3,782 results match your criteria Biological chemistry[Journal]


In memoriam Kanti D. Bhoola (1930-2019).

Biol Chem 2020 May 1. Epub 2020 May 1.

Universität Frankfurt, Frankfurt, Germany.

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http://dx.doi.org/10.1515/hsz-2020-0183DOI Listing

Small dense low-density lipoprotein-lowering agents.

Biol Chem 2019 Dec 1. Epub 2019 Dec 1.

Department of Medicine, Islamic Azad University, Qom Branch, Qom3714668669,Iran.

Metabolic disorders, including obesity, diabetes, and hyperlipidemia, as well as cardiovascular diseases (CVD), particularly atherosclerosis, are still leading causes of death worldwide. Plasma levels of low-density lipoprotein (LDL) are currently being considered as a critical risk factor for the diseases mentioned above, especially atherosclerosis. Because of the heterogeneous nature of LDL, many studies have already been conducted on its subclasses, especially small dense LDL (sdLDL). Read More

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http://dx.doi.org/10.1515/hsz-2019-0426DOI Listing
December 2019

HMGB3 silencing inhibits non-small cell lung cancer development through regulating WNT/β-catenin pathway.

Biol Chem 2020 May 1. Epub 2020 May 1.

Department of Thoracic surgery, PLA General Hospital, Beijing100853,China.

It has been reported that high-mobility group box3 (HMBG3) is overexpressed in various cancers. This study aimed to explore its function in non-small cell lung cancer (NSCLC). A546 and H460 cell lines were used for in vivo experiments, scratch healing tests, transwell migration and invasion experiments. Read More

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http://dx.doi.org/10.1515/hsz-2020-0144DOI Listing

Role of protein tyrosine phosphatase 1B (PTP1B) in the increased sensitivity of endothelial cells to a promigratory effect of erythropoietin in an inflammatory environment.

Biol Chem 2020 May 1. Epub 2020 May 1.

Universidad de Buenos Aires, Consejo Nacional de Investigaciones Científicas y Técnicas, Instituto del Departamento de Química Biológica de la Facultad de Ciencias Exactas y Naturales (IQUIBICEN), Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Ciudad Autónoma de Buenos AiresC1428EHA,Argentina.

The proliferation and migration of endothelial cells are vascular events of inflammation, a process which can also potentiate the effects of promigratory factors. With the aim of investigating possible modifications in the activity of erythropoietin (Epo) in an inflammatory environment, we found that Epo at a non-promigratory concentration was capable of stimulating EA.hy926 endothelial cell migration when TNF-α was present. Read More

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http://dx.doi.org/10.1515/hsz-2020-0136DOI Listing

Mitochondria and friends.

Biol Chem 2020 Apr 1. Epub 2020 Apr 1.

Institute of Biochemistry and Molecular Biology, University of Freiburg, Stefan-Meier- Straße 17, D-79104 Freiburg, Germany.

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http://dx.doi.org/10.1515/hsz-2020-0151DOI Listing

New horizons in mitochondrial contact site research.

Biol Chem 2020 Apr 1. Epub 2020 Apr 1.

Department of Molecular Genetics, Weizmann Institute of Science, Rehovot7610001,Israel.

Contact sites, areas where two organelles are held in close proximity through the action of molecular tethers, enable non-vesicular communication between compartments. Mitochondria have been center stage in the contact site field since the discovery of the first contact between mitochondria and the endoplasmic reticulum (ER) over 60 years ago. However, only now, in the last decade, has there been a burst of discoveries regarding contact site biology in general and mitochondrial contacts specifically. Read More

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http://dx.doi.org/10.1515/hsz-2020-0133DOI Listing

PINK1 and Parkin: team players in stress-induced mitophagy.

Biol Chem 2020 May 15. Epub 2020 May 15.

Department of Molecular Cell Biology, Institute of Biochemistry and Pathobiochemistry, Ruhr University Bochum, Universitätsstrasse 150, D-44801, Bochum, Germany.

Mitochondria are highly vulnerable organelles based on their complex biogenesis, entailing dependence on nuclear gene expression and efficient import strategies. They are implicated in a wide spectrum of vital cellular functions, including oxidative phosphorylation, iron-sulfur cluster synthesis, regulation of calcium homeostasis, and apoptosis. Moreover, damaged mitochondria can release mitochondrial components, such as mtDNA or cardiolipin, which are sensed as danger-associated molecular patterns and trigger innate immune signaling. Read More

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http://dx.doi.org/10.1515/hsz-2020-0135DOI Listing

Core 1 O-GalNAc glycosylation in the human cell nucleus.

Biol Chem 2019 Dec 1. Epub 2019 Dec 1.

Centro de Investigaciones en Química Biológica de Córdoba, CIQUIBIC, CONICET and Departamento de Química Biológica Ranwel Caputto, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Ciudad Universitaria, X5000HUA, Córdoba, Argentina.

Glycosylation is a very frequent post-translational modification in proteins, and the initiation of O-GalNAc glycosylation has been recently described on relevant nuclear proteins. Here we evaluated the nuclear incorporation of a second sugar residue in the biosynthesis pathway of O-GalNAc glycans to yield the terminal core 1 glycan (C1G, Galβ3GalNAcαSer/Thr). Using confocal microscopy, enzymatic assay, affinity chromatography and mass spectrometry, we analyzed intact cells, purified nuclei and soluble nucleoplasms to identify the essential factors for C1G biosynthesis in the cell nucleus. Read More

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http://dx.doi.org/10.1515/hsz-2019-0448DOI Listing
December 2019

Citrate synthase desuccinylation by SIRT5 promotes colon cancer cell proliferation and migration.

Biol Chem 2020 Apr 1. Epub 2020 Apr 1.

1Department of Medical Genetics, Center for Medical Genetics, Peking University Health Science Center, Beijing100191,China.

Citrate synthase (CS), the rate-limiting enzyme in the TCA cycle, catalyzes the first step of the cycle, namely, the condensation of oxaloacetate and acetyl-CoA to produce citrate. The expression and enzymatic activity of CS are altered in cancers, but posttranslational modification (PTM) of CS and its regulation in tumorigenesis remain largely obscure. SIRT5 belongs to the nicotinamide adenine dinucleotide (NAD)+-dependent deacetylase sirtuin family and plays vital roles in multiple biological processes via modulating various substrates. Read More

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http://dx.doi.org/10.1515/hsz-2020-0118DOI Listing

A role of heparan sulphate proteoglycan in the cellular uptake of lipocalins ß-lactoglobulin and allergen Fel d 4.

Biol Chem 2020 Mar 1. Epub 2020 Mar 1.

Institute of Molecular Biology, Medical University Innsbruck, Innrain 80, A-6020 Innsbruck, Austria.

Lipocalins, small extracellular hydrophobic molecule carriers, can be internalized by a variety of different cells. However, to date receptors have only been identified for human lipocalins. Here, we specifically investigated uptake mechanisms for lipocalins ß-lactoglobulin and Fel d 4 in HeLa and CHO cells. Read More

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http://dx.doi.org/10.1515/hsz-2020-0132DOI Listing

Vemurafenib downmodulates aggressiveness mediators of colorectal cancer (CRC): LMWPTP, PTP1B and TGFβ.

Biol Chem 2020 Mar 1. Epub 2020 Mar 1.

Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP), Monteiro Lobato Street 255, Campinas13083-862, SP,Brazil.

Colorectal Cancer (CRC) therapy confronts challenges as chemoresistance and side effects. Therefore, drugs with antitumor properties that downmodulate aggressiveness mediators are required. Studies have shown the relevance of Low Molecular Weight Tyrosine Phosphatase (LMWPTP), Protein Tyrosine Phosphatase 1B (PTP1B), and Transforming Growth Factor β (TGFβ) in mediating proliferation, chemoresistance, and metastasis. Read More

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http://dx.doi.org/10.1515/hsz-2020-0124DOI Listing

The MICOS complex, a structural element of mitochondria with versatile functions.

Biol Chem 2020 Mar 1. Epub 2020 Mar 1.

Institute of Cardiovascular Physiology and Pathophysiology, Biomedical Center, Ludwig- Maximilians University Munich, Großhaderner Str. 9, D-82152 Planegg/ Martinsried, Germany.

Mitochondria perform a plethora of functions in various cells of different tissues. Their architecture differs remarkably, for instance in neurons versus steroidogenic cells. Furthermore, aberrant mitochondrial architecture results in mitochondrial dysfunction. Read More

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http://dx.doi.org/10.1515/hsz-2020-0103DOI Listing

LncRNA ELFN1-AS1 promotes esophageal cancer progression by up-regulating GFPT1 via sponging miR-183-3p.

Biol Chem 2019 Dec 1. Epub 2019 Dec 1.

School of Life Sciences, Zhengzhou University, Zhengzhou 450001; Translational Medicine Center, Zhengzhou Central Hospital Affiliated to Zhengzhou University, Zhengzhou450007,China.

Accumulating studies highlight the critical role of long non-coding RNAs (lncRNAs) in the development of various human cancers. Extracellular leucine rich repeat and fibronectin type III domain containing 1-antisense RNA 1 (ELFN1-AS1) was shown to be a newfound lncRNA that abnormally expressed in human tumors. However, till now the specific function of this lncRNA in esophageal cancer (ESCA) remains unknown. Read More

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http://dx.doi.org/10.1515/hsz-2019-0430DOI Listing
December 2019

Lipid homeostasis in mitochondria.

Biol Chem 2020 Apr 22. Epub 2020 Apr 22.

Faculty of Life Sciences, Kyoto Sangyo University, Kamigamo-motoyama, Kita-ku, Kyoto 603-8555, Japan.

Mitochondria are surrounded by the two membranes, the outer and inner membranes, whose lipid compositions are optimized for proper functions and structural organizations of mitochondria. Although a part of mitochondrial lipids including their characteristic lipids, phosphatidylethanolamine and cardiolipin, are synthesized within mitochondria, their precursor lipids and other lipids are transported from other organelles, mainly the ER. Mitochondrially synthesized lipids are re-distributed within mitochondria and to other organelles, as well. Read More

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http://dx.doi.org/10.1515/hsz-2020-0121DOI Listing
April 2020
3.268 Impact Factor

From the discovery to molecular understanding of cellular iron-sulfur protein biogenesis.

Authors:
Roland Lill

Biol Chem 2020 Apr 17. Epub 2020 Apr 17.

Institut für Zytobiologie, Philipps-Universität Marburg, Robert-Koch-Str. 6, D-35032 Marburg, Germany.

Protein cofactors often are the business ends of proteins, and are either synthesized inside cells or are taken up from the nutrition. A cofactor that strictly needs to be synthesized by cells is the iron-sulfur (Fe/S) cluster. This evolutionary ancient compound performs numerous biochemical functions including electron transfer, catalysis, sulfur mobilization, regulation and protein stabilization. Read More

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http://dx.doi.org/10.1515/hsz-2020-0117DOI Listing

The piRNA pathway in planarian flatworms: new model, new insights.

Biol Chem 2020 Apr 29. Epub 2020 Apr 29.

Gene regulation by Non-coding RNA, Elite Network of Bavaria and University of Bayreuth, Universitätsstrasse 30, D-95447 Bayreuth, Germany.

PIWI-interacting RNAs (piRNAs) are small regulatory RNAs that associate with members of the PIWI clade of the Argonaute superfamily of proteins. piRNAs are predominantly found in animal gonads. There they silence transposable elements (TEs), regulate gene expression and participate in DNA methylation, thus orchestrating proper germline development. Read More

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http://dx.doi.org/10.1515/hsz-2019-0445DOI Listing

Comprehensive analysis of peptides and low molecular weight components of the giant ant Dinoponera quadriceps venom.

Biol Chem 2020 Mar 26. Epub 2020 Mar 26.

Institute of Natural Medicine, University of Toyama, Toyama 930-0194, Japan.

Ants (Hymenoptera, Apocrita, Aculeata, Formicoidea) comprise a well-succeeded group of animals. Like bees and wasps, ants are mostly venomous, having a sting system to deliver a mixture of bioactive organic compounds and peptides. The predatory giant ant Dinoponera quadriceps belongs to the subfamily Ponerinae that includes one of the largest known ant species in the world. Read More

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http://dx.doi.org/10.1515/hsz-2019-0397DOI Listing

Radiofrequency ablation triggers the migration of hepatocellular carcinoma cells by suppressing miR-148a-5p.

Biol Chem 2020 Mar 1. Epub 2020 Mar 1.

Department of Oncology, The Third People's Hospital of Zhengzhou, No.136 Nanshuncheng Street, Guangcheng District, Zhengzhou450000, Henan Province,China.

Increasing evidences suggested that insufficient radiofrequency ablation (IRFA) can paradoxically promote tumor invasion and metastatic processes, while the effects of moderate hyperthermia on cancer progression are not well illustrated. Our study found that IRFA can increase the in vitro migration, invasion and epithelial-mesenchymal transition (EMT) of hepatocellular carcinoma (HCC) cells via induction of Snail, a master regulator of EMT events. Among measured miRNAs, IRFA can decrease the expression of miR-148a-5p in HCC cells. Read More

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http://dx.doi.org/10.1515/hsz-2020-0130DOI Listing

Modular assembly of yeast mitochondrial ATP synthase and cytochrome oxidase.

Biol Chem 2020 May 6. Epub 2020 May 6.

Department of Biological Sciences, Columbia University, New York City, NY 10027, USA.

The respiratory pathway of mitochondria is composed of four electron transfer complexes and the ATP synthase. In this article, we review evidence from studies of Saccharomyces cerevisiae that both ATP synthase and cytochrome oxidase (COX) are assembled from independent modules that correspond to structurally and functionally identifiable components of each complex. Biogenesis of the respiratory chain requires a coordinate and balanced expression of gene products that become partner subunits of the same complex, but are encoded in the two physically separated genomes. Read More

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http://dx.doi.org/10.1515/hsz-2020-0112DOI Listing

From cytosol to mitochondria: the beginning of a protein journey.

Biol Chem 2020 Mar 25. Epub 2020 Mar 25.

Departamento de Genética Molecular, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Circuito Exterior s/n Ciudad Universitaria Coyoacán, México, Cd.Mx. 04510, Mexico.

Mitochondrial protein import is one of the key processes during mitochondrial biogenesis that involves a series of events necessary for recognition and delivery of nucleus-encoded/cytosol-synthesized mitochondrial proteins into the organelle. The past research efforts have mainly unraveled how membrane translocases ensure the correct protein sorting within the different mitochondrial subcompartments. However, early steps of recognition and delivery remain relatively uncharacterized. Read More

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http://dx.doi.org/10.1515/hsz-2020-0110DOI Listing

Protein import by the mitochondrial disulfide relay in higher eukaryotes.

Biol Chem 2020 Mar 25. Epub 2020 Mar 25.

Department of Chemistry, Institute for Biochemistry, Redox Biochemistry, University of Cologne, and Cologne Excellence Cluster on Cellular Stress Response in Aging-Associated Diseases, Zülpicher Str. 47a/R. 3.49, D-50674 Cologne, Germany.

The proteome of the mitochondrial intermembrane space (IMS) contains more than 100 proteins, all of which are synthesized on cytosolic ribosomes and consequently need to be imported by dedicated machineries. The mitochondrial disulfide relay is the major import machinery for soluble proteins in the IMS. Its major component, the oxidoreductase MIA40, interacts with incoming substrates, retains them in the IMS, and oxidatively folds them. Read More

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http://dx.doi.org/10.1515/hsz-2020-0108DOI Listing

How to get to the other side of the mitochondrial inner membrane - the protein import motor.

Authors:
Dejana Mokranjac

Biol Chem 2020 Mar 13. Epub 2020 Mar 13.

BMC-Physiological Chemistry, LMU Munich, Großhadernerstr. 9, D-82152 Planegg-Martinsried, Germany.

Biogenesis of mitochondria relies on import of more than 1000 different proteins from the cytosol. Approximately 70% of these proteins follow the presequence pathway - they are synthesized with cleavable N-terminal extensions called presequences and reach the final place of their function within the organelle with the help of the TOM and TIM23 complexes in the outer and inner membranes, respectively. The translocation of proteins along the presequence pathway is powered by the import motor of the TIM23 complex. Read More

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http://dx.doi.org/10.1515/hsz-2020-0106DOI Listing

The structure of the TOM core complex in the mitochondrial outer membrane.

Biol Chem 2020 Apr 1. Epub 2020 Apr 1.

University of Stuttgart, Institute of Biomaterials and Biomolecular Systems, Department of Biophysics, Pfaffenwaldring 57, D-70569Stuttgart, Germany.

In the past three decades, significant advances have been made in providing the biochemical background of TOM (translocase of the outer mitochondrial membrane)-mediated protein translocation into mitochondria. In the light of recent cryoelectron microscopy-derived structures of TOM isolated from Neurospora crassa and Saccharomyces cerevisiae, the interpretation of biochemical and biophysical studies of TOM-mediated protein transport into mitochondria now rests on a solid basis. In this review, we compare the subnanometer structure of N. Read More

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http://dx.doi.org/10.1515/hsz-2020-0104DOI Listing

Evolution of mitochondrial protein import - lessons from trypanosomes.

Authors:
André Schneider

Biol Chem 2020 Apr 7. Epub 2020 Apr 7.

Department of Chemistry and Biochemistry, University of Bern, Freiestrasse 3, CH-3012 Bern, Switzerland.

The evolution of mitochondrial protein import and the systems that mediate it marks the boundary between the endosymbiotic ancestor of mitochondria and a true organelle that is under the control of the nucleus. Protein import has been studied in great detail in Saccharomyces cerevisiae. More recently, it has also been extensively investigated in the parasitic protozoan Trypanosoma brucei, making it arguably the second best studied system. Read More

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http://dx.doi.org/10.1515/hsz-2019-0444DOI Listing

Targeting of parvulin interactors by diazirine mediated cross-linking discloses a cellular role of human Par14/17 in actin polymerization.

Biol Chem 2020 Apr 17. Epub 2020 Apr 17.

Structural and Medicinal Biochemistry, Center for Medical Biotechnology (ZMB), University of Duisburg-Essen, Universitätsstrasse 2-5, D-45117 Essen, Germany.

The peptidyl-prolyl cis/trans isomerases (PPIases) Parvulin 14 (Par14) and Parvulin 17 (Par17) result from alternative transcription initiation of the PIN4 gene. Whereas Par14 is present in all metazoan, Par17 is only expressed in Hominidae. Par14 resides mainly within the cellular nucleus, while Par17 is translocated into mitochondria. Read More

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http://dx.doi.org/10.1515/hsz-2019-0423DOI Listing

Redefining proteostasis transcription factors in organismal stress responses, development, metabolism, and health.

Biol Chem 2020 Apr 9. Epub 2020 Apr 9.

School of Molecular and Cell Biology and Astbury Centre for Structural Molecular Biology, Faculty of Biological Sciences, University of Leeds, Leeds LS2 9JT, UK.

Eukaryotic organisms have evolved complex and robust cellular stress response pathways to ensure maintenance of proteostasis and survival during fluctuating environmental conditions. Highly conserved stress response pathways can be triggered and coordinated at the cell-autonomous and cell-nonautonomous level by proteostasis transcription factors, including HSF1, SKN-1/NRF2, HIF1, and DAF-16/FOXO that combat proteotoxic stress caused by environmental challenges. While these transcription factors are often associated with a specific stress condition, they also direct "noncanonical" transcriptional programs that serve to integrate a multitude of physiological responses required for development, metabolism, and defense responses to pathogen infections. Read More

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http://dx.doi.org/10.1515/hsz-2019-0385DOI Listing

Regulation of mitochondrial plasticity by the i-AAA protease YME1L.

Biol Chem 2020 Feb 1. Epub 2020 Feb 1.

Max Planck Institute for Biology of Ageing, Joseph-Stelzmann-Str. 9b, D-50931 Cologne, Germany.

Mitochondria are multifaceted metabolic organelles and adapt dynamically to various developmental transitions and environmental challenges. The metabolic flexibility of mitochondria is provided by alterations in the mitochondrial proteome and is tightly coupled to changes in the shape of mitochondria. Mitochondrial proteases are emerging as important post-translational regulators of mitochondrial plasticity. Read More

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http://dx.doi.org/10.1515/hsz-2020-0120DOI Listing
February 2020

Comprehensive analysis of peptides and low molecular weight components of the giant ant Dinoponera quadriceps venom.

Biol Chem 2019 Dec 1. Epub 2019 Dec 1.

Institute of Natural Medicine, University of Toyama, Toyama, Japan.

Ants (Hymenoptera, Apocrita, Aculeata, Formicoidea) comprise a well-succeeded group of animals. Like bees and wasps, ants are mostly venomous, having a sting system to deliver a mixture of bioactive organic compounds and peptides. The predatory giant ant Dinoponera quadriceps belongs to the subfamily Ponerinae that include one of the largest known ant species in the world. Read More

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http://dx.doi.org/10.1515/hsz-2019-397ja-01DOI Listing
December 2019

From TOM to the TIM23 complex - handing over of a precursor.

Biol Chem 2020 Mar 9. Epub 2020 Mar 9.

Department of Cellular Biochemistry, University Medical Center Göttingen, D-37073 Göttingen, Germany.

Mitochondrial precursor proteins with amino-terminal presequences are imported via the presequence pathway, utilizing the TIM23 complex for inner membrane translocation. Initially, the precursors pass the outer membrane through the TOM complex and are handed over to the TIM23 complex where they are sorted into the inner membrane or translocated into the matrix. This handover process depends on the receptor proteins at the inner membrane, Tim50 and Tim23, which are critical for efficient import. Read More

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http://dx.doi.org/10.1515/hsz-2020-0101DOI Listing

The biogenesis of mitochondrial intermembrane space proteins.

Biol Chem 2020 Feb 1. Epub 2020 Feb 1.

Institute of Molecular Cell and Systems Biology, College of Medical, Veterinary and Life Sciences, University of Glasgow, University Avenue, GlasgowG12 8QQ, Scotland,UK.

The mitochondrial intermembrane space (IMS) houses a large spectrum of proteins with distinct and critical functions. Protein import into this mitochondrial sub-compartment is underpinned by an intriguing variety of pathways, many of which are still poorly understood. The constricted volume of the IMS and the topological segregation by the inner membrane cristae into a bulk area surrounded by the boundary inner membrane and the lumen within the cristae is an important factor that adds to the complexity of the protein import, folding and assembly processes. Read More

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http://dx.doi.org/10.1515/hsz-2020-0114DOI Listing
February 2020

Proteostasis in thermogenesis and obesity.

Biol Chem 2020 Mar 9. Epub 2020 Mar 9.

Department of Anatomy, Physiology and Pharmacology in the College of Medicine, University of Saskatchewan, 107 Wiggins Rd, Saskatchewan, S7N 5E5 Saskatoon, Canada.

The proper production, degradation, folding and activity of proteins, proteostasis, is essential for any cellular function. From single cell organisms to humans, selective pressures have led to the evolution of adaptive programs that ensure proteins are properly produced and disposed of when necessary. Environmental factors such as temperature, nutrient availability, pathogens as well as predators have greatly influenced the development of mechanisms such as the unfolded protein response, endoplasmic reticulum-associated protein degradation and autophagy, working together in concert to secure cellular proteostasis. Read More

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http://dx.doi.org/10.1515/hsz-2019-0427DOI Listing

The endoplasmic reticulum-mitochondria encounter structure: coordinating lipid metabolism across membranes.

Authors:
Benoît Kornmann

Biol Chem 2020 Mar 3. Epub 2020 Mar 3.

Department of Biochemistry, University of Oxford, South Parks Rd, Oxford OX1 3QU, UK.

Endosymbiosis, the beginning of a collaboration between an archaeon and a bacterium and a founding step in the evolution of eukaryotes, owes its success to the establishment of communication routes between the host and the symbiont to allow the exchange of metabolites. As far as lipids are concerned, it is the host that has learnt the symbiont's language, as eukaryote lipids appear to have been borrowed from the bacterial symbiont. Mitochondria exchange lipids with the rest of the cell at membrane contact sites. Read More

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http://dx.doi.org/10.1515/hsz-2020-0102DOI Listing

Small-molecule modulation of p53 protein-protein interactions.

Biol Chem 2020 Mar 7. Epub 2020 Mar 7.

Laboratory of Chemical Biology, Department of Biomedical Engineering and Institute for Complex Molecular Systems, Eindhoven University of Technology, NL-5600MB Eindhoven, The Netherlands.

Small-molecule modulation of protein-protein interactions (PPIs) is a very promising but also challenging area in drug discovery. The tumor suppressor protein p53 is one of the most frequently altered proteins in human cancers, making it an attractive target in oncology. 14-3-3 proteins have been shown to bind to and positively regulate p53 activity by protecting it from MDM2-dependent degradation or activating its DNA binding affinity. Read More

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http://dx.doi.org/10.1515/hsz-2019-0405DOI Listing

Heat shock protein 60 is a disease-associated sialoglycoprotein in human non-small cell lung cancer.

Biol Chem 2020 Mar 3. Epub 2020 Mar 3.

Department of Experimental Medicine and Biotechnology, PGIMER, Chandigarh 160012, India.

The diagnostic and therapeutic potential of Maackia amurensis agglutinin (MAA) have been reported in various malignancies. Earlier, we have found that MAA specifically interacted with human non-small cell lung-cancer (NSCLC) cells and induced apoptosis in these cells. The present study was designed to identify M. Read More

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http://dx.doi.org/10.1515/hsz-2019-0352DOI Listing

EZH2 function in immune cell development.

Biol Chem 2020 Feb 28. Epub 2020 Feb 28.

The Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville, Victoria 3052, Australia.

The polycomb repressive complex 2 (PRC2) consists of three core components EZH2, SUZ12 and EED. EZH2 catalyzes the methylation of lysine 27 of histone H3, a modification associated with gene silencing. Through gene duplication higher vertebrate genomes also encode a second partially redundant methyltransferase, EZH1. Read More

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http://dx.doi.org/10.1515/hsz-2019-0436DOI Listing
February 2020

Volatilomes of Cyclocybe aegerita during different stages of monokaryotic and dikaryotic fruiting.

Biol Chem 2020 Feb 11. Epub 2020 Feb 11.

Institute of Food Chemistry and Food Biotechnology, Justus Liebig University Giessen, D-35392 Giessen, Germany.

Volatile organic compounds (VOC) are characteristic for different fungal species. However, little is known about VOC changes during development and their biological role. Therefore, we established a laboratory cultivation system in modified crystallizing dishes for analyzing VOC during fruiting body development of the dikaryotic strain Cyclocybe aegerita AAE-3 as well as four monokaryotic offspring siblings exhibiting different fruiting phenotypes. Read More

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http://dx.doi.org/10.1515/hsz-2019-0392DOI Listing
February 2020

Biogenesis pathways of α-helical mitochondrial outer membrane proteins.

Biol Chem 2020 Feb 18. Epub 2020 Feb 18.

Interfaculty Institute of Biochemistry, University of Tübingen, Hoppe-Seyler-Str. 4, 72076 Tübingen, Germany.

Mitochondria harbor in their outer membrane (OM) proteins of different topologies. These proteins are encoded by the nuclear DNA, translated on cytosolic ribosomes and inserted into their target organelle by sophisticated protein import machineries. Recently, considerable insights have been accumulated on the insertion pathways of proteins into the mitochondrial OM. Read More

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http://dx.doi.org/10.1515/hsz-2019-0440DOI Listing
February 2020

Asymmetric inheritance of mitochondria in yeast.

Biol Chem 2020 Feb 18. Epub 2020 Feb 18.

Institut für Zellbiologie, Universität Bayreuth, 95440 Bayreuth, Germany.

Mitochondria are essential organelles of virtually all eukaryotic organisms. As they cannot be made de novo, they have to be inherited during cell division. In this review, we provide an overview on mitochondrial inheritance in Saccharomyces cerevisiae, a powerful model organism to study asymmetric cell division. Read More

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http://dx.doi.org/10.1515/hsz-2019-0439DOI Listing
February 2020

Porins as helpers in mitochondrial protein translocation.

Biol Chem 2019 Dec 1. Epub 2019 Dec 1.

Institute of Biochemistry und Molecular Biology, ZBMZ, Faculty of Medicine, University of Freiburg, 79104 Freiburg, Germany.

Mitochondria import the vast majority of their proteins via dedicated protein machineries. The translocase of the outer membrane (TOM complex) forms the main entry site for precursor proteins that are produced on cytosolic ribosomes. Subsequently, different protein sorting machineries transfer the incoming preproteins to the mitochondrial outer and inner membrane, the intermembrane space and the matrix. Read More

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http://dx.doi.org/10.1515/hsz-2019-0438DOI Listing
December 2019

What you see is what you get: activity-based probes in single-cell analysis of enzymatic activities.

Biol Chem 2020 Feb;401(2):233-248

Department of Chemical Biology (CBIO), Helmholtz-Centre for Infection Research, Inhoffenstr. 7, D-38102 Braunschweig, Germany.

Molecular imaging methods can provide spatio-temporal information about the distribution of biomolecules or biological processes, such as certain enzymatic activities, in single cells. Within a cell, it is possible to define the subcellular location of a target, its trafficking through the cell, colocalization with other biomolecules of interest and involvement in certain cell biological processes. On the other hand, single-cell imaging promises to distinguish cells that are phenotypically different from each other. Read More

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http://dx.doi.org/10.1515/hsz-2019-0262DOI Listing
February 2020

The redox status of cysteine thiol residues of apolipoprotein E impacts on its lipid interactions.

Biol Chem 2020 Apr;401(5):617-627

Department of Laboratory Medicine, Faculty of Medicine, University of Tsukuba, 1-1-1 Tennodai, Tsukuba 305-8575, Japan.

Redox-mediated modulation of cysteine (Cys) thiols has roles in various pathophysiological functions. We recently found that formation of disulfide-linked complexes of apolipoprotein (apo) E3 prevented apoE3 from irreversible oxidation. In this report, the influence of modification of Cys thiols in apoE2 and apoE3 on interactions with lipids was investigated. Read More

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http://dx.doi.org/10.1515/hsz-2019-0414DOI Listing

Human papillomavirus oncoproteins and post-translational modifications: generating multifunctional hubs for overriding cellular homeostasis.

Biol Chem 2020 Apr;401(5):585-599

International Centre for Genetic Engineering and Biotechnology, AREA Science Park, Padriciano 99, I-34149 Trieste, Italy.

Human papillomaviruses (HPVs) are major human carcinogens, causing around 5% of all human cancers, with cervical cancer being the most important. These tumors are all driven by the two HPV oncoproteins E6 and E7. Whilst their mechanisms of action are becoming increasingly clear through their abilities to target essential cellular tumor suppressor and growth control pathways, the roles that post-translational modifications (PTMs) of E6 and E7 play in the regulation of these activities remain unclear. Read More

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http://dx.doi.org/10.1515/hsz-2019-0408DOI Listing

Septin architecture and function in budding yeast.

Biol Chem 2020 Feb 6. Epub 2020 Feb 6.

Department of Biochemistry and Protein Structure, Institute of Molecular Biology SAS, Dubravska cesta 21, 84551 Bratislava, Slovak Republic.

The septins constitute a conserved family of guanosine phosphate-binding and filament-forming proteins widespread across eukaryotic species. Septins appear to have two principal functions. One is to form a cortical diffusion barrier, like the septin collar at the bud neck of Saccharomyces cerevisiae, which prevents movement of membrane-associated proteins between the mother and daughter cells. Read More

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http://dx.doi.org/10.1515/hsz-2019-0401DOI Listing
February 2020

Characterization of substrate specificity and novel autoprocessing mechanism of dipeptidase A from Prevotella intermedia.

Biol Chem 2020 Apr;401(5):629-642

Department of Oral Molecular Biology, Course of Medical and Dental Sciences, Nagasaki University Graduate School of Biomedical Sciences, 1-7-1 Sakamoto, Nagasaki 852-8588, Japan.

Prevotella intermedia, a Gram-negative anaerobic rod, is frequently observed in subgingival polymicrobial biofilms from adults with chronic periodontitis. Peptidases in periodontopathic bacteria are considered to function as etiological reagents. Prevotella intermedia OMA14 cells abundantly express an unidentified cysteine peptidase specific for Arg-4-methycoumaryl-7-amide (MCA). Read More

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http://dx.doi.org/10.1515/hsz-2019-0387DOI Listing

LncRNA MEG3 inhibits HMEC-1 cells growth, migration and tube formation via sponging miR-147.

Biol Chem 2020 Apr;401(5):601-615

Department of Vascular Surgery, China-Japan Union Hospital of Jilin University, No. 126 Xiantai Street, Changchun 130033, China.

Long non-coding RNA (lncRNA) maternally expressed gene 3 (MEG3) has been identified as a regulatory molecule in angiogenesis. The goal of this study was to illustrate how MEG3 affects the angiogenesis of vascular endothelial cells. Expression of MEG3, miR-147 and intracellular cell adhesion molecule-1 (ICAM-1) in human microvascular endothelial cell line (HMEC-1) was altered by transfection, then cell viability, apoptosis, migration, tube formation, as well as the correlation among MEG3, miR-147 and ICAM-1 were explored. Read More

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http://dx.doi.org/10.1515/hsz-2019-0230DOI Listing

Bioenergetics and translational metabolism: implications for genetics, physiology and precision medicine.

Biol Chem 2019 12;401(1):3-29

Department of Pathology, University of Alabama at Birmingham, 901 19th Street South, Birmingham, AL 35294, USA.

It is now becoming clear that human metabolism is extremely plastic and varies substantially between healthy individuals. Understanding the biochemistry that underlies this physiology will enable personalized clinical interventions related to metabolism. Mitochondrial quality control and the detailed mechanisms of mitochondrial energy generation are central to understanding susceptibility to pathologies associated with aging including cancer, cardiac and neurodegenerative diseases. Read More

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http://dx.doi.org/10.1515/hsz-2019-0268DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6944318PMC
December 2019

Power to the daughters - mitochondrial and mtDNA transmission during cell division.

Biol Chem 2020 Apr;401(5):533-546

Department of Biology II, Ludwig-Maximilians-University Munich, Großhaderner Str. 2, 82152 Planegg-Martinsried, Germany.

Mitochondria supply virtually all eukaryotic cells with energy through ATP production by oxidative phosphoryplation (OXPHOS). Accordingly, maintenance of mitochondrial function is fundamentally important to sustain cellular health and various diseases have been linked to mitochondrial dysfunction. Biogenesis of OXPHOS complexes crucially depends on mitochondrial DNA (mtDNA) that encodes essential subunits of the respiratory chain and is distributed in multiple copies throughout the mitochondrial network. Read More

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http://dx.doi.org/10.1515/hsz-2019-0337DOI Listing

Diffuse or hitch a ride: how photoreceptor lipidated proteins get from here to there.

Biol Chem 2020 Apr;401(5):573-584

Department of Ophthalmology and Visual Sciences, University of Utah Health Science Center, 65 Mario Capecchi Drive, Salt Lake City, UT 84132, USA.

Photoreceptors are polarized neurons, with specific subcellular compartmentalization and unique requirements for protein expression and trafficking. Each photoreceptor contains an outer segment (OS) where vision begins, an inner segment (IS) where protein synthesis occurs and a synaptic terminal for signal transmission to second-order neurons. The OS is a large, modified primary cilium attached to the IS by a slender connecting cilium (CC), the equivalent of the transition zone (TZ). Read More

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http://dx.doi.org/10.1515/hsz-2019-0375DOI Listing

Dr. NO and Mr. Toxic - the versatile role of nitric oxide.

Biol Chem 2020 Apr;401(5):547-572

Université Paris-Saclay, INRAE, AgroParisTech, Micalis Institute, 78350, Jouy-en-Josas, France.

Nitric oxide (NO) is present in various organisms from humans, to plants, fungus and bacteria. NO is a fundamental signaling molecule implicated in major cellular functions. The role of NO ranges from an essential molecule to a potent mediator of cellular damages. Read More

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http://dx.doi.org/10.1515/hsz-2019-0368DOI Listing

Different signaling and functionality of Rac1 and Rac1b in the progression of lung adenocarcinoma.

Biol Chem 2020 Mar;401(4):517-531

Molecular Oncology of Solid Tumors, Science Unit for Basic and Clinical Medicine, Justus Liebig University Giessen, Aulweg 128, D-35392 Giessen, Germany.

Rac1 is a ubiquitously expressed Rho GTPase and an important regulator of the actin cytoskeleton. Its splice variant Rac1b exhibits a 19-amino acid (aa) in-frame insertion and is predominantly active. Both proteins were described in tumorigenesis or metastasis. Read More

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http://dx.doi.org/10.1515/hsz-2019-0329DOI Listing