89,016 results match your criteria Biologia Plantarum[Journal]


Merulinic acid C overcomes gentamicin resistance in Enterococcus faecium.

Bioorg Chem 2020 May 12;100:103921. Epub 2020 May 12.

Brazilian Biosciences National Laboratory (LNBio), CNPEM, 13083-970 Campinas, SP, Brazil; Univ. Grenoble Alpes, CNRS, CEA, Institut de Biologie Structurale (IBS), Grenoble, France. Electronic address:

Enterococci are gram-positive, widespread nosocomial pathogens that in recent years have developed resistance to various commonly employed antibiotics. Since finding new infection-control agents based on secondary metabolites from organisms has proved successful for decades, natural products are potentially useful sources of compounds with activity against enterococci. Herein are reported the results of a natural product library screening based on a whole-cell assay against a gram-positive model organism, which led to the isolation of a series of anacardic acids identified by analysis of their spectroscopic data and by chemical derivatizations. Read More

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http://dx.doi.org/10.1016/j.bioorg.2020.103921DOI Listing

Decellularized Tissues as Platforms for In Vitro Modeling of Healthy and Diseased Tissues.

Acta Biomater 2020 May 25. Epub 2020 May 25.

J. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, 1275 Center Dr. BMS J257, Gainesville, FL USA 32611. Electronic address:

Biomedical engineers are at the forefront of developing novel treatments to improve human health, however, many products fail to translate to clinical implementation. In vivo pre-clinical animal models, although the current best approximation of complex disease conditions, are limited by reproducibility, ethical concerns, and poor accurate prediction of human response. Hence, there is a need to develop physiologically relevant, low cost, scalable, and reproducible in vitro platforms to provide reliable means for testing drugs, biomaterials, and tissue engineered products for successful clinical translation. Read More

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http://dx.doi.org/10.1016/j.actbio.2020.05.031DOI Listing

Organs infected with Trypanosoma cruzi and DTU identification in the naturally infected rodent Octodon degus.

Exp Parasitol 2020 May 25:107931. Epub 2020 May 25.

Programa de Biología Celular y Molecular, ICBM, Facultad de Medicina, Universidad de Chile, Santiago, Chile. Electronic address:

Chagas disease is a public health problem in America. Its parasite, Trypanosoma cruzi, presents different discrete typing units (DTUs), colonizes organs of mammalian hosts in chronic infections, and presents tropism for particular organs in experimental infections. We evaluated T. Read More

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http://dx.doi.org/10.1016/j.exppara.2020.107931DOI Listing

P-selectin blockade in COVID-19-related ARDS.

Am J Physiol Lung Cell Mol Physiol 2020 Jun;318(6):L1237-L1238

Centro Dipartimentale di Biologia Cellulare Cardiorespiratoria, Dipartimento di Patologia Chirurgica, Medica, Molecolare e dell'Area Critica and Azienda Ospedaliero-Universitaria Pisana, Pisa, Italy.

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http://dx.doi.org/10.1152/ajplung.00202.2020DOI Listing

Cryptic haploid stages in the life cycle of Leathesia marina (Chordariaceae, Phaeophyceae) under in vitro culture.

J Phycol 2020 May 28. Epub 2020 May 28.

CONICET-Bahía Blanca, Instituto Argentino de Oceanografía (IADO), Camino Carrindanga 7.5 km, B8000FWB, Bahía Blanca, Argentina.

We evaluated the life cycle of Leathesia marina through molecular analyses, culture studies, morphological observations and ploidy measurements. Macroscopic sporophytes were collected from two localities in Atlantic Patagonia and were cultured under long day (LD) and short day (SD) conditions. Molecular identification of the microscopic and macroscopic phases was performed through the cox3 and rbcL genes and the phylogeny was assessed on the basis of single gene and concatenated data sets. Read More

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http://dx.doi.org/10.1111/jpy.13034DOI Listing

Highlights of the 12th International Bordetella Symposium.

Clin Infect Dis 2020 May 28. Epub 2020 May 28.

Laboratory of Vaccinology and Mucosal Immunity, Université Libre de Bruxelles (U.L.B.), Brussels, Belgium.

To commemorate the 100th anniversary of the Nobel prize being awarded to Jules Bordet, the discoverer of Bordetella pertussis, the 12th International Bordetella Symposium was held from April 9-12, 2019 at the Université Libre de Bruxelles, where Jules Bordet studied and was Professor of Microbiology. The symposium attracted more than 300 Bordetella experts from 34 countries. They discussed the latest epidemiologic data and clinical aspects of pertussis, Bordetella biology and pathogenesis, immunology and vaccine development, and genomics and evolution. Read More

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http://dx.doi.org/10.1093/cid/ciaa651DOI Listing

Effect of El Niño Southern Oscillation cycle on the potential distribution of cutaneous leishmaniasis vector species in Colombia.

PLoS Negl Trop Dis 2020 May 28;14(5):e0008324. Epub 2020 May 28.

Grupo de Investigación Salud-Comunid-UDES, Programa de Medicina, Universidad de Santander, Bucaramanga, Santander, Colombia.

Local anomalies in rainfall and temperature induced by El Niño and La Niña episodes could change the structure of the vector community. We aimed to estimate the effect of the El Niño-La Niña cycle in the potential distribution of cutaneous leishmaniasis (CL) vector species in Colombia and to compare the richness of the vectors with the occurrence of CL in the state of Norte de Santander. The potential distributions of four species were modeled using a MaxEnt algorithm for the following episodes: La Niña 2010-2011, Neutral 2012-2015 and El Niño 2015-2016. Read More

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http://dx.doi.org/10.1371/journal.pntd.0008324DOI Listing

[Comparación de los perfiles de resistencia antimicrobiana de Escherichia coli uropatógena e incidencia de la producción de betalactamasas de espectro extendido en tres establecimientos privados de salud de Perú].

Biomedica 2020 May 1;40(Supl. 1):139-147. Epub 2020 May 1.

Laboratorio de Microbiología, Hospital Nacional Docente "Madre Niño San Bartolomé", Lima, Perú.

Introducción. La aparición de enterobacterias multirresistentes y productoras de betalactamasas de espectro extendido en pacientes ambulatorios con infecciones urinarias representa un problema de salud pública en Perú. Objetivo. Read More

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http://dx.doi.org/10.7705/biomedica.4772DOI Listing

[Caracterización molecular de las variantes del virus de Epstein-Barr detectadas en la cavidad oral de adolescentes de Cali, Colombia].

Biomedica 2020 May 1;40(Supl. 1):76-88. Epub 2020 May 1.

Departamento de Biología, Facultad de Ciencias Naturales y Exactas, Universidad del Valle, Cali, Colombia.

Introducción. El virus de Epstein-Barr (EBV) es un virus ubicuo y oncogénico, asociado con el desarrollo de enfermedades como la mononucleosis infecciosa, el linfoma de Burkitt, el carcinoma nasofaríngeo y otras neoplasias. Actualmente, se reconocen dos subtipos: EBV-1 y EBV- 2, que tienen diferencias genéticas con sus antígenos nucleares (Epstein-Barr Nuclear Antigens, EBNA). Read More

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http://dx.doi.org/10.7705/biomedica.4917DOI Listing

A Phosphoproteomic Analysis Pipeline for Peels of Tropical Fruits.

Methods Mol Biol 2020 ;2139:179-196

Red de Estudios Moleculares Avanzados, Clúster Científico y Tecnológico BioMimic®, Instituto de Ecología A.C. (INECOL), Veracruz, Mexico.

Phosphorylation is a posttranslational reversible modification related to signaling and regulatory mechanisms. Protein phosphorylation is linked to structural changes that modulate protein activity, interaction, or localization and therefore the cell signaling pathways. The use of techniques for phosphoprotein enrichment along with mass spectrometry has become a powerful tool for the characterization of signal transduction in model organisms. Read More

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http://dx.doi.org/10.1007/978-1-0716-0528-8_14DOI Listing
January 2020

Optimizing Shotgun Proteomics Analysis for a Confident Protein Identification and Quantitation in Orphan Plant Species: The Case of Holm Oak (Quercus ilex).

Methods Mol Biol 2020 ;2139:157-168

Agroforestry and Plant Biochemistry, Proteomics and Systems Biology, Department of Biochemistry and Molecular Biology, University of Cordoba, UCO-CeiA3, Cordoba, Spain.

The proteomics of orphan, unsequenced, and recalcitrant organisms is highly challenging. This is the case of the typical Mediterranean forest tree Holm oak (Quercus ilex). Proteomics has moved on quite fast from the classical 2DE-MS to shotgun or gel-free/label-free approaches, with the latter possessing a series of advantages over the gel-based ones. Read More

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http://dx.doi.org/10.1007/978-1-0716-0528-8_12DOI Listing
January 2020

Updating dual-specificity tyrosine-phosphorylation-regulated kinase 2 (DYRK2): molecular basis, functions and role in diseases.

Cell Mol Life Sci 2020 May 27. Epub 2020 May 27.

Instituto Maimónides de Investigación Biomédica de Córdoba (IMIBIC), Avda. Menéndez Pidal s/n., 14004, Córdoba, Spain.

Members of the dual-specificity tyrosine-regulated kinase (DYRKs) subfamily possess a distinctive capacity to phosphorylate tyrosine, serine, and threonine residues. Among the DYRK class II members, DYRK2 is considered a unique protein due to its role in disease. According to the post-transcriptional and post-translational modifications, DYRK2 expression greatly differs among human tissues. Read More

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http://dx.doi.org/10.1007/s00018-020-03556-1DOI Listing

Measured data of (Diptera Drosophilidae) development and learning and memory behaviour after copper exposition.

Data Brief 2020 Feb 13;28:104986. Epub 2019 Dec 13.

Programa de Pós-Graduação em Ciências Biológicas: Bioquímica Toxicológica, Departamento de Bioquímica e Biologia Molecular, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil.

This article presents the statistical analysis data from development (from larvae to adult) and learning and memory retention behavior of a Pavlovian conditioning in male and female flies exposed to copper. While the full data sets are available In the article: Copper decrease associative learning and memory in , this data-in-brief article includes the detailed statistical analysis performed. Data demonstrates Statistica Software analysis between the subject part of the analysis: 2 treatments x 2 sexes x 2 ages and within subject part of the analysis: 2 treatments x 2 sex x 2 ages x 4 times, repeated measures. Read More

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http://dx.doi.org/10.1016/j.dib.2019.104986DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7242787PMC
February 2020

Alphavirus Replication: The Role of Cardiac Glycosides and Ion Concentration in Host Cells.

Biomed Res Int 2020 9;2020:2813253. Epub 2020 May 9.

Departamento de Biologia Celular e Molecular, Instituto de Biologia, Universidade Federal Fluminense, Niterói, RJ CEP 24020-141, Brazil.

Alphaviruses are arthropod-borne viruses that can cause fever, rash, arthralgias, and encephalitis. The mosquito species and are the most frequent transmitters of alphaviruses. There are no effective vaccines or specific antivirals available for the treatment of alphavirus-related infections. Read More

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http://dx.doi.org/10.1155/2020/2813253DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7232666PMC

Transcriptomic changes across the life cycle of .

PeerJ 2020 14;8:e8947. Epub 2020 May 14.

Grupo de Investigaciones Microbiológicas-UR (GIMUR), Departamento de Biología, Facultad de Ciencias Naturales, Universidad del Rosario, Bogota, Colombia.

is a flagellated protozoan that causes Chagas disease; it presents a complex life cycle comprising four morphological stages: epimastigote (EP), metacyclic trypomastigote (MT), cell-derived trypomastigote (CDT) and amastigote (AM). Previous transcriptomic studies on three stages (EPs, CDTs and AMs) have demonstrated differences in gene expressions among them; however, to the best of our knowledge, no studies have reported on gene expressions in MTs. Therefore, the present study compared differentially expressed genes (DEGs), and signaling pathway reconstruction in EPs, MTs, AMs and CDTs. Read More

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http://dx.doi.org/10.7717/peerj.8947DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7231504PMC

Cell-based analysis of CAD variants identifies individuals likely to benefit from uridine therapy.

Genet Med 2020 May 28. Epub 2020 May 28.

Genome Dynamics and Function Program, Centro de Biología Molecular Severo Ochoa, CSIC-UAM, Madrid, Spain.

Purpose: Pathogenic autosomal recessive variants in CAD, encoding the multienzymatic protein initiating pyrimidine de novo biosynthesis, cause a severe inborn metabolic disorder treatable with a dietary supplement of uridine. This condition is difficult to diagnose given the large size of CAD with over 1000 missense variants and the nonspecific clinical presentation. We aimed to develop a reliable and discerning assay to assess the pathogenicity of CAD variants and to select affected individuals that might benefit from uridine therapy. Read More

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http://dx.doi.org/10.1038/s41436-020-0833-2DOI Listing

Author Correction: Challenges in the serological evaluation of dogs clinically suspect for canine leishmaniasis.

Sci Rep 2020 May 28;10(1):8988. Epub 2020 May 28.

Instituto de Investigação e Inovação em Saúde, Universidade do Porto, R. Alfredo Allen, 4200-135, Porto, Portugal.

An amendment to this paper has been published and can be accessed via a link at the top of the paper. Read More

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http://dx.doi.org/10.1038/s41598-020-66088-5DOI Listing

Adenine DNA methylation, 3D genome organization, and gene expression in the parasite .

Proc Natl Acad Sci U S A 2020 May 27. Epub 2020 May 27.

Laboratorio de Parásitos Anaerobios, Instituto Tecnológico Chascomús, National Scientific and Technical Research Council-National University of San Martin (CONICET-UNSAM), B7130IWA Chascomús, Argentina;

is a common sexually transmitted parasite that colonizes the human urogenital tract causing infections that range from asymptomatic to highly inflammatory. Recent works have highlighted the importance of histone modifications in the regulation of transcription and parasite pathogenesis. However, the nature of DNA methylation in the parasite remains unexplored. Read More

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http://dx.doi.org/10.1073/pnas.1917286117DOI Listing

The concerted action of two B3-like prophage genes exclude superinfecting bacteriophages by blocking DNA entry into

J Virol 2020 May 27. Epub 2020 May 27.

Departamento de Genética y Biología Molecular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV-IPN), Mexico City, Mexico.

In this study, we describe seven vegetative phage genomes homologous to the historic phage B3 that infect Like other phage groups, the B3-like group contains conserved (core) and variable (accessory) Open Reading Frames (ORFs) grouped at fixed regions in their genomes; however, in either case, many ORFs remain without assigned functions. We constructed lysogens of the seven B3-like phages in strain Ps33 of , a novel clinical isolate, and assayed the exclusion phenotype against a variety of temperate and virulent superinfecting phages. In addition to the classic exclusion conferred by the phage immunity repressor, the phenotype observed in B3-like lysogens suggested the presence of other exclusion genes. Read More

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http://dx.doi.org/10.1128/JVI.00953-20DOI Listing

New insights in the use of a strong cationic resin in dye adsorption.

Water Sci Technol 2020 Feb;81(4):773-780

Departamento de Ciencias del Ambiente, Facultad de Química y Biología, Universidad de Santiago de Chile, USACH, Casilla 40, Correo 33, Santiago, Chile E-mail:

The adsorption of methyl orange (MO) in aqueous solution was evaluated using a cationic polymer (Amberlite IRA 402) in batch experiments under different experimental variables such as amount of resin, concentration of MO, optimum interaction time and pH. The maximum adsorption capacity of the resin was 161.3 mg g at pH 7. Read More

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http://dx.doi.org/10.2166/wst.2020.158DOI Listing
February 2020

High mercury levels in Antarctic toothfish Dissostichus mawsoni from the Southwest Pacific sector of the Southern Ocean.

Environ Res 2020 May 16;187:109680. Epub 2020 May 16.

University of Coimbra, MARE - Marine and Environmental Sciences Centre, Department of Life Sciences, 3000-456, Coimbra, Portugal; British Antarctic Survey, Natural Environment Research Council, High Cross, Madingley Road, CB3 0ET, Cambridge, UK.

Mercury is a bioaccumulating toxic pollutant which can reach humans through the consumption of contaminated food (e.g. marine fish). Read More

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http://dx.doi.org/10.1016/j.envres.2020.109680DOI Listing

Human-iPSC-Derived Cardiac Stromal Cells Enhance Maturation in 3D Cardiac Microtissues and Reveal Non-cardiomyocyte Contributions to Heart Disease.

Cell Stem Cell 2020 May 20. Epub 2020 May 20.

Department of Anatomy and Embryology, Leiden University Medical Center, 2333 Leiden, the Netherlands; Department of Applied Stem Cell Technologies, University of Twente, 7500 Enschede, the Netherlands. Electronic address:

Cardiomyocytes (CMs) from human induced pluripotent stem cells (hiPSCs) are functionally immature, but this is improved by incorporation into engineered tissues or forced contraction. Here, we showed that tri-cellular combinations of hiPSC-derived CMs, cardiac fibroblasts (CFs), and cardiac endothelial cells also enhance maturation in easily constructed, scaffold-free, three-dimensional microtissues (MTs). hiPSC-CMs in MTs with CFs showed improved sarcomeric structures with T-tubules, enhanced contractility, and mitochondrial respiration and were electrophysiologically more mature than MTs without CFs. Read More

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http://dx.doi.org/10.1016/j.stem.2020.05.004DOI Listing

The role of nitric oxide in glutaric acid-induced convulsive behavior in pup rats.

Eur J Neurosci 2020 May 27. Epub 2020 May 27.

Laboratório de Síntese, Reatividade e Avaliação Farmacológica e Toxicológica de Organocalcogênios, Departamento de Bioquímica e Biologia Molecular, Centro de Ciências Naturais e Exatas, Federal University of Santa Maria (UFSM), Santa Maria, RS, 97105-900, Brazil.

Glutaric acidemia type I (GA-I) is a cerebral organic disorder characterized by the accumulation of glutaric acid (GA) and seizures. Since seizures are precipitated in children with GA-I and the mechanisms underlying this disorder are not well established, we decided to investigate the role of nitric oxide (NO) in GA-induced convulsive behavior in pup rats. Pup male Wistar rats (18-day-old) were anesthetized and placed in stereotaxic apparatus for cannula insertion into the striatum for injection of GA. Read More

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http://dx.doi.org/10.1111/ejn.14840DOI Listing

Identification of soybean trans-factors associated with plastid RNA editing sites.

Genet Mol Biol 2020 11;43(1 suppl 2):e20190067. Epub 2020 May 11.

Universidade Federal do Rio Grande do Sul (UFRGS), Centro de Biotecnologia, Programa de Pós-Graduação em Biologia Celular e Molecular (PPGBCM), Porto Alegre, RS, Brazil.

RNA editing is a posttranscriptional process that changes nucleotide sequences, among which cytosine-to-uracil by a deamination reaction can revert non-neutral codon mutations. Pentatricopeptide repeat (PPR) proteins comprise a family of RNA-binding proteins, with members acting as editing trans-factors that recognize specific RNA cis-elements and perform the deamination reaction. PPR proteins are classified into P and PLS subfamilies. Read More

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http://dx.doi.org/10.1590/1678-4685-GMB-2019-0067DOI Listing

Colonization and Transmission in Oil Palm () Plantations in the Orinoco Basin, Colombia.

Am J Trop Med Hyg 2020 May 26. Epub 2020 May 26.

Grupo de Investigación en Biología Matemática y Computacional (BIOMAC), Universidad de los Andes, Bogotá, Colombia.

is the etiological agent of Chagas disease that infects more than seven million people in Latin America. The parasite is transmitted by triatomine insects, of which some species are often associated with palms. The establishment of oil palm plantations () in the Orinoco region (Colombia) has been rapidly growing, possibly constituting a new environment for the establishment and increase in triatomine populations. Read More

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http://dx.doi.org/10.4269/ajtmh.19-0331DOI Listing

Potential negative effects of the free use of chloroquine to manage COVID-19 in Colombia.

J Med Virol 2020 May 26. Epub 2020 May 26.

Grupo de Investigaciones Microbiológicas-UR (GIMUR), Departamento de Biología, Facultad de Ciencias Naturales, Universidad del Rosario, Colombia.

Coronavirus disease 2019 (COVID-19) pandemic has challenged healthcare systems around the world. Unfortunately, failure has ensued: high-income countries have succumbed to the global emergency despite highly prepared human and technological assets. There is no current consensus on pharmacological management of COVID-19, but chloroquine phosphate (CQ) has emerged as a possible therapeutical candidate. Read More

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http://dx.doi.org/10.1002/jmv.26059DOI Listing

Maintenance of photosynthetic capacity in flooded tomato plants with reduced ethylene sensitivity.

Physiol Plant 2020 May 27. Epub 2020 May 27.

Instituto de Botánica del Nordeste (UNNE-CONICET), 2131 Sargento Cabral, CP 3400, Corrientes, Argentina.

Ethylene is considered one of the most important plant hormones orchestrating plant responses to flooding stress. However, ethylene may induce deleterious effects on plants, especially when produced at high rates in response to stress. In this paper, we explored the effect of attenuated ethylene sensitivity in the Never ripe (Nr) mutant on leaf photosynthetic capacity of flooded tomato plants. Read More

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http://dx.doi.org/10.1111/ppl.13141DOI Listing

Caveolin-1 function at the plasma membrane and in intracellular compartments in cancer.

Cancer Metastasis Rev 2020 May 27. Epub 2020 May 27.

Laboratory of Cellular Communication, Center for studies on Exercise, Metabolism and Cancer (CEMC), Programa de Biología Celular y Molecular, Facultad de Medicina, Universidad de Chile, Santiago, Chile.

Caveolin-1 (CAV1) is commonly considered to function as a cell surface protein, for instance in the genesis of caveolae. Nonetheless, it is also present in many intracellular organelles and compartments. The contributions of these intracellular pools to CAV1 function are generally less well understood, and this is also the case in the context of cancer. Read More

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http://dx.doi.org/10.1007/s10555-020-09890-xDOI Listing

Precision Health for Chagas Disease: Integrating Parasite and Host Factors to Predict Outcome of Infection and Response to Therapy.

Front Cell Infect Microbiol 2020 8;10:210. Epub 2020 May 8.

Department of Pathology and Laboratory Medicine, Cedars Sinai Medical Center, Los Angeles, CA, United States.

Chagas disease, caused by the infection with the protozoan parasite , is clinically manifested in approximately one-third of infected people by inflammatory heart disease (cardiomyopathy) and, to a minor degree, gastrointestinal tract disorders (megaesophagus or megacolon). Chagas disease is a zoonosis transmitted among animals and people through the contact with triatomine bugs, which are found in much of the western hemisphere, including most countries of North, Central and South America, between parallels 45° north (Minneapolis, USA) and south (Chubut Province, Argentina). Despite much research on drug discovery for , there remain only two related agents in widespread use. Read More

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http://dx.doi.org/10.3389/fcimb.2020.00210DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7225773PMC

Development of Nanobodies Against Hemorrhagic and Myotoxic Components of Snake Venom.

Front Immunol 2020 7;11:655. Epub 2020 May 7.

Cellular and Molecular Immunology, Vrije Universiteit Brussel, Brussels, Belgium.

Snake envenoming is a globally neglected public health problem. Antivenoms produced using animal hyperimmune plasma remain the standard therapy for snakebites. Although effective against systemic effects, conventional antivenoms have limited efficacy against local tissue damage. Read More

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http://dx.doi.org/10.3389/fimmu.2020.00655DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7224310PMC

Editorial: Risk Factors for Alzheimer's Disease.

Front Aging Neurosci 2020 7;12:124. Epub 2020 May 7.

Departamento Biologia Celular, Genetica y Fisiologia, Facultad de Ciencias, Instituto de Investigacion Biomedica de Malaga-IBIMA, Universidad de Malaga, Málaga, Spain.

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http://dx.doi.org/10.3389/fnagi.2020.00124DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7221022PMC

Evolutionary analyses reveal independent origins of gene repertoires and structural motifs associated to fast inactivation in calcium-selective TRPV channels.

Sci Rep 2020 May 26;10(1):8684. Epub 2020 May 26.

Millennium Nucleus of Ion Channels-Associated Diseases (MiNICAD), Valdivia, Chile.

Essential for calcium homeostasis, TRPV5 and TRPV6 are calcium-selective channels belonging to the transient receptor potential (TRP) gene family. In this study, we investigated the evolutionary history of these channels to add an evolutionary context to the already available physiological information. Phylogenetic analyses revealed that paralogs found in mammals, sauropsids, amphibians, and chondrichthyes, are the product of independent duplication events in the ancestor of each group. Read More

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http://dx.doi.org/10.1038/s41598-020-65679-6DOI Listing

Genomic adaptations to aquatic and aerial life in mayflies and the origin of insect wings.

Nat Commun 2020 May 26;11(1):2631. Epub 2020 May 26.

GEM-DMC2 Unit, The CABD (CSIC-UPO-JA), Ctra. de Utrera km 1, 41013, Seville, Spain.

The evolution of winged insects revolutionized terrestrial ecosystems and led to the largest animal radiation on Earth. However, we still have an incomplete picture of the genomic changes that underlay this diversification. Mayflies, as one of the sister groups of all other winged insects, are key to understanding this radiation. Read More

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http://dx.doi.org/10.1038/s41467-020-16284-8DOI Listing

Understanding the metabolism of the tetralin degrader Sphingopyxis granuli strain TFA through genome-scale metabolic modelling.

Sci Rep 2020 May 26;10(1):8651. Epub 2020 May 26.

Department of Molecular Biology and Biochemical Engineering. Universidad Pablo de Olavide, ES-41013, Seville, Spain.

Sphingopyxis granuli strain TFA is an α-proteobacterium that belongs to the sphingomonads, a group of bacteria well-known for its degradative capabilities and oligotrophic metabolism. Strain TFA is the only bacterium in which the mineralisation of the aromatic pollutant tetralin has been completely characterized at biochemical, genetic, and regulatory levels and the first Sphingopyxis characterised as facultative anaerobe. Here we report additional metabolic features of this α-proteobacterium using metabolic modelling and the functional integration of genomic and transcriptomic data. Read More

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http://dx.doi.org/10.1038/s41598-020-65258-9DOI Listing

Past and future decline of tropical pelagic biodiversity.

Proc Natl Acad Sci U S A 2020 May 26. Epub 2020 May 26.

Faculty of Science, University of the Ryukyus, 903-0213 Okinawa, Japan.

A major research question concerning global pelagic biodiversity remains unanswered: when did the apparent tropical biodiversity depression (i.e., bimodality of latitudinal diversity gradient [LDG]) begin? The bimodal LDG may be a consequence of recent ocean warming or of deep-time evolutionary speciation and extinction processes. Read More

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http://dx.doi.org/10.1073/pnas.1916923117DOI Listing

Vitamin D-mitochondria cross-talk could modulate the signaling pathway involved in hypertension development: a translational integrative overview.

Clin Investig Arterioscler 2020 May 23. Epub 2020 May 23.

Laboratorio de Farmacología Experimental Básica y Traslacional, Área de Farmacología, Departamento de Patología, Facultad de Ciencias Médicas, Universidad Nacional de Cuyo, Centro Universitario, Mendoza, Argentina; Instituto de Medicina y Biología Experimental de Cuyo, Consejo Nacional de Investigación Científica y Tecnológica (IMBECU-CONICET), Mendoza, Argentina. Electronic address:

Vitamin D deficiency is a worldwide pandemic and results in osteoporosis, hypertension, and other cardiovascular diseases. At the cellular level, it produces significant oxidative stress, inflammatory markers, and mitochondrial damage. There is increasing evidence about the role of vitamin D in the regulation of the renin-angiotensin-aldosterone system (RAAS). Read More

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http://dx.doi.org/10.1016/j.arteri.2020.02.002DOI Listing

Mechanisms of Linezolid Resistance Among Clinical spp. in Spain: Spread of Methicillin- and Linezolid-Resistant ST2.

Microb Drug Resist 2020 May 22. Epub 2020 May 22.

Área de Bioquímica y Biología Molecular, Universidad de La Rioja, Logroño, Spain.

This study aimed at determining the mechanisms of linezolid resistance and the molecular characteristics of clinical ( = 2) and coagulase-negative staphylococci ( = 15) isolates obtained from four Spanish hospitals. The detection of linezolid resistance mechanisms (mutations and acquisition of resistance genes) was performed by PCR/sequencing. The antimicrobial resistance and virulence profile was determined, and the isolates were typed by different molecular techniques. Read More

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http://dx.doi.org/10.1089/mdr.2020.0122DOI Listing

Phylogeny of () (Diptera: Culicidae) Using Mitochondrial Genes.

Insects 2020 May 24;11(5). Epub 2020 May 24.

Departamento de Epidemiologia, Faculdade de Saúde Pública, Universidade de São Paulo, São Paulo, SP 01246-904, Brazil.

Identification of mosquito species is necessary for determining the entomological components of malaria transmission, but it can be difficult in morphologically similar species. DNA sequences are largely used as an additional tool for species recognition, including those that belong to species complexes. mosquitoes are vectors of human and simian malaria in the Neotropical Region, but there are few DNA sequences of species in public databases. Read More

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http://dx.doi.org/10.3390/insects11050324DOI Listing

Reduced Graphene Oxide Nanosheet-Decorated Copper Oxide Nanoparticles: A Potent Antifungal Nanocomposite against Fusarium Root Rot and Wilt Diseases of Tomato and Pepper Plants.

Nanomaterials (Basel) 2020 May 24;10(5). Epub 2020 May 24.

Mycology and Disease Survey Research Department, Plant Pathology Research Institute, Agricultural Research Center, Giza 12619, Egypt.

Sustainable use of nanotechnology in crop protection requires an understanding of the plant's life cycle, potential toxicological impacts of nanomaterials and their mechanism of action against the target pathogens. Herein, we show some properties of a candidate antifungal nanocomposite made from copper oxide (CuO; otherwise an essential soil nutrient) nanoparticles (NPs), with definite size and shape, decorating the surface of reduced graphene oxide (rGO) nanosheets. The successful preparation of the rGO-CuO NPs was confirmed by spectroscopic and microscopic analyses, and its antifungal activity against wild strains of affecting tomato and pepper plants was successfully confirmed. Read More

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http://dx.doi.org/10.3390/nano10051001DOI Listing

Pleiotropic Roles of Calmodulin in the Regulation of KRas and Rac1 GTPases: Functional Diversity in Health and Disease.

Int J Mol Sci 2020 May 23;21(10). Epub 2020 May 23.

School of Pharmacy, Faculty of Medicine and Health, University of Sydney, Sydney, NSW 2006, Australia.

Calmodulin is a ubiquitous signalling protein that controls many biological processes due to its capacity to interact and/or regulate a large number of cellular proteins and pathways, mostly in a Ca-dependent manner. This complex interactome of calmodulin can have pleiotropic molecular consequences, which over the years has made it often difficult to clearly define the contribution of calmodulin in the signal output of specific pathways and overall biological response. Most relevant for this review, the ability of calmodulin to influence the spatiotemporal signalling of several small GTPases, in particular KRas and Rac1, can modulate fundamental biological outcomes such as proliferation and migration. Read More

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http://dx.doi.org/10.3390/ijms21103680DOI Listing

Potential Application of Whole Body Vibration Exercise For Improving The Clinical Conditions of COVID-19 Infected Individuals: A Narrative Review From the World Association of Vibration Exercise Experts (WAVex) Panel.

Int J Environ Res Public Health 2020 05 22;17(10). Epub 2020 May 22.

Laboratório de Vibrações Mecânicas, Policlínica Piquet Carneiro, Instituto de Biología Roberto Alcantara Gomes, Universidade do Estado do Rio de Janeiro, Rio de Janeiro 20950-003, Brazil.

COVID-19 is a highly infectious respiratory disease which leads to several clinical conditions related to the dysfunction of the respiratory system along with other physical and psychological complaints. Severely affected patients are referred to intensive care units (ICUs), limiting their possibilities for physical exercise. Whole body vibration (WBV) exercise is a non-invasive, physical therapy, that has been suggested as part of the procedures involved with pulmonary rehabilitation, even in ICU settings. Read More

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http://dx.doi.org/10.3390/ijerph17103650DOI Listing
May 2020
2.063 Impact Factor

Evaluation of Product Distribution in Chemostat and Batch Fermentation in Lactic Acid-Producing Strains Utilizing Glycerol as Substrate.

Microorganisms 2020 May 22;8(5). Epub 2020 May 22.

Pós-Graduação em Ciências Genômicas e Biotecnologia, Universidade Católica de Brasília, Brasília DF CEP 70790-160, Brazil.

Lactic acid is the monomeric unit of polylactide (PLA), a bioplastic widely used in the packaging, automotive, food, and pharmaceutical industries. Previously, the yeast was genetically modified for the production of lactate from glycerol. For this, the bovine L-lactate dehydrogenase- (LDH)-encoding gene was inserted and the gene encoding the pyruvate decarboxylase (PDC) was disrupted, resulting in the GLp strain. Read More

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http://dx.doi.org/10.3390/microorganisms8050781DOI Listing

Phage Display Detection of Mimotopes that Are Shared Epitopes of Clinically and Epidemiologically Relevant Enterobacteria.

Microorganisms 2020 May 22;8(5). Epub 2020 May 22.

Departamento de Salud Pública, Facultad de Medicina, Universidad Nacional Autónoma de México, México City CP 04510, Mexico.

Background: and are etiologic agents of intestinal infections. A previous study showed the presence of shared epitopes between lipopolysaccharides (LPSs) of O157 and .

Aim: Using phage display, the aim of this study is to identify mimotopes of shared epitopes in different enterobacterial LPSs. Read More

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http://dx.doi.org/10.3390/microorganisms8050780DOI Listing

Synthesis of a Small Library of Nature-Inspired Xanthones and Study of Their Antimicrobial Activity.

Molecules 2020 May 21;25(10). Epub 2020 May 21.

CIIMAR - Centro Interdisciplinar de Investigação Marinha e Ambiental, Terminal de Cruzeiros do Porto de Leixões, 4450-208 Matosinhos, Portugal.

A series of thirteen xanthones - was prepared based on substitutional (appendage) diversity reactions. The series was structurally characterized based on their spectral data and HRMS, and the structures of xanthone derivatives , , and were determined by single-crystal X-ray diffraction. This series, along with an in-house series of aminated xanthones - was tested for in-vitro antimicrobial activity against seven bacterial (including two multidrug-resistant) strains and five fungal strains. Read More

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http://dx.doi.org/10.3390/molecules25102405DOI Listing

Bifunctional Tm,Yb:GdVO@SiO Core-Shell Nanoparticles in HeLa Cells: Upconversion Luminescence Nanothermometry in the First Biological Window and Biolabelling in the Visible.

Nanomaterials (Basel) 2020 May 21;10(5). Epub 2020 May 21.

Fisica i Cristalografia de Materials i Nanomaterials (FiCMA-FiCNA)-EMaS, Universitat Rovira i Virgili (URV), Campus Sescelades, Marcelli Domingo 1, E-43007 Tarragona, Spain.

The bifunctional possibilities of Tm,Yb:GdVO@SiO core-shell nanoparticles for temperature sensing by using the near-infrared (NIR)-excited upconversion emissions in the first biological window, and biolabeling through the visible emissions they generate, were investigated. The two emission lines located at 700 and 800 nm, that arise from the thermally coupled F and H energy levels of Tm, were used to develop a luminescent thermometer, operating through the Fluorescence Intensity Ratio () technique, with a very high thermal relative sensitivity . Moreover, since the inert shell surrounding the luminescent active core allows for dispersal of the nanoparticles in water and biological compatible fluids, we investigated the penetration depth that can be realized in biological tissues with their emissions in the NIR range, achieving a value of 0. Read More

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http://dx.doi.org/10.3390/nano10050993DOI Listing

Comparative Genomics of the Genus Shows Wide Distribution of Biodegradation Traits.

Microorganisms 2020 May 21;8(5). Epub 2020 May 21.

Departamento de Biología, Facultad de Ciencias, Universidad Autónoma de Madrid, Darwin 2, 28049 Madrid, Spain.

The genus exhibits great potential for bioremediation applications due to its huge metabolic diversity, including biotransformation of aromatic and aliphatic compounds. Comparative genomic studies of this genus are limited to a small number of genomes, while the high number of sequenced strains to date could provide more information about the diversity. Phylogenomic analysis of 327 genomes and clustering of intergenomic distances identified 42 phylogenomic groups and 83 species-level clusters. Read More

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http://dx.doi.org/10.3390/microorganisms8050774DOI Listing

Melatonin from an Antioxidant to a Classic Hormone or a Tissue Factor: Experimental and Clinical Aspects 2019.

Int J Mol Sci 2020 May 21;21(10). Epub 2020 May 21.

Departamento de Morfología y Biología Celular, Redox Biology Group, Instituto Universitario Oncológico del Principado de Asturias (IUOPA), School of Medicine, University of Oviedo. C/ Julián Clavería 6, 33006 Oviedo, Spain.

During the last 25 years we have accomplished great advances in melatonin research, regarding antioxidant or anti-inflammatory functions, oncostatic actions, glucose metabolism regulation or plant physiology, among others. Of course, we should not forget the classical, circadian-related functions of the indole, which has recently brought up new and important findings. All together these new discoveries will likely lead the way in the next decade in terms of melatonin research. Read More

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http://dx.doi.org/10.3390/ijms21103645DOI Listing

One-Week High-Intensity Interval Training Increases Hippocampal Plasticity and Mitochondrial Content without Changes in Redox State.

Antioxidants (Basel) 2020 May 21;9(5). Epub 2020 May 21.

Departamento de Ciências BioMoleculares, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, São Paulo 14040-903, Brasil.

Evidence suggests that physical exercise has effects on neuronal plasticity as well as overall brain health. This effect has been linked to exercise capacity in modulating the antioxidant status, when the oxidative stress is usually linked to the neuronal damage. Although high-intensity interval training (HIIT) is the training-trend worldwide, its effect on brain function is still unclear. Read More

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http://dx.doi.org/10.3390/antiox9050445DOI Listing

Chitosan-Based Drug Delivery System: Applications in Fish Biotechnology.

Polymers (Basel) 2020 May 21;12(5). Epub 2020 May 21.

Secció de Bioquímica i Biologia Molecular, Departament de Bioquímica i Fisiologia, Facultat de Farmàcia i Ciències de l'Alimentació, Universitat de Barcelona, Joan XXIII 27-31, 08028 Barcelona, Spain.

Chitosan is increasingly used for safe nucleic acid delivery in gene therapy studies, due to well-known properties such as bioadhesion, low toxicity, biodegradability and biocompatibility. Furthermore, chitosan derivatization can be easily performed to improve the solubility and stability of chitosan-nucleic acid polyplexes, and enhance efficient target cell drug delivery, cell uptake, intracellular endosomal escape, unpacking and nuclear import of expression plasmids. As in other fields, chitosan is a promising drug delivery vector with great potential for the fish farming industry. Read More

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http://dx.doi.org/10.3390/polym12051177DOI Listing