine in human plasma. The method employed liquid-liquid extraction of terbinafine and terbinafine-d7 (used as internal standard) from 100 μL human plasma with ethyl acetate: n-hexane (80: 20, v/v) solvent mixture. Chromatography was performed on BEH C18 (50 × 2. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/bmc.4543DOI Listing

Bioanalytical method for quantitative determination of mithramycin analogues in mouse plasma by HPLC-QTOF.

Biomed Chromatogr 2019 Mar 28:e4544. Epub 2019 Mar 28.

Department of Pharmaceutical Sciences, College of Pharmacy, University of Kentucky, Lexington, KY.

Mithramycin (MTM) has potent anticancer activity, but severe toxicities restrict its clinical use. Semi-synthetic approaches have yielded novel MTM analogues with potentially lower toxicity and similar efficacy. In an effort to transition these analogs into in vivo models, a bioanalytical method was developed for their quantification in mouse plasma. Read More

View Article

Download full-text PDF

Source
https://onlinelibrary.wiley.com/doi/abs/10.1002/bmc.4544
Publisher Site
http://dx.doi.org/10.1002/bmc.4544DOI Listing
March 2019
2 Reads

QSAR characterization of new synthesized hydantoins with antiproliferative activity.

Biomed Chromatogr 2019 Mar 30:e4539. Epub 2019 Mar 30.

Faculty of Sciences, Department of Chemistry, Biochemistry and Environmental Protection, University of Novi Sad, Trg Dositeja Obradovića 3, 2100, Novi Sad, Republic of Serbia.

Hydantois have been identified as constituents of a number of pharmacologically active molecules. In the present study, we have examined in vitro antiproliferative activity against human colon cancer cell lines HCT-116 of three series of 3-(4-substituted benzyl)-hydantoins with various substituent attached in position 5 of the hydantoin ring. Since the investigated compounds have recently been synthesized and show antiproliferative activity, a good understanding of the properties of the potential drug responsible for their pharmacokinetics is an important goal for their further development. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/bmc.4539DOI Listing

Safety evaluation of Semen Sojae Preparatum based on simultaneous LC-ESI-MS/MS quantification of Aflatoxin B , B , G , G and M.

Biomed Chromatogr 2019 Mar 30:e4541. Epub 2019 Mar 30.

Beijing Key Lab of TCM Collateral Disease Theory Research, School of Traditional Chinese Medicine, Capital Medical University, Beijing, China.

Semen Sojae Preparatum (SSP) is one of the most widely used traditional Chinese medicines, and is also a kind of functional food. However, contamination of aflatoxins may be present in the fermentation process of SSP. To evaluate its safety, an accurate and rapid LC-ESI-MS/MS analytical method was developed and validated for the simultaneous determination of AFB , AFB , AFG , AFG and AFM in SSP. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/bmc.4541DOI Listing

Simultaneous quantification of propofol, ketamine and rocuronium in just 10 μL plasma using liquid chromatography coupled with quadrupole mass spectrometry and its pilot application to a pharmacokinetic study in rats.

Biomed Chromatogr 2019 Mar 29:e4540. Epub 2019 Mar 29.

CBR- Center of Breath Research, Department of Anaesthesiology, Intensive Care and Pain Therapy, Saarland University Medical Center and Saarland University Faculty of Medicine, Homburg/Saar, Germany.

The combination of propofol, ketamine and rocuronium can be used for anesthesia of ventilated rats. However, reliable pharmacokinetic models of these drugs have yet to be developed in rats, and consequently optimal infusion strategies are also unknown. Development of pharmacokinetic models requires repeated measurements of drug concentrations. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/bmc.4540DOI Listing

Identification of conjugation positions of urinary glucuronidated vitamin D metabolites by LC/ESI-MS/MS after conversion to MS/MS-fragmentable derivatives.

Biomed Chromatogr 2019 Mar 25:e4538. Epub 2019 Mar 25.

Faculty of Pharmaceutical Sciences, Tokyo University of Science, Noda-shi, Chiba, Japan.

A liquid chromatography/electrospray ionization-tandem mass spectrometry-based method was developed for the identification of the conjugation positions of the monoglucuronides of 25-hydroxyvitamin D [25(OH)D ] and 24,25-dihydroxyvitamin D [24,25(OH) D ] in human urine. The method employed derivatization with 4-(4-dimethylaminophenyl)-1,2,4-triazoline-3,5-dione to convert the glucuronides into fragmentable derivatives, which provided useful product ions for identifying the conjugation positions during the MS/MS. The derivatization also enhanced the assay sensitivity and specificity for urine sample analysis. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/bmc.4538DOI Listing
March 2019
1 Read

A liquid chromatography-tandem mass spectrometry method to simultaneously determinate dichlorvos and phoxim in tobacco.

Biomed Chromatogr 2019 Mar 21:e4537. Epub 2019 Mar 21.

State Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Guiyang, China.

A simple pretreatment method with liquid chromatography-tandem mass spectrometry (LC-MS/MS) was developed and validated to simultaneously determine dichlorvos and phoxim in tobacco and soil matrices. Satisfactory linearity (R  ≥ 0.9991) of the method was obtained for both analytes. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/bmc.4537DOI Listing
March 2019
1 Read

Nontarget metabolomics profiling of neuromyelitis optica spectrum disorder.

Biomed Chromatogr 2019 Mar 19:e4533. Epub 2019 Mar 19.

Department of Neurology, Xiangya Hospital of Central South University, Changsha, Hunan, China.

To investigate the characteristic of neuromyelitis optica spectrum disorder (NMOSD) in plasma and cerebrospinal fluid, UHPLC-MS was used to identify metabolites by metabolite extraction and on-machine detection. Multivariate analysis methods were used to complete differential metabolite screening and metabolic pathway analysis. The content of eight substances, such as tryptophan and l-glutamic acid in the plasma of NMOSD patients, was higher than that of the healthy control group. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/bmc.4533DOI Listing
March 2019
6 Reads

A rapid and sensitive UHPLC-MS/MS method for the determination of celosin A in rat plasma with application to pharmacokinetic study.

Biomed Chromatogr 2019 Mar 18:e4535. Epub 2019 Mar 18.

Department of medical affairs, The Fifth People's Hospital of Shanghai, Shanghai, China.

Celosin A (CA), a natural compound isolated from Celosia argentea L., has been shown significant hepatoprotective effect on AHNP-induced liver injury. This study described a rapid and sensitive ultra-high-pressure liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) assay for determination of CA in rat plasma. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/bmc.4535DOI Listing

Metabolomics analysis of the therapeutic mechanism of Semen Descurainiae Oil on hyperlipidemia rats using H-NMR and LC-MS.

Biomed Chromatogr 2019 Mar 18:e4536. Epub 2019 Mar 18.

School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, Liaoning Province, China.

Semen descurainiae oil (SDO) is an important traditional Chinese medicine that was discovered to have the function of reducing blood lipid in recent years. Metabolomics approach of plasma, liver and kidney in rats were performed using H-NMR and LC-MS to illuminate the lower blood lipid concentration effect of SDO, and the niacin was considered as active control. The measure of total cholesterol (TC) and low-density lipoprotein cholesterol (LDL-C) in plasma showed that SDO treatment decreased significantly the content of TC and LDL-C. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/bmc.4536DOI Listing

A sensitive UPLC-MS/MS method for simultaneous determination of polyphenols in rat plasma: Application to a pharmacokinetic study of dispensing granules and standard decoction of Cinnamomum cassia twigs.

Biomed Chromatogr 2019 Mar 15:e4534. Epub 2019 Mar 15.

Jiangsu Key Laboratory of Chinese Medicine Processing, Nanjing University of Chinese Medicine, Nanjing, People's Republic of China.

This study established a rapid and reliable approach using liquid chromatography-tandem mass spectrometry for the simultaneous determination of cinnamic acid, vanillic acid and protocatechuic acid in rat plasma. This is the first report on a comparative pharmacokinetic study of dispensing granules and standard decoction of Cinnamomum cassia twigs in rats. After liquid-liquid extraction by ethyl acetate, the plasma samples were subjected to LC-MS/MS for multiple reaction monitoring. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/bmc.4534DOI Listing

Identification of metabolites of novel Anti-MRSA fluoroquinolone WCK 771 in mouse, rat, rabbit, dog, monkey and human urine using liquid chromatography tandem mass spectrometry.

Biomed Chromatogr 2019 Mar 12:e4532. Epub 2019 Mar 12.

Wockhardt Research Centre, D-4, MIDC, Aurangabad, India.

WCK 771 is an l-arginine salt of levonadifloxacin (LND) being developed in intravenous dosage form and has recently completed a phase III trial in India. The pharmacokinetics of WCK 771, a novel anti-MRSA fluoroquinolone, were examined in mice, rats, rabbits, dogs, monkeys and humans after systemic administration during pre-clinical and clinical investigations. Urine and serum were evaluated for identification of metabolites. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/bmc.4532DOI Listing

Simultaneous quantitation of 23 bioactive compounds in Tanreqing capsule by high-performance liquid chromatography electrospray ionization tandem mass spectrometry.

Biomed Chromatogr 2019 Mar 8:e4531. Epub 2019 Mar 8.

Department of Pharmacology, Shanghai University of Traditional Chinese Medicine, Shanghai, China.

Tanreqing capsule (TRQC) is a formulation frequently used in traditional Chinese medicine to treat pyrexia, cough, expectoration and pharyngalgia. Since the pharmacological action of traditional Chinese medicines is closely related to their complex and diverse constituents, understanding the exact composition of TRQC is important to elucidate its clinical effectiveness and mechanism of action as well as to establish quality control methods and resolve safety issues. Herein, we employed high-performance liquid chromatography coupled with electrospray ionization tandem mass spectrometry for the simultaneous quantitation of 23 bioactive compounds in five batches of TRQC; the analytes could be categorized into five types: organic acids (seven compounds), flavonoids (10 compounds), iridoids (two compounds), phenylethanoid glycosides (two compounds) and bile acids (two compounds). Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/bmc.4531DOI Listing
March 2019
1 Read
1.723 Impact Factor

Stability of mycophenolate mofetil in polypropylene 5% dextrose infusion bags and chemical compatibility associated with the use of the Equashield closed-system transfer device.

Biomed Chromatogr 2019 Mar 7:e4529. Epub 2019 Mar 7.

Institute for Health Research (INCLIVA), Clinical University Hospital of Valencia, Spain.

Stability studies are necessary in healthcare settings as they facilitate fast, cost-effective and efficient work related to batch manufacturing and availability of supplies. We studied the stability of 1-10 mg/mL mycophenolate mofetil (MMF) in polypropylene 5% dextrose infusion bags prepared from Cellcept and with a generic brand name (Micofenolato de Mofetilo Accord) at different storage temperatures. To ensure chemical compatibility during preparation, we also tested MMF sorption to the Equashield closed-system drug transfer device used in this step. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/bmc.4529DOI Listing
March 2019
3 Reads

Determination of D-limonene in mice plasma and tissues by a new GC-MS/MS method: Comparison of the pharmacokinetics, tissue distribution by oral and inhalation administration in mice.

Biomed Chromatogr 2019 Mar 7:e4530. Epub 2019 Mar 7.

Shanghai Institute for Food and Drug Control, Shanghai, China.

The aim of the present study was to develop a method based on gas chromatography-tandem mass spectrometry (GC-MS/MS) to determine and quantify the D-limonene (DL) in mice plasma and tissue samples. This new method was validated for the quantification of D-limonene with the linearity range between 1.0-1000. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/bmc.4530DOI Listing
March 2019
2 Reads
1.723 Impact Factor

Analytical methodologies for the determination of ticagrelor.

Biomed Chromatogr 2019 Mar 5:e4528. Epub 2019 Mar 5.

Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Medicine and Pharmacy Science and Technology from Târgu Mureș, Romania.

Ticagrelor, is an orally administered platelet aggregation inhibitor with a cyclopentyl-triazolopyrimidine structure; it is a selective reversible P2Y12 receptor antagonist, which prevents P2Y12-mediated and ADP-mediated platelet activation and aggregation. It is used to reduce the rate of cardiovascular death, myocardial infarction and stroke in patients with acute coronary syndrome or history of myocardial infarction. Several analytical methods have been published for the determination of ticagrelor in pharmaceuticals and biological materials by spectrophotometry, high-performance liquid chromatography with ultraviolet detection and liquid chromatography coupled with tandem mass spectrometry. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/bmc.4528DOI Listing
March 2019
5 Reads

UHPLC-MS/MS analysis of sphingosine 1-phosphate in joint cavity dialysate and hemodialysis solution of adjuvant arthritis rats: Application to geniposide pharmacodynamic study.

Biomed Chromatogr 2019 Mar 4:e4526. Epub 2019 Mar 4.

Anhui University of Chinese Medicine, Hefei, China.

Geniposide (GE) is an iridoid glycoside compound with anti-inflammatory effect. The potential of sphingosine 1-phosphate (S1P) as a plasma marker in human diseases was suggested recently in the literature, which demonstrated that, in patients with inflammatory diseases, plasma S1P was elevated. It follows that the obstructive coronary artery disease can be predicted with serum S1P. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/bmc.4526DOI Listing
March 2019
1 Read
1.723 Impact Factor

Validation of a HPLC/MS method for simultaneous quantification of clonidine, morphine and its metabolites in human plasma.

Biomed Chromatogr 2019 Mar 4:e4527. Epub 2019 Mar 4.

Department of Pharmaceutical Sciences, University of Kentucky, Lexington, KY, USA.

A high-performance liquid chromatography-tandem mass spectrometry method was developed and validated for the simultaneous quantification of morphine, morphine's major metabolites morphine-3-glucuronide and morphine-6-glucuronide, and clonidine, to support the pharmacokinetic analysis of an ongoing double-blinded randomized clinical trial that compares the use of morphine and clonidine in infants diagnosed with neonatal abstinence syndrome. Plasma samples were processed by solid-phase extraction and separated on an Inertsil ODS-3 (4 μm) column using an 0.1% formic acid in water-0. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/bmc.4527DOI Listing
March 2019
1 Read

Simultaneous determination of tandospirone (TDS) and its active metabolite, 1-[2-pyrimidyl]-piperazine (1-PP) in rat plasma by LC-MS/MS and its application to a pharmacokinetic study.

Biomed Chromatogr 2019 Mar 1:e4525. Epub 2019 Mar 1.

Department of Clinical Pharmacy and Pharmacy Administration, Key Laboratory of Drug Ministry of Education, West China School of Pharmacy, Sichuan University, Chengdu, Sichuan, China.

A rapid, sensitive, and selective liquid chromatography-tandem mass spectrometry method for the detection of tandospirone (TDS) and its active metabolite 1-[2-pyrimidyl]-piperazine (1-PP) in SD rat plasma is described. It was employed in a pharmacokinetic study. These analytes and the internal standards were extracted from plasma using protein precipitation with acetonitrile, then separated on a CAPCELL PAK ADME C column using a mobile phase of acetonitrile and 5 mM ammonium formate acidified with formic acid (0. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/bmc.4525DOI Listing
March 2019
1 Read

Neuropeptidomics: Comparison of parallel reaction monitoring and data-independent acquisition for the analysis of neuropeptides using high-resolution mass spectrometry.

Biomed Chromatogr 2019 Mar 1:e4523. Epub 2019 Mar 1.

Groupe de Recherche en Pharmacologie Animal du Québec (GREPAQ), Département de Biomédecine Vétérinaire, Faculté de Médecine Vétérinaire, Université de Montréal, Saint-Hyacinthe, Québec, Canada.

Targeted peptide quantitation by mass spectrometry is a rapidly emerging field. Traditionally it relied on the development and validation of multiple reaction monitoring assays that could comply with a high level of sensitivity, specificity, accuracy and reproducibility in complex biological samples. However, with the introduction of high-resolution mass spectrometers, other acquisition modes could provide more comprehensive datasets for identification and quantification but also for in-depth data mining. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/bmc.4523DOI Listing

Residue analysis of tebufenozide and indoxacarb in chicken muscle, milk, egg and aquatic animal products using liquid chromatography-tandem mass spectrometry.

Biomed Chromatogr 2019 Mar 1:e4522. Epub 2019 Mar 1.

Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Konkuk University, Seoul, Republic of Korea.

We developed an analytical method using liquid-liquid extraction (LLE) and liquid chromatography-tandem mass spectrometry (LC-MS/MS) to detect and quantify tebufenozide (TEB) and indoxacarb (IND) residues in animal and aquatic products (chicken muscle, milk, egg, eel, flatfish, and shrimp). The target compounds were extracted using 1% acetic acid (0.1% acetic acid for egg only) in acetonitrile and purified using n-hexane. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/bmc.4522DOI Listing

Validation of an LC-MS/MS method for simultaneous detection of diverse components of Qinxing Qingre Zhike Granule in rat plasma and its application to pharmacokinetic study after oral administration to rats.

Biomed Chromatogr 2019 Mar 1:e4524. Epub 2019 Mar 1.

Shanghai Hospital of Integrated Traditional Chinese and Western Medicine, Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, China.

A sensitive and validated method of liquid chromatography-tandem mass spectrometry (LC-MS/MS) was established to test the plasma concentrations of active ingredients in Qinxing Qingre Zhike Granule, namely geniposide, liquiritin, isoliquiritin, baicalin, wogonoside, baicalein, liquiritigenin, isoliquiritigenin and glycyrrhetinic acid. The analysis was performed on an Ultimate XB-C column at the flow rate of 0.4 mL min in a single run of 18 min. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/bmc.4524DOI Listing

Comparative pharmacokinetics of nine major bioactive components in normal and ulcerative colitis rats after oral administration of Lizhong decoction extracts by UPLC-TQ-MS/MS.

Biomed Chromatogr 2019 Feb 28:e4521. Epub 2019 Feb 28.

Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Nanjing University of Chinese Medicine, Nanjing, China.

Lizhong decoction (LZD), a classic formula, has been used to treat ulcerative colitis (UC) for thousands of years in clinical practice. However, the pharmacokinetic characteristics of its major bioactive components in rats under different physiological and pathological states are not clear. Thus, in this study, a rapid and sensitive analytical method, ultra-performance liquid chromatography coupled with mass spectrometry (UPLC-MS/MS) method, was developed and applied to simultaneously determine glycyrrhizic acid, liquiritin, isoliquiritin, glycyrrhizin, isoliquiritigenin, 6-gingerol, ginsenoside Rg1, ginsenoside Rb1 and ginsenoside Re in normal and UC rats after oral administration of LZD extract. Read More

View Article

Download full-text PDF

Source
http://doi.wiley.com/10.1002/bmc.4521
Publisher Site
http://dx.doi.org/10.1002/bmc.4521DOI Listing
February 2019
6 Reads

An LC-MS/MS method for the quantification of diclofenac sodium in dairy cow plasma and its application in pharmacokinetics studies.

Biomed Chromatogr 2019 Feb 28:e4520. Epub 2019 Feb 28.

Key Laboratory of New Animal Drug Project of Gansu Province, Key Laboratory of Veterinary Pharmaceutical Development of Ministry of Agriculture, Lanzhou Institute of Husbandry and Pharmaceutical Sciences of Chinese Academy of Agricultural Sciences, Lanzhou, China.

An LC-MS/MS method with internal standard tolfenamic acid for determining diclofenac sodium (DCF) in dairy cow plasma was developed and validated. Samples were processed with protein precipitation by cold formic acid-acetonitrile. Determination of DCF was performed using LC-ESI -MS/MS with the matrix-matched calibration curve. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/bmc.4520DOI Listing
February 2019
1 Read

Prediction of human intestinal absorption using micellar liquid chromatography with an aminopropyl stationary phase.

Biomed Chromatogr 2019 Feb 27:e4515. Epub 2019 Feb 27.

School of Engineering and Science, Medway Centre for Pharmaceutical Science, University of Greenwich, Chatham, Kent, UK.

The extent of human intestinal absorption (HIA) for a drug is considered to be an important pharmacokinetic parameter which must be determined for orally administered drugs. Traditional experimental methods relied upon animal testing and are renowned for being time consuming and expensive as well as being ethically unfavourable. As a result, the development of alternative methods to evaluate a drug's pharmacokinetics is crucial. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/bmc.4515DOI Listing
February 2019

A UPLC-MS/MS method for comparative pharmacokinetics study of morusin and morin in normal and diabetic rats.

Biomed Chromatogr 2019 Feb 27:e4516. Epub 2019 Feb 27.

Institute of Materia Medica, Shandong Academy of Medical Sciences, Jinan, Shandong, China.

The aim of this study was to establish and validate a rapid, selective and reliable ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) for simultaneous quantitations of morin and morusin, and to investigate their pharmacokinetics difference between normal and diabetic rats after oral administration. Plasma samples were pretreated via protein precipitation with acetonitrile. Genkwanin was used as internal standard (IS). Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/bmc.4516DOI Listing
February 2019

Chiral high-performance liquid and supercritical fluid chromatographic enantioseparations of limonene-based bicyclic aminoalcohols and aminodiols on polysaccharide-based chiral stationary phases.

Biomed Chromatogr 2019 May 13;33(5):e4517. Epub 2019 Mar 13.

Institute of Pharmaceutical Analysis, University of Szeged, Szeged, Hungary.

Enantioseparation of limonene-based bicyclic 1,3-aminoalcohols and 1,3,5- and 1,3,6-aminodiols was performed by normal-phase high-performance liquid chromatographic and supercritical fluid chromatographic (SFC) methods on polysaccharide-based chiral stationary phases. The effects of the composition of the mobile phase, the column temperature and the structures of the analytes and chiral selectors on retention and selectivity were investigated by normal-phase LC and SFC technique. Thermodynamic parameters derived from selectivity-temperature-dependence studies were found to be dependent on the chromatographic method applied, the nature of the chiral selector and the structural details of the analytes. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/bmc.4517DOI Listing

Simultaneous determination of eight bioactive components in rat plasma after oral administration of Yan-Ke-Ning-Tablet by liquid chromatography coupled with Q-Exactive Orbitrap tandem mass spectrometry.

Biomed Chromatogr 2019 Feb 26:e4519. Epub 2019 Feb 26.

Department of Pharmacy, Peking University People's Hospital, Beijing, China.

A rapid, sensitive and reliable quantitative method based on ultra-high performance liquid chromatography coupled with Q-Exactive Orbitrap tandem mass spectrometry was developed for simultaneous determination of berberine, berberrubine, palmatine, jatrorrhizine, columbamine, baicalin, baicalein and wogonin in rat plasma after oral administration with Yan-Ke-Ning-Tablet (YKNT). After precipitation with acetonitrile, the plasma samples were separated on a reverse-phase C column with 1 mm ammonium acetate containing 0.2% acetic acid-acetonitrile as mobile phase. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/bmc.4519DOI Listing
February 2019
1 Read

Development and validation of a rapid and sensitive UPLC-MS/MS assay for simultaneous quantification of paclitaxel and cyclopamine in mouse whole blood and tissue samples.

Biomed Chromatogr 2019 Feb 25:e4518. Epub 2019 Feb 25.

Department of Pharmacological and Pharmaceutical Sciences, University of Houston, Houston, TX, USA.

The prominent stromal compartment surrounds pancreatic ductal adenocarcinoma (PDAC) protects the tumor cells from chemo- or radio-therapy. We hypothesized that our nano formulation carried cyclopamine (CPA, stroma modulator) and paclitaxel (PTX, anti-tumor agent) could increase the permeation of PTX through the stromal compartment and improve the intratumoral delivery of PTX. In the present study a sensitive, reliable UPLC-MS/MS method was developed and validated to quantify PTX and CPA simultaneously in mouse whole blood, pancreas, liver and spleen samples. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/bmc.4518DOI Listing
February 2019

Pharmacokinetics and excretion study of bergenin and its phase II metabolite in rats by liquid chromatography tandem mass spectrometry.

Biomed Chromatogr 2019 Feb 21:e4513. Epub 2019 Feb 21.

Department of Pharmaceutical Analysis, School of Pharmacy Hebei Medical University, Shijiazhuang, China.

A sensitive and selective liquid chromatographic-tandem mass spectrometric (LC-MS/MS) method for the determination of bergenin and its phase II metabolite in rat plasma, bile and urine has been developed. Biological samples were pretreated with protein precipitation extraction procedure and enzymatic hydrolysis method was used for converting glucuronide metabolite to its free form bergenin. Detection and quantitation were performed by MS/MS using electrospray ionization and multiple reaction monitoring. Read More

View Article

Download full-text PDF

Source
http://doi.wiley.com/10.1002/bmc.4513
Publisher Site
http://dx.doi.org/10.1002/bmc.4513DOI Listing
February 2019
3 Reads

Stable isotope dilution assay for the accurate determination of tricaine in fish samples by HPLC-MS-MS.

Biomed Chromatogr 2019 May 6;33(5):e4512. Epub 2019 Mar 6.

Chinese Academy of Fishery Sciences, Beijing, P. R. China.

Tricaine methanesulfonate is one of most commonly used anesthetics in fish during blood sampling, artificial propagation and long-distance transportation. In this study, an accurate method for the quantitative determination of tricaine in fish samples by a stable isotope dilution assay coupled with high-performance liquid chromatography-triple quadrupole mass spectrometry was developed. Tricaine-D was synthesized and used as an isotopically labeled internal standard for the determination of tricaine. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/bmc.4512DOI Listing

Metabolism-based herb-drug interaction of Corydalis Bungeanae Herba with berberine in vitro and in vivo in rats.

Biomed Chromatogr 2019 Feb 20:e4514. Epub 2019 Feb 20.

Wuya College of Innovation, Shenyang Pharmaceutical University, Shenyang, Liaoning, P. R. China.

Corynoline (CRL) and berberine (BER) are the major bioactive components found in traditional Chinese medicines Corydalis Bungeanae Herba (Corydalis bungeanae) and Coptidis Rhizoma, respectively. The two herbs serve as anti-inflammatory agents and are generally applied to many prescriptions. The aims of the study were to evaluate herb-drug interaction of C. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/bmc.4514DOI Listing
February 2019
1.723 Impact Factor

Simultaneous determination of idelalisib and its metabolite GS-563117 in dog plasma by LC-MS/MS: Application to a pharmacokinetic study.

Biomed Chromatogr 2019 May 13;33(5):e4511. Epub 2019 Mar 13.

Department of Pharmacy, Jining No. 1 People's Hospital, Jining, China.

The purpose of this study was to develop and validate an LC-MS/MS method for simultaneous determination of idelalisib and GS-563117 in dog plasma. The analytes were extracted using ethyl acetate and then separated on a Waters Acquity UPLC BEH C column (50 × 2.1 mm, i. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/bmc.4511DOI Listing

LC-MS/MS method for simultaneous determination of ramelteon and its metabolite M-II in human plasma: Application to a clinical pharmacokinetic study in healthy Chinese volunteers.

Biomed Chromatogr 2019 May 15;33(5):e4510. Epub 2019 Mar 15.

Key Laboratory of Drug Quality Control and Pharmacovigilance (China Pharmaceutical University), Ministry of Education, Nanjing, China.

A sensitive liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) method was developed and validated for the simultaneous determination of ramelteon and its active metabolite M-II in human plasma. After extraction from 200 μL of plasma by protein precipitation, the analytes and internal standard (IS) diazepam were separated on a Hedera ODS-2 (5 μm, 150 × 2.1 mm) column with a mobile phase consisted of methanol-0. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/bmc.4510DOI Listing
May 2019
1 Read

The compatibility of six alkaloids in ermiao pill explored by a comparative pharmacokinetic and network pharmacological study.

Biomed Chromatogr 2019 May 13;33(5):e4509. Epub 2019 Mar 13.

Department of Pharmacy, Shanghai First Maternity and Infant Hospital, Tongji University School of Medicine, Shanghai, P.R. China.

Ermiao Pill (EMW), a traditional Chines medicine (TCM), composed of a two-herb pair, Phellodendri Cortex (PC) and Atractylodis Rhizome (AR), is used for the treatment of pelvic inflammatory disease and inflammatory-related diseases. However, the underlying mechanism is still unknown. Compatibility plays a crucial role in the complex drugs such as those used in traditional Chinese medicine. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/bmc.4509DOI Listing
May 2019
1 Read