624 results match your criteria Biomedical Research-tokyo[Journal]


Neutralizing antibodies for Helicobacter pylori urease inhibit bacterial colonization in the murine stomach in vivo.

Biomed Res 2019 ;40(2):87-95

Department of Microbiology and Immunology, Nippon Medical School.

Helicobacter pylori (H. pylori) urease is a key protein for persistent infection of the bacteria in the stomach. Although H. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.2220/biomedres.40.87DOI Listing
January 2019

Bush-like integrin filament networks associated with hyaloid vasculature in murine neonate eyes.

Biomed Res 2019 ;40(2):79-85

Laboratory of Histology and Cytology, Department of Anatomy, Hokkaido University Graduate School of Medicine.

The vitreous of perinatal mice temporarily develops a unique vascular system, called the vasa hyaloidea propria (VHP). Observations showed the vessels possessed an extracellular matrix including the basement membrane in their entire length. Immunostaining of whole mount preparations of VHP with integrin β antibody displayed a bush-like network consisting of long and straight fibers which were associated with the VHP but extended apart from the blood vessels. Read More

View Article

Download full-text PDF

Source
https://www.jstage.jst.go.jp/article/biomedres/40/2/40_79/_a
Publisher Site
http://dx.doi.org/10.2220/biomedres.40.79DOI Listing
January 2019
1 Read

Krüppel-like factor 5 (Klf5) regulates expression of mouse T1R1 amino acid receptor gene (Tas1r1) in C2C12 myoblast cells.

Biomed Res 2019 ;40(2):67-78

Division of Anatomy, Department of Health Promotion, Kyushu Dental University.

T1R1 and T1R3 are receptors expressed in taste buds that detect L-amino acids. These receptors are also expressed throughout diverse organ systems, such as the digestive system and muscle tissue, and are thought to function as amino acid sensors. The mechanism of transcriptional regulation of the mouse T1R1 gene (Tas1r1) has not been determined; therefore, in this study, we examined the function of Tas1r1 promoter in the mouse myoblast cell line, C2C12. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.2220/biomedres.40.67DOI Listing
January 2019

Dynamic changes in basal lamina fenestrations in rat intestinal villous epithelium under high-fat diet condition.

Biomed Res 2019 ;40(2):57-66

Division of Microscopic Anatomy, Niigata University Graduate School of Medical and Dental Sciences.

The basal lamina of the villous epithelium in the small intestine has numerous fenestrations, which are produced by leukocytes for their intraepithelial migration. We previously showed that these fenestrations change due to the dynamics of migrating leukocytes in response to dietary conditions and suggested the possibility that this change is related to the regulation of the absorption of large-sized nutrients such as chylomicrons. The present study was, thus, designed to investigate structural changes in basal lamina fenestrations in response to a high-fat diet. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.2220/biomedres.40.57DOI Listing
January 2019

Comparison of immune response in mice sensitized to an animal allergen, Can f 1, and to a food allergen, ovalbumin.

Biomed Res 2019 ;40(1):9-15

Institute for Animal Experimentation, Hokkaido University.

Can f 1 belongs to the lipocalin superfamily and is considered to be an animal allergen. The immune response induced by Can f 1 in mice was compared with that induced by ovalbumin (OVA), a typical food allergen. Female BALB/c and C57BL/6 mice (6 weeks of age) were subcutaneously injected with Can f 1 or OVA with or without aluminum hydroxide (Alum) three times with intervals of two weeks. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.2220/biomedres.40.9DOI Listing
January 2019
3 Reads

Histone H1 quantity determines the efficiencies of apoptotic DNA fragmentation and chromatin condensation.

Biomed Res 2019 ;40(1):51-56

Research Institute for Biomedical Sciences, Tokyo University of Science.

Oligonucleosomal DNA fragmentation and chromatin condensation are two hallmarks of apoptosis. However, their generation mechanisms are not entirely understood. Histone H1, a positively charged nuclear protein located in the linker region of chromatin, is involved in higher-order chromatin structures and tight chromatin packing. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.2220/biomedres.40.51DOI Listing
January 2019

Dominant-negative p53 mutant R248Q increases the motile and invasive activities of oral squamous cell carcinoma cells.

Biomed Res 2019 ;40(1):37-49

Department of Gerodontology, Division of Oral Health Science, Graduate School of Dental Medicine.

The tumor suppressor gene TP53 (gene) codes for a transcription factor which transactivates its target genes responsible for cell cycle arrest, DNA repair, apoptosis, and senescence. TP53 is well known to be the most frequent target of genetic mutations in nearly half of human cancers including oral squamous cell carcinoma (OSCC). Many p53 mutants including R248Q and R248W not only lose its tumor-suppressor activities, but also interfere with the functions of wild-type p53; this is so-called dominant-negative (DN) mutation. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.2220/biomedres.40.37DOI Listing
January 2019

Exhaled acetone and isoprene in perioperative lung cancer patients under intensive oral care: possible indicators of inflammatory responses and metabolic changes.

Biomed Res 2019 ;40(1):29-36

Division of Preventive Dentistry, Department of Oral Health and Development Sciences, Tohoku University Graduate School of Dentistry.

One of the most severe complications of lung resection is postoperative pneumonia, and its prevention and prediction are critical. Exhaled acetone and isoprene are thought to be related to metabolism; however, little is known on their relationship with bacteria living in the oral cavity or their meaning in the acute phase in perioperative lung cancer patients. We measured acetone and isoprene in exhaled breath of 13 Japanese patients with lung cancer (3 women and 10 men, age range 62-82 years, mean 72. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.2220/biomedres.40.29DOI Listing
January 2019
1 Read

Clinicopathological and prognostic significance of nuclear UGDH localization in lung adenocarcinoma.

Biomed Res 2019 ;40(1):17-27

Department of Applied Tumor Pathology, Graduate School of Medical Sciences, Kitasato University.

This study aimed to clarify relationships among UDP-glucose-6 dehydrogenase (UGDH) expression, clinicopathological factors, and the prognosis of patients, and to determine the role of UGDH in lung adenocarcinoma (AC). Firstly, UGDH expression and localization in 126 lung AC tissues were immunohistochemically studied, and associations with clinicopathological parameters and patients' prognosis were evaluated. Secondly, serum UGDH levels were measured in 267 lung cancer patients and 100 healthy controls. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.2220/biomedres.40.17DOI Listing
January 2019

Citrulline as a novel adjuvant candidate for vaccines.

Biomed Res 2019 ;40(1):1-7

Department of Medical Technology, Kumamoto Health Science University.

For a long time, many types of vaccines have been useful for the prophylaxis of many infectious diseases. Thus far, many adjuvants that enhance the effects of vaccines have been explored. However, very few adjuvants are being used for humans worldwide. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.2220/biomedres.40.1DOI Listing
January 2019

A role for scavenger receptor B1 as a captor of specific fatty acids in taste buds of circumvallate papillae.

Biomed Res 2018 ;39(6):295-300

Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University.

Class B scavenger receptor family members, scavenger receptor B1 (SR-B1) and cluster of differentiation 36 (CD36), are broadly expressed cell-surface proteins, both of which are believed to serve as multifaceted players in lipid and lipoprotein metabolism in mammals. Because of its presence in the apical part of taste receptor cells within circumvallate taste buds and its ability to recognise long-chain fatty acids, CD36 has been believed to participate in the sensing of the lipid species within the oral cavity. However, there have been no attempts to address whether SR-B1 has such a role to date. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.2220/biomedres.39.295DOI Listing

Vascularization via activation of VEGF-VEGFR signaling is essential for peripheral nerve regeneration.

Biomed Res 2018 ;39(6):287-294

Division of Dental Anesthesiology, Niigata University Graduate School of Medical and Dental Sciences.

Peripheral nerve injury has been suggested to up-regulate mRNA for the vascular endothelial growth factor (VEGF) which enhances nerve regeneration. VEGF is known to regulate angiogenesis by binding with a specific receptor, the vascular endothelial growth factor receptor (VEGFR). However, little is known about the involvement of VEGF-VEGFR signaling in the nerve regeneration at early stages though previous studies contained a lengthy observation. Read More

View Article

Download full-text PDF

Source
https://www.jstage.jst.go.jp/article/biomedres/39/6/39_287/_
Publisher Site
http://dx.doi.org/10.2220/biomedres.39.287DOI Listing
April 2019
13 Reads

Differences in the effects of TRPV1 antagonists on energy metabolism in mice.

Biomed Res 2018 ;39(6):279-286

Laboratory of Functional Anatomy, Faculty of Agriculture, Kyushu University.

Transient receptor potential vanilloid 1 (TRPV1) is a nociceptive cation channel that is activated by heat, protons and chemical ligands such as capsaicin. We investigated the roles of the capsaicin receptor, TRPV1, in controlling the energy metabolism of the whole body. It has been reported that the activation of TRPV1 by its agonists enhances energy metabolism. Read More

View Article

Download full-text PDF

Source
https://www.jstage.jst.go.jp/article/biomedres/39/6/39_279/_
Publisher Site
http://dx.doi.org/10.2220/biomedres.39.279DOI Listing
April 2019
10 Reads

Pelvic venous congestion induces lower urinary tract dysfunction in rats.

Biomed Res 2018 ;39(6):269-277

Department of Biochemistry, Graduate School of Medicine, University of the Ryukyus.

Pelvic venous congestion (PC) is thought to be related to several diseases of the lower urinary tract (LUT). We examined the characteristics of the LUT in rats with PC. To create PC, female rats were anesthetized with isoflurane, and the bilateral common iliac veins and bilateral uterine veins were ligated. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.2220/biomedres.39.269DOI Listing

Analgesic effects of minodronate in a rat chronic pain model.

Biomed Res 2018 ;39(5):261-268

Department of Orthopedic Surgery, Akita University Graduate School of Medicine.

We evaluated the analgesic effects of minodronate, alendronate and pregabalin on mechanical and thermal allodynia, as well as changes in bone mineral density and skeletal muscle volume caused by chronic constriction injury (CCI) in an ovariectomized rat. Ovariectomy was performed on four-week-old female Wistar rats. Thereafter, at 8-weeks of age, the left sciatic nerve was ligated to create the chronic pain model (CCI limb), and sham surgery was performed on the right hindlimb. Read More

View Article

Download full-text PDF

Source
https://www.jstage.jst.go.jp/article/biomedres/39/5/39_261/_
Publisher Site
http://dx.doi.org/10.2220/biomedres.39.261DOI Listing
December 2018
11 Reads

1O, 20O-diacetyl kamebakaurin protects against acetaminophen-induced hepatotoxicity in mice.

Biomed Res 2018 ;39(5):251-260

College of Pharmacy, Kinjo Gakuin University.

The present study aimed to investigate the protective effects of kamebakaurin (KA) and 1O, 20O-diacetyl kamebakaurin (AcKA) on acetaminophen (APAP)-induced hepatotoxicity and compare the hepatoprotective mechanisms of the two chemicals. Seven-week-old male C57BL/6J mice were orally administered KA, AcKA, or an ethanol/olive oil emulsion once per day for 7-days. Twenty-four hours after the final administration, the mice were fasted and then intraperitoneally injected with 450 mg/kg APAP or saline. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.2220/biomedres.39.251DOI Listing
December 2018
1 Read

Inhalation of Japanese cedar (Cryptomeria japonica) wood odor causes psychological relaxation after monotonous work among female participants.

Biomed Res 2018 ;39(5):241-249

Kansai Research Center, Forestry and Forest Products Research Institute.

Essential oils have potential to mitigate stress symptoms and treat symptoms related to mental health. Few studies have investigated the effects of wood-derived aromatics on endocrinological and psychological responses in an actual space. In this study, we evaluated the effects of essential oil derived from Japanese cedar (Cryptomeria japonica) wood on the recovery state of female participants after they performed monotonous work. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.2220/biomedres.39.241DOI Listing
December 2018

Gnetin C suppresses double-stranded RNA-induced C-C motif chemokine ligand 2 (CCL2) and CCL5 production by inhibiting Toll-like receptor 3 signaling pathway.

Biomed Res 2018 ;39(5):231-240

Department of Pediatrics, Hirosaki University Graduate School of Medicine.

The innate immune system is a prerequisite for biophylactic ability, but its dysregulation can cause inflammatory and autoimmune diseases. To determine a safe method of controlling inflammatory reactions in the brain, we examined the effects of gnetin C, a natural resveratrol dimer, on C-C motif chemokine ligand 2 (CCL2) and CCL5 (pro-inflammatory chemokines) production observed after treatment with polyinosinic-polycytidylic acid [poly IC; a synthetic analog of dsRNA as a Toll-like receptor 3 (TRL3) ligand, 30 μg/mL] in cultured human astrocytoma U373MG and neuroblastoma SH-SY5Y cells. The addition of gnetin C (10 μM) to the media moderately reduced the CCL2 production and markedly suppressed CCL5 production in both cells. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.2220/biomedres.39.231DOI Listing
December 2018
4 Reads

Sevoflurane inhibits presynaptic calcium influx without affecting presynaptic action potentials in hippocampal CA1 region.

Biomed Res 2018 ;39(5):223-230

Deptartment of Anesthesiology and Critical Care Medicine, Faculty of Medicine and Graduate School of Medicine Hokkaido University.

Although diverse effects of volatile anesthetics have been investigated in various studies, the mechanisms of action of such anesthetics, especially sevoflurane, remain elusive. In contrast to their potent modulation of inhibitory synaptic transmission there is little information about their effects on excitatory transmission in the brain. In this study, we examined the effect of sevoflurane on the excitatory synaptic transmission at CA1 synapses in hippocampal slices of mice. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.2220/biomedres.39.223DOI Listing
December 2018

Analgesic effect of mineral cream containing natural spa minerals for use on the skin.

Biomed Res 2018 ;39(4):215-222

Global Research Center for Innovative Life Science, Hoshi University.

Previous studies have shown that dissolved substances in some natural hot springs have analgesic/anti-nociceptive and anti-inflammatory actions. However, the mechanisms underlying how such dissolved substances exert these actions are not fully understood. In the present study on mice, we examined the analgesic/anti-nociceptive and anti-inflammatory properties of a mineral cream containing natural hot spring ingredients. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.2220/biomedres.39.215DOI Listing
November 2018
9 Reads

Differential effects of depot formulations of GnRH agonist leuprorelin and antagonist degarelix on the seminiferous epithelium of the rat testis.

Biomed Res 2018 ;39(4):197-214

Department of Microscopic Anatomy and Cell Biology, Asahikawa Medical University.

Despite their pharmacologically opposite actions, long-acting depot formulations of both GnRH agonists and antagonists have been clinically applied for treatment of androgen-sensitive prostate cancer. Sustained treatment with GnRH analogues commonly suppresses both the synthesis and release of gonadotropins, leading to depletion of testicular testosterone. To clarify the underlying differences in the effects of GnRH agonists and antagonists on spermatogenesis, we compared histological changes in the seminiferous epithelium after administration of depot formulations of GnRH agonist leuprorelin and antagonist degarelix to male rats. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.2220/biomedres.39.197DOI Listing
November 2018
1 Read

Neuron-like cells in the chick spinal accessory lobe express neuronal-type voltage-gated sodium channels.

Biomed Res 2018 ;39(4):189-196

Laboratory of Veterinary Physiology, Faculty of Agriculture, Tottori University.

Ten pairs of protrusions, called accessory lobes (ALs), exist at the lateral sides of the avian lumbosacral spinal cord. Histological evidence indicates that neuron-like cells gather in the ALs, and behavioral evidence suggests that the ALs act as a sensory organ of equilibrium during bipedal walking. Recently, using an electrophysiological method, we reported that cells showing Na currents and action potentials exist among cells that were dissociated from the ALs. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.2220/biomedres.39.189DOI Listing
November 2018
1 Read

Intracellular localization of GGA accessory protein p56 in cell lines and central nervous system neurons.

Biomed Res 2018 ;39(4):179-187

Department of Anatomy and Histology, Fukushima Medical University School of Medicine.

Adaptor protein complex-1 (AP-1) and Golgi associated, γ-adaptin ear containing, Arf binding proteins (GGAs) are clathrin adaptors that regulate membrane trafficking between the trans-Golgi network (TGN) and endosomes. p56 is a clathrin adaptor accessory protein that may modulate the function of GGAs in mammalian cell lines. However, the precise relationship between p56 and the three GGAs (GGA1-3), as well as the physiological role of p56 in tissue cells, remain unknown. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.2220/biomedres.39.179DOI Listing
November 2018

Dihydroartemisinin represses osteoclastogenesis of bone marrow macrophages through reduced NFATc1 expression and impaired phosphorylation of IκBα.

Biomed Res 2018 ;39(4):169-177

Department of Dental Pharmacology, Nagasaki University Graduate School of Biomedical Sciences.

Osteoclasts are multinucleated bone resorbing cells whose differentiation is regulated by several important signaling pathways. Several lines of evidence indicate that dihydroartemisinin (DHA), an anti-malarial drug, inhibits osteoclast differentiation with little cytotoxicity. However, the detailed inhibitory mechanisms of DHA on osteoclastogenesis from native cells remain to be elucidated. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.2220/biomedres.39.169DOI Listing
November 2018
1 Read

Tumor mutational burden analysis of 2,000 Japanese cancer genomes using whole exome and targeted gene panel sequencing.

Biomed Res 2018 ;39(3):159-167

Shizuoka Cancer Center.

Tumor mutational burden (TMB) is an emerging characteristic in cancer and has been associated with microsatellite instability, defective DNA replication/repair, and response to PD-1 and PD-L1 blockade immunotherapy. When estimating TMB, targeted panel sequencing is performed using a few hundred genes; however, a comparison of TMB results obtained with this platform and with whole exome sequencing (WES) has not been performed for various cancer types. In the present study, we compared TMB results using the above two platforms in 2,908 solid tumors that were obtained from Japanese patients. Read More

View Article

Download full-text PDF

Source
https://www.jstage.jst.go.jp/article/biomedres/39/3/39_159/_
Publisher Site
http://dx.doi.org/10.2220/biomedres.39.159DOI Listing
October 2018
19 Reads

Up-regulation of DUSP5 and DUSP6 by gonadotropin-releasing hormone in cultured hypothalamic neurons, GT1-7 cells.

Biomed Res 2018 ;39(3):149-158

Department of Biochemistry, Graduate School of Medicine, University of the Ryukyus.

Gonadotropin-releasing hormone (GnRH) is secreted from hypothalamic neurons (GnRH neurons) and stimulates anterior pituitary gonadotrophs to synthesize and secrete gonadotropins. In addition to gonadotrophs, GnRH neurons also express GnRH receptors, and the autocrine action of GnRH is reportedly involved in the regulation of functions of GnRH neurons. There is accumulating evidence that extracellular signal-regulated kinase (ERK), one of mitogen-activated protein kinases (MAPKs), is activated by GnRH and involved in various effects of GnRH in GnRH neurons. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.2220/biomedres.39.149DOI Listing
October 2018

Docosahexaenoic acid suppresses angiotensin II-induced A7r5 vascular smooth muscle cell proliferation and migration under pulsatile pressure stress.

Biomed Res 2018 ;39(3):141-148

Elevated mechanical stress applied to vascular walls is well known to modulate vascular remodeling and plays a part in the pathogenesis of atherosclerosis. On the other hand, docosahexaenoic acid (DHA), an n-3 polyunsaturated fatty acid, has been shown to protect against several types of cardiovascular diseases including atherosclerosis and hypertension. The aim of this study was to clarify the effect of pulsatile pressure stress and DHA on angiotensin II-induced proliferation and migration in A7r5 vascular smooth muscle cells (VSMCs). Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.2220/biomedres.39.141DOI Listing
October 2018
1 Read

Effects of the calcineurin inhibitors cyclosporine and tacrolimus on bone metabolism in rats.

Biomed Res 2018 ;39(3):131-139

Department of Clinical Pharmacotherapy, Faculty of Pharmaceutical Sciences, Niigata University of Pharmacy and Applied Life Sciences.

Immunosuppressive therapy is considered as one of the factors inducing to the onset of osteoporosis after organ transplantation. Chronic immunosuppressive therapy after transplantation is required for organ transplant patients, and it is important to prevent the occurrence of osteoporotic fractures to maintain the quality of life in patients. In this study, we examined the effects of cyclosporine and tacrolimus on bone metabolism in rats. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.2220/biomedres.39.131DOI Listing
October 2018
1 Read

A novel role for scavenger receptor B1 as a contributor to the capture of specific volatile odorants in the nasal cavity.

Biomed Res 2018 ;39(3):117-129

Laboratory of Nutrition Chemistry, Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University.

Class B scavenger receptors, scavenger receptor B1 (SR-B1) and cluster of differentiation 36 (CD36), are broadly expressed cell-surface proteins and are believed to serve as multifaceted players in lipid and lipoprotein metabolism in mammals. Because of its ability to recognise distinct odour-active volatile compounds and its presence in murine olfactory epithelium, CD36 has recently emerged as a participant in the detection of odorants within the nasal cavity. However, there have been no attempts to assess whether SR-B1 has such a role. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.2220/biomedres.39.117DOI Listing
October 2018
4 Reads

Gnetin C, a resveratrol dimer, reduces amyloid-β 1-42 (Aβ42) production and ameliorates Aβ42-lowered cell viability in cultured SH-SY5Y human neuroblastoma cells.

Biomed Res 2018 ;39(3):105-115

Department of Vascular Biology, Institute of Brain Science, Hirosaki University Graduate School of Medicine.

Accumulation and oligomerization of amyloid-beta (Aβ) peptides have been known to be a potent cause of neurodegenerative diseases such as Alzheimer's disease (AD). To expand the possibilities of preventing AD, we investigated the effects of resveratrol dimers, gnetin C and ε-viniferin, on Aβ 1-42 (Aβ42) production and the reduced cell viability observed after Aβ42 treatment (monomers, 10 μM) in cultured SH-SY5Y human neuroblastoma cells. Among them, addition of gnetin C (20 μM) into the media reduced Aβ42 production most efficiently. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.2220/biomedres.39.105DOI Listing
October 2018
7 Reads

Establishment of protocol for preparation of gene-edited bovine ear-derived fibroblasts for somatic cell nuclear transplantation.

Biomed Res 2018 ;39(2):95-104

Department of Veterinary Medicine, School of Veterinary Medicine, Rakuno Gakuen University.

Recently, gene-editing using the clustered regularly interspaced short palindromic repeats (CRISPR)/ CRISPR-associated protein 9 (Cas9) technique has attempted to utilize fibroblasts of livestock animals for somatic cell nuclear transfer. In this study, we establish the procedure for preparing skin fibroblast clones whose genes were edited by the CRISPR/Cas9 technique. After isolating fibroblasts from earlobes of Japanese Black cattle, subsequent collagenase-digestion and extensive wash procedures enabled us to avoid contamination of fungi. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.2220/biomedres.39.95DOI Listing
September 2018

3-methyadenine attenuates chloroform-induced hepatotoxicity via autophagy activation.

Biomed Res 2018 ;39(2):87-94

Teaching and Research Centre, Faculty of Medicine, Xinyang Vocational and Technical College.

Chloroform is a common contaminant in the drinking water. Exposure of human to chloroform leads to severe hepatotoxicity. In the present study, chloroform-induced acute liver injury was investigated in mice using 3-methyadenine (3-MA), a common autophagy inhibitor. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.2220/biomedres.39.87DOI Listing
September 2018

Quantitative analyses of the metaphase-to-anaphase transition reveal differential kinetic regulation for securin and cyclin B1.

Biomed Res 2018 ;39(2):75-85

Division of Experimental Pathology, Cancer Institute of the Japanese Foundation for Cancer Research (JFCR).

Separation of sister chromatids is a drastic and irreversible step in the cell cycle. The key biochemistry behind this event is the proteolysis mediated by the ubiquitin ligase called the anaphase promoting complex, or APC/C. Securin and cyclin B1 are the two established substrates for APC/C whose degradation releases separase and inactivates cyclin B1-dependent kinase 1 (cdk1), respectively, at the metaphase-to-anaphase transition. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.2220/biomedres.39.75DOI Listing
September 2018
7 Reads

Dynamics of basal lamina fenestrations in the rat intestinal villous epithelium in response to dietary conditions.

Biomed Res 2018 ;39(2):65-74

Division of Microscopic Anatomy, Niigata University Graduate School of Medical and Dental Sciences.

The epithelial basal lamina of the small intestine forms a felt-like sheet at the base of the epithelium. Previous studies have shown that the basal lamina has numerous fenestrations, which are produced by leukocytes penetrating through the basal lamina. In this study, we aimed to directly visualize fenestrations of the rat basal lamina in intestinal villi by scanning electron microscopy (SEM) after removal of the villous epithelium by osmium maceration and ultrasonic treatment. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.2220/biomedres.39.65DOI Listing
September 2018
1 Read

Circadian rhythms of micturition during jet lag.

Biomed Res 2018 ;39(2):57-63

Department of Systems Biology, Graduate School of Pharmaceutical Sciences, Kyoto University.

Micturition behavior follows regular day/night fluctuations, and unwanted increase in micturition could occur during night in jet lag condition. To clarify the effect of jet lag on micturition behavior, we simultaneously detected circadian micturition patterns and locomotor activity rhythms of mice under experimental jet lag conditions, by applying the improved automated Voided Stain on Paper (aVSOP) method. When wild-type (WT) mice were phase-advanced for 8 hours, day-night variation of micturition was disrupted suddenly, and this irregular daily micturition continued until 8 days, although their activity rhythms entrained gradually day by day until 8 days. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.2220/biomedres.39.57DOI Listing
September 2018
2 Reads

Short and long photoperiods differentially exacerbate corticosterone-induced physical and psychological symptoms in mice.

Biomed Res 2018 ;39(1):47-55

Faculty of Pharmaceutical Sciences, Josai International University.

Circadian disruption affects the pathogenesis and development of various diseases. Depression is one of the most common diseases that relate to circadian rhythm. In this study, we analyzed the effects of daily light/dark (LD) conditions on depression and other symptoms, and also analyzed the mixed effects of LD conditions and corticosterone treatment. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.2220/biomedres.39.47DOI Listing
June 2018
2 Reads

Influence of nitrous oxide on granule cell migration in the dentate gyrus of the neonatal rat.

Biomed Res 2018 ;39(1):39-45

For several decades, the neurotoxicities of anesthetics to the developing brain have been reported by many researchers focusing on various phenomena such as apoptosis, neurodegeneration, electrophysiological aberrations, and behavioral abnormalities. According to these reports, signals via N-methyl-D-aspartate receptors (NMDA-r) and/or γ-aminobutyric acid type A receptors (GABA-r) are implicated in the anesthetic neurotoxicity. On the other hand, during brain development, NMDA-r and GABA-r are also recognized to play primary roles in neural cell migration. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.2220/biomedres.39.39DOI Listing

Targeting of severe fever with thrombocytopenia syndrome virus structural proteins to the ERGIC (endoplasmic reticulum Golgi intermediate compartment) and Golgi complex.

Biomed Res 2018 ;39(1):27-38

Laboratory of Public Health, Department of Preventive Veterinary Science, Division of Veterinary Medicine, Faculty of Veterinary Medicine, Hokkaido University.

Severe fever with thrombocytopenia syndrome phlebovirus (SFTSV) is a newly emerged phlebovirus identified in China, Japan, and South Korea. Phlebovirus glycoproteins (GP) play a key role in targeting viral structural components to the budding compartments in the ER-Golgi intermediate compartment (ERGIC) and Golgi complex. However, the role of SFTSV GP in targeting structural proteins to the ERGIC and Golgi complex remains unresolved. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.2220/biomedres.39.27DOI Listing
June 2018
4 Reads

Combination of a cryosectioning method and section scanning electron microscopy for immuno-scanning electron microscopy.

Biomed Res 2018 ;39(1):21-25

Division of Morphological Sciences, Kagoshima University Graduate School of Medicine and Dental Sciences.

We describe a novel immuno-scanning electron microscopy (SEM) technique that combines both Tokuyasu's cryosectioning and section SEM methods. In this technique, semithin cryosections, cut according to the Tokuyasu method, were adhered to glass microscope slides, immunostained for bio-molecules of interest and observed by confocal laser scanning microscopy. The same sections were subsequently embedded in epoxy resin and ultrathin sections were cut on an ultramicrotome. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.2220/biomedres.39.21DOI Listing
June 2018
1 Read

A cell-based interferon-tau assay with an interferon-stimulated gene 15 promoter.

Biomed Res 2018 ;39(1):13-20

Cooperative Department of Veterinary Medicine, Faculty of Agriculture, Iwate University.

Interferon-tau (IFNT) is known as an early pregnancy recognition signal in ruminants. An accurate and convenient IFNT detection system is desirable for the diagnosis of endometrial and trophoblastic functions, including gestation status, in cows. The aim of this study was to develop a new cell-based assay, which involved the stable introduction of an interferon-stimulated gene promoter to a luciferase reporter system. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.2220/biomedres.39.13DOI Listing
June 2018
1 Read

Absorption and metabolism of orally administered collagen hydrolysates evaluated by the vascularly perfused rat intestine and liver in situ.

Biomed Res 2018 ;39(1):1-11

Faculty of Agriculture, Utsunomiya University.

A number of studies have shown that oral administration of collagen hydrolysate (CH) results in the absorption of di- and tri-peptides. In order to understand the dynamics of CH absorption and metabolism, molecular profiles of hydroxyproline (Hyp) and Hyp-containing peptides (HCPs) were analyzed by in situ perfusion of rat intestine and liver. The total amount of absorbed HCPs during 1 h of perfusion was 16. Read More

View Article

Download full-text PDF

Source
https://www.jstage.jst.go.jp/article/biomedres/39/1/39_1/_ar
Publisher Site
http://dx.doi.org/10.2220/biomedres.39.1DOI Listing
June 2018
10 Reads

Cognitive impairment with interferon treatment in patients with chronic hepatitis C.

Biomed Res 2017 ;38(6):371-374

Interferon (IFN) has various side effects, including psychiatric symptoms. Event-related potentials are used as an electrophysiologic index of cognitive disorders. Auditory event-related potentials (P300) are often used in conditions in which cognitive ability is affected. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.2220/biomedres.38.371DOI Listing
July 2018
2 Reads

Noradrenergic effects in rat sacral autonomic nucleus using in vitro slice patch-clamp recordings.

Biomed Res 2017 ;38(6):359-369

Department of Neurophysiology, Akita University, School of Medicine.

Noradrenergic modulation has been frequently discussed in the context of neural activities that are related to pelvic organs. The sacral preganglionic nucleus (SPN) is a spinal nucleus containing parasympathetic preganglionic neurons that send fibers to pelvic nerves. In spite of the abundant presence of noradrenergic fibers around the SPN, the effects of noradrenaline (NA) remain obscure. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.2220/biomedres.38.359DOI Listing
July 2018
1 Read

Fish protein hydrolysate exhibits anti-obesity activity and reduces hypothalamic neuropeptide Y and agouti-related protein mRNA expressions in rats.

Biomed Res 2017 ;38(6):351-357

Department of Applied Biological Chemistry, Faculty of Agriculture, Utsunomiya University.

Fish protein is a source of animal protein that is consumed worldwide. Although it has been reported that the intake of Alaska pollack protein (APP) reduces body fat accumulation and increases muscle weight in rats, the mechanisms underlying these effects are poorly understood. As a possibility, peptides released from APP in the gastrointestinal tract are important to the functions of APP. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.2220/biomedres.38.351DOI Listing

Iron deficiency induces autophagy and activates Nrf2 signal through modulating p62/SQSTM.

Biomed Res 2017 ;38(6):343-350

Department of Nutritional Science and Food Safety, Tokyo University of Agriculture.

Iron is an essential trace metal in almost all organisms and plays an important role in the redox system. We previously reported that iron deficiency activated autophagy and nuclear factor erythroid 2-related factor 2 (Nrf2) signaling for oxidative stress. However, regulatory mechanisms underlying the association between autophagy and Nrf2 signaling are unclear. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.2220/biomedres.38.343DOI Listing
July 2018
17 Reads

Changes in FOXO and proinflammatory cytokines in the late stage of immobilized fast and slow muscle atrophy.

Biomed Res 2017 ;38(6):331-342

Graduate School of Health and Sports Science, Juntendo University.

The present study aimed to examine the changes in the expression of Forkhead box protein O (FOXO) and proinflammatory cytokines in both slow-twitch soleus and fast-twitch plantaris muscles following cast immobilization. Male C57BL/6 mice were subjected to cast immobilization for 7 and 21 days. Cast immobilization increased FOXO3a mRNA and total protein expression in both the soleus and plantaris muscles. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.2220/biomedres.38.331DOI Listing
July 2018
6 Reads

Inhibitory role of Munc13-1 in antigen-induced mast cell degranulation.

Biomed Res 2017 ;38(6):321-329

Department of Anatomy (Cell Biology), Iwate Medical University.

Secretory granules (SGs) of mast cells are lysosome-related organelles that contain various inflammatory molecules such as histamine, which are stored in the cytoplasm. Mast cell degranulation is the regulated exocytosis of SGs in response to external stimuli, such as the antigen-mediated cross-linking of the high-affinity IgE receptor, FcεRI. Upon stimulation, SGs undergo priming to become fusion-competent prior to fusing with the plasma membrane, which is mediated by Munc13-4, one of the five members of the vesicle-priming Munc13 protein family. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.2220/biomedres.38.321DOI Listing
July 2018
5 Reads

Cordycepin induces apoptotic cell death and inhibits cell migration in renal cell carcinoma via regulation of microRNA-21 and PTEN phosphatase.

Biomed Res 2017 ;38(5):313-320

Department of Urology, The First Affiliated Hospital of Zhengzhou University.

Cordycepin is an active component extracted from Traditional Chinese medical herb Cordyceps militaris. Many reports demonstrated that cordycepin harbors antitumor activity in a broad spectrum of cancer types. In this study the actions and the underneath molecular mechanisms of cordycepin were investigated in renal cell carcinoma Caki-1 cell line. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.2220/biomedres.38.313DOI Listing
June 2018
10 Reads

No change in the prevalence of latent prostate cancer over the last 10 years: a forensic autopsy study in Japan.

Biomed Res 2017 ;38(5):307-312

Department of Pathology, Shiga University of Medical Science.

Although the morbidity rate of prostate cancer has increased with 2.3 times in these 10 years in Japan, little is known about the changes in the prevalence of latent prostate cancer. To understand changes in the prevalence of latent prostate cancer, a retrospective analysis was performed. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.2220/biomedres.38.307DOI Listing
June 2018
6 Reads

Effects of the antiepileptic drugs topiramate and lamotrigine on bone metabolism in rats.

Biomed Res 2017 ;38(5):297-305

Department of Clinical Pharmacotherapy, Faculty of Pharmaceutical Sciences, Niigata University of Pharmacy and Applied Life Sciences.

Long-term treatment with antiepileptic drugs (AED) is associated with an elevated risk of bone fracture due to decreased bone mineral density (BMD). Phenytoin has been shown to affect bone metabolism adversely, whereas newly developed AEDs have not been studied. This study evaluated the effects of topiramate and lamotrigine on bone metabolism in rats. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.2220/biomedres.38.297DOI Listing
June 2018
22 Reads