4,754 results match your criteria Biomedical chromatography : BMC[Journal]


Development and validation of a LC-MS/MS method for the quantitative analysis of the adenosine A2a receptor antagonist NIR178 and its monohydroxy metabolite in human plasma: Application to clinical pharmacokinetics.

Biomed Chromatogr 2020 Jun 2:e4909. Epub 2020 Jun 2.

Pharmacokinetic Sciences-Safety & ADME Bioanalysis, Novartis Institutes for BioMedical Research, East Hanover, NJ, USA.

We report a selective LC-MS/MS method for the simultaneous quantitative determinations of the adenosine A2a receptor antagonist NIR178 (NIR178) and its major metabolite NJI765 in human plasma. Sample preparation steps involved protein precipitation, sample evaporation and reconstitution using a plasma sample volume of 0.1 mL plasma. Read More

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http://dx.doi.org/10.1002/bmc.4909DOI Listing

A strategy for qualitative and quantitative profiling of Angelicae Pubescentis Radix and detection of its analgesic and anti-inflammatory components by spectrum-effect relationship and multivariate statistical analysis.

Biomed Chromatogr 2020 May 30:e4910. Epub 2020 May 30.

Key Laboratory of Chinese Materia Medica, Heilongjiang University of Chinese Medicine, Ministry of Education, Harbin, China.

This study established a spectrum-effect relationship method for screening and quantifying the analgesic and anti-inflammatory active ingredients in Angelicae Pubescentis Radix (AP) by ultra-high-performance liquid chromatography-quadrupole mass spectrometry detector analysis (UPLC-QDA). First, the fingerprint of AP was established to determine the common peaks. Next, six batches of AP samples, with significant differences, were selected for evaluation of pharmacological activity. Read More

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http://dx.doi.org/10.1002/bmc.4910DOI Listing

A simplified and reliable LC-MS/MS method for determination of ulipristal acetate in human plasma and its application to a pharmacokinetic study in healthy Chinese subjects.

Biomed Chromatogr 2020 May 27:e4908. Epub 2020 May 27.

Department of Pharmaceutical Analysis, China Pharmaceutical University, Nanjing, China.

In this study, a simplified, sensitive and reliable liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was established and validated for the quantification of ulipristal acetate (UPA) in human plasma and for the investigation of pharmacokinetic profile of UPA following single oral administration of ella® (UPA 30 mg tablet) in healthy Chinese volunteers. Plasma samples were analyzed after being processed by protein precipitation with methanol. Chromatographic separation was performed on a Kinetex® EVO C column (2. Read More

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http://dx.doi.org/10.1002/bmc.4908DOI Listing

Capillary electrophoresis and ultra-high performance liquid chromatography methods in clinical monitoring of creatinine in human urine: a comparative study.

Biomed Chromatogr 2020 May 27:e4907. Epub 2020 May 27.

Department of Pharmaceutical Analysis and Nuclear Pharmacy, Faculty of Pharmacy, Comenius University in Bratislava, Bratislava, Slovak Republic.

Creatinine is an important diagnostic marker and is also used as a standardization tool for the quantitative evaluation of exogenous/endogenous substances in urine. An aim of this study was to evaluate and compare three analytical approaches, based on hyphenations of different separation (2D capillary isotachophoresis, CITP-CITP; capillary zone electrophoresis, CZE; ultra-high performance liquid chromatography, UHPLC) and detection (conductivity, CD; ultraviolet, UV; tandem mass spectrometry, MS/MS) techniques, for their ability to provide reliable clinical data along with their suitability for the routine clinical use (cost, simplicity, sample throughput). The developed UHPLC-MS/MS, CITP-CITP-CD, and CZE-UV methods were characterized by favorable performance parameters, such as linearity (r˃0. Read More

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http://dx.doi.org/10.1002/bmc.4907DOI Listing

Method development and validation for simultaneous determination of Ebastine and its active metabolite Carebastine in human plasma by LC-MS/MS and its application to clinical pharmacokinetic study in healthy Chinese volunteers.

Biomed Chromatogr 2020 May 25:e4904. Epub 2020 May 25.

Department of Pharmaceutical Analysi, China Pharmaceutical University, Nanjing, China.

Simple LC-MS/MS method to determine Ebastine and Carebastine (active metabolite) in human plasma was developed and validated. Analytes and internal standards precipitated by protein precipitation and separated on SynergiTM Hydro-RP 80A column (4 μm, 50 mm × 2.0 mm, Phenomenex) by gradient elution with mobile phase A: 0. Read More

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http://dx.doi.org/10.1002/bmc.4904DOI Listing

LC-APCI-MS/MS assay for quantitation of ethyl esters of eicosapentaenoic acid and docosahexaenoic acid in human plasma and its application in a pharmacokinetic study.

Biomed Chromatogr 2020 May 25:e4905. Epub 2020 May 25.

Department of Pharmaceutical Analysis, China Pharmaceutical University, Nanjing, China.

A simple and specific LC-MS/MS method was developed and validated for the determination of ethyl ester of eicosapentaenoic acid (EPAEE) and ethyl ester of docosahexaenoic acid (DHAEE). After de-protein with acetonitrile, the plasma samples were separated on a C18 column using a gradient elution system consisted of methanol and 1.0 mM ammonium acetate in water. Read More

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http://dx.doi.org/10.1002/bmc.4905DOI Listing

Physico-chemical evaluation of new tetrahydroacridine and iodobeznoic acid hybrids as the next step in the design of potential drugs in AD.

Biomed Chromatogr 2020 May 25:e4906. Epub 2020 May 25.

Medical University, Department of Pharmaceutical Chemistry and Drug Analyses, Lodz, Poland.

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http://dx.doi.org/10.1002/bmc.4906DOI Listing

Method Development and Validation of LC-MS/MS-Based Assay for the Simultaneous Quantitation of Trastuzumab and Pertuzumab in Cynomolgus Monkey Serum and its Application in Pharmacokinetic Study.

Biomed Chromatogr 2020 May 19:e4903. Epub 2020 May 19.

School of Chemical Engineering, Tianjin Universit, Tianjin, China.

We present a simple and robust LC-MS/MS assay for the simultaneous quantitation of an antibody cocktail of Trastuzumab and Pertuzumab in monkey serum. The LC-MS/MS method saved costs, decreased the analysis time and reduced quantitative times relative to the traditional ligand-binding assays. The serum samples were digested with trypsin at 50 °C for 60 min after methanol precipitation, ammonium bicarbonate denaturation, dithiothreitol reduction, and iodoacetamide alkylation. Read More

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http://dx.doi.org/10.1002/bmc.4903DOI Listing

A comparative analysis of raw and processed Radix Paeoniae Alba using high performance liquid chromatography, chemometrics and antioxidant activity.

Biomed Chromatogr 2020 May 19:e4901. Epub 2020 May 19.

Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, China.

Radix Paeoniae Alba (RPA) and its processed products are commonly used in traditional Chinese medicine, but the chaos phenomenon with different processed products often appears. In this study, we used multiple chemometric methods to analyze raw and six different processed products of RPA based on high performance liquid chromatography fingerprinting. Heat map analysis was used to assess the changes in chemical composition. Read More

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http://dx.doi.org/10.1002/bmc.4901DOI Listing

Metabolomic characterization of semen from asthenozoospermic patients using ultra-high-performance liquid chromatography-tandem quadrupole time-of-flight mass spectrometry.

Biomed Chromatogr 2020 May 19:e4897. Epub 2020 May 19.

Zhejiang Provincial Key Laboratory of Orthopedics, Department of Orthopedics, the Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, China.

Asthenozoospermia is a common factor of male infertility, and its pathogenesis is still unclear. The purpose of this study was to investigate the differential seminal plasma metabolic pattern in asthenozoospermic men and to identify potential biomarkers in relation to spermatogenic dysfunction using sensitive ultra-high-performance liquid chromatography-tandem quadruple time-of-flight mass spectrometry (UHPLC-Q-TOF/MS). The samples of seminal plasma from patients with asthenozoospermia (n = 20) and healthy controls (n = 20) were checked and differentiated by UHPLC-Q-TOF/MS. Read More

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http://dx.doi.org/10.1002/bmc.4897DOI Listing

A rapid HPLC-MS/MS method for determining busulfan in hemolysis samples from children with hematopoietic stem cell transplantation.

Biomed Chromatogr 2020 May 19:e4898. Epub 2020 May 19.

Clinical Research Center, Beijing Children's Hospital, Capital Medical University, Beijing, China.

A rapid and sensitive method for the quantitative detection of busulfan in children's hemolysis samples by HPLC-MS/MS was established. In this study, the sample preparation procedure involved a one-step protein precipitation with acetonitrile solution, and the HPLC-MS/MS method used Hypersil GOLD C18. The mobile phase consisted of 10mM ammonium acetate solution (containing 0. Read More

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http://dx.doi.org/10.1002/bmc.4898DOI Listing

Development and validation of a specific and sensitive LC-MS/MS method for determination of tetrodotoxin in human urine and its pharmacokinetic study.

Biomed Chromatogr 2020 May 19:e4900. Epub 2020 May 19.

Third Institute of Oceanography, Ministry of Natural Resource, Xiamen, Fujian, P.R. China.

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http://dx.doi.org/10.1002/bmc.4900DOI Listing

Rapid and cost-effective LC-MS/MS method for determination of hydroxycitric acid in plasma: Application in the determination of pharmacokinetics in commercial Garcinia preparations.

Biomed Chromatogr 2020 May 19:e4902. Epub 2020 May 19.

Department of Pharmacy, Birla Institute of Technology and Science (BITS) Pilani, Rajasthan, India.

Garcinia cambogia is one of the most commonly used anti-obesity dietary supplements and hydroxycitric acid (HCA) is a major constituent in the commercial preparations of Garcinia. High doses of HCA are often consumed without much awaremess of its pharmacokinetic and toxicokinetic parameters, and hence a full understanding of HCA effects is lacking. The first step in understanding of these parameters is the availability of a reliable bioanalytical method. Read More

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http://dx.doi.org/10.1002/bmc.4902DOI Listing

Validation of a sensitive and specific LC-MS/MS Method and application to evaluate the systemic exposure and bioavailability of glycopyrrolate via different drug delivery approaches/devices.

Biomed Chromatogr 2020 May 19:e4899. Epub 2020 May 19.

Sunovion Pharmaceuticals, Inc., Marlborough, MA, USA.

A specific and sensitive LC-MS/MS method using d -glycopyrrolate as the internal standard (IS) was developed for the quantitative determination of glycopyrrolate (GLY) in human plasma over a concentration range of 4.00-2000 pg/mL. GLY and IS were extracted by solid-phase extraction cartridge, then eluted with 0. Read More

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http://dx.doi.org/10.1002/bmc.4899DOI Listing

Assessment of Synthetic Cannabinoid FUB-AMB and its Ester Hydrolysis Metabolite in Human Liver Microsomes and Human Blood Samples by UHPLC-MS/MS.

Biomed Chromatogr 2020 May 15:e4884. Epub 2020 May 15.

Temple University Department of Chemistry, Philadelphia, PA, USA.

FUB-AMB, an indazole carboxamide synthetic cannabinoid recreational drug, was one of the compounds most frequently reported to governmental agencies worldwide between 2016-2019. It has been implicated in intoxications and fatalities, posing a risk to public health. In the current study, FUB-AMB was incubated with human liver microsomes (HLM) to assess its metabolic fate and stability and to determine if its major ester hydrolysis metabolite (M1) was present in twelve authentic forensic human blood samples from DUID and postmortem investigations via UHPLC-MS/MS. Read More

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http://dx.doi.org/10.1002/bmc.4884DOI Listing

Achiral and chiral analysis of duloxetine by chromatographic and electrophoretic methods, a review on the separation methodologies.

Biomed Chromatogr 2020 May 12:e4883. Epub 2020 May 12.

Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Medicine, Pharmacy Science and Technology of Târgu Mureş, Romania.

Duloxetine (DLX) is a widely used antidepressant drug belonging to the class of selective serotonin and norepinephrine reuptake inhibitors (SNRIs); its efficacy has been demonstrated in the treatment of not only major depressive disorders but also diabetic neuropathic pain, generalized anxiety disorder, fibromyalgia or stress urinary incontinence. It is a chiral substance and is used in therapy in the form of the enantiopure S-DLX which is twice as active as R-DLX. Several methods have been published for the achiral and chiral determination of DLX in pharmaceuticals, biological materials and environmental samples, the majority using liquid chromatography (LC) and capillary electrophoresis (CE) coupled with different detection techniques (UV detection, fluorescence, mass spectrometry). Read More

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http://dx.doi.org/10.1002/bmc.4883DOI Listing

Rapid identification of chemical composition and metabolites of Pingxiao Capsule in vivo using molecular networking and untargeted data-dependent tandem mass spectrometry.

Biomed Chromatogr 2020 May 12:e4882. Epub 2020 May 12.

Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, P.R. China.

Pingxiao capsule (PXC) is a herbal medicine used for adjuvant therapy in breast cancer. However, the constituents and absorbed components of the formula and their related metabolites have not been elucidated to date. PXC is a typical traditional Chinese medicine (TCM) formula consisting of Strychnos nux-vomica L. Read More

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http://dx.doi.org/10.1002/bmc.4882DOI Listing

Spleen and thymus metabolomics strategy to explore the immunoregulatory mechanism of total withanolides from the leaves of Datura metel L. on imiquimod-induced psoriatic skin dermatitis in mice.

Biomed Chromatogr 2020 May 12:e4881. Epub 2020 May 12.

Key Laboratory of Chinese Materia Medica, Ministry of Education of Heilongjiang University of Chinese Medicine, Harbin, People's Republic of China.

Our previous work demonstrated that total withanolides of Datura metel L. leaves (TWD) exhibited excellent therapeutic effects on psoriasis. However, current knowledge of its mechanisms is not deep enough. Read More

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http://dx.doi.org/10.1002/bmc.4881DOI Listing

A new HPLC-DAD method for contemporary quantification of 10 antibiotics for therapeutic drug monitoring of critically ill pediatric patients.

Biomed Chromatogr 2020 May 12:e4880. Epub 2020 May 12.

Department of Pediatric Medicine, Laboratory of Metabolic Biochemistry Unit, Bambino Gesù Children's Hospital, IRCCS, Rome, Italy.

The common practice of Therapeutic Drug Monitoring (TDM) involves the quantification of drug plasma concentrations at a specific time in a dosing window. Although TDM for antibiotics is not considered mandatory, it may represent a valid tool for clinicians in order to limit antibiotic resistance and avoid therapeutic failures. The aim of our study was to develop and validate High Performance Liquid Chromatography associated to Diode Array Detection (HPLC-DAD) method for contemporary quantification of ten antibiotics in plasma. Read More

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http://dx.doi.org/10.1002/bmc.4880DOI Listing

Uncovering the anticancer mechanism of petroleum extracts of Farfarae Flos against Lewis lung cancer by metabolomics and network pharmacology analysis.

Biomed Chromatogr 2020 May 9:e4878. Epub 2020 May 9.

Modern Research Center for Traditional Chinese Medicine of Shanxi University, Taiyuan, Shanxi, People's Republic of China.

Lung cancer shows the highest incidence rate in the world. Thus, it has increasingly become an important issue to find the therapeutic drugs to treat lung cancer. Farfarae Flos (FF) has been used in the traditional Chinese medicines (TCMs) in the treatment for the pulmonary diseases such as cough, bronchitis and asthmatic disorders. Read More

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http://dx.doi.org/10.1002/bmc.4878DOI Listing

Dimethyldioctadecylanimonium bentonite immobilized magnetic chitosan nanoparticles as an efficient adsorbent for vortex-assisted magnetic dispersive micro-solid-phase extraction of celecoxib from human breast milk, plasma and urine samples.

Biomed Chromatogr 2020 May 9:e4877. Epub 2020 May 9.

Department of Analytical Chemistry, Faculty of Chemistry, University of Mazandaran, Babolsar, Iran.

A polymer/layered silicate composite based on dimethyldioctadecylanimonium bentonite/chitosan magnetic nanoparticles was synthesized and characterized by field emission transmission electron microscopy, X-ray diffraction and Fourier transform infrared spectrometry. The prepared nanocomposite was used to isolate and preconcentrate celecoxib from human breast milk, urine and plasma samples. In this method, dimethyldioctadecylanimonium bentonite increases the accessibility of binding sites and adsorption capacity by high microporosity and large surface area, that has been realized for the first time in a magnetic chitosan nanoparticle support. Read More

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http://dx.doi.org/10.1002/bmc.4877DOI Listing

Enantioselective LC-ESI-MS/MS method for quantitation of (-)-lumefantrine and (+)-lumefantrine in mice plasma and application to a pharmacokinetic study.

Biomed Chromatogr 2020 May 9:e4879. Epub 2020 May 9.

Drug Metabolism and Pharmacokinetics, Jubilant Biosys Ltd, Bangalore, India.

We developed and validated a simple, sensitive, selective and reliable LC-MS/MS-ESI method for direct quantitation of lumefantrine (LFN) enantiomers [(-)-LFN and (+)-LFN] in mice plasma as per regulatory guideline. LFN enantiomers and carbamazepine (internal standard; IS) were extracted from mice plasma using Strata X SPE (solid phase extraction) cartridges. Good resolution between enantiomers was achieved on a Chiralpak IA-3 column using an isocratic mobile phase (0. Read More

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http://dx.doi.org/10.1002/bmc.4879DOI Listing

Serum metabolomic profiling in patients with Alzheimer Disease and Amnestic Mild Cognitive Impairment by GC/MS.

Biomed Chromatogr 2020 May 8:e4875. Epub 2020 May 8.

Department of Neurology, Qilu Hospital of Shandong University, Jinan, Shandong Province, China.

Objectives: The aim of this study is to characterize the serum metabolic profiles of patients with Alzheimer Disease (AD) and Amnestic Mild Cognitive Impairment (AMCI) using metabolomics based on GC/MS.

Methods: Serum samples were collected from patients with AD (n=30), AMCI (n=32), and normal healthy controls (NOR, n=40). Metabolite profiles were performed with gas chromatography-mass spectrometry (GC/MS) in conjunction with multivariate statistical analysis, and possible biomarker metabolites were identified. Read More

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http://dx.doi.org/10.1002/bmc.4875DOI Listing

Determination of diuretics in human urine using HPLC coupled with magnetic solid-phase extraction based on a metal-organic framework.

Biomed Chromatogr 2020 May 8:e4876. Epub 2020 May 8.

College of Physical Education and Training, Shanghai University of Sport, Shanghai, P. R. China.

Magnetic solid-phase extraction (MSPE) employing a metal-organic framework (Fe O @UiO-66-OH) combined with high-performance liquid chromatography was developed for the determination of trace diuretics in urine. The structure and properties of Fe O @UiO-66-OH were investigated using X-ray diffraction, infrared spectroscopy, scanning electron microscopy and vibrating sample magnetometry. Magnetic solid-phase extraction conditions, such as adsorbent amount and solution pH, were optimized using response surface methodology. Read More

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http://dx.doi.org/10.1002/bmc.4876DOI Listing

Enantioselective analysis of venlafaxine and its active metabolites: A review on the separation methodologies.

Biomed Chromatogr 2020 May 5:e4874. Epub 2020 May 5.

Department of Cell Biology and Microbiology, Faculty of Pharmacy, University of Medicine, Pharmacy, Science and Technology of Târgu Mureș, Târgu Mureş, Romania.

Venlafaxine (VFX) is a serotonin and norepinephrine reuptake inhibitor chiral drug used in therapy as an antidepressant in the form of a racemate consisting of R- and S-VFX. The two enantiomers of VFX exhibit different pharmacological activities: R-VFX inhibits both norepinephrine and serotonin synaptic reuptake, whereas S-VFX inhibits only the serotonin one. R- and S-VFX are metabolized in the liver to the respective R- and S-O-desmethylvenlafaxine (ODVFX), R- and S-N-desmethylvenlafaxine (NDVFX), and R- and S-N,O-didesmethylvenlafaxine (NODVFX). Read More

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http://dx.doi.org/10.1002/bmc.4874DOI Listing

A Liquid chromatography coupled to quadrupole/time-of-flight mass spectrometer (LC-QTOF-MS) method for identification analysis of saponins from Quillaja saponaria bark extracts in foot-and-mouth disease vaccines: development, validation and applicability.

Biomed Chromatogr 2020 May 5:e4873. Epub 2020 May 5.

Laboratório Federal de Defesa Agropecuária do Rio Grande do Sul - LFDA/RS, Porto Alegre, RS, Brazil.

Saponins from Quillaja saponaria have been commonly used as immunomodulatory adjuvants in foot-and-mouth disease vaccines (FMDV). However, due to the lack of consensus over the possible exacerbation of local inflammatory responses in cattle and its economic impacts, their use has been discouraged by Brazilian authorities. A qualitative method intended to determinate the presence of saponins from Q. Read More

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http://dx.doi.org/10.1002/bmc.4873DOI Listing

The effect of pressure on the characteristics of supercritical carbon dioxide extracts from Calamintha nepeta subsp. nepeta.

Biomed Chromatogr 2020 May 3:e4871. Epub 2020 May 3.

Research Unit 13ES63, Applied Chemistry and Environment, Faculty of Sciences of Monastir, University of Monastir, Monastir-5000, Tunisia.

Chemists and industrialists are continuously attempting to develop greener and more environmentally benign chemical processes to extract essential oils and bioactive metabolites of high purity, finding various applications in cosmetics, detergents, nutraceuticals and pharmaceuticals. An increase preferenced for natural products over synthetic ones has made supercritical fluid technology a primary alternative for the generation of high-value bioactive ingredients. This effective technique requires only moderate temperatures, eliminates clean-up steps and avoids the use of harmful organic solvents. Read More

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http://dx.doi.org/10.1002/bmc.4871DOI Listing

Development of a sensitive and rapid UHPLC-MS/MS method for simultaneous quantification of nine compounds in rat plasma and application in a comparative pharmacokinetic study after oral administration of Xuefu Zhuyu Decoction and nimodipine.

Biomed Chromatogr 2020 May 1:e4872. Epub 2020 May 1.

Guangzhou Analytical Application Center, Shimadzu Corporation, Guangzhou, P.R. China.

Xuefu Zhuyu Decoction (XFZYD) is a traditional Chinese medicine prescription used for the clinical treatment of traumatic brain injury (TBI). The purpose of this work was to develop a sensitive and rapid UHPLC-MS/MS method to simultaneously study the pharmacokinetics of nimodipine and eight components of XFZYD, namely, amygdalin, hydroxysafflor yellow A, rutin, liquiritin, narirutin, naringin, neohesperidin and saikosaponin A, in rats with and without TBI. MRM was highly selective in the detection of 9 analytes and the internal standard without obvious interference. Read More

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http://dx.doi.org/10.1002/bmc.4872DOI Listing

Pharmacokinetic study of tanshinol and ligustrazine in rat plasma after intravenous administration of tanshinol and Danshen Chuanxiongqin Injection.

Biomed Chromatogr 2020 Apr 28:e4869. Epub 2020 Apr 28.

Department of Pharmacy, The First Hospital of Jiaxing, Affiliated Hospital of Jiaxing University, Jiaxing, Zhejiang, China.

To investigate the effect of ligustrazine on the pharmacokinetic profile of tanshinol after intravenous administration in rats, a sensitive liquid chromatography tandem mass spectrometry method was developed and validated for quantitative determination of tanshinol and ligustrazine in rat plasma. After prepared by protein precipitation, the analytes were separated on a Waters Acquity HSS T3 column (100 × 2.1 mm, 1. Read More

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http://dx.doi.org/10.1002/bmc.4869DOI Listing
April 2020
1.723 Impact Factor

Simultaneous determination of TUG-891 and its metabolites in rat plasma using LC-HRMS with application to preclinical pharmacokinetic study.

Biomed Chromatogr 2020 Apr 28:e4870. Epub 2020 Apr 28.

Department of Clinical Pharmacy, Translation Medicine Institute, The First People's Hospital of Chenzhou, University of South China, Chenzhou, Hunan Province, China.

In this study, a simple and reliable LC-MS/MS method was first proposed for the simultaneous determination of TUG-891 and its metabolites TUG-891-alcohol, TUG-891-aldehyde, and TUG-891-acid in rat plasma. The analytes and fasiglifam (internal standard) were extracted from plasma samples with acetonitrile and separated using an Acquity BEH C column (1.7 μm, 2. Read More

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http://dx.doi.org/10.1002/bmc.4870DOI Listing

Comparative in vivo pharmacokinetics study of affeic acid, isoferulic acid and ferulic acid in crude and three different prepared Cimicifuga foetida L.

Biomed Chromatogr 2020 Apr 26:e4868. Epub 2020 Apr 26.

Jiangsu Key Laboratory of Chinese Medicine Processing, Engineering Center of State Ministry of Education for Standardization of Chinese Medicine Processing, Nanjing University of Chinese Medicine, Nanjing, China.

Our study investigated the differences in pharmacokinetics of three major components of crude Cimicifuga foetida L.and its fried product, honey- and liquor-prepared products. A rapid and sensitive ultra-high performance liquid chromatography with tandem mass spectrometry approach was established for determing caffeic acid, isoferulic acid and ferulic acid in rat plasma. Read More

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http://dx.doi.org/10.1002/bmc.4868DOI Listing
April 2020
1.723 Impact Factor

UPLC-MS/MS simultaneously determination of seven active ingredients of Yaobitong capsule in rat plasma and its integrated pharmacokinetic application.

Biomed Chromatogr 2020 Apr 24:e4866. Epub 2020 Apr 24.

School of Pharmacy, Shenyang Pharmaceutical University, Benxi, China.

A reliable and sensitive ultra performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method was firstly established and validated for simultaneously determine seven active ingredients of Yaobitong capsule in rat plasma, including ginsenoside Rg1, ginsenoside Rb1, osthole, tetrahydropalmatine, paeoniflorin, albiflorin and ferulic acid. And this method was further applied for the integrated pharmacokinetic study of Yaobitong capsule in rats after oral administration. Plasma samples (100 μL) were precipitated with 300 μL methanol using carbamazepine as internal standard (IS). Read More

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http://dx.doi.org/10.1002/bmc.4866DOI Listing

Effect of CYP3A4 on Liver Injury Induced by Triptolide.

Biomed Chromatogr 2020 Apr 24:e4864. Epub 2020 Apr 24.

State Key Laboratory of Phytochemistryand Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, China.

Triptolide (TP), one of the main bioactive diterpenes of the herbal medicine Tripterygium wilfordii Hook F, is used for treatment of autoimmune diseases in clinical companied by a severe hepatotoxicity. CYP3A4 has been reported to be responsible for TP metabolism, but the mechanism remains unclear. The present study applied an UPLC-QTOF-MS-based metabolomics analysis to characterize the effect of CYP3A4 on the TP-induced hepatotoxicity. Read More

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http://dx.doi.org/10.1002/bmc.4864DOI Listing

Phytochemical composition profile and space-time accumulation of secondary metabolites for Dracocephalum moldavica Linn. via UPLC-Q/TOF-MS and HPLC-DAD method.

Biomed Chromatogr 2020 Apr 24:e4865. Epub 2020 Apr 24.

Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, The MOE Key Laboratory for Standardization of Chinese Medicines, Shanghai R&D Centre for Standardization of Chinese Medicines, Shanghai, China.

The aerial parts of Dracocephalum moldavica L. are extensively used in traditional ethnic medicines in China as a remedy for cardiovascular and cerebrovascular damage. However, the chemical composition and the accumulation of main secondary metabolites of D. Read More

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http://dx.doi.org/10.1002/bmc.4865DOI Listing

High-throughput serum metabolomics analysis of gouty arthritis rat treated by total saponins of Rhizoma Dioscoreae Makino by UPLC-Q/TOF-MS.

Biomed Chromatogr 2020 Apr 24:e4867. Epub 2020 Apr 24.

Institute of Traditional Chinese Medicine, Heilongjiang University of Chinese Medicine, Harbin, China.

Rhizoma Dioscoreae Makino (RDM) is effective in treating gouty arthritis (GA) and hyperuricacidemia, especially in promoting uric acid excretion and reducing the inflammatory reaction. Bioactive constituents in RDM are mainly steroidal saponins such as dioscin, trillin, protodioscin and protogracillin. However, the mechanism of its anti-GA action is still unclear, owing to the complex pathological and physiological characteristics of GA, and integration of RDM with multiple components, multiple targets and multiple pathways. Read More

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http://dx.doi.org/10.1002/bmc.4867DOI Listing

A novel LC-MS/MS method for the determination of ziritaxestat in rat plasma and its pharmacokinetic study.

Biomed Chromatogr 2020 Apr 23:e4863. Epub 2020 Apr 23.

Department of Gastroenterology, The First People's Hospital of Lianyungang, Lianyungang, Jiangsu Province, China.

Ziritaxestat is a first-in-class autotoxin inhibitor. The purpose of this study was to develop a liquid chromatography/electrospray ionization tandem mass spectrometric (LC-MS/MS) method for the determination of ziritaxestat in rat plasma. The plasma sample was deproteinated using acetonitrile and then separated on an Acquity BEH C column with water containing 0. Read More

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http://dx.doi.org/10.1002/bmc.4863DOI Listing

In vivo metabolic investigation of cetilistat in normal versus pseudo-germ-free rats using UPLC-QTOFMS/MS and in silico toxicological evaluation of its metabolites.

Biomed Chromatogr 2020 Apr 20:e4860. Epub 2020 Apr 20.

Department of Pharmaceutical Analysis, National Institute of Pharmaceutical Education and Research (NIPER), Hyderabad, India.

Cetilistat (CET) is a pancreatic lipase inhibitor approved for management of obesity after the serious adverse effects exhibited by its analogue orlistat. Exhaustive literature review reveals lack of comprehensive reports on its biotransformation. With a view to study the same, the present study reports the identification and characterization of metabolites of CET in rats using UPLC-MS/MS. Read More

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http://dx.doi.org/10.1002/bmc.4860DOI Listing

The metabolites and biotransformation pathways in vivo after oral administrations of ocotillol, RT , and PF.

Biomed Chromatogr 2020 Apr 19:e4856. Epub 2020 Apr 19.

Department of Clinical Laboratory, The Second Affiliated Hospital of Dalian Medical University, Dalian, PR China.

Ocotillol, Pseudo-ginsenoside RT , and Pseudo-ginsenoside F are octilliol-type saponins, which have 0, 1, 2 of glucose at C-6 position with the same aglycone. In this paper, the metabolites of ocotilol, RT , and PF in rat plasma, stomach, intestine, urine, and feces after oral administrations were investigated with the method of ultra-performance liquid chromatography coupled with time of flight mass spectrometry (UPLC/QTOF-MS). The results showed that RT was easy to be transformed to metabolites in vivo. Read More

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http://dx.doi.org/10.1002/bmc.4856DOI Listing
April 2020
1.723 Impact Factor

An Improved LC-MS/MS Method for Determination of Docetaxel and Its Application to Population Pharmacokinetic Study in Chinese Cancer Patients.

Biomed Chromatogr 2020 Apr 19:e4857. Epub 2020 Apr 19.

Department of Pharmacy, the Third Affiliated Hospital (Gener Hospital), Chongqing Medical University, Chongqing, China.

Owing to its unpredictable side effects and efficacy, the anticancer drug docetaxel requires improved characterisation of its pharmacokinetic profiles through population pharmacokinetic (PPK) studies. A sensitive and rugged liquid chromatography-tandem mass spectrometry method for the detection of docetaxel in human plasma was developed and optimised using paclitaxel as an internal standard (IS). The plasma samples underwent rapid extraction using Hybrid solid-phase extraction-protein precipitation. Read More

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http://dx.doi.org/10.1002/bmc.4857DOI Listing
April 2020
1.723 Impact Factor

Residue analysis and kinetics modeling of thiophanate-methyl, carbendazim, tebuconazole and pyraclostrobin in apple tree bark using QuEChERS/HPLC-VWD.

Biomed Chromatogr 2020 Apr 19:e4851. Epub 2020 Apr 19.

College of Plant Health and Medicine, Qingdao Agricultural University, Qingdao, P.R. China.

Winter is the key period for the control of apple diseases, fungicides are needed to protect the trunk or main branches. Fungicide residue in apple tree bark is important basis for the action but there was still no report on their analytical methods and dissipation pattern. In this work, thiophanate-methyl, carbendazim, tebuconazole, and pyraclostrobin were selected as typical fungicides and a new QuEChERS-HPLC-VWD (QuEChERS extraction followed by high performance liquid chromatography detection with a variable wavelength detector) analytical method was developed to estimate their residue kinetics in apple tree bark during winter months. Read More

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http://dx.doi.org/10.1002/bmc.4851DOI Listing

LC-MS/MS assay for the quantification of foretinib in rat plasma and its application to preclinical pharmacokinetic study.

Biomed Chromatogr 2020 Apr 20:e4862. Epub 2020 Apr 20.

Department of Pharmacy, Jining Municipal Government Hospital, Jining, Shandong Province, China.

A simple and sensitive ultra-high-performance liquid chromatography tandem mass spectrometric method was developed and validated for the determination of foretinib in rat plasma. The analyte and internal standard were extracted from the bio-samples with acetonitrile and then separated on an Acquity UPLC BEH C column (50 × 2.1 mm, 1. Read More

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http://dx.doi.org/10.1002/bmc.4862DOI Listing

A rapid and sensitive bioanalytical LC-MS/MS method for the quantitation of a novel CDK5 inhibitor 20-223 (CP668863) in plasma: Application to in vitro metabolism and plasma protein-binding studies.

Biomed Chromatogr 2020 Apr 19:e4859. Epub 2020 Apr 19.

Clinical Pharmacology Laboratory, Department of Pharmacy Practice and Science, University of Nebraska Medical Center, Omaha, NE, USA.

A rapid, selective, and sensitive liquid chromatography coupled with tandem mass spectrometry (MS/MS) method was developed and validated for the quantitation of the novel CDK5 inhibitor '20-223' in mouse plasma. Separation of analytes was achieved by a reverse-phase ACE Excel C column (1.7 μm, 100 × 2. Read More

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http://dx.doi.org/10.1002/bmc.4859DOI Listing
April 2020
1.723 Impact Factor

Impurity profiling high-performance-thin-layer chromatography method involving the assay of essential human micronutrient niacin with eco-scale assessment.

Biomed Chromatogr 2020 Apr 19:e4858. Epub 2020 Apr 19.

Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Beni-Suef University, Beni-Suef, Egypt.

Currently, analytical scientists are paying special attention to reducing reliance on hazardous chemicals in various analytical methods. By embracing this concept, we developed an eco-friendly high-performancethin-layer chromatography (HPTLC) method as an alternative for the conventional HPLC method for the determination of an essential human micronutrient, niacin (NIA), which is used improve the lipid profile of patients. Furthermore, the proposed HPTLC method is capable of determining the structurally related impurities of NIA such as pyridine-2,5-dicarboxylic acid, isonicotinic acid, pyridine, and 5-ethyl-2-methylpyridine, which exhibit nephrotoxic and hepatotoxic effects. Read More

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http://dx.doi.org/10.1002/bmc.4858DOI Listing

Liquid chromatography-high resolution mass spectrometric method for the quantification of monomethyl auristatin E (MMAE) and its preclinical pharmacokinetics.

Biomed Chromatogr 2020 Apr 17:e4855. Epub 2020 Apr 17.

College of Pharmacy, Chungnam National University, Daejeon, South Korea.

MMAE is a potent antimitotic drug used as payload of an antibody-drug conjugate which shows potent activity in preclinical and clinical studies against a range of lymphomas, leukemia and solid tumors. Liquid chromatography-high resolution mass spectrometric method was developed for the quantification of MMAE and its preclinical pharmacokinetics. The method consisted of protein precipitation using acetonitrile (ACN) for sample preparation and liquid chromatography - quadrupole - time-of-flight - tandem mass spectrometry (LC-qTOF-MS/MS) analysis in the positive ion mode. Read More

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http://dx.doi.org/10.1002/bmc.4855DOI Listing

Novel HPTLC densitometric methods for determination of tamsulosin HCl and tadalafil in their newly formulated dosage form; Comparative study and green profile assessment.

Biomed Chromatogr 2020 Apr 17:e4850. Epub 2020 Apr 17.

Analytical Chemistry Department, Faculty of Pharmacy, Cairo University, Cairo, Egypt.

A promising combination of tamsulosin HCl and tadalafil has been recently introduced for treating two prevalent and associated urological disorders; benign prostate hyperplasia and erectile dysfunction. Novel HPTLC methods were designed and validated for assaying of the cited drugs in their challenging combined formulation. Separation was achieved using HPTLC silica gel 60 F plates as a stationary phase with a densitometric measurement at 280 nm. Read More

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http://dx.doi.org/10.1002/bmc.4850DOI Listing
April 2020
1.723 Impact Factor

Validation of a simplified procedure for convenient and rapid quantification of reduced and oxidized glutathione in human plasma by liquid chromatography tandem mass spectrometry analysis.

Biomed Chromatogr 2020 Apr 17:e4854. Epub 2020 Apr 17.

Research and Development, USANA Health Sciences, Inc., Salt Lake City, UT, United States.

Endogenous glutathione (GSH) and glutathione disulfide (GSSG) status are highly sensitive to oxidative conditions and have wide applications as surrogate indicators of redox status in vivo. Established methods for GSH and GSSG quantification in whole blood display limited utility in human plasma, where GSH and GSSG levels are ~3-4 orders of magnitude below that observed in whole blood. This study presents simplified sample processing and analytical LC-MS/MS approaches exhibiting the sensitivity and accuracy required to measure GSH and GSSG concentrations in human plasma samples, which after 5-fold dilution to suppress matrix interferences, range from 200-500 nM (GSH) and 5-30 nM (GSSG). Read More

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http://dx.doi.org/10.1002/bmc.4854DOI Listing

Identifying the chemical markers in raw and wine-processed Scutellaria baicalensis by ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry coupled with multiple statistical strategies.

Biomed Chromatogr 2020 Apr 17:e4849. Epub 2020 Apr 17.

Jiangxi University of Traditional Chinese Medicine, Nanchang, China.

Herb processing is a typical pharmaceutical preparation process for traditional Chinese medicine. After processing, its clinical applications and pharmacological effects vary greatly, which is most commonly attributed to the changing chemical properties between raw herb and processed products. In this work, a total of 53 chemical compounds were detected, among which 17 compounds were identified as discriminatory chemicals between raw and wine-processed Scutellaria baicalensis, and 10 components were identified as chemical markers with a cumulative content contribution of 88. Read More

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http://dx.doi.org/10.1002/bmc.4849DOI Listing

Development of a precise quantitative method for monitoring sirolimus in whole blood using LC/ESI-MS/MS.

Biomed Chromatogr 2020 Apr 17:e4853. Epub 2020 Apr 17.

Faculty of Pharmaceutical Sciences, Tohoku University, Sendai, Japan.

Sirolimus is used on patients after solid organ transplantation and on lymphangioleiomyomatosis (LAM) patients, and therapeutic drug monitoring is required in clinical practice. We have previously reported an accurate method for quantitative determination of sirolimus, but its sample preparation step was complicated. In this study, we developed a modified liquid chromatography/electrospray ionization tandem mass spectrometry (LC/ESI-MS/MS) method for sirolimus quantification. Read More

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http://dx.doi.org/10.1002/bmc.4853DOI Listing

Simultaneous measurement of epinastine and its metabolite, 9,13b-dehydroepinastine, in human plasma by a newly developed ultra-performance liquid chromatography-tandem mass spectrometry and its application to pharmacokinetic studies.

Biomed Chromatogr 2020 Apr 17:e4848. Epub 2020 Apr 17.

College of Pharmacy, Chonnam National University, Gwangju, Republic of Korea.

Epinastine is an antiallergic drug with high selectivity for histamine receptors. It has been reported that 9,13b-dehydroepinastine is present as a metabolite in vivo in humans, but there was little information about their pharmacokinetics (PKs) in humans. Although several analytical methods have been reported for epinastine analysis in different matrices, none are available for its metabolite. Read More

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http://dx.doi.org/10.1002/bmc.4848DOI Listing

Rapid screening and evaluation of XOD inhibitors and O scavenger from total flavonoids of Ginkgo biloba Leaves by LC-MS and multi-mode microplate reader.

Biomed Chromatogr 2020 Apr 17:e4852. Epub 2020 Apr 17.

National Center of Mass Spectrometry in Changchun & Jilin Province Key Laboratory of Chinese Medicine Chemistry and Mass Spectrometry & Chemical Biology Laboratory, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, China.

Superoxide anion radical scavenger and xanthine oxidase inhibitor plays an important function in the treatment of several relevant human diseases. In the present study, the ultrafiltration liquid chromatography-mass spectrometry coupled to microplate reader was applied to screen and identify superoxide anion radical scavengers and xanthine oxidase inhibitors from total flavonoids of G. biloba leaves. Read More

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http://dx.doi.org/10.1002/bmc.4852DOI Listing