10,664 results match your criteria Biologics: Targets & Therapy[Journal]


Evaluation of the Anti-Tumor Activity of the Humanized Monoclonal Antibody NEO-201 in Preclinical Models of Ovarian Cancer.

Front Oncol 2020 19;10:805. Epub 2020 Jun 19.

Women's Malignancy Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, United States.

Despite high initial response rates with cytoreductive surgery, conventional chemotherapy and the incorporation of biologic agents, ovarian cancer patients often relapse and die from their disease. New approaches are needed to improve patient outcomes. This study was designed to evaluate the antitumor activity of NEO-201 monoclonal antibody (mAb) in preclinical models of ovarian cancer where the NEO-201 target is highly expressed. Read More

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http://dx.doi.org/10.3389/fonc.2020.00805DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7318110PMC

Clinical Practice of Adalimumab and Infliximab Biosimilar Treatment in Adult Patients With Crohn's Disease.

Inflamm Bowel Dis 2020 Jul 7. Epub 2020 Jul 7.

IBD Center, Department of Gastroenterology, Humanitas Clinical and Research Center, Milan, Italy.

The introduction of tumor necrosis factor (TNF) inhibitors has significantly changed the treatment landscape in Crohn's disease (CD). The overall therapeutic achievements with TNF inhibitors such as infliximab, adalimumab, and certolizumab pegol paved the way to push the boundaries of treatment goals beyond symptomatic relief and toward cessation of objective signs of inflammation, including endoscopic remission. Even though these agents are widely used for the treatment of moderate to severe CD, heterogeneity still exists in translating evidence-based guidelines on the use of anti-TNF agents into actual treatment algorithms in CD. Read More

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http://dx.doi.org/10.1093/ibd/izaa078DOI Listing

EMA and EFSA Joint Scientific Opinion on measures to reduce the need to use antimicrobial agents in animal husbandry in the European Union, and the resulting impacts on food safety (RONAFA).

EFSA J 2017 Jan 24;15(1):e04666. Epub 2017 Jan 24.

EFSA and EMA have jointly reviewed measures taken in the EU to reduce the need for and use of antimicrobials in food-producing animals, and the resultant impacts on antimicrobial resistance (AMR). Reduction strategies have been implemented successfully in some Member States. Such strategies include national reduction targets, benchmarking of antimicrobial use, controls on prescribing and restrictions on use of specific critically important antimicrobials, together with improvements to animal husbandry and disease prevention and control measures. Read More

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http://dx.doi.org/10.2903/j.efsa.2017.4666DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7010070PMC
January 2017

New insights in enumeration methodologies of probiotic cells in finished products.

J Microbiol Methods 2020 Jul 1:105993. Epub 2020 Jul 1.

Probiotical Research S.r.l., Enrico Mattei, 3- 28100 Novara, Italy. Electronic address:

The number of bacterial cells is currently recognized as the most important parameter for the efficacy and quality of finished probiotic or live biotherapeutic products (LBP). Cell enumeration is generally performed by culture-dependent methodologies like plate count (PC). These techniques are able to reveal the number of viable cells able to replicate and generate a colony. Read More

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http://dx.doi.org/10.1016/j.mimet.2020.105993DOI Listing

Biological activity of mistletoe: in vitro and in vivo studies and mechanisms of action.

Arch Pharm Res 2020 Jun 3;43(6):593-629. Epub 2020 Jul 3.

Department of Spectrometric Methods, National Medicines Institute, Chełmska 30/34, 00-725, Warsaw, Poland.

Mistletoe has been used as treatment of many diseases in traditional and folk medicine. To date, anticancer, immunomodulatory, cardiac, antidiabetic, hepatoprotective, neuropharmacological, antibacterial and antifungal properties of mistletoe extracts have been studied the most. In this review, we summarized in vitro and in vivo studies on the pharmacological activity of Viscum species. Read More

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http://dx.doi.org/10.1007/s12272-020-01247-wDOI Listing

Yohimbine as a Starting Point to Access Diverse Natural Product-Like Agents with Re-programmed Activities against Cancer-Relevant GPCR Targets.

Bioorg Med Chem 2020 Jul 7;28(14):115546. Epub 2020 May 7.

University of Florida, Department of Medicinal Chemistry, Center for Natural Products, Drug Discovery and Development (CNPD3), College of Pharmacy, 1345 Center Dr., Gainesville, FL 32610, United States. Electronic address:

G protein-coupled receptors (GPCRs) constitute the largest protein superfamily in the human genome. GPCRs play key roles in mediating a wide variety of physiological events including proliferation and cancer metastasis. Given the major roles that GPCRs play in mediating cancer growth, they present promising targets for small molecule therapeutics. Read More

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http://dx.doi.org/10.1016/j.bmc.2020.115546DOI Listing

Beyond the Paclitaxel and Vinca Alkaloids: Next Generation of Plant-Derived Microtubule-Targeting Agents with Potential Anticancer Activity.

Cancers (Basel) 2020 Jun 29;12(7). Epub 2020 Jun 29.

Division of Oncology Research, Mayo Clinic, Rochester, MN 55905, USA.

Plants are an important source of chemically diverse natural products that target microtubules, one of the most successful targets in cancer therapy. Colchicine, paclitaxel, and vinca alkaloids are the earliest plant-derived microtubule-targeting agents (MTAs), and paclitaxel and vinca alkaloids are currently important drugs used in the treatment of cancer. Several additional plant-derived compounds that act on microtubules with improved anticancer activity are at varying stages of development. Read More

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http://dx.doi.org/10.3390/cancers12071721DOI Listing

MicroRNA-92a Targets SERTAD3 and Regulates the Growth, Invasion, and Migration of Prostate Cancer Cells via the P53 Pathway.

Onco Targets Ther 2020 12;13:5495-5514. Epub 2020 Jun 12.

Department of Anatomy, School of Basic Medical Sciences, Guizhou Medical University, Guizhou, People's Republic of China.

Background: The miR-17-92 cluster, consisting of six mature miRNAs including miR-17, miR-18a, miR-19a, miR-19b, miR-20a, and miR-92a, plays a key role in the tumorigenesis and development of various cancers. The dysregulation of the cluster correlates with the biological mechanism of tumor growth and metastasis in vivo. However, the relationship between miR-17-92 cluster and malignancy of prostate cancer remains unclear, and its regulatory mechanism is worth investigating for controlling the proliferation and invasion of prostate cancer. Read More

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http://dx.doi.org/10.2147/OTT.S249168DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7298502PMC

Deciphering Alzheimer's disease: predicting new therapeutic strategies via improved understanding of biology and pathogenesis.

Expert Opin Ther Targets 2020 Jun 30. Epub 2020 Jun 30.

Department of Psychiatry and Behavioral Neuroscience, Saint Louis University School of Medicine, Saint Louis , Missouri, USA.

Introduction: There is no cure for Alzheimer's disease (AD). One explanation may pertain to the need to intervene as early as possible upstream from the accumulation of β-amyloid plaques and tau tangles.

Areas Covered: A PUBMED literature search was completed to review the biological or pathological changes at the basis of disease initiation; this includes neuroinflammation, oxidative stress, microbiome changes and glymphatic system dysfunction. Read More

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http://dx.doi.org/10.1080/14728222.2020.1790530DOI Listing

A simple mesoporous silica nanoparticle-based fluorescence aptasensor for the detection of zearalenone in grain and cereal products.

Anal Bioanal Chem 2020 Jun 30. Epub 2020 Jun 30.

College of Food Science, Southwest University, No.2 Tiansheng Road, Beibei District, Chongqing, 400715, China.

Zearalenone (ZEN) is a type of estrogenic mycotoxin commonly occurring in cereals. The aim of this study was to design a simple, rapid, inexpensive and ultrasensitive fluorescence assay for the determination of ZEN. Here, amino-modified mesoporous silica nanoparticles (MSNs-NH) were synthesized to be the positive charge-rich reactor. Read More

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http://dx.doi.org/10.1007/s00216-020-02778-3DOI Listing

Virtual Screening Identifies Chebulagic Acid as an Inhibitor of the M2(S31N) Viral Ion Channel and Influenza A Virus.

Molecules 2020 Jun 24;25(12). Epub 2020 Jun 24.

Faculty of Health Sciences, Simon Fraser University, Burnaby, BC V5A 1S6, Canada.

The increasing prevalence of drug-resistant influenza viruses emphasizes the need for new antiviral countermeasures. The M2 protein of influenza A is a proton-gated, proton-selective ion channel, which is essential for influenza replication and an established antiviral target. However, all currently circulating influenza A virus strains are now resistant to licensed M2-targeting adamantane drugs, primarily due to the widespread prevalence of an M2 variant encoding a serine to asparagine 31 mutation (S31N). Read More

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http://dx.doi.org/10.3390/molecules25122903DOI Listing

Clinical trials and novel therapeutics in Dermatomyositis.

Expert Opin Emerg Drugs 2020 Jun 29. Epub 2020 Jun 29.

Department of Medicine, Rheumatology and Clinical Immunology, University of Pittsburgh , Pittsburgh, PA.

Introduction: Currently, there are no proven drugs that are FDA approved for the treatment of dermatomyositis (DM), even though multiple clinical trials are ongoing to evaluate safety and efficacy of novel therapeutics in DM. The purpose of this review is to highlight the biological plausibility, existing clinical evidence as well as completed and ongoing clinical trials for various drugs in pipeline for development for use in dermatomyositis.

Areas Covered: The drugs with the strongest evidence have been included in this review with a focus on the mechanism of their action pertaining to the disease process, clinical studies including completed and ongoing trials. Read More

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http://dx.doi.org/10.1080/14728214.2020.1787985DOI Listing

Anti-alarmins in asthma: targeting the airway epithelium with next-generation biologics.

Eur Respir J 2020 Jun 25. Epub 2020 Jun 25.

Department of Respiratory Medicine, Amsterdam University Medical Centre, University of Amsterdam, Amsterdam, Netherlands.

Monoclonal antibody therapies have significantly improved treatment outcomes for patients with severe asthma; however, a significant disease burden remains. Available biologic treatments, including anti-immunoglobulin (Ig) E, anti-interleukin (IL)-5, anti-IL-5Rα and anti-IL-4Rα, reduce exacerbation rates in study populations by approximately 50% only. Furthermore, there are currently no effective treatments for patients with severe, type 2 (T2)-low asthma. Read More

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http://dx.doi.org/10.1183/13993003.00260-2020DOI Listing

Modelling Spatial Scales of Dose Deposition and Radiolysis Products from Gold Nanoparticle Sensitisation of Proton Therapy in A Cell: From Intracellular Structures to Adjacent Cells.

Int J Mol Sci 2020 Jun 22;21(12). Epub 2020 Jun 22.

Department of Physics, University of Adelaide, Adelaide, SA 5005, Australia.

Gold nanoparticle (GNP) enhanced proton therapy is a promising treatment concept offering increased therapeutic effect. It has been demonstrated in experiments which provided indications that reactive species play a major role. Simulations of the radiolysis yield from GNPs within a cell model were performed using the Geant4 toolkit. Read More

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http://dx.doi.org/10.3390/ijms21124431DOI Listing

Improving Precision and Power in Randomized Trials for COVID-19 Treatments Using Covariate Adjustment, for Binary, Ordinal, and Time-to-Event Outcomes.

medRxiv 2020 Jun 11. Epub 2020 Jun 11.

Time is of the essence in evaluating potential drugs and biologics for the treatment and prevention of COVID-19. There are currently over 400 clinical trials (phase 2 and 3) of treatments for COVID-19 registered on clinicaltrials.gov. Read More

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http://dx.doi.org/10.1101/2020.04.19.20069922DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7302221PMC

Investigation of syphilis immunology and Treponema pallidum subsp. pallidum biology to improve clinical management and design a broadly protective vaccine: study protocol.

BMC Infect Dis 2020 Jun 23;20(1):444. Epub 2020 Jun 23.

Division of Infectious Diseases, David Geffen School of Medicine, University of California Los Angeles, 10833 Le Conte Ave, Los Angeles, CA, 90095, USA.

Background: The syphilis epidemic continues to cause substantial morbidity and mortality worldwide, particularly in low- and middle-income countries, despite several recent disease control initiatives. Though our understanding of the pathogenesis of this disease and the biology of the syphilis agent, Treponema pallidum subsp. pallidum has improved over the last two decades, further research is necessary to improve clinical diagnosis and disease management protocols. Read More

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http://dx.doi.org/10.1186/s12879-020-05141-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7309211PMC

Cyclic Imines in Ugi and Ugi-Type Reactions.

ACS Comb Sci 2020 Jun 23. Epub 2020 Jun 23.

Ugi four-component reactions (U-4CRs) are widely recognized as being highly efficient for the synthesis of pseudopeptides. However, the products of these reactions are not so interesting as drug candidates because they are not conformationally restricted enough for a potent interaction with biological targets. One possible way to overcome this problem is to replace amine and oxo components in the U-4CRs with cyclic imines in so-called Joullié-Ugi three-component reactions (JU-3CRs). Read More

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http://dx.doi.org/10.1021/acscombsci.0c00046DOI Listing

Molecular mechanisms of underlying genetic factors and associated mutations for drug resistance in .

Emerg Microbes Infect 2020 Jun 23:1-32. Epub 2020 Jun 23.

Division of Public Health and Research, ICMR-Regional Medical Research Centre, Bhubaneswar, Odisha, India.

Nowadays, drug-resistant tuberculosis (DR-TB) and co-infected tuberculosis (CI-TB) strains are the leading cause for the enhancement of long-mortality and unpredicted mortality rates from this ghoulish acid fast-bacterium infection, globally. Unfortunately, the lack/ample lethargic towards the development of compelling anti-regimens with a large scale prevalence rate is a great challenge towards control of the pandemic situation. Indeed, the recent improvement in genomic studies for early diagnosis and understanding the mechanisms of drug resistance, as well as the identification of newer drug targets is quite remarkable and promising. Read More

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http://dx.doi.org/10.1080/22221751.2020.1785334DOI Listing

Dr AFC: drug repositioning through anti-fibrosis characteristic.

Brief Bioinform 2020 Jun 22. Epub 2020 Jun 22.

Tongji University, Shanghai, China.

Fibrosis is a key component in the pathogenic mechanism of a variety of diseases. These diseases involving fibrosis may share common mechanisms and therapeutic targets, and therefore common intervention strategies and medicines may be applicable for these diseases. For this reason, deliberately introducing anti-fibrosis characteristics into predictive modeling may lead to more success in drug repositioning. Read More

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http://dx.doi.org/10.1093/bib/bbaa115DOI Listing

COVID-19 Coronavirus spike protein analysis for synthetic vaccines, a peptidomimetic antagonist, and therapeutic drugs, and analysis of a proposed achilles' heel conserved region to minimize probability of escape mutations and drug resistance.

Authors:
B Robson

Comput Biol Med 2020 06 11;121:103749. Epub 2020 Apr 11.

Ingine Inc. Cleveland Ohio USA, The Dirac Foundation, Oxfordshire, UK. Electronic address:

This paper continues a recent study of the spike protein sequence of the COVID-19 virus (SARS-CoV-2). It is also in part an introductory review to relevant computational techniques for tackling viral threats, using COVID-19 as an example. Q-UEL tools for facilitating access to knowledge and bioinformatics tools were again used for efficiency, but the focus in this paper is even more on the virus. Read More

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http://dx.doi.org/10.1016/j.compbiomed.2020.103749DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7151553PMC

Recent Trends in Drug Design and Discovery.

Curr Top Med Chem 2020 Jun 22. Epub 2020 Jun 22.

Department of Biotechnology, Bhupat and Jyoti Mehta School of Biosciences, Indian Institute of Technology (IIT) Madras, Chennai - 600036. India.

Structure-based drug design is a wide area of identification of selective inhibitors of a target of interest. From the time of the availability of three dimensional structure of the drug targets, mostly, the proteins, many computational methods had emerged to address the challenges associated with drug design process. Particularly, drug-likeness, druggability of the target protein, specificity, off-target binding, etc. Read More

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http://dx.doi.org/10.2174/1568026620666200622150003DOI Listing

Review of monoclonal antibody therapies in asthma and allergic diseases - a new paradigm for precision medicine.

Asian Pac J Allergy Immunol 2020 Jun;38(2):78-90

Faculty of Life Sciences & Medicine, School of Immunology & Microbial Sciences, Asthma UK Centre in Allergic Mechanisms of Asthma, King's College London, London, UK.

Background: Elucidation of the critical immune pathways involved in allergic inflammation has identified, apart from IgE, therapeutic targets in the cytokine network suitable for intervention by biological therapies.

Objective: The drugs that target the cytokine networks pertinent to asthma and allergic diseases are reviewed and some illustrative case histories presented. The overview proposes a framework to use when deciding which monoclonal antibody (mAb) to select for treatment of severe asthma based on total IgE concentration, peripheral blood eosinophil count, induced sputum analysis and measurement of fractional exhaled nitric oxide (FENO). Read More

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http://dx.doi.org/10.12932/AP-020220-0752DOI Listing

Characterization of exosome-like vesicles derived from Taenia pisiformis cysticercus and their immunoregulatory role on macrophages.

Parasit Vectors 2020 Jun 19;13(1):318. Epub 2020 Jun 19.

State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, Gansu Province, People's Republic of China.

Background: Taenia pisiformis is one of the most common intestinal parasites in canines, and leads to serious economic losses in the rabbit breeding industry. Exosome-like vesicles from parasites play crucial roles in host-parasite interactions by transferring cargo from parasites to host cells and by modulating host immunological response through inducing production of host-derived cytokines. Nevertheless, the mechanism by which exosome-like vesicles from T. Read More

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http://dx.doi.org/10.1186/s13071-020-04186-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7304098PMC

Antinociceptive Activity of Chemical Components of Essential Oils That Involves Docking Studies: A Review.

Front Pharmacol 2020 29;11:777. Epub 2020 May 29.

Psychopharmacology Laboratory, Institute of Drugs and Medicines Research, Federal University of Paraíba, João Pessoa, Brazil.

Introduction: Pain is considered an unpleasant sensory and emotional experience, being considered as one of the most important causes of human suffering. Computational chemistry associated with bioinformatics has stood out in the process of developing new drugs, through natural products, to manage this condition.

Objective: To analyze, through literature data, recent molecular coupling studies on the antinociceptive activity of essential oils and monoterpenes. Read More

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http://dx.doi.org/10.3389/fphar.2020.00777DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7272657PMC

Protoflavone-Chalcone Hybrids Exhibit Enhanced Antitumor Action through Modulating Redox Balance, Depolarizing the Mitochondrial Membrane, and Inhibiting ATR-Dependent Signaling.

Antioxidants (Basel) 2020 Jun 12;9(6). Epub 2020 Jun 12.

Institute of Pharmacognosy, Interdisciplinary Excellence Centre, University of Szeged, Eötvös str. 6, H-6720 Szeged, Hungary.

Hybrid compounds combine fragments with complementary targets to achieve a common pharmacological goal. This approach represents an increasingly popular strategy for drug discovery. In this work, we aimed to design antitumor hybrid compounds based on an inhibitor of ataxia-telangiectasia and Rad3-related protein (ATR)-dependent signaling, protoapigenone, and a pro-oxidant ferrocene or chalcone fragment. Read More

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http://dx.doi.org/10.3390/antiox9060519DOI Listing

Blacks/African Americans with Pediatric Crohn's Disease Report Less Anxiety and Fatigue than Whites.

J Pediatr 2020 Jun 13. Epub 2020 Jun 13.

Departments of Pediatrics. Electronic address:

Objectives: To compare patient reported outcomes in Blacks/African Americans with Whites participating in IBD Partners Kids & Teens, in order to identify possible racial health care disparities in pediatric inflammatory bowel disease (IBD) as future targets for improvement.

Study Design: Cross-sectional analysis comparing patient reported outcomes in Black/African American with White patients, age 9-18 years, with IBD participating in the IBD Partners Kids & Teens cohort from 8/2013 to 4/2018. Secondary outcomes included number of IBD-related hospitalizations and surgeries, current medication use, and disease activity. Read More

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http://dx.doi.org/10.1016/j.jpeds.2020.05.042DOI Listing

Characterization of concurrent target suppression by JNJ-61178104, a bispecific antibody against human tumor necrosis factor and interleukin-17A.

MAbs 2020 Jan-Dec;12(1):1770018

Clinical Pharmacology and Pharmacometrics, Janssen R&D , Spring House, PA, USA.

Tumor necrosis factor (TNF) and interleukin (IL)-17A are pleiotropic cytokines implicated in the pathogenesis of several autoimmune diseases including rheumatoid arthritis (RA) and psoriatic arthritis (PsA). JNJ-61178104 is a novel human anti-TNF and anti-IL-17A monovalent, bispecific antibody that binds to both human TNF and human IL-17A with high affinities and blocks the binding of TNF and IL-17A to their receptors . JNJ-61178104 also potently neutralizes TNF and IL-17A-mediated downstream effects in multiple cell-based assays. Read More

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http://dx.doi.org/10.1080/19420862.2020.1770018DOI Listing

Targeting eukaryotic proteases for natural products-based drug development.

Nat Prod Rep 2020 Jun;37(6):827-860

Department of Medicinal Chemistry, Center for Natural Products, Drug Discovery and Development (CNPD3), University of Florida, 1345 Center Drive, Gainesville, Florida 32610, USA.

Covering: up to April 2020 Proteases are involved in the regulation of many physiological processes. Their overexpression and dysregulated activity are linked to diseases such as hypertension, diabetes, viral infections, blood clotting disorders, respiratory diseases, and cancer. Therefore, they represent an important class of therapeutic targets. Read More

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http://dx.doi.org/10.1039/c9np00060gDOI Listing

The serum protein transthyretin as a platform for dimerization and tetramerization of antibodies and Fab fragments to enable target clustering.

J Biol Chem 2020 Jun 9. Epub 2020 Jun 9.

Amgen Inc., United States.

Transthyretin (TTR) is an abundant homo-tetrameric serum protein and was selected here for engineering higher-valency molecules because its compact size, simple structure, and natural propensity to tetramerize.   To demonstrate this utility, we fused TTR to the C terminus of conatumumab, an antibody that targets tumor necrosis factor-related apoptosis-inducing ligand receptor 2 (TRAIL-R2), as heavy chains to form antibody dimers and Fab heavy chains to form Fab tetramers.  Moreover, we used constant heavy domain 3 (CH3) heterodimerization substitutions to create TTR-mediated conatumumab tetramers. Read More

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http://dx.doi.org/10.1074/jbc.RA120.013135DOI Listing

Biological and catalytic functions of sirtuin 6 as targets for small-molecule modulators.

J Biol Chem 2020 06 9. Epub 2020 Jun 9.

University of Wisconsin, United States.

Sirtuin 6 (SIRT6) is a nuclear NAD-dependent deacetylase of histone H3 that regulates genome stability and gene expression. However, non-histone substrates and additional catalytic activities of SIRT6, including long-chain deacylation and mono-ADP-ribosylation of other proteins have also been reported, but many of these non-canonical roles remain enigmatic. Genetic studies have revealed critical homeostatic cellular functions of SIRT6, underscoring the need to better understand which catalytic functions and molecular pathways are driving SIRT6-associated phenotypes. Read More

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http://dx.doi.org/10.1074/jbc.REV120.011438DOI Listing

Oleamide in Ipomoea and Dillenia species and inflammatory activity investigated through ion channel inhibition.

Curr Pharm Biotechnol 2020 Jun 7. Epub 2020 Jun 7.

Toxicology and Pharmacology, University of Leuven (KU Leuven), Leuven. Belgium.

Background: Oleamide is an essential substance for human health. So, the plants with high oleamide content are great sources for health care products.

Objective: This study is conducted to investigate the quality of oleamide in plants and test the bioactivity in the selected two studied species. Read More

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http://dx.doi.org/10.2174/1389201021666200607185250DOI Listing

Antibacterial mechanism of rhodomyrtone involves the disruption of nucleoid segregation checkpoint in Streptococcus suis.

AMB Express 2020 Jun 8;10(1):110. Epub 2020 Jun 8.

Graduate Program in Biomedical Sciences, Faculty of Allied Health Sciences, Thammasat University, Khlong Nueng, Khlong Luamg, Pathumthani, 12120, Thailand.

Rhodomyrtone has been recently demonstrated to possess a novel antibiotic mechanism of action against Gram-positive bacteria which involved the multiple targets, resulting in the interference of several bacterial biological processes including the cell division. The present study aims to closely look at the downstream effect of rhodomyrtone treatment on nucleoid segregation in Streptococcus suis, an important zoonotic pathogen. The minimum inhibition concentration (MIC) and the minimum bactericidal concentration (MBC) values of rhodomyrtone against the recombinant S. Read More

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http://dx.doi.org/10.1186/s13568-020-01047-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7280372PMC

Sequence analysis of SARS-CoV-2 genome reveals features important for vaccine design.

bioRxiv 2020 Mar 31. Epub 2020 Mar 31.

Center for Biologics Evaluation and Research, Office of Tissues and Advanced Therapies, Division of Plasma Protein Therapeutics, Food and Drug Administration, Silver Spring, MD, USA.

As the SARS-CoV-2 pandemic is rapidly progressing, the need for the development of an effective vaccine is critical. A promising approach for vaccine development is to generate, through codon pair deoptimization, an attenuated virus. This approach carries the advantage that it only requires limited knowledge specific to the virus in question, other than its genome sequence. Read More

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http://dx.doi.org/10.1101/2020.03.30.016832DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7217226PMC

Learning from the Past: Possible Urgent Prevention and Treatment Options for Severe Acute Respiratory Infections Caused by 2019-nCoV.

ChemRxiv 2020 Jan 27. Epub 2020 Jan 27.

With the current trajectory of the 2019-nCoV outbreak unknown, public health and medicinal measures will both be needed to contain spreading of the virus and to optimize patient outcomes. While little is known about the virus, an examination of the genome sequence shows strong homology with its more well-studied cousin, SARS-CoV. The spike protein used for host cell infection shows key nonsynonymous mutations which may hamper efficacy of previously developed therapeutics but remains a viable target for the development of biologics and macrocyclic peptides. Read More

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http://dx.doi.org/10.26434/chemrxiv.11728983DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7251965PMC
January 2020

The roles and applications of chaotropes and kosmotropes in industrial fermentation processes.

Authors:
David J Timson

World J Microbiol Biotechnol 2020 Jun 8;36(6):89. Epub 2020 Jun 8.

School of Pharmacy and Biomolecular Sciences, University of Brighton, Huxley Building, Lewes Road, Brighton, BN2 4GJ, UK.

Chaotropicity has long been recognised as a property of some compounds. Chaotropes tend to disrupt non-covalent interactions in biological macromolecules (e.g. Read More

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http://dx.doi.org/10.1007/s11274-020-02865-8DOI Listing

Analysis of the molecular mechanism of Pudilan (PDL) treatment for COVID-19 by network pharmacology tools.

Biomed Pharmacother 2020 Aug 30;128:110316. Epub 2020 May 30.

Medical College, Qingdao University, Qingdao 266071, PR China. Electronic address:

Background: Pudilan (PDL), a four-herb prescription with the traditional function of heat-clearing and detoxifying, has been clinically used as an anti-SARS-CoV-2 infectory agent in China. PDL might also have therapeutic potentials for COVID-19 while the underlying mechanisms remain to be clarified.

Methods: We used network pharmacology analysis and selected 68 co-targeted genes/proteins as targets of both PDL and COVID-19. Read More

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http://dx.doi.org/10.1016/j.biopha.2020.110316DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7260557PMC
August 2020
2.023 Impact Factor

Significance of and Challenges in Regulating Endocrine Disruptors - How Regulators and Industry can Conquer?

Endocr Metab Immune Disord Drug Targets 2020 Jun 6. Epub 2020 Jun 6.

Department of Pharmaceutical Sciences, Maharshi Dayanand University, Rohtak -124001, Haryana. India.

Background: Endocrine disruptors (EDs) disrupt the standard operation of the endocrine systems, resulting in untoward effects. EDs have gained extensive consideration due to their severe adverse impacts on public and wildlife health. A variety of compounds from both natural and synthetic origin may cause endocrine disruptions. Read More

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http://dx.doi.org/10.2174/1871530320666200606225104DOI Listing

Repurposing Modular Polyketide Synthases and Non-ribosomal Peptide Synthetases for Novel Chemical Biosynthesis.

Front Mol Biosci 2020 15;7:87. Epub 2020 May 15.

Systems and Synthetic Biology Laboratory, Department of Biological Sciences and KI for the BioCentury, Korea Advanced Institute of Science and Technology, Daejeon, South Korea.

In nature, various enzymes govern diverse biochemical reactions through their specific three-dimensional structures, which have been harnessed to produce many useful bioactive compounds including clinical agents and commodity chemicals. Polyketide synthases (PKSs) and non-ribosomal peptide synthetases (NRPSs) are particularly unique multifunctional enzymes that display modular organization. Individual modules incorporate their own specific substrates and collaborate to assemble complex polyketides or non-ribosomal polypeptides in a linear fashion. Read More

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http://dx.doi.org/10.3389/fmolb.2020.00087DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7242659PMC

Interactions of antithrombotic herbal medicines with Western cardiovascular drugs.

Pharmacol Res 2020 Jun 1;159:104963. Epub 2020 Jun 1.

State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao SAR, 999078, China. Electronic address:

Thrombotic events act as a critical factor that interferes with Cardiovascular Diseases (CVDs), and antithrombotic herbal medicine is a long-standing controversial issue. Although a dispute is involved in their clinical application, all parties unanimously agree that herbal products have been widely used in folk medicine, and their interactions with conventional drugs are of high concern. This study aims to investigate how antithrombotic herbal medicines interact with Western cardiovascular drugs on the molecular level by taking an example of the most frequently used herbal pair, Danshen-Chuanxiong (DS-CX), and to discover more scientific evidence on their potential herb-drug interactions. Read More

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http://dx.doi.org/10.1016/j.phrs.2020.104963DOI Listing

Peripheral blood transcriptome identifies high-risk benign and malignant breast lesions.

PLoS One 2020 4;15(6):e0233713. Epub 2020 Jun 4.

Huaxia Bangfu Technology Incorporated, Beijing, People's Republic of China.

Background: Peripheral blood transcriptome profiling is a potentially important tool for disease detection. We utilize this technique in a case-control study to identify candidate transcriptomic biomarkers able to differentiate women with breast lesions from normal controls.

Methods: Whole blood samples were collected from 50 women with high-risk breast lesions, 57 with breast cancers and 44 controls (151 samples). Read More

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0233713PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7272048PMC

[Pharmacovigilance study on drug-induced cardiac injury during treatment of COVID-19].

Zhongguo Zhong Yao Za Zhi 2020 May;45(10):2275-2286

School of Chinese Materia Medica, Beijing University of Chinese Medicine Beijing 102488, China.

Because coronavirus disease 2019(COVID-19) is highly contagious and serious, it has posed a major threat to public health worldwide. The curative effects of integrated traditional Chinese medicine and Western medicine in the treatment of COVID-19 have been widely recognized and confirmed. However, medical workers shall pay attention to drug-induced heart injury in clinical application. Read More

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http://dx.doi.org/10.19540/j.cnki.cjcmm.20200322.503DOI Listing

[Mechanism of Xuanfei Baidu Tang in treatment of COVID-19 based on network pharmacology].

Zhongguo Zhong Yao Za Zhi 2020 May;45(10):2249-2256

State Key Laboratory of Component Chinese Medicine, Tianjin University of Traditional Chinese Medicine Tianjin 300193, China.

The study aimed to investigate the multi-constituent, multi-target mechanism of Xuanfei Baidu Tang(XFBD) in the treatment of coronavirus disease 2019(COVID-19), through exploring the main ingredients and effective targets of XFBD, as well as analyzing the correlation between XFBD targets and COVID-19. The compounds of each herb in XFBD were collected from TCM-PTD, ETCM, TCMSP and SymMap database. Next, the information of meridian tropisms was collected from Chinese Pharmacopoeia(2015 edition), and the target information of the major constituents of XFBD were obtained from TCM-PTD, ETCM, TCMSP and TargetNet database. Read More

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http://dx.doi.org/10.19540/j.cnki.cjcmm.20200325.401DOI Listing

SARS-CoV-2 SPIKE PROTEIN: an optimal immunological target for vaccines.

J Transl Med 2020 06 3;18(1):222. Epub 2020 Jun 3.

Takis s.r.l, Via di Castel Romano 100, 00128, Rome, Italy.

COVID-19 has rapidly spread all over the world, progressing into a pandemic. This situation has urgently impelled many companies and public research institutes to concentrate their efforts on research for effective therapeutics. Here, we outline the strategies and targets currently adopted in developing a vaccine against SARS-CoV-2. Read More

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http://dx.doi.org/10.1186/s12967-020-02392-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7268185PMC

[Intervention mechanism of Qingwen Baidu Yin on cytokine storm based on network pharmacology].

Zhongguo Zhong Yao Za Zhi 2020 Apr;45(7):1499-1508

Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China.

The aim of this paper was to explore the intervention mechanism of Qingwen Baidu Yin in cytokine storm based on network pharmacology. TCMSP and TCMIP V2.0 server were used to predict all chemical components and action targets of Qingwen Baidu Yin. Read More

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http://dx.doi.org/10.19540/j.cnki.cjcmm.20200305.401DOI Listing

[Exploration on scientific connotation of TCM syndromes and recommended prescriptions against COVID-19 based on TCMTP V2.0].

Zhongguo Zhong Yao Za Zhi 2020 Apr;45(7):1488-1498

Shaanxi Institute of International Trade & Commerce Xi'an 712046, China.

Coronavirus disease 2019(COVID-19) has attracted great attentions from the whole world. Traditional Chinese medicine(TCM) has been widely used and shown satisfying efficacies in treating all stages of COVID-19. In this study, the molecular interaction networks of different stages of COVID-19(the early, severe, critical and recovery stage) were constructed using the links among symptoms-related genes collected from TCMIP V2. Read More

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http://dx.doi.org/10.19540/j.cnki.cjcmm.20200229.401DOI Listing

Extracts and Marine Algae Polysaccharides in Therapy and Prevention of Inflammatory Diseases of the Intestine.

Mar Drugs 2020 May 31;18(6). Epub 2020 May 31.

G.B. Elyakov Pacific Institute of Bioorganic Chemistry, FEB RAS, Vladivostok 690022, Russia.

Inflammatory bowel disease (IBD) is a serious public health problem worldwide. Current therapeutic strategies that use anti-inflammatory drugs, immunosuppressants, and biological treatments are often ineffective and have adverse health effects. In this regard, the use of natural compounds aimed at key pathogenic therapeutic targets in IBD attracts universal attention. Read More

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http://dx.doi.org/10.3390/md18060289DOI Listing

Dithranol targets keratinocytes, their crosstalk with neutrophils and inhibits the IL-36 inflammatory loop in psoriasis.

Elife 2020 06 2;9. Epub 2020 Jun 2.

Department of Dermatology, Medical University of Graz, Graz, Austria.

Despite the introduction of biologics, topical dithranol (anthralin) has remained one of the most effective anti-psoriatic agents. Serial biopsies from human psoriatic lesions and both the c-Jun/JunB and imiquimod psoriasis mouse model allowed us to study the therapeutic mechanism of this drug. Top differentially expressed genes in the early response to dithranol belonged to keratinocyte and epidermal differentiation pathways and IL-1 family members (i. Read More

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http://dx.doi.org/10.7554/eLife.56991DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7266641PMC

Controlling the Reproducibility of AC50 Estimation during Compound Profiling through Bayesian β-Expectation Tolerance Intervals.

SLAS Discov 2020 May 29:2472555220918201. Epub 2020 May 29.

Janssen R&D, Translational Medicine and Early Development Statistics, Beerse, Antwerpen, Belgium.

During drug discovery, compounds/biologics are screened against biological targets of interest to find drug candidates with the most desirable activity profile. The compounds are tested at multiple concentrations to understand the dose-response relationship, often summarized as AC50 values and used directly in ranking compounds. Differences between compound repeats are inevitable because of experimental noise and/or systematic error; however, it is often desired to detect the latter when it occurs. Read More

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http://dx.doi.org/10.1177/2472555220918201DOI Listing

Interleukin-29 regulates T follicular helper cells by repressing BCL6 in rheumatoid arthritis patients.

Clin Rheumatol 2020 May 28. Epub 2020 May 28.

Department of Rheumatology and Immunology, China-Japan Union Hospital, Jilin University, No.126 Xiantai Street, Changchun, 130033, Jilin, China.

Introduction: We aimed to investigate whether Interleukin-29 (IL-29) directly affects T follicular helper (Tfh) cell frequency in rheumatoid arthritis (RA), which are both related to RA-specific antibody responses.

Methods: Here, we explored the effect of IL-29 on Tfh cell production in RA patients using a combination of enzyme-linked immunosorbent assay (ELISA), flow cytometry (FCM), CD4 T cell culture, western blotting, and reverse transcription-polymerase chain reaction (RT-PCR).

Results: We reported that serum IL-29 levels, peripheral blood CD4CXCR5 Tfh cell frequency, CD4CXCR5CD40L Tfh cell frequency, and IL-28 receptor (IL-28Rα) and IL-10 receptor (IL-10R2) levels in peripheral blood Tfh cells were higher in RA patients than in healthy controls (HCs). Read More

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http://dx.doi.org/10.1007/s10067-020-05151-yDOI Listing

Oral Biologic Delivery: Advances Towards Oral Subunit, DNA and mRNA Vaccines and the Potential for Mass Vaccination During Pandemics.

Annu Rev Pharmacol Toxicol 2020 May 28. Epub 2020 May 28.

Division of Gastroenterology, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA; email:

Oral vaccination offers the promise of convenient, pain-free and self-administrable vaccine delivery. This is highly attractive in response to pandemic outbreaks where rapid mass vaccination is critical. Furthermore, oral vaccination produces mucosal, as well as systemic, immune responses, which protect against infection at mucosal surfaces. Read More

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http://dx.doi.org/10.1146/annurev-pharmtox-030320-092348DOI Listing