312 results match your criteria Biomarker insights[Journal]


Identification of Urinary CD44 and Prosaposin as Specific Biomarkers of Urinary Tract Infections in Children With Neurogenic Bladders.

Biomark Insights 2019 15;14:1177271919835570. Epub 2019 Mar 15.

Department of Pediatrics, Cincinnati Children's Hospital Medical Center, Cincinnati, OH, USA.

Purpose: Distinguishing urinary tract infection (UTI) from urinary tract colonization (UTC) in children with neurogenic bladders who require clean intermittent catheterization (CIC) is challenging. Our objective was to identify urinary proteins to distinguish UTI from UTC in CIC-dependent children that have potential to serve as objective markers of UTI.

Experimental Design: A total of 10 CIC-dependent children were included in the mass spectrometry analysis (UTI = 5, UTC = 5). Read More

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http://dx.doi.org/10.1177/1177271919835570DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6421595PMC
March 2019
1 Read

C677T Gene Polymorphism as Risk Factor for Psoriasis in Saudis.

Biomark Insights 2019 24;14:1177271919830973. Epub 2019 Feb 24.

Scientific Research Center, Medical Services Department of Armed Forces, Riyadh, Saudi Arabia.

has been linked with the etiopathogenesis of psoriasis with inconsistent results. C677T polymorphism was evaluated in 106 Saudi psoriasis vulgaris patients and 280 matched healthy controls using PCR-RFLP (restriction fragment length plymorphism) technique. The cardiovascular risk factors were also compared in cases and controls. Read More

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http://dx.doi.org/10.1177/1177271919830973DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6390219PMC
February 2019
3 Reads

The Possibility of Systematic Research Fraud Targeting Under-Studied Human Genes: Causes, Consequences, and Potential Solutions.

Biomark Insights 2019 5;14:1177271919829162. Epub 2019 Feb 5.

Univ Grenoble Alpes, CNRS, Grenoble INP, LIG, Grenoble, France.

A major reason for biomarker failure is the selection of candidate biomarkers based on inaccurate or incorrect published results. Incorrect research results leading to the selection of unproductive biomarker candidates are largely considered to stem from unintentional research errors. The additional possibility that biomarker research may be actively misdirected by research fraud has been given comparatively little consideration. Read More

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http://dx.doi.org/10.1177/1177271919829162DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6366001PMC
February 2019
8 Reads

Technical and Regulatory Considerations for Taking Liquid Biopsy to the Clinic: Validation of the JAX PlasmaMonitor Assay.

Biomark Insights 2019 1;14:1177271919826545. Epub 2019 Feb 1.

The Jackson Laboratory for Genomic Medicine, Farmington, CT, USA.

The standard of care in oncology has been genomic profiling of tumor tissue biopsies for the treatment and management of disease, which can prove to be quite challenging in terms of cost, invasiveness of procedure, and potential risk for the patient. As the number of available drugs in oncology continues to increase, so too does the demand for technologies and testing applications that can identify genomic alterations targetable by these new therapies. Liquid biopsies that use a blood draw from the diseased patient may offset the many disadvantages of the invasive procedure. Read More

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http://dx.doi.org/10.1177/1177271919826545DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6360476PMC
February 2019
5 Reads

The Fraction Exhaled Nitric Oxide as a Biomarker of Asthma Control.

Biomark Insights 2019 31;14:1177271919826550. Epub 2019 Jan 31.

Faculty of Medicine, Universidade Federal de Goias (UFG), Goiânia, Brazil.

Introduction And Objective: The main goal of asthma treatment is to achieve and maintain clinical control of the disease. The exhaled fraction nitric oxide (FeNO) level is a biomarker of T-helper cell type 2 (Th2) inflammation of the airways. Our objective was to determine whether the FeNO level can be used to discriminate between patients with controlled, partially controlled, and uncontrolled asthma. Read More

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http://dx.doi.org/10.1177/1177271919826550DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6357290PMC
January 2019
6 Reads

Peripheral Biomarker for Vascular Disorders.

Biomark Insights 2018 29;13:1177271918812467. Epub 2018 Nov 29.

School of Life Sciences and Centre for Health Technologies, University of Technology Sydney, Broadway, NSW, Australia.

Atherosclerosis is the underlying cause of most myocardial infarction (MI) and ischaemic stroke episodes. An early sign of atherosclerosis is hypertrophy of the arterial wall. It is known that increased intima media thickness (IMT) is a non-invasive marker of arterial wall alteration, which can easily be assessed in the carotid arteries by high-resolution B-mode ultrasound. Read More

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http://dx.doi.org/10.1177/1177271918812467DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6287300PMC
November 2018
19 Reads

Integration of Biomarkers Into a Signature Profile of Persistent Traumatic Brain Injury Involving Autoimmune Processes Following Water Hammer Injury From Repetitive Head Impacts.

Biomark Insights 2018 29;13:1177271918808216. Epub 2018 Oct 29.

Department of Psychology, The University of Texas at Austin and Austin Radiological Association, Austin, TX, USA.

Objectives: To assemble an algorithm that will describe a "Signature" predictive of an individual's vulnerability to persistent traumatic brain injury (TBI).

Subjects And Methods: Studies of athletes and warriors who are subjected to repeated head impacts with rapid acceleration/deceleration forces are used to assist in the diagnosis and management of TBI-affected individuals. Data from multiple areas, including clinical, anatomical, magnetic resonance imaging, cognitive function, and biochemical analyses, are integrated to provide a Signature of persistent TBI. Read More

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http://journals.sagepub.com/doi/10.1177/1177271918808216
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http://dx.doi.org/10.1177/1177271918808216DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6207974PMC
October 2018
14 Reads

MicroRNAs as Biomarkers of B-cell Lymphoma.

Biomark Insights 2018 16;13:1177271918806840. Epub 2018 Oct 16.

Molecular Oncology Group, Biodonostia Research Institute, San Sebastián, Spain.

B-cell lymphomas represent a diverse group of neoplasms classified primarily by histopatholgy and are often challenging to accurately diagnose. Despite having been recognized less than 20 years ago, microRNAs (miRNAs) have emerged as one of the most promising class of cancer molecular biomarkers and are particularly attractive as they can be readily detected in formalin-fixed paraffin-embedded biopsy material and biological fluids such as blood. Many of the identified B-cell lymphoma miRNA biomarkers also play crucial regulatory roles in normal B-cell development. Read More

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http://dx.doi.org/10.1177/1177271918806840DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6195009PMC
October 2018
15 Reads

Identifying Reliable Diagnostic/Predictive Biomarkers for Rheumatoid Arthritis.

Biomark Insights 2018 24;13:1177271918801005. Epub 2018 Sep 24.

School of Pharmacy and Biomedical Sciences, University of Central Lancashire, Preston, UK.

Introduction And Objective: Elevated C-reactive protein is usually a good indicator of rheumatoid arthritis (RA); however, there are limitations that compromise its specificity and therefore there is an urgent need to identify more reliable diagnostic biomarkers to detect early stages of RA. In addition, identifying the correct therapeutic biomarker for the treatment of RA using methotrexate (MTX) would greatly increase the benefits experienced by the patients.

Materials And Methods: Primary normal synoviocytes human fibroblast-like synoviocytes (HFLS) and its phenotype rheumatic HFLS-RA cells were chosen for this study. Read More

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http://dx.doi.org/10.1177/1177271918801005DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6153528PMC
September 2018
4 Reads

Cytokine Measurements for Diagnosing and Characterizing Leukemoid Reactions and Immunohistochemical Validation of a Granulocyte Colony-Stimulating Factor and CXCL8-Producing Renal Cell Carcinoma.

Biomark Insights 2018 17;13:1177271918792246. Epub 2018 Aug 17.

Department of Urology, Faculty of Medicine and Health, Örebro University, Örebro, Sweden.

Background: Various paraneoplastic syndromes are encountered in renal cell carcinomas. This case report illustrates that a paraneoplastic leukemoid reaction may precede the diagnosis of renal cell carcinoma and be explained by cytokine production from the cancer cells.

Case Presentations: A 64-year-old man was referred for hematology workup due to pronounced leukocytosis. Read More

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http://dx.doi.org/10.1177/1177271918792246DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6100120PMC
August 2018
3 Reads

Is Procalcitonin Useful in Pediatric Critical Care Patients?

Biomark Insights 2018 7;13:1177271918792244. Epub 2018 Aug 7.

Pediatric Intensive Care Unit, Paediatric Infectious Diseases Research Group, Institut de Recerca Sant Joan de Déu, CIBERESP, Hospital Sant Joan de Déu Barcelona, Barcelona, Spain.

This review examines the use of procalcitonin in different clinical situations in the pediatric patient, with special emphasis on those requiring intensive care. We review the latest articles on its potency as a biomarker in both infectious processes at diagnosis and on the response to treatment. Read More

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http://dx.doi.org/10.1177/1177271918792244DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6081751PMC
August 2018
2 Reads

Development of a Novel Proteomic Risk-Classifier for Prognostication of Patients With Early-Stage Hormone Receptor-Positive Breast Cancer.

Biomark Insights 2018 30;13:1177271918789100. Epub 2018 Jul 30.

OncoStem Diagnostics, Bangalore, India.

Use of proteomic strategies to identify a risk classifier that estimates probability of distant recurrence in early-stage hormone receptor (HR)-positive breast cancer is relevant to physiological cellular function and therefore to intrinsic tumor biology. We used a 298-sample retrospective training set to develop an immunohistochemistry-based novel risk classifier called CanAssist-Breast (CAB) which combines 5 prognostically relevant biomarkers and 3 clinico-pathological parameters to arrive at probability of distant recurrence within 5 years from diagnosis. Five selected biomarkers, namely, CD44, ABCC4, ABCC11, N-cadherin, and pan-cadherin, were chosen based on their role in tumor metastasis. Read More

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http://dx.doi.org/10.1177/1177271918789100DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6066801PMC
July 2018
7 Reads

Heart Rate Variability as a Biomarker for Predicting Stroke, Post-stroke Complications and Functionality.

Biomark Insights 2018 18;13:1177271918786931. Epub 2018 Jul 18.

Neuroscience Research Unit, School of Life Sciences, University of Technology Sydney, Broadway, NSW, Australia.

Background: Heart rate variability (HRV) is a non-invasive measure of the function of the autonomic nervous system, and its dynamic nature may provide a means through which stroke and its associated complications may be predicted, monitored, and managed.

Objective: The objective of this review is to identify and provide a critique on the most recent uses of HRV in stroke diagnosis/management and highlight areas that warrant further research.

Methods: The MEDLINE, CINAHL, and OVID MEDLINE databases were canvassed using a systematic search strategy, for articles investigating the use of HRV in stroke diagnosis and management. Read More

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http://dx.doi.org/10.1177/1177271918786931DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6052496PMC
July 2018
4 Reads

Nattokinase: A Promising Alternative in Prevention and Treatment of Cardiovascular Diseases.

Biomark Insights 2018 5;13:1177271918785130. Epub 2018 Jul 5.

School of Life Sciences, University of Technology Sydney, Broadway, NSW, Australia.

Cardiovascular disease (CVD) is the leading cause of death in the world and our approach to the control and management of CVD mortality is limited. Nattokinase (NK), the most active ingredient of natto, possesses a variety of favourable cardiovascular effects and the consumption of Natto has been linked to a reduction in CVD mortality. Recent research has demonstrated that NK has potent fibrinolytic activity, antihypertensive, anti-atherosclerotic, and lipid-lowering, antiplatelet, and neuroprotective effects. Read More

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http://dx.doi.org/10.1177/1177271918785130DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6043915PMC
July 2018
28 Reads

Multiple Endocrine Neoplasia Syndromes from Genetic and Epigenetic Perspectives.

Biomark Insights 2018 2;13:1177271918785129. Epub 2018 Jul 2.

Chronic Diseases Research Center, Endocrinology and Metabolism Research Institute, Tehran University of Medical Sciences, Tehran, Iran.

Multiple endocrine neoplasia (MEN) syndromes are infrequent inherited disorders in which more than one endocrine glands develop noncancerous (benign) or cancerous (malignant) tumors or grow excessively without forming tumors. There are 3 famous and well-known forms of MEN syndromes (MEN 1, MEN 2A, and MEN 2B) and a newly documented one (MEN4). These syndromes are infrequent and occurred in all ages and both men and women. Read More

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http://dx.doi.org/10.1177/1177271918785129DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6043927PMC
July 2018
16 Reads

High Frequency of Extractable Nuclear Autoantibodies in Wheat-Related Disorders.

Biomark Insights 2018 18;13:1177271918782893. Epub 2018 Jun 18.

Vibrant Sciences LLC, San Carlos, CA, USA.

Background And Aims: There has been broad interest to explore the presence of autoimmunity among wheat-sensitive individuals, but neither the pathogenesis nor the relevance has been established. In this study, we evaluated the frequencies and levels of autoantibodies, which are important biomarkers of autoimmunity, in subjects with wheat-related disorders and controls. Anti-nuclear antibodies (ANA) and the specific ones against extractable nuclear antigens (ENA) were investigated. Read More

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http://dx.doi.org/10.1177/1177271918782893DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6024268PMC
June 2018
25 Reads

Cytokine Gene Polymorphisms in Saudi Patients With Atopic Dermatitis: A Case-Control Study.

Biomark Insights 2018 4;13:1177271918777760. Epub 2018 Jun 4.

Scientific Research Center, Prince Sultan Military Medical City, Riyadh, Saudi Arabia.

The cause of atopic dermatitis (AD) is multifactorial and a number of genes including cytokines have been involved. We genotyped 315 subjects for polymorphisms in and and genes. Patients had significantly higher frequency of GA genotype of TNF-α (-308 G/A) than healthy controls. Read More

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http://dx.doi.org/10.1177/1177271918777760DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5989047PMC
June 2018
8 Reads

Impaired Cerebral Perfusion in Multiple Sclerosis: Relevance of Endothelial Factors.

Biomark Insights 2018 18;13:1177271918774800. Epub 2018 May 18.

Unit of Neurology and Clinical Neurophysiology, Department of Neurological and Neurosensory Sciences, University Hospital of Siena, Siena, Italy.

Magnetic resonance imaging techniques measuring in vivo brain perfusion and integrity of the blood-brain barrier have developed rapidly in the past decade, resulting in a wide range of available methods. This review first discusses their principles, possible pitfalls, and potential for quantification and outlines clinical application in neurological disorders. Then, we focus on the endothelial cells of the blood-brain barrier, pointing out their contribution in regulating vascular tone by production of vasoactive substances. Read More

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http://dx.doi.org/10.1177/1177271918774800DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5960845PMC
May 2018
5 Reads

The Therapeutic Potential of Blocking Galectin-3 Expression in Acute Myocardial Infarction and Mitigating Inflammation of Infarct Region: A Clinical Outcome-Based Translational Study.

Biomark Insights 2018 3;13:1177271918771969. Epub 2018 May 3.

Division of Cardiovascular Medicine, Department of Medicine, University at Buffalo, Buffalo, NY, USA.

Introduction: Increased galectin-3 is associated with ischemic cardiomyopathy, although its role in early remodeling post-myocardial infarction (MI) has not been fully elucidated. There are no data demonstrating that blocking galectin-3 expression would have an impact on the heart and that its relationship to remodeling is not simply an epiphenomenon. The direct association between galectin-3 and myocardial inflammation, dysfunction, and adverse cardiovascular outcomes post-MI was examined using clinical and translational studies. Read More

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http://dx.doi.org/10.1177/1177271918771969DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5946633PMC
May 2018
12 Reads

Lipidomic Profiling of Plasma and Erythrocytes From Septic Patients Reveals Potential Biomarker Candidates.

Biomark Insights 2018 26;13:1177271918765137. Epub 2018 Mar 26.

Laboratory of Multidisciplinary Research, Sao Francisco University, USF, Sao Francisco de Assis Avenue, Brazil.

Background: Sepsis remains the primary cause of death from infection, despite advances in modern medicine. The identification of reliable diagnostic biomarkers for the early detection of this disease is critical and may reduce the mortality rate as it could allow early treatment. The purpose of this study was to describe the changes in the plasma and red cells blood lipidome profiling of patients diagnosed with sepsis and septic shock with the aim to identify potentially useful metabolic markers. Read More

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http://dx.doi.org/10.1177/1177271918765137DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5882049PMC
March 2018
12 Reads

Technical Validation of a Reverse-Transcription Quantitative Polymerase Chain Reaction In Vitro Diagnostic Test for the Determination of MiR-31-3p Expression Levels in Formalin-Fixed Paraffin-Embedded Metastatic Colorectal Cancer Tumor Specimens.

Biomark Insights 2018 16;13:1177271918763357. Epub 2018 Mar 16.

IntegraGen SA, Evry, France.

MiR-31-3p expression has been shown to be a predictive biomarker for response to anti-epithelial growth factor receptor therapy in patients with RAS wild-type metastatic colorectal cancer (mCRC). To aid in the quantification of miR-31-3p expression in formalin-fixed paraffin-embedded (FFPE) primary tumor samples from patients with mCRC, a reverse-transcription quantitative polymerase chain reaction (RT-qPCR) assay was developed and validated. Assay development included the identification of a microRNA reference standard and the determination of an appropriate relative quantification cutoff for differentiating low versus high miR-31-3p expression. Read More

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http://dx.doi.org/10.1177/1177271918763357DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5858679PMC
March 2018
10 Reads

A Homogeneous Time-Resolved Fluorescence Immunoassay Method for the Measurement of Compound W.

Biomark Insights 2018 6;13:1177271918757484. Epub 2018 Feb 6.

Division of Nuclear Medicine and Medical Services, Department of Radiological Sciences, School of Medicine, UC Irvine Medical Center and VA Long Beach Healthcare System, Long Beach, CA, USA.

Objective: Using compound W (a 3,3'-diiodothyronine sulfate [TS] immuno-crossreactive material)-specific polyclonal antibodies and homogeneous time-resolved fluorescence immunoassay assay techniques (AlphaLISA) to establish an indirect competitive compound W (ICW) quantitative detection method.

Method: Photosensitive particles (donor beads) coated with compound W or TS and rabbit anti-W antibody were incubated with biotinylated goat anti-rabbit antibody. This constitutes a detection system with streptavidin-coated acceptor particle. Read More

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http://dx.doi.org/10.1177/1177271918757484DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5808953PMC
February 2018
8 Reads

Nkx2-3-A Slippery Slope From Development Through Inflammation Toward Hematopoietic Malignancies.

Biomark Insights 2018 6;13:1177271918757480. Epub 2018 Feb 6.

Department of Immunology and Biotechnology, Medical School, University of Pécs, Pécs, Hungary.

The development of peripheral lymphoid tissues from the mesoderm is the result of a complex convergence combining lymphohematopoietic differentiation with the local specification of nonhematopoietic mesenchymal components. Although the various transcriptional regulators with fate-determining effects in diversifying the mobile leukocyte subsets have been thoroughly studied and identified, the tissue-specific determinants promoting the regional differentiation of resident mesenchyme are less understood. Of these factors, various members of the NK-class Nkx paralogues have emerged as key regulators for the organogenesis of spleen and mucosal lymphoid tissues, and recent data have also indicated their involvement in various pathological events, including gut inflammation and hematopoietic malignancies. Read More

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http://dx.doi.org/10.1177/1177271918757480DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5808962PMC
February 2018
7 Reads

Prion Proteins Without the Glycophosphatidylinositol Anchor: Potential Biomarkers in Neurodegenerative Diseases.

Biomark Insights 2018 6;13:1177271918756648. Epub 2018 Feb 6.

Department for the Production of Diagnostic Reagents and Research, Blood Transfusion Centre of Slovenia, Ljubljana, Slovenia.

Prion protein (PrP) is a biomolecule that is involved in neuronal signaling, myelinization, and the development of neurodegenerative diseases. In the cell, PrP is shed by the ADAM10 protease. This process generates PrP molecules that lack glycophosphatidylinositol anchor, and these molecules incorporate into toxic aggregates and neutralize toxic oligomers. Read More

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http://dx.doi.org/10.1177/1177271918756648DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5808966PMC
February 2018
8 Reads

Reactive Oxygen Species: A Key Constituent in Cancer Survival.

Biomark Insights 2018 6;13:1177271918755391. Epub 2018 Feb 6.

Cancer Biology Lab, Department of Biochemistry, GIS, GITAM (Deemed to be University), Visakhapatnam, India.

Background: Cancer is one of the major heterogeneous disease with high morbidity and mortality with poor prognosis. Elevated levels of reactive oxygen species (ROS), alteration in redox balance, and deregulated redox signaling are common hallmarks of cancer progression and resistance to treatment. Mitochondria contribute mainly in the generation of ROS during oxidative phosphorylation. Read More

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http://dx.doi.org/10.1177/1177271918755391DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5808965PMC
February 2018
54 Reads

Evaluation of a Serum Lung Cancer Biomarker Panel.

Biomark Insights 2018 15;13:1177271917751608. Epub 2018 Jan 15.

20/20 GeneSystems, Rockville, MD, USA.

Background: A panel of 3 serum proteins and 1 autoantibody has been developed to assist with the detection of lung cancer. We aimed to validate the accuracy of the biomarker panel in an independent test set and explore the impact of adding a fourth serum protein to the panel, as well as the impact of combining molecular and clinical variables.

Methods: The training set of serum samples was purchased from commercially available biorepositories. Read More

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http://journals.sagepub.com/doi/10.1177/1177271917751608
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http://dx.doi.org/10.1177/1177271917751608DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5772503PMC
January 2018
11 Reads

Kinetics of Procalcitonin in Pediatric Patients on Extracorporeal Membrane Oxygenation.

Biomark Insights 2018 8;13:1177271917751900. Epub 2018 Jan 8.

Pediatric Intensive Care Unit, Paediatric Infectious Diseases Research Group, Institut Recerca Hospital Sant Joan de Déu, Hospital Sant Joan de Déu, CIBERESP, Barcelona, Spain.

Objectives: To assess the kinetics of procalcitonin (PCT) and C-reactive protein (CRP) in pediatric patients who required extracorporeal membrane oxygenation (ECMO) and to analyze its relationship with morbidity and mortality.

Patients And Methods: Prospective observational study including pediatric patients who required ECMO. Both PCT and CRP were sequentially drawn before ECMO (P0) and until 72 hours after ECMO. Read More

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http://dx.doi.org/10.1177/1177271917751900DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5764148PMC
January 2018
22 Reads

Discovery and Longitudinal Evaluation of Candidate Biomarkers for Ischaemic Stroke by Mass Spectrometry-Based Proteomics.

Biomark Insights 2017 20;12:1177271917749216. Epub 2017 Dec 20.

Stroke Department, The Florey Institute of Neuroscience & Mental Health and Melbourne Brain Centre, Melbourne, VIC, Australia.

Application of acute therapies such as thrombolysis for ischaemic stroke (IS) is constrained because of diagnostic uncertainty and the dynamic nature of stroke biology. To investigate changes in blood proteins after stroke and as a result of thrombolysis treatment we performed label-free quantitative proteomics on serum samples using high-resolution mass spectrometry and long high-performance liquid chromatography gradient (5 hours) combined with a 50-cm column to optimise the peptide separation. We identified (false discovery rate [FDR]: 1%) and quantified a total of 574 protein groups from a total of 92 samples from 30 patients. Read More

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http://dx.doi.org/10.1177/1177271917749216DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5751906PMC
December 2017
14 Reads

Plasma Cytochrome c Detection Using a Highly Sensitive Electrochemiluminescence Enzyme-Linked Immunosorbent Assay.

Biomark Insights 2017 13;12:1177271917746972. Epub 2017 Dec 13.

Resuscitation Institute, Rosalind Franklin University of Medicine and Science, North Chicago, IL, USA.

Background: Cytochrome c is an intermembrane mitochondrial protein that is released to the bloodstream following mitochondrial injury.

Methods And Results: We developed an electrochemiluminescence immunoassay to measure cytochrome c in human and rat plasma, which showed high sensitivity with broad dynamic range (2-1200 ng/mL in humans and 5-500 ng/mL in rat) and high assay reproducibility (inter-assay coefficient <6% in humans and <10% in rat). In patients after blunt trauma, plasma cytochrome c directly correlated with injury severity. Read More

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http://dx.doi.org/10.1177/1177271917746972DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5731609PMC
December 2017
14 Reads

Inhaled Steroids and Active Smoking Drive Chronic Obstructive Pulmonary Disease Symptoms and Biomarkers to a Greater Degree Than Airflow Limitation.

Biomark Insights 2017 7;12:1177271917730306. Epub 2017 Sep 7.

Immunology, Janssen Research & Development, LLC, Spring House, PA, USA.

Rationale: Chronic obstructive pulmonary disease (COPD) is a heterogeneous disease, and development of novel therapeutics requires an understanding of pathophysiologic phenotypes.

Objectives: The purpose of the Airways Disease Endotyping for Personalized Therapeutics (ADEPT) study was to correlate clinical features and biomarkers with molecular characteristics in a well-profiled COPD cohort.

Methods: A total of 67 COPD subjects (forced expiratory volume in the first second of expiration [FEV]: 45%-80% predicted) and 63 healthy smoking and nonsmoking controls underwent multiple assessments including patient questionnaires, lung function, and clinical biomarkers including fractional exhaled nitric oxide (FENO), induced sputum, and blood. Read More

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http://dx.doi.org/10.1177/1177271917730306DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5593220PMC
September 2017
22 Reads

Changes in Alternative Splicing as Pharmacodynamic Markers for Sudemycin D6.

Biomark Insights 2017 12;12:1177271917730557. Epub 2017 Sep 12.

University of Kentucky, Lexington, KY, USA.

Objective: The aim of the study was to define pharmacodynamic markers for sudemycin D6, an experimental cancer drug that changes alternative splicing in human blood.

Methods: Blood samples from 12 donors were incubated with sudemycin D6 for up to 24 hours, and at several time points total RNA from lymphocytes was prepared and the pre-messenger RNA (mRNA) splicing patterns were analyzed with reverse transcription-polymerase chain reaction.

Results: Similar to immortalized cells, blood lymphocytes change alternative splicing due to sudemycin D6 treatment. Read More

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http://dx.doi.org/10.1177/1177271917730557DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5598794PMC
September 2017
77 Reads

Chondroitin Sulfate Inhibits Monocyte Chemoattractant Protein-1 Release From 3T3-L1 Adipocytes: A New Treatment Opportunity for Obesity-Related Inflammation?

Biomark Insights 2017 24;12:1177271917726964. Epub 2017 Aug 24.

Duke Molecular Physiology Institute, Duke University School of Medicine, Durham, NC, USA.

Monocyte chemoattractant protein-1 (MCP-1) overproduction from inflamed adipose tissue is a major contributor to obesity-related metabolic syndromes. 3T3-L1 embryonic fibroblasts were cultured and differentiated into adipocytes using an established protocol. Adipocytes were treated with lipopolysaccharide (LPS) to induce inflammation and thus MCP-1 release. Read More

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http://journals.sagepub.com/doi/10.1177/1177271917726964
Publisher Site
http://dx.doi.org/10.1177/1177271917726964DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5574472PMC
August 2017
11 Reads

Prognostic Impact of Urokinase Plasminogen Activator Receptor Expression in Pancreatic Cancer: Malignant Versus Stromal Cells.

Biomark Insights 2017 22;12:1177271917715443. Epub 2017 Jun 22.

Department of Surgery, Leiden University Medical Centre, Leiden, The Netherlands.

The urokinase plasminogen activator receptor (uPAR) has been proposed as a potential prognostic factor for various malignancies. The aim of this study is to assess the prognostic value of uPAR expression in neoplastic and stromal cells of patients with pancreatic adenocarcinoma. Urokinase plasminogen activator receptor expression was determined by immunohistochemistry in 122 pancreatic ductal adenocarcinomas. Read More

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http://dx.doi.org/10.1177/1177271917715443DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5484551PMC
June 2017
17 Reads

Making Meaningful Clinical Use of Biomarkers.

Biomark Insights 2017 19;12:1177271917715236. Epub 2017 Jun 19.

Division of Biochemistry, Department of Basic Sciences and Center for Health Disparities & Molecular Medicine, Loma Linda University Medical Center, Loma Linda, CA, USA.

This review discusses the current state of biomarker discovery for the purposes of diagnostics and therapeutic monitoring. We underscore relevant challenges that have defined the gap between biomarker discovery and meaningful clinical use. We highlight recent advancements in and propose a way to think about future biomarker development. Read More

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http://dx.doi.org/10.1177/1177271917715236DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5479428PMC
June 2017
31 Reads

Influence of Circulating Endothelin-1 and Asymmetric Dimethylarginine on Whole Brain Circulation Time in Multiple Sclerosis.

Biomark Insights 2017 6;12:1177271917712514. Epub 2017 Jun 6.

Unit of Neurology and Neurophysiology, Department of Neurological and Neurosensorial Sciences, University of Siena, Siena, Italy.

Blood-brain barrier (BBB) breakdown, inflammatory and immune cell activation, and chronic cerebral hypoperfusion are features of multiple sclerosis (MS). The aim is to determine the influence of endothelin-1 (ET1) and asymmetric dimethylarginine (ADMA) on cerebral circulation time (CCT) in patients with MS. In all, 64 patients with MS (39 relapsing-remitting [RR]-MS; 25 secondary progressive [SP]-MS subtype) and 37 controls (C) were studied. Read More

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http://dx.doi.org/10.1177/1177271917712514DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5462479PMC
June 2017
29 Reads

Data-Independent Acquisition and Parallel Reaction Monitoring Mass Spectrometry Identification of Serum Biomarkers for Ovarian Cancer.

Biomark Insights 2017 5;12:1177271917710948. Epub 2017 Jun 5.

Department of Molecular Biophysics and Biochemistry, Yale School of Medicine, Yale University, New Haven, CT, USA.

A data-independent acquisition (DIA)/parallel reaction monitoring (PRM) workflow was implemented to identify improved ovarian cancer biomarkers. Data-independent acquisition on ovarian cancer versus control sera and literature searches identified 50 biomarkers and indicated that apolipoprotein A-IV (ApoA-IV) is the most significantly differentially regulated protein. Parallel reaction monitoring with Targeted Ovarian Cancer Proteome Assay validated differential ApoA-IV expression and quantified 9 other biomarkers. Read More

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http://dx.doi.org/10.1177/1177271917710948DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5462478PMC
June 2017
20 Reads

Effects of Acute Exercise on Circulating Soluble Form of the Urokinase Receptor in Patients With Major Depressive Disorder.

Biomark Insights 2017 12;12:1177271917704193. Epub 2017 Apr 12.

Department of Clinical Sciences, Division of Psychiatry, Lund University, Lund, Sweden.

Inflammation has been proposed to play a role in the generation of depressive symptoms. Previously, we demonstrated that patients with major depressive disorder (MDD) have increased plasma levels of the soluble form of the urokinase receptor (suPAR), a marker for low-grade inflammation. The aim of this study was to test the hypothesis that acute exercise would induce inflammatory response characterized by increased suPAR and elucidate whether patients with MDD display altered levels of suPAR in response to acute exercise. Read More

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http://dx.doi.org/10.1177/1177271917704193DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5397280PMC
April 2017
11 Reads

Integrative Analysis of MicroRNA-Mediated Gene Signatures and Pathways Modulating White Blood Cell Count in Childhood Acute Lymphoblastic Leukemia.

Biomark Insights 2017 12;12:1177271917702895. Epub 2017 Apr 12.

Department of Genetics, Louisiana State University Health Sciences Center, LA, USA.

MicroRNAs (miRNAs) regulate the expression of protein-coding genes and represent potential biomarkers for childhood acute lymphoblastic leukemia (ALL). However, information linking miRNAs with their messenger RNA (mRNA) target genes modulating white blood cell (WBC) count is lacking. Here, we analyzed miRNAs and gene expression data from pediatric patients with ALL to identify a signature of miRNAs involved in ALL and their mRNA target genes, molecular networks, and biological pathways modulating WBC. Read More

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http://dx.doi.org/10.1177/1177271917702895DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5397277PMC
April 2017
18 Reads

Hepatocyte Growth Factor/C-Met Axis in Thyroid Cancer: From Diagnostic Biomarker to Therapeutic Target.

Biomark Insights 2017 30;12:1177271917701126. Epub 2017 Mar 30.

Department of Clinical and Experimental Medicine, Unit of Endocrinology, University of Messina, Messina, Italy.

The hepatocyte growth factor (HGF)/c-met axis plays a crucial role in cancer development by promoting cellular proliferation, motility, and morphogenesis, as well as angiogenesis. Different cellular distributions of both the ligand and the receptor in benign vs malignant lesions indicate this biological system as a candidate for a diagnostic biomarker of malignancy occurring in endocrine glands, such as the thyroid and pituitary. Furthermore, the HGF/c-met expression may help to identify a subset of patients eligible for potential targeted therapies with HGF/c-met inhibitors or antagonists in thyroid tumour, as well as in other malignancies. Read More

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http://dx.doi.org/10.1177/1177271917701126DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5391983PMC
March 2017
14 Reads

Real-Time Reverse-Transcription Quantitative Polymerase Chain Reaction Assay Is a Feasible Method for the Relative Quantification of Heregulin Expression in Non-Small Cell Lung Cancer Tissue.

Biomark Insights 2017 30;12:1177271917699850. Epub 2017 Mar 30.

Translational Medicine and Clinical Pharmacology, Daiichi Sankyo Pharma Development, Edison, NJ, USA.

In preclinical studies, heregulin () expression was shown to be the most relevant predictive biomarker for response to patritumab, a fully human anti-epidermal growth factor receptor 3 monoclonal antibody. In support of a phase 2 study of erlotinib ± patritumab in non-small cell lung cancer (NSCLC), a reverse-transcription quantitative polymerase chain reaction (RT-qPCR) assay for relative quantification of expression from formalin-fixed paraffin-embedded (FFPE) NSCLC tissue samples was developed and validated and described herein. Test specimens included matched FFPE normal lung and NSCLC and frozen NSCLC tissue, and -positive and -negative cell lines. Read More

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http://dx.doi.org/10.1177/1177271917699850DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5391987PMC
March 2017
29 Reads

A Novel Biomarker Panel to Identify Steroid Resistance in Childhood Idiopathic Nephrotic Syndrome.

Biomark Insights 2017 8;12:1177271917695832. Epub 2017 Mar 8.

Division of Nephrology and Hypertension, Cincinnati Children's Hospital Medical Center, Cincinnati, OH, USA.

Idiopathic nephrotic syndrome (NS) is the most common glomerular disorder of childhood. Response to initial treatment with corticosteroids is an indicator of prognosis, as resistant patients often present more progressive disease. In this cross-sectional pilot study, we set out to discover a panel of noninvasive biomarkers that could distinguish steroid-resistant nephrotic syndrome (SRNS) from steroid-sensitive nephrotic syndrome (SSNS). Read More

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http://dx.doi.org/10.1177/1177271917695832DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5391984PMC
March 2017
11 Reads

An Integrative Genomics Approach for Associating Genome-Wide Association Studies Information With Localized and Metastatic Prostate Cancer Phenotypes.

Biomark Insights 2017 16;12:1177271917695810. Epub 2017 Mar 16.

Department of Biology, Mangosuthu University of Technology, Durban, South Africa.

High-throughput genotyping has enabled discovery of genetic variants associated with an increased risk of developing prostate cancer using genome-wide association studies (GWAS). The goal of this study was to associate GWAS information of patients with primary organ-confined and metastatic prostate cancer using gene expression data and to identify molecular networks and biological pathways enriched for genetic susceptibility variants involved in the 2 disease states. The analysis revealed gene signatures for the 2 disease states and a gene signature distinguishing the 2 patient groups. Read More

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http://dx.doi.org/10.1177/1177271917695810DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5391982PMC
March 2017
17 Reads

Leptomeningeal Carcinomatosis in Gastric Cancer: A Therapeutical Challenge.

Biomark Insights 2017 16;12:1177271917695237. Epub 2017 Mar 16.

Division of Hematology and Oncology, First Internal Medicine Department, Dietrich-Bonhoeffer-Klinikum, Neubrandenburg, Germany.

Leptomeningeal carcinomatosis (LC) is a rare and mainly secondary site of metastasis in solid tumors. In gastric cancer (GC), it is associated with a devastating prognosis, lacking an efficient and standardized treatment approach. We report a case of primary manifestation of LC due to metastatic GC with rapid deterioration and refractory course to conventional and intrathecal chemotherapy. Read More

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http://dx.doi.org/10.1177/1177271917695237DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5391981PMC
March 2017
9 Reads

Expression of Reactive Oxygen Species-Related Transcripts in Egyptian Children With Autism.

Biomark Insights 2017 23;12:1177271917691035. Epub 2017 Feb 23.

Microbial Biotechnology Department, Genetic Engineering Division, National Research Centre, Giza, Egypt.

The molecular basis of the pathophysiological role of oxidative stress in autism is understudied. Herein, we used polymerase chain reaction (PCR) array to analyze transcriptional pattern of 84 oxidative stress genes in peripheral blood mononuclear cell pools isolated from 32 autistic patients (16 mild/moderate and 16 severe) and 16 healthy subjects (each sample is a pool from 4 autistic patients or 4 controls). The PCR array data were further validated by quantitative real-time PCR in 80 autistic children (55 mild/moderate and 25 severe) and 60 healthy subjects. Read More

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http://dx.doi.org/10.1177/1177271917691035DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5391985PMC
February 2017
21 Reads

Cytochrome P-450 1B1 Leu432Val Polymorphism Does Not Show Association With Breast Cancer in Northern Iranian Women With a History of Infertility.

Biomark Insights 2017 20;12:1177271916689058. Epub 2017 Feb 20.

Infertility and Reproductive Health Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran.

The Cytochrome P-4501B1 () Leu432Val polymorphism has been previously shown to be associated with some types of cancer and affects -mediated metabolism of various infertility drugs. To establish the frequency of Leu432Val polymorphism among women with a history of infertility drug use, we studied the genotypes of 147 patients with breast cancer with a history of infertility and 150 cancer-free, infertile women (control group) in Northern Iran. A polymerase chain reaction-based restriction fragment length polymorphism assay was used to detect GG (Val/Val), CG (Leu/Val), and CC (Leu/Leu) genotype frequencies, which did not vary significantly between the 2 patient groups ( = . Read More

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http://dx.doi.org/10.1177/1177271916689058DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5391986PMC
February 2017
17 Reads

Soluble P-selectin as a Biomarker for Infection and Survival in Patients With a Systemic Inflammatory Response Syndrome on the Intensive Care Unit.

Biomark Insights 2017 7;12:1177271916684823. Epub 2017 Feb 7.

Department of Intensive Care Medicine, University Medical Center Utrecht, Utrecht, The Netherlands.

Aims: This study investigated the ability of soluble platelet selectin (sP-selectin) to identify infection and predict 30-day mortality in patients with a systemic inflammatory response syndrome (SIRS) on the intensive care unit.

Methods: Soluble platelet selectin levels were measured daily in the first 48 hours in patients presenting with SIRS. The outcome, proven infection, was established using predefined definitions. Read More

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http://dx.doi.org/10.1177/1177271916684823DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5345948PMC
February 2017
16 Reads

A Comparison of Different Methodologies for the Measurement of Extracellular Vesicles and Milk-derived Particles in Raw Milk from Cows.

Biomark Insights 2016 13;11:147-155. Epub 2016 Dec 13.

Department of Comparative Biomedical Sciences, Royal Veterinary College, London, UK.

Cow's milk is economically important to the agricultural industry with the nutritive value of milk being routinely measured. This does not give full insight into normal mammary tissue turnover during the course of lactation, which could be important for both an understanding of milk production and animal welfare. We have previously demonstrated that submicron particles, including extracellular vesicles (EVs), can be measured in unprocessed cow's milk by flow cytometry and that they correlate with stage of lactation. Read More

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http://dx.doi.org/10.4137/BMI.S38438DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5156550PMC
December 2016
12 Reads

Prognostic Value of MammaPrint in Invasive Lobular Breast Cancer.

Biomark Insights 2016 11;11:139-146. Epub 2016 Dec 11.

Agendia NV, Science Park, Amsterdam, the Netherlands.

Background: MammaPrint® is a microarray-based gene expression test cleared by the US Food and Drug Administration to assess recurrence risk in early-stage breast cancer, aimed to guide physicians in making neoadjuvant and adjuvant treatment decisions. The increase in the incidence of invasive lobular carcinomas (ILCs) over the past decades and the modest representation of ILC in the MammaPrint development data set calls for a stratified survival analysis dedicated to this specific subgroup.

Study Aim: The current study aimed to validate the prognostic value of the MammaPrint test for breast cancer patients with early-stage ILCs. Read More

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http://dx.doi.org/10.4137/BMI.S38435DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5153320PMC
December 2016
20 Reads

Asymmetric Dimethylarginine and Its Relation As a Biomarker in Nephrologic Diseases.

Authors:
Mustafa E Sitar

Biomark Insights 2016 7;11:131-137. Epub 2016 Dec 7.

Faculty of Medicine, Department of Clinical Biochemistry, Maltepe University, Maltepe, Istanbul, Republic of Turkey.

It is encouraging to observe that a search for publications on "asymmetric dimethylarginine (ADMA)" in PubMed, as updated on June 2016, yielded >2500 items, 24 years after a splendid paper published by Vallance et al in which the authors proposed that ADMA accumulation could be a cardiovascular risk factor in chronic kidney diseases. ADMA is the endogenous inhibitor of nitric oxide synthase and is related to endothelial dysfunction, which plays an important role in vascular damage elicited by various cardiometabolic risk factors. Although current knowledge suggests that ADMA has critical central roles in renal diseases, there are still unexplained details. Read More

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http://dx.doi.org/10.4137/BMI.S38434DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5144928PMC
December 2016
9 Reads

Procalcitonin Is a Better Biomarker than C-Reactive Protein in Newborns Undergoing Cardiac Surgery: The PROKINECA Study.

Biomark Insights 2016 3;11:123-129. Epub 2016 Nov 3.

Neonatal Intensive Care Unit Service, Hospital de Sant Joan de Déu Maternal, Fetal and Neonatology Center Barcelona (BCNatal), University of Barcelona, Barcelona, Spain.

Objectives: To assess the kinetics of procalcitonin (PCT) and C-reactive protein (CRP) in newborns after cardiothoracic surgery (CS), with and without cardiopulmonary bypass, and to assess whether PCT was better than CRP in identifying sepsis in the first 72 hours after CS.

Patients And Methods: This is a prospective study of newborns admitted to the neonatal intensive care unit after CS.

Interventions: PCT and CRP were sequentially drawn 2 hours before surgery and at 0, 12, 24, 48, and 72 hours after surgery. Read More

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http://dx.doi.org/10.4137/BMI.S40658DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5096765PMC
November 2016
18 Reads