1,577 results match your criteria Biomedical Microdevices [Journal]


An immunoassay cassette with a handheld reader for HIV urine testing in point-of-care diagnostics.

Biomed Microdevices 2020 May 21;22(2):39. Epub 2020 May 21.

Institute of Microfluidic Chip Development in Biomedical Engineering, College of Information Science and Technology, Beijing University of Chemical Technology, Beijing, 100029, China.

Currently, most HIV tests are performed with blood samples, or alternatively saliva samples are used for HIV testing. Simple HIV tests need to be performed in hospitals or other medical agencies instead of more invasive HIV blood tests. To enable point-of-care (POC) HIV diagnostics, based on a recently developed lateral flow strip for HIV urine testing, a microfluidic immunoassay cassette with a handheld optical reader is developed. Read More

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http://dx.doi.org/10.1007/s10544-020-00494-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7239691PMC

On-chip estimation of hematocrit level for diagnosing anemic conditions by Impedimetric techniques.

Biomed Microdevices 2020 May 20;22(2):38. Epub 2020 May 20.

Department of Electronic Science, University of Calcutta, 92 A.P.C. Road, Kolkata, 700009, India.

An on-chip device has been fabricated on Si platform for precise estimation of the hematocrit (Hct) level of human blood with rapid turn out. Impedance/capacitance spectroscopy and current-voltage (I-V) measurements have been employed to observe the variation of electrical parameters of erythrocyte suspensions with varying Hct level. Experimentally obtained values of capacitance, impedance and conductance with Hct level suggests a linear variation. Read More

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http://dx.doi.org/10.1007/s10544-020-00493-5DOI Listing

Development and characterization of a PDMS-based masking method for microfabricated Oral drug delivery devices.

Biomed Microdevices 2020 May 18;22(2):35. Epub 2020 May 18.

The Danish National Research Foundation and Villum Foundation's Center for Intelligent Drug Delivery and Sensing Using Microcontainers and Nanomechanics, Department of Health Technology, Technical University of Denmark, 2800, Kgs. Lyngby, Denmark.

With the growing popularity and application of microfabricated devices in oral drug delivery (ODD), masking technologies for drug loading and surface modification become highly relevant. Considering the speed of design and fabrication processes and the necessity for continuous alterations of e.g. Read More

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http://dx.doi.org/10.1007/s10544-020-00490-8DOI Listing

Simultaneous electrical detection of IL-6 and PCT using a microfluidic biochip platform.

Biomed Microdevices 2020 May 18;22(2):36. Epub 2020 May 18.

Department of Bioengineering, University of Illinois at Urbana-Champaign, 1102 Everitt Lab, MC 278, 1406 W. Green St, Urbana, IL, 61801, USA.

Sepsis, a life-threatening organ dysfunction caused by a dysregulated host response, leads the U.S in both mortality rate and cost of treatment. Sepsis treatment protocols currently rely on broad and non-specific parameters like heart and respiration rate, and temperature; however, studies show that biomarkers Interlukin-6 (IL-6) and Procalcitonin (PCT) correlate to sepsis progression and response to treatment. Read More

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http://dx.doi.org/10.1007/s10544-020-00492-6DOI Listing

3D-Printed micro-optofluidic device for chemical fluids and cells detection.

Biomed Microdevices 2020 May 18;22(2):37. Epub 2020 May 18.

Department of Electrical Electronic and Computer Science Engineering, University of Catania, viale A. Doria 6, 95125, Catania, CT, Italy.

In this work, it is presented a micro-optofluidic flow detector used for on-chip biological and chemical samples investigation. It is made in Poly-dimethyl-siloxane using a master-slave approach based on the 3D-Printing techniques. The micro-optofluidic device is made by assembling a microfluidic T-junction with a micro-optical section that consists of two optical fiber insertions and a PDMS gold-spattered micro-waveguide. Read More

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http://dx.doi.org/10.1007/s10544-020-00487-3DOI Listing

Biomimetic aorta-gonad-Mesonephros-on-a-Chip to study human developmental hematopoiesis.

Biomed Microdevices 2020 May 6;22(2):34. Epub 2020 May 6.

Department of Micro Engineering, Kyoto University, Kyoto Daigaku-Katsura C3, Nishikyo-ku, Kyoto, 615-8540, Japan.

A fundamental limitation in the derivation of hematopoietic stem and progenitor cells is the imprecise understanding of human developmental hematopoiesis. Herein we established a multilayer microfluidic Aorta-Gonad-Mesonephros (AGM)-on-a-chip to emulate developmental hematopoiesis from pluripotent stem cells. The device consists of two layers of microchannels separated by a semipermeable membrane, which allows the co-culture of human hemogenic endothelial (HE) cells and stromal cells in a physiological relevant spatial arrangement to replicate the structure of the AGM. Read More

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http://dx.doi.org/10.1007/s10544-020-00488-2DOI Listing

Optical measurement platform of temperature-dependent interaction between nanoliposomes and a polymer-grafted surface.

Biomed Microdevices 2020 May 4;22(2):33. Epub 2020 May 4.

Department of Materials Engineering, School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-8656, Japan.

In this study, we report an opto-microfluidic platform integrated with dark-field light scattering imaging and fluorescence microspectroscopy to investigate the temperature-dependent behavior of nanoliposomes. This newly developed experimental platform enabled both in situ measurements of the temperature of the sample solution and observation of individual nanoliposomes. As a proof-of-concept, the temperature-dependent interaction between 1,2-dilauroyl-sn-glycero-3-phosphocholine (DLPC) nanoliposomes and a poly(ethylene glycol) (PEG) grafted surface was investigated using this opto-microfluidic platform. Read More

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http://dx.doi.org/10.1007/s10544-020-00489-1DOI Listing

Rapid lipid bilayer membrane formation on Parylene coated apertures to perform ion channel analyses.

Biomed Microdevices 2020 Apr 30;22(2):32. Epub 2020 Apr 30.

Institute for Microsensors, -actuators and -systems (IMSAS), University of Bremen, Microsystems Center Bremen (MCB), Bremen, Germany.

We present a chip design allowing rapid and robust lipid bilayer (LBL) membrane formation using a Parylene coated thin silicon nitride aperture. After bilayer formation, single membrane channels can be reconstituted and characterized by electrophysiology. The ability for robust reconstitution will allow parallelization and enhanced screening of small molecule drugs acting on or permeating across the membrane channel. Read More

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http://dx.doi.org/10.1007/s10544-020-0473-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7192868PMC

Breast Tumor Targeting with PAMAM-PEG-5FU-Tc As a New Therapeutic Nanocomplex: In In-vitro and In-vivo studies.

Biomed Microdevices 2020 Apr 25;22(2):31. Epub 2020 Apr 25.

Department of Nuclear Science and Technology Research Institute, Tehran, Iran.

Dendrimer-based targeted drug delivery, as an innovative polymeric drug-delivery system, is promising for cancer therapy. Folate receptors (FR) are overexpressed in many types of tumor cells, such as breast cell carcinomas, which allow folate-targeted delivery. Therefor polyethylene glycol (PEG) modified-PAMAM G4 dendrimers were functionalized with folic acid (FA), as targeting agent. Read More

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http://dx.doi.org/10.1007/s10544-020-00485-5DOI Listing

Interdigitated aluminium and titanium sensors for assessing epithelial barrier functionality by electric cell-substrate impedance spectroscopy (ECIS).

Biomed Microdevices 2020 Apr 24;22(2):30. Epub 2020 Apr 24.

Laboratory of Radiobiology, University Hospital for Radiotherapy and Radiation Oncology, Medical University of Innsbruck, Anichstraße 35, A-6020, Innsbruck, Austria.

Electric cell-substrate impedance spectroscopy (ECIS) enables non-invasive and continuous read-out of electrical parameters of living tissue. The aim of the current study was to investigate the performance of interdigitated sensors with 50 μm electrode width and 50 μm inter-electrode distance made of gold, aluminium, and titanium for monitoring the barrier properties of epithelial cells in tissue culture. At first, the measurement performance of the photolithographic fabricated sensors was characterized by defined reference electrolytes. Read More

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http://dx.doi.org/10.1007/s10544-020-00486-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7181462PMC

Detection of renal biomarkers in chronic kidney disease using microfluidics: progress, challenges and opportunities.

Biomed Microdevices 2020 Apr 22;22(2):29. Epub 2020 Apr 22.

Medical Devices Research Centre, National Research Council Canada, 75 de Mortagne Boulevard, Boucherville, QC, J4B6Y4, Canada.

Chronic kidney disease (CKD) typically evolves over many years in a latent period without clinical signs, posing key challenges to detection at relatively early stages of the disease. Accurate and timely diagnosis of CKD enable effective management of the disease and may prevent further progression. However, long turn-around times of current testing methods combined with their relatively high cost due to the need for established laboratory infrastructure, specialized instrumentation and trained personnel are drawbacks for efficient assessment and monitoring of CKD, especially in underserved and resource-poor locations. Read More

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http://dx.doi.org/10.1007/s10544-020-00484-6DOI Listing

Hydrodynamic flow cytometer performance enhancement by two-dimensional acoustic focusing.

Biomed Microdevices 2020 03 28;22(2):27. Epub 2020 Mar 28.

Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou, 215163, China.

Conventional flow cytometers employ hydrodynamic focusing method to insure detection accuracy by forcing cells go through detected position. However, an increased flow velocity will significantly reduce detection precision due to a fact that cells will deviate center position and are easily silted in choke point. In an effort to overcome this limitation, a two-dimension ultrasonic particle focusing method are presented in this work to enhance the performance of flow cytometer. Read More

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http://dx.doi.org/10.1007/s10544-020-00481-9DOI Listing
March 2020
2.877 Impact Factor

Highly sensitive vertical flow based point-of-care immunokit for rapid and early detection of human CRP as a cardiovascular risk factor.

Biomed Microdevices 2020 03 28;22(2):28. Epub 2020 Mar 28.

Department of Physical Sciences, Institute of Advanced Research, Gandhinagar, Gujarat, 382426, India.

Early detection and treatment of cardiovascular disease by identifying markers is important to improve the health of our citizens and simultaneously reduce the cost of health care. Therefore, there is an urgent need for early detection and treatment of asymptomatic cardiovascular disease. Herein, we have developed highly sensitive vertical flow immunokit (VFIK) for the detection of C-reactive protein (CRP), comprising an antibody/citrate conjugated gold nanoparticle in a fixed orientation surface. Read More

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http://dx.doi.org/10.1007/s10544-020-00480-wDOI Listing

Development of a wireless accelerometer-based Intravaginal device to detect pelvic floor motion for evaluation of pelvic floor dysfunction.

Biomed Microdevices 2020 03 17;22(2):26. Epub 2020 Mar 17.

Renovia Inc., Boston, MA, USA.

Urinary incontinence (UI) is experienced by an estimated 51% of women in the U.S. and often results from impaired function or weakening of the pelvic floor muscles. Read More

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http://dx.doi.org/10.1007/s10544-020-00479-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7078143PMC

Enhanced chromosome extraction from cells using a pinched flow microfluidic device.

Biomed Microdevices 2020 03 12;22(2):25. Epub 2020 Mar 12.

State of Utah Center of Excellence for Biomedical Microfluidics, University of Utah, Salt Lake City, UT, USA.

Extraction and purification of intact chromosomes are critical sample preparation steps for transchromosomic research and other applications. The commonly used sample preparation methods lead to too few chromosomes with chromosome deactivation and degradation. In this paper, a "mild" chromosome extraction process that combines a chemical and mechanical lysis approach is introduced for the preparation of intact chromosomes that can readily be used for downstream processing. Read More

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http://dx.doi.org/10.1007/s10544-020-0477-7DOI Listing

Biological and antibacterial properties of TiO coatings containing Ca/P/Ag by one-step and two-step methods.

Biomed Microdevices 2020 03 12;22(2):24. Epub 2020 Mar 12.

School of Materials Science and Engineering, Hebei University of Technology, Tianjin, 300130, People's Republic of China.

The porous TiO coatings containing Ca/P/Ag were separately prepared on titanium (Ti) surface by one-step (micro-arc oxidation) and two-step methods (micro-arc oxidation and cathodic deposition), and then their surface morphology, composition, biological and antibacterial properties were compared. The results showed that the porous coatings containing Ca/P/Ag achieved by different methods showed similar surface morphology and elemental composition, however, by one-step method, silver existed in the coating as silver phosphate, while in the coatings prepared by two-step method, silver existed as metallic silver. Although both coatings showed excellent antibacterial property (the antimicrobial rate is over 99. Read More

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http://dx.doi.org/10.1007/s10544-020-00482-8DOI Listing
March 2020
2.877 Impact Factor

Isolation and mutational assessment of pancreatic cancer extracellular vesicles using a microfluidic platform.

Biomed Microdevices 2020 03 11;22(2):23. Epub 2020 Mar 11.

Department of Bioengineering, Rice University, 6566 Main St, Houston, TX, 77030, USA.

Cancer cells release extracellular vesicles known as extracellular vesicles (EVs), containing tumor-derived DNA, RNA and proteins within their cargo, into the circulation. Circulating tumor-derived extracellular vesicles (TEV) can be used in the context of serial "liquid biopsies" for early detection of cancer, for monitoring disease burden in patients, and for assessing recurrence in the post-resection setting. Nonetheless, isolating sufficient TEV by ultracentrifugation-based approaches, in order to enable molecular assessment of EVs cargo, can be an arduous, time-consuming process and is inconsistent in the context of yield and purity among institutions. Read More

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http://dx.doi.org/10.1007/s10544-020-00483-7DOI Listing

Enzymatically functionalized polyaniline thin films produced with one-step electrochemical immobilization and its application in glucose and urea potentiometric biosensors.

Biomed Microdevices 2020 02 27;22(1):22. Epub 2020 Feb 27.

Department of Physics, Faculty of Philosophy, Sciences and Letters at Ribeirão Preto, University of São Paulo - USP, Av. Bandeirantes 3900, Ribeirão Preto, SP, 14040-901, Brazil.

Glucose and urea enzymatic biosensors were fabricated. One-step electrochemical immobilization process was used to produce thin polyaniline films with entrapped enzymes. Chronopotentiometric analysis, scanning electron microscopy, electrochemical impedance spectroscopy and optical reflectance spectroscopy were used to determine the structure-property relationship of the functionalized polymeric thin films. Read More

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http://dx.doi.org/10.1007/s10544-020-00478-4DOI Listing
February 2020

Posture related in-vitro characterization of a flow regulated MEMS CSF valve.

Biomed Microdevices 2020 02 22;22(1):21. Epub 2020 Feb 22.

Product Development Group Zurich, Department of Mechanical and Process Engineering, ETH Zurich, Tannenstrasse 3, CLA G 21.1, 8092, Zurich, Switzerland.

Overdrainage in upright position is one of the most prevalent issues in treating hydrocephalus with a cerebrospinal fluid (CSF) shunt. Anti-siphon devices (ASDs) are employed to reduce this problem. A novel microelectromechanical system (MEMS)-based valve, termed Chronoflow device, aims to regulate CSF drainage indifferently of the body posture. Read More

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http://dx.doi.org/10.1007/s10544-020-0471-0DOI Listing
February 2020

Cell trapping microfluidic chip made of Cyclo olefin polymer enabling two concurrent cell biology experiments with long term durability.

Biomed Microdevices 2020 02 20;22(1):20. Epub 2020 Feb 20.

Department of Chemical Engineering, Biosystems Engineering Laboratory, Bogazici University, 34342, Istanbul, Turkey.

Cyclo Olefin Polymer (COP) based microbioreactors on a microfluidic chip were produced in house by hot-embossing and thermo-compression bonding methods. The chip allows two different experiments to be performed on trapped cells at the same time. On one side of the chip, red fluorescent protein (RFP) tagged nucleolar Nop56 protein was used to track changes in cell cycle as well as protein synthesis within the yeast cells under the application of the anti-tumor agent hydroxyurea (HU). Read More

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http://dx.doi.org/10.1007/s10544-020-0474-xDOI Listing
February 2020

2 factorial design-based biocompatible microneedle arrays containing artemether co-loaded with lumefantrine nanoparticles for transepidermal delivery.

Biomed Microdevices 2020 02 19;22(1):19. Epub 2020 Feb 19.

Shobhaben Pratapbhai Patel School of Pharmacy and Technology Management, SVKM'S NMIMS, V. L. Mehta Road, Vile Parle (W), Mumbai, India.

The present study was intended to enhance the permeation of artemether and lumefantrine by encapsulating in dissolvable microneedle arrays for extended action. Lumefantrine-nanoparticles were synthesized using chitosan mediated gelation and optimized by 2 factorial designs. The particle size, zeta potential and % entrapment efficiency of the optimized nanoparticles F5 were 105 ± 3. Read More

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http://dx.doi.org/10.1007/s10544-020-0476-8DOI Listing
February 2020

A centrifugal microfluidic emulsifier integrated with oil storage structures for robust digital LAMP.

Biomed Microdevices 2020 02 15;22(1):18. Epub 2020 Feb 15.

State Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai, 200050, China.

Centrifugal droplet-based microfluidic devices have been applied to biomedical analysis and diagnostics recently. However, in centrifugal droplet-based microfluidic devices, droplets are tightly packed (i.e. Read More

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http://dx.doi.org/10.1007/s10544-020-0475-9DOI Listing
February 2020
2.877 Impact Factor

Screen-printed electrochemical biosensor based on a ternary Co@MoS/rGO functionalized electrode for high-performance non-enzymatic glucose sensing.

Biomed Microdevices 2020 02 15;22(1):17. Epub 2020 Feb 15.

School of Mechanical Engineering, and Jiangsu Key Laboratory for Design and Manufacture of Micro-Nano Biomedical Instruments, Southeast University, Nanjing, 211189, China.

In this study, cobalt oxides functionalized MoS/reduced graphene oxide was synthesized via a facile one-pot hydrothermal approach. Morphology and crystal structure of this ternary nanoarchitecture were characterized through scanning electron microscopy, transmission electron microscopy, Raman spectra and X-ray photoelectron spectroscopy. An ultrasensitive non-enzymatic glucose sensor was developed by decorating this ternary nanohybrid on the working electrode of a screen-printed electrochemical sensor. Read More

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http://dx.doi.org/10.1007/s10544-020-0472-zDOI Listing
February 2020

Research Progress of nucleic acid delivery vectors for gene therapy.

Biomed Microdevices 2020 01 27;22(1):16. Epub 2020 Jan 27.

Department of Orthopedics, The First Affiliated Hospital of Xi'an Medical University, 48 Feng Hao Eest Road, Xi'an, 710077, China.

Gene therapy has broad prospects as an effective treatment for some cancers and hereditary diseases. However, DNA and siRNA are easily degraded in vivo because of their biological activities as macromolecules, and they need the effective transmembrane delivery carrier Selecting the appropriate carrier for delivery will allow nucleic acid molecules to reach their site of action and enhance delivery efficiency. Currently used nucleic acid delivery vectors can be divided into two major categories: viral and non-viral vectors. Read More

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http://dx.doi.org/10.1007/s10544-020-0469-7DOI Listing
January 2020
2.877 Impact Factor

Enhancing droplet transition capabilities using sloped microfluidic channel geometry for stable droplet operation.

Biomed Microdevices 2020 01 21;22(1):15. Epub 2020 Jan 21.

Department of Biomedical Engineering, Texas A&M University, College Station, TX, 77843, USA.

Droplet-based microfluidics technology allows for the generation and control of droplets that function as independent chemical and biological reactors, enabling broad ranges of high-throughput assays. As more complex multi-step assays are being realized in droplet format, maintaining droplet stability throughout the assay becomes a critical requirement. Unfortunately, as droplets go through multiple manipulation steps, droplet breakage is commonly seen, especially where droplets have to go through sharp transitions in direction and shape. Read More

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http://dx.doi.org/10.1007/s10544-019-0466-xDOI Listing
January 2020

Analysis of the capacitance of minimally insulated parallel wires implanted in biological tissue.

Biomed Microdevices 2020 01 21;22(1):14. Epub 2020 Jan 21.

Department of Biomedical Engineering, The University of Melbourne, Parkville, Australia.

State of the art bioelectronic implants are using thin cables for therapeutic electrical stimulation. If cable insulation is thin, biological tissue surrounding cables can be unintentionally stimulated. The capacitance of the cable must be much less than the stimulating electrodes to ensure stimulating currents are delivered to the electrode-tissue interface. Read More

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http://dx.doi.org/10.1007/s10544-019-0467-9DOI Listing
January 2020

On-chip simultaneous rotation of large-scale cells by acoustically oscillating bubble array.

Biomed Microdevices 2020 01 18;22(1):13. Epub 2020 Jan 18.

State Key Laboratory of Precision Measurement Technology and Instrument, Department of Precision Instrument, Tsinghua University, Beijing, 100084, China.

Bubbles locating in microfluidic chamber can produce acoustic streaming vortices by applying travelling surface acoustic wave oscillation in an ultrasonic range, which can be used to drive bio-samples to move within the flow field. In this paper, a strategy of bubble array configured in a large number of regularly arranged horseshoe structures is proposed to capture and rotate cells simultaneously. By modifying the geometric parameters of the horseshoe structure and microfluidic setting, high bubble homogeneity and cell trapping percentage was achieved. Read More

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http://dx.doi.org/10.1007/s10544-020-0470-1DOI Listing
January 2020

Fabrication of separable microneedles with phase change coating for NIR-triggered transdermal delivery of metformin on diabetic rats.

Biomed Microdevices 2020 01 7;22(1):12. Epub 2020 Jan 7.

Department of Polymer Materials, College of Materials Science and Engineering, Zhejiang Sci-Tech University, Hangzhou, 310018, Zhejiang, China.

To enhance the compliance of drug delivery for patients, the novel near-infrared (NIR) light-triggered and separable microneedles (MNs) have been developed in this work. Firstly, prussian blue nanoparticles (PB NPs) as the photo-thermal conversion factor and metformin as the hypoglycemic drug were embedded into the separable arrowheads, which consisted by poly (vinyl alcohol) and sucrose (PVA/Suc). The arrowheads of MNs were located on soluble solids supporting substrates that produced by poly(vinyl pyrrolidone) (PVP). Read More

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http://dx.doi.org/10.1007/s10544-019-0468-8DOI Listing
January 2020

Development of a new and simple method for the detection of histidine-tagged proteins based on thionine-chitosan/gold nanoparticles/horseradish peroxidase.

Biomed Microdevices 2020 01 2;22(1):11. Epub 2020 Jan 2.

College of Biotechnology & food Science, Tianjin University of Commerce, Tianjin, 300314, China.

In the current study, an electrochemical biosensing signal amplification system was utilized with thionine-chitosan-gold nanoparticles (Chit-GNPs) that absorbed horseradish peroxidase (HRP) and anti-His tagged protein monoclonal antibody derived from Balb/c mice. In addition, transmission electron microscopy (TEM) was used to characterize the nanogold solution and atomic force microscopy (AFM) was used to characterize the sensor assembly. To evaluate the quality of the immunosensor, the amperometric I-t curve method was applied to determine His-IL23 in PBS. Read More

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http://dx.doi.org/10.1007/s10544-019-0464-zDOI Listing
January 2020

Bioprinting a 3D vascular construct for engineering a vessel-on-a-chip.

Biomed Microdevices 2019 12 26;22(1):10. Epub 2019 Dec 26.

Department of Cardiac Surgery, Zhongshan Hospital, Fudan University, Shanghai, 200032, China.

The organ-on-a-chip model mimics the structural and functional features of human tissues or organs and has great importance in translational research. For vessel-on-a-chip model, conventional fabrication techniques are unable to efficiently imitate the intimal-medial unit of the vessel wall. Bioprinting technology, which can precisely control the organization of cells, biomolecules, and the extracellular matrix, has the potential to fabricate three-dimensional (3D) tissue constructs with spatial heterogeneity. Read More

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http://dx.doi.org/10.1007/s10544-019-0460-3DOI Listing
December 2019
2.877 Impact Factor

Microfluidic vascular-bed devices for vascularized 3D tissue engineering: tissue engineering on a chip.

Biomed Microdevices 2019 12 20;22(1). Epub 2019 Dec 20.

Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, 8-1 Kawada-Cho, Shinjuku-Ku, Tokyo, 162-8666, Japan.

In this report, we describe a microfluidic vascular-bed (micro-VB) device providing a platform for 3D tissue engineering with vascular network formation. The micro-VB device allows functional connections between endothelial capillaries of heterogeneous sections (5-100 μm in diameter) and artificial plastic tubes or reservoirs (1-10 mm in diameter). Moreover, the micro-VB device can be installed in a standard 100 mm-diameter Petri dish. Read More

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http://dx.doi.org/10.1007/s10544-019-0461-2DOI Listing
December 2019

Coated microneedles for transdermal delivery of a potent pharmaceutical peptide.

Biomed Microdevices 2019 12 16;22(1). Epub 2019 Dec 16.

Merck & Co., Inc., 2000 Galloping Hill Rd, Kenilworth, NJ, 07033, USA.

Minimally invasive delivery of peptide and protein molecules represents a significant opportunity for product differentiation and value creation versus standard injectable routes of administration. One such technology utilizes microneedle (MN) patches and it has made considerable clinical advances in systemic delivery of potent macromolecules and vaccines. A sub-class of this technology has focused on preparation of solid dense MN arrays followed by precision formulation coating on the tips of the MN. Read More

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http://dx.doi.org/10.1007/s10544-019-0462-1DOI Listing
December 2019

Bio-inspired microneedle design for efficient drug/vaccine coating.

Biomed Microdevices 2019 12 16;22(1). Epub 2019 Dec 16.

Institute of Applied Physics, Johannes Kepler University Linz, 4040, Linz, Austria.

Biomimetics is the interdisciplinary scientific field focused on the study and imitation of biological systems, with the aim of solving complex technological problems. In this paper, we present a new bio-inspired design for microneedles (MNs) and MN arrays, intended for rapidly coating the MNs with drug/vaccine. The biomimetic approach consists in ornamenting the lateral sides of pyramidal MNs with structures inspired by the external scent efferent systems of some European true bugs, which facilitate a directional liquid transport. Read More

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http://dx.doi.org/10.1007/s10544-019-0456-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6915113PMC
December 2019
6 Reads

Paper based point of care immunosensor for the impedimetric detection of cardiac troponin I biomarker.

Biomed Microdevices 2019 12 16;22(1). Epub 2019 Dec 16.

Amity Institute of Nanotechnology, Amity University, Noida, UP, 201301, India.

Advancements in health care monitoring demand a rapid, accurate and reliable early diagnosis of "Heart Attack" (acute myocardial infarction) with an objective to develop a cost-effective, rapid and label-free point of care diagnostic test kit for the detection of cardiac troponin I (cTnI) on paper-based multi-frequency impedimetric transducers. Paper based sensing platforms were developed by integrating carboxyl group functionalized multi-walled carbon nanotubes (MWCNT) with antibodies of cardiac troponin I (anti-cTnI) biomarker and was characterized using Electrochemical Impedance Spectroscopy (EIS). Various concentrations of cTnI with anti cTnI were studied as a function of impedance change. Read More

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http://dx.doi.org/10.1007/s10544-019-0463-0DOI Listing
December 2019
2.877 Impact Factor

Design and fabrication of portable continuous flow PCR microfluidic chip for DNA replication.

Biomed Microdevices 2019 12 10;22(1). Epub 2019 Dec 10.

Photonics and Bio-medical Research Institute, Department of Physics Faculty of Science, East China University of Science and Technology, Shanghai, China.

The reasons for restricting continuous flow polymerase chain reaction (CF-PCR) microfluidic chip from lab to application are that it is not portable and requires costly external precision pumps for sample injection. Herein, we employed water as the substitute for PCR solution, and investigated the effect of the cross-section, width-to-depth ratio, and the length ratio for three temperature zones of the micro channel on the thermal and flow distribution of fluid in micro tube by finite element analysis. Results show that the central velocity is uniform and stable velocity occupies the most if the cross-section is rectangular. Read More

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http://dx.doi.org/10.1007/s10544-019-0457-yDOI Listing
December 2019

Development of rolled scaffold for high-density adherent cell culture.

Biomed Microdevices 2019 12 7;22(1). Epub 2019 Dec 7.

Division of Electrical and Computer Engineering, Louisiana State University, Baton Rouge, LA, 70803, USA.

Bioreactors for large-scale culture of mammalian cells are playing vital roles in biotechnology and bioengineering. Various bioreactors have been developed, but their capacity and efficiency are often limited by insufficient mass transfer rate and high shear stress. A rolled scaffold (RS) is a fully defined scaffold for high-density adherent culture of mammalian cells. Read More

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http://dx.doi.org/10.1007/s10544-019-0459-9DOI Listing
December 2019

An overview on the exponential growth of non-invasive diagnosis of diabetes mellitus from exhaled breath by nanostructured metal oxide Chemi-resistive gas sensors and μ-preconcentrator.

Biomed Microdevices 2019 12 3;22(1). Epub 2019 Dec 3.

Department of Biomedical Engineering, SRM Institute of Science and Technology, Kattankulathur, Tamil Nadu, 603203, India.

The characterization of acetone in exhaled breath reflects the internal metabolism of glucose in bloodstream and airways. This phenomenon provides a great potential for non-invasive diagnosis of diabetes mellitus and has inspired medical sodalities as an alternative diagnostic tool. This review discusses about the origination of acetone in breath, its correlation with blood glucose level along with the ways to collect breath sample. Read More

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http://dx.doi.org/10.1007/s10544-019-0448-zDOI Listing
December 2019

Estimation of pO histogram from a composite EPR Spectrum of multiple random implants.

Biomed Microdevices 2019 12 3;22(1). Epub 2019 Dec 3.

Department of Biomedical Engineering, The Ohio State University, Columbus, OH, 43210, USA.

Electron paramagnetic resonance (EPR) spectroscopy using oxygen-sensing implants can provide reliable and repeated measurements of the partial pressure of oxygen (pO) over a period of months or longer; however, it does not provide accurate information about the distribution of tissue oxygenation. While EPR imaging has the capability to provide spatially resolved oxygen data, it is time-consuming and not optimized for discrete number of implants. Previous reports suggest multi-site algorithms, which would require either the implants to be aligned in a certain way so as to deconvolve them using a linear magnetic field gradient or sparse imaging of the implants from a small number of 3D projections. Read More

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http://dx.doi.org/10.1007/s10544-019-0451-4DOI Listing
December 2019

One-step microchip for DNA fluorescent labeling.

Biomed Microdevices 2019 11 28;22(1). Epub 2019 Nov 28.

Department of Nano-Bio Mechanical System Engineering, College of Engineering, Chonbuk National University, Jeonju, 54896, South Korea.

In this study, we propose a microchip that is sequentially capable of fluorescently staining and washing DNAs. The main advantage of this microchip is that it allows for one-step preparation of small amounts of solution without degrading microscopic bio-objects such as the DNAs, cells, and biomolecules to be stained. The microchip consists of two inlets, the main channel, staining zone, washing zone, and one outlet, and was processed using a femtosecond laser system. Read More

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http://dx.doi.org/10.1007/s10544-019-0454-1DOI Listing
November 2019

Fabrication and characterization of hyaluronic acid microneedles to enhance delivery of magnesium ascorbyl phosphate into skin.

Biomed Microdevices 2019 11 27;21(4):104. Epub 2019 Nov 27.

Department of Pharmaceutical Sciences, College of Pharmacy, Mercer University, Atlanta, GA, 30341, USA.

This study investigated the in vitro transdermal delivery of magnesium ascorbyl phosphate (MAP) through porcine ear skin treated with hyaluronic acid (HA) microneedles (MNs). In this study, the micro-molding method was used to fabricate HA MNs. HA solution (10% w/v) containing 3% of MAP was placed onto a poly(dimethyl siloxane) mold to fill the microchannels under vacuum followed by drying in a desiccator. Read More

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http://dx.doi.org/10.1007/s10544-019-0455-0DOI Listing
November 2019

Soft dry electroophthalmogram electrodes for human machine interaction.

Biomed Microdevices 2019 11 27;21(4):103. Epub 2019 Nov 27.

Rail Transportation Technology Innovation Center, East China Jiaotong University, Nanchang, 330013, China.

Current soft surface electrodes have attracted more and more attentions owing to their potential applications in biological signal monitoring, human-machine interaction (HMI) and Internet of Things (IoT). The paper presents that soft dry electrode based on polydimethylsiloxane-carbon black (PDMS-CB) conductive polymer is designed and fabricated to continuous, long-term, stable electroophthalmogram (EOG) signal recordings for HMI applications. The features corresponding to the different eye motions are extracted from the EOG data via the soft dry electrodes. Read More

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http://dx.doi.org/10.1007/s10544-019-0458-xDOI Listing
November 2019

A theoretical study on real time monitoring of single cell mitosis with micro electrical impedance tomography.

Biomed Microdevices 2019 11 25;21(4):102. Epub 2019 Nov 25.

Department of Mechanical Engineering, University of California Berkeley, Berkeley, CA, 94720, USA.

Real time monitoring of cell division, mitosis, at the single cell level, has value for many biomedical applications; such as developing optimal cancer treatments that target the cell division process. The goal of this theoretical study is to explore the feasibility of using Micro Electrical Impedance Tomography (MEIT) for real time monitoring of mitosis in a single cell, through imaging. MEIT employs a micro (single cell) scale electrode cage with electrodes placed around the cell. Read More

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http://dx.doi.org/10.1007/s10544-019-0452-3DOI Listing
November 2019

Characterizing the invasion of different breast cancer cell lines with distinct E-cadherin status in 3D using a microfluidic system.

Biomed Microdevices 2019 11 23;21(4):101. Epub 2019 Nov 23.

Microsystems group, Department of Mechanical Engineering and Institute for Complex Molecular Systems (ICMS), Eindhoven University of Technology, Groene Loper 15, 5612AZ, Eindhoven, the Netherlands.

E-cadherin is a cell-cell adhesion protein that plays a prominent role in cancer invasion. Inactivation of E-cadherin in breast cancer can arise from gene promoter hypermethylation or genetic mutation. Depending on their E-cadherin status, breast cancer cells adopt different morphologies with distinct invasion modes. Read More

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http://dx.doi.org/10.1007/s10544-019-0450-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6875428PMC
November 2019

Determining the factors affecting dynamic insertion of microneedles into skin.

Biomed Microdevices 2019 11 19;21(4):100. Epub 2019 Nov 19.

Department of Electrical and Computer Engineering, The University of British Columbia, Vancouver, BC, V6T 1Z4, Canada.

Microneedles are extremely small and minimally-invasive intradermal drug delivery devices that require controlled, accurate, and repeatable insertions into human skin to perform their functions. Due to high variability and elasticity of human skin, dynamic insertion methods are being sought to ensure success in microneedle insertions into the skin passed the tough stratum corneum layer. Dynamic microneedle insertions have not been thoroughly studied to identify and assess the key parameters influencing the skin fracture to date. Read More

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http://dx.doi.org/10.1007/s10544-019-0449-yDOI Listing
November 2019

A high-throughput microfluidic method for fabricating aligned collagen fibrils to study Keratocyte behavior.

Biomed Microdevices 2019 11 18;21(4):99. Epub 2019 Nov 18.

Department of Bioengineering, University of Texas at Dallas, 800 W. Campbell Rd, Richardson, TX, 75080, USA.

In vivo, keratocytes are surrounded by aligned type I collagen fibrils that are organized into lamellae. A growing body of literature suggests that the unique topography of the corneal stroma is an important regulator of keratocyte behavior. In this study we describe a microfluidic method to deposit aligned fibrils of type I collagen onto glass coverslips. Read More

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http://dx.doi.org/10.1007/s10544-019-0436-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7228026PMC
November 2019

Engineered topographical structure to control spatial cell density using cell migration.

Biomed Microdevices 2019 11 15;21(4):98. Epub 2019 Nov 15.

Department of Information Physics and Computing, Graduate School of information Science and Technology, The University of Tokyo, Tokyo, 113-8656, Japan.

Control of the spatial distribution of various cell types is required to construct functional tissues. Here, we report a simple topographical structure changed the spatial cell density. A concave curved boundary was designed, which allowed the spatial descent moving of cells and the change in spatial distributions of co-cultured cells. Read More

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http://dx.doi.org/10.1007/s10544-019-0447-0DOI Listing
November 2019

Performance and structural comparison of hydrogels made from wheat bran arabinoxylan using enzymatic and coacervation methods as micro-and nano- encapsulation and delivery devices.

Biomed Microdevices 2019 11 15;21(4):97. Epub 2019 Nov 15.

Department of Processing Engineering, Stellenbosch University, Private Bag XI, Stellenbosch, 7602, South Africa.

This study evaluated the structural and performance differences between arabinoglucuronoxylan micro-hydrogels that were enzymatically produced from alkaline-extracted wheat bran arabinoglucuronoxylans using recombinant α-L-arabinofuranosidase (AbfB) that selectively removes arabinose side chains, and chemically through coacervation process, as delivery devices for bioactive substances. The encapsulations of model bioactive substance, gallic acid (GA), in the hydrogels, were done either in-situ or ex-situ to identify the most effective encapsulation and delivery method. The hydrogels particle size distribution, polydispersity index, GA encapsulation efficiency, retention and release of functional GA (based on antioxidant activity) were assessed. Read More

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http://dx.doi.org/10.1007/s10544-019-0445-2DOI Listing
November 2019

Isothermal titration calorimetry in a 3D-printed microdevice.

Biomed Microdevices 2019 11 11;21(4):96. Epub 2019 Nov 11.

Department of Mechanical Engineering, Columbia University, New York, NY, USA.

Isothermal titration calorimetry (ITC) can benefit from operating in miniaturized devices as they enable quantitative, low-cost measurements with reduced analysis time and reagents consumption. However, most of the existing devices that offer ITC capabilities either do not yet allow proper control of reaction conditions or are limited by issues such as evaporation or surface adsorption caused inaccurate solution concentration information and unintended changes in biomolecular properties because of aggregation. In this paper, we present a microdevice that combines 3D-printed microfluidic structures with a polymer-based MEMS thermoelectric sensor to enable quantitative ITC measurements of biomolecular interactions. Read More

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http://dx.doi.org/10.1007/s10544-019-0444-3DOI Listing
November 2019

Simultaneous and high sensitive detection of Salmonella typhi and Salmonella paratyphi a in human clinical blood samples using an affordable and portable device.

Biomed Microdevices 2019 11 9;21(4):95. Epub 2019 Nov 9.

Centre for Biomedical Engineering, Indian Institute of Technology Delhi, New Delhi, 110016, India.

Enteric fever is one of the leading causes of infection and subsequent fatality (greater than 1.8 million) (WHO 2018), especially in the developing countries due to contaminated water and food inter twinned with unhygienic practices. Clinical gold standard technique of culture-based method followed by biochemical tests demand 72+ hours for diagnosis while newly developed techniques (like PCR, RT-PCR, DNA microarray etc. Read More

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http://dx.doi.org/10.1007/s10544-019-0441-6DOI Listing
November 2019

Semi-permanent transcorneal filter support and in vivo surgical implantation technique for open-angle glaucoma treatment.

Biomed Microdevices 2019 11 4;21(4):92. Epub 2019 Nov 4.

Department of Biomedical Engineering, Purdue University, 206 S Martin Jischke Drive, West Lafayette, IN, 47907, USA.

Primary open-angle glaucoma is a progressive disease affecting nearly 60 million people worldwide which, if left untreated, can lead to optic nerve head damage and complete loss of sight. Current interventions include: pharmaceutical drops, laser surgery, shunts, and bleb; however, these methods provide insufficient long-term efficacy in intraocular pressure management. We developed a semi-permanent, implantable transcorneal duct as a new aid in the treatment of this disease. Read More

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http://dx.doi.org/10.1007/s10544-019-0440-7DOI Listing
November 2019