324,014 results match your criteria Bioinformatics Oxford England[Journal]


Inflammatory pathway analytes predicting rapid cognitive decline in MCI stage of Alzheimer's disease.

Ann Clin Transl Neurol 2020 Jul 7. Epub 2020 Jul 7.

Lou Ruvo Center for Brain Health, Cleveland Clinic, Cleveland, Ohio, 44195.

Objective: To determine the inflammatory analytes that predict clinical progression and evaluate their performance against biomarkers of neurodegeneration.

Methods: A longitudinal study of MCI-AD patients in a Discovery cohort over 15 months, with replication in the Alzheimer's Disease Neuroimaging Initiative (ADNI) MCI cohort over 36 months. Fifty-three inflammatory analytes were measured in the CSF and plasma with a RBM multiplex analyte platform. Read More

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http://dx.doi.org/10.1002/acn3.51109DOI Listing

Effect of Haemophilus influenzae type b and 13-valent pneumococcal conjugate vaccines on childhood pneumonia hospitalizations and deaths in Botswana.

Clin Infect Dis 2020 Jul 8. Epub 2020 Jul 8.

Division of Pediatric Infectious Diseases, Duke University, Durham, NC.

Background: Globally, pneumonia is the leading cause of death among children. Few data exist regarding the effect of Haemophilus influenzae type b (Hib) vaccine and 13-valent pneumococcal conjugate vaccine (PCV-13) on the burden of childhood pneumonia in African settings.

Methods: We collected data on children 1 to 59 months of age at three hospitals in Botswana. Read More

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http://dx.doi.org/10.1093/cid/ciaa919DOI Listing

A detailed in silico analysis of the amylolytic family GH126 and its possible relatedness to family GH76.

Carbohydr Res 2020 Jun 23;494:108082. Epub 2020 Jun 23.

Laboratory of Protein Evolution, Institute of Molecular Biology, Slovak Academy of Sciences, SK-84551, Bratislava, Slovakia; Department of Biology, Faculty of Natural Sciences, University of SS. Cyril and Methodius, SK-91701, Trnava, Slovakia. Electronic address:

The glycoside hydrolase (GH) family 126 was established based on the X-ray structure determination of the amylolytic enzyme CPF_2247 from Clostridium perfringens genome. Its original identification as a putative carbohydrate-active enzyme was based on its low, yet significant sequence identity to members of the family GH8, which are inverting endo-β-1,4-glucanases. As the family GH8 forms the clan GH-M with GH48, the CPF_2247 protein also exhibits similarities with members of the family GH48. Read More

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http://dx.doi.org/10.1016/j.carres.2020.108082DOI Listing

Evolution of the mutation rate across primates.

Curr Opin Genet Dev 2020 Jul 4;62:58-64. Epub 2020 Jul 4.

Department of Molecular and Cell Biology, University of California, Berkeley, CA, United States; Center for Computational Biology, University of California, Berkeley, CA, United States. Electronic address:

Germline mutations are the source of all heritable variation. In the past few years, whole genome sequencing has allowed direct and comprehensive surveys of mutation patterns in humans and other species. These studies have documented substantial variation in both mutation rates and spectra across primates, the causes of which remain unclear. Read More

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http://dx.doi.org/10.1016/j.gde.2020.05.028DOI Listing

Metal induced non-metallothionein protein in earthworm: A new pathway for cadmium detoxification in chloragogenous tissue.

J Hazard Mater 2020 Jul 1;401:123357. Epub 2020 Jul 1.

Soil and Agro Bio-engineering Lab, Department of Environmental Science, Tezpur University, Tezpur, 784028, India. Electronic address:

Earthworms neutralize toxic metals by a small (∼13 kDa) cysteine rich metal binding protein, metallothionein (MT). Although the rate of metal accumulation and MT expression does not correlate well, the reason behind such inconsistency has not yet been deciphered. The present investigation clearly demonstrates that expression of some non-MT metal induced proteins is responsible for such incongruity. Read More

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http://dx.doi.org/10.1016/j.jhazmat.2020.123357DOI Listing

Emerging bacterial fish pathogen Lactococcus garvieae RTCLI04, isolated from rainbow trout (Oncorhynchus mykiss): Genomic features and comparative genomics.

Microb Pathog 2020 Jul 4:104368. Epub 2020 Jul 4.

ICAR-Directorate of Coldwater Fisheries Research, Bhimtal, 263 136, Nainital, Uttarakhand, India.

Lactococcus garvieae is one of the emerging zoonotic bacterial pathogen, causes fatal hemorrhagic septicemia in cultured fish species, animals and humans, worldwide. Here, we report the genomic features of whole-genome sequence (WGS) of L. garvieae strain RTCLI04, recovered from lower intestine of farmed rainbow trout, Oncorhynchus mykiss in the northwest Himalayan region India. Read More

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http://dx.doi.org/10.1016/j.micpath.2020.104368DOI Listing

Should Short-course Neoadjuvant Radiotherapy Be Applied for Low-lying Rectal Cancer? A Systematic Review and Meta-analysis of the Randomized Trials.

Int J Radiat Oncol Biol Phys 2020 Jul 4. Epub 2020 Jul 4.

Department of Radiotherapy I, Maria Sklodowska-Curie National Research Institute of Oncology, Warsaw, Poland.

Background: National Comprehensive Cancer Network guidelines recommend either long-course chemoradiation (LC) or short-course radiation (SC, 5 × 5 Gy) for rectal cancer before total mesorectal excision. However, they do not recommend SC for low-lying tumors. As early toxicity of SC is lower than that of LC, and postoperative complications as well as late toxicity are similar, the probable reason is a notion that for low-lying tumors LC may be more effective than SC in assuring local control. Read More

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http://dx.doi.org/10.1016/j.ijrobp.2020.06.077DOI Listing

Physalin A attenuates inflammation through down-regulating c-Jun NH2 kinase phosphorylation/Activator Protein 1 activation and up-regulating the antioxidant activity.

Toxicol Appl Pharmacol 2020 Jul 4:115115. Epub 2020 Jul 4.

Department of Pharmacology, China Medical University, Taichung 40402, Taiwan, ROC; Chinese Medicine Research Center, China Medical University, Taichung 40402, Taiwan, ROC; Drug Development Center, China Medical University, Taichung 40402, Taiwan, ROC. Electronic address:

Physalin A (PA), a withanolide, was isolated from Physalis angulata L. In this study, it is shown that PA can inhibit the production of inflammatory cytokines such as PGE, NO, IL-1β, IL-6, and TNF-α in LPS-induced RAW 264.7 cells. Read More

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http://dx.doi.org/10.1016/j.taap.2020.115115DOI Listing

Update on therapeutic approaches and emerging therapies for SARS-CoV-2 virus.

Eur J Pharmacol 2020 Jul 4:173348. Epub 2020 Jul 4.

United States International University-Africa, School of Pharmacy and Health Sciences, Department of Pharmaceutics, P. O. Box 14634-00800, Nairobi, Kenya. Electronic address:

The global pandemic of coronavirus disease 2019 (COVID-19), caused by novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has resulted in over 7,273,958 cases with almost over 413,372 deaths worldwide as per the WHO situational report 143 on COVID-19. There are no known treatment regimens with proven efficacy and vaccines thus far, posing an unprecedented challenge to identify effective drugs and vaccines for prevention and treatment. The urgency for its prevention and cure has resulted in an increased number of proposed treatment options. Read More

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http://dx.doi.org/10.1016/j.ejphar.2020.173348DOI Listing

A Single-Cell Transcriptomics CRISPR-Activation Screen Identifies Epigenetic Regulators of the Zygotic Genome Activation Program.

Cell Syst 2020 Jun 29. Epub 2020 Jun 29.

Epigenetics Programme, Babraham Institute, Cambridge CB22 3AT, UK; Wellcome Trust Sanger Institute, Hinxton, Cambridge CB10 1SA, UK; Centre for Trophoblast Research University of Cambridge, Cambridge CB2 3EG, UK. Electronic address:

Zygotic genome activation (ZGA) is an essential transcriptional event in embryonic development that coincides with extensive epigenetic reprogramming. Complex manipulation techniques and maternal stores of proteins preclude large-scale functional screens for ZGA regulators within early embryos. Here, we combined pooled CRISPR activation (CRISPRa) with single-cell transcriptomics to identify regulators of ZGA-like transcription in mouse embryonic stem cells, which serve as a tractable, in vitro proxy of early mouse embryos. Read More

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http://dx.doi.org/10.1016/j.cels.2020.06.004DOI Listing

Effects of malleable kinetochore morphology on measurements of intrakinetochore tension.

Open Biol 2020 Jul 8;10(7):200101. Epub 2020 Jul 8.

New York State Department of Health, Wadsworth Center, Albany, NY, USA.

The distance between fluorescent spots formed by various kinetochore proteins (delta) is commonly interpreted as a manifestation of intrakinetochore tension (IKT) caused by microtubule-mediated forces. However, large-scale changes of the kinetochore architecture (such as its shape or dimensions) may also contribute to the value of delta. To assess contributions of these non-elastic changes, we compare behaviour of delta values in human kinetochores with small yet mechanically malleable kinetochores against compound kinetochores in Indian muntjac (IM) cells whose architecture remains constant. Read More

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http://dx.doi.org/10.1098/rsob.200101DOI Listing

ADAMTS13 and VWF activities guide individualized caplacizumab treatment in patients with aTTP.

Blood Adv 2020 Jul;4(13):3093-3101

Department of Nephrology and Hypertension, Medical School Hannover, Hannover, Germany.

Introduction of the nanobody caplacizumab was shown to be effective in the treatment of acquired thrombotic thrombocytopenic purpura (aTTP) in the acute setting. The official recommendations include plasma exchange (PEX), immunosuppression, and the use of caplacizumab for a minimum of 30 days after stopping daily PEX. This study was a retrospective, observational analysis of the use of caplacizumab in 60 patients from 29 medical centers in Germany. Read More

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http://dx.doi.org/10.1182/bloodadvances.2020001987DOI Listing

Real-world data confirm the effectiveness of caplacizumab in acquired thrombotic thrombocytopenic purpura.

Blood Adv 2020 Jul;4(13):3085-3092

Department of Nephrology and Hypertension, Medical School Hannover, Hannover, Germany.

Acquired thrombotic thrombocytopenic purpura (aTTP) is a rare but life-threatening condition. In 2018, the nanobody caplacizumab was approved for the treatment of adults experiencing an acute episode of aTTP, in conjunction with plasma exchange (PEX) and immunosuppression for a minimum of 30 days after stopping daily PEX. We performed a retrospective, observational analysis on the use of caplacizumab in 60 patients from 29 medical centers in Germany during acute disease management. Read More

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http://dx.doi.org/10.1182/bloodadvances.2020001973DOI Listing

Targeted sequencing of genes associated with the mismatch repair pathway in patients with endometrial cancer.

PLoS One 2020 7;15(7):e0235613. Epub 2020 Jul 7.

Department of Medical Genetics, St. Olavs Hospital, Trondheim, Norway.

Germline variants inactivating the mismatch repair (MMR) genes MLH1, MSH2, MSH6 and PMS2 cause Lynch syndrome that implies an increased cancer risk, where colon and endometrial cancer are the most frequent. Identification of these pathogenic variants is important to identify endometrial cancer patients with inherited increased risk of new cancers, in order to offer them lifesaving surveillance. However, several other genes are also part of the MMR pathway. Read More

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0235613PLOS

Skeletal muscle dysregulation in rheumatoid arthritis: Metabolic and molecular markers in a rodent model and patients.

PLoS One 2020 7;15(7):e0235702. Epub 2020 Jul 7.

Division of Physiology, Pharmacology and Neuroscience, School of Life Science, University of Nottingham, Nottingham, England, United Kingdom.

Rheumatoid arthritis (RA) is accompanied by pain, inflammation and muscle weakness. Skeletal muscle inflammation and inactivity are independently associated with muscle insulin resistance and atrophy. Our objective was to identify early molecular and biochemical markers in muscle from a rodent model of RA relative to control and subsequently identify commonality in muscle gene expression between this model and muscle from RA patients. Read More

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0235702PLOS

Transcriptional frameshifts contribute to protein allergenicity.

J Clin Invest 2020 Jul 7. Epub 2020 Jul 7.

Genclis SA, Vandoeuvre-lès-Nancy, France.

Transcription infidelity (TI) is a mechanism that increases RNA and protein diversity. We found that single-base omissions (i.e. Read More

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http://dx.doi.org/10.1172/JCI126275DOI Listing

Bioinformatics analysis of high-throughput data to validate potential novel biomarkers and small molecule drugs for glioblastoma multiforme.

J Int Med Res 2020 Jul;48(7):300060520924541

The First People's Hospital of Taicang City, Taicang Affiliated Hospital of Soochow University, Suzhou, P. R. China.

Objective: There have been no recent improvements in the glioblastoma multiforme (GBM) outcome, with median survival remaining 15 months. Consequently, the need to identify novel biomarkers for GBM diagnosis and prognosis, and to develop targeted therapies is high. This study aimed to establish biomarkers for GBM pathogenesis and prognosis. Read More

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http://dx.doi.org/10.1177/0300060520924541DOI Listing

Identification of hub genes involved in apparent metabolizable energy of chickens.

Anim Biotechnol 2020 Jul 7:1-8. Epub 2020 Jul 7.

Department of Animal Sciences, Faculty of Agriculture, University of Guilan, Rasht, Iran.

Feed efficiency is one of the most economically significant traits in a breeding program. Apparent metabolizable energy is the most used method to evaluate energy utilization for feed efficiency. The purpose of this study was to identify candidate genes of chickens with divergent apparent metabolizable energy by bioinformatics analysis. Read More

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http://dx.doi.org/10.1080/10495398.2020.1784187DOI Listing

Comparative phylogenetic analysis of and with other members of subfamilies of cyprinidae on the basis of complete mitochondrial genome and 12S, 16S ribosomal RNA from Northren areas of Pakistan.

Mitochondrial DNA A DNA Mapp Seq Anal 2020 Jul 7:1-7. Epub 2020 Jul 7.

Department of Bioinformatics, Hazara University, Mansehra, Pakistan.

We assessed the relationship of Schizothoracinae species with other subfamilies Alburninae, Xenocyprinae, Cultrinae and Squaliobarbinae of family Cyprinidae by creating the phylogenetic trees using complete mitogenome and 12S and 16S RNA. Our representative species show the great affiliation with other but separated from a group composed of , , and of subfamily Alburninae while other subfamilies formed distinct group. The members of subfamily Schizothoracinae shows separate line of evolution from subfamily Barbinae. Read More

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http://dx.doi.org/10.1080/24701394.2020.1787397DOI Listing

Discovery of Novel Small-Molecule Calcium Sensitizers for Cardiac Troponin C: A Combined Virtual and Experimental Screening Approach.

J Chem Inf Model 2020 Jul 7. Epub 2020 Jul 7.

Department of Chemistry and Biochemistry, Ohio State University, Columbus, Ohio 43210, United States.

Heart failure is a leading cause of death throughout the world and is triggered by a disruption of the cardiac contractile machinery. This machinery is regulated in a calcium-dependent manner by the protein complex troponin. Calcium binds to the N-terminal domain of cardiac troponin C (cNTnC) setting into motion the cascade of events leading to muscle contraction. Read More

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http://dx.doi.org/10.1021/acs.jcim.0c00452DOI Listing

Cell-free DNA profiling in retinoblastoma patients with advanced intraocular disease: An MSKCC experience.

Cancer Med 2020 Jul 7. Epub 2020 Jul 7.

Memorial Sloan Kettering Cancer Center, New York, NY, USA.

Purpose: The enucleation rate for retinoblastoma has dropped from over 95% to under 10% in the past 10 years as a result of improvements in therapy. This reduces access to tumor tissue for molecular profiling, especially in unilateral retinoblastoma, and hinders the confirmation of somatic RB1 mutations necessary for genetic counseling. Plasma cell-free DNA (cfDNA) has provided a platform for noninvasive molecular profiling in cancer, but its applicability in low tumor burden retinoblastoma has not been shown. Read More

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http://dx.doi.org/10.1002/cam4.3144DOI Listing

Overcoming vincristine resistance in cancer: computational design and discovery of piperine inspired P-glycoprotein inhibitors.

Chem Biol Drug Des 2020 Jul 7. Epub 2020 Jul 7.

Centre for Bioinformatics, School of Life Sciences, Pondicherry University, Kalapet, Puducherry- 605014, India.

P-glycoprotein (P-gp)/MDR-1 plays a major role in the development of multidrug resistance (MDR) by pumping the chemotherapeutic drugs out of the cancer cells and reducing their efficacy. A number of P-gp inhibitors were reported to reverse the MDR when co-administered with chemotherapeutic drugs. Unfortunately, none has approved for clinical use due to toxicity issues. Read More

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http://dx.doi.org/10.1111/cbdd.13758DOI Listing

Signs and symptoms to determine if a patient presenting in primary care or hospital outpatient settings has COVID-19 disease.

Cochrane Database Syst Rev 2020 Jul 7;7:CD013665. Epub 2020 Jul 7.

NIHR Birmingham Biomedical Research Centre, University Hospitals Birmingham NHS Foundation Trust and University of Birmingham, Birmingham, UK.

Background: Some people with SARS-CoV-2 infection remain asymptomatic, whilst in others the infection can cause mild to moderate COVID-19 disease and COVID-19 pneumonia, leading some patients to require intensive care support and, in some cases, to death, especially in older adults. Symptoms such as fever or cough, and signs such as oxygen saturation or lung auscultation findings, are the first and most readily available diagnostic information. Such information could be used to either rule out COVID-19 disease, or select patients for further diagnostic testing. Read More

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http://dx.doi.org/10.1002/14651858.CD013665DOI Listing

Molecular adaptation and convergent evolution of frugivory in Old World and neotropical fruit bats.

Mol Ecol 2020 Jul 7. Epub 2020 Jul 7.

Department of Ecology, Tibetan Centre for Ecology and Conservation at WHU-TU, Hubei Key Laboratory of Cell Homeostasis, College of Life Sciences, Wuhan University, Wuhan, 430072, China.

Although cases of independent adaptation to the same dietary niche have been documented in mammalian ecology, the molecular correlates of such shifts are seldom known. Here we used genome-wide analyses of molecular evolution to examine two lineages of bats that, from an insectivorous ancestor, have both independently evolved obligate frugivory: the Old World family Pteropodidae and the neotropical subfamily Stenodermatinae. New genome assemblies from two neotropical fruit bats (Artibeus jamaicensis and Sturnira hondurensis) provide a framework for comparisons with Old World fruit bats. Read More

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http://dx.doi.org/10.1111/mec.15542DOI Listing

Taxonomic classification method for metagenomics based on core protein families with Core-Kaiju.

Nucleic Acids Res 2020 Jul 7. Epub 2020 Jul 7.

Physics and Astronomy Department, LIPh Lab, University of Padova, Via Marzolo 8, 35131 Padova, Italy.

Characterizing species diversity and composition of bacteria hosted by biota is revolutionizing our understanding of the role of symbiotic interactions in ecosystems. Determining microbiomes diversity implies the assignment of individual reads to taxa by comparison to reference databases. Although computational methods aimed at identifying the microbe(s) taxa are available, it is well known that inferences using different methods can vary widely depending on various biases. Read More

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http://dx.doi.org/10.1093/nar/gkaa568DOI Listing

A mechanistic model and therapeutic interventions for COVID-19 involving a RAS-mediated bradykinin storm.

Elife 2020 Jul 7;9. Epub 2020 Jul 7.

Biosciences, Oak Ridge National Laboratory, Oak Ridge, United States.

Neither the disease mechanism nor treatments for COVID-19 are currently known. Here we present a novel molecular mechanism for COVID-19 that provides therapeutic intervention points that can be addressed with existing FDA-approved pharmaceuticals. The entry point for the virus is ACE2, which is a component of the counteracting hypotensive axis of RAS, that produces the nonapeptide angiotensin from angiotensin I. Read More

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http://dx.doi.org/10.7554/eLife.59177DOI Listing

Dopamine role in learning and action inference.

Authors:
Rafal Bogacz

Elife 2020 Jul 7;9. Epub 2020 Jul 7.

Medical Research Council Brain Network Dynamics Unit, University of Oxford, Oxford, United Kingdom.

This paper describes a framework for modelling dopamine function in the mammalian brain. It proposes that both learning and action planning involve processes minimizing prediction errors encoded by dopaminergic neurons. In this framework, dopaminergic neurons projecting to different parts of the striatum encode errors in predictions made by the corresponding systems within the basal ganglia. Read More

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http://dx.doi.org/10.7554/eLife.53262DOI Listing

The influence of the gastrointestinal microbiome on infant colic.

Expert Rev Gastroenterol Hepatol 2020 Jul 7. Epub 2020 Jul 7.

KidZ Health Castle, Universitair Ziekenhuis Brussel, Vrije Universiteit Brussel , Brussels, Belgium.

Introduction: Although infantile colic is relatively frequent, its pathophysiology is not yet understood. The aim of this paper is to provide a better understanding of the link between infantile colic and the gastro-intestinal microbiome.

Areas Covered: The gastro-intestinal microbiome may already start to develop in the womb and grows exponentially immediately after birth. Read More

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http://dx.doi.org/10.1080/17474124.2020.1791702DOI Listing

Exosomes derived from mmu_circ_0000623-modified ADSCs prevent liver fibrosis via activating autophagy.

Authors:
M Zhu X Liu W Li L Wang

Hum Exp Toxicol 2020 Jul 7:960327120931152. Epub 2020 Jul 7.

Geriatric Department, Jining No. 1 People's Hospital, Jining, Shandong, China.

Prolonged parenchymal cell death leads to activation of fibrogenic cells, extracellular matrix accumulation, and eventually liver fibrosis. Increasing evidence shows that exosomes (Exos) secreted by adipose-derived mesenchymal stem cells (ADSCs) can be used to deliver circular RNAs (circRNAs) to treat liver fibrosis. To explore the uses of circRNA, circRNA expression profiles of hepatic tissue from normal and CCl-induced mice were analyzed using high-throughput circRNA microarrays. Read More

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http://dx.doi.org/10.1177/0960327120931152DOI Listing

Association of job stress, CLOCK gene polymorphism and their interaction with poor sleep quality.

J Sleep Res 2020 Jul 7:e13133. Epub 2020 Jul 7.

CAS Key Laboratory of Mental Healthy, Institute of Psychology, Chinese Academy of Sciences, Beijing, China.

Job stress and the Circadian Locomotor Output Cycles Kaput (CLOCK) gene could affect circadian rhythm and sleep quality. The main aim of our present study was to investigate the association of job stress, CLOCK gene polymorphism and their interaction with sleep quality in a non-clinical Chinese Han population, which has not been reported to date. Using a cross-sectional design, 450 subjects were recruited in Beijing. Read More

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http://dx.doi.org/10.1111/jsr.13133DOI Listing

LncRNA SNHG15 promotes the proliferation of nasopharyngeal carcinoma via sponging miR-141-3p to upregulate KLF9.

Eur Rev Med Pharmacol Sci 2020 Jun;24(12):6744-6751

Department of Oncology and Hematology, People's Hospital of Leshan, Leshan, China.

Objective: Long non-coding RNAs (lncRNAs) have been identified to exert an oncogenic or anti-tumor function in malignant tumors. LncRNA SNHG15 is verified to be an oncogene in hepatocellular carcinoma, colorectal cancer, and prostate cancer. In this paper, we mainly investigate the potential influence of SNHG15 on the progression of nasopharyngeal carcinoma (NPC). Read More

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http://dx.doi.org/10.26355/eurrev_202006_21662DOI Listing

MiR-769-5p functions as an oncogene by down-regulating RYBP expression in gastric cancer.

Eur Rev Med Pharmacol Sci 2020 Jun;24(12):6699-6706

Department of General Surgery, Yantaishan Hospital, Yantai, China.

Objective: The purpose of this study was to detect the relative expression level of micro-ribonucleic acid (miR)-769-5p in gastric cancer (GC) tissues and cells, and to investigate the clinical significance, biological function, and mechanism of miR-769-5p in GC.

Patients And Methods: The relative expression level in 62 cases of GC tissues and paracancerous tissues was detected by quantitative Real Time-Polymerase Chain Reaction (qRT-PCR). The correlation between miR-769-5p expression and clinicopathological characteristics of GC patients was analyzed by Chi-square test. Read More

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http://dx.doi.org/10.26355/eurrev_202006_21657DOI Listing

Long noncoding RNA SLC30A10 promotes colorectal tumor proliferation and migration via miR-21c/APC axis.

Authors:
L Hou P Liu T Zhu

Eur Rev Med Pharmacol Sci 2020 Jun;24(12):6682-6691

Department of Gastrointestinal Surgery, Affiliated Hospital of Chengde Medical College, Chengde, China.

Objective: Colorectal cancer is a common malignancy and a common cause of tumor-related death. Long non-coding RNAs (lncRNAs) have become an important regulatory factor and tissue specific biomarker for a variety of cancers, including colorectal cancer. Recent evidence indicates that the novel lncRNA SLC30A10 plays an important role in tumor progression and metastasis. Read More

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http://dx.doi.org/10.26355/eurrev_202006_21655DOI Listing

MiR-613 blocked the progression of cervical cancer by targeting LETM1.

Authors:
H Ji N-J Hu

Eur Rev Med Pharmacol Sci 2020 Jun;24(12):6576-6582

Department of Obstetrics and Gynecology, The First Affiliated Hospital of Jinzhou Medical University, Jinzhou, China.

Objective: The purpose of this study was to investigate the potential effects of miR-613 on the development of cervical squamous cell cancer (CSCC) and the relevant mechanism.

Patients And Methods: The expression level of miR-613 was detected in CSCC tissues and cells by quantitative Real Time-Polymerase Chain Reaction (qRT-PCR). Thereafter, online prediction software and Luciferase reporter assays were used to evaluate the possible target of miR-613, and the effects of the miR-613 on SiHa cells were determined in vitro. Read More

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http://dx.doi.org/10.26355/eurrev_202006_21642DOI Listing

Functional movements of the GABA type A receptor.

Phys Chem Chem Phys 2020 Jul 7. Epub 2020 Jul 7.

Bioinformatique Structurale, Institut Pasteur CNRS URA 3528, CB3I CNRS USR 3756, 75724 Paris, France.

We have performed a parallel tempering crankshaft motion Monte Carlo simulation on a model of the GABA type A receptor with the aim of exploring a wide variety of local conformational space. We develop a novel method to analyse the protein movements in terms of a correlation tensor and use this to explore the gating process, that is, how agonist binding could cause ion channel opening. We find that simulated binding impulses to varying clusters of GABA binding site residues produce channel opening, and that equivalent impulses to single GABA sites produce partial opening. Read More

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http://dx.doi.org/10.1039/d0cp01128bDOI Listing

Dipeptidyl Peptidase-4 Inhibitor treatment could decrease Pneumonia in patients with type 2 diabetes.

Postgrad Med 2020 Jul 7. Epub 2020 Jul 7.

Graduate Institute of Biomedical Sciences, China Medical University , Taichung, Taiwan.

Objectives: To investigate the effect of dipeptidyl peptidase-4 inhibitor (DPP4i) for (KP) pneumonia in patients with diabetes.

Patients And Methods: Patients newly diagnosed with type 2 diabetes from 2009 to 2012 were recruited for this population-based and observational study. Diabetes complications severity index (DCSI) score and defined daily dose (DDD) were used for analysis. Read More

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http://dx.doi.org/10.1080/00325481.2020.1793520DOI Listing

MiR-663a, regulated by lncRNA GAS5, contributes to osteosarcoma development through targeting MYL9.

Authors:
S Zhao W Xiong K Xu

Hum Exp Toxicol 2020 Jul 7:960327120937330. Epub 2020 Jul 7.

Department of Orthopaedics, Ningbo Hwa Mei Hospital, University of Chinese Academy of Sciences, Ningbo, Zhejiang, China.

Osteosarcoma is characterized by high malignancy and high metastasis rate, resulting in high mortality and disability. MiR-663a has been reported in a variety of tumors to promote tumorigenesis. However, miR-663a has not been reported in the pathogenesis of osteosarcoma. Read More

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http://dx.doi.org/10.1177/0960327120937330DOI Listing

Robust FCS parsing - Exploring 211359 public files.

Cytometry A 2020 Jul 6. Epub 2020 Jul 6.

Laboratory Medical immunology, Department of Immunology, Erasmus MC, University Medical Center Rotterdam, Rotterdam, the Netherlands.

When it comes to data storage, the field of flow cytometry is fairly standardized, thanks to the flow cytometry standard (FCS) file format. The structure of FCS files is described in the FCS specification. Software that strictly complies with the FCS specification, is guaranteed to be interoperable (in terms of exchange via FCS files). Read More

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http://dx.doi.org/10.1002/cyto.a.24187DOI Listing

Factors Predicting Participation in the Prospective Genomic Sequencing Study, Total Cancer Care (TCC), in Kentucky.

J Rural Health 2020 Jul 6. Epub 2020 Jul 6.

Department of Pharmacy, University of Kentucky, Lexington, Kentucky.

Purpose: Large-scale genomic sequencing studies are driving oncology drug development. However, rural populations, like those residing in Appalachian Kentucky, are underrepresented in these efforts. In this study, we determined the frequency of participation, reasons for nonparticipation, and factors predicting the decision to participate in the Total Cancer Care (TCC) prospective genomic cohort study. Read More

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http://dx.doi.org/10.1111/jrh.12492DOI Listing

Small noncoding RNA sRNA0426 is involved in regulating biofilm formation in Streptococcus mutans.

Microbiologyopen 2020 Jul 6:e1096. Epub 2020 Jul 6.

Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-Sen University, Guangzhou, China.

Evidence suggests that small noncoding RNAs (sRNAs) are involved in the complex regulatory networks governing biofilm formation. Few studies have investigated the role of sRNAs in Streptococcus mutans (S. mutans). Read More

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http://dx.doi.org/10.1002/mbo3.1096DOI Listing

pendymoma relapse goes along with a relatively stable epigenome, but a severely altered tumor morphology.

Brain Pathol 2020 Jul 6. Epub 2020 Jul 6.

Department of Pediatric Hematology and Oncology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.

The molecular biology of ependymomas is not well understood, and this is particularly true for ependymoma relapses. We aimed at finding out if, and to which extent, relapses differ from their corresponding primary tumors on the morphological, chromosomal, and epigenetic level. We investigated 24 matched ependymoma primary and relapsed tumor samples and, as a first step, compared cell density, necrosis, vessel proliferation, Ki67 proliferative index, trimethylation at H3K27, and expression of CXorf67. Read More

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http://dx.doi.org/10.1111/bpa.12875DOI Listing

Daudi cell stroma: An alternative to dithiothreitol to resolve daratumumab interference in pretransfusion testing.

Transfusion 2020 Jul 6. Epub 2020 Jul 6.

Medical Affairs and Innovation, Héma-Québec, Québec, Quebec, Canada.

Treatment of red blood cells with dithiothreitol (DTT) or trypsin effectively denatures CD38; however, this treatment damages other antigens, some of which are of clinical importance. Thus, other avenues to deplete daratumumab (DARA) from plasma samples should be explored.

Study Design And Methods: The Daudi B-cell line was found to express high levels of CD38 and was sonicated in a sonication buffer to achieve complete cell lysis. Read More

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http://dx.doi.org/10.1111/trf.15932DOI Listing

Integrated metabolomics reveals altered lipid metabolism in adipose tissue in a model of extreme longevity.

Geroscience 2020 Jul 6. Epub 2020 Jul 6.

Joslin Diabetes Center, Section on Integrative Physiology and Metabolism, Harvard Medical School, Boston, MA, USA.

Adipose tissue plays an essential role in metabolic health. Ames dwarf mice are exceptionally long-lived and display metabolically beneficial phenotypes in their adipose tissue, providing an ideal model for studying the intersection between adipose tissue and longevity. To this end, we assessed the metabolome and lipidome of adipose tissue in Ames dwarf mice. Read More

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http://dx.doi.org/10.1007/s11357-020-00221-0DOI Listing

Multiwalled carbon nanotubes modified with MoO nanoparticles for voltammetric determination of the pesticide oxyfluorfen.

Mikrochim Acta 2020 Jul 6;187(8):429. Epub 2020 Jul 6.

School of Materials Science and Engineering, University of New South Wales, Sydney, NSW, 2052, Australia.

A glassy carbon electrode was functionalized by MoO nanoparticle-decorated multiwalled carbon nanotubes (MWCNTs) and examined as a working electrode in oxyfluorfen (OXY) detection by differential pulse stripping voltammetry (DPSV). Measurement parameters were as follows: initial potential - 0.1 V, end potential + 0. Read More

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http://dx.doi.org/10.1007/s00604-020-04406-4DOI Listing

Correction to: A candidate gene study of intermediate histopathological phenotypes in HIV-associated neurocognitive disorders.

J Neurovirol 2020 Jul 6. Epub 2020 Jul 6.

Department of Psychiatry, University of California San Diego School of Medicine, San Diego, CA, USA.

Due to a production error data in Table 1 were not presented correctly. Read More

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http://dx.doi.org/10.1007/s13365-020-00871-yDOI Listing

De Novo Assembly and Annotation of the Juvenile Tuber Transcriptome of a Gastrodia elata Hybrid by RNA Sequencing: Detection of SSR Markers.

Biochem Genet 2020 Jul 6. Epub 2020 Jul 6.

Department of Field Crops, Faculty of Agricultural and Natural Sciences, Abant İzzet Baysal University, Bolu, Turkey.

Gastrodia elata is a traditional Chinese herbal medicine with good therapeutic effect on various nervous and cerebrovascular diseases. In the present study, we generated 20,611,556 raw reads from the young tuber transcriptome of a G. elata hybrid (Gastrodia elata BI. Read More

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http://dx.doi.org/10.1007/s10528-020-09983-wDOI Listing