10,770 results match your criteria Biomacromolecules [Journal]


In Vitro Synthesis and Crystallization of β-1,4-Mannan.

Biomacromolecules 2018 Dec 6. Epub 2018 Dec 6.

In vitro polymerization of β-mannans is a challenging reaction due to the steric hindrance confered by the configuration of mannosyl residues and the thermodynamic instability of the β-anomer. Whatever the approach used to date - whether chemical, or enzymatic with glycosynthases and mannosyltransferases - pure β-1,4-mannans have never been synthesized in vitro. This has limited attempts to investigate their role in the production of plant and algal cell walls, in which they are highly abundant. Read More

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December 2018
1 Read

Aromatic Nitrogen Mustard-Based Autofluorescent Amphiphilic Brush Copolymer as pH-Responsive Drug Delivery Vehicle.

Biomacromolecules 2018 Dec 6. Epub 2018 Dec 6.

Delivery of clinically approved nonfluorescent drugs is facing challenges because it is difficult to monitor the intracellular drug delivery without incorporating any integrated fluorescence moiety into the drug carrier. The present investigation reports the synthesis of a pH-responsive autofluorescent polymeric nano-scaffold for the administration of nonfluorescent aromatic nitrogen mustard chlorambucil (CBL) drug into the cancer cells. Copolymerization of poly(ethylene glycol) (PEG) appended styrene and CBL conjugated N-substituted maleimide monomers enables the formation of well-defined luminescent alternating copolymer. Read More

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December 2018
1 Read

Fluorogenic probes for disease-relevant enzymes.

Chem Soc Rev 2018 Dec 6. Epub 2018 Dec 6.

Key Laboratory for Advanced Materials and Joint International Research Laboratory of Precision Chemistry and Molecular Engineering, Feringa Nobel Prize Scientist Joint Research Center, School of Chemistry and Molecular Engineering, East China University of Science and Technology, 130 Meilong Rd., Shanghai 200237, P. R. China.

Traditional biochemical methods for enzyme detection are mainly based on antibody-based immunoassays, which lack the ability to monitor the spatiotemporal distribution and, in particular, the in situ activity of enzymes in live cells and in vivo. In this review, we comprehensively summarize recent progress that has been made in the development of small-molecule as well as material-based fluorogenic probes for sensitive detection of the activities of enzymes that are related to a number of human diseases. The principles utilized to design these probes as well as their applications are reviewed. Read More

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December 2018

A positive feedback regulation of Heme oxygenase 1 by CELF1 in cardiac myoblast cells.

Biochim Biophys Acta Gene Regul Mech 2018 Nov 30. Epub 2018 Nov 30.

National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China. Electronic address:

As an RNA binding protein, CUG-BP Elav-like family (CELF) has been shown to be critical for heart biological functions. However, no reports have revealed the function of CELF1 in hypertrophic cardiomyopathy (HCM). Hinted by RNA immunoprecipitation-sequencing (RIP-seq) data, the influence of the CELF protein on heme oxygenase-1 (HO-1) expression was tested by modulating CELF1 levels. Read More

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November 2018
1 Read

Structure Studies of the CRISPR-Csm Complex Reveal Mechanism of Co-transcriptional Interference.

Cell 2018 Nov 26. Epub 2018 Nov 26.

Key Laboratory of RNA Biology, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, 100101 Beijing, China; University of Chinese Academy of Sciences, 100049 Beijing, China; State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, 100101 Beijing, China. Electronic address:

Csm, a type III-A CRISPR-Cas interference complex, is a CRISPR RNA (crRNA)-guided RNase that also possesses target RNA-dependent DNase and cyclic oligoadenylate (cOA) synthetase activities. However, the structural features allowing target RNA-binding-dependent activation of DNA cleavage and cOA generation remain unknown. Here, we report the structure of Csm in complex with crRNA together with structures of cognate or non-cognate target RNA bound Csm complexes. Read More

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November 2018

Biodistribution of Filamentous Plant Virus Nanoparticles: Pepino Mosaic Virus versus Potato Virus X.

Biomacromolecules 2018 Dec 5. Epub 2018 Dec 5.

Nanoparticles with high aspect ratios have favorable attributes for drug delivery and bio-imaging applications based on their enhanced tissue penetration and tumor homing properties. Here, we investigated a novel filamentous viral nanoparticle (VNP) based on Pepino mosaic virus (PepMV), a relative of the established platform Potato virus X (PVX). We studied the chemical reactivity of PepMV, produced fluorescent versions of PepMV and PVX, and then evaluated their biodistribution in mouse tumor models. Read More

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December 2018
1 Read

ATP-responsive low-molecular-weight polyethylenimine-based supramolecular assembly via host-guest interaction for gene delivery.

Biomacromolecules 2018 Dec 5. Epub 2018 Dec 5.

In this work, we report on an ATP-responsive low-molecular-weight polyethylenimine (LMW-PEI)-based supramolecular assembly. It formed via host-guest interaction between PEI (MW=1.8 kDa)-α-cyclodextrin (α-CD) conjugates and PEI1. Read More

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December 2018

A straightforward route to superhydrophilic poly(2-oxazoline)s via acylation of well-defined polyethylenimine.

Biomacromolecules 2018 Dec 4. Epub 2018 Dec 4.

Herein, we describe a new method for the synthesis of superhydrophilic poly(2-alkyl-2-oxazoline)s (PAOx) from poly(2-ethyl-2-oxazoline) (PEtOx). A well-defined linear polyethylenimine was prepared from PEtOx by controlled acidic hydrolysis of its side-chains followed by re-acylation with different carboxylic acids. Using this protocol, we obtained a series of new hydrophilic PAOx containing side-chain ether groups with potential in the biomaterials science. Read More

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December 2018
2 Reads

Blended Nanostructured Degradable Mesh with Endometrial Mesenchymal Stem Cells Promotes Tissue Integration and Anti-Inflammatory Response in Vivo for Pelvic Floor Application.

Biomacromolecules 2018 Dec 4. Epub 2018 Dec 4.

The current urogynaecological clinical meshes trigger unfavourable foreign body response which leads to graft failure in the long term. To overcome the present challenge we applied a tissue engineering strategy using endometrial SUSD2+ Mesenchymal stem cells (eMSCs) with high regenerative properties. This study delves deeper into foreign body response to SUSD2+ eMSC based degradable PLACL/Gelatin nanofiber meshes using a mice model targeted at understanding immunomodulation and mesh integration in the long term. Read More

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December 2018
2 Reads

Inhibiting Bacterial Adhesion by Mechanically Modulated Microgel Coatings.

Biomacromolecules 2018 Dec 4. Epub 2018 Dec 4.

Bacterial infection is a severe problem especially when associated with biomedical applications. This study effectively demonstrates that poly-N-isopropylmethacrylamide based microgel coatings prevent bacterial adhesion. The coating preparation via a spraying approach proved to be simple, and both cost and time efficient creating a homogeneous dense microgel monolayer. Read More

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December 2018
1 Read

Multivalent Carbohydrate-Functionalized Polymer Nanocrystals.

Biomacromolecules 2018 Dec 4. Epub 2018 Dec 4.

Nanoparticles with a covalently bound shell of carbohydrate and sulfate groups, respectively, and a polyethylene core were generated by Ni(II)-catalyzed aqueous copolymerization of ethylene with comonomers undec-10-en-1-yl sulfate, undec-10-en-1-yl β-D-glucoside or undec-10-en-1-yl α-D-mannoside, respectively. Via remote substituents of the catalyst, the degree of branching and consequently degree of crystallinity of the polyethylene core could be controlled. This in turn impacts particle shapes, from spherical to anisotropic platelets, as observed by cryo-TEM. Read More

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December 2018
3 Reads

Structural basis for ligand recognition of the human thromboxane A receptor.

Nat Chem Biol 2018 Dec 3. Epub 2018 Dec 3.

CAS Key Laboratory of Receptor Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, China.

Stimulated by thromboxane A, an endogenous arachidonic acid metabolite, the thromboxane A receptor (TP) plays a pivotal role in cardiovascular homeostasis, and thus is considered as an important drug target for cardiovascular disease. Here, we report crystal structures of the human TP bound to two nonprostanoid antagonists, ramatroban and daltroban, at 2.5 Å and 3. Read More

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December 2018

Mechanistic understanding and binding analysis of two-dimensional MoS nanosheets with human serum albumin by the biochemical and biophysical approach.

Spectrochim Acta A Mol Biomol Spectrosc 2018 Nov 22;211:18-25. Epub 2018 Nov 22.

Institute of Environmental Toxicology and Environmental Ecology, Yancheng Teachers University, Yancheng City, Jiangsu Province 224007, People's Republic of China; School of Chemical and Environmental Engineering, Yancheng Teachers University, Yancheng City, Jiangsu Province 224002, People's Republic of China. Electronic address:

With the advent of molybdenum disulfide nanosheets (MoS NSs) for biological applications, their complex interactions with human serum albumin (HSA) need to be understood in great detail for the molecular mechanisms of protein structure and activity. It was observed that MoS NSs quench the intrinsic fluorescence of HSA as a consequence of ground-state complex formation by the electron transfer, van der Waals, and hydrophobic forces. The presence of MoS NSs partly altered the conformation of HSA and destroyed the binding domain of HSA with bilirubin. Read More

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November 2018
1 Read

Convenient synhtesis of hybrid polymer materials by aget-atrp polymerization of pickering emulsions stabilized by cellulose nanocrystals grafted with reactive moieties.

Biomacromolecules 2018 Nov 30. Epub 2018 Nov 30.

We report a novel method to prepare capsules, beads or open-cell materials from Pickering emulsions of monomers, stabilized by cellulose nanocrystals (CNCs) grafted with reactive Isobutyrate bromide moieties (CNC-Br). CNC-Br particles with different hydrophylic/hydrophobic balance at their surface were prepared, and subsequently used to stabilize direct (O/W), inverted (W/O), or double emulsions of Styrene or n-BuA. The different emulsions obtained were subsequently polymerized, by initiating an AGET-ATRP polymerization from the brominated particles surrounding the stabilized droplets. Read More

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November 2018
3 Reads

Deposition Kinetics of Colloidal Manganese Dioxide onto Representative Surfaces in Aquatic Environments: The Role of Humic Acid and Biomacromolecules.

Environ Sci Technol 2018 Nov 30. Epub 2018 Nov 30.

The initial deposition kinetics of colloidal MnO2 on three representative surfaces in aquatic systems (i.e., silica, magnetite, and alumina) in NaNO3 solution were investigated in the presence of model constituents, including humic acid (HA), a polysaccharide (alginate), and a protein (bovine serum albumin (BSA), using laboratory quartz crystal microbalance with dissipation monitoring equipment (QCM-D). Read More

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November 2018
1 Read

Architectural roles of Cren7 in folding crenarchaeal chromatin filament.

Mol Microbiol 2018 Nov 30. Epub 2018 Nov 30.

State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, No. 1 West Beichen Road, Chaoyang District, Beijing, 100101, China.

Archaea have evolved various strategies in chromasomal organization. While histone homologues exist in most archaeal phyla, Cren7 is a chromatin protein conserved in the Crenarchaeota. Here we show that Cren7 preferentially binds DNA with AT-rich sequences over that with GC-rich sequences with a binding size of 6 ~ 7 bp. Read More

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November 2018
1 Read

Reciprocal regulation between lunapark and atlastin facilitates ER three-way junction formation.

Protein Cell 2018 Nov 29. Epub 2018 Nov 29.

Department of Genetics and Cell Biology, College of Life Sciences, Nankai University and Tianjin Key Laboratory of Protein Sciences, Tianjin, 300071, China.

Three-way junctions are characteristic structures of the tubular endoplasmic reticulum (ER) network. Junctions are formed through atlastin (ATL)-mediated membrane fusion and stabilized by lunapark (Lnp). However, how Lnp is preferentially enriched at three-way junctions remains elusive. Read More

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November 2018
3 Reads

Supramolecular Transformation of Metallacycle-Linked Star Polymers Driven by Simple Phosphine Ligand-Exchange Reaction.

J Am Chem Soc 2018 Nov 29. Epub 2018 Nov 29.

As a common phenomenon in biological systems, supramolecular transformations of biomacromolecules lead to specific biological functions as outputs, which thus inspire people to construct biomimetic dynamic systems through supramolecular transformation stratetgy. It should be noted that well-modulating the artificial macromolecules to fine-tune their properties is of great significance yet still remains a big challenge in polymer chemistry. In this study, through the combination of coordination-driven self-assembly and post-assembly ring-opening polymerization, a six-armed star polymer linked by well-defined hexagonal metallacycle as core was successfully prepared. Read More

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November 2018
2 Reads

Importance of Thermally-Induced Aggregation on 19F Magnetic Resonance Imaging of Perfluoropolyether-Based Comb-Shaped Poly(2-oxazoline)s.

Biomacromolecules 2018 Nov 29. Epub 2018 Nov 29.

An understanding of thermally-induced aggregation, and consequent 19F magnetic resonance imaging (MRI) performance is essential for improved design of thermoresponsive 19F MRI contrast agents. Herein we describe a series of novel thermoresponsive perfluoropolyether (PFPE)-based comb-shaped poly(2-oxazoline)s (POxs) with different side-chain structures (2-methyl- (MeOx), 2-ethyl- (EtOx) and 2-(n-propyl)-2-oxazoline (nPrOx)). The comb polymers were prepared through reversible addition-fragmentation chain transfer (RAFT) polymerization of the respective oligo(2-oxazoline)acrylates using a perfluoropolyether macro-RAFT agent. Read More

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November 2018
1 Read

Stella safeguards the oocyte methylome by preventing de novo methylation mediated by DNMT1.

Nature 2018 12 28;564(7734):136-140. Epub 2018 Nov 28.

National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China.

Postnatal growth of mammalian oocytes is accompanied by a progressive gain of DNA methylation, which is predominantly mediated by DNMT3A, a de novo DNA methyltransferase. Unlike the genome of sperm and most somatic cells, the oocyte genome is hypomethylated in transcriptionally inert regions. However, how such a unique feature of the oocyte methylome is determined and its contribution to the developmental competence of the early embryo remains largely unknown. Read More

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December 2018
1 Read

Structure and Dynamics of Biobased Polyester Nanocomposites.

Biomacromolecules 2018 Nov 28. Epub 2018 Nov 28.

The structure and the dynamics of two bio-based polyester polyols are investigated in the bulk and close to surfaces in polymer / layered silicate nanocomposites. The morphology of the neat polymers as well as the structure of the nanohybrids are investigated with X-ray diffraction and their thermal properties are studied by differential scanning calorimetry. One of the investigated polyesters is amorphous whereas the second one is a semi-crystalline polymer with an intriguing thermal behavior. Read More

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November 2018
1 Read

Enhanced photoacoustic and photothermal effect of functionalized polypyrrole nanoparticles for near-infrared theranostic treatment of tumor.

Biomacromolecules 2018 Nov 28. Epub 2018 Nov 28.

Functionalized nanomaterials with near-infrared (NIR) responsive capacity are quite promising for theranostic treatment of tumors, but formation of NIR responsive nanomaterials with enhanced theranostic ability and excellent biocompatibility is still very challenging. Herein, PEGylated indocyanine green (ICG)-loaded polypyrrole nanoparticles (PPI NPs) were designed and successfully formed through selecting polydopamine as the linkage between each component, demonstrating enhanced NIR responsive theranostic ability against tumor. Combining in vitro cell study with in vivo assay, the formed PPI NPs were proved being fantastic biocompatible while effectively internalizing in HeLa cells and retaining in HeLa tumor demonstrated by in vitro flow cytometry/confocal measurement and in vivo photoacoustic imaging assay. Read More

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November 2018

POLYMER CANCEROSTATICS TARGETED BY RECOMBINANT ANTIBODY FRAGMENTS TO GD2-POSITIVE TUMOR CELLS.

Biomacromolecules 2018 Nov 28. Epub 2018 Nov 28.

A water-soluble polymer cancerostatic actively targeted against cancer cells expressing a disialoganglioside antigen GD2 was designed, synthesized and characterized. A polymer conjugate of an anti-tumor drug doxorubicin with a N-(2-hydroxypropyl)methacrylamide-based copolymer was specifically targeted against GD2 antigen-positive tumor cells using a recombinant single chain fragment (scFv) of an anti-GD2 monoclonal antibody. The targeting protein ligand was attached to the polymer-drug conjugate either via a covalent bond between the amino groups of the protein using a traditional non-specific aminolytic reaction with a reactive polymer precursor or via a non-covalent but highly specific interaction between bungarotoxin covalently linked to the polymer and the recombinant scFv modified with a C-terminal bungarotoxin-binding peptide. Read More

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November 2018
4 Reads

The Impact of Branching on the Solution Behavior and Serum Stability of Star-like Block Copolymers.

Biomacromolecules 2018 Nov 26. Epub 2018 Nov 26.

The size control of nanomedicines for tumor diagnosis and therapy is of high importance, since it enables or disables deep and sufficient tumor penetration. Amphiphilic star shaped block copolypept(o)ides offer substantial promise to precisely adjust the hydrophobic core and the hydrophilic corona, independent of each other, and therefore, simultaneously control the size dimension in the interesting size range from 10 - 30 nm. To gain access to core-shell structures of such sizes, 3-arm and 6-arm PeptoStars, based on poly(γ-t-butyloxycarbonyl-L-glutamate)-b-polysarcosine (pGlu(OtBu)-b-pSar), were prepared via controlled living ring-opening polymerization (ROP) of the corresponding N-carboxyanhydrides. Read More

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November 2018
3 Reads

Gold Nanoparticle-Stabilized, Tyrosine-Rich Peptide Self-Assemblies and Their Catalytic Activities in the Reduction of 4-Nitrophenol.

Biomacromolecules 2018 Nov 26. Epub 2018 Nov 26.

Department of Chemical Engineering, Division of Chemical and Biological Engineering , Kangwon National University , Gangwon-Do 24341 , Republic of Korea.

Peptides are suitable candidates for templates in the fabrication of various metal nanoparticles (NPs) because of their metal-binding ability and templating effect, which impart physicochemical properties to the produced nanoparticles. Peptide-binding gold nanoparticles (AuNPs) show high catalytic activity that permits their application in oxidation or reduction reactions. Herein, we prepared morphology-controllable AuNPs stabilized by self-assembled tyrosine-rich peptides (YC) by varying the pH and YC peptide/Au concentration ratio in 2-( N-morpholino)ethanesulfonic acid (MES) buffer solution. Read More

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November 2018
1 Read

Current Status of Development and Intellectual Properties of Biomimetic Medical Materials.

Adv Exp Med Biol 2018 ;1064:377-399

Department of Chemical & Biomolecular Engineering, Seoul National University of Science and Technology (Seoul Tech), Seoul, South Korea.

Biomimetic medical materials are the biomaterials which mimic the important characteristic features of natural material/tissue structures or architectures and are mainly used in biomedical field for their applications in tissue regeneration, medical devices, biosensors and drug delivery. It is one of the leading research topics which have the ability to replace the existing biomaterials and medical devices and to development new biomaterials. The innovation and development in this research area are growing quickly because of the state-of-the-art techniques like nanobiotechnology, biosensors, tissue engineering and regenerative medicine, and 3D (bio)printing. Read More

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January 2018
3 Reads

Study of interaction between citrate-coated silver nanoparticles and gamma globulin using spectroscopic method.

J Biomol Struct Dyn 2018 Nov 23:1-18. Epub 2018 Nov 23.

a Hubei Key Laboratory for Processing and Application of Catalytic Materials , Huanggang Normal University , Huanggang , 438000 , Hubei , China.

Studies on the interactions between nanomaterials and biomacromolecules have attracted great attention since it is helpful for the development of functional nanomaterials. Here, the citrate-coated silver nanoparticles (AgNPs) with a diameter of 25.4 ± 2. Read More

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November 2018
1 Read

Long noncoding RNA lncAIS downregulation in mesenchymal stem cells is implicated in the pathogenesis of adolescent idiopathic scoliosis.

Cell Death Differ 2018 Nov 21. Epub 2018 Nov 21.

Department of Orthopedics, Peking Union Medical College Hospital, Beijing, P.R. China.

Adolescent idiopathic scoliosis (AIS) is a complex, three dimensional deformity of the spine that commonly occurs in pubescent girls. Abnormal osteogenic differentiation of mesenchymal stem cells (MSCs) is implicated in the pathogenesis of AIS. However, the biological roles of long noncoding RNAs (lncRNAs) in the regulation of osteogenic differentiation of MSCs are unknown. Read More

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November 2018
10 Reads
8.180 Impact Factor

Coiled-coil 1-mediated fastening of the neck and motor domains for kinesin-3 autoinhibition.

Proc Natl Acad Sci U S A 2018 Nov 21. Epub 2018 Nov 21.

National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, 100101 Beijing, China;

In kinesin-3, the coiled-coil 1 (CC1) can sequester the preceding neck coil (NC) for autoinhibition, but the underlying mechanism is poorly understood. Here, we determined the structures of the uninhibited motor domain (MD)-NC dimer and inhibited MD-NC-CC1 monomer of kinesin-3 KIF13B. In the MD-NC-CC1 monomer, CC1 is broken into two short helices that unexpectedly interact with both the NC and the MD. Read More

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November 2018
3 Reads

Chemically Induced Morphogenesis of P22 Virus like Particles by the Surfactant Sodium Dodecyl Sulfate.

Biomacromolecules 2018 Nov 21. Epub 2018 Nov 21.

In the infectious P22 bacteriophage the packaging of DNA into the initially formed procapsid triggers a remarkable morphological transformation where the capsid expands from 58 nm to 64 nm. Along with the increase in size, this maturation also provides greater stability to the capsid and initiates the release of scaffolding protein (SP).2, 4 In the P22 virus-like particle (VLP) this transformation can be mimicked in vitro by heating the procapsid particles to 65ºC or by treatment with sodium dodecyl sulfate (SDS). Read More

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November 2018
1 Read

Lubrication and Wear Protection of Micro-Structured Hydrogels using Bioinspired Fluids.

Biomacromolecules 2018 Nov 21. Epub 2018 Nov 21.

We report the fabrication and the use of a bioinspired synovial fluid acting as a lubricant fluid and anti-wear agent at soft and porous chitosan hydrogel tribopairs. This synthetic synovial fluid is composed of sodium hyaluronate (HA) and a bottle-brush polymer (BB) having a polycationic attachment group and polyzwitterionic pendant chains. 2. Read More

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November 2018
2 Reads

Self-assembly of stimuli-responsive biohybrid synthetic-b-recombinant block copolypeptides.

Biomacromolecules 2018 Nov 20. Epub 2018 Nov 20.

The synthesis and original thermoresponsive behavior of hybrid diblock copolypeptides composed of synthetic and recombinant polypeptides are herein reported. A thermoresponsive recombinant elastin-like polypeptide was used as macroinitiator to synthesize a range of poly(L-glutamic acid)-block-elastin-like polypeptide (PGlu-b-ELP) diblock copolypeptides with variable PGlu block lengths. Their temperature-triggered self-assembly in water and in phosphate-buffered saline (PBS) was investigated at the macroscopic scale using complementary techniques such as turbidimetry, dynamic and static light scattering, small-angle neutron scattering, and at the molecular scale by 1H NMR and circular dichroism (CD). Read More

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November 2018
1 Read

Nanoscale patterning of surfaces via DNA directed spider silk assembly.

Biomacromolecules 2018 Nov 20. Epub 2018 Nov 20.

Oligonucleotide-spider silk conjugates can be placed on silicon wafers by complementary DNA strands, which are coupled chemically to the surface. Such specific immobilization of spider silk proteins allows, the nucleation and guided growth of β-sheet-rich nanofibrils in the presence of phosphate ions on the surface. Adjustment of the concentration of the immobilized conjugate, phosphate concentration and time of the assembly reaction enables control over fibril surface density and length. Read More

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November 2018
1 Read

Solvent-Controlled Spatial Distribution of SI AGET ATRP Grafted Polymers in Lignocellulosic Materials.

Biomacromolecules 2018 Nov 20. Epub 2018 Nov 20.

In the current quest for the design of advanced complex materials, the functionalization of biological materials having hierarchical structures has been of high interest. In the case of lignocellulosic materials, various modification techniques have allowed to obtain materials with outstanding properties. However, the control over the spatial distribution of the modification inside the wood scaffold, which is an important parameter to obtain the desired properties, has yet to be understood. Read More

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November 2018
1 Read

Enzymatic Activity in Fractal Networks of Self-assembling Peptides.

Biomacromolecules 2018 Nov 20. Epub 2018 Nov 20.

The tissue environment is exceptionally complex, with well-controlled biochemical communication occurring between similar and dissimilar cells, as well as, between these cells and local extracellular matrices (ECM). In order to build an artificial ECM that can directly affect regional cell populations, a designer system should allow for controlled degradation, molecular release, and reorganization as related to local cellular function. (RADA)4 self-assembling peptide (SAP) hydrogels are excellent candidates for precisely tuned ECMs, or nanoscaffolds, with several beneficial qualities. Read More

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November 2018
3 Reads

Visualizing Intracellular Organelle and Cytoskeletal Interactions at Nanoscale Resolution on Millisecond Timescales.

Cell 2018 Nov 25;175(5):1430-1442.e17. Epub 2018 Oct 25.

National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China; College of Life Sciences, University of Chinese Academy of Sciences, Beijing 100049, China; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA. Electronic address:

In eukaryotic cells, organelles and the cytoskeleton undergo highly dynamic yet organized interactions capable of orchestrating complex cellular functions. Visualizing these interactions requires noninvasive, long-duration imaging of the intracellular environment at high spatiotemporal resolution and low background. To achieve these normally opposing goals, we developed grazing incidence structured illumination microscopy (GI-SIM) that is capable of imaging dynamic events near the basal cell cortex at 97-nm resolution and 266 frames/s over thousands of time points. Read More

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November 2018
6 Reads

Structural insights into substrate selectivity, catalytic mechanism and redox regulation of rice photosystem II core phosphatase.

Mol Plant 2018 Nov 16. Epub 2018 Nov 16.

National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, P. R. China; University of Chinese Academy of Sciences, Beijing 100049, P. R. China. Electronic address:

Photosystem II (PSII) core phosphatase (PBCP) selectively dephosphorylates PSII core proteins including D1, D2, CP43 and PsbH. The function of PBCP is required for efficient degradation of D1 protein in the repair cycle of PSII, a supramolecular machinery highly susceptible to photodamage during oxygenic photosynthesis. Here we present structural and functional studies of PBCP from Oryza sativa (OsPBCP). Read More

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November 2018
6 Reads

Instructed Assembly of Peptides for Intracellular Enzyme Sequestration.

J Am Chem Soc 2018 Nov 21. Epub 2018 Nov 21.

Department of Chemistry , Brandeis University , 415 South Street , Waltham , Massachusetts 02454 , United States.

Liquid-like droplets of biomacromolecules are emerging as a fundamental mechanism of cellular signaling, but designing synthetic mimics to form such membraneless organelles remains unexplored. Here we report the use of supramolecular assemblies of small peptides, as a mimic of biomacromolecular condensates, for intracellular sequestration of enzymes on endoplasmic reticulum (ER). Specifically, integrating a short peptide with naproxen (a nonsteroidal anti-inflammatory drug (NSAID) and a ligand of cyclooxygenase-2 (COX-2)) generates an enzymatic substrate that acts as a precursor for instructed assembly. Read More

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November 2018
1 Read

Nature-Inspired and "Water-Skating" Paper and Polyester Substrates Enabled by the Molecular Structure of Poly(γ-stearyl-α,l-glutamate) Homopolypeptide.

Biomacromolecules 2018 Nov 16. Epub 2018 Nov 16.

School of Chemical and Biomolecular Engineering , Georgia Institute of Technology , Atlanta , Georgia 30332 , United States.

We demonstrate that the molecular structure of a synthetic homopolypeptide that resembles the leg architecture of water strider insects is effective to impart flexible polymeric surfaces with superhydrophobic behavior. Filter paper (FP) and polyester (PET) were modified with a coating consisting of low-molecular weight α-helical poly(γ-stearyl-α,l-glutamate) (PSLG, M = 4500 Da) homopolypeptide. PSLG-coated substrates displayed near to and superhydrophobic behavior (≥150°) as reflected by the contact angle values. Read More

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November 2018
4 Reads

Modification of Poly(ethylene 2,5-furandicarboxylate) with Bio-based 1,5-Pentanediol: Significantly Toughened Copolyesters Retaining High Tensile Strength and O2 Barrier Property.

Biomacromolecules 2018 Nov 15. Epub 2018 Nov 15.

Poly(ethylene 2,5-furandicarboxylate) (PEF) is a bio-based polyester characterized by high gas barrier properties as well as high tensile modulus and strength, but poor toughness. Toughening PEF without sacrificing its modulus, strength and gas barrier performance is a great challenge for PEF modification. In this study, high molecular weight random poly(ethylene-co-1,5-pentylene 2,5-furandicarboxylate)s (PEPeFs) were synthesized via melt copolycondensation of 2,5-furandicarboxylic acid (FDCA), ethylene glycol (EG) and 1,5-pentanediol (PeDO), a cheap, bio-based and commercially available odd-carbon comonomer. Read More

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November 2018
1 Read

Use of Light-Degradable Aliphatic Polycarbonate Nanoparticles As Drug Carrier for Photosensitizer.

Biomacromolecules 2018 Nov 29. Epub 2018 Nov 29.

Department of Chemistry , Paderborn University , Warburger Str. 100 , D-33098 Paderborn , Germany.

Aliphatic poly(carbonate)s (APCs) with rapid and controlled degradation upon specific stimulation have great advantages for a variety of biomedical and pharmaceutical applications. In the present work, we reported a new poly(trimethylene carbonate) (PTMC)-based copolymer containing multiple 4,5-dimethoxy-2-nitrobenzyl photo cleavable groups as pendent chains. The six-membered light-responsive cyclic carbonate monomer (LrM) was first prepared from 2-(hydroxymethyl)-2-methylpropane-1,3-diol and 4,5-dimethoxy-2-nitrobenzyl alcohol and then copolymerized with trimethylene carbonate (TMC) by 1,8-diazabicyclo(5. Read More

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November 2018
2 Reads

Blocking-Free and Substrate-Independent Serological Microarray Immunoassays.

Biomacromolecules 2018 Nov 28. Epub 2018 Nov 28.

Laboratory for Chemistry and Physics of Interfaces, Department of Microsystems Engineering (IMTEK) , University of Freiburg , Georges-Köhler-Allee 103 , 79110 Freiburg , Germany.

In bioanalytical applications, many coating strategies have been established for so-called "blocking" of the surfaces. However, most of the procedures developed so far require additional processing steps for surface blocking and small variations in the blocking efficiency result in increased background noise, which lowers the overall sensitivity of an assay. In this study, we demonstrate the preparation of a bioanalytical surface with a thin film of a photo-cross-linkable copolymer that is transformed photochemically into a surface-attached hydrogel network. Read More

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November 2018
4 Reads

Uniform Symmetric and Asymmetric Polymer Nanostructures via Directed Chain Organization.

Biomacromolecules 2018 Nov 28. Epub 2018 Nov 28.

Australian Institute for Bioengineering and Nanotechnology , The University of Queensland , Brisbane QLD 4072 , Australia.

Polymer nanostructures can be designed with specific properties and functions, such as controlled shape, size, chemical composition, and adaptive ability to change shape or size in response to environmental cues. Precise control to organize polymer chains into uniform nonspherical symmetric and asymmetric nanostructures and at scale remains a synthetic challenge. Here, by using the temperature-directed morphology transformation (TDMT) method we show through a systematic organization of polymer chains the synthesis of well-defined asymmetric (i. Read More

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November 2018
1 Read

Avidin-Biotin Cross-Linked Microgel Multilayers as Carriers for Antimicrobial Peptides.

Biomacromolecules 2018 Nov 28. Epub 2018 Nov 28.

Department of Pharmacy , University of Copenhagen , DK-2100 Copenhagen , Denmark.

Herein, we report on the formation of cross-linked antimicrobial peptide-loaded microgel multilayers. Poly(ethyl acrylate- co-methacrylic acid) microgels were synthesized and functionalized with biotin to enable the formation of microgel multilayers cross-linked with avidin. Microgel functionalization and avidin cross-linking were verified with infrared spectroscopy, dynamic light scattering, and z-potential measurements, while multilayer formation (up to four layers) was studied with null ellipsometry and quartz crystal microbalance with dissipation (QCM-D). Read More

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November 2018
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Ultra-stable super-resolution fluorescence cryo-microscopy for correlative light and electron cryo-microscopy.

Sci China Life Sci 2018 Nov 2. Epub 2018 Nov 2.

College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, China.

Remarkable progress in correlative light and electron cryo-microscopy (cryo-CLEM) has been made in the past decade. A crucial component for cryo-CLEM is a dedicated cryo-fluorescence microscope (cryo-FM). Here, we describe an ultra-stable superresolution cryo-FM that exhibits excellent thermal and mechanical stability. Read More

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November 2018
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Structural insights into Cas13b-guided CRISPR RNA maturation and recognition.

Cell Res 2018 Dec 13;28(12):1198-1201. Epub 2018 Nov 13.

The Key Laboratory of Innate Immune Biology of Fujian Province, Biomedical Research Center of South China, College of Life Sciences, Fujian Normal University, Fuzhou, 350117, China.

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December 2018
13 Reads

A facile approach for the reduction of 4‑nitrophenol and degradation of congo red using gold nanoparticles or laccase decorated hybrid inorganic nanoparticles/polymer-biomacromolecules vesicles.

Mater Sci Eng C Mater Biol Appl 2019 Jan 1;94:524-533. Epub 2018 Oct 1.

MIIT Key Laboratory of Critical Materials Technology for New Energy Conversion and Storage, State Key Laboratory of Urban Water Resource and Environment, School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin, Heilongjiang 150001, China. Electronic address:

Catalytic reduction of toxic 4‑nitrophenol to 4‑aminophenol and dye wastewater treatment over rapid, convenient gold nanoparticles or laccase decorated hybrid vesicles catalysts has attracted much attention. In current work, a stable building block was designed with inorganic gold nanoparticles and nano-conjugates; and a hybrid giant vesicles (AuNPs@vesicles) was self-assembled by using Pickering emulsion method. The vesicles were characterized by SEM, TEM, UV-vis and DLS measurements. Read More

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January 2019
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Topological Transition in Spontaneously Formed Cellulosic Liquid-Crystalline Microspheres in a w/o Emulsion.

Biomacromolecules 2018 Nov 13. Epub 2018 Nov 13.

A cholesteric liquid crystal (ChLC) in a confined space has attracted significant scientific interest and has potential applications. ChLC microspheres have been obtained by devising processing such as a microfluidic technique. Herein, we adopted the self-assembly approach for the spontaneous formation of cellulosic ChLC microspheres. Read More

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November 2018
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Structural basis of AimP signaling molecule recognition by AimR in Spbeta group of bacteriophages.

Protein Cell 2018 Nov 12. Epub 2018 Nov 12.

The Key Laboratory of Innate Immune Biology of Fujian Province, Biomedical Research Center of South China, College of Life Sciences, Fujian Normal University, Fuzhou, 350117, China.

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November 2018
11 Reads

Cinnamaldehyde-Based Poly(ester-thioacetal) To Generate Reactive Oxygen Species for Fabricating Reactive Oxygen Species-Responsive Nanoparticles.

Biomacromolecules 2018 Nov 16. Epub 2018 Nov 16.

National Engineering Research Center for Biomaterials , Sichuan University , Chengdu 610064 , China.

Due to the high oxidative stress of the tumor microenvironment, more and more researchers have been devoted to reactive oxygen species (ROS)-responsive nanodrug delivery systems for anticancer therapy. Herein, a ROS-responsive moiety, thioacetal, was synthesized, and cinnamaldehyde (CA) was introduced in the polymer chain to trigger the generation of ROS to expect the enhancement of the ROS-responsive effect. The poly(ester-thioacetal) mPEG2k - b-(NTA-HD) polymer, its self-assembled micelles, and the ROS-responsive behavior were characterized by H NMR and DLS. Read More

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November 2018
3 Reads