2,956,332 results match your criteria Biological Agriculture & Horticulture[Journal]
Ecol Lett 2018 Dec 17. Epub 2018 Dec 17.
Department of Animal and Plant Sciences, University of Sheffield, Western Bank, Sheffield, S10 2TN, UK.
C photosynthesis is a complex trait that boosts productivity in warm environments. Paradoxically, it evolved independently in numerous plant lineages, despite requiring specialised leaf anatomy. The anatomical modifications underlying C evolution have previously been evaluated through interspecific comparisons, which capture numerous changes besides those needed for C functionality. Read More
J Food Sci 2018 Dec 17. Epub 2018 Dec 17.
Dept. of Microbiology and Molecular Biology, Chungnam Natl. Univ., 99 Daehak-ro, Yuseong-gu, Daejeon, 34134, Republic of Korea.
In this study, the phenolic components, as well as the antioxidant and antimicrobial activities, of the ripe and unripe fruit of pawpaw (Asimina triloba [L.] Dunal) extracted using five different solvents (distilled water, 95% methanol, 80% methanol, 95% ethanol, and 80% ethanol) were analyzed. The total phenolic content and total flavonoid content were the highest in the 95% ethanol (149. Read More
Fortschr Neurol Psychiatr 2018 Dec 17. Epub 2018 Dec 17.
Zentralinstitut für Seelische Gesundheit, AG Schizophrenieforschung; Medizinische Fakultät Mannheim / Universität Heidelberg.
The concept of mental disorder has many facets. Specific concepts of disease or disorder stand for individual entities, classified as diagnoses. Ideally, they are based on discrete physical, neurobiological or - as is the case with hereditary diseases - genetic findings. Read More
Phys Biol 2018 Dec 17. Epub 2018 Dec 17.
Department of Bioengineering, Rice University, 6100 Main Street, MS-142, Houston, TX 77251-1892, USA, Houston, Texas, UNITED STATES.
The epithelial-mesenchymal transition (EMT) plays a central role in cancer metastasis and drug resistance - two persistent clinical challenges. Epithelial cells can undergo a partial or full EMT, attaining either a hybrid epithelial/mesenchymal (E/M) or mesenchymal phenotype, respectively. Recent studies have emphasized that hybrid E/M cells may be more aggressive than their mesenchymal counterparts. Read More
Biomed Mater 2018 Dec 17. Epub 2018 Dec 17.
Shanghai Key Laboratory of Orthopedic Implant, Department of Orthopedic Surgery, Shanghai Ninth People's Hospital Affiliated Shanghai Jiao Tong University School of Medicine, Shanghai, CHINA.
Biological regeneration of articular cartilage continues to be a challenge at present. Functional engineered implants with patient-specific sizes are difficult to achieve. The aim of this study is to fabricate a biocompatible cell-laden hydrogel with a designable structure. Read More
Aging (Albany NY) 2018 Dec 16. Epub 2018 Dec 16.
Department of General Zoology, Faculty of Biology, University of Duisburg-Essen, Essen, Germany.
Many aging-associated physiological changes are known to occur in short- and long-lived species with different trajectories. Emerging evidence suggests that numerous life history trait differences between species are based on interspecies variations in gene expression. Little information is available, however, about differences in transcriptome changes during aging between mammals with diverging lifespans. Read More
Plant Physiol Biochem 2018 Dec 11;135:194-205. Epub 2018 Dec 11.
Vytautas Magnus University, Department of Environmental Sciences, Vileikos St. 8-223, LT-44404, Kaunas, Lithuania.
The initial aim of this study was to evaluate an effect of elevated CO concentration and air temperature (future climate) and O pollution on mono- and mixed-culture grown summer rape (Brassica napus L.) and wild mustard (Sinapis arvensis L.). Read More
Spectrochim Acta A Mol Biomol Spectrosc 2018 Dec 11;211:272-279. Epub 2018 Dec 11.
Key Laboratory of Chemical Biology, Molecular Engineering of Ministry of Education, Institute of Molecular Science, Shanxi University, Taiyuan 030006, China. Electronic address:
The aggregation-induced emission (AIE) luminogens are now showing strong potential in mimicking the energy donor of fluorescence resonance energy transfer (FRET) system. Herein, one highly efficient FRET system 1-NiR is successfully fabricated in aqueous solution based on an AIE active compound 1 and fluorescence dyes (Nile red (NiR)). 1 acts as the energy donor and NiR acts as the acceptor in the FRET system with the optimum concentrations ratio /[NiR] = 100. Read More
Cell Calcium 2018 Dec 11;77:68-76. Epub 2018 Dec 11.
Institute of Biology and Molecular Genetics (IBGM), Department of Biochemistry and Molecular Biology and Physiology, Faculty of Medicine, University of Valladolid and CSIC, Ramón y Cajal, 7, E-47005 Valladolid, Spain. Electronic address:
The 5' AMP-activated protein kinase (AMPK) is a nutrient-sensitive kinase that plays a key role in the control of cellular energy metabolism. We have explored here the relationship between AMPK and Ca signaling by looking at the effect of an AMPK activator (A769662) and an AMPK inhibitor (dorsomorphin) on histamine-induced Ca-release from the endoplasmic reticulum (ER) in HeLa cells. Our data show that incubation with A769662 (EC = 29 μM) inhibited histamine-induced Ca-release from the ER in intact cells, as well as inositol-1,4,5-trisphosphate (IP)-induced Ca release in permeabilized cells. Read More
Biosens Bioelectron 2018 Dec 7;126:792-799. Epub 2018 Dec 7.
DBT-National Institute of Animal Biotechnology (DBT-NIAB), Hyderabad 500032, Telangana, India. Electronic address:
Human immunodeficiency virus (HIV), which isa worldwide public health issue, is commonly associated with cardiovascular disorders (CVDs) and rheumatoid arthritis (RA). A smart nanosensor was developed for the detection of HIV and its related diseases (CVDs and RA) using graphene-based field-effect transistors (FETs). In this study, amine-functionalized graphene (afG) was conjugated with antibodies [anti-p24 for HIV, anti-cardiac troponin 1 (anti-cTn1) for CVDs, and anti-cyclic citrullinated peptide (anti-CCP) for RA] to detect various biomarkers. Read More
Eur J Med Chem 2018 Dec 4;163:583-596. Epub 2018 Dec 4.
State Key Laboratory of Natural and Biomimetic Drugs, Department of Chemical Biology, School of Pharmaceutical Sciences, Peking University, No. 38 Xueyuan Road, 100191, Haidian District, Beijing, China. Electronic address:
Sulfated hyaluronic acid (sHA) is chemically synthetic mimetic of glycosaminoglycan (GAG) presenting promising biological functions. Specific sulfation pattern, termed as sulfation code plays critical roles in regulating the binding mode between GAG and proteins. As a structural analogue of chondroitin sulfate (CS), sHA bears much higher molecular weight and is nearly free of other proteoglycan contaminants. Read More
Bioresour Technol 2018 Dec 8;274:525-532. Epub 2018 Dec 8.
Department of Housing and Urban-Rural Development of Hunan Provincial, Changsha 410082, PR China.
This study explored the cooperative effects of hydrothermal or activators pretreatment and pyrolysis process on the chemical composition of bean dreg (BD)-derived carbon materials and its adsorption characteristics for methylene blue (MB) further. The results showed that hydrothermal assisted pyrolysis carbon with or without activator (HBK and HB) possess higher BET surface area than no hydrothermal assisted carbon (BKs and B, respectively). But surface area alone was not a critical factor contributing to MB adsorption, it also depends on strong electrostatic interaction and the hydrogen bonding interaction between N in MB combines with OH and COO group of adsorbent surface by pH-dependent adsorption and pseudo-second-order model. Read More
Forensic Sci Int 2018 Dec 6;295:36-45. Epub 2018 Dec 6.
Jagiellonian University, Faculty of Chemistry, Gronostajowa 2, 30-387 Kraków, Poland.
Introduction: Although there is an observed decline in the number of new psychoactive substances entering the drug market, this phenomenon is still a significant public health problem. Synthetic cathinones are the second largest group of new substances and currently belong to the most frequently confiscated. Their availability and prevalence cause that they are often abused. Read More
Comput Biol Chem 2018 Dec 7;78:190-204. Epub 2018 Dec 7.
Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics (Theranostics), School of Pharmacy, Tianjin Medical University, Tianjin 300070, China. Electronic address:
Background: Phosphoinositide-3-kinase Delta (PI3Kδ) plays a key role in B-cell signal transduction and inhibition of PI3Kδ is confirmed to have clinical benefit in certain types of activation of B-cell malignancies. Virtual screening techniques have been used to discover new molecules for developing novel PI3Kδ inhibitors with little side effects.
Method: Computer aided drug design method were used to rapidly screen optimal PI3Kδ inhibitors from the Asinex database. Read More
Environ Pollut 2018 Dec 7;246:237-248. Epub 2018 Dec 7.
Behavioural Ecology and Ecophysiology Group, Department of Biology, University of Antwerp, Universiteitsplein 1, 2610, Wilrijk, Belgium. Electronic address:
Over the past decades, there has been growing scientific attention and public concern towards perfluoroalkyl acids (PFAAs), due to their widespread presence in the environment and associations with adverse effects on various organisms. Bird eggs have often been used as less-invasive biomonitoring tools for toxicological risk assessments of persistent organic pollutants, including some PFAAs. Hereby, it is typically assumed that one random egg is representative for the PFAA concentrations of the whole clutch. Read More
J Photochem Photobiol B 2018 Nov 22;191:13-17. Epub 2018 Nov 22.
Department of pharmacy, Fujian Provincial Hospital& Fujian Medical University, People's Republic of China; Molecular Biology Laboratory of Chinese Traditional Medicine, Fujian Provincial Hospital, People's Republic of China. Electronic address:
Multifunctional nanocarriers are in immediate need to develop anticancer activity for the treatment of cancers. In the present research, the graphene oxide was reduced via an efficient method which reduced to RGO by using Euphorbia milii leaves extract. Thus obtained RGO nanocomposites were subsequently characterized by means UV-Vis absorption technique. Read More
iScience 2018 Dec 5;10:234-244. Epub 2018 Dec 5.
Centre for Developmental Neurobiology, Institute of Psychiatry, Psychology and Neuroscience, King's College London, London SE1 1UL, UK; MRC Centre for Neurodevelopmental Disorders, King's College London, New Hunt's House, Guy's Campus, London SE1 1UL, UK. Electronic address:
Directed transport of transmembrane proteins is generally believed to occur via intracellular transport vesicles. However, using single-particle tracking in rat hippocampal neurons with a pH-sensitive quantum dot probe that specifically reports surface movement of receptors, we have identified a subpopulation of neuronal EphB2 receptors that exhibit directed motion between synapses within the plasma membrane itself. This receptor movement occurs independently of the cytoskeleton but is dependent on cholesterol and is regulated by neuronal activity. Read More
Psychoneuroendocrinology 2018 Dec 7;102:139-148. Epub 2018 Dec 7.
Cousins Center for Psychoneuroimmunology, Semel Institute for Neuroscience and Human Behavior, University of California, 300 Medical Plaza, Los Angeles, CA 90095, USA; Department of Psychology, University of California, 502 Portola Plaza, Los Angeles, CA 90095, USA. Electronic address:
Previous research has linked exposure to adverse social conditions with DNA damage and accelerated telomere shortening, raising the possibility that chronic stress may impact biological aging pathways, ultimately increasing risk for age-related diseases. Less clear, however, is whether these stress-related effects extend to additional hallmarks of biological aging, including cellular senescence, a stable state of cell cycle arrest. The present study aimed to investigate associations between psychosocial stress and two markers of cellular aging-leukocyte telomere length (LTL) and cellular senescence signal p16. Read More
Cortex 2018 Nov 17;113:1-14. Epub 2018 Nov 17.
Neuroscience and Aphasia Research Unit, Division of Neuroscience and Experimental Psychology, School of Biological Sciences, University of Manchester, UK; MRC Cognition and Brain Sciences Unit, University of Cambridge, UK. Electronic address:
In neurosurgery there are several situations that require transgression of the temporal cortex. For example, a subset of patients with temporal lobe epilepsy require surgical resection (most typically, en-bloc anterior temporal lobectomy). This procedure is the gold standard to alleviate seizures but is associated with chronic cognitive deficits. Read More
Aquat Toxicol 2018 Dec 11;207:101-109. Epub 2018 Dec 11.
Key Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Provincial Key Laboratory of Applied Marine Biology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, 510301, China. Electronic address:
Phytochelatin (PC) synthesis is thought to be a rapid and specific response to metal exposure in marine phytoplankton, but its potential as a predictor of metal toxicity is far from conclusive. Thus this research examines the bioaccumulation, PC induction, and toxicity of Cadmium (Cd) in Thalassiosira weissflogii, a coastal diatom under varying nutrient conditions. Nitrogen limitation strongly inhibited Cd uptake and PC induction at the same [Cd] level, and increased metal sensitivity. Read More
Chemosphere 2018 Dec 4;219:730-739. Epub 2018 Dec 4.
State Key Laboratory of Pollution Control and Resource Reuse, Center for Hydrosciences Research, School of the Environment, Nanjing University, Nanjing, PR China.
In this work, the partition of endocrine-disrupting chemicals (EDCs) in colloid-bound and truly dissolved phases in municipal wastewater before and after advanced treatment processes was determined. The effluents, which were filtered using a 0.45 μm membrane, were further separated with the 1 kDa cross-flow ultrafiltration system into two phases, namely, colloidal phase (0. Read More
Chemosphere 2018 Dec 6;219:695-703. Epub 2018 Dec 6.
UNESCO UNITWIN/WiCop Department of Physical-Chemistry, University of Cádiz, Campus Río de San Pedro, 11510, Puerto Real, Cádiz, Spain.
A passive treatment plant, located in the Iberian Pyrite Belt (Huelva, Southwest Spain), was designed for acid mine drainage remediation. Since its installation, the improvement of water quality in terms of hydrochemical composition has been demonstrated successfully. However, according to the Water Framework Directive, the treated effluent must have ecological values for potential living. Read More
Chemosphere 2018 Nov 28;219:655-661. Epub 2018 Nov 28.
Key Laboratory of Environment and Health, Ministry of Education & Ministry of Protection, and State Key Laboratory of Environmental Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China. Electronic address:
Benzophenones (BPs) are widely used as ultraviolet absorbers and fragrance retention agents. Evidences from animal studies have suggested that exposure to BPs may affect fetal growth, but human data is limited and no study is concerning critical windows of BPs exposure throughout pregnancy in relation to fetal growth. We aimed to investigate the associations of prenatal exposure to BPs with birth size and examine the critical exposure windows of fetus development. Read More
Ecotoxicol Environ Saf 2018 Dec 14;170:521-529. Epub 2018 Dec 14.
Department of Biology, Texas Christian University, Fort Worth, TX, USA. Electronic address:
Fish embryos are increasingly being utilized in aquatic toxicity testing, as evidenced by the Organisation for Economic Co-operation and Development's approval of the fish embryo acute toxicity (FET) test. However, the FET test only allows for the estimation of acute toxicity, whereas other test methods such as the larval growth and survival (LGS) test allow for the estimation of both acute and chronic toxicity. Additionally, it has been demonstrated that the FET test is less sensitive than other test methods for some neurotoxic compounds. Read More
Ecotoxicol Environ Saf 2018 Dec 14;170:495-501. Epub 2018 Dec 14.
State Key Laboratory of Environmental and Biological Analysis, Department of Chemistry, Hong Kong Baptist University, Hong Kong, China. Electronic address:
Halogenated bisphenol A analogues (X-BPA) have been widely used in industrial production, such as flame retardant. Although BPA exposure was found to result in cytotoxicity, toxicity of X-BPA and molecular mechanism remain under-explored. In this study, we employed human breast cancer cell as a model to investigate the concentration-dependent toxicity and underlying mechanisms of tetrabromo bisphenol A (TBBPA) and tetrachloro bisphenol A (TCBPA). Read More
Cell Mol Gastroenterol Hepatol 2018 Dec 14. Epub 2018 Dec 14.
Cardiovascular Research Center and Department of Cellular and Molecular Physiology, Yale University School of Medicine, New Haven, CT 06510-3221, USA; INSERM U970, Paris Cardiovascular Research Center, 75015 Paris, France.
The lymphatic system of the gut plays important roles in the transport of dietary lipids, as well as in immunosurveillance and removal of interstitial fluid. Historically, despite its crucial functions in intestinal homeostasis, the lymphatic system has been poorly studied. In the last two decades, identification of specific molecular mediators of lymphatic endothelial cells (LECs) growth together with novel genetic approaches and intravital imaging techniques, have advanced our understanding of the mechanisms regulating intestinal lymphatic physiology in health and disease. Read More
Biochim Biophys Acta Mol Basis Dis 2018 Dec 14. Epub 2018 Dec 14.
Department of Functional Genomics, Interfaculty Institute of Genetics and Functional Genomics, University Medicine Greifswald, Felix-Hausdorff-Strasse 8, 17489 Greifswald, Germany.
Mutations in the X chromosomal tRNA 2'‑O‑methyltransferase FTSJ1 cause intellectual disability (ID). Although the gene is ubiquitously expressed affected individuals present no consistent clinical features beyond ID. In order to study the pathological mechanism involved in the aetiology of FTSJ1 deficiency-related cognitive impairment, we generated and characterized an Ftsj1 deficient mouse line based on the gene trapped stem cell line RRD143. Read More
Int J Radiat Oncol Biol Phys 2018 Dec 14. Epub 2018 Dec 14.
Centre for Cancer Research & Cell Biology, Queen's University Belfast, 97 Lisburn Road, Belfast BT9 7AE, United Kingdom.
Introduction: Despite the effectiveness of RaCl for treating patients with symptomatic bone metastatic disease, its mechanisms of action are still unclear. Even established dosimetric approaches differ considerably in their conclusions. In silico tumour models bring a new perspective to this as they can quantitatively simulate the interaction of α-particles with the target(s). Read More
Food Chem Toxicol 2018 Dec 14. Epub 2018 Dec 14.
CNC - Center for Neuroscience and Cell Biology, University of Coimbra, UC Biotech Building, Biocant Park, 3060-197, Cantanhede, Portugal. Electronic address:
Dose-dependent and cumulative cardiotoxicity associated with doxorubicin (DOX) is the main limitation of anticancer therapy. Pediatric cancer survivors are particularly vulnerable, and no effective prevention measures are available. The aim of the present study was to investigate the persistent effects of nanomolar DOX concentrations and determine whether a pretreatment would induce mitochondrial adaptations in H9c2 cardiomyoblasts. Read More
J Proteomics 2018 Dec 14. Epub 2018 Dec 14.
Department of Biology, Hong Kong Baptist University, Hong Kong, China; HKBU Institute of Research and Continuing Education, Shenzhen, China. Electronic address:
Most gastropods deposit their eggs either on land or underwater, but the family Ampullariidae includes members that exhibit both underwater and aerial oviposition, making it an ideal model for understanding mechanisms underlying the evolutionary transition from water to land. We applied SDS-PAGE and LC-MS/MS to analyse the proteome of the egg perivitelline fluid (PVF) of Marisa cornuarietis - an aquatic ovipositing ampullariid. Comparison with the reported PVF proteomes of two aerial ovipositing ampullariids (Pomacea canaliculata and P. Read More
J Proteomics 2018 Dec 14. Epub 2018 Dec 14.
Center for Advanced Proteomics Research and Department of Microbiology, Biochemistry and Molecular Genetics, Rutgers University - New Jersey Medical School and Cancer Institute of New Jersey, Newark, NJ 07103, USA. Electronic address:
Disulfide bonds (SS) are post-translational modifications important for the proper folding and stabilization of many cellular proteins with therapeutic uses, including antibodies and other biologics. With budding advances of biologics and biosimilars, there is a mounting need for a robust method for accurate identification of SS. Even though several mass spectrometry methods have emerged for this task, their practical use rests on the broad effectiveness of both sample preparation methods and bioinformatics tools. Read More
J Proteomics 2018 Dec 14. Epub 2018 Dec 14.
Comprehensive Pneumology Center (CPC), University Hospital, Ludwig-Maximilians University, Helmholtz Zentrum München, Member of the German Center for Lung Research (DZL), Munich, Germany. Electronic address:
Proteasome dysfunction is emerging as a novel pathomechanism for the development of chronic obstructive pulmonary disease (COPD), a major leading cause of death in the world. Cigarette smoke, one of the main risk factors for COPD, impairs proteasome function in vitro and in vivo. In the present study, we dissected the molecular changes induced by cigarette smoke on the proteasome in lung epithelial cells and mouse lungs. Read More
Neuroimage 2018 Dec 14. Epub 2018 Dec 14.
Laboratory of Behavioral Neuroscience, National Institute on Aging, Baltimore, MD 21224, USA.
White matter microstructure can be measured with diffusion tensor imaging (DTI). While increasing age is a predictor of white matter (WM) microstructure changes, roles of other possible modifiers, such as cardiovascular risk factors, APOE ε4 allele status and biological sex have not been clarified. We investigated 665 cognitively normal participants from the Baltimore Longitudinal Study of Aging (age 50-95, 56. Read More
Neurobiol Dis 2018 Dec 14. Epub 2018 Dec 14.
Institute of Biomedicine, University of Eastern Finland, Kuopio 70210, Finland. Electronic address:
No single-omic approach completely elucidates the multitude of alterations taking place in Alzheimer's disease (AD). Here, we coupled transcriptomic and phosphoproteomic approaches to determine the temporal sequence of changes in mRNA, protein, and phosphopeptide expression levels from human temporal cortical samples, with varying degree of AD-related pathology. This approach highlighted fluctuation in synaptic and mitochondrial function as the earliest pathological events in brain samples with AD-related pathology. Read More
Neuroscience 2018 Dec 14. Epub 2018 Dec 14.
Division of Infectious Diseases, University of Colorado Anschutz Medical Campus, B168 12700 E. 19th Avenue, Aurora, CO 80045, USA. Electronic address:
The central nervous system (CNS) and gastrointestinal tract (GIT) are linked through neuro-endocrine and humoral pathways. Critically ill patients suffer severe physical and emotional stress and frequently receive acid suppressants; however, stress and acid suppression may alter GIT microbiota. This study evaluated the effects of acid suppression on the GIT microbiota and genome-wide expression of brain-specific genes in a murine model of restraint stress. Read More
Int J Biol Macromol 2018 Dec 14. Epub 2018 Dec 14.
Malaysian Institute of Pharmaceuticals and Nutraceuticals, NIBM, 11700 Gelugor, Penang, Malaysia; School of Biological Sciences, Universiti Sains Malaysia, 11800 Minden, Penang, Malaysia; Centre for Chemical Biology, Universiti Sains Malaysia, 11900 Bayan Lepas, Penang, Malaysia. Electronic address:
P(3HB-co-4HB) with a high 4HB monomer composition was previously successfully produced using the transformant Cupriavidus malaysiensis USMAA1020 containing an additional copy of the PHA synthase gene. In this study, high PHA density fed-batch cultivation strategies were developed for such 4HB-rich P(3HB-co-4HB). The pulse, constant and mixed feeding strategies resulted in high PHA accumulation, with a PHA content of 74-92 wt% and 4HB monomer composition of 92-99 mol%. Read More
Pharmacol Ther 2018 Dec 14. Epub 2018 Dec 14.
Aix Marseille Université, INSERM, INRA, C2VN, Marseille, France; Clinique des Bronches, Allergies et Sommeil, Hôpital Nord, AP-, HM, Marseille, France.. Electronic address:
KIT is a receptor tyrosine kinase that after binding to its ligand stem cell factor activates signaling cascades linked to biological processes such as proliferation, differentiation, migration and cell survival. Based on studies performed on SCF and/or KIT mutant animals that presented anemia, sterility, and/or pigmentation disorders, KIT signaling was mainly considered to be involved in the regulation of hematopoiesis, gametogenesis, and melanogenesis. More recently, novel animal models and ameliorated cellular and molecular techniques have led to the discovery of a widen repertoire of tissue compartments and functions that are being modulated by KIT. Read More
Int J Biochem Cell Biol 2018 Dec 14. Epub 2018 Dec 14.
Laboratory for Cancer Biology, Department of Medical Oncology and Clinical Research, Cancer Institute (WIA), Chennai, India. Electronic address:
Induced pluripotent stem cells are derived from adult somatic cells by ectopic expression of stem cell factors OCT4, SOX2, MYC and KLF4. These cells have characteristic features similar to embryonic stem cells. Although there exists in vitro and in vivo models of cancer, recapitulating the earliest events in the pathogenesis remain challenging. Read More
Fungal Genet Biol 2018 Dec 14. Epub 2018 Dec 14.
Department of Biology, University of New Mexico, Albuquerque, New Mexico 87131 USA. Electronic address:
A survey of genomes reported here for 10 isolates of Monosporascus species and an additional 25 genomes from other members of the Xylariales (representing 15 genera) available in public databases indicated that genes typically associated with MAT1-1 (mat A) or MAT1-2 (mat a) mating types are absent or have diverged greatly relative to counterparts in other Pezizomycotina. This was particularly surprising for isolates known to be homothallic, given that homothallic members of the Pezizomycotina typically possess a MAT1-1-1 (mat A-1) gene and one or both of two other closely-linked mating-type genes, MAT1-1-2 (mat A-2) and MAT1-1-3 (mat A-3), in addition to MAT1-2-1 (mat a-1). We failed to detect candidate genes for either MAT1-1-1 or MAT1-1-2 in any member of the Xylariales. Read More
Comp Biochem Physiol B Biochem Mol Biol 2018 Dec 14. Epub 2018 Dec 14.
Department of Biology, PO Box 5000, Shepherd University, Shepherdstown, WV. USA. 25443.
Determination of how carotenoid-based color patches change with pigmentary access is important to understanding color patch function in animals. We performed a carotenoid addition experiment on male painted turtles (Chrysemys picta) to determine if an increase in dietary carotenoids led to location-specific changes in painted turtle spot and stripe color. Turtles fed large quantities of carotenoids had increased yellow chroma in chin stripes and increased red chroma in the neck and carapace stripes, as well as reduced ultraviolet (UV) chroma in the neck (orange to the human eye) and fore -limb (red to the human eye) stripes. Read More
Protein Expr Purif 2018 Dec 14. Epub 2018 Dec 14.
School of Biology and Biological Engineering, South China University of Technology, Guangzhou, Guangdong, 510006, China; Guangdong Provincial Key Laboratory of Fermentation and Enzyme Engineering, Guangzhou, 510006, China. Electronic address:
Nuclease P1 has been used for the industrial production of 5'-monouncleotides from yeast RNA due to it's single-strand specific nucleases activity. The high demand for 5'-nucleotides in the manufacture of food and drugs requires the commercial scale production of nuclease P1, but the fermentation level of nuclease P1 from Penicillium citrinum still remains low. In this study, the Penicillium citrinum nuclease P1 gene (nuc P1) was heterologously expressed in A. Read More
Eur J Pharm Sci 2018 Dec 14. Epub 2018 Dec 14.
School of Pharmaceutical Sciences, Zhengzhou University, No.100. Kexue Road, Zhengzhou, Henan Province 450001, China; Key Laboratory of Targeting Therapy and Diagnosis for Critical Diseases, Zhengzhou 450001, China; Collaborative Innovation Center of New Drug Research and Safety Evaluation, Henan Province, China. Electronic address:
We recently reported that the absolute oral bioavailability of flavonoid Morin was extremely low at 0.45%, resulting in unsatisfied therapeutic efficacy in vivo. The present study was aimed to systemically assess the pre-absorption risks of Morin for rationale formulation design. Read More
Cell Signal 2018 Dec 14. Epub 2018 Dec 14.
Department of Pathology, Shantou Central Hospital, Affiliated Shantou Hospital of Sun Yat-sen University, Shantou 515041, China; Guangdong Province Key Laboratory of Malignant Tumor Epigenetics and Genes Regulation, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou 510120, China. Electronic address:
High level expression of lipocalin 2 (LCN2) usually indicates poor prognosis in esophageal squamous cell carcinoma (ESCC) and many other cancers. Our previous study showed LCN2 promotes migration and invasion of ESCC cells through a novel positive feedback loop. However, the key transcription activation protein (KTAP) in the loop had not yet been identified. Read More
Biosystems 2018 Dec 14. Epub 2018 Dec 14.
School of Mathematical and Computer Sciences, Heriot-Watt University, UK.
How complex traits arise within organisms over evolutionary time is an important question that has relevance both to the understanding of biological systems and to the design of bio-inspired computing systems. This paper investigates the process of acquiring complex traits within epiNet, a recurrent connectionist architecture capable of adapting its topology during execution. Inspired by the biological processes of gene regulation and epigenetics, epiNet captures biological organisms' ability to alter their regulatory topologies according to environmental stimulus. Read More
Biochimie 2018 Dec 14. Epub 2018 Dec 14.
Faculdade São Leopoldo Mandic, Instituto de Pesquisas São Leopoldo Mandic, Área de Imunologia, Campinas, Brazil. Electronic address:
Inflammation of temporomandibular joint (TMJ) tissues are the most common cause of pain conditions associated with temporomandibular disorders (TMDs). After a tissue and/or neural damage, the inflammatory response is characterized by plasma extravasation and leukocytes infiltration in the TMJ tissues, which in turn, release inflammatory cytokines cascades responsible for inflammatory pain. Lectins are glycoproteins widely distributed in nature that may exhibit anti-inflammatory properties. Read More
Virus Res 2018 Dec 14. Epub 2018 Dec 14.
State Key Laboratory of Agrobiotechnology, Department of Biochemistry and Molecular Biology, College of Biological Sciences, China Agricultural University, Beijing 100193, PR China. Electronic address:
We have isolated 4 naturally-occurring strains of CPV in mainland China and have identified them as CPV-2, 2a, 2b and 2c genotypes according to their VP2 sequences which also revealed substitutions within their right terminal regions. To determine if these substitutions affected the growth characteristics of the 4 strains, we constructed plasmids based on their genomic sequences minus their right terminal sequences, with the latter replaced by a single right terminal region. Analysis of rescued recombinants showed that the substitutions within their natural right termini had no significant effect on their growth characteristics. Read More
Exp Eye Res 2018 Dec 14. Epub 2018 Dec 14.
Department of Biology, Faculty of Science, University of A Coruña, Spain. Electronic address:
The aim off the present study was to evaluate the effect and the mechanism of action of the conditioned medium from human uterine cervical stem cells (CM-hUCESC) on corneal wound healing in a rabbit dry eye model. To do this, dry eye and corneal epithelial injuries were induced in rabbits by topical administration of atropine sulfate and NaOH. Hematoxylin-Eosin (H&E) and Ki-67 immunostaining were carried out to evaluate corneal damage and cell proliferation, and real-time PCR was used to evaluate proinflammatory cytokines in the cornea. Read More
Exp Eye Res 2018 Dec 14. Epub 2018 Dec 14.
Institute of Eye Research, Buddhist Tzu Chi General Hospital, Hualien, Taiwan; Institute of Medical Sciences, Tzu Chi University, Hualien, Taiwan. Electronic address:
Reproducible skills are essential for successful induction of a rat model of anterior ischemic optic neuropathy (rAION). We established an in vivo validation index by measuring the natural course of optic nerve head (ONH) width and retinal nerve fiber layer (RNFL) thickness in the rAION model using optical coherence tomography (OCT). The rAION model was induced by photodynamic operations. Read More
Math Biosci 2018 Dec 14. Epub 2018 Dec 14.
Department of Chemical and Biochemical Engineering, Rutgers, The State University of New Jersey, 98 Brett Road, Piscataway, NJ 08854; Department of Biomedical Engineering, Rutgers The State University of New Jersey, 599 Taylor Road, Piscataway, NJ 08854. Electronic address:
Pharmacokinetic models are particularly useful to study the underlying and complex physiological mechanisms contributing to clinical differences across patient subgroups or special populations. Unfortunately, the inherent variability of biological systems and knowledge gaps in physiological data limit confidence in model predictions for special populations. Sourcing data to reflect the desired physiologies can be resource intensive, particularly for a larger model. Read More
Chem Phys Lipids 2018 Dec 14. Epub 2018 Dec 14.
Division of Chemistry, School of Pharmacy and Biological Sciences, University of Brighton, BN2 4GL, United Kingdom. Electronic address:
Cells contain high levels of macromolecular crowding; understanding how macromolecular crowding impacts the behaviour of biological systems can give new insights into biological phenomena and disease pathologies. In this study, we assess the effect of macromolecular crowding on the catalytic activity of the biomembrane binding protein phospholipase A (PLA). Using 3D-printed equilibrium dialysis chambers we show that macromolecular crowding increases the binding of PLA to lipid vesicles. Read More