3,635 results match your criteria Biological chemistry[Journal]


Nm23-H1 inhibits hypoxia induced epithelial-mesenchymal transition and stemness in non-small cell lung cancer cells.

Biol Chem 2019 Feb 14. Epub 2019 Feb 14.

Jiangsu Province Hospital of Chinese Medicine, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing 210029, Jiangsu, China.

The Nm23 gene has been acknowledged to play a crucial role in lung cancer metastasis inhibitory cascades controlled by multiple factors. Low expression or allelic deletion of nm23-H1 is strongly linked to widespread metastasis and poor differentiation of non-small cell lung cancer (NSCLC). In this study, nm23-H1 was down regulated in epithelial-mesenchymal transition (EMT) and stemness enhancement under cobalt chloride (CoCl2)-induced hypoxia in NSCLC cells. Read More

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http://dx.doi.org/10.1515/hsz-2018-0351DOI Listing
February 2019

Characterization and engineering of photoactivated adenylyl cyclases.

Biol Chem 2019 Feb;400(3):429-441

Lehrstuhl für Biochemie, Universität Bayreuth, D-95447 Bayreuth, Germany.

Cyclic nucleoside monophosphates (cNMP) serve as universal second messengers in signal transduction across prokaryotes and eukaryotes. As signaling often relies on transiently formed microdomains of elevated second messenger concentration, means to precisely perturb the spatiotemporal dynamics of cNMPs are uniquely poised for the interrogation of the underlying physiological processes. Optogenetics appears particularly suited as it affords light-dependent, accurate control in time and space of diverse cellular processes. Read More

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http://dx.doi.org/10.1515/hsz-2018-0375DOI Listing
February 2019

Sequence selection by FitSS4ASR alleviates ancestral sequence reconstruction as exemplified for geranylgeranylglyceryl phosphate synthase.

Biol Chem 2019 Feb;400(3):367-381

Institute of Biophysics and Physical Biochemistry, University of Regensburg, Universitätsstraße 31, D-93040 Regensburg, Germany.

For evolutionary studies, but also for protein engineering, ancestral sequence reconstruction (ASR) has become an indispensable tool. The first step of every ASR protocol is the preparation of a representative sequence set containing at most a few hundred recent homologs whose composition determines decisively the outcome of a reconstruction. A common approach for sequence selection consists of several rounds of manual recompilation that is driven by embedded phylogenetic analyses of the varied sequence sets. Read More

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http://dx.doi.org/10.1515/hsz-2018-0344DOI Listing
February 2019

Highly flexible, IgG-shaped, trivalent antibodies effectively target tumor cells and induce T cell-mediated killing.

Biol Chem 2019 Feb;400(3):343-350

Roche Pharma Research and Early Development, Large Molecule Research, Roche Innovation Center Munich, Nonnenwald 2, D-82377 Penzberg, Germany.

A novel bispecific antibody format was applied to generate T cell-engaging antibodies. The TriFab format is a trivalent IgG-shaped entity composed of two Fab arms that bind to antigens on the surface of tumor cells, which are linked via flexible peptides to a CD3 binding moiety that replaces the CH2 domains of conventional IgGs. The distinctive feature of these T cell recruiting bispecifics is that their CD3 variable regions are incorporated between domains, rather than N- or C-terminally fused to an Fc or antibody fragments. Read More

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http://dx.doi.org/10.1515/hsz-2018-0338DOI Listing
February 2019

CFTR structure, stability, function and regulation.

Biol Chem 2018 Dec 1. Epub 2018 Dec 1.

School of Biological Sciences, Faculty of Biology Medicine and Health, Michael Smith Building, The University of Manchester, Oxford Road, ManchesterM13 9PL,UK.

CFTR (Cystic Fibrosis transmembrane conductance regulator) is a unique member of the ATP-binding cassette family of proteins because it has evolved into a channel. Mutations in CFTR cause cystic fibrosis, the most common genetic disease in people of European origins. The F508del mutation is found in about 90% of patients and here we present data that suggest its main effect is on CFTR stability rather than on the 3D folded state. Read More

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http://dx.doi.org/10.1515/hsz-2018-0470DOI Listing
December 2018
1 Read

LncRNA HOTAIR targets miR-126-5p to promote the progression of Parkinson's disease through RAB3IP.

Biol Chem 2018 Dec 1. Epub 2018 Dec 1.

Department of Organic Chemistry, College of Chemistry, Jilin University, No. 2699 Qianjin Street, Changchun 130012, Jilin, China.

Parkinson's disease (PD) is a common neurological disorder characterized by dopaminergic (DA) neuron degeneration and death in the midbrain, and the long noncoding RNA HOTAIR has been shown to affect disease progression in PD. In this study, we aimed to further illustrate the molecular mechanism of HOTAIR in PD. Bioinformatics analysis was utilized to determine the potential downstream targets of HOTAIR in PD. Read More

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http://dx.doi.org/10.1515/hsz-2018-0431DOI Listing
December 2018

Metalloprotease inhibitor profiles of human ADAM8 in vitro and in cell-based assays.

Biol Chem 2018 Dec 1. Epub 2018 Dec 1.

Department of Neurosurgery, University of Marburg, Baldingerstrasse, D-35033 Marburg, Germany.

ADAM8 as a membrane-anchored metalloproteinase-disintegrin is upregulated under pathological conditions such as inflammation and cancer. As active sheddase, ADAM8 can cleave several membrane proteins, among them the low-affinity receptor FcεRII CD23. Hydroxamate-based inhibitors are routinely used to define relevant proteinases involved in ectodomain shedding of membrane proteins. Read More

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http://www.degruyter.com/view/j/bchm.just-accepted/hsz-2018-
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http://dx.doi.org/10.1515/hsz-2018-0396DOI Listing
December 2018
1 Read

Zinc-induced activation of GPR39 regulates glucose homeostasis through glucose-dependent insulinotropic polypeptide secretion from enteroendocrine K-cells.

Biol Chem 2018 Dec 1. Epub 2018 Dec 1.

School of Biomedical Sciences, Ulster University, Cromore Road, ColeraineBT52 1SA, NorthernIreland.

The role of Zn2+-sensing receptor GPR39 on glucose homeostasis and incretin regulation was assessed in enteroendocrine L- and K-cells. Anti-hyperglycaemic, insulinotropic and incretin secreting properties of Zn2+ were explored in normal, diabetic and incretin receptor knockout mice. Compared to intraperitoneal injection, oral administration of Zn2+ (50 μmol/kg body weight) with glucose (18 mmol/kg) in lean mice reduced the glycaemic excursion by 25-34% (p<0. Read More

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http://dx.doi.org/10.1515/hsz-2018-0393DOI Listing
December 2018
1 Read

Molecular effects of dietary fatty acids on brain insulin action and mitochondrial function.

Biol Chem 2018 Dec 1. Epub 2018 Dec 1.

Central Regulation of Metabolism, German Institute of Human Nutrition (DIfE), Arthur- Scheunert-Allee 114-116, D-14558 Nuthetal, Germany.

The prevalence of obesity and its co-morbidities such as insulin resistance and type 2 diabetes are tightly linked to increased ingestion of palatable fat enriched food. Thus, it seems intuitive that the brain senses elevated amounts of fatty acids and affects adaptive metabolic response, which is connected to mitochondrial function and insulin signaling. This review will address the effect of dietary fatty acids on brain insulin and mitochondrial function with a special emphasis on the impact of different fatty acids on brain function and metabolism. Read More

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http://dx.doi.org/10.1515/hsz-2018-0477DOI Listing
December 2018

ABCB4 / MDR3 in health and disease - at the crossroads of biochemistry and medicine.

Biol Chem 2018 Dec 1. Epub 2018 Dec 1.

Institute of Biochemistry, Heinrich Heine University Düsseldorf, Universitätsstraße 1, D-40225 Düsseldorf, Germany.

Several ABC transporters of the human liver are responsible for the secretion of bile salts, lipids and cholesterol. Their interplay protects the biliary tree from the harsh detergent activity of bile salts. Among these transporters, ABCB4 is essential for the translocation of phosphatidylcholine lipids from the inner to the outer leaflet of the canalicular membrane of hepatocytes. Read More

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http://dx.doi.org/10.1515/hsz-2018-0441DOI Listing
December 2018
1 Read

Protein engineering comes of age.

Authors:
Harald Kolmar

Biol Chem 2019 Feb;400(3):255-256

Applied Biochemistry, Technische Universität Darmstadt, Alarich-Weiss-Str.-4, D-64287 Darmstadt, Germany.

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http://dx.doi.org/10.1515/hsz-2019-0108DOI Listing
February 2019

MCT1, MCT4 and CD147 expression and 3-bromopyruvate toxicity in colorectal cancer cells are modulated by the extracellular conditions.

Biol Chem 2019 Feb 14. Epub 2019 Feb 14.

CESPU, Instituto de Investigação e Formação Avançada em Ciências e Tecnologias da Saúde, Rua Central de Gandra, 1317, 4585-116, Gandra, PRD, Portugal.

Monocarboxylate transporters (MCTs) inhibition leads to disruption in glycolysis, induces cell death and decreases cell invasion, revealing the importance of MCT activity in intracellular pH homeostasis and tumor aggressiveness. 3-Bromopyruvate (3BP) is an anti-tumor agent, whose uptake occurs via MCTs. It was the aim of this work to unravel the importance of extracellular conditions on the regulation of MCTs and in 3BP activity. Read More

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http://dx.doi.org/10.1515/hsz-2018-0411DOI Listing
February 2019

Nodal promotes the malignancy of non-small cell lung cancer (NSCLC) cells via activation of NF-κB/IL-6 signals.

Biol Chem 2018 Dec 1. Epub 2018 Dec 1.

Department of Thoracic Surgery, Peking Union Medical College Hospital, Shuaifuyuan No.1, Dongcheng District, Beijing100730,China.

Non-small cell lung cancer (NSCLC) is one of the leading causes of cancer deaths worldwide. Understanding the mechanisms responsible for the malignancy of NSCLC cell is important for therapy and drug development. Nodal, an important embryonic morphogen, has been reported to modulate tumorigenesis. Read More

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http://dx.doi.org/10.1515/hsz-2018-0392DOI Listing
December 2018

Computational design of structured loops for new protein functions.

Biol Chem 2019 Feb;400(3):275-288

Graduate Group in Biophysics, University of California San Francisco, San Francisco, CA 94158, USA.

The ability to engineer the precise geometries, fine-tuned energetics and subtle dynamics that are characteristic of functional proteins is a major unsolved challenge in the field of computational protein design. In natural proteins, functional sites exhibiting these properties often feature structured loops. However, unlike the elements of secondary structures that comprise idealized protein folds, structured loops have been difficult to design computationally. Read More

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http://dx.doi.org/10.1515/hsz-2018-0348DOI Listing
February 2019

Hit evaluation of an α-helical peptide: Ala-scan, truncation and sidechain-to-sidechain macrocyclization of an RNA polymerase Inhibitor.

Biol Chem 2019 Feb;400(3):333-342

Helmholtz Institute for Pharmaceutical Research Saarland (HIPS) - Helmholtz Centre for Infection Research (HZI), Department of Drug Design and Optimization, Campus E8.1, D-66123 Saarbrücken, Germany.

RNA polymerase (RNAP) remains a relatively underexplored target with only rifampicin and fidaxomicin in clinical use. Hence, the concurrent rise in bacterial resistance rate urges the search for novel RNAP inhibitors with a novel mode of action. In this work, we investigated the impact of several systematic modifications including sidechain-to-sidechain macrocylization in the α-helical content and biological activity of a previously identified inhibitory sigma factor fragment. Read More

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http://dx.doi.org/10.1515/hsz-2018-0333DOI Listing
February 2019
2 Reads

Organic anion transporters 1 and 3 influence cellular energy metabolism in renal proximal tubule cells.

Biol Chem 2019 Jan 29. Epub 2019 Jan 29.

Department of Pharmacology and Toxicology, Radboud Institute for Molecular Life Sciences, Radboud University Medical Center, P.O. Box 9101, NL-6500HB, Nijmegen, The Netherlands.

Organic anion transporters (OATs) 1 and 3 are, besides being uptake transporters, key in several cellular metabolic pathways. The underlying mechanisms are largely unknown. Hence, we used human conditionally immortalized proximal tubule epithelial cells (ciPTEC) overexpressing OAT1 or OAT3 to gain insight into these mechanisms. Read More

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http://dx.doi.org/10.1515/hsz-2018-0446DOI Listing
January 2019
1 Read

The conserved theme of ribosome hibernation: from bacteria to chloroplasts of plants.

Biol Chem 2019 Feb 1. Epub 2019 Feb 1.

Department of Biology, Molecular Genetics of Eukaryotes, University of Kaiserslautern, Paul-Ehrlich-Straße 23, D-67663 Kaiserslautern, Germany.

Cells are highly adaptive systems that respond and adapt to changing environmental conditions such as temperature fluctuations or altered nutrient availability. Such acclimation processes involve reprogramming of the cellular gene expression profile, tuning of protein synthesis, remodeling of metabolic pathways and morphological changes of the cell shape. Nutrient starvation can lead to limited energy supply and consequently, remodeling of protein synthesis is one of the key steps of regulation since the translation of the genetic code into functional polypeptides may consume up to 40% of a cell's energy during proliferation. Read More

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http://dx.doi.org/10.1515/hsz-2018-0436DOI Listing
February 2019
12 Reads

Chemical modification of neuropeptide Y for human Y1 receptor targeting in health and disease.

Biol Chem 2019 Feb;400(3):299-311

Institute of Biochemistry, Faculty of Life Sciences, Leipzig University, Brüderstr. 34, D-04103 Leipzig, Germany.

As a very abundant neuropeptide in the brain and widely distributed peptide hormone in the periphery, neuropeptide Y (NPY) appears to be a multisignaling key peptide. Together with peptide YY, pancreatic polypeptide and the four human G protein-coupled receptor subtypes hY1R, hY2R, hY4R and hY5R it forms the NPY/hYR multiligand/multireceptor system, which is involved in essential physiological processes as well as in human diseases. In particular, NPY-induced hY1R signaling plays a central role in the regulation of food intake and stress response as well as in obesity, mood disorders and cancer. Read More

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http://dx.doi.org/10.1515/hsz-2018-0364DOI Listing
February 2019
6 Reads

Changqin NO. 1 inhibits neuronal apoptosis via suppressing GAS5 expression in traumatic brain injury mice model.

Biol Chem 2018 Dec 1. Epub 2018 Dec 1.

Department of Integrated Traditional Chinese and Western Medicine, Xiangya Hospital, Central South University, 87 Xiangya Road, Changsha 410078, Hunan, China.

The present study was designed to investigate the mechanism of traditional Chinese medicine Changqin NO. 1 on amelioration of traumatic brain injury (TBI). Adult male C57BL/6J mice and newborn mice were used to generate a mouse TBI model and harvest primary neurons, respectively. Read More

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http://dx.doi.org/10.1515/hsz-2018-0340DOI Listing
December 2018
3 Reads

Kinetically selective and potent inhibitors of HDAC8.

Biol Chem 2018 Dec 1. Epub 2018 Dec 1.

Department of Chemical Engineering and Biotechnology, University of Applied Sciences Darmstadt, 64295 Darmstadt, Germany.

HDAC8 is an established and validated target for T-cell lymphoma and childhood neuroblastoma. The active site binding pocket of HDAC8 is highly conserved among all zinccontaining representatives of the histone deacetylase family. This explains that most HDACs are unselectively recognized by similar inhibitors featuring a zinc binding group (ZBG), a hydrophobic linker and a head group. Read More

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http://dx.doi.org/10.1515/hsz-2018-0363DOI Listing
December 2018
7 Reads

Directed evolution of the 3C protease from coxsackievirus using a novel fluorescence-assisted intracellular method.

Biol Chem 2019 Feb;400(3):405-415

Department of Protein Science, School of Engineering Sciences in Chemistry, Biotechnology and Health, KTH Royal Institute of Technology, SE-106 91 Stockholm, Sweden.

Proteases are crucial for regulating biological processes in organisms through hydrolysis of peptide bonds. Recombinant proteases have moreover become important tools in biotechnological, and biomedical research and as therapeutics. We have developed a label-free high-throughput method for quantitative assessment of proteolytic activity in Escherichia coli. Read More

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http://dx.doi.org/10.1515/hsz-2018-0362DOI Listing
February 2019
11 Reads

LncRNA TINCR / microRNA-107 / CD36 regulates cell proliferation and apoptosis in colorectal cancer via PPAR signaling pathway based on bioinformatics analysis.

Biol Chem 2018 Dec 1. Epub 2018 Dec 1.

Department of Gastroenterology, the First Affiliated Hospital of Zhengzhou University, Zhengzhou Henan, 450052,China.

Present study aims to determine the potential biomarkers and uncover the regulatory mechanisms of lncRNA TINCR / miR-107 / CD36 axis in CRC. Aberrantly expressed lncRNAs and differential-expressed genes were identified by analyzing the dataset GSE40967. Gene set enrichment analysis were employed, and Cytoscape software helps establishing the co-expression network between lncRNAs and genes. Read More

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http://dx.doi.org/10.1515/hsz-2018-0236DOI Listing
December 2018
2 Reads

The comparative biochemistry of viruses and humans: an evolutionary path towards autoimmunity.

Authors:
Darja Kanduc

Biol Chem 2018 Dec 3. Epub 2018 Dec 3.

Department of Biosciences, Biotechnologies and Biopharmaceutics, University of Bari, Via Orabona 4, I-70124 Bari, Italy.

Analyses of the peptide sharing between five common human viruses (Borna disease virus, influenza A virus, measles virus, mumps virus and rubella virus) and the human proteome highlight a massive viral vs. human peptide overlap that is mathematically unexpected. Evolutionarily, the data underscore a strict relationship between viruses and the origin of eukaryotic cells. Read More

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http://www.degruyter.com/view/j/bchm.ahead-of-print/hsz-2018
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http://dx.doi.org/10.1515/hsz-2018-0271DOI Listing
December 2018
14 Reads

Peptide binding affinity redistributes preassembled repeat protein fragments.

Biol Chem 2019 Feb;400(3):395-404

Department of Chemistry, University of Zurich, Winterthurerstrasse 190, CH-8057 Zürich, Switzerland.

Designed armadillo repeat proteins (dArmRPs) are modular peptide binders composed of N- and C-terminal capping repeats Y and A and a variable number of internal modules M that each specifically recognize two amino acids of the target peptide. Complementary fragments of dArmRPs obtained by splitting the protein between helices H1 and H2 of an internal module show conditional and specific assembly only in the presence of a target peptide (Michel, E., Plückthun, A. Read More

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http://dx.doi.org/10.1515/hsz-2018-0355DOI Listing
February 2019
1 Read

Differential expression of DLG1 as a common trait in different human diseases: an encouraging issue in molecular pathology.

Biol Chem 2018 Dec 1. Epub 2018 Dec 1.

Instituto de Biología Molecular y Celular de Rosario-CONICET, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Suipacha, Rosario, Argentina.

Human Disc large (DLG1) is a scaffolding protein that through the interaction with diverse cell partners participates in the control of key cellular processes such as polarity, proliferation and migration. Experimental data have mainly identified DLG1 as a tumor suppressor. An outstanding point for DLG1 protein is that altered DLG1 expression and DLG1 gene mutations were observed in different pathologies, including cancer and neurological and immunological disorders. Read More

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http://dx.doi.org/10.1515/hsz-2018-0350DOI Listing
December 2018
2 Reads

An engineered lipocalin that tightly complexes the plant poison colchicine for use as antidote and in bioanalytical applications.

Biol Chem 2019 Feb;400(3):351-366

Munich Center for Integrated Protein Science (CIPS-M) and Lehrstuhl für Biologische Chemie, Technische Universität München, 85354 Freising (Weihenstephan), Germany.

Colchicine is a toxic alkaloid prevalent in autumn crocus (Colchicum autumnale) that binds to tubulin and inhibits polymerization of microtubules. Using combinatorial and rational protein design, we have developed an artificial binding protein based on the human lipocalin 2 that binds colchicine with a dissociation constant of 120 pm, i.e. Read More

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http://www.degruyter.com/view/j/bchm.just-accepted/hsz-2018-
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http://dx.doi.org/10.1515/hsz-2018-0342DOI Listing
February 2019
5 Reads

Shining light on plant hormones with genetically encoded biosensors.

Biol Chem 2018 Dec 4. Epub 2018 Dec 4.

Laboratoire Reproduction et Développement des Plantes, Université Lyon, ENS de Lyon, UCB Lyon 1, CNRS, INRA, F-69342 Lyon, France.

Signalling molecules are produced, degraded, modified and transported throughout the development of higher organisms. Understanding their mode of action implies understanding these dynamics in vivo and in real time. Genetically encoded biosensors are being more and more used as tools to 'follow' signalling molecules and their responses inside an organism. Read More

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http://www.degruyter.com/view/j/bchm.ahead-of-print/hsz-2018
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http://dx.doi.org/10.1515/hsz-2018-0310DOI Listing
December 2018
13 Reads

Conversion of chenodeoxycholic acid to cholic acid by human CYP8B1.

Biol Chem 2018 Nov 1. Epub 2018 Nov 1.

School of Pharmaceutical Science and Technology, Health Sciences Platform, Tianjin University, Tianjin30072,China.

The human cytochrome P450 enzyme CYP8B1 is a crucial regulator of the balance of cholic acid (CA) and chenodeoxycholic acid (CDCA) in the liver. It was previously shown to catalyze the conversion of 7α-hydroxycholest-4-en-3-one, a CDCA precursor, to 7α,12α- dihydroxycholest-4-en-3-one, which is an intermediate of CA biosynthesis. In this study we demonstrate that CYP8B1 can also convert CDCA itself to CA. Read More

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http://dx.doi.org/10.1515/hsz-2018-0379DOI Listing
November 2018
14 Reads

Facile generation of antibody heavy and light chain diversities for yeast surface display by Golden Gate Cloning.

Biol Chem 2019 Feb;400(3):383-393

Protein Engineering and Antibody Technologies, Merck KGaA, Frankfurter Strasse 250, D-64293 Darmstadt, Germany.

Antibodies can be successfully engineered and isolated by yeast or phage display of combinatorial libraries. Still, generation of libraries comprising heavy chain as well as light chain diversities is a cumbersome process involving multiple steps. Within this study, we set out to compare the output of yeast display screening of antibody Fab libraries from immunized rodents that were generated by Golden Gate Cloning (GGC) with the conventional three-step method of individual heavy- and light-chain sub-library construction followed by chain combination via yeast mating (YM). Read More

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http://dx.doi.org/10.1515/hsz-2018-0347DOI Listing
February 2019
23 Reads

Unforgettable force - crosstalk and memory of mechanosensitive structures.

Biol Chem 2018 Nov 1. Epub 2018 Nov 1.

Group of Molecular Mechanotransduction, Max Planck Institute of Biochemistry, 82152 Martinsried, Germany.

The ability of cells to sense and respond to mechanical stimuli is crucial for many developmental and homeostatic processes, while mechanical dysfunction of cells has been associated with numerous pathologies including muscular dystrophies, cardiovascular defects, and epithelial disorders. Yet, how cells detect and process mechanical information is still unclear. In this review, we outline major mechanisms underlying cellular mechanotransduction and we summarize the current understanding of how cells integrate information from distinct mechanosensitive structures to mediate complex mechanoresponses. Read More

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http://dx.doi.org/10.1515/hsz-2018-0328DOI Listing
November 2018
1 Read

Synthetic biology of B cell activation: understanding signal amplification at the B cell antigen receptor using a rebuilding approach.

Biol Chem 2018 Nov 1. Epub 2018 Nov 1.

BIOSS Centre for Biological Signalling Studies, Department of Molecular Immunology, Institute of Biology III, Faculty of Biology, University of Freiburg, Freiburg, Germany.

Upon activation of the B cell antigen receptor (BCR), the spleen tyrosine kinase (Syk) and the Src family kinase Lyn phosphorylate tyrosines of the immunoreceptor tyrosine-based activation motif (ITAM) of Igα and Igβ which further serve as binding sites for the SH2 domains of these kinases. Using a synthetic biology approach, we dissect the roles of different ITAM residues of Igα in Syk activation. We find that a leucine to glycine mutation at the Y+3 position after the first ITAM tyrosine prevents Syk binding and activation. Read More

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http://dx.doi.org/10.1515/hsz-2018-0308DOI Listing
November 2018
10 Reads

Highlight Issue: Plasma Medicine.

Biol Chem 2018 Dec;400(1):1-2

Leibniz Institute for Plasma Science and Technology (INP Greifswald), Felix-Hausdorff-Straße 2, D-17489 Greifswald, Germany.

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http://dx.doi.org/10.1515/hsz-2018-0410DOI Listing
December 2018
1 Read

Rational design of an improved photo-activatable intein for the production of head-to-tail cyclized peptides.

Biol Chem 2019 Feb;400(3):417-427

Department of Chemistry and Pharmacy, Institute of Biochemistry, University of Münster, Wilhelm-Klemm-Str. 2, D-48149 Münster, Germany.

Head-to-tail cyclization of genetically encoded peptides and proteins can be achieved with the split intein circular ligation of peptides and proteins (SICLOPPS) method by inserting the desired polypeptide between the C- and N-terminal fragments of a split intein. To prevent the intramolecular protein splicing reaction from spontaneously occurring upon folding of the intein domain, we have previously rendered this process light-dependent in a photo-controllable variant of the M86 intein, using genetically encoded ortho-nitrobenzyltyrosine at a structurally important position. Here, we report improvements on this photo-intein with regard to expression yields and rate of cyclic peptide formation. Read More

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http://www.degruyter.com/view/j/bchm.just-accepted/hsz-2018-
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http://dx.doi.org/10.1515/hsz-2018-0367DOI Listing
February 2019
3 Reads

Chemistry and biochemistry of cold physical plasma derived reactive species in liquids.

Biol Chem 2018 Dec;400(1):19-38

ZIK Plasmatis, Leibniz-Institute for Plasma Science and Technology, Felix-Hausdorff-Str. 2, D-17489 Greifswald, Germany.

Reactive oxygen and nitrogen species deposited by cold physical plasma are proposed as predominant effectors in the interaction between discharge and biomedical application. Most reactive species found in plasma sources are known in biology for inter- and intracellular communication (redox signaling) and mammalian cells are equipped to interpret the plasma derived redox signal. As such, considerable effort has been put into the investigation of potential clinical applications and the underlying mechanism, with a special emphasis on conditions orchestrated significantly via redox signaling. Read More

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http://www.degruyter.com/view/j/bchm.ahead-of-print/hsz-2018
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http://dx.doi.org/10.1515/hsz-2018-0242DOI Listing
December 2018
7 Reads

Stimulation of melanin synthesis in melanoma cells by cold plasma.

Biol Chem 2018 Dec;400(1):101-109

Department Plasma Life Science, Leibniz-Institute for Plasma Science and Technology (INP), Felix-Hausdorff-Str. 2, D-17489 Greifswald, Germany.

Skin color is derived from epidermal melanocytes that contain specialized organelles in which melanin is formed. The formation of melanin is a well-orchestrated process, and reactive oxygen species (ROS) play a role in numerous enzymatic conversions, such as the reactions catalyzed by tyrosinase and tyrosine hydroxylase. Currently, there is ample evidence that cold plasma exerts biological effects on cells through the impact of ROS and reactive nitrogen species (RNS). Read More

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http://www.degruyter.com/view/j/bchm.ahead-of-print/hsz-2018
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http://dx.doi.org/10.1515/hsz-2018-0223DOI Listing
December 2018
20 Reads

MiR-23a-3p-regulated abnormal acetylation of FOXP3 induces regulatory T cell function defect in Graves' disease.

Biol Chem 2018 Dec 3. Epub 2018 Dec 3.

Department of General surgery, The First Affiliated Hospital of Zhengzhou University, No. 41 Jianshe Road, Erqi District, Zhengzhou 450052, Henan Province, China.

This study aims to investigate the mechanism of miR-23a-3p in regulating Treg dysfunction in Graves' disease (GD). The percentage of Treg cells and interleukin (IL)-17+ T cells were determined by flow cytometry. The expression of forkhead box P3 (FOXP3), sirtuin 1 (SIRT1), RAR-related orphan receptor gamma t (RORγt) and miR-23a-3p was analyzed by quantitative reverse transcription polymerase chain reaction (qRT-PCR) or Western blot. Read More

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http://www.degruyter.com/view/j/bchm.just-accepted/hsz-2018-
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http://dx.doi.org/10.1515/hsz-2018-0343DOI Listing
December 2018
11 Reads

Differential involvement of TAK1, RIPK1 and NF-κB signaling in Smac mimetic-induced cell death in breast cancer cells.

Biol Chem 2019 Jan;400(2):171-180

Institute for Experimental Cancer Research in Pediatrics, Goethe University, Komturstrasse 3a, D-60528 Frankfurt/Main, Germany.

Smac mimetics (SMs) are considered promising cancer therapeutics. However, the mechanisms responsible for mediating cell death by SMs are still only partly understood. Therefore, in this study, we investigated signaling pathways upon treatment with the bivalent SM BV6 using two SM-sensitive breast cancer cell lines as models. Read More

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http://www.degruyter.com/view/j/bchm.ahead-of-print/hsz-2018
Publisher Site
http://dx.doi.org/10.1515/hsz-2018-0324DOI Listing
January 2019
2 Reads

Regulatory effect of hsa-miR-5590-3P on TGFβ signaling through targeting of TGFβ-R1, TGFβ-R2, SMAD3 and SMAD4 transcripts.

Biol Chem 2018 Nov 29. Epub 2018 Nov 29.

Department of Molecular Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran 1411713116, Iran.

Transforming growth factor-β (TGFβ) signaling acts as suppressor and inducer of tumor progression during the early and late stages of cancer, respectively. Some miRNAs have shown a regulatory effect on TGFβ signaling and here, we have used a combination of bioinformatics and experimental tools to show that hsa-miR-5590-3p is a regulator of multiple genes expression in the TGFβ signaling pathway. Consistent with the bioinformatics predictions, hsa-miR-5590-3p had a negative correlation of expression with TGFβ-R1, TGFβ-R2, SMAD3 and SMAD4 genes, detected by reverse transcription-quantitative polymerase chain reaction (RT-qPCR). Read More

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http://www.degruyter.com/view/j/bchm.just-accepted/hsz-2018-
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http://dx.doi.org/10.1515/hsz-2018-0264DOI Listing
November 2018
3 Reads

Refolding and in vitro characterization of human papillomavirus 16 minor capsid protein L2.

Biol Chem 2018 Oct 30. Epub 2018 Oct 30.

Institute of Medical Virology, University of Tübingen, Elfriede-Aulhorn-Str. 06, D-72076 Tuebingen, Germany.

The minor capsid protein L2 of papillomaviruses exhibits multiple functions during viral entry including membrane interaction. Information on the protein is scarce, because of its high tendency of aggregation. We determined suitable conditions to produce a functional human papillomavirus (HPV) 16 L2 protein and thereby provide the opportunity for extensive in vitro analysis with respect to structural and biochemical information on L2 proteins and mechanistic details in viral entry. Read More

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http://dx.doi.org/10.1515/hsz-2018-0311DOI Listing
October 2018
3 Reads

Structural and mechanistic aspects of S-S bonds in the thioredoxin-like family of proteins.

Biol Chem 2018 Nov 20. Epub 2018 Nov 20.

UCIBIO@REQUIMTE, Departamento de Química e Bioquímica, Faculdade de Ciências, Universidade do Porto, Rua do Campo Alegre, s/n, 4169-007 Porto, Portugal.

Disulfide bonds play a critical role in a variety of structural and mechanistic processes associated with proteins inside the cells and in the extracellular environment. The thioredoxin family of proteins like thioredoxin (Trx), glutaredoxin (Grx) and protein disulfide isomerase, are involved in the formation, transfer or isomerization of disulfide bonds through a characteristic thiol-disulfide exchange reaction. Here, we review the structural and mechanistic determinants behind the thiol-disulfide exchange reactions for the different enzyme types within this family, rationalizing the known experimental data in light of the results from computational studies. Read More

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http://www.degruyter.com/view/j/bchm.just-accepted/hsz-2018-
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http://dx.doi.org/10.1515/hsz-2018-0319DOI Listing
November 2018
10 Reads

Dynamic characteristics of the mitochondrial genome in SCNT pigs.

Biol Chem 2018 Nov 13. Epub 2018 Nov 13.

College of Animal Science and Technology, China Agricultural University, 100193 Beijing, China.

Most animals generated by somatic cell nuclear transfer (SCNT) are heteroplasmic; inheriting mitochondrial genetics from both donor cells and recipient oocytes. However, the mitochondrial genome and functional mitochondrial gene expression in SCNT animals are rarely studied. Here, we report the production of SCNT pigs to study introduction, segregation, persistence and heritability of mitochondrial DNA transfer during the SCNT process. Read More

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http://dx.doi.org/10.1515/hsz-2018-0273DOI Listing
November 2018
1 Read
3.268 Impact Factor

Characterization of PdCP1, a serine carboxypeptidase from Pseudogymnoascus destructans, the causal agent of White-nose Syndrome.

Biol Chem 2018 Nov;399(12):1375-1388

Department of Molecular Microbiology and Immunology, Brown University, 171 Meeting Street, Providence, RI 02912, USA.

Pseudogymnoascus destructans is a pathogenic fungus responsible for White-nose Syndrome (WNS), a disease afflicting multiple species of North American bats. Pseudogymnoascus destructans infects susceptible bats during hibernation, invading dermal tissue and causing extensive tissue damage. In contrast, other Pseudogymnoascus species are non-pathogenic and cross-species comparisons may therefore reveal factors that contribute to virulence. Read More

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http://www.degruyter.com/view/j/bchm.ahead-of-print/hsz-2018
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http://dx.doi.org/10.1515/hsz-2018-0240DOI Listing
November 2018
16 Reads

Highlight: Frontiers in Proteolysis.

Biol Chem 2018 Nov;399(12):1351

Department of Biochemistry and Molecular Biology, University of British Columbia, Vancouver, British Columbia, Canada V6T 1Z3.

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http://dx.doi.org/10.1515/hsz-2018-0401DOI Listing
November 2018
1 Read

The structural biology of the shelterin complex.

Authors:
Yong Chen

Biol Chem 2018 Nov 8. Epub 2018 Nov 8.

State Key Laboratory of Molecular Biology, National Center for Protein Science Shanghai, Shanghai Science Research Center, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, University of Chinese Academy of Sciences, 333 Haike Road, Shanghai 201210, China.

The shelterin complex protects telomeric DNA and plays critical roles in maintaining chromosome stability. The structures and functions of the shelterin complex have been extensively explored in the past decades. This review summarizes the current progress on structural studies of shelterin complexes from different species. Read More

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http://dx.doi.org/10.1515/hsz-2018-0368DOI Listing
November 2018
1 Read

Oxidative stress and antioxidants in the pathophysiology of malignant melanoma.

Biol Chem 2018 Oct 31. Epub 2018 Oct 31.

Department of Phisiology, University of Valencia, 46010 Valencia, Spain.

The high number of somatic mutations in the melanoma genome associated with cumulative ultra violet (UV) exposure has rendered it one of the most difficult of cancers to treat. With new treatment approaches based on targeted and immune therapies, drug resistance has appeared as a consistent problem. Redox biology, including reactive oxygen and nitrogen species (ROS and RNS), plays a central role in all aspects of melanoma pathophysiology, from initiation to progression and to metastatic cells. Read More

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http://dx.doi.org/10.1515/hsz-2018-0327DOI Listing
October 2018
1 Read

Evidence for a protective role of CX3CL1/CX3CR1 axis in a model of amyotrophic lateral sclerosis.

Biol Chem 2018 Oct 1. Epub 2018 Oct 1.

Department of Neurology, The Second Hospital of Hebei Medical University, Shijiazhuang 050000, Hebei, People's Republic ofChina.

Aberrant microglial activation and Neuroinflammation is a pathological hallmark of amyotrophic lateral sclerosis (ALS). Fractalkine (CX3CL1) is almost expressed on neuronal cells. The fractalkine receptor (CX3CR1) is predominantly expressed on microglia. Read More

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http://dx.doi.org/10.1515/hsz-2018-0204DOI Listing
October 2018
3 Reads

miR-34a-5p aggravates hypoxia-induced apoptosis by targeting ZEB1 in cardiomyocytes.

Biol Chem 2019 Jan;400(2):227-236

Department of Cardiology, China-Japan Union Hospital of Jilin University, Jilin Key Laboratory for Gene Diagnosis of Cardiovascular Disease, Jilin Engineering Laboratory for Endothelial Function and Genetic Diagnosis, No. 126, Xiantai Street, Changchun, Jilin 130033, China.

Myocardial infarction (MI) is an unsolved health problem which seriously affects human health around the world. miR-34a-5p acting as a tumor-suppressor is associated with left ventricular remodeling. We aimed to explore the functional roles of miR-34a-5p in cardiomyocytes. Read More

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http://dx.doi.org/10.1515/hsz-2018-0195DOI Listing
January 2019
1 Read

Formylglycine-generating enzymes for site-specific bioconjugation.

Biol Chem 2019 Feb;400(3):289-297

Organic and Bioorganic Chemistry, Faculty of Chemistry, Bielefeld University, Universitätsstraße 25, D-33615 Bielefeld, Germany.

Site-specific bioconjugation strategies offer many possibilities for directed protein modifications. Among the various enzyme-based conjugation protocols, formylglycine-generating enzymes allow to posttranslationally introduce the amino acid Cα-formylglycine (FGly) into recombinant proteins, starting from cysteine or serine residues within distinct consensus motifs. The aldehyde-bearing FGly-residue displays orthogonal reactivity to all other natural amino acids and can, therefore, be used for site-specific labeling reactions on protein scaffolds. Read More

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http://dx.doi.org/10.1515/hsz-2018-0358DOI Listing
February 2019
2 Reads

FAM134B promotes adipogenesis by increasing vesicular activity in porcine and 3T3-L1 adipocytes.

Biol Chem 2018 Oct 23. Epub 2018 Oct 23.

College of Animal Sciences, Zhejiang University, Zhejiang, P.R. China.

Family with sequence similarity 134, Member B (FAM134B), is a cis-Golgi transmembrane protein that is known to be necessary for the long-term survival of nociceptive and autonomic ganglion neurons. Recent work has shown that FAM134B plays a pivotal role in autophagy-mediated turnover of endoplasmic reticulum (ER) membranes, tumor inhibition and lipid homeostasis. In this study, we provide mechanistic links between FAM134B and ARF-related protein 1 (ARFRP1) and further show that FAM134B resides in the Golgi apparatus. Read More

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http://dx.doi.org/10.1515/hsz-2018-0336DOI Listing
October 2018
2 Reads

Microbial transglutaminase for biotechnological and biomedical engineering.

Biol Chem 2019 Feb;400(3):257-274

Institute for Organic Chemistry and Biochemistry, Technische Universität Darmstadt, Alarich-Weiss-Straße 4, D-64287 Darmstadt, Germany.

Research on bacterial transglutaminase dates back to 1989, when the enzyme has been isolated from Streptomyces mobaraensis. Initially discovered during an extensive screening campaign to reduce costs in food manufacturing, it quickly appeared as a robust and versatile tool for biotechnological and pharmaceutical applications due to its excellent activity and simple handling. While pioneering attempts to make use of its extraordinary cross-linking ability resulted in heterogeneous polymers, currently it is applied to site-specifically ligate diverse biomolecules yielding precisely modified hybrid constructs comprising two or more components. Read More

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http://www.degruyter.com/view/j/bchm.ahead-of-print/hsz-2018
Publisher Site
http://dx.doi.org/10.1515/hsz-2018-0335DOI Listing
February 2019
9 Reads