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    Arsenic trioxide induces cell cycle arrest and affects Trk receptor expression in human neuroblastoma SK-N-SH cells.
    Biol Res 2018 Jun 13;51(1):18. Epub 2018 Jun 13.
    Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene Regulation, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, 510120, China.
    Background: Arsenic trioxide (AsO), a drug that has been used in China for approximately two thousand years, induces cell death in a variety of cancer cell types, including neuroblastoma (NB). The tyrosine kinase receptor (Trk) family comprises three members, namely TrkA, TrkB and TrkC. Various studies have confirmed that TrkA and TrkC expression is associated with a good prognosis in NB, while TrkB overexpression can lead to tumor cell growth and invasive metastasis. Read More

    Melatonin ameliorates HO-induced oxidative stress through modulation of Erk/Akt/NFkB pathway.
    Biol Res 2018 Jun 11;51(1):17. Epub 2018 Jun 11.
    Fish Endocrinology Research Unit, Department of Zoology, University of Calcutta, 35 Ballygunge Circular Road, Kolkata, West Bengal, 700019, India.
    Background: Improper control on reactive oxygen species (ROS) elimination process and formation of free radicals causes tissue dysfunction. Pineal hormone melatonin is considered a potent regulator of such oxidative damage in different vertebrates. Aim of the current communication is to evaluate the levels of oxidative stress and ROS induced damage, and amelioration of oxidative status through melatonin induced activation of signaling pathways. Read More

    The effect of downregulation of Stathmin gene on biological behaviors of U373 and U87-MG glioblastoma cells.
    Biol Res 2018 Jun 8;51(1):16. Epub 2018 Jun 8.
    Department of Oncology, Peace Hospital of Changzhi Medical College, Changzhi, 046000, Shanxi, China.
    Background: Stathmin as a critical protein involved in microtubule polymerization, is necessary for survival of cancer cells. However, extremely little is known about Stathmin in glioblastoma. So, this study was designed to elucidate the function of Stathmin gene in the tumorigenesis and progression of glioblastoma cells. Read More

    Extracellular matrix and fibroblast injection produces pterygium-like lesion in rabbits.
    Biol Res 2018 Jun 5;51(1):15. Epub 2018 Jun 5.
    Tecnologico de Monterrey, Escuela de Medicina y Ciencias de la Salud, Ave. Morones Prieto 3000, 64710, Monterrey, NL, Mexico.
    Background: Translational research to develop pharmaceutical and surgical treatments for pterygium requires a reliable and easy to produce animal model. Extracellular matrix and fibroblast are important components of pterygium. The aim of this study was to analyze the effect of the subconjunctival injection of fibroblast cells (NIH3T3 cell line) and exogenous extracellular matrix in rabbits in producing a pterygium-like lesion. Read More

    The NK1 receptor antagonist NKP608 inhibits proliferation of human colorectal cancer cells via Wnt signaling pathway.
    Biol Res 2018 May 30;51(1):14. Epub 2018 May 30.
    Anorectal Department, Dongzhimen Hospital, Beijing University of Chinese Medicine, Hai Yun Cang on the 5th Zip, Dongcheng District, Beijing, 100700, People's Republic of China.
    Background: Neurokinin1 (NK1) receptor has played a vital role in the development of tumor. However, NKP608 as a NK1 receptor antagonist whether has the effect of the resistance of colorectal cancer is still unclear. Thereby, in this study, we investigated the role of NKP608 on human colorectal cancer and explored the underlying mechanism. Read More

    MicroRNA-1247 inhibits cell proliferation by directly targeting ZNF346 in childhood neuroblastoma.
    Biol Res 2018 May 24;51(1):13. Epub 2018 May 24.
    Department of Neonatology, The First People's Hospital of Jingzhou, No. 8 Hangkong Road, Shashi District, Jingzhou, 434000, Hubei, China.
    Background: Neuroblastoma (NB) represents the most common extracranial solid tumor in children. Accumulating evidence shows that microRNAs (miRs) play an important role in the carcinogenesis of NB. Here, we investigated the biological function of miR-1247 in NB in vitro. Read More

    In vitro chemokine (C-C motif) receptor 6-dependent non-inflammatory chemotaxis during spermatogenesis.
    Biol Res 2018 May 22;51(1):12. Epub 2018 May 22.
    Reproductive Medicine Centre, Shenzhen No. 2 Hospital, Shenzhen University School of Medicine, No. 3002, Sunguang West Road, Shenzhen, 518035, Guangdong, China.
    Background: Chemokine (C-C motif) receptor 6 (CCR6) is present in sperm and plays a significant role in sperm motility and chemotaxis acting in the reproductive tracts. However, the expression and functional significance of CCR6 in testis are still poorly understood, especially in the process of spermatogenesis.

    Methods And Results: CCR6 was expressed in spermatogenic cell lines and its expression was shown in an age-dependent upregulation manner from puberty to adulthood in mouse testis. Read More

    CMPD1 inhibited human gastric cancer cell proliferation by inducing apoptosis and G2/M cell cycle arrest.
    Biol Res 2018 Apr 16;51(1):11. Epub 2018 Apr 16.
    Department of Medical Oncology, The First Affiliated Hospital of Bengbu Medical College, 287 Changhuai Road, Bengbu, 233004, Anhui, People's Republic of China.
    Background: Gastric cancer occupies the fourth highest morbidity rate of cancers worldwide. Clinical therapies of gastric cancer remain limited because of uncertainty of mechanisms and shortness of effective medicine. Thus, new drug candidates for gastric cancer treatment is urgently needed. Read More

    Independent prognostic genes and mechanism investigation for colon cancer.
    Biol Res 2018 Apr 13;51(1):10. Epub 2018 Apr 13.
    Gastrointestinal Colorectal and Anal surgery, China-Japan Union Hospital of Jilin University, No. 126 Xiantai Street, Changchun, Jilin, 130033, China.
    Propose: We aimed to explore the potential molecular mechanism and independent prognostic genes for colon cancer (CC).

    Methods: Microarray datasets GSE17536 and GSE39582 were downloaded from Gene Expression Omnibus. Meanwhile, the whole CC-related dataset were downloaded from The Cancer Genome Atlas (TCGA) database. Read More

    Berberine ameliorates diabetic nephropathy by inhibiting TLR4/NF-κB pathway.
    Biol Res 2018 Mar 31;51(1). Epub 2018 Mar 31.
    Department of Endocrinology, Huaihe Hospital of Henan University, No.115 Ximen Street, Gulou District, Kaifeng, 475000, China.
    Background: Diabetic nephropathy (DN) is the leading cause of end-stage renal failure, contributing to severe morbidity and mortality in diabetic patients. Berberine (BBR) has been well characterized to exert renoprotective effects in DN progression. However, the action mechanism of BBR in DN remains to be fully understood. Read More

    HSP70 from the Antarctic sea urchin Sterechinus neumayeri: molecular characterization and expression in response to heat stress.
    Biol Res 2018 Mar 27;51(1). Epub 2018 Mar 27.
    Laboratorio de Biorrecursos Antárticos, Departamento Científico, Instituto Antártico Chileno, Plaza Muñoz Gamero 1055, Punta Arenas, Chile.
    Background: Heat stress proteins are implicated in stabilizing and refolding denatured proteins in vertebrates and invertebrates. Members of the Hsp70 gene family comprise the cognate heat shock protein (Hsc70) and inducible heat shock protein (Hsp70). However, the cDNA sequence and the expression of Hsp70 in the Antarctic sea urchin are unknown. Read More

    Transcriptome analysis of phosphorus stress responsiveness in the seedlings of Dongxiang wild rice (Oryza rufipogon Griff.).
    Biol Res 2018 Mar 15;51(1). Epub 2018 Mar 15.
    College of Life Science, Jiangxi Normal University, Nanchang, 330022, China.
    Background: Low phosphorus availability is a major factor restricting rice growth. Dongxiang wild rice (Oryza rufipogon Griff.) has many useful genes lacking in cultivated rice, including stress resistance to phosphorus deficiency, cold, salt and drought, which is considered to be a precious germplasm resource for rice breeding. Read More

    Study on the relationship between expression patterns of cocaine-and amphetamine regulated transcript and hormones secretion in porcine ovarian follicles.
    Biol Res 2018 Feb 26;51(1). Epub 2018 Feb 26.
    College of Animal Science and Technology, Shanxi Agricultural University, Taigu, 030801, Shanxi, China.
    Background: Cocaine-and amphetamine regulated transcript (CART) is an endogenous neuropeptide, which is widespread in animals, plays a key role in regulation of follicular atresia in cattle and sheep. Among animal ovaries, CART mRNA was firstly found in the cattle ovaries. CART was localized in the antral follicles oocytes, granulosa and cumulus cells by immunohistochemistry and in situ hybridization. Read More

    Impaired T cell-mediated hepatitis in peroxisome proliferator activated receptor alpha (PPARα)-deficient mice.
    Biol Res 2018 Feb 15;51(1). Epub 2018 Feb 15.
    Department of Nutrition Science, College of Allied Health Sciences, East Carolina University, Health Sciences Bldg. Room 4165F, Greenville, NC, 27834, USA.
    Background: Peroxisome proliferator activated receptor alpha (PPARα), a regulator of enzymes involved in β oxidation, has been reported to influence lymphocyte activation. The purpose of this study was to determine whether PPARα plays a role in T cell-mediated hepatitis induced by Concanavalin A (ConA).

    Methods: Wild type (wt) or PPARα-deficient (PPARα) mice were treated with ConA (15 mg/kg) by intravenous injection 0, 10 or 24 h prior to sacrifice and serum and tissue collection for analysis of tissue injury, cytokine response, T cell activation and characterization. Read More

    Regulation of aquaporins in plants under stress.
    Biol Res 2018 Jan 16;51(1). Epub 2018 Jan 16.
    Department of Renewable Resources, University of Alberta, Edmonton, AB, Canada.
    Aquaporins (AQP) are channel proteins belonging to the Major Intrinsic Protein (MIP) superfamily that play an important role in plant water relations. The main role of aquaporins in plants is transport of water and other small neutral molecules across cellular biological membranes. AQPs have remarkable features to provide an efficient and often, specific water flow and enable them to transport water into and out of the cells along the water potential gradient. Read More

    The tankyrase inhibitor G007-LK inhibits small intestine LGR5 stem cell proliferation without altering tissue morphology.
    Biol Res 2018 Jan 9;51(1). Epub 2018 Jan 9.
    Department of Cancer Genetics and SFI CAST, Institute for Cancer Research, Oslo University Hospital, The Norwegian Radium Hospital, 0310, Oslo, Norway.
    Background: The WNT pathway regulates intestinal stem cells and is frequently disrupted in intestinal adenomas. The pathway contains several potential biotargets for interference, including the poly-ADP ribosyltransferase enzymes tankyrase1 and 2. LGR5 is a known WNT pathway target gene and marker of intestinal stem cells. Read More

    Inducible T-cell co-stimulators regulate the proliferation and invasion of human hepatocellular carcinoma HepG2 cells.
    Biol Res 2018 Jan 9;51(1). Epub 2018 Jan 9.
    Departments of Oncology, Affiliated Hospital of Hebei University, Baoding, 071000, China.
    Background: This study determined the regulatory effects of inducible T-cell co-stimulators (ICOS) in human hepatocellular carcinoma HepG2 cells using a RNA interference (RNAi) technique.

    Methods: A RNAi technique was used to knockdown the expression of ICOS. ICOS expression after knockdown was detected as mRNA and protein levels by RT-PCR and Western blot, respectively. Read More

    CCAT2 is an oncogenic long non-coding RNA in pancreatic ductal adenocarcinoma.
    Biol Res 2018 Jan 3;51(1). Epub 2018 Jan 3.
    Department of Geriatric Oncology, The General Hospital of Chinese People's Liberation Army, 28 Fuxing Road, Haidian District, Beijing, 100853, People's Republic of China.
    Background: Pancreatic ductal adenocarcinoma (PDAC) is highly aggressive with poor prognosis. Long non-coding RNAs (lncRNAs), a group of non-coding RNAs, play important roles in the progression of PDAC. This study aimed to investigate the potential involvement of lncRNA CCAT2 in PDAC tumorigenesis. Read More

    Expression regulation and functional analysis of RGS2 and RGS4 in adipogenic and osteogenic differentiation of human mesenchymal stem cells.
    Biol Res 2017 Dec 26;50(1):43. Epub 2017 Dec 26.
    Biological Sciences Department, California State Polytechnic University at Pomona, 3801 W. Temple Ave., Pomona, CA, 91768, USA.
    Background: Understanding the molecular basis underlying the formation of bone-forming osteocytes and lipid-storing adipocytes will help provide insights into the cause of disorders originating in stem/progenitor cells and develop therapeutic treatments for bone- or adipose-related diseases. In this study, the role of RGS2 and RGS4, two members of the regulators of G protein signaling (RGS) family, was investigated during adipogenenic and osteogenenic differentiation of human mesenchymal stem cells (hMSCs).

    Results: Expression of RGS2 and RGS4 were found to be inversely regulated during adipogenesis induced by dexamethasone (DEX) and 3-isobutyl-methylxanthine, regardless if insulin was present, with RGS2 up-regulated and RGS4 down-regulated in response to adipogenic induction. Read More

    Effect of fresh frozen plasma on the in vitro activation of U937 monocytes: a potential role for the age of blood donors and their underlying cytokine profile.
    Biol Res 2017 Dec 21;50(1):42. Epub 2017 Dec 21.
    Department of Immunology, Instituto Nacional de Cardiología Ignacio Chávez, Juan Badiano No1, Col Sección XVI, 14080, Mexico City, Mexico.
    Background: Fresh frozen plasma (FFP) administration may increase the risk of nosocomial infections in parallel with the development of immune modulation. This could be driven by soluble mediators, possibly influencing the in vitro activation of human U937 monocyte cells, in a manner dependent on the age of the donors.

    Methods: FFP donors were stratified into groups of 19-30 years, 31-40 years or 41-50 years, and U937 cells were cultured with FFP (alone or plus lipopolysaccharide-LPS) for 24 h. Read More

    Methanolic extract of Euchelus asper exhibits in-ovo anti-angiogenic and in vitro anti-proliferative activities.
    Biol Res 2017 Dec 12;50(1):41. Epub 2017 Dec 12.
    Central Research Laboratory, D. G. Ruparel College, Senapati Bapat Marg, Mahim, Mumbai, 400 016, India.
    Background: The marine environment is a rich source of bioactive natural products. Many of the marine bioactive compounds have been derived successfully from molluscs. Euchelus asper is a marine mollusc which is commonly found in the intertidal rocky regions of the Mumbai coast. Read More

    Programmed cell death 5 transgenic mice attenuates adjuvant induced arthritis by 2 modifying the T lymphocytes balance.
    Biol Res 2017 Dec 11;50(1):40. Epub 2017 Dec 11.
    Arthritis Clinic & Research Center, Peking University People's Hospital, 11 Xizhimen South Street, Xicheng District, Beijing, 100044, China.
    Background: Programmed cell death 5 (PDCD5) is an apoptosis-related gene cloned from TF-1 cells whose primary biological functions are to promote apoptosis and immune regulation. The effects and mechanisms exerted by key mediators of arthritic inflammation remain unclear in PDCD5 transgenic (PDCD5 tg) mice.

    Results: In the current study, PDCD5 tg mice inhibited the progression of adjuvant-induced arthritis, specifically decreasing clinical signs and histological damage, compared with arthritis control mice. Read More

    Molecular and functional characterization of ferredoxin NADP(H) oxidoreductase from Gracilaria chilensis and its complex with ferredoxin.
    Biol Res 2017 Dec 8;50(1):39. Epub 2017 Dec 8.
    Laboratorio de Biofísica Molecular, Departamento de Bioquímica y Biología Molecular, Facultad de Ciencias Biológicas, Universidad de Concepción, Barrio Universitario S/N, Casilla 160_C, Concepción, Chile.
    Backgroud: Ferredoxin NADP(H) oxidoreductases (EC 1.18.1. Read More

    Synaptic configuration of quadrivalents and their association with the XY bivalent in spermatocytes of Robertsonian heterozygotes of Mus domesticus.
    Biol Res 2017 Nov 23;50(1):38. Epub 2017 Nov 23.
    Departamento de Tecnología Médica, Facultad de Medicina, Universidad de Chile, Santiago, Chile.
    Background: The nuclear architecture of meiotic prophase spermatocytes is based on higher-order patterns of spatial associations among chromosomal domains and consequently is prone to modification by chromosomal rearrangements. We have shown that nuclear architecture is modified in spermatocytes of Robertsonian (Rb) homozygotes of Mus domesticus. In this study we analyse the synaptic configuration of the quadrivalents formed in the meiotic prophase of spermatocytes of mice double heterozygotes for the dependent Rb chromosomes: Rbs 11. Read More

    Role of regenerating gene IA expression on local invasion and survival in nasopharyngeal carcinoma.
    Biol Res 2017 Nov 21;50(1):37. Epub 2017 Nov 21.
    Department of Epidemiology, Public Health College of Xiamen University, Xiamen, 361005, Fujian, China.
    Background: Regenerating gene IA (REGIA) plays an important role in tissue regeneration and tumors prognosis of epithelium origin. However, the role of REGIA in nasopharyngeal carcinoma (NPC) is unclear. This study aims to investigate the expression and function of REG1A in NPC. Read More

    Piceatannol induced apoptosis through up-regulation of microRNA-181a in melanoma cells.
    Biol Res 2017 Oct 17;50(1):36. Epub 2017 Oct 17.
    Department of Dermatology, Chongqing Traditional Chinese Medicine Hospital, Chongqing, 400021, China.
    Background: Melanoma took top position among the lethal cancers and, despite there have been some great attempts made to increase the natural life of patients with metastatic disease, long-lasting and complete remissions are few. Piceatannol, owns the similar function as resveratrol, has been defined as an anti-cancer agent playing important role in inhibition of proliferation, migration and metastasis in various cancer. Thus, we aim to investigate the anti-cancer effect and mechanisms of piceatannol in melanoma cells. Read More

    Mutations in BRCA1, BRCA2 and other breast and ovarian cancer susceptibility genes in Central and South American populations.
    Biol Res 2017 Oct 6;50(1):35. Epub 2017 Oct 6.
    Human Genetics Program, Institute of Biomedical Sciences (ICBM), School of Medicine, University of Chile, Santiago, Chile.
    Breast cancer (BC) is the most common malignancy among women worldwide. A major advance in the understanding of the genetic etiology of BC was the discovery of BRCA1 and BRCA2 (BRCA1/2) genes, which are considered high-penetrance BC genes. In non-carriers of BRCA1/2 mutations, disease susceptibility may be explained of a small number of mutations in BRCA1/2 and a much higher proportion of mutations in ethnicity-specific moderate- and/or low-penetrance genes. Read More

    The comparative mitogenomics and phylogenetics of the two grouse-grasshoppers (Insecta, Orthoptera, Tetrigoidea).
    Biol Res 2017 Oct 5;50(1):34. Epub 2017 Oct 5.
    College of Oceanology and Food Science, Quanzhou Normal University, Quanzhou, 362000, Fujian, China.
    Objective: This study aimed to reveal the mitochondrial genomes (mtgenomes) of Tetrix japonica and Alulatettix yunnanensis, and the phylogenetics of Orthoptera species.

    Methods: The mtgenomes of A. yunnanensis and T. Read More

    Awareness and current knowledge of breast cancer.
    Biol Res 2017 Oct 2;50(1):33. Epub 2017 Oct 2.
    Laboratory of Neuroanatomy & Neuropsychobiology, Department of Pharmacology, RibeirãoPreto Medical School of the University of São Paulo, AV. Bandeirantes, 3900, RibeirãoPreto, 14049-900, São Paulo, Brazil.
    Breast cancer remains a worldwide public health dilemma and is currently the most common tumour in the globe. Awareness of breast cancer, public attentiveness, and advancement in breast imaging has made a positive impact on recognition and screening of breast cancer. Breast cancer is life-threatening disease in females and the leading cause of mortality among women population. Read More

    Upregulation of lncRNA BANCR associated with the lymph node metastasis and poor prognosis in colorectal cancer.
    Biol Res 2017 Oct 2;50(1):32. Epub 2017 Oct 2.
    Department of Gastrointestinal Surgery, Sichuan Academy of Medical Sciences and Sichuan Provincial People's Hospital, 32 Second West Section of the First Ring Road, Chengdu, 610072, Sichuan, China.
    Background: Growing evidence has supported that long non-coding RNAs (lncRNAs) could play vital roles in the development, progression, and prognosis of colorectal cancer (CRC). However, little is known about the clinical significance of BRAF-activated non-coding RNA (BANCR) in CRC. The aim of this study is to explore the clinical value of lncRNA BANCR in CRC patients. Read More

    A simple and rapid identification method for newly emerged porcine Deltacoronavirus with loop-mediated isothermal amplification.
    Biol Res 2017 Sep 21;50(1):30. Epub 2017 Sep 21.
    Department of Veterinary Preventive Medicine, College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang, Jiangxi, China.
    Background: Porcine Deltacoronavirus (PDCoV) is a newly emerged enteropathogenic coronavirus that causes diarrhea and mortality in neonatal piglets. PDCoV has spread to many countries around the world, leading to significant economic losses in the pork industry. Therefore, a rapid and sensitive method for detection of PDCoV in clinical samples is urgently needed. Read More

    MiR-320 inhibits the growth of glioma cells through downregulating PBX3.
    Biol Res 2017 Sep 21;50(1):31. Epub 2017 Sep 21.
    Central Laboratory, Provincial Hospital Affiliated to Shandong University, 544 Jingwu Road, Jinan, 250021, China.
    Background: MiR-320 is downregulated in multiple cancers, including glioma and acts as tumor suppressor through inhibiting tumor cells proliferation and inducing apoptosis. PBX3 (Pre-B cell leukemia homeobox 3), a putative target gene of miR-320, has been reported to be upregulated in various tumors and promote tumor cell growth through regulating MAKP/ERK pathway. This study aimed to verify whether miR-320 influences glioma cells growth through regulating PBX3. Read More

    Neurochemical and behavioral characterization of neuronal glutamate transporter EAAT3 heterozygous mice.
    Biol Res 2017 Sep 19;50(1):29. Epub 2017 Sep 19.
    Laboratorio de Neurogenética, Centro de Neurobiología y Plasticidad Cerebral, Instituto de Fisiología, Facultad de Ciencias, Universidad de Valparaíso, Valparaíso, Chile.
    Background: Obsessive-compulsive disorder (OCD) is a severe neuropsychiatric condition affecting 1-3% of the worldwide population. OCD has a strong genetic component, and the SLC1A1 gene that encodes neuronal glutamate transporter EAAT3 is a strong candidate for this disorder. To evaluate the impact of reduced EAAT3 expression in vivo, we studied male EAAT3 heterozygous and wild-type littermate mice using a battery of behavioral paradigms relevant to anxiety (open field test, elevated plus maze) and compulsivity (marble burying), as well as locomotor activity induced by amphetamine. Read More

    Tridax procumbens flavonoids: a prospective bioactive compound increased osteoblast differentiation and trabecular bone formation.
    Biol Res 2017 Sep 8;50(1):28. Epub 2017 Sep 8.
    Department of Pharmacy, Rajshahi University, Rajshahi, 6205, Bangladesh.
    Background: The Tridax procumbens extracts (TPE) are known for their ethno-medicinal properties to increase osteogenic functioning in mesenchymal stem cells. Recently, we found that the T. procumbens flavonoids (TPF) significantly suppressed the RANKL-induced osteoclasts differentiation and bone resorption. Read More

    miR-22 suppresses tumorigenesis and improves radiosensitivity of breast cancer cells by targeting Sirt1.
    Biol Res 2017 Sep 7;50(1):27. Epub 2017 Sep 7.
    Department of Diagnostic and Interventional Radiology, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, No. 600 Yi Shan Road, Shanghai, 200233, China.
    Background: miR-22 has been shown to be frequently downregulated and act as a tumor suppressor in multiple cancers including breast cancers. However, the role of miR-22 in regulating the radioresistance of breast cancer cells, as well as its underlying mechanism is still not well understood.

    Methods: The expressions of miR-22 and sirt1 at mRNA and protein levels were examined by qRT-PCR and Western Blot. Read More

    Neuron-derived CCL2 contributes to microglia activation and neurological decline in hepatic encephalopathy.
    Biol Res 2017 Sep 4;50(1):26. Epub 2017 Sep 4.
    Department of Radiology, The Second People's Hospital of Lanzhou, No. 388 Jingyuan Road, Chengguan District, Lanzhou, 730046, China.
    Background: CCL2 was up-regulated in neurons and involved in microglia activation and neurological decline in mice suffering from hepatic encephalopathy (HE). However, no data exist concerning the effect of neuron-derived CCL2 on microglia activation in vitro.

    Methods: The rats were pretreated with CCL2 receptor inhibitors (INCB or C021, 1 mg/kg/day i. Read More

    A review on plant importance, biotechnological aspects, and cultivation challenges of jojoba plant.
    Biol Res 2017 Aug 24;50(1):25. Epub 2017 Aug 24.
    Soil Fertility and Microbiology Department, Desert Research Center, Cairo, Egypt.
    Jojoba is considered a promising oil crop and is cultivated for diverse purposes in many countries. The jojoba seed produces unique high-quality oil with a wide range of applications such as medical and industrial-related products. The plant also has potential value in combatting desertification and land degradation in dry and semi-dry areas. Read More

    TMPYP4 exerted antitumor effects in human cervical cancer cells through activation of p38 mitogen-activated protein kinase.
    Biol Res 2017 Jul 3;50(1):24. Epub 2017 Jul 3.
    Department of Gynaecology, Shanghai Jiading District Maternal and Child Care Hospital, No. 1216, Gaotai Road, Jiading District, Shanghai, 201821, China.
    Background: The aim of the present study was to investigate the potential effects of the 5,10,15,20-tetrakis (1-methylpyridinium-4-yl) porphyrin (TMPyP4) on the proliferation and apoptosis of human cervical cancer cells and the underlying mechanisms by which TMPyP4 exerted its actions.

    Results: After human cervical cancer cells were treated with different doses of TMPyP4, cell viability was determined by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) method, the apoptosis was observed by flow cytometry (FCM), and the expression of p38 mitogen-activated protein kinase (MAPK), phosphated p38 MAPK (p-p38 MAPK), capase-3, MAPKAPK2 (MK-2) and poly ADP-ribose polymerase (PARP) was measured by Western blot analysis. The analysis revealed that TMPyP4 potently suppressed cell viability and induced the apoptosis of human cervical cancer cells in a dose-dependent manner. Read More

    S100A8 inhibits PDGF-induced proliferation of airway smooth muscle cells dependent on the receptor for advanced glycation end-products.
    Biol Res 2017 Jun 21;50(1):23. Epub 2017 Jun 21.
    Laboratory of Molecular Biology, Shanghai Research Institute of Acupuncture and Meridian, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, 650 South Wanping Road, Shanghai, 200030, China.
    Background: Airway remodeling is a key feature of asthma, characterized by increased proliferation of airway smooth muscle cells (ASMCs). S100A8 is a calcium-binding protein with a potential to regulate cell proliferation. Here, the effect of exogenous S100A8 protein on the proliferation of ASMCs induced by platelet-derived growth factor (PDGF) and the underlying molecular mechanism was investigated. Read More

    MicroRNA-98 inhibits the cell proliferation of human hypertrophic scar fibroblasts via targeting Col1A1.
    Biol Res 2017 Jun 19;50(1):22. Epub 2017 Jun 19.
    Department of Plastic and Reconstructive Surgery, Southwest Hospital, Third Military Medical University, 29 Gaotanyan Main Street, Shapingba District, Chongqing, 400038, China.
    Background: Hypertrophic scarring (HS) is a severe disease, and results from unusual wound healing. Col1A1 could promote the hypertrophic scar formation, and the expression of Col1A1 in HS tissue was markedly higher than that in the normal. In present study, we aimed to identify miRNAs as post-transcriptional regulators of Col1A1 in HS. Read More

    Rapid culture-based detection of living mycobacteria using microchannel electrical impedance spectroscopy (m-EIS).
    Biol Res 2017 Jun 10;50(1):21. Epub 2017 Jun 10.
    Department of Bioengineering, University of Missouri, 252 Agricultural Engineering Building, 1406 E. Rollins Street, Columbia, MO, 65211, USA.
    Background: Multiple techniques exist for detecting Mycobacteria, each having its own advantages and drawbacks. Among them, automated culture-based systems like the BACTEC-MGIT™ are popular because they are inexpensive, reliable and highly accurate. However, they have a relatively long "time-to-detection" (TTD). Read More

    Meristem culture and subsequent micropropagation of Chilean strawberry (Fragaria chiloensis (L.) Duch.).
    Biol Res 2017 Jun 2;50(1):20. Epub 2017 Jun 2.
    Sociedad de Investigación y Servicios, BioTECNOS Ltda., Talca, Chile.
    Background: Vegetative propagation of Fragaria sp. is traditionally carried out using stolons. This system of propagation, in addition to being slow, can spread plant diseases, particularly serious being viral. Read More

    BRD4 inhibition suppresses cell growth, migration and invasion of salivary adenoid cystic carcinoma.
    Biol Res 2017 May 25;50(1):19. Epub 2017 May 25.
    Department of Periodontology, School of Stomatology, Shandong University, 44-1 West Wen Hua Road, Jinan, 250012, Shandong, People's Republic of China.
    Background: Bromodomain-containing protein 4 (BRD4) inhibition is a new therapeutic strategy for many malignancies. In this study, we aimed to explore the effect of BRD4 inhibition by JQ1 on in vitro cell growth, migration and invasion of salivary adenoid cystic carcinoma (SACC).

    Methods: The human normal epithelial cells and SACC cells (ACC-LM and ACC-83) were treated with JQ1 at concentrations of 0, 0. Read More

    Expression of cocaine- and amphetamine-regulated transcript (CART) in hen ovary.
    Biol Res 2017 May 22;50(1):18. Epub 2017 May 22.
    College of Animal Science and Technology, Shanxi Agricultural University, Taigu, 030801, Shanxi, China.
    Background: Cocaine- and amphetamine-regulated transcript (CART), discovered initially by via differential display RT-PCR analysis of brains of rats administered cocaine, is expressed mainly in central nervous system or neuronal origin cells, and is involved in a wide range of behaviors, such as regulation of food intake, energy homeostasis, and reproduction. The hens egg-laying rate mainly depends on the developmental status of follicles, expression of CART have not been identified from hen follicles, the regulatory mechanisms of CART biological activities are still unknown. The objective of this study was to characterize the mRNA expression of CART in hen follicular granulosa cells and determine CART peptide localization and regulatory role during follicular development. Read More

    Properties of realgar bioleaching using an extremely acidophilic bacterium and its antitumor mechanism as an anticancer agent.
    Biol Res 2017 May 22;50(1):17. Epub 2017 May 22.
    School of Pharmacy, Lanzhou University, Donggang West Road No. 199, Lanzhou, 730020, People's Republic of China.
    Realgar is a naturally occurring arsenic sulfide (or Xionghuang, in Chinese). It contains over 90% tetra-arsenic tetra-sulfide (AsS). Currently, realgar has been confirmed the antitumor activities, both in vitro and in vivo, of realgar extracted using Acidithiobacillus ferrooxidans (A. Read More

    Photosynthesis at the far-red region of the spectrum in Acaryochloris marina.
    Biol Res 2017 May 19;50(1):16. Epub 2017 May 19.
    Department of Chemistry, College of Science, King Saud University, Riyad, Saudi Arabia.
    Acaryochloris marina is an oxygenic cyanobacterium that utilizes far-red light for photosynthesis. It has an expanded genome, which helps in its adaptability to the environment, where it can survive on low energy photons. Its major light absorbing pigment is chlorophyll d and it has α-carotene as a major carotenoid. Read More

    Knockdown of ubiquitin-specific peptidase 39 inhibited the growth of osteosarcoma cells and induced apoptosis in vitro.
    Biol Res 2017 Apr 12;50(1):15. Epub 2017 Apr 12.
    Department of Medical Oncology, The Affiliated 6th People's Hospital of Shanghai Jiaotong University, Shanghai, 200223, China.
    Background: Ubiquitin specific peptidase 39 (USP39), an essential factor in the assembly of the mature spliceosome complex, has an aberrant expression in several cancer. However, its function and the corresponding mechanism on human osteosarcoma has not been fully explored yet.

    Methods: The mRNA and DNA copies of USP39 were increased in osteosarcoma cancer tissues compared with the one in human normal tissues according to datasets from the publicly available Oncomine database. Read More

    Morphological, molecular and FTIR spectroscopic analysis during the differentiation of kidney cells from pluripotent stem cells.
    Biol Res 2017 Apr 4;50(1):14. Epub 2017 Apr 4.
    Centro de Investigación en Biotecnología Aplicada, CIBA-Tlaxcala, Instituto Politécnico Nacional, 90700, Tepetitla, Tlaxcala, Mexico.
    Background: Kidney diseases are a global health problem. Currently, over 2 million people require dialysis or transplant which are associated with high morbidity and mortality; therefore, new researches focused on regenerative medicine have been developed, including the use of stem cells.

    Results: In this research, we generate differentiated kidney cells (DKCs) from mouse pluripotent stem cells (mPSCs) analyzing their morphological, genetic, phenotypic, and spectroscopic characteristics along differentiation, highlighting that there are no reports of the use of Fourier transform infrared (FTIR) spectroscopy to characterize the directed differentiation of mPSCs to DKCs. Read More

    Proton NMR characterization of intact primary and metastatic melanoma cells in 2D & 3D cultures.
    Biol Res 2017 Mar 16;50(1):12. Epub 2017 Mar 16.
    Department of Biomedical Engineering, National University of Singapore, E1-08-016, 9 Engineering Drive 1, Singapore, 117575, Singapore.
    Objective: To characterize the differences between the primary and metastatic melanoma cell lines grown in 2D cultures and 3D cultures.

    Methods: Primary melanoma cells (WM115) and metastatic melanoma cells (WM266) extracted from a single donor was cultured in 2D as well as 3D cultures. These cells were characterized using proton NMR spectrometry, and the qualitative chemical shifts markers were identified and discussed. Read More

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