410 results match your criteria Biofuels Bioproducts & Biorefining-biofpr[Journal]


A Novel Glucose-Tolerant GH1 β-Glucosidase and Improvement of Its Glucose Tolerance Using Site-Directed Mutation.

Appl Biochem Biotechnol 2020 Jul 3. Epub 2020 Jul 3.

Key Laboratory of Sustainable Development of Polar Fishery, Ministry of Agriculture and Rural Affairs, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, 266071, China.

A novel GH1 β-glucosidase gene (bgla) from marine bacterium was sequenced and expressed in Escherichia coli. After purification by Ni affinity chromatography, the recombinant protein was characterized. The purified recombinant enzyme showed maximum activity at 40 °C, pH 7. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s12010-020-03373-zDOI Listing

Biochemical characteristics and molecular mechanism of an exo-type alginate lyase VxAly7D and its use for the preparation of unsaturated monosaccharides.

Biotechnol Biofuels 2020 1;13:99. Epub 2020 Jun 1.

Key Laboratory of Marine Drugs, Ministry of Education; Shandong Provincial Key Laboratory of Glycoscience and Glycoengineering, School of Medicine and Pharmacy, Ocean University of China, 5 Yushan Road, Qingdao, 266003 China.

Background: As the most abundant polysaccharide in brown algae, alginate has become a promising economical material for bioethanol production. Recently, exo-type alginate lyases have received extensive attention because the unsaturated monosaccharides produced by their degradation of alginate can be easily converted into 4-deoxy-l--5-hexoseulose uronate (DEH), a promising material for bioethanol production and biorefinery systems.

Results: In this study, we cloned and characterized an exo-type polysaccharide lyase family 7 (PL7) alginate lyase VxAly7D from the marine bacterium QY104. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13068-020-01738-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7268478PMC

Bioaldehydes and beyond: Expanding the realm of bioderived chemicals using biogenic aldehydes as platforms.

Curr Opin Chem Biol 2020 May 23;59:37-46. Epub 2020 May 23.

Department of Biosystems Engineering, Auburn University, Auburn, AL 36849, USA; Center for Bioenergy and Bioproducts, Auburn University, Auburn, AL 36849, USA. Electronic address:

Biofuels and biochemicals derived from renewable resources are sconsidered as potential solutions for the energy crisis and associated environmental problems that human beings are facing today. However, so far the available types of bioderived chemicals are rather limited, and production efficiency is generally low. Expanding the realm of bioderived chemicals and relevant derivatives can help motivate the development of bioenergy and the general bioeconomy. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.cbpa.2020.04.007DOI Listing

RRNPP-type quorum-sensing systems regulate solvent formation, sporulation and cell motility in .

Biotechnol Biofuels 2020 8;13:84. Epub 2020 May 8.

1Department of Biosystems Engineering, Auburn University, 350 Mell Street, Auburn, AL 36849 USA.

Background: N1-4 (HMT) is a strictly anaerobic, spore-forming Gram-positive bacterium capable of hyper-butanol production through the well-known acetone-butanol-ethanol fermentation process. Recently, five putative RRNPP-type QSSs (here designated as QSS1 to QSS5) were predicted in this bacterial strain, each of which comprises a putative RRNPP-type regulator (QssR1 to QssR5) and a cognate signaling peptide precursor (QssP1 to QssP5). In addition, both proteins are encoded by the same operon. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13068-020-01723-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7206700PMC

Polyhydroxyalkanoates (PHA) production from biogas in waste treatment facilities: Assessing the potential impacts on economy, environment and society.

Chemosphere 2020 Sep 29;255:126929. Epub 2020 Apr 29.

Institute of Sustainable Processes, University of Valladolid, Dr. Mergelina, s/n, 47011, Valladolid, Spain; Department of Chemical and Environmental Engineering, University of Valladolid, Dr. Mergelina, s/n, 47011, Valladolid, Spain. Electronic address:

Using the biogas generated from organic waste anaerobic treatment to produce polyhydroxyalkanoates (PHAs) has emerged as an attractive alternative to heat and power generation (CHP) in waste treatment plants. The sustainability of biogas combustion for CHP, biogas bioconversion into PHA and a combination of both scenarios was compared in terms of environmental impact, process economics and social responsibility according to the IChemE Sustainability Metrics. Although PHA production presented higher investment and operational costs, a comparable economic performance was observed in all biogas valorization scenarios regarding net present value (0. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.chemosphere.2020.126929DOI Listing
September 2020

Enhancement of biogas production from wastewater sludge via anaerobic digestion assisted with biochar amendment.

Bioresour Technol 2020 Aug 11;309:123368. Epub 2020 Apr 11.

College of Marine Life Sciences, Ocean University of China, Qingdao 266100, China.

Studies have shown that biochar enhances methane formation due to the presence of redox active moieties and its conductive properties. This study investigated the influence of biochar, which was produced from Douglas fir pyrolysis, on biogas production and microbial community during anaerobic digestion (AD) of wastewater sludge. The results showed that biochar significantly enhances methane (CH) production rate and increases its final yield during AD. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.biortech.2020.123368DOI Listing

Investigation of alkaline hydrogen peroxide pretreatment to enhance enzymatic hydrolysis and phenolic compounds of oil palm trunk.

3 Biotech 2020 Apr 27;10(4):179. Epub 2020 Mar 27.

1Department of Biotechnology, Faculty of Agro-Industry, Kasetsart University, 50 Ngam Wong Wan Rd, Ladyaow, Chatuchak, Bangkok, 10900 Thailand.

Alkaline hydrogen peroxide (AHP) as a pretreatment effectively enhances the increasing enzymatic digestibility of oil palm trunk (OPT) for conversion to biofuels and bioproducts in the biorefinery processes. The effect of hydrogen peroxide concentration (1-5%), temperature (50-90 °C), and time (30-90 min) were studied to find out the optimum condition for the removal of lignin. The optimum condition attained at 70 °C, 30 min, and 3% HO g /g of biomass not only increased the cellulose content from 38. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s13205-020-02169-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7101454PMC

Accumulation of high-value bioproducts can improve the economics of advanced biofuels.

Proc Natl Acad Sci U S A 2020 Apr 27;117(15):8639-8648. Epub 2020 Mar 27.

Life-cycle, Economics, and Agronomy Division, Joint BioEnergy Institute, Lawrence Berkeley National Laboratory, Berkeley, CA 94720;

Coproduction of high-value bioproducts at biorefineries is a key factor in making biofuels more cost-competitive. One strategy for generating coproducts is to directly engineer bioenergy crops to accumulate bioproducts that can be fractionated and recovered at biorefineries. Here, we develop quantitative insights into the relationship between bioproduct market value and target accumulation rates by investigating a set of industrially relevant compounds already extracted from plant sources with a wide range of market prices and applications, including <$10/kg (limonene, latex, and polyhydroxybutyrate [PHB]), $10 to $100/kg (cannabidiol), and >$100/kg (artemisinin). Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1073/pnas.2000053117DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7165473PMC

Enzymatic production of 4--methyl d-glucaric acid from hardwood xylan.

Biotechnol Biofuels 2020 13;13:51. Epub 2020 Mar 13.

1Department of Chemical Engineering and Applied Chemistry, University of Toronto, Toronto, ON Canada.

Background: Dicarboxylic acids offer several applications in detergent builder and biopolymer fields. One of these acids, 4--methyl d-glucaric acid, could potentially be produced from glucuronoxylans, which are a comparatively underused fraction of wood and agricultural biorefineries.

Results: Accordingly, an enzymatic pathway was developed that combines AxyAgu115A, a GH115 α-glucuronidase from , and GOOX, an AA7 gluco-oligosaccharide oxidase from , to produce this bio-based chemical from glucuronoxylan. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13068-020-01691-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7071571PMC

Light regulation of light-harvesting antenna size substantially enhances photosynthetic efficiency and biomass yield in green algae.

Plant J 2020 Mar 17. Epub 2020 Mar 17.

New Mexico Consortium, Los Alamos, NM, 87544, USA.

One of the major factors limiting biomass productivity in algae is the low thermodynamic efficiency of photosynthesis. The greatest thermodynamic inefficiencies in photosynthesis occur during the conversion of light into chemical energy. At full sunlight the light-harvesting antenna captures photons at a rate nearly 10 times faster than the rate-limiting step in photosynthetic electron transport. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/tpj.14751DOI Listing
March 2020
5.972 Impact Factor

Integrated biorefinery approach to valorize winery waste: A review from waste to energy perspectives.

Sci Total Environ 2020 Jun 14;719:137315. Epub 2020 Feb 14.

State Key Laboratory of Crop Stress Biology in Arid Areas, College of Horticulture, Northwest A&F University, Yangling, Xianyang 712100, China; Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in Northwest China, Ministry of Agriculture, Northwest A&F University, Yangling, Xianyang 712100, China. Electronic address:

The ever-increasing environmental crisis, depleting natural resources, and uncertainties in fossil fuel availability have rekindled researchers' attention to develop green and environmentally friendlier strategies. In this context, a biorefinery approach with a zero-waste theme has stepped-up as the method of choice for sustainable production of an array of industrially important products to address bio-economy challenges. Grape winery results in substantial quantities of solid organic and effluent waste, which epitomizes an increasing concentration of pollution problems with direct damage to human health, economy and nature. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.scitotenv.2020.137315DOI Listing
June 2020
4.099 Impact Factor

Lignocellulosic biomass: Acid and alkaline pretreatments and their effects on biomass recalcitrance - Conventional processing and recent advances.

Bioresour Technol 2020 May 21;304:122848. Epub 2020 Jan 21.

Federal University of Paraná, Department of Bioprocess Engineering and Biotechnology, Centro Politécnico, 81531-990 Curitiba, Paraná, Brazil. Electronic address:

Lignocellulosic biomass is one of the most abundant organic resources worldwide and is a promising source of renewable energy and bioproducts. It basically consists of three fractions, cellulose, hemicelluloses and lignin, which confer a recalcitrant structure. As such, pretreatment steps are required to make each fraction available for further use, with acidic, alkaline and combined acidic-alkaline treatments being the most common techniques. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.biortech.2020.122848DOI Listing

Prospects of integrating algae technologies into landfill leachate treatment.

World J Microbiol Biotechnol 2020 Feb 24;36(3):39. Epub 2020 Feb 24.

University of South Florida, Patel College of Global Sustainability, 4202 East Fowler Avenue, CGS 101, Tampa, FL, 33620, USA.

Landfilling of municipal waste, an environmental challenge worldwide, results in the continuous formation of significant amounts of leachate, which poses a severe contamination threat to ground and surface water resources. Landfill leachate (LL) is generated by rainwater percolating through disposed waste materials and must be treated effectively before safe discharge into the environment. LL contains numerous pollutants and toxic substances, such as dissolved organic matter, inorganic chemicals, heavy metals, and anthropogenic organic compounds. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s11274-020-2810-yDOI Listing
February 2020

Potential utilization of bioproducts from microalgae for the quality enhancement of natural products.

Bioresour Technol 2020 May 11;304:122997. Epub 2020 Feb 11.

Department of Chemical and Environmental Engineering, Faculty of Science and Engineering, University of Nottingham Malaysia, Jalan Broga, Semenyih 43500, Selangor Darul Ehsan, Malaysia. Electronic address:

Microalgae are autotroph organisms that utilise light energy to synthesize various high-value bioactive compounds such as polysaccharides, proteins and lipids. Due to its fast growth rate and capability to survive in harsh environment, microalgae nowadays are applied in various industrial areas. The process of obtaining microalgae-based biomolecules starts with the selection of suitable microalgae strain, cultivation, followed by downstream processing of the biomass (i. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.biortech.2020.122997DOI Listing

Salicylic acid and aspirin stimulate growth of Chlamydomonas and inhibit lipoxygenase and chloroplast desaturase pathways.

Plant Physiol Biochem 2020 Apr 15;149:256-265. Epub 2020 Feb 15.

Chemical Engineering and Applied Chemistry, Energy and Bioproducts Research Institute, Aston University, B4 7ET, Birmingham, United Kingdom. Electronic address:

Chemical stimulants, used to enhance biomass yield, are highly desirable for the commercialisation of algal products for a wide range of applications in the food, pharma and biofuels sectors. In the present study, phenolic compounds, varying in substituents and positional isomers on the arene ring have been evaluated to determine structure-activity relationship and growth. The phenols, catechol, 4-methylcatechol and 2, 4-dimethyl phenol were generally inhibitory to growth as were the compounds containing an aldehyde function. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.plaphy.2020.02.019DOI Listing

A holistic zero waste biorefinery approach for macroalgal biomass utilization: A review.

Sci Total Environ 2020 May 1;716:137067. Epub 2020 Feb 1.

Department of Chemical Engineering, Chungbuk National University, Cheongju, Chunbuk 361-763, Republic of Korea. Electronic address:

The growing concerns over the depleting fossil fuels and increase in the release of greenhouse gas emissions have necessitated the search for the potential biomass source for alternative energy generation. In this context, third generation biomass specifically maroalgae has gained a lot of research interest in the recent years for energy and products generation such as ethanol, butanol, alginates, agars, and carrageenans. There are a few reviews available in scientific domain on macroalgal biomass utilization for bioethanol production but none of them has addressed precisely from phenolic precursor compounds to the entire ethanol production process and its bottlenecks. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.scitotenv.2020.137067DOI Listing
May 2020
4.099 Impact Factor

Recent advances on the sustainable approaches for conversion and reutilization of food wastes to valuable bioproducts.

Bioresour Technol 2020 Apr 24;302:122889. Epub 2020 Jan 24.

Biorefinery and Bioprocess Engineering Laboratory, Department of Chemical Engineering and Materials Science, Yuan Ze University, Chungli, Taoyuan 320, Taiwan. Electronic address:

The increasing amounts of food wastage and accumulation generated per annum due to the growing human population worldwide often associated with environmental pollution issues and scarcity of natural resources. In view of this, science community has worked towards in finding sustainable approaches to replace the common practices for food waste management. The agricultural and food processing wastes rich in nutrients are often the attractive substrates for the bioconversion for valuable bioproducts such as industrial enzymes, biofuel and bioactive compounds. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.biortech.2020.122889DOI Listing

Production of ent-kaurene from lignocellulosic hydrolysate in Rhodosporidium toruloides.

Microb Cell Fact 2020 Feb 5;19(1):24. Epub 2020 Feb 5.

Department of Energy, Agile BioFoundry, Emeryville, CA, 94608, USA.

Background: Rhodosporidium toruloides has emerged as a promising host for the production of bioproducts from lignocellulose, in part due to its ability to grow on lignocellulosic feedstocks, tolerate growth inhibitors, and co-utilize sugars and lignin-derived monomers. Ent-kaurene derivatives have a diverse range of potential applications from therapeutics to novel resin-based materials.

Results: The Design, Build, Test, and Learn (DBTL) approach was employed to engineer production of the non-native diterpene ent-kaurene in R. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1186/s12934-020-1293-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7003354PMC
February 2020
4.221 Impact Factor

Integrating pyrolysis and ex-situ catalytic reforming by microwave heating to produce hydrocarbon-rich bio-oil from soybean soapstock.

Bioresour Technol 2020 Apr 22;302:122843. Epub 2020 Jan 22.

Jiangxi University of Traditional Chinese Medicine, Nanchang, Jiangxi 330047, China.

The composite catalysts were synthesized with SiC powder and ZSM-5 and were characterized by Brunauer-Emmett-Teller, X-ray diffraction, thermogravimetric analysis, pyridine-infrared spectroscopy, and scanning electron microscopy. The catalysts showed a high heating rate and excellent catalytic performance for pyrolysis vapors, and the product fractional distribution and chemical compositions of bio-oil in a tandem system (microwave pyrolysis and microwave ex-situ catalytic reforming) was examined. Experimental results confirmed the quality of bio-oil produced by the microwave-induced catalytic reforming was better than that product through electric heating. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.biortech.2020.122843DOI Listing

Progress in the physicochemical treatment of microalgae biomass for value-added product recovery.

Bioresour Technol 2020 Apr 3;301:122727. Epub 2020 Jan 3.

Laboratory of Microbiology and Biochemistry, College of Chemistry and Food Engineering, Federal University of Rio Grande, Rio Grande-RS, Brazil.

Interest in microalgae-derived products is growing, mostly due to their unique characteristics and range of industrial applications. To obtain different products, one must employ specific pretreatments that retain the properties of the biologically active compounds extracted from microalgae biomass; thus, new extraction techniques require frequent upgrades. Due to increased interest in economically viable and ecologically friendly processes, new extraction methods that can be incorporated into microalgae biorefinery systems have become the main focus of research. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.biortech.2019.122727DOI Listing

Sustainability and life cycle assessments of lignocellulosic and algal pretreatments.

Bioresour Technol 2020 Apr 26;301:122678. Epub 2019 Dec 26.

Centre for Innovation and Translational Research, CSIR- Indian Institute of Toxicology Research (CSIR-IITR), 31 MG Marg, Lucknow 226 001, India.

Bioenergy and Bioproducts have gained augmented relevance in the wake of depleting fossil fuels and escalating environmental problems induced by anthropogenic activities. The paper outlays the various applications of biomass and their significance in various processes. The prospects of lignocelluloses and algal raw materials to biofuel production are well established; however the life cycle analysis of every bioprocess becomes essential for its technical feasibility. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.biortech.2019.122678DOI Listing

Exploiting nonionic surfactants to enhance fatty alcohol production in Rhodosporidium toruloides.

Biotechnol Bioeng 2020 May 11;117(5):1418-1425. Epub 2020 Feb 11.

Department of Biomass Science and Conversion Technology, Sandia National Laboratories, Livermore, California.

Fatty alcohols (FOHs) are important feedstocks in the chemical industry to produce detergents, cosmetics, and lubricants. Microbial production of FOHs has become an attractive alternative to production in plants and animals due to growing energy demands and environmental concerns. However, inhibition of cell growth caused by intracellular FOH accumulation is one major issue that limits FOH titers in microbial hosts. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/bit.27285DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7187362PMC

Nano-immobilized cellulases for biomass processing with application in biofuel production.

Methods Enzymol 2020 5;630:327-346. Epub 2019 Nov 5.

Centre for Marine Bioproducts Development, College of Medicine and Public Health, Flinders University, Bedford Park, SA, Australia. Electronic address:

Several researches have focused on the enzymatic pretreatment of lignocellulose biomass to produce fermentable sugars that can lead to ethanol production thus facilitating pathways for sustainable biofuel production. Enzymes are fundamental to the pretreatment process, however, are required in larger quantities during pretreatment process thus influencing biofuel production cost. Immobilization of enzymes to a suitable support/matrix could enhance its stability, and reusability thus containing cost. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/bs.mie.2019.09.006DOI Listing
November 2019

Systems and synthetic biology tools for advanced bioproduction hosts.

Curr Opin Biotechnol 2020 Jan 8;64:101-109. Epub 2020 Jan 8.

Joint BioEnergy Institute, Lawrence Berkeley National Laboratory, Emeryville, CA, USA; Agile BioFoundry, Lawrence Berkeley National Laboratory, Emeryville, CA, USA; Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA. Electronic address:

The genomic revolution ushered in an era of discovery and characterization of enzymes from novel organisms that fueled engineering of microbes to produce commodity and high-value compounds. Over the past decade advances in synthetic biology tools in recent years contributed to significant progress in metabolic engineering efforts to produce both biofuels and bioproducts resulting in several such related items being brought to market. These successes represent a burgeoning bio-economy; however, significant resources and time are still necessary to progress a system from proof-of-concept to market. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.copbio.2019.12.007DOI Listing
January 2020

Recent developments in pretreatment technologies on lignocellulosic biomass: Effect of key parameters, technological improvements, and challenges.

Bioresour Technol 2020 Mar 2;300:122724. Epub 2020 Jan 2.

Department of Biological Engineering, College of Engineering, Konkuk University, Seoul 05029, Republic of Korea; Institute for Ubiquitous Information Technology and Application, Konkuk University, Seoul 05029, Republic of Korea. Electronic address:

Lignocellulosic biomass is an inexpensive renewable source that can be used to produce biofuels and bioproducts. The recalcitrance nature of biomass hampers polysaccharide accessibility for enzymes and microbes. Several pretreatment methods have been developed for the conversion of lignocellulosic biomass into value-added products. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.biortech.2019.122724DOI Listing

Lignocellulosic Biomass: Understanding Recalcitrance and Predicting Hydrolysis.

Front Chem 2019 18;7:874. Epub 2019 Dec 18.

FARE Laboratory, INRAE, University of Reims Champagne-Ardenne, Reims, France.

Lignocellulosic biomass (LB) is an abundant and renewable resource from plants mainly composed of polysaccharides (cellulose and hemicelluloses) and an aromatic polymer (lignin). LB has a high potential as an alternative to fossil resources to produce second-generation biofuels and biosourced chemicals and materials without compromising global food security. One of the major limitations to LB valorisation is its recalcitrance to enzymatic hydrolysis caused by the heterogeneous multi-scale structure of plant cell walls. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3389/fchem.2019.00874DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6930145PMC
December 2019

Engineering of Saccharomyces cerevisiae for efficient fermentation of cellulose.

FEMS Yeast Res 2020 Feb;20(1)

Department of Food Science and Human Nutrition, 905 S. Goodwin Ave., IL 61801, USA.

Conversion of lignocellulosic biomass to biofuels using microbial fermentation is an attractive option to substitute petroleum-based production economically and sustainably. The substantial efforts to design yeast strains for biomass hydrolysis have led to industrially applicable biological routes. Saccharomyces cerevisiae is a robust microbial platform widely used in biofuel production, based on its amenability to systems and synthetic biology tools. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1093/femsyr/foz089DOI Listing
February 2020

Production of tetra-methylpyrazine using engineered .

Metab Eng Commun 2020 Jun 2;10:e00115. Epub 2019 Dec 2.

Joint BioEnergy Institute, Emeryville, CA, USA.

ATCC 13032 is an established and industrially-relevant microbial host that has been utilized for the expression of many desirable bioproducts. Tetra-methylpyrazine (TMP) is a naturally occurring alkylpyrazine with broad applications spanning fragrances to resins. We identified an engineered strain of which produces 5 ​g/L TMP and separately, a strain which can co-produce both TMP and the biofuel compound isopentenol. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.mec.2019.e00115DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6926172PMC

Correction to: Production efficiency of the bacterial non-ribosomal peptide indigoidine relies on the respiratory metabolic state in S. cerevisiae.

Microb Cell Fact 2019 Dec 29;18(1):218. Epub 2019 Dec 29.

Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA.

Following publication of the original article [1], the authors have noted that the standard curve in Additional file 1: Figure S7 is incorrect. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1186/s12934-019-1262-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6936116PMC
December 2019

Microwave-assisted catalytic upgrading of co-pyrolysis vapor using HZSM-5 and MCM-41 for bio-oil production: Co-feeding of soapstock and straw in a downdraft reactor.

Bioresour Technol 2020 Mar 14;299:122611. Epub 2019 Dec 14.

State Key Laboratory of Food Science and Technology, Engineering Research Center for Biomass Conversion, Ministry of Education, Nanchang University, Nanchang 330047, China; Center for Biorefining and Department of Bioproducts and Biosystems Engineering University of Minnesota, 1390 Eckles Ave., St. Paul, MN 55108, USA.

Microwave-assisted co-pyrolysis of low hydrogen-to-carbon and high hydrogen-to-carbon effective ratio materials with the aid of HZSM-5 and MCM-41 is a promising technique to improve the bio-oil quality. The low content of hydrocarbons and short life cycle of catalyst limit the application of pyrolysis technology in biomass energy conversion. The effects of catalytic temperature, and HZSM-5-to-MCM-41, feedstock-to-catalyst, and straw-to-soapstock ratios on the yield and composition of bio-oil were studied in this work. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.biortech.2019.122611DOI Listing
March 2020
4.494 Impact Factor

Genomic resources for energy cane breeding in the post genomics era.

Comput Struct Biotechnol J 2019 11;17:1404-1414. Epub 2019 Nov 11.

Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, Av. Prof. Lineu Prestes, 748, São Paulo 05508-000, SP, Brazil.

Sugarcane is one of the most sustainable energy crops among cultivated crops presenting the highest tonnage of cultivated plants. Its high productivity of sugar, bioethanol and bioelectricity make it a promising green alternative to petroleum. Furthermore, the myriad of products that can be derived from sugarcane biomass has been driving breeding programs towards varieties with a higher yield of fiber and a more vigorous and sustainable performance: the energy cane. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.csbj.2019.10.006DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6906722PMC
November 2019

Elucidating the influence of environmental factors on biogas-based polyhydroxybutyrate production by Methylocystis hirsuta CSC1.

Sci Total Environ 2020 Mar 21;706:135136. Epub 2019 Nov 21.

Department of Chemical Engineering and Environmental Technology, School of Industrial Engineering, University of Valladolid, Dr. Mergelina s/n, 47011 Valladolid, Spain; Institute of Sustainable Processes, Dr. Mergelina s/n, 47011 Valladolid, Spain. Electronic address:

The valorization of biogas as a feedstock for the generation of added-value bioproducts will play a key role on the sustainability of anaerobic digestion. The present work assessed the influence of key environmental parameters (O:CH ratio, temperature and nitrogen source) on the growth and polyhydroxybutyrate (PHB) synthesis under nitrogen limiting conditions of the type II methanotroph Methylocystis hirsuta CSC1 using biogas as a feedstock. The O:CH ratios tested (1:1, 1. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.scitotenv.2019.135136DOI Listing

Critical processes and variables in microalgae biomass production coupled with bioremediation of nutrients and CO from livestock farms: A review.

Sci Total Environ 2020 May 21;716:135247. Epub 2019 Nov 21.

Tecnologico de Monterrey, School of Engineering and Sciences, Campus Monterrey, Ave. Eugenio Garza Sada 2501, CP 64849, Monterrey, NL., Mexico.

Development of renewable and clean energy as well as bio-based fine chemicals technologies are the keys to overcome the problems such as fossil depletion, global warming, and environment pollution. To date, cultivation of microalgae using wastewater is regarded as a promising approach for simultaneous nutrients bioremediation and biofuels production due to their high photosynthesis efficiency and environmental benefits. However, the efficiency of nutrients removal and biomass production strongly depends on wastewater properties and microalgae species. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.scitotenv.2019.135247DOI Listing
May 2020
4.099 Impact Factor

Fast microwave-assisted pyrolysis of wastes for biofuels production - A review.

Bioresour Technol 2020 Feb 25;297:122480. Epub 2019 Nov 25.

Center for Biorefining, and Department of Bioproducts and Biosystems Engineering, 1390 Eckles Ave, St. Paul, MN 55108, USA; Ministry of Education Engineering Research Center for Biomass Conversion, Nanchang University, 235 Nanjing Road, Nanchang City, Jiangxi 330047, China. Electronic address:

Microwave-assisted pyrolysis of waste suffers from the problem that the waste generally has low microwave absorptivity thereby resulting in low heating rate and low pyrolysis temperature. In this case, fast microwave-assisted pyrolysis is proposed and developed to help the pyrolysis of waste. This study describes two methods that can be used to realize fast microwave-assisted pyrolysis of waste: (1) premixed method (wastes are mixed with microwave absorbent) and (2) non-premixed method (wastes are poured onto the heated microwave absorbent bed). Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.biortech.2019.122480DOI Listing
February 2020

Genomic analysis of sp. ISTR5 for biofunneling of lignin-derived compounds.

Biotechnol Biofuels 2019 27;12:277. Epub 2019 Nov 27.

School of Environmental Sciences, Jawaharlal Nehru University, New Delhi, 110067 India.

Background: Lignin is the second most abundant natural polymer on earth. Industries using lignocellulosic biomass as feedstock generate a considerable amount of lignin as a byproduct with minimal usage. For a sustainable biorefinery, the lignin must be utilized in improved ways. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13068-019-1606-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6880542PMC
November 2019

Multiomic Fermentation Using Chemically Defined Synthetic Hydrolyzates Revealed Multiple Effects of Lignocellulose-Derived Inhibitors on Cell Physiology and Xylose Utilization in .

Front Microbiol 2019 7;10:2596. Epub 2019 Nov 7.

DOE-Great Lakes Bioenergy Research Center, University of Wisconsin-Madison, Madison, WI, United States.

Utilization of both C5 and C6 sugars to produce biofuels and bioproducts is a key goal for the development of integrated lignocellulosic biorefineries. Previously we found that although engineered 2032 was able to ferment glucose to ethanol when fermenting highly concentrated hydrolyzates such as 9% glucan-loading AFEX-pretreated corn stover hydrolyzate (9% ACSH), xylose conversion after glucose depletion was greatly impaired. We hypothesized that impaired xylose conversion was caused by lignocellulose-derived inhibitors (LDIs) in hydrolyzates. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3389/fmicb.2019.02596DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6853872PMC
November 2019

Feasibility of commercial waste biorefineries for cassava starch industries: Techno-economic assessment.

Bioresour Technol 2020 Feb 22;297:122461. Epub 2019 Nov 22.

Department of Process Engineering, University of Stellenbosch, Private Bag X1, Stellenbosch 7602, South Africa. Electronic address:

Cassava waste is a potential bioresource for integrated biorefineries to co-produce bioproducts [succinic acid (SA), glucose syrup (GS), bioethanol] and combined heat and power (CHP). Techno-economic assessments of five biorefinery scenarios for integration in cassava starch plant (200 Mg/d), co-processing 377.83 Mg/h wastewater (CWW), 7. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.biortech.2019.122461DOI Listing
February 2020

An Effective Strategy for Identification of Highly Unstable Bacillaenes.

J Nat Prod 2019 12 27;82(12):3340-3346. Epub 2019 Nov 27.

Key Laboratory of Marine Drugs, Ministry of Education, School of Medicine and Pharmacy , Ocean University of China , Qingdao 266003 , China.

Exploration of unstable compounds is a rarely explored area of natural product research. We describe the integration of genomic and metabolomic analyses with bioassay-guided compound mining to effectively explore unstable bacillaenes. New bacillaene structures (, , and ) were identified from compound mixtures using the DANS-SVI (ifferential nalysis of 2D MR pectrum-ingle spectrum with ariable ntensities) method, which were further verified by the isolation of the pure compounds under strictly controlled conditions. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.jnatprod.9b00609DOI Listing
December 2019

Biomolecules from municipal and food industry wastes: An overview.

Bioresour Technol 2020 Feb 28;298:122346. Epub 2019 Oct 28.

Department of Chemical Engineering, Konkuk University, 1 Hwayang-Dong, Gwangjin-Gu, Seoul 05029, Republic of Korea. Electronic address:

Biological wastes generated from food and fruit processing industries, municipal markets, and water treatment facilities are a major cause of concern for Health Departments and Environmentalists around the world. Conventional means of managing these wastes such as transportation, treatment, and disposal, are proving uneconomical. The need is to develop green and sustainable technologies to circumvent this ever-growing and persistent problem. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.biortech.2019.122346DOI Listing
February 2020

Metabolic engineering for efficient production of icariside D2.

Biotechnol Biofuels 2019 6;12:261. Epub 2019 Nov 6.

1Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University, Yaguan Road 135, Jinnan District, Tianjin, 300350 China.

Background: Icariside D2 is a plant-derived natural glycoside with pharmacological activities of inhibiting angiotensin-converting enzyme and killing leukemia cancer cells. Production of icariside D2 by plant extraction and chemical synthesis is inefficient and environmentally unfriendly. Microbial cell factory offers an attractive route for economical production of icariside D2 from renewable and sustainable bioresources. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13068-019-1601-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6833136PMC
November 2019

Protein hydrolysis and fermentation under methanogenic and acidifying conditions.

Biotechnol Biofuels 2019 26;12:254. Epub 2019 Oct 26.

1Department of Environmental Technology, Wageningen University and Research, Bornse Weilanden 9, 6708 WG Wageningen, The Netherlands.

Background: Many kinds of wastewaters contain appreciable quantities of protein. Anaerobic processes are suitable for the treatment of wastewater high in organics to achieve pollution control and recovery of energy as methane and hydrogen, or intermediates for production of biofuels and valuable biochemicals. A distinction between protein hydrolysis and amino acid fermentation, especially for dissolved proteins, is needed to target which one is truly rate-limiting and to effectively harvest bioproducts during anaerobic conversion of these wastewaters. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13068-019-1592-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6815036PMC
October 2019

Production of fatty acid methyl esters and bioactive compounds from citrus wax.

Waste Manag 2020 Feb 24;102:48-55. Epub 2019 Oct 24.

Research Group of NanoBioscience, School of Chemistry, Autonomous University of Coahuila, 25280 Saltillo, Coahuila, Mexico. Electronic address:

Citrus wax is a waste generated during the purification process of the citrus essential oil. A lot of citrus wax wastes are globally produced, despite this, its composition and properties are not well known. Here we present comprehensive results proving the chemical composition and the physical properties of citrus wax. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.wasman.2019.10.021DOI Listing
February 2020

Biochar facilitated bioprocessing and biorefinery for productions of biofuel and chemicals: A review.

Bioresour Technol 2020 Jan 9;295:122252. Epub 2019 Oct 9.

School of Bioengineering, Dalian University of Technology, Dalian 116024, Liaoning, China.

Biochar is traditionally used to improve soil properties in arable land and as adsorbent or precursor of activated carbon in wastewater treatment. Recent advances have shown biochar potentials in enhancing productions of biofuels and chemicals such as bio-ethanol, butanol, methane, hydrogen, bio-diesel, hydrocarbons and carboxylic acids. The properties of biochar such as high levels of porosity, functional groups, cation exchange capacity, pH buffering capacity, electron conductivity, and macro-/micro- nutrients (Na, K, Ca, Mg, P, S, Fe, etc. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.biortech.2019.122252DOI Listing
January 2020

Production of galactitol from galactose by the oleaginous yeast IFO0880.

Biotechnol Biofuels 2019 18;12:250. Epub 2019 Oct 18.

1Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, 600 S. Mathews Ave., Urbana, IL 61801 USA.

Background: Sugar alcohols are commonly used as low-calorie sweeteners and can serve as potential building blocks for bio-based chemicals. Previous work has shown that the oleaginous yeast IFO0880 can natively produce arabitol from xylose at relatively high titers, suggesting that it may be a useful host for sugar alcohol production. In this work, we explored whether can produce additional sugar alcohols. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13068-019-1586-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6798376PMC
October 2019
1 Read

Relationship between sugarcane culm and leaf biomass composition and saccharification efficiency.

Biotechnol Biofuels 2019 17;12:247. Epub 2019 Oct 17.

1Queensland Alliance for Agriculture and Food Innovation, University of Queensland, Brisbane, QLD 4072 Australia.

Background: Lignocellulosic biomass is recognized as a promising renewable feedstock for the production of biofuels. However, current methods for converting biomass into fermentable sugars are considered too expensive and inefficient due to the recalcitrance of the secondary cell wall. Biomass composition can be modified to create varieties that are efficiently broken down to release cell wall sugars. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13068-019-1588-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6796448PMC
October 2019

A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products.

J Vis Exp 2019 10 4(152). Epub 2019 Oct 4.

Department of Plant Biology, University of California Davis;

Diterpenoids form a diverse class of small molecule natural products that are widely distributed across the kingdoms of life and have critical biological functions in developmental processes, interorganismal interactions, and environmental adaptation. Due to these various bioactivities, many diterpenoids are also of economic importance as pharmaceuticals, food additives, biofuels, and other bioproducts. Advanced genomics and biochemical approaches have enabled a rapid increase in the knowledge of diterpenoid-metabolic genes, enzymes, and pathways. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3791/59992DOI Listing
October 2019

Specific quantification of Scenedesmus obliquus and Chlorella vulgaris in mixed-species algal biofilms.

Bioresour Technol 2020 Jan 9;295:122251. Epub 2019 Oct 9.

Department of Biology, University of Toronto, Mississauga, 3359 Mississauga Road North, Mississauga L5L 1C6, Ontario, Canada. Electronic address:

Two TaqMan® qPCR assays were developed to specifically quantify the absolute abundance of Scenedesmus obliquus and Chlorella vulgaris in mixed-species algal biofilms by targeting the psbA gene. Standard curves were developed with amplification efficiencies of 92.4% and 96. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.biortech.2019.122251DOI Listing
January 2020

A review on commercial-scale high-value products that can be produced alongside cellulosic ethanol.

Biotechnol Biofuels 2019 8;12:240. Epub 2019 Oct 8.

Department of Biotechnology, Lorena School of Engineering, University of Sao Paulo, Estrada Municipal do Campinho, Lorena, SP CEP 12602-810 Brazil.

The demand for fossil derivate fuels and chemicals has increased, augmenting concerns on climate change, global economic stability, and sustainability on fossil resources. Therefore, the production of fuels and chemicals from alternative and renewable resources has attracted considerable and growing attention. Ethanol is a promising biofuel that can reduce the consumption of gasoline in the transportation sector and related greenhouse gas (GHG) emissions. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13068-019-1529-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6781352PMC
October 2019

Efficient microalgae harvesting using a thermal flotation method with response surface methodology.

Water Sci Technol 2019 Aug;80(3):426-436

School of Environmental Science and Engineering, Chang'an University, Xi'an 710054, China E-mail: Key Laboratory of Subsurface Hydrology and Ecology in Arid Areas, Ministry of Education, Xi'an 710054, China and Shaanxi Key Laboratory of Land Consolidation, Xi'an 710075, China.

Thermal pre-flocculation to enable dispersed air flotation is an economical and ecofriendly technology for harvesting microalgae from water. However, the underlying mechanism and optimal conditions for this method remain unclear. In this study, Chlorella vulgaris (C. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.2166/wst.2019.287DOI Listing

Bioprocess strategies for enhancing biomolecules productivity in Chlorella fusca LEB 111 using CO a carbon source.

Biotechnol Prog 2020 Mar 4;36(2):e2909. Epub 2019 Nov 4.

Laboratory of Biochemical Engineering, College of Chemistry and Food Engineering, Federal University of Rio Grande, Rio Grande, RS, Brazil.

The aim of this study was to evaluate the influence of different carbon dioxide (CO ) concentrations on the distribution of carbon forms in the culture medium and the biomass production and biomolecules productivity of the strain Chlorella fusca LEB 111. In this study, experiments were carried out in which C. fusca cultures were exposed to different CO concentrations, 0. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/btpr.2909DOI Listing