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    Impaired T cell-mediated hepatitis in peroxisome proliferator activated receptor alpha (PPARα)-deficient mice.
    Biol Res 2018 Feb 15;51(1). Epub 2018 Feb 15.
    Department of Nutrition Science, College of Allied Health Sciences, East Carolina University, Health Sciences Bldg. Room 4165F, Greenville, NC, 27834, USA.
    Background: Peroxisome proliferator activated receptor alpha (PPARα), a regulator of enzymes involved in β oxidation, has been reported to influence lymphocyte activation. The purpose of this study was to determine whether PPARα plays a role in T cell-mediated hepatitis induced by Concanavalin A (ConA).

    Methods: Wild type (wt) or PPARα-deficient (PPARα) mice were treated with ConA (15 mg/kg) by intravenous injection 0, 10 or 24 h prior to sacrifice and serum and tissue collection for analysis of tissue injury, cytokine response, T cell activation and characterization. Read More

    Regulation of aquaporins in plants under stress.
    Biol Res 2018 Jan 16;51(1). Epub 2018 Jan 16.
    Department of Renewable Resources, University of Alberta, Edmonton, AB, Canada.
    Aquaporins (AQP) are channel proteins belonging to the Major Intrinsic Protein (MIP) superfamily that play an important role in plant water relations. The main role of aquaporins in plants is transport of water and other small neutral molecules across cellular biological membranes. AQPs have remarkable features to provide an efficient and often, specific water flow and enable them to transport water into and out of the cells along the water potential gradient. Read More

    The tankyrase inhibitor G007-LK inhibits small intestine LGR5stem cell proliferation without altering tissue morphology.
    Biol Res 2018 Jan 9;51(1). Epub 2018 Jan 9.
    Department of Cancer Genetics and SFI CAST, Institute for Cancer Research, Oslo University Hospital, The Norwegian Radium Hospital, 0310, Oslo, Norway.
    Background: The WNT pathway regulates intestinal stem cells and is frequently disrupted in intestinal adenomas. The pathway contains several potential biotargets for interference, including the poly-ADP ribosyltransferase enzymes tankyrase1 and 2. LGR5 is a known WNT pathway target gene and marker of intestinal stem cells. Read More

    Inducible T-cell co-stimulators regulate the proliferation and invasion of human hepatocellular carcinoma HepG2 cells.
    Biol Res 2018 Jan 9;51(1). Epub 2018 Jan 9.
    Departments of Oncology, Affiliated Hospital of Hebei University, Baoding, 071000, China.
    Background: This study determined the regulatory effects of inducible T-cell co-stimulators (ICOS) in human hepatocellular carcinoma HepG2 cells using a RNA interference (RNAi) technique.

    Methods: A RNAi technique was used to knockdown the expression of ICOS. ICOS expression after knockdown was detected as mRNA and protein levels by RT-PCR and Western blot, respectively. Read More

    CCAT2 is an oncogenic long non-coding RNA in pancreatic ductal adenocarcinoma.
    Biol Res 2018 Jan 3;51(1). Epub 2018 Jan 3.
    Department of Geriatric Oncology, The General Hospital of Chinese People's Liberation Army, 28 Fuxing Road, Haidian District, Beijing, 100853, People's Republic of China.
    Background: Pancreatic ductal adenocarcinoma (PDAC) is highly aggressive with poor prognosis. Long non-coding RNAs (lncRNAs), a group of non-coding RNAs, play important roles in the progression of PDAC. This study aimed to investigate the potential involvement of lncRNA CCAT2 in PDAC tumorigenesis. Read More

    Expression regulation and functional analysis of RGS2 and RGS4 in adipogenic and osteogenic differentiation of human mesenchymal stem cells.
    Biol Res 2017 Dec 26;50(1):43. Epub 2017 Dec 26.
    Biological Sciences Department, California State Polytechnic University at Pomona, 3801 W. Temple Ave., Pomona, CA, 91768, USA.
    Background: Understanding the molecular basis underlying the formation of bone-forming osteocytes and lipid-storing adipocytes will help provide insights into the cause of disorders originating in stem/progenitor cells and develop therapeutic treatments for bone- or adipose-related diseases. In this study, the role of RGS2 and RGS4, two members of the regulators of G protein signaling (RGS) family, was investigated during adipogenenic and osteogenenic differentiation of human mesenchymal stem cells (hMSCs).

    Results: Expression of RGS2 and RGS4 were found to be inversely regulated during adipogenesis induced by dexamethasone (DEX) and 3-isobutyl-methylxanthine, regardless if insulin was present, with RGS2 up-regulated and RGS4 down-regulated in response to adipogenic induction. Read More

    Effect of fresh frozen plasma on the in vitro activation of U937 monocytes: a potential role for the age of blood donors and their underlying cytokine profile.
    Biol Res 2017 Dec 21;50(1):42. Epub 2017 Dec 21.
    Department of Immunology, Instituto Nacional de Cardiología Ignacio Chávez, Juan Badiano No1, Col Sección XVI, 14080, Mexico City, Mexico.
    Background: Fresh frozen plasma (FFP) administration may increase the risk of nosocomial infections in parallel with the development of immune modulation. This could be driven by soluble mediators, possibly influencing the in vitro activation of human U937 monocyte cells, in a manner dependent on the age of the donors.

    Methods: FFP donors were stratified into groups of 19-30 years, 31-40 years or 41-50 years, and U937 cells were cultured with FFP (alone or plus lipopolysaccharide-LPS) for 24 h. Read More

    Methanolic extract of Euchelus asper exhibits in-ovo anti-angiogenic and in vitro anti-proliferative activities.
    Biol Res 2017 Dec 12;50(1):41. Epub 2017 Dec 12.
    Central Research Laboratory, D. G. Ruparel College, Senapati Bapat Marg, Mahim, Mumbai, 400 016, India.
    Background: The marine environment is a rich source of bioactive natural products. Many of the marine bioactive compounds have been derived successfully from molluscs. Euchelus asper is a marine mollusc which is commonly found in the intertidal rocky regions of the Mumbai coast. Read More

    Programmed cell death 5 transgenic mice attenuates adjuvant induced arthritis by 2 modifying the T lymphocytes balance.
    Biol Res 2017 Dec 11;50(1):40. Epub 2017 Dec 11.
    Arthritis Clinic & Research Center, Peking University People's Hospital, 11 Xizhimen South Street, Xicheng District, Beijing, 100044, China.
    Background: Programmed cell death 5 (PDCD5) is an apoptosis-related gene cloned from TF-1 cells whose primary biological functions are to promote apoptosis and immune regulation. The effects and mechanisms exerted by key mediators of arthritic inflammation remain unclear in PDCD5 transgenic (PDCD5 tg) mice.

    Results: In the current study, PDCD5 tg mice inhibited the progression of adjuvant-induced arthritis, specifically decreasing clinical signs and histological damage, compared with arthritis control mice. Read More

    Molecular and functional characterization of ferredoxin NADP(H) oxidoreductase from Gracilaria chilensis and its complex with ferredoxin.
    Biol Res 2017 Dec 8;50(1):39. Epub 2017 Dec 8.
    Laboratorio de Biofísica Molecular, Departamento de Bioquímica y Biología Molecular, Facultad de Ciencias Biológicas, Universidad de Concepción, Barrio Universitario S/N, Casilla 160_C, Concepción, Chile.
    Backgroud: Ferredoxin NADP(H) oxidoreductases (EC 1.18.1. Read More

    Synaptic configuration of quadrivalents and their association with the XY bivalent in spermatocytes of Robertsonian heterozygotes of Mus domesticus.
    Biol Res 2017 Nov 23;50(1):38. Epub 2017 Nov 23.
    Departamento de Tecnología Médica, Facultad de Medicina, Universidad de Chile, Santiago, Chile.
    Background: The nuclear architecture of meiotic prophase spermatocytes is based on higher-order patterns of spatial associations among chromosomal domains and consequently is prone to modification by chromosomal rearrangements. We have shown that nuclear architecture is modified in spermatocytes of Robertsonian (Rb) homozygotes of Mus domesticus. In this study we analyse the synaptic configuration of the quadrivalents formed in the meiotic prophase of spermatocytes of mice double heterozygotes for the dependent Rb chromosomes: Rbs 11. Read More

    Role of regenerating gene IA expression on local invasion and survival in nasopharyngeal carcinoma.
    Biol Res 2017 Nov 21;50(1):37. Epub 2017 Nov 21.
    Department of Epidemiology, Public Health College of Xiamen University, Xiamen, 361005, Fujian, China.
    Background: Regenerating gene IA (REGIA) plays an important role in tissue regeneration and tumors prognosis of epithelium origin. However, the role of REGIA in nasopharyngeal carcinoma (NPC) is unclear. This study aims to investigate the expression and function of REG1A in NPC. Read More

    Piceatannol induced apoptosis through up-regulation of microRNA-181a in melanoma cells.
    Biol Res 2017 Oct 17;50(1):36. Epub 2017 Oct 17.
    Department of Dermatology, Chongqing Traditional Chinese Medicine Hospital, Chongqing, 400021, China.
    Background: Melanoma took top position among the lethal cancers and, despite there have been some great attempts made to increase the natural life of patients with metastatic disease, long-lasting and complete remissions are few. Piceatannol, owns the similar function as resveratrol, has been defined as an anti-cancer agent playing important role in inhibition of proliferation, migration and metastasis in various cancer. Thus, we aim to investigate the anti-cancer effect and mechanisms of piceatannol in melanoma cells. Read More

    Mutations in BRCA1, BRCA2 and other breast and ovarian cancer susceptibility genes in Central and South American populations.
    Biol Res 2017 Oct 6;50(1):35. Epub 2017 Oct 6.
    Human Genetics Program, Institute of Biomedical Sciences (ICBM), School of Medicine, University of Chile, Santiago, Chile.
    Breast cancer (BC) is the most common malignancy among women worldwide. A major advance in the understanding of the genetic etiology of BC was the discovery of BRCA1 and BRCA2 (BRCA1/2) genes, which are considered high-penetrance BC genes. In non-carriers of BRCA1/2 mutations, disease susceptibility may be explained of a small number of mutations in BRCA1/2 and a much higher proportion of mutations in ethnicity-specific moderate- and/or low-penetrance genes. Read More

    The comparative mitogenomics and phylogenetics of the two grouse-grasshoppers (Insecta, Orthoptera, Tetrigoidea).
    Biol Res 2017 Oct 5;50(1):34. Epub 2017 Oct 5.
    College of Oceanology and Food Science, Quanzhou Normal University, Quanzhou, 362000, Fujian, China.
    Objective: This study aimed to reveal the mitochondrial genomes (mtgenomes) of Tetrix japonica and Alulatettix yunnanensis, and the phylogenetics of Orthoptera species.

    Methods: The mtgenomes of A. yunnanensis and T. Read More

    Awareness and current knowledge of breast cancer.
    Biol Res 2017 Oct 2;50(1):33. Epub 2017 Oct 2.
    Laboratory of Neuroanatomy & Neuropsychobiology, Department of Pharmacology, RibeirãoPreto Medical School of the University of São Paulo, AV. Bandeirantes, 3900, RibeirãoPreto, 14049-900, São Paulo, Brazil.
    Breast cancer remains a worldwide public health dilemma and is currently the most common tumour in the globe. Awareness of breast cancer, public attentiveness, and advancement in breast imaging has made a positive impact on recognition and screening of breast cancer. Breast cancer is life-threatening disease in females and the leading cause of mortality among women population. Read More

    Upregulation of lncRNA BANCR associated with the lymph node metastasis and poor prognosis in colorectal cancer.
    Biol Res 2017 Oct 2;50(1):32. Epub 2017 Oct 2.
    Department of Gastrointestinal Surgery, Sichuan Academy of Medical Sciences and Sichuan Provincial People's Hospital, 32 Second West Section of the First Ring Road, Chengdu, 610072, Sichuan, China.
    Background: Growing evidence has supported that long non-coding RNAs (lncRNAs) could play vital roles in the development, progression, and prognosis of colorectal cancer (CRC). However, little is known about the clinical significance of BRAF-activated non-coding RNA (BANCR) in CRC. The aim of this study is to explore the clinical value of lncRNA BANCR in CRC patients. Read More

    A simple and rapid identification method for newly emerged porcine Deltacoronavirus with loop-mediated isothermal amplification.
    Biol Res 2017 Sep 21;50(1):30. Epub 2017 Sep 21.
    Department of Veterinary Preventive Medicine, College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang, Jiangxi, China.
    Background: Porcine Deltacoronavirus (PDCoV) is a newly emerged enteropathogenic coronavirus that causes diarrhea and mortality in neonatal piglets. PDCoV has spread to many countries around the world, leading to significant economic losses in the pork industry. Therefore, a rapid and sensitive method for detection of PDCoV in clinical samples is urgently needed. Read More

    MiR-320 inhibits the growth of glioma cells through downregulating PBX3.
    Biol Res 2017 Sep 21;50(1):31. Epub 2017 Sep 21.
    Central Laboratory, Provincial Hospital Affiliated to Shandong University, 544 Jingwu Road, Jinan, 250021, China.
    Background: MiR-320 is downregulated in multiple cancers, including glioma and acts as tumor suppressor through inhibiting tumor cells proliferation and inducing apoptosis. PBX3 (Pre-B cell leukemia homeobox 3), a putative target gene of miR-320, has been reported to be upregulated in various tumors and promote tumor cell growth through regulating MAKP/ERK pathway. This study aimed to verify whether miR-320 influences glioma cells growth through regulating PBX3. Read More

    Neurochemical and behavioral characterization of neuronal glutamate transporter EAAT3 heterozygous mice.
    Biol Res 2017 Sep 19;50(1):29. Epub 2017 Sep 19.
    Laboratorio de Neurogenética, Centro de Neurobiología y Plasticidad Cerebral, Instituto de Fisiología, Facultad de Ciencias, Universidad de Valparaíso, Valparaíso, Chile.
    Background: Obsessive-compulsive disorder (OCD) is a severe neuropsychiatric condition affecting 1-3% of the worldwide population. OCD has a strong genetic component, and the SLC1A1 gene that encodes neuronal glutamate transporter EAAT3 is a strong candidate for this disorder. To evaluate the impact of reduced EAAT3 expression in vivo, we studied male EAAT3 heterozygous and wild-type littermate mice using a battery of behavioral paradigms relevant to anxiety (open field test, elevated plus maze) and compulsivity (marble burying), as well as locomotor activity induced by amphetamine. Read More

    Tridax procumbens flavonoids: a prospective bioactive compound increased osteoblast differentiation and trabecular bone formation.
    Biol Res 2017 Sep 8;50(1):28. Epub 2017 Sep 8.
    Department of Pharmacy, Rajshahi University, Rajshahi, 6205, Bangladesh.
    Background: The Tridax procumbens extracts (TPE) are known for their ethno-medicinal properties to increase osteogenic functioning in mesenchymal stem cells. Recently, we found that the T. procumbens flavonoids (TPF) significantly suppressed the RANKL-induced osteoclasts differentiation and bone resorption. Read More

    miR-22 suppresses tumorigenesis and improves radiosensitivity of breast cancer cells by targeting Sirt1.
    Biol Res 2017 Sep 7;50(1):27. Epub 2017 Sep 7.
    Department of Diagnostic and Interventional Radiology, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, No. 600 Yi Shan Road, Shanghai, 200233, China.
    Background: miR-22 has been shown to be frequently downregulated and act as a tumor suppressor in multiple cancers including breast cancers. However, the role of miR-22 in regulating the radioresistance of breast cancer cells, as well as its underlying mechanism is still not well understood.

    Methods: The expressions of miR-22 and sirt1 at mRNA and protein levels were examined by qRT-PCR and Western Blot. Read More

    Neuron-derived CCL2 contributes to microglia activation and neurological decline in hepatic encephalopathy.
    Biol Res 2017 Sep 4;50(1):26. Epub 2017 Sep 4.
    Department of Radiology, The Second People's Hospital of Lanzhou, No. 388 Jingyuan Road, Chengguan District, Lanzhou, 730046, China.
    Background: CCL2 was up-regulated in neurons and involved in microglia activation and neurological decline in mice suffering from hepatic encephalopathy (HE). However, no data exist concerning the effect of neuron-derived CCL2 on microglia activation in vitro.

    Methods: The rats were pretreated with CCL2 receptor inhibitors (INCB or C021, 1 mg/kg/day i. Read More

    A review on plant importance, biotechnological aspects, and cultivation challenges of jojoba plant.
    Biol Res 2017 Aug 24;50(1):25. Epub 2017 Aug 24.
    Soil Fertility and Microbiology Department, Desert Research Center, Cairo, Egypt.
    Jojoba is considered a promising oil crop and is cultivated for diverse purposes in many countries. The jojoba seed produces unique high-quality oil with a wide range of applications such as medical and industrial-related products. The plant also has potential value in combatting desertification and land degradation in dry and semi-dry areas. Read More

    TMPYP4 exerted antitumor effects in human cervical cancer cells through activation of p38 mitogen-activated protein kinase.
    Biol Res 2017 Jul 3;50(1):24. Epub 2017 Jul 3.
    Department of Gynaecology, Shanghai Jiading District Maternal and Child Care Hospital, No. 1216, Gaotai Road, Jiading District, Shanghai, 201821, China.
    Background: The aim of the present study was to investigate the potential effects of the 5,10,15,20-tetrakis (1-methylpyridinium-4-yl) porphyrin (TMPyP4) on the proliferation and apoptosis of human cervical cancer cells and the underlying mechanisms by which TMPyP4 exerted its actions.

    Results: After human cervical cancer cells were treated with different doses of TMPyP4, cell viability was determined by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) method, the apoptosis was observed by flow cytometry (FCM), and the expression of p38 mitogen-activated protein kinase (MAPK), phosphated p38 MAPK (p-p38 MAPK), capase-3, MAPKAPK2 (MK-2) and poly ADP-ribose polymerase (PARP) was measured by Western blot analysis. The analysis revealed that TMPyP4 potently suppressed cell viability and induced the apoptosis of human cervical cancer cells in a dose-dependent manner. Read More

    S100A8 inhibits PDGF-induced proliferation of airway smooth muscle cells dependent on the receptor for advanced glycation end-products.
    Biol Res 2017 Jun 21;50(1):23. Epub 2017 Jun 21.
    Laboratory of Molecular Biology, Shanghai Research Institute of Acupuncture and Meridian, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, 650 South Wanping Road, Shanghai, 200030, China.
    Background: Airway remodeling is a key feature of asthma, characterized by increased proliferation of airway smooth muscle cells (ASMCs). S100A8 is a calcium-binding protein with a potential to regulate cell proliferation. Here, the effect of exogenous S100A8 protein on the proliferation of ASMCs induced by platelet-derived growth factor (PDGF) and the underlying molecular mechanism was investigated. Read More

    MicroRNA-98 inhibits the cell proliferation of human hypertrophic scar fibroblasts via targeting Col1A1.
    Biol Res 2017 Jun 19;50(1):22. Epub 2017 Jun 19.
    Department of Plastic and Reconstructive Surgery, Southwest Hospital, Third Military Medical University, 29 Gaotanyan Main Street, Shapingba District, Chongqing, 400038, China.
    Background: Hypertrophic scarring (HS) is a severe disease, and results from unusual wound healing. Col1A1 could promote the hypertrophic scar formation, and the expression of Col1A1 in HS tissue was markedly higher than that in the normal. In present study, we aimed to identify miRNAs as post-transcriptional regulators of Col1A1 in HS. Read More

    Rapid culture-based detection of living mycobacteria using microchannel electrical impedance spectroscopy (m-EIS).
    Biol Res 2017 Jun 10;50(1):21. Epub 2017 Jun 10.
    Department of Bioengineering, University of Missouri, 252 Agricultural Engineering Building, 1406 E. Rollins Street, Columbia, MO, 65211, USA.
    Background: Multiple techniques exist for detecting Mycobacteria, each having its own advantages and drawbacks. Among them, automated culture-based systems like the BACTEC-MGIT™ are popular because they are inexpensive, reliable and highly accurate. However, they have a relatively long "time-to-detection" (TTD). Read More

    Meristem culture and subsequent micropropagation of Chilean strawberry (Fragaria chiloensis (L.) Duch.).
    Biol Res 2017 Jun 2;50(1):20. Epub 2017 Jun 2.
    Sociedad de Investigación y Servicios, BioTECNOS Ltda., Talca, Chile.
    Background: Vegetative propagation of Fragaria sp. is traditionally carried out using stolons. This system of propagation, in addition to being slow, can spread plant diseases, particularly serious being viral. Read More

    BRD4 inhibition suppresses cell growth, migration and invasion of salivary adenoid cystic carcinoma.
    Biol Res 2017 May 25;50(1):19. Epub 2017 May 25.
    Department of Periodontology, School of Stomatology, Shandong University, 44-1 West Wen Hua Road, Jinan, 250012, Shandong, People's Republic of China.
    Background: Bromodomain-containing protein 4 (BRD4) inhibition is a new therapeutic strategy for many malignancies. In this study, we aimed to explore the effect of BRD4 inhibition by JQ1 on in vitro cell growth, migration and invasion of salivary adenoid cystic carcinoma (SACC).

    Methods: The human normal epithelial cells and SACC cells (ACC-LM and ACC-83) were treated with JQ1 at concentrations of 0, 0. Read More

    Expression of cocaine- and amphetamine-regulated transcript (CART) in hen ovary.
    Biol Res 2017 May 22;50(1):18. Epub 2017 May 22.
    College of Animal Science and Technology, Shanxi Agricultural University, Taigu, 030801, Shanxi, China.
    Background: Cocaine- and amphetamine-regulated transcript (CART), discovered initially by via differential display RT-PCR analysis of brains of rats administered cocaine, is expressed mainly in central nervous system or neuronal origin cells, and is involved in a wide range of behaviors, such as regulation of food intake, energy homeostasis, and reproduction. The hens egg-laying rate mainly depends on the developmental status of follicles, expression of CART have not been identified from hen follicles, the regulatory mechanisms of CART biological activities are still unknown. The objective of this study was to characterize the mRNA expression of CART in hen follicular granulosa cells and determine CART peptide localization and regulatory role during follicular development. Read More

    Properties of realgar bioleaching using an extremely acidophilic bacterium and its antitumor mechanism as an anticancer agent.
    Biol Res 2017 May 22;50(1):17. Epub 2017 May 22.
    School of Pharmacy, Lanzhou University, Donggang West Road No. 199, Lanzhou, 730020, People's Republic of China.
    Realgar is a naturally occurring arsenic sulfide (or Xionghuang, in Chinese). It contains over 90% tetra-arsenic tetra-sulfide (AsS). Currently, realgar has been confirmed the antitumor activities, both in vitro and in vivo, of realgar extracted using Acidithiobacillus ferrooxidans (A. Read More

    Photosynthesis at the far-red region of the spectrum in Acaryochloris marina.
    Biol Res 2017 May 19;50(1):16. Epub 2017 May 19.
    Department of Chemistry, College of Science, King Saud University, Riyad, Saudi Arabia.
    Acaryochloris marina is an oxygenic cyanobacterium that utilizes far-red light for photosynthesis. It has an expanded genome, which helps in its adaptability to the environment, where it can survive on low energy photons. Its major light absorbing pigment is chlorophyll d and it has α-carotene as a major carotenoid. Read More

    Knockdown of ubiquitin-specific peptidase 39 inhibited the growth of osteosarcoma cells and induced apoptosis in vitro.
    Biol Res 2017 Apr 12;50(1):15. Epub 2017 Apr 12.
    Department of Medical Oncology, The Affiliated 6th People's Hospital of Shanghai Jiaotong University, Shanghai, 200223, China.
    Background: Ubiquitin specific peptidase 39 (USP39), an essential factor in the assembly of the mature spliceosome complex, has an aberrant expression in several cancer. However, its function and the corresponding mechanism on human osteosarcoma has not been fully explored yet.

    Methods: The mRNA and DNA copies of USP39 were increased in osteosarcoma cancer tissues compared with the one in human normal tissues according to datasets from the publicly available Oncomine database. Read More

    Morphological, molecular and FTIR spectroscopic analysis during the differentiation of kidney cells from pluripotent stem cells.
    Biol Res 2017 Apr 4;50(1):14. Epub 2017 Apr 4.
    Centro de Investigación en Biotecnología Aplicada, CIBA-Tlaxcala, Instituto Politécnico Nacional, 90700, Tepetitla, Tlaxcala, Mexico.
    Background: Kidney diseases are a global health problem. Currently, over 2 million people require dialysis or transplant which are associated with high morbidity and mortality; therefore, new researches focused on regenerative medicine have been developed, including the use of stem cells.

    Results: In this research, we generate differentiated kidney cells (DKCs) from mouse pluripotent stem cells (mPSCs) analyzing their morphological, genetic, phenotypic, and spectroscopic characteristics along differentiation, highlighting that there are no reports of the use of Fourier transform infrared (FTIR) spectroscopy to characterize the directed differentiation of mPSCs to DKCs. Read More

    Proton NMR characterization of intact primary and metastatic melanoma cells in 2D & 3D cultures.
    Biol Res 2017 Mar 16;50(1):12. Epub 2017 Mar 16.
    Department of Biomedical Engineering, National University of Singapore, E1-08-016, 9 Engineering Drive 1, Singapore, 117575, Singapore.
    Objective: To characterize the differences between the primary and metastatic melanoma cell lines grown in 2D cultures and 3D cultures.

    Methods: Primary melanoma cells (WM115) and metastatic melanoma cells (WM266) extracted from a single donor was cultured in 2D as well as 3D cultures. These cells were characterized using proton NMR spectrometry, and the qualitative chemical shifts markers were identified and discussed. Read More

    Does Cimicifuga racemosa have the effects like estrogen on the sublingual gland in ovariectomized rats?
    Biol Res 2017 Mar 14;50(1):11. Epub 2017 Mar 14.
    Beijing Shijitan Hospital, Capital Medical University, 10 Tieyi Road, Haidian District, Beijing, China.
    Background: Cimicifuga racemosa is one of the herbs used for the treatment of climacteric syndrome, and it has been cited as an alternative therapy to estrogen. Apart from hectic fevers, dyspareunia and so on, dry mouth also increase significantly after menopause. It has not yet been reported whether C. Read More

    Musk gland seasonal development and musk secretion are regulated by the testis in muskrat (Ondatra zibethicus).
    Biol Res 2017 Mar 4;50(1):10. Epub 2017 Mar 4.
    College of Nature Conservation, Beijing Forestry University, Beijing, 100083, People's Republic of China.
    Background: The muskrat is a seasonal breeder. Males secrete musk to attract females during the breeding season. The testosterone binding to the androgen receptor (AR) in musk glands of muskrat may play an important role conducting the musk secretion process. Read More

    Alpinia oxyphylla Miq. extract changes miRNA expression profiles in db-/db- mouse kidney.
    Biol Res 2017 Mar 1;50(1). Epub 2017 Mar 1.
    Affiliated Hospital of Hainan Medical College, Haikou, 571199, China.
    Background: A number of dysregulated miRNAs have been identified and are proposed to have significant roles in the pathogenesis of type 2 diabetes mellitus or renal pathology. Alpinia oxyphylla has shown significant anti-inflammatory properties and play an anti-diabetes role. The objective of this study was to detect the alteration of miRNAs underlying the anti-diabetes effects of A. Read More

    BJ-1108, a 6-Amino-2,4,5-trimethylpyridin-3-ol analogue, regulates differentiation of Th1 and Th17 cells to ameliorate experimental autoimmune encephalomyelitis.
    Biol Res 2017 Feb 28;50(1). Epub 2017 Feb 28.
    College of Pharmacy, Yeungnam University, Gyeongsan, 38541, Republic of Korea.
    Background: CD4T cells play an important role in the initiation of an immune response by providing help to other cells. Among the helper T subsets, interferon-γ (IFN-γ)-secreting T helper 1 (Th1) and IL-17-secreting T helper 17 (Th17) cells are indispensable for clearance of intracellular as well as extracellular pathogens. However, Th1 and Th17 cells are also associated with pathogenesis and contribute to the progression of multiple inflammatory conditions and autoimmune diseases. Read More

    De novo transcriptome assembly, functional annotation and differential gene expression analysis of juvenile and adult E. fetida, a model oligochaete used in ecotoxicological studies.
    Biol Res 2017 Feb 27;50(1). Epub 2017 Feb 27.
    School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, 200240, China.
    Background: Earthworms are sensitive to toxic chemicals present in the soil and so are useful indicator organisms for soil health. Eisenia fetida are commonly used in ecotoxicological studies; therefore the assembly of a baseline transcriptome is important for subsequent analyses exploring the impact of toxin exposure on genome wide gene expression.

    Results: This paper reports on the de novo transcriptome assembly of E. Read More

    Analysis of gene expression changes associated with human carcinoma-associated fibroblasts in non-small cell lung carcinoma.
    Biol Res 2017 Feb 23;50(1). Epub 2017 Feb 23.
    Department of Oncology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Jiefang Avenue 1095, Wuhan, 430030, China.
    Background: This study aimed to investigate the gene expression changes associated with carcinoma-associated fibroblasts (CAFs) involving in non-small cell lung carcinoma (NSCLC).

    Methods: We downloaded the GEO series GSE22862, which contained matched gene expression values for 15 CAF and normal fibroblasts samples, and series GSE27289 containing SNP genotyping for four matched NSCLC samples. The differentially expressed genes in CAF samples were identified using the limma package in R. Read More

    Differential roles for pathogenicity islands SPI-13 and SPI-8 in the interaction of Salmonella Enteritidis and Salmonella Typhi with murine and human macrophages.
    Biol Res 2017 Feb 15;50(1). Epub 2017 Feb 15.
    Laboratorio de Microbiología, Departamento de Bioquímica y Biología Molecular, Facultad de Ciencias Químicas y Farmacéuticas, Universidad de Chile, Santos Dumont 964, Independencia, Santiago, Chile.
    Background: Salmonella pathogenicity island (SPI)-13 is conserved in many serovars of S. enterica, including S. Enteritidis, S. Read More

    Metabotropic glutamate receptor 5 may be involved in macrophage plasticity.
    Biol Res 2017 Feb 14;50(1). Epub 2017 Feb 14.
    Ilia State University, Tbilisi, Georgia.
    Background: Macrophages are a functionally heterogeneous cell population and depending on microenvironments they polarize in two main groups: M1 and M2. Glutamic acid and glutamate receptors may participate in the regulation of macrophage plasticity. To investigate the role of glutamatergic systems in macrophages physiology, we performed the transfection of mGluR5 cDNAs into RAW-264. Read More

    Selective intra-dinucleotide interactions and periodicities of bases separated by K sites: a new vision and tool for phylogeny analyses.
    Biol Res 2017 Feb 13;50(1). Epub 2017 Feb 13.
    Programa de Genética Humana, Instituto de Ciencias Biomédicas (ICBM), Facultad de Medicina, Universidad de Chile, Independencia 1027, Casilla 70061, Independencia, Chile.
    Direct tests of the random or non-random distribution of nucleotides on genomes have been devised to test the hypothesis of neutral, nearly-neutral or selective evolution. These tests are based on the direct base distribution and are independent of the functional (coding or non-coding) or structural (repeated or unique sequences) properties of the DNA. The first approach described the longitudinal distribution of bases in tandem repeats under the Bose-Einstein statistics. Read More

    Uptake of DNA by cancer cells without a transfection reagent.
    Biol Res 2017 Jan 21;50(1). Epub 2017 Jan 21.
    National Laboratory of Medical Molecular Biology, Department of Biochemistry and Molecular Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Dongdan Santiao, Beijing, 100005, China.
    Background: Cancer cells exhibit elevated levels of glucose uptake and may obtain pre-formed, diet-derived fatty acids from the bloodstream to boost their rapid growth; they may also use nucleic acid from their microenvironment. The study of processing nucleic acid by cancer cells will help improve the understanding of the metabolism of cancer. DNA is commonly packaged into a viral or lipid particle to be transferred into cells; this process is called transfection in laboratory. Read More

    A spleen tyrosine kinase inhibitor attenuates the proliferation and migration of vascular smooth muscle cells.
    Biol Res 2017 Jan 18;50(1). Epub 2017 Jan 18.
    Institute for Bio-Medical Convergence, College of Medicine, Catholic Kwandong University, Gangneung-si, Gangwon-do, South Korea.
    Background: Pathologic vascular smooth muscle cell (VSMC) proliferation and migration after vascular injury promotes the development of occlusive vascular disease. Therefore, an effective chemical agent to suppress aberrant proliferation and migration of VSMCs can be a potential therapeutic modality for occlusive vascular disease such as atherosclerosis and restenosis. To find an anti-proliferative chemical agent for VSMCs, we screened an in-house small molecule library, and the selected small molecule was further validated for its anti-proliferative effect on VSMCs using multiple approaches, such as cell proliferation assays, wound healing assays, transwell migration assays, and ex vivo aortic ring assay. Read More

    Klotho sensitive regulation of dendritic cell functions by vitamin E.
    Biol Res 2016 Nov 24;49(1):45. Epub 2016 Nov 24.
    Institute of Genome Research, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Cau Giay, Hanoi, Vietnam.
    Background: Dendritic cells (DCs) are the most potent professional antigen-presenting cells for naive T cells to link innate and acquired immunity. Klotho, an anti-aging protein, participates in the regulation of Cadependent migration in DCs. Vitamin E (VitE) is an essential antioxidant to protect cells from damage and elicits its inhibitory effects on NF-κB-mediated inflammatory response. Read More

    Olfactomedin-like 2 A and B (OLFML2A and OLFML2B) expression profile in primates (human and baboon).
    Biol Res 2016 Nov 8;49(1):44. Epub 2016 Nov 8.
    Departamento de Genética, Universidad Autónoma de Nuevo León, Hospital Universitario "Dr. José Eleuterio González", 64460, Monterrey, Nuevo León, Mexico.
    Background: The olfactomedin-like domain (OLFML) is present in at least four families of proteins, including OLFML2A and OLFML2B, which are expressed in adult rat retina cells. However, no expression of their orthologous has ever been reported in human and baboon.

    Objective: The aim of this study was to investigate the expression of OLFML2A and OLFML2B in ocular tissues of baboons (Papio hamadryas) and humans, as a key to elucidate OLFML function in eye physiology. Read More

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