11,071 results match your criteria Biology[Journal]


TLR1/2 orchestrate human plasmacytoid predendritic cell response to gram+ bacteria.

PLoS Biol 2019 Apr 24;17(4):e3000209. Epub 2019 Apr 24.

Institut Curie, Centre de Recherche, PSL Research University, Paris, France.

Gram+ infections are worldwide life-threatening diseases in which the pathological role of type I interferon (IFN) has been highlighted. Plasmacytoid predendritic cells (pDCs) produce high amounts of type I IFN following viral sensing. Despite studies suggesting that pDCs respond to bacteria, the mechanisms underlying bacterial sensing in pDCs are unknown. Read More

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http://dx.doi.org/10.1371/journal.pbio.3000209DOI Listing

Nonlinear phenotypic variation uncovers the emergence of heterosis in Arabidopsis thaliana.

PLoS Biol 2019 Apr 24;17(4):e3000214. Epub 2019 Apr 24.

Max Planck Institute for Developmental Biology, Tübingen, Germany.

Heterosis describes the phenotypic superiority of hybrids over their parents in traits related to agronomic performance and fitness. Understanding and predicting nonadditive inheritance such as heterosis is crucial for evolutionary biology as well as for plant and animal breeding. However, the physiological bases of heterosis remain debated. Read More

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http://dx.plos.org/10.1371/journal.pbio.3000214
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http://dx.doi.org/10.1371/journal.pbio.3000214DOI Listing
April 2019
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An elucidation of over a century old enigma in genetics-Heterosis.

PLoS Biol 2019 Apr 24;17(4):e3000215. Epub 2019 Apr 24.

Museum of Comparative Zoology, Harvard University, Cambridge, Massachusetts, United States of America.

Recognition and exploitation of hybrid vigor or heterosis among individual crosses of plants and animals has a long and distinguished history. Its manifestation is influenced by a combination of genetic, epigenetic, phenotypic, and environmental factors. Although heterosis is known to be governed by both dominant and epistatic gene action, its expression is greatly influenced by nonlinear interaction among epigenetic and phenotypic (phenomic) components. Read More

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http://dx.doi.org/10.1371/journal.pbio.3000215DOI Listing

Glutamatergic neurons of the gigantocellular reticular nucleus shape locomotor pattern and rhythm in the freely behaving mouse.

PLoS Biol 2019 Apr 24;17(4):e2003880. Epub 2019 Apr 24.

Centre de Recherche du CHU de Québec, CHUL-Neurosciences, Québec (QC), Canada.

Because of their intermediate position between supraspinal locomotor centers and spinal circuits, gigantocellular reticular nucleus (GRN) neurons play a key role in motor command. However, the functional contribution of glutamatergic GRN neurons in initiating, maintaining, and stopping locomotion is still unclear. Combining electromyographic recordings with optogenetic manipulations in freely behaving mice, we investigate the functional contribution of glutamatergic brainstem neurons of the GRN to motor and locomotor activity. Read More

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http://dx.doi.org/10.1371/journal.pbio.2003880DOI Listing

The Expression of Adipose Tissue-Derived Cardiotrophin-1 in Humans with Obesity.

Biology (Basel) 2019 Apr 13;8(2). Epub 2019 Apr 13.

Pennington Biomedical Research Center, Baton Rouge, LA 70808, USA.

Cardiotrophin-1 (CT-1) is a gp130 cytokine that was previously characterized for its effects on cardiomyocytes and identified as a marker of heart failure. More recent studies reported elevated circulating levels of CT-1 in humans with obesity and metabolic syndrome (MetS). However, a subsequent rodent study implicated CT-1 as a potential therapeutic target for obesity and MetS. Read More

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http://dx.doi.org/10.3390/biology8020024DOI Listing

Request a woman scientist: A database for diversifying the public face of science.

PLoS Biol 2019 Apr 23;17(4):e3000212. Epub 2019 Apr 23.

500 Women Scientists, Boulder, Colorado, United States of America.

A global online register of women scientists, ready to share their science, was established by a cohort of volunteer women from the grassroots organization 500 Women Scientists on January 17th, 2018. In less than one year, the database "Request a Woman Scientist" comprised over 7,500 women from 174 scientific disciplines and 133 countries. The database is built upon a voluntary questionnaire regarding career stage, degree, scientific discipline, geographic location, and other self-identifying dimensions of representation. Read More

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http://dx.doi.org/10.1371/journal.pbio.3000212DOI Listing

How does a protein's structure spell the difference between health and disease? Our journey to understand glaucoma-associated myocilin.

PLoS Biol 2019 Apr 22;17(4):e3000237. Epub 2019 Apr 22.

School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, Georgia, United States of America.

Over 20 years ago, alterations to the protein myocilin were confirmed to be linked to a heritable form of the prevalent eye disease, glaucoma, and 10 years ago, my lab set out to develop a deeper understanding of myocilin in its normal and diseased state. We have made strides in understanding how genetic mutations in myocilin likely lead to disease, but unlocking myocilin's biological function is still an elusive goalpost. Is normal myocilin unimportant in the human body? Are scientists using the wrong methods to study myocilin biology? Here, I discuss my scientific journey into understanding one small part of the fascinating organ that is the eye. Read More

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http://dx.doi.org/10.1371/journal.pbio.3000237DOI Listing
April 2019
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Molecular understanding of calcium permeation through the open Orai channel.

PLoS Biol 2019 Apr 22;17(4):e3000096. Epub 2019 Apr 22.

State Key Laboratory of Medicinal Chemical Biology and College of Life Sciences, Nankai University, Tianjin, China.

The Orai channel is characterized by voltage independence, low conductance, and high Ca2+ selectivity and plays an important role in Ca2+ influx through the plasma membrane (PM). How the channel is activated and promotes Ca2+ permeation is not well understood. Here, we report the crystal structure and cryo-electron microscopy (cryo-EM) reconstruction of a Drosophila melanogaster Orai (dOrai) mutant (P288L) channel that is constitutively active according to electrophysiology. Read More

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http://dx.plos.org/10.1371/journal.pbio.3000096
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April 2019
3 Reads

NIR absorbing reduced graphene oxide for photothermal radiotherapy for treatment of esophageal cancer.

J Photochem Photobiol B 2019 Mar 22;194:188-193. Epub 2019 Mar 22.

Department of Radiotherapy, Linyi Cancer Hospital, Shandong, China. Electronic address:

Theranostic agents were drawing a huge attention in the personalized medication. In this study, we established a facile technique, plant extract-based technique for the synthesis of reduced nano-graphene oxide (RNGO) with low cytotoxicity. We formed platforms of photothermal (PT) therapy and further explained that the synthesized RNGO can be utilized as ready to use PT therapy without any additional surface adjustment. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S10111344193020
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http://dx.doi.org/10.1016/j.jphotobiol.2019.03.014DOI Listing
March 2019
2 Reads

Synthesis of Nomega-Nitro-l-arginine methyl ester modified reduced graphene oxide nanosheets and their protective action on experimental preeclampsia in mice.

J Photochem Photobiol B 2019 Mar 22;194:183-187. Epub 2019 Mar 22.

Department of Gynaecology and Obstetrics, Jinan Maternal and Child Health Hospital of Shandong Province, Shandong, China. Electronic address:

The purpose of this study is to synthesize Nomega-Nitro-L-arginine methyl ester (L-NAME) adsorbed reduced graphene oxide (RGO) sheets and demonstrate their protective impact on the detrimental effect detected in investigational pre-eclampsia, a prerequisite where increase in oxidative stress and decreased Nitric Oxide (NO) production were present. The synthesized graphene sheets were studies by using various characterization techniques. Later, a prerequisite similar to pre-eclampsia has been induced through chronic inhibition of NO fabrication using a 60 mg/kg/day L-NAME, orally in pregnant mice. Read More

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http://dx.doi.org/10.1016/j.jphotobiol.2019.03.013DOI Listing

How host genetics dictates successful viral zoonosis.

PLoS Biol 2019 Apr 19;17(4):e3000217. Epub 2019 Apr 19.

BioFrontiers Institute and Department of Molecular, Cellular, and Developmental Biology, University of Colorado Boulder, Boulder, Colorado, United States of America.

Viruses of wild and domestic animals can infect humans in a process called zoonosis, and these events can give rise to explosive epidemics such as those caused by the HIV and Ebola viruses. While humans are constantly exposed to animal viruses, those that can successfully infect and transmit between humans are exceedingly rare. The key event in zoonosis is when an animal virus begins to replicate (one virion making many) in the first human subject. Read More

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http://dx.plos.org/10.1371/journal.pbio.3000217
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April 2019
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Regulation of the apical extension morphogenesis tunes the mechanosensory response of microvilliated neurons.

PLoS Biol 2019 Apr 19;17(4):e3000235. Epub 2019 Apr 19.

Institut du Cerveau et de la Moelle épinière (ICM), Inserm U 1127, CNRS UMR 7225, Sorbonne Université, Paris, France.

Multiple types of microvilliated sensory cells exhibit an apical extension thought to be instrumental in the detection of sensory cues. The investigation of the mechanisms underlying morphogenesis of sensory apparatus is critical to understand the biology of sensation. Most of what we currently know comes from the study of the hair bundle of the inner ear sensory cells, but morphogenesis and function of other sensory microvilliated apical extensions remain poorly understood. Read More

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http://dx.doi.org/10.1371/journal.pbio.3000235DOI Listing

Epigenetic regulator G9a provides glucose as a sweet key to stress resistance.

PLoS Biol 2019 Apr 19;17(4):e3000236. Epub 2019 Apr 19.

Centre for Molecular Medicine and Therapeutics, BC Children's Hospital Research Institute, Department of Medical Genetics, The University of British Columbia, Vancouver, British Columbia, Canada.

The ability to adapt to acute and chronic stress is important for organisms to thrive in evolutionary niches and for cells to survive in adverse conditions. The regulatory networks that control stress responses are evolutionarily conserved, and many factors that selectively activate stress responses have been identified. Less well understood are mechanisms that guard against unnecessary induction of cytoprotective factors and that connect stress responses with cellular metabolism to control energy expenditure during stress. Read More

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http://dx.plos.org/10.1371/journal.pbio.3000236
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http://dx.doi.org/10.1371/journal.pbio.3000236DOI Listing
April 2019
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Task-related hemodynamic responses are modulated by reward and task engagement.

PLoS Biol 2019 Apr 19;17(4):e3000080. Epub 2019 Apr 19.

Department of Neuroscience, Columbia University, New York, New York, United States of America.

Hemodynamic recordings from visual cortex contain powerful endogenous task-related responses that may reflect task-related arousal, or "task engagement" distinct from attention. We tested this hypothesis with hemodynamic measurements (intrinsic-signal optical imaging) from monkey primary visual cortex (V1) while the animals' engagement in a periodic fixation task over several hours was varied through reward size and as animals took breaks. With higher rewards, animals appeared more task-engaged; task-related responses were more temporally precise at the task period (approximately 10-20 seconds) and modestly stronger. Read More

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http://dx.plos.org/10.1371/journal.pbio.3000080
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April 2019
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Mechanisms of tethering and cargo transfer during epididymosome-sperm interactions.

BMC Biol 2019 Apr 18;17(1):35. Epub 2019 Apr 18.

Priority Research Centre for Reproductive Science, School of Environmental and Life Sciences, Discipline of Biological Sciences, The University of Newcastle, University Drive, Callaghan, NSW, 2308, Australia.

Background: The mammalian epididymis is responsible for the provision of a highly specialized environment in which spermatozoa acquire functional maturity and are subsequently stored in preparation for ejaculation. Making important contributions to both processes are epididymosomes, small extracellular vesicles released from the epididymal soma via an apocrine secretory pathway. While considerable effort has been focused on defining the cargo transferred between epididymosomes and spermatozoa, comparatively less is known about the mechanistic basis of these interactions. Read More

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http://dx.doi.org/10.1186/s12915-019-0653-5DOI Listing
April 2019
2 Reads
7.984 Impact Factor

Photobiomodulation associated to cellular therapy improve wound healing of experimental full thickness burn wounds in rats.

J Photochem Photobiol B 2019 Apr 10;194:174-182. Epub 2019 Apr 10.

Laboratory of Experimental Pathology, Institute of Tropical Pathology and Public Health, Federal University of Goiás, Goiânia, Brazil. Electronic address:

Adipose derived stromal vascular fraction (SVF) is a method of cell therapy potentially applicable for treatment of full thickness burns. Here we investigated if the association of photobiomodulation (PBM) with SVF therapy could improve wound healing in experimentally induced full thickness burn wounds in rats compared to the topical agent 2% silver sulfadiazine in a dose-dependent manner. Sixty-six male Wistar rats were divided in 4 groups containing 5 animals each which received the following treatments: 2% sulfadiazine (SD), SVF, SVF plus PBM at 30 mW (SVFL30), and SVF plus PBM at 100 mW (SVFL100). Read More

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http://dx.doi.org/10.1016/j.jphotobiol.2019.04.003DOI Listing
April 2019
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Harmonization of resting-state functional MRI data across multiple imaging sites via the separation of site differences into sampling bias and measurement bias.

PLoS Biol 2019 Apr 18;17(4):e3000042. Epub 2019 Apr 18.

Brain Information Communication Research Laboratory Group, Advanced Telecommunications Research Institutes International, Kyoto, Japan.

When collecting large amounts of neuroimaging data associated with psychiatric disorders, images must be acquired from multiple sites because of the limited capacity of a single site. However, site differences represent a barrier when acquiring multisite neuroimaging data. We utilized a traveling-subject dataset in conjunction with a multisite, multidisorder dataset to demonstrate that site differences are composed of biological sampling bias and engineering measurement bias. Read More

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http://dx.plos.org/10.1371/journal.pbio.3000042
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April 2019
2 Reads

In vivo assembly and trafficking of olfactory Ionotropic Receptors.

BMC Biol 2019 Apr 17;17(1):34. Epub 2019 Apr 17.

Center for Integrative Genomics, Génopode Building, Faculty of Biology and Medicine, University of Lausanne, CH-1015, Lausanne, Switzerland.

Background: Ionotropic receptors (IRs) are a large, divergent subfamily of ionotropic glutamate receptors (iGluRs) that are expressed in diverse peripheral sensory neurons and function in olfaction, taste, hygrosensation and thermosensation. Analogous to the cell biological properties of their synaptic iGluR ancestors, IRs are thought to form heteromeric complexes that localise to the ciliated dendrites of sensory neurons. IR complexes are composed of selectively expressed 'tuning' receptors and one of two broadly expressed co-receptors (IR8a or IR25a). Read More

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http://dx.doi.org/10.1186/s12915-019-0651-7DOI Listing

Monoclonal antibodies point to Achilles' heel in picornavirus capsid.

Authors:
Mihnea Bostina

PLoS Biol 2019 Apr 17;17(4):e3000232. Epub 2019 Apr 17.

Department of Microbiology and Immunology and Otago Micro and Nano Imaging, University of Otago, Dunedin, New Zealand.

Picornaviruses are small, icosahedral, nonenveloped, positive-sense, single-stranded RNA viruses that form one of the largest and most important viral families. Numerous Picornaviridae members pose serious health or agricultural threats, causing diseases such as poliomyelitis, hepatitis A, or foot-and-mouth disease. The antigenic characterization of picornavirus capsids plays an important role in understanding the mechanism of viral neutralization and the conformational changes associated with genome release, and it can point to regions which can be targeted by small-molecule compounds to be developed as antiviral inhibitors. Read More

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http://dx.doi.org/10.1371/journal.pbio.3000232DOI Listing
April 2019
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Distinct roles of two myosins in C. elegans spermatid differentiation.

PLoS Biol 2019 Apr 16;17(4):e3000211. Epub 2019 Apr 16.

National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China.

During spermatogenesis, interconnected haploid spermatids segregate undesired cellular contents into residual bodies (RBs) before detaching from RBs. It is unclear how this differentiation process is controlled to produce individual spermatids or motile spermatozoa. Here, we developed a live imaging system to visualize and investigate this process in C. Read More

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http://dx.plos.org/10.1371/journal.pbio.3000211
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http://dx.doi.org/10.1371/journal.pbio.3000211DOI Listing
April 2019
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Oxytocin blocks enhanced motivation for alcohol in alcohol dependence and blocks alcohol effects on GABAergic transmission in the central amygdala.

PLoS Biol 2019 Apr 16;17(4):e2006421. Epub 2019 Apr 16.

Neurobiology of Addiction Section, Intramural Research Program, National Institute on Drug Abuse, National Institutes of Health, Baltimore, Maryland, United States of America.

Oxytocin administration has been reported to decrease consumption, withdrawal, and drug-seeking associated with several drugs of abuse and thus represents a promising pharmacological approach to treat drug addiction. We used an established rat model of alcohol dependence to investigate oxytocin's effects on dependence-induced alcohol drinking, enhanced motivation for alcohol, and altered GABAergic transmission in the central nucleus of the amygdala (CeA). Intraperitoneal oxytocin administration blocked escalated alcohol drinking and the enhanced motivation for alcohol in alcohol-dependent but not nondependent rats. Read More

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http://dx.doi.org/10.1371/journal.pbio.2006421DOI Listing
April 2019
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Atractyligenin, a terpenoid isolated from coffee silverskin, inhibits cutaneous photoaging.

J Photochem Photobiol B 2019 Apr 4;194:166-173. Epub 2019 Apr 4.

Department of Fine Chemistry, Cosmetic Industry Coupled Collaboration Center, Cosmetic R&D Center, Seoul National University of Science and Technology, 232 Gongneung-ro, Nowon-gu, Seoul 01811, Republic of Korea. Electronic address:

Ultraviolet (UV) light exposure-induced photoaging of the skin is a multifactorial process involving both extrinsic and intrinsic cellular mechanisms. Several naturally occurring products are known to confer protection against UV light-induced skin damage. Our preliminary studies confirmed that the ethyl acetate fraction of coffee silverskin exhibits inhibitory effects on matrix metalloproteases (MMPs). Read More

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https://linkinghub.elsevier.com/retrieve/pii/S10111344193004
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http://dx.doi.org/10.1016/j.jphotobiol.2019.04.002DOI Listing
April 2019
4 Reads

Deciphering White Adipose Tissue Heterogeneity.

Biology (Basel) 2019 Apr 11;8(2). Epub 2019 Apr 11.

Department of Biomedical Sciences, Heritage College of Osteopathic Medicine, Ohio University, Athens, OH 45701, USA.

Adipose tissue not only stores energy, but also controls metabolism through secretion of hormones, cytokines, proteins, and microRNAs that affect the function of cells and tissues throughout the body. Adipose tissue is organized into discrete depots throughout the body, and these depots are differentially associated with insulin resistance and increased risk of metabolic disease. In addition to energy-dissipating brown and beige adipocytes, recent lineage tracing studies have demonstrated that individual adipose depots are composed of white adipocytes that are derived from distinct precursor populations, giving rise to distinct subpopulations of energy-storing white adipocytes. Read More

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http://dx.doi.org/10.3390/biology8020023DOI Listing
April 2019
3 Reads

Fine-tuned adaptation of embryo-endometrium pairs at implantation revealed by transcriptome analyses in Bos taurus.

PLoS Biol 2019 Apr 12;17(4):e3000046. Epub 2019 Apr 12.

UMR BDR, INRA, ENVA, Université Paris Saclay, Jouy-en-Josas, France.

Interactions between embryo and endometrium at implantation are critical for the progression of pregnancy. These reciprocal actions involve exchange of paracrine signals that govern implantation and placentation. However, it remains unknown how these interactions between the conceptus and the endometrium are coordinated at the level of an individual pregnancy. Read More

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http://dx.plos.org/10.1371/journal.pbio.3000046
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http://dx.doi.org/10.1371/journal.pbio.3000046DOI Listing
April 2019
3 Reads

The architecture of cell differentiation in choanoflagellates and sponge choanocytes.

PLoS Biol 2019 Apr 12;17(4):e3000226. Epub 2019 Apr 12.

Marine Biological Association of the United Kingdom, The Laboratory, Citadel Hill, Plymouth, United Kingdom.

Although collar cells are conserved across animals and their closest relatives, the choanoflagellates, little is known about their ancestry, their subcellular architecture, or how they differentiate. The choanoflagellate Salpingoeca rosetta expresses genes necessary for animal development and can alternate between unicellular and multicellular states, making it a powerful model for investigating the origin of animal multicellularity and mechanisms underlying cell differentiation. To compare the subcellular architecture of solitary collar cells in S. Read More

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http://dx.doi.org/10.1371/journal.pbio.3000226DOI Listing

Feedforward regulation of Myc coordinates lineage-specific with housekeeping gene expression during B cell progenitor cell differentiation.

PLoS Biol 2019 Apr 12;17(4):e2006506. Epub 2019 Apr 12.

Unit of Computational Medicine, Department of Medicine, Solna, Center for Molecular Medicine, Karolinska Institutet, Stockholm, Sweden.

The differentiation of self-renewing progenitor cells requires not only the regulation of lineage- and developmental stage-specific genes but also the coordinated adaptation of housekeeping functions from a metabolically active, proliferative state toward quiescence. How metabolic and cell-cycle states are coordinated with the regulation of cell type-specific genes is an important question, because dissociation between differentiation, cell cycle, and metabolic states is a hallmark of cancer. Here, we use a model system to systematically identify key transcriptional regulators of Ikaros-dependent B cell-progenitor differentiation. Read More

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http://dx.doi.org/10.1371/journal.pbio.2006506DOI Listing
April 2019
4 Reads

Derivation of adult canine intestinal organoids for translational research in gastroenterology.

BMC Biol 2019 Apr 11;17(1):33. Epub 2019 Apr 11.

Departments of Veterinary Clinical Sciences, Iowa State University, Ames, IA, USA.

Background: Large animal models, such as the dog, are increasingly being used for studying diseases including gastrointestinal (GI) disorders. Dogs share similar environmental, genomic, anatomical, and intestinal physiologic features with humans. To bridge the gap between commonly used animal models, such as rodents, and humans, and expand the translational potential of the dog model, we developed a three-dimensional (3D) canine GI organoid (enteroid and colonoid) system. Read More

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http://dx.doi.org/10.1186/s12915-019-0652-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6460554PMC
April 2019
4 Reads

Dhh1 promotes autophagy-related protein translation during nitrogen starvation.

PLoS Biol 2019 Apr 11;17(4):e3000219. Epub 2019 Apr 11.

Life Sciences Institute, and the Department of Molecular, Cellular and Developmental Biology, University of Michigan, Ann Arbor, Michigan, United States of America.

Macroautophagy (hereafter autophagy) is a well-conserved cellular process through which cytoplasmic components are delivered to the vacuole/lysosome for degradation and recycling. Studies have revealed the molecular mechanism of transcriptional regulation of autophagy-related (ATG) genes upon nutrient deprivation. However, little is known about their translational regulation. Read More

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http://dx.plos.org/10.1371/journal.pbio.3000219
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http://dx.doi.org/10.1371/journal.pbio.3000219DOI Listing
April 2019
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Publication reform to safeguard wildlife from researcher harm.

PLoS Biol 2019 Apr 11;17(4):e3000193. Epub 2019 Apr 11.

Department of Geography, University of Victoria, Victoria, British Columbia, Canada.

Despite abundant focus on responsible care of laboratory animals, we argue that inattention to the maltreatment of wildlife constitutes an ethical blind spot in contemporary animal research. We begin by reviewing significant shortcomings in legal and institutional oversight, arguing for the relatively rapid and transformational potential of editorial oversight at journals in preventing harm to vertebrates studied in the field and outside the direct supervision of institutions. Straightforward changes to animal care policies in journals, which our analysis of 206 journals suggests are either absent (34%), weak, incoherent, or neglected by researchers, could provide a practical, effective, and rapidly imposed safeguard against unnecessary suffering. Read More

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http://dx.doi.org/10.1371/journal.pbio.3000193DOI Listing
April 2019
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Mouse screen reveals multiple new genes underlying mouse and human hearing loss.

PLoS Biol 2019 Apr 11;17(4):e3000194. Epub 2019 Apr 11.

Wellcome Trust Sanger Institute, Hinxton, United Kingdom.

Adult-onset hearing loss is very common, but we know little about the underlying molecular pathogenesis impeding the development of therapies. We took a genetic approach to identify new molecules involved in hearing loss by screening a large cohort of newly generated mouse mutants using a sensitive electrophysiological test, the auditory brainstem response (ABR). We review here the findings from this screen. Read More

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http://dx.doi.org/10.1371/journal.pbio.3000194DOI Listing
April 2019
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Enabling cell-type-specific behavioral epigenetics in Drosophila: a modified high-yield INTACT method reveals the impact of social environment on the epigenetic landscape in dopaminergic neurons.

BMC Biol 2019 Apr 10;17(1):30. Epub 2019 Apr 10.

Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.

Background: Epigenetic mechanisms play fundamental roles in brain function and behavior and stressors such as social isolation can alter animal behavior via epigenetic mechanisms. However, due to cellular heterogeneity, identifying cell-type-specific epigenetic changes in the brain is challenging. Here, we report the first use of a modified isolation of nuclei tagged in specific cell type (INTACT) method in behavioral epigenetics of Drosophila melanogaster, a method we call mini-INTACT. Read More

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https://bmcbiol.biomedcentral.com/articles/10.1186/s12915-01
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http://dx.doi.org/10.1186/s12915-019-0646-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6456965PMC
April 2019
1 Read

Correction to: Q&A: insulin secretion and type 2 diabetes: why do β-cells fail?

BMC Biol 2019 Apr 9;17(1):32. Epub 2019 Apr 9.

Department of Physiology, Anatomy & Genetics, University of Oxford, Parks Road, Oxford, OX1 3PT, UK.

Upon publication of the original article [1], the authors noticed that they had accidently omitted to acknowledge funding from the European Research Council. Read More

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http://dx.doi.org/10.1186/s12915-019-0650-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6456947PMC

The architecture of intra-organism mutation rate variation in plants.

PLoS Biol 2019 Apr 9;17(4):e3000191. Epub 2019 Apr 9.

State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, China.

Given the disposability of somatic tissue, selection can favor a higher mutation rate in the early segregating soma than in germline, as seen in some animals. Although in plants intra-organismic mutation rate heterogeneity is poorly resolved, the same selectionist logic can predict a lower rate in shoot than in root and in longer-lived terminal tissues (e.g. Read More

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http://dx.doi.org/10.1371/journal.pbio.3000191DOI Listing
April 2019
3 Reads

Infection-generated electric field in gut epithelium drives bidirectional migration of macrophages.

PLoS Biol 2019 Apr 9;17(4):e3000044. Epub 2019 Apr 9.

Department of Dermatology, School of Medicine, University of California, Davis, Sacramento, California, United States of America.

Many bacterial pathogens hijack macrophages to egress from the port of entry to the lymphatic drainage and/or bloodstream, causing dissemination of life-threatening infections. However, the underlying mechanisms are not well understood. Here, we report that Salmonella infection generates directional electric fields (EFs) in the follicle-associated epithelium of mouse cecum. Read More

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http://dx.doi.org/10.1371/journal.pbio.3000044DOI Listing
April 2019
2 Reads

Tubulin mRNA stability is sensitive to change in microtubule dynamics caused by multiple physiological and toxic cues.

PLoS Biol 2019 Apr 9;17(4):e3000225. Epub 2019 Apr 9.

Department of Systems Biology, Harvard Medical School, Boston, Massachusetts, United States of America.

The localization, mass, and dynamics of microtubules are important in many processes. Cells may actively monitor the state of their microtubules and respond to perturbation, but how this occurs outside mitosis is poorly understood. We used gene-expression analysis in quiescent cells to analyze responses to subtle and strong perturbation of microtubules. Read More

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http://dx.doi.org/10.1371/journal.pbio.3000225DOI Listing

Exact replication: Foundation of science or game of chance?

PLoS Biol 2019 Apr 9;17(4):e3000188. Epub 2019 Apr 9.

Center for Stroke Research, Charité-Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin, Germany.

The need for replication of initial results has been rediscovered only recently in many fields of research. In preclinical biomedical research, it is common practice to conduct exact replications with the same sample sizes as those used in the initial experiments. Such replication attempts, however, have lower probability of replication than is generally appreciated. Read More

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http://dx.plos.org/10.1371/journal.pbio.3000188
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http://dx.doi.org/10.1371/journal.pbio.3000188DOI Listing
April 2019
1 Read

Meta-transcriptomics reveals a diverse antibiotic resistance gene pool in avian microbiomes.

BMC Biol 2019 Apr 8;17(1):31. Epub 2019 Apr 8.

Marie Bashir Institute for Infectious Diseases and Biosecurity and Sydney Medical School, The University of Sydney, Sydney, NSW, 2006, Australia.

Background: Antibiotic resistance is rendering common bacterial infections untreatable. Wildlife can incorporate and disperse antibiotic-resistant bacteria in the environment, such as water systems, which in turn serve as reservoirs of resistance genes for human pathogens. Anthropogenic activity may contribute to the spread of bacterial resistance cycling through natural environments, including through the release of human waste, as sewage treatment only partially removes antibiotic-resistant bacteria. Read More

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https://bmcbiol.biomedcentral.com/articles/10.1186/s12915-01
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http://dx.doi.org/10.1186/s12915-019-0649-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6454771PMC
April 2019
4 Reads

The amplitude in periodic neural state trajectories underlies the tempo of rhythmic tapping.

PLoS Biol 2019 Apr 8;17(4):e3000054. Epub 2019 Apr 8.

Instituto de Neurobiología, Universidad Nacional Autónoma de México, Campus Juriquilla, Querétaro, México.

Our motor commands can be exquisitely timed according to the demands of the environment, and the ability to generate rhythms of different tempos is a hallmark of musical cognition. Yet, the neuronal underpinnings behind rhythmic tapping remain elusive. Here, we found that the activity of hundreds of primate medial premotor cortices (MPCs; pre-supplementary motor area [preSMA] and supplementary motor area [SMA]) neurons show a strong periodic pattern that becomes evident when their responses are projected into a state space using dimensionality reduction analysis. Read More

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http://dx.doi.org/10.1371/journal.pbio.3000054DOI Listing

Response to: "Questioning the evidence for BCI-based communication in the complete locked-in state".

PLoS Biol 2019 Apr 8;17(4):e3000063. Epub 2019 Apr 8.

Institute of Medical Psychology and Behavioral Neurobiology, Eberhrd-Karls University of Tuebingen, Tuebingen, Germany.

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http://dx.doi.org/10.1371/journal.pbio.3000063DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6453359PMC
April 2019
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Questioning the evidence for BCI-based communication in the complete locked-in state.

Authors:
Martin Spüler

PLoS Biol 2019 Apr 8;17(4):e2004750. Epub 2019 Apr 8.

Department of Computer Engineering, Eberhard-Karls University Tübingen, Tübingen, Germany.

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http://dx.doi.org/10.1371/journal.pbio.2004750DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6453399PMC

Thought-based interaction: Same data, same methods, different results?

Authors:
Reinhold Scherer

PLoS Biol 2019 Apr 8;17(4):e3000190. Epub 2019 Apr 8.

Brain-Computer Interfaces and Neural Engineering Laboratory, School of Computer Science and Electronic Engineering, University of Essex, Wivenhoe Park, Colchester, United Kingdom.

Restoration of communication in people with complete motor paralysis-a condition called complete locked-in state (CLIS)-is one of the greatest challenges of brain-computer interface (BCI) research. New findings have recently been presented that bring us one step closer to this goal. However, the validity of the evidence has been questioned: independent reanalysis of the same data yielded significantly different results. Read More

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http://dx.doi.org/10.1371/journal.pbio.3000190DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6453358PMC
April 2019
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Exploring the potential of environment friendly silver nanoparticles for DNA interaction: Physicochemical approach.

J Photochem Photobiol B 2019 Mar 30;194:158-165. Epub 2019 Mar 30.

Nano-Bioconjugate Chemistry Lab, Cluster Innovation Centre, University of Delhi, Delhi, India. Electronic address:

Nanosilver, being the most prominent nanoproduct has diverse bio-medical applications and hence the effects associated with their exposure need to be investigated in detail. The interaction of metal nanoparticles with DNA has become a matter of interest, as their effect on structural integrity, synthesis and replication could be explored through it. Present work aims at the facile synthesis and characterization of spherical silver nanoparticles (AgNPs) using Epipremnum aureum leaves extract. Read More

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http://dx.doi.org/10.1016/j.jphotobiol.2019.03.022DOI Listing
March 2019
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Effects of photobiomodulation therapy on the extracellular matrix of human dental pulp cell sheets.

J Photochem Photobiol B 2019 Apr 1;194:149-157. Epub 2019 Apr 1.

Department of Restorative Dentistry, School of Dentistry, University of Sao Paulo, Brazil. Electronic address:

Photobiomodulation therapy (PBMT) and the cell sheet (CS) technology improve processes relevant to tissue regeneration. The aim of this study was to investigate the effects of different PBMT parameters on the architecture (histology), protein composition (Western blotting and immunohistochemistry) and ultrastructure [scanning electron microscopy (SEM) and transmission electron microscopy (TEM)] of the extracellular matrix (ECM) synthesized by CSs composed by human dental pulp stem cells (hDPSCs).

Methods: Thawed cells were recharacterized by the expression profile of the surface molecules of mesenchymal stem cells (MSCs) using flow cytometry. Read More

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http://dx.doi.org/10.1016/j.jphotobiol.2019.03.017DOI Listing

Mechanism of coralyne-mediated DNA photo-nicking process.

J Photochem Photobiol B 2019 Mar 21;194:140-148. Epub 2019 Mar 21.

Bio-Organic Division, Bhabha Atomic Research Centre, Mumbai 400085, India; Homi Bhabha National Institute, Training School Complex, Anushakti Nagar, Mumbai 400094, India.

Previously, we reported that coralyne and UVA combination sensitized a wide range of human carcinoma cells regardless of their p53 status. The coralyne induced photosensitization of cancer cells may be clinically attractive, as mutation in the p53 gene is prevalent in many types of tumors. Coralyne mediated photosensitization of cancer cells is attributable to its ability to cause extensive DNA single strand breaks (SSB). Read More

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http://dx.doi.org/10.1016/j.jphotobiol.2019.03.011DOI Listing
March 2019
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Quantum dots-based fluoroimmunoassay for anti-Zika virus IgG antibodies detection.

J Photochem Photobiol B 2019 Mar 27;194:135-139. Epub 2019 Mar 27.

Departamento de Biofísica e Radiobiologia, Universidade Federal de Pernambuco, Recife, PE, Brazil. Electronic address:

Zika virus (ZIKV) has been declared a public health emergency of international concern. ZIKV has been associated with some neurological disorders, and their long-term effects are not completely understood. The majority of the methods for ZIKV diagnosis are based on the detection of IgM antibodies, which are the first signs of immunological response. Read More

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http://dx.doi.org/10.1016/j.jphotobiol.2019.03.019DOI Listing

Effect of dose responses of hydrophilic aluminium (III) phthalocyanine chloride tetrasulphonate based photosensitizer on lung cancer cells.

J Photochem Photobiol B 2019 Mar 28;194:96-106. Epub 2019 Mar 28.

Laser Research Centre, Faculty of Health Sciences, University of Johannesburg, PO Box 17011, Johannesburg 2028, South Africa. Electronic address:

Photodynamic therapy (PDT) is a promising approach for the treatment of different types of cancer and has been brought into focus for its synergy, compatibility, repeatability, relatively inexpensive cost and it's typically more efficacious nature. Photosensitizers (PSs) play a major role in PDT and are the core of this specific therapy. Al (III) Phthalocyanine Chloride Tetra sulfonic Acid (AlPcSCl) PS is an aromatic macrocyclic metal-based compound that is synthetically derived. Read More

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http://dx.doi.org/10.1016/j.jphotobiol.2019.03.018DOI Listing

Preparation, and structural of new NiS-SiO and CrS-TiO nano-catalyst: Photocatalytic and antimicrobial studies.

J Photochem Photobiol B 2019 Mar 27;194:128-134. Epub 2019 Mar 27.

Engineering Department, Razak Faculty of Technology and Informatics, Universiti Teknologi, Malaysia, Jalan sultan Yahya Petra, 54100 Kuala Lumpur.

NiS-SiO and CrS-TiO synthesized by Ultrasound-Microwave method was tested for the photo-degradation of methyl red as azo dye under ultraviolet (UV) light. The structure and morphology of the synthesized materials were examined through scanning electron microscopy, X-ray diffraction and photoelectron spectroscopy, energy-dispersive spectroscopy, dynamic light scattering and the band gap energy differences were determined through diffuse reflectance spectroscopy (DRS). The crystallite size and band gap values of SiO, TiO, NiS-SiO and CrS-TiO-1 were obtained from XRD and UV-vis DRS analysis and found insignificant 44. Read More

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http://dx.doi.org/10.1016/j.jphotobiol.2019.03.016DOI Listing
March 2019
2.960 Impact Factor

Bio-engineering and cellular imaging of silver nanoparticles as weaponry against multidrug resistant human pathogens.

J Photochem Photobiol B 2019 Mar 28;194:119-127. Epub 2019 Mar 28.

Faculty of Bio & Chemical Engineering, Sathyabama Institute of Science and Technology, Rajiv Gandhi Road, Chennai, Tamilnadu 600119, India.

'Go green' has also been implied to nanotechnology by harbouring eco-benign principle for a cleaner production of silver nanoparticles (AgNPs). This was achieved using a nitrate reducing Bacillus subtilis L1 (KT266579.1) inhabiting rhizosphere soil under optimized laboratory conditions, highlighting on its antibacterial modus operandi. Read More

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http://dx.doi.org/10.1016/j.jphotobiol.2019.03.021DOI Listing
March 2019
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Altered metabolic and hormonal responses in male rats exposed to acute bright light-at-night associated with global DNA hypo-methylation.

J Photochem Photobiol B 2019 Mar 28;194:107-118. Epub 2019 Mar 28.

The Israeli Center for Interdisciplinary Research in Chronobiology, University of Haifa, Mount Carmel, Haifa 3498838, Israel. Electronic address:

The association between light pollution and disruption of daily rhythms, metabolic and hormonal disorders, as well as cancer progression is well-recognized. These adverse effects could be due to nocturnal melatonin suppression. The signaling pathway by which light pollution affects metabolism and endocrine responses is unclear. Read More

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http://dx.doi.org/10.1016/j.jphotobiol.2019.03.020DOI Listing
March 2019
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Signaling by adenosine receptors-Homeostatic or allostatic control?

Authors:
Rodrigo A Cunha

PLoS Biol 2019 Apr 5;17(4):e3000213. Epub 2019 Apr 5.

CNC-Center for Neuroscience and Cell Biology, University of Coimbra, Coimbra, Portugal.

Adenosine modulation is considered both a paracrine signal coordinating different cells in a tissue and a stress signal. Both functions are ensured by 4 types of adenosine receptors (ARs), which have been studied individually. Mice with knockout of all ARs (quad-AR-KO) now allow enquiring the overall function of the adenosine modulation system. Read More

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http://dx.doi.org/10.1371/journal.pbio.3000213DOI Listing
April 2019
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