81,794 results match your criteria Biologia Plantarum[Journal]


Ecotoxicological effects of lanthanum in Mytilus galloprovincialis: Biochemical and histopathological impacts.

Aquat Toxicol 2019 Mar 25;211:181-192. Epub 2019 Mar 25.

Departamento de Biologia & CESAM, Universidade de Aveiro, 3810-193 Aveiro, Portugal. Electronic address:

Inappropriate processing and disposal of electronic waste contributes to the contamination of aquatic systems by various types of pollutants such as the rare-earth elements (REE) in which lanthanum (La) is included. Knowledge on the toxicity of these elements in marine organisms is still scarce when compared to other metals such as mercury (Hg) and arsenic (As). Therefore, this study aims to assess the toxicity of La on the mussel Mytilus galloprovincialis, considered a good bioindicator of aquatic pollution, through the analysis of metabolic, oxidative stress, neurotoxicity and histopathological markers. Read More

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http://dx.doi.org/10.1016/j.aquatox.2019.03.017DOI Listing

A comparative study of the pharyngeal plate of Apoidea (Hymenoptera: Aculeata), with implications for the understanding of phylogenetic relationships of bees.

Arthropod Struct Dev 2019 Apr 16. Epub 2019 Apr 16.

Laboratório de Biologia Comparada e Abelhas (LBCA) - Departamento de Biologia, Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto (FFCLRP), Universidade de São Paulo, 14040-901, Ribeirão Preto, SP, Brazil. Electronic address:

The pharyngeal plate is a morphological complex with extensive anatomical variation among bees and, therefore, potential as a source of phylogenetic information. The pharyngeal plate of bees is divided into four morphologically distinct regions: sitophore, hypopharyngeal lobe, pharyngeal rods, and median oral plate. In this work we illustrate and document in detail for the first time the pharyngeal plate of 44 bee species, providing descriptions of the morphological variation and contrasting these findings with representatives of apoid wasps (Crabronidae and Sphecidae). Read More

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http://dx.doi.org/10.1016/j.asd.2019.04.002DOI Listing

Mitochondrial genome characterization of Melipona bicolor: Insights from the control region and gene expression data.

Gene 2019 Apr 16. Epub 2019 Apr 16.

Universidade de São Paulo, Instituto de Biociências - Departamento de Genética e Biologia Evolutiva, Sala 320, Rua do Matão, 277, Cidade Universitária, São Paulo, SP 05508-900, Brazil.

The stingless bee Melipona bicolor is the only bee in which true polygyny occurs. Its mitochondrial genome was first sequenced in 2008, but it was incomplete and no information about its transcription was known. We combined short and long reads of M. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S03781119193039
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http://dx.doi.org/10.1016/j.gene.2019.04.042DOI Listing
April 2019
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Proteomic characterization of human sperm plasma membrane-associated proteins and their role in capacitation.

Andrology 2019 Apr 19. Epub 2019 Apr 19.

Departamento de Biología de la Reproducción Dr. Carlos Gual Castro, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Ciudad de México, México.

Background: Plasma membranes of ejaculated sperm are covered by epididymal and accessory glands secreted proteins that must be released from sperm surface during the female reproductive tract passage in order to capacitate and fertilize the oocyte.

Objectives: As human sperm plasma membrane-associated proteins (SMAP) have not yet been investigated, the aim of this study was to characterize the SMAP released during in vitro human capacitation and to study their possible role as decapacitation factors.

Materials And Methods: SMAP were characterized by 2-dimensional electrophoresis and mass spectrometry analysis. Read More

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http://dx.doi.org/10.1111/andr.12627DOI Listing

A landscape-scale assessment of tropical mammals reveals the effects of habitat and anthropogenic disturbance on community occupancy.

PLoS One 2019 19;14(4):e0215682. Epub 2019 Apr 19.

Tropical Biodiversity Section, MUSE-Museo delle Scienze, Trento, Italy.

With biodiversity facing unparalleled threats from anthropogenic disturbance, knowledge on the occurrences of species and communities provides for an effective and fast approach to assess their status and vulnerability. Disturbance is most prominent at the landscape-level, for example through habitat loss from large-scale resource extraction or agriculture. However, addressing species responses to habitat changes at the landscape-scale can be difficult and cost-ineffective, hence studies are mostly conducted at single areas or habitat patches. Read More

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0215682PLOS

Inhibition of Tityus serrulatus venom hyaluronidase affects venom biodistribution.

PLoS Negl Trop Dis 2019 Apr 19;13(4):e0007048. Epub 2019 Apr 19.

Mestrado Profissional em Biotecnologia e Gestão da Inovação, Centro Universitário de Sete Lagoas, Sete Lagoas, Minas Gerais, Brazil.

Background: The hyaluronidase enzyme is generally known as a spreading factor in animal venoms. Although its activity has been demonstrated in several organisms, a deeper knowledge about hyaluronidase and the venom spreading process from the bite/sting site until its elimination from the victim's body is still in need. Herein, we further pursued the goal of demonstrating the effects of inhibition of T. Read More

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http://dx.plos.org/10.1371/journal.pntd.0007048
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http://dx.doi.org/10.1371/journal.pntd.0007048DOI Listing
April 2019
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Micro- and macroparasite species richness in birds: the role of host life history and ecology.

J Anim Ecol 2019 Apr 19. Epub 2019 Apr 19.

NIOZ Royal Netherlands Institute for Sea Research, Department of Coastal Systems, and Utrecht University, P.O. Box 59, 1790 AB, Den Burg, Texel, Netherlands.

1.Identifying the factors shaping variation in parasite diversity among host species is crucial to understand wildlife diseases. Although micro- and macroparasites may exert different selective pressures on their hosts, studies investigating the determinants of parasite species richness in animals have rarely considered this divide. Read More

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http://dx.doi.org/10.1111/1365-2656.12998DOI Listing

DNA barcoding uncovers extensive cryptic diversity in the African long-fin tetra Bryconalestes longipinnis (Alestidae: Characiformes).

J Fish Biol 2019 Apr 19. Epub 2019 Apr 19.

Department of Ichthyology, American Museum of Natural History, New York, New York, USA.

To investigate the presence of cryptic diversity in The African longfin-tetra Bryconalestes longipinnis, we employed DNA barcoding in a phylogeographic context, as well as geometric morphometrics, documenting for the first time genetic and body shape variation in the species. Analysis of cytochrome oxidase I gene (coI) sequence variation exposed extremely high levels of genetic differentiation among samples from across the geographic range of the species (up to 18%), certainly much greater than the traditionally employed c. 3% sequence divergence heuristic threshold for conspecifics. Read More

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http://dx.doi.org/10.1111/jfb.13987DOI Listing

Genome sequencing of Burkholderia contaminans LTEB11 reveals a lipolytic arsenal of biotechnological interest.

Braz J Microbiol 2019 Apr 18. Epub 2019 Apr 18.

Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, Cx. P. 19046 Centro Politécnico, Curitiba, Paraná, 81531-980, Brazil.

Burkholderia contaminans LTEB11 is a Gram-negative betaproteobacterium isolated as a contaminant of a culture in mineral medium supplemented with vegetable oil. Here, we report the genome sequence of B. contaminans LTEB11, identifying and analyzing the genes involved in its lipolytic machinery and in the production of other biotechnological products. Read More

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http://dx.doi.org/10.1007/s42770-019-00076-4DOI Listing

Spread of the serotypes and antimicrobial resistance in strains of Salmonella spp. isolated from broiler.

Braz J Microbiol 2019 Apr 18. Epub 2019 Apr 18.

Laboratório de Biotecnologia Animal Aplicada, Faculdade de Medicina Veterinária, Universidade Federal de Uberlândia, Rua Ceará, s/n, Bloco 2D, Sala 43, Campus Umuarama, Uberlândia, MG, Brazil.

Serotypes and antimicrobial susceptibility of Salmonella's isolates from broiler production chain were determined. A total of 239 isolated strains from chicken, carcasses, breeding environments, and slaughter was analyzed by disk diffusion test, in the period of 2009 to 2010. For antibiotics with a high number of resistant strains, the minimal inhibitory concentration (MIC) was performed. Read More

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http://dx.doi.org/10.1007/s42770-019-00054-wDOI Listing

Behavioral and biochemical effects of the antifouler and antidandruff zinc pyrithione on the freshwater fish Gambusia holbrooki.

Fish Physiol Biochem 2019 Apr 18. Epub 2019 Apr 18.

Departamento de Biologia/CESAM, Universidade de Aveiro, Campus de Santiago, 3810-193, Aveiro, Portugal.

The presence of pharmaceutical residues in the aquatic environment is receiving great attention since the levels of these substances have significantly increased in this compartment, potentially leading to adverse ecological effects. Zinc pyrithione (ZnPt) is a widely used organometallic biocide, which is incorporated into antifouling formulas, such as paints, to prevent the establishment of biofilms on surfaces exposed to the aquatic environment. It is also used in cosmetics, such as antidandruff shampoos and soaps. Read More

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http://link.springer.com/10.1007/s10695-019-00634-5
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http://dx.doi.org/10.1007/s10695-019-00634-5DOI Listing
April 2019
2 Reads

Novel Antibacterial Resin-Based Filling Material Containing Nanoparticles for the Potential One-Step Treatment of Caries.

J Healthc Eng 2019 13;2019:6367919. Epub 2019 Mar 13.

Unidad de Investigación y Desarrollo en Tecnología Farmacéutica (UNITEFA), CONICET y Departamento de Ciencias Farmacéuticas, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Ciudad Universitaria, X5000-Córdoba, Argentina.

The aim of this work was to study the application of resin filling containing nanomaterials for the potential treatment of caries. Zinc nanoparticles (ZnO@NP, 50 nm) were chosen for their antimicrobial capacity against aerobic bacteria, and here, they have proved to be bactericidal against anaerobic bacterial strains (, , and spp.). Read More

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http://dx.doi.org/10.1155/2019/6367919DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6436334PMC

Mutations From Whole Exome Sequencing in Neurodevelopmental and Psychiatric Disorders: From Discovery to Application.

Front Genet 2019 3;10:258. Epub 2019 Apr 3.

Shanghai Mental Health Center, School of Biomedical Engineering, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Neurodevelopmental and psychiatric disorders are a highly disabling and heterogeneous group of developmental and mental disorders, resulting from complex interactions of genetic and environmental risk factors. The nature of multifactorial traits and the presence of comorbidity and polygenicity in these disorders present challenges in both disease risk identification and clinical diagnoses. The genetic component has been firmly established, but the identification of all the causative variants remains elusive. Read More

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https://www.frontiersin.org/article/10.3389/fgene.2019.00258
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http://dx.doi.org/10.3389/fgene.2019.00258DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6456656PMC
April 2019
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The Altered Expression of Influences the Arabidopsis Response to Iron Deficiency.

Front Plant Sci 2019 2;10:324. Epub 2019 Apr 2.

Departament de Bioquímica i Biologia Molecular, Estructura de Recerca Interdisciplinar en Biotecnologiaia i Biomedicina (ERI BIOTECMED), Universitat de València, Valencia, Spain.

MicroRNAs contribute to the adaptation of plants to varying environmental conditions by affecting systemic mineral nutrient homeostasis. Copper and iron deficiencies antagonistically control the expression of (), which post-transcriptionally regulates laccase-like multicopper oxidase family members , , and . In this work, we used T-DNA insertion mutants (408-KO1 and 408-KO2) and a previously characterized transgenic line overexpressing (35S:408-14) to explore how influences copper- and iron-dependent metabolism. Read More

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https://www.frontiersin.org/article/10.3389/fpls.2019.00324/
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http://dx.doi.org/10.3389/fpls.2019.00324DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6454987PMC
April 2019
1 Read

The Structure and Occurrence of a Velum in Traps ().

Front Plant Sci 2019 22;10:302. Epub 2019 Mar 22.

Unit of Botany and Plant Physiology, Institute of Plant Biology and Biotechnology, University of Agriculture in Kraków, Cracow, Poland.

Bladderworts (, , Lamiales) are carnivorous plants that form small suction traps (bladders) for catching invertebrates. The velum is a cuticle structure that is produced by specialized trichomes of the threshold pavement epithelium. It is believed that the velum together with the mucilage seals the free edge of the trap door and that it is necessary for correct functioning of the trap. Read More

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https://www.frontiersin.org/article/10.3389/fpls.2019.00302/
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http://dx.doi.org/10.3389/fpls.2019.00302DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6454230PMC
March 2019
1 Read

Akt/AS160 Signaling Pathway Inhibition Impairs Infection by Decreasing Rab14-Controlled Sphingolipids Delivery to Chlamydial Inclusions.

Front Microbiol 2019 3;10:666. Epub 2019 Apr 3.

Laboratorio de Bioquímica e Inmunidad, Área de Química Biológica, Facultad de Ciencias Médicas, Universidad Nacional de Cuyo, Mendoza, Argentina.

, an obligate intracellular bacterium, intercepts different trafficking pathways of the host cell to acquire essential lipids for its survival and replication, particularly from the Golgi apparatus via a Rab14-mediated transport. Molecular mechanisms underlying how these bacteria manipulate intracellular transport are a matter of intense study. Here, we show that utilizes Akt/AS160 signaling pathway to promote sphingolipids delivery to the chlamydial inclusion through Rab14-controlled vesicular transport. Read More

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http://dx.doi.org/10.3389/fmicb.2019.00666DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6456686PMC

Estradiol-Mediated Axogenesis of Hypothalamic Neurons Requires ERK1/2 and Ryanodine Receptors-Dependent Intracellular Ca Rise in Male Rats.

Front Cell Neurosci 2019 2;13:122. Epub 2019 Apr 2.

Instituto de Investigación Médica Mercedes y Martín Ferreyra, INIMEC-CONICET, Universidad Nacional de Córdoba, Córdoba, Argentina.

17β-estradiol (E2) induces axonal growth through extracellular signal-regulated kinase 1 and 2 (ERK1/2)-MAPK cascade in hypothalamic neurons of male rat embryos , but the mechanism that initiates these events is poorly understood. This study reports the intracellular Ca increase that participates in the activation of ERK1/2 and axogenesis induced by E2. Hypothalamic neuron cultures were established from 16-day-old male rat embryos and fed with astroglia-conditioned media for 48 h. Read More

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http://dx.doi.org/10.3389/fncel.2019.00122DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6454002PMC

The response of Sphingopyxis granuli strain TFA to the hostile anoxic condition.

Sci Rep 2019 Apr 18;9(1):6297. Epub 2019 Apr 18.

Centro Andaluz de Biología del Desarrollo/CSIC/Universidad Pablo de Olavide/Junta de Andalucía. Departamento de Biología Molecular e Ingeniería Bioquímica, Seville, Spain.

Sphingomonads comprises a group of interesting aerobic bacteria because of their ubiquity and metabolic capability of degrading many recalcitrant contaminants. The tetralin-degrader Sphingopyxis granuli strain TFA has been recently reported as able to anaerobically grow using nitrate as the alternative electron acceptor and so far is the only bacterium with this ability within the sphingomonads group. To understand how strain TFA thrives under anoxic conditions, a differential transcriptomic analysis while growing under aerobic or anoxic conditions was performed. Read More

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http://www.nature.com/articles/s41598-019-42768-9
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http://dx.doi.org/10.1038/s41598-019-42768-9DOI Listing
April 2019
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Draft Genome Sequence of Paraburkholderia sp. UYCP14C, a Rhizobium Strain Isolated from Root Nodules of Calliandra parvifolia.

Microbiol Resour Announc 2019 Apr 18;8(16). Epub 2019 Apr 18.

Departmento de Bioquímica y Genómica Microbianas, Instituto de Investigaciones Biológicas Clemente Estable, MEC, Montevideo, Uruguay

Here, we present the draft genome sequence of strain UYCP14C, a rhizobium isolated from nodules. The assembled genome size was around 9.8 million bp, containing 9,031 predicted protein-coding sequences, including several symbiotic and nitrogen fixation genes. Read More

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http://dx.doi.org/10.1128/MRA.00173-19DOI Listing

Genomic Islands in the Full-Genome Sequence of an NAD-Hemin-Independent Avibacterium paragallinarum Strain Isolated from Peru.

Microbiol Resour Announc 2019 Apr 18;8(16). Epub 2019 Apr 18.

Laboratorios de Investigación y Desarrollo, FARVET, Chincha Alta, Ica, Peru.

Here, we report the full-genome sequence of an NAD-hemin-independent serovar C-2 strain, FARPER-174, isolated from layer hens in Peru. This genome contained 12 potential genomic islands that include ribosomal protein-coding genes, a gene, hemocin-coding genes, sequences of fagos, an operon, and drug resistance genes. Read More

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http://dx.doi.org/10.1128/MRA.00118-19DOI Listing

Draft Genome Sequences of Sporulation-Impaired Bacillus pumilus Strain NRS576 and Its Native Plasmid p576.

Microbiol Resour Announc 2019 Apr 18;8(16). Epub 2019 Apr 18.

Department of Virology and Microbiology, Centro de Biología Molecular "Severo Ochoa" (CSIC-UAM), Instituto de Biología Molecular "Eladio Viñuela" (CSIC), Universidad Autónoma Madrid, Madrid, Spain

spores can cause foodborne poisonings. strain NRS576 forms spores with a very reduced efficiency due to the presence of a plasmid, named p576. Here, we report the genome sequence of strain NRS576 and its plasmid p576. Read More

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http://dx.doi.org/10.1128/MRA.00089-19DOI Listing

Draft Genome Sequence of sp. Strain T2.26MG-98, Isolated from 492.6 Meters Deep on the Subsurface of the Iberian Pyrite Belt.

Microbiol Resour Announc 2019 Apr 18;8(16). Epub 2019 Apr 18.

Centro de Biología Molecular Severo Ochoa (CBMSO, CSIC-UAM), Universidad Autónoma de Madrid, Cantoblanco, Madrid, Spain

sp. strain T2.26MG-98 was isolated from the deep subsurface of the Iberian Pyrite Belt. Read More

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http://dx.doi.org/10.1128/MRA.00070-19DOI Listing

A User's Guide to Metaphors In Ecology and Evolution.

Trends Ecol Evol 2019 Apr 15. Epub 2019 Apr 15.

Instituto de Biología, Universidad Nacional Autónoma de México, Tercer Circuito s/n de Ciudad Universitaria, Ciudad de México 04510, Mexico.

Biologists energetically debate terminology in ecology and evolution, but rarely discuss general strategies for resolving these debates. We suggest focusing on metaphors, arguing that, rather than looking down on metaphors, biologists should embrace these terms as the powerful tools they are. Like any powerful tool, metaphors need to be used mindful of their limitations. Read More

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http://dx.doi.org/10.1016/j.tree.2019.03.001DOI Listing

Liposomes loaded with phenolic extracts of Spirulina LEB-18: Physicochemical characterization and behavior under simulated gastrointestinal conditions.

Food Res Int 2019 Jun 14;120:656-667. Epub 2018 Nov 14.

Centro de Engenharia Biológica, Universidade do Minho, Campus de Gualtar, Braga 4710-057, Portugal; INL - International Iberian Nanotechnology Laboratory, Av. Mestre José Veiga, Braga 4715-330, Portugal. Electronic address:

Liposomes composed of rice (RL) and soybean (SL) lecithins were produced by reverse phase evaporation and used for the encapsulation of phenolic extracts from Spirulina LEB-18 (S-RL and S-SL for liposomes of rice and soybean lechitin, respectively). Liposomes were characterized in terms of size distribution, polydispersity index, and ζ-potential; the chemical interactions between the phenolic compounds from Spirulina and liposomes were evaluated by Fourier Transform Infrared Spectroscopy (FTIR), and X-ray diffraction (XRD) and differential scanning calorimetry (DSC) were used to evaluate their crystallinity pattern. The release behavior of phenolic extracts was evaluated under two different pH conditions. Read More

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http://dx.doi.org/10.1016/j.foodres.2018.11.023DOI Listing

A thermostable chitinase from the antagonistic Chromobacterium violaceum that inhibits the development of phytopathogenic fungi.

Enzyme Microb Technol 2019 Jul 1;126:50-61. Epub 2019 Apr 1.

Laboratório de Genética Molecular, Departamento de Biologia, Centro de Ciências, UFC, Fortaleza, CE, Brazil. Electronic address:

The biocontrol activity of some soil strains of Chromobacterium sp. against pathogenic fungi has been attributed to secreted chitinases. The aim of this work was to characterize biochemically a recombinant chitinase (CvChi47) from C. Read More

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http://dx.doi.org/10.1016/j.enzmictec.2019.03.009DOI Listing

Targeting host metabolism by inhibition of acetyl-Coenzyme A carboxylase reduces flavivirus infection in mouse models.

Emerg Microbes Infect 2019 ;8(1):624-636

a Department of Biotechnology , Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA) , Madrid , Spain.

Flaviviruses are (re)-emerging RNA viruses strictly dependent on lipid metabolism for infection. In the search for host targeting antivirals, we explored the effect of pharmacological modulation of fatty acid metabolism during flavivirus infection. Considering the central role of acetyl-Coenzyme A carboxylase (ACC) on fatty acid metabolism, we analyzed the effect of three small-molecule ACC inhibitors (PF-05175157, PF-05206574, and PF-06256254) on the infection of medically relevant flaviviruses, namely West Nile virus (WNV), dengue virus, and Zika virus. Read More

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https://www.tandfonline.com/doi/full/10.1080/22221751.2019.1
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http://dx.doi.org/10.1080/22221751.2019.1604084DOI Listing
January 2019
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Extraction of reliable molecular information from diffusion NMR spectroscopy: hydrodynamic volume or molecular mass?

Chemistry 2019 Apr 18. Epub 2019 Apr 18.

Università di Perugia, ITALY.

Measuring accurate translational self-diffusion coefficients (Dt) by NMR with modern spectrometers has become rather routine. On the contrary, the derivation of reliable molecular information therefrom still remains a non-trivial task. In this paper, two established approaches to estimate molecular size in terms of hydrodynamic volume (VH) or molecular weight (MW) are compared. Read More

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http://dx.doi.org/10.1002/chem.201900812DOI Listing

Studying the phosphoryl transfer mechanism of the phosphofructokinase-2: from X-ray structure to quantum mechanics/molecular mechanics simulations.

Chem Sci 2019 Mar 28;10(10):2882-2892. Epub 2019 Jan 28.

Departamento de Biología , Facultad de Ciencias , Universidad de Chile , Santiago , Chile . Email:

Phosphofructokinases (Pfks) catalyze the ATP-dependent phosphorylation of fructose-6-phosphate (F6P) and they are regulated in a wide variety of organisms. Although numerous aspects of the kinetics and regulation have been characterized for Pfks, the knowledge about the mechanism of the phosphoryl transfer reaction and the transition state lags behind. In this work, we describe the X-ray crystal structure of the homodimeric Pfk-2 from , which contains products in one site and reactants in the other, as well as an additional ATP molecule in the inhibitory allosteric site adjacent to the reactants. Read More

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http://dx.doi.org/10.1039/c9sc00094aDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6429617PMC

Self-assembling Releasable Thiocolchicine-Diphenylbutenylaniline Conjugates.

ACS Med Chem Lett 2019 Apr 4;10(4):611-614. Epub 2019 Jan 4.

Dipartimento di Chimica, Università degli Studi di Milano, Via Golgi 19, 20133 Milano, Italy.

The design and the synthesis of new self-assembling conjugates is reported. The target compounds are characterized by the presence of a self-immolative linker that secures a controlled release induced by lipase cleavage. 4-(1,2-Diphenylbut-1-en-1-yl)aniline is used as a self-assembling inducer and amino-thiocolchicine as prototype of drug. Read More

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http://pubs.acs.org/doi/10.1021/acsmedchemlett.8b00605
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http://dx.doi.org/10.1021/acsmedchemlett.8b00605DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6466830PMC
April 2019
1 Read

A Live Vaccine Delivering PcrV through the Type III Secretion System Protects against Pseudomonas aeruginosa.

mSphere 2019 Apr 17;4(2). Epub 2019 Apr 17.

Departamento de Genética, Facultad de Biología, Universidad de Sevilla, Seville, Spain

is a common Gram-negative opportunistic pathogen that is intrinsically resistant to a wide range of antibiotics. The development of a broadly protective vaccine against remains a major challenge. Here, we used an attenuated strain of serovar Typhimurium as a vehicle to express antigens. Read More

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http://dx.doi.org/10.1128/mSphere.00116-19DOI Listing

Synthesis and Characterization of Syndiotactic Polystyrene-Polyethylene Block Copolymer.

Polymers (Basel) 2019 Apr 16;11(4). Epub 2019 Apr 16.

Dipartimento di Chimica e Biologia "Adolfo Zambelli", Università degli Studi di Salerno, Via Giovanni Paolo II, 84084 Fisciano (SA), Italy.

The direct synthesis of syndiotactic polystyrene-block-polyethylene copolymer (sPS--PE) with a diblock structure has been achieved. The synthetic strategy consists of the sequential stereocontrolled polymerization of styrene and ethylene in the presence of a single catalytic system: cyclopentadienyltitanium(IV) trichloride activated by modified methylaluminoxane (CpTiCl/MMAO). The reaction conditions suitable for affording the partially living polymerization of these monomers were identified, and the resulting copolymer, purified from contaminant homopolymers, was fully characterized. Read More

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https://www.mdpi.com/2073-4360/11/4/698
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http://dx.doi.org/10.3390/polym11040698DOI Listing
April 2019
1 Read

Intracellular Peptides in Cell Biology and Pharmacology.

Biomolecules 2019 Apr 16;9(4). Epub 2019 Apr 16.

Department of Pharmacology, Biomedical Sciences Institute, University of São Paulo (USP), São Paulo 05508-000, Brazil.

Intracellular peptides are produced by proteasomes following degradation of nuclear, cytosolic, and mitochondrial proteins, and can be further processed by additional peptidases generating a larger pool of peptides within cells. Thousands of intracellular peptides have been sequenced in plants, yeast, zebrafish, rodents, and in human cells and tissues. Relative levels of intracellular peptides undergo changes in human diseases and also when cells are stimulated, corroborating their biological function. Read More

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https://www.mdpi.com/2218-273X/9/4/150
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http://dx.doi.org/10.3390/biom9040150DOI Listing
April 2019
2 Reads

Hymenoptera Complex Associated with and spp. in Peach Orchards in Northeastern Spain and Prospects for Biological Control of Aphids.

Insects 2019 Apr 16;10(4). Epub 2019 Apr 16.

Institut de Recerca i Tecnologia Agroalimentàries (IRTA), Ctra de Cabrils Km 2, 08348 Cabrils, Barcelona, Spain.

Aphids are a serious pest for peach crops. They have traditionally been managed with insecticides, but there is increasing concern about the risk that insecticides pose to both humans and the environment. As a first step to use biological control in aphid management, we conducted a 3-year field survey in northeastern Spain to determine which parasitoids and hyperparasitoids were most prevalent on two aphids, (Sulzer) and spp. Read More

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http://dx.doi.org/10.3390/insects10040109DOI Listing

Schistosomiasis mansoni in the northeast region of brazil: temporal modeling of positivity, hospitalization,and mortality rates.

Rev Soc Bras Med Trop 2019 Apr 11;52:e20180458. Epub 2019 Apr 11.

Departamento de Medicina, Campus Arapiraca, Universidade Federal de Alagoas, Arapiraca, AL, Brasil.

Introduction: We aimed to analyze trends in Schistosomiasis positivity, mortality, and hospitalization rates in Northeast Brazil.

Methods: We conducted an ecological study using data from the Brazilian Schistosomiasis Control Program, and Hospital and Mortality Information Systems. A joinpoint regression model was used for temporal analysis. Read More

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http://dx.doi.org/10.1590/0037-8682-0458-2018DOI Listing

Increase in the diagnosis of mycobacteremia after the implementation of semi-automated blood culture at a Brazilian public health laboratory.

Rev Soc Bras Med Trop 2019 Apr 11;52:e20180267. Epub 2019 Apr 11.

Departamento de Biologia Médica, Seção de Micobactérias, Laboratório de Saúde Pública Dr. Giovanni Cysneiros, Goiânia, GO, Brasil.

Introduction: The prevalence of hematogenous dissemination of mycobacteria is high in immunosuppressed patients. The isolation of mycobacteria in culture remains the standard procedure.

Methods: This is a cross-sectional study based on the results of solid (Löwenstein-Jensen medium) and semi-automated liquid (BACTEC 9240) blood cultures, obtained from the Lacen-GO database. Read More

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http://dx.doi.org/10.1590/0037-8682-0267-2018DOI Listing

Different susceptibilities of Aedes aegypti and Aedes albopictus larvae to plant-derived products.

Rev Soc Bras Med Trop 2019 Apr 11;52:e20180197. Epub 2019 Apr 11.

Mestrado Acadêmico em Sociobiodiversidade e Tecnologias Sustentáveis, Universidade da Integração Internacional da Lusofonia Afro-Brasileira, Redenção, CE, Brasil.

Introduction: Aedes aegypti and Aedes albopictus are important vectors that transmit arboviruses to human populations.

Methods: Natural products were obtained and tested against larvae collected from the field in Fortaleza, capital of Ceará state.

Results: The essential oils of Syzygium aromaticum (Ae. Read More

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http://dx.doi.org/10.1590/0037-8682-0197-2018DOI Listing

Exploring the Insecticide and Acaricide Potential of Development Regulators obtained from Restinga vegetation from Brazil.

An Acad Bras Cienc 2019 8;91(1):e20180381. Epub 2019 Apr 8.

Laboratório de Estudos de Pragas e Parasitas, Departamento de Biologia Celular e Molecular, Universidade Federal Fluminense, Outeiro de São João Batista, s/n Centro, 24020 140 Niterói, RJ, Brazil.

As a part of our continuing search for insect and arthropod development regulators from Brazilian restinga vegetation of the Rio de Janeiro State, crude extracts, purified fractions and essential oils were submitted to screening tests seeking for biological activities on the development of the insects Rhodnius prolixus, Dysdercus peruvianus, Oncopeltus fasciatus and Rhipicephalus (Boophilus) microplus. Up to now, 102 secondary metabolites have been detected in the fractions, among them monoterpenes, sesquiterpenes and two triterpenes which were obtained from the species, Eugenia sulcata, Pilocarpus spicatus, Manilkara subsericea, Myrciaria floribunda and Zanthoxylum caribaeum. These secondary plant metabolites are considered of interest for the use of studies related to arthropod endocrinology, vector-parasite interaction system, and population control of vector insect and agricultural pest. Read More

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http://dx.doi.org/10.1590/0001-3765201920180381DOI Listing

Redescription of immatures of Dasyhelea flavifrons Guérin-Méneville (Culicomorpha: Ceratopogonidae) and new contribution to the knowledge of its larval habitats.

An Acad Bras Cienc 2019 8;91(1):e20180047. Epub 2019 Apr 8.

Centro de Estudios Parasitológicos y de Vectores/CEPAVE, Facultad de Ciencias Naturales y Museo/UNLP, Consejo Nacional de Investigaciones Científicas y Técnicas/CONICET, Boulevard 120, s/n, e/61 y 62 La Plata, Buenos Aires, Argentina.

The fourth instar larva and pupa of Dasyhelea flavifrons Guérin-Méneville are redescribed, illustrated, and photomicrographed using binocular, phase-contrast, and scanning electron microscopy. Comparisons with the American species of the grisea group were made. The immatures were collected by using a siphon bottle in tree-holes and from water collected in dead snail shells in Salta Province, Argentina, transported to the laboratory and there bred to the emergence of the adults. Read More

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http://dx.doi.org/10.1590/0001-3765201920180047DOI Listing

Health benefits of subcutaneous zinc edetate and diphenyl diselenide in calves during the weaning period.

An Acad Bras Cienc 2019 8;91(1):e20171042. Epub 2019 Apr 8.

Departamento de Zootecnia, Universidade do Estado de Santa Catarina/UDESC, Rua Beloni Trombeta Zanin, 680D, 89815-630 Chapecó, SC, Brazil.

The aims of this study were to investigate whether treatments with zinc edetate (Zn) and diphenyl diselenide ((PhSe)2) enhance performance, immune responses, protein metabolism, and oxidant/antioxidant status in calf serum and muscle. Animals were divided into four groups (n=6 each): control (without supplementation), and groups supplemented on days 50 and 70 of life with (PhSe)2, Zn, and a combination of (PhSe)2 and Zn. Animals treated with (PhSe)2 gained more weight by experimental day 220 than did the control group, but there was no difference by the end of the experiment (day 300). Read More

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http://dx.doi.org/10.1590/0001-3765201920171042DOI Listing

Ecological aspects of natural populations of Hyalella pampeana (Crustacea, Amphipoda, Hyalellidae) from the Natural Reserve Island of Martín García (Río de La Plata, Argentina).

An Acad Bras Cienc 2019 8;91(1):e20170928. Epub 2019 Apr 8.

Departamento de Biología, Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Mar del Plata/FCEyN, UNMdP, Funes St. 3350, 7600 Mar del Plata, Argentina.

Freshwater amphipods of the genus Hyalella have a great importance in aquatic ecosystems due to their role in matter and energy cycling and its utilization as bioindicators of environmental health. The aim of this work was to analyze relevant population parameters of Hyalellapampeana, such as population density and structure, individual body size, sex ratio, fecundity and recruitment, and to study the possible relation of these parameters to variations in environmental variables. Samples were taken monthly during a one-year period (2006) at three small ponds in the Natural Reserve Island of Martín García, Argentina. Read More

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http://dx.doi.org/10.1590/0001-3765201920170928DOI Listing

Daily Variations in the Expression of Genes Related to Insecticide Resistance in the Chagas Disease Vector (Hemiptera: Reduviidae).

Am J Trop Med Hyg 2019 Apr 15. Epub 2019 Apr 15.

Instituto de Investigaciones en Ciencias de la Salud (INICSA), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Cátedra de Bioquímica y Biología Molecular, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, Córdoba, Argentina.

Pyrethroid resistance has been detected in , which in part has been attributed to increased oxidative metabolism of the insecticide by cytochrome P450s. Nicotinamide adenine dinucleotide phosphate (NADPH) cytochrome P450 reductase (CPR) catalyzes electron transfer from NADPH to all known cytochrome P450s. In this study, the expression of the gene at transcriptional level was determined in different tissues and two life stages. Read More

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http://dx.doi.org/10.4269/ajtmh.19-0155DOI Listing

Deciphering the number and location of active sites in the monomeric glyoxalase I of Zea mays.

FEBS J 2019 Apr 16. Epub 2019 Apr 16.

Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI-CONICET), Universidad Nacional de Rosario, Suipacha 531, S2002LRK, Rosario, Argentina.

Detoxification of methylglyoxal, a toxic by-product of central sugar metabolism, is a major issue for all forms of life. The glyoxalase pathway evolved to effectively convert methylglyoxal into D-lactate via a glutathione hemithioacetal intermediate. Recently, we have shown that the monomeric glyoxalase I from maize exhibits a symmetric fold with two cavities, potentially harboring two active sites, in analogy with homodimeric enzyme surrogates. Read More

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http://dx.doi.org/10.1111/febs.14855DOI Listing
April 2019
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Complete assembly of the Leishmania donovani (HU3 strain) genome and transcriptome annotation.

Sci Rep 2019 Apr 16;9(1):6127. Epub 2019 Apr 16.

Centro de Biología Molecular "Severo Ochoa" (CSIC/UAM), Campus de Excelencia Internacional (CEI) UAM+CSIC, Universidad Autónoma de Madrid, Madrid, Spain.

Leishmania donovani is a unicellular parasite that causes visceral leishmaniasis, a fatal disease in humans. In this study, a complete assembly of the genome of L. donovani is provided. Read More

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http://dx.doi.org/10.1038/s41598-019-42511-4DOI Listing

New species need characters: comments on recently described apicomplexan parasites from Australia.

Authors:
D James Harris

Parasit Vectors 2019 Apr 16;12(1):172. Epub 2019 Apr 16.

Centro de Investigação em Biodiversidade e Recursos Genéticos (CIBIO), InBIO Laboratório Associado, Universidade do Porto, Campus Agrário de Vairão, 4485-661, Vairão, Portugal.

Screening of ticks from companion animals from Australia using genetic tools has recently been used to identify and name eight new species of Apicomplexan parasites. However, the diagnosis of these was based on genetic distinctiveness and phylogenetic relationships rather than determination of specific characters, which is required by the International Code of Zoological Nomenclature. The recently proposed names are therefore invalid. Read More

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https://parasitesandvectors.biomedcentral.com/articles/10.11
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http://dx.doi.org/10.1186/s13071-019-3424-9DOI Listing
April 2019
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Manipulation of oxidative stress responses as a strategy to generate stress-tolerant crops. From damage to signaling to tolerance.

Crit Rev Biotechnol 2019 Apr 16:1-16. Epub 2019 Apr 16.

a Facultad de Ciencias Bioquímicas y Farmacéuticas, Biología del Estrés en Plantas, Instituto de Biología Molecular y Celular de Rosario (IBR-UNR/CONICET) , Universidad Nacional de Rosario (UNR) , Rosario , Argentina.

Plants exposed to hostile environmental conditions such as drought or extreme temperatures usually undergo oxidative stress, which has long been assumed to significantly contribute to the damage suffered by the organism. Reactive oxygen species (ROS) overproduced under stress conditions were proposed to destroy membrane lipids and to inactivate proteins and photosystems, ultimately leading to cell death. Accordingly, considerable effort has been devoted, over the years, to improve stress tolerance by strengthening antioxidant and dissipative mechanisms. Read More

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https://www.tandfonline.com/doi/full/10.1080/07388551.2019.1
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http://dx.doi.org/10.1080/07388551.2019.1597829DOI Listing
April 2019
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Amputation Risk Factors in Severely Frostbitten Patients.

Int J Environ Res Public Health 2019 Apr 15;16(8). Epub 2019 Apr 15.

Secció de Fisiologia, Departament de Biologia Cel·lular, Fisiologia i Immunologia, Facultat de Biologia, Universitat de Barcelona, 08028 Barcelona, Spain.

In recent years, the incidence of frostbite has increased among healthy young adults who practice winter sports (skiing, mountaineering, ice climbing and technical climbing/alpinism) at both the professional and amateur levels. Moreover, given that the population most frequently affected is healthy and active, frostbite supposes a substantial interruption of their normal activity and in most cases is associated with long-term sequelae. It particularly has a higher impact when the affected person's daily activities require exposure to cold environments, as either sports practices or work activities in which low temperatures are a constant (ski patrols, mountain guides, avalanche forecasters, workers in the cold chain, etc. Read More

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https://www.mdpi.com/1660-4601/16/8/1351
Publisher Site
http://dx.doi.org/10.3390/ijerph16081351DOI Listing
April 2019
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Chokeberry Juice Containing Polyphenols Does Not Affect Cholesterol or Blood Pressure but Modifies the Composition of Plasma Phospholipids Fatty Acids in Individuals at Cardiovascular Risk.

Nutrients 2019 Apr 15;11(4). Epub 2019 Apr 15.

Center of Research Excellence in Nutrition and Metabolism, Institute for Medical Research, University of Belgrade, 11000 Belgrade, Serbia.

Chokeberry polyphenols have been suggested to reduce cholesterol and blood pressure and thus protect against cardiovascular diseases (CVD), but the evidence in humans is limited and inconsistent. This randomized double-blinded three-parallel groups trial investigated the changes in various anthropometric and clinical biomarkers, and in plasma phospholipids fatty acids (PPFA) in volunteers at cardiovascular risk after a four-week intervention with 100 mL/day of (1) chokeberry juice with a high-dose of polyphenols (1177.11 mg gallic acid equivalents, GAE); (2) chokeberry juice with a low-dose of polyphenols (294. Read More

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http://dx.doi.org/10.3390/nu11040850DOI Listing

Comparative proteomic analysis of saliva from dogs with and without obesity-related metabolic dysfuntion.

J Proteomics 2019 Apr 13. Epub 2019 Apr 13.

Interlab-UMU, Regional Campus of International Excellence Campus Mare Nostrum, University of Murcia, Murcia 30100, Spain. Electronic address:

Dogs develop only some of the components of the human metabolic syndrome (MetS). Thus, in order to study possible MetS-related alterations in dogs, human MetS criteria were adapted to define canine MetS or so-called obesity-related metabolic dysfunction (ORMD). The main objective of this study was to identify changes in the salivary proteome of obese dogs with ORMD in comparison with obese dogs without ORMD which may constitute potential salivary biomarkers for assessing ORMD. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S18743919193011
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http://dx.doi.org/10.1016/j.jprot.2019.04.010DOI Listing
April 2019
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