336 results match your criteria Biofuels Bioproducts & Biorefining-biofpr[Journal]


Adopting a Theophylline-Responsive Riboswitch for Flexible Regulation and Understanding of Glycogen Metabolism in PCC7942.

Front Microbiol 2019 21;10:551. Epub 2019 Mar 21.

Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, China.

Cyanobacteria are supposed to be promising photosynthetic microbial platforms that recycle carbon dioxide driven into biomass and bioproducts by solar energy. Glycogen synthesis serves as an essential natural carbon sink mechanism, storing a large portion of energy and organic carbon source of photosynthesis. Engineering glycogen metabolism to harness and rewire carbon flow is an important strategy to optimize efficacy of cyanobacteria platforms. Read More

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http://dx.doi.org/10.3389/fmicb.2019.00551DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6437101PMC
March 2019
1 Read

Palm oil and its wastes as bioenergy sources: a comprehensive review.

Environ Sci Pollut Res Int 2019 Apr 2. Epub 2019 Apr 2.

Institute of Sustainable Energy, Universiti Tenaga Nasional, 43000, Kajang, Selangor, Malaysia.

Due to global warming and increasing price of fossil fuel, scientists all over the world have been trying to find reliable alternative fuels. One of the most potential candidates is renewable energy from biomass. The race for renewable energy from biomass has long begun and focused on to combat the deteriorating condition of the environment. Read More

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http://link.springer.com/10.1007/s11356-019-04563-x
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http://dx.doi.org/10.1007/s11356-019-04563-xDOI Listing
April 2019
6 Reads

Development of a closed-loop process for fusel alcohol production and nutrient recycling from microalgae biomass.

Bioresour Technol 2019 Jul 2;283:350-357. Epub 2019 Mar 2.

Department of Biomass Science & Conversion Technologies, Sandia National Laboratories, Livermore, CA 94550, USA. Electronic address:

Improving the economic feasibility is necessary for algae-based processes to achieve commercial scales for biofuels and bioproducts production. A closed-loop system for fusel alcohol production from microalgae biomass with integrated nutrient recycling was developed, which enables the reuse of nitrogen and phosphorus for downstream application and thus reduces the operational requirement for external major nutrients. Mixed fusel alcohols, primarily isobutanol and isopentanol were produced from Microchloropsis salina hydrolysates by an engineered E. Read More

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http://dx.doi.org/10.1016/j.biortech.2019.03.006DOI Listing
July 2019
3 Reads
4.494 Impact Factor

Biofuel and bioproduct environmental sustainability analysis.

Authors:
Jennifer B Dunn

Curr Opin Biotechnol 2019 Mar 28;57:88-93. Epub 2019 Mar 28.

Chemical and Biological Engineering, Northwestern University, United States; Northwestern-Argonne Institute of Science and Engineering, 1-160 Hogan Building, Evanston, IL, United States. Electronic address:

Life cycle analysis (LCA) is a key tool in the evaluation of biofuel and bioproduct sustainability. Recent advances in these analyses include increased incorporation of spatially explicit elements of feedstock growth including changes in soil carbon and fertilization rates. Furthermore, new evaluations of processes to convert biomass to fuels (ethanol, algal-derived fuels, jet fuels, and others) and products have been conducted that examine emerging conversion technologies. Read More

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http://dx.doi.org/10.1016/j.copbio.2019.02.008DOI Listing
March 2019
1 Read

Multiplexed CRISPR-Cas9-Based Genome Editing of .

mSphere 2019 03 20;4(2). Epub 2019 Mar 20.

Energy Biosciences Institute, Berkeley, California, USA

Microbial production of biofuels and bioproducts offers a sustainable and economic alternative to petroleum-based fuels and chemicals. The basidiomycete yeast is a promising platform organism for generating bioproducts due to its ability to consume a broad spectrum of carbon sources (including those derived from lignocellulosic biomass) and to naturally accumulate high levels of lipids and carotenoids, two biosynthetic pathways that can be leveraged to produce a wide range of bioproducts. While has great potential, it has a more limited set of tools for genetic engineering relative to more advanced yeast platform organisms such as and Significant advancements in the past few years have bolstered ' engineering capacity. Read More

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http://dx.doi.org/10.1128/mSphere.00099-19DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6429044PMC

Monoterpene production by the carotenogenic yeast Rhodosporidium toruloides.

Microb Cell Fact 2019 Mar 18;18(1):54. Epub 2019 Mar 18.

Biomass Science and Conversion Technology, Sandia National Laboratories, 7011 East Ave, Livermore, CA, 94551, USA.

Background: Due to their high energy density and compatible physical properties, several monoterpenes have been investigated as potential renewable transportation fuels, either as blendstocks with petroleum or as drop-in replacements for use in vehicles (both heavy and light-weight) or in aviation. Sustainable microbial production of these biofuels requires the ability to utilize cheap and readily available feedstocks such as lignocellulosic biomass, which can be depolymerized into fermentable carbon sources such as glucose and xylose. However, common microbial production platforms such as the yeast Saccharomyces cerevisiae are not naturally capable of utilizing xylose, hence requiring extensive strain engineering and optimization to efficiently utilize lignocellulosic feedstocks. Read More

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http://dx.doi.org/10.1186/s12934-019-1099-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6421710PMC
March 2019
4.221 Impact Factor

Study of landfill leachate as a sustainable source of water and nutrients for algal biofuels and bioproducts using the microalga Picochlorum oculatum in a novel scalable bioreactor.

Bioresour Technol 2019 Jun 2;282:18-27. Epub 2019 Mar 2.

Patel College of Global Sustainability, University of South Florida, 4202 East Fowler Avenue, CGS 101, Tampa, FL 33620, USA. Electronic address:

High water demand is a major challenge for the algae industry, so cultivating algae in wastewater can have the double benefit of biomass production and water remediation. The use of landfill leachate (LL), which is wastewater generated in landfills, was investigated to grow the microalga Picochlorum oculatum in a novel horizontal bioreactor (HBR), a low-cost modular cultivation system that reduces water evaporation and contamination risk thanks to its enclosed design. Pilot-scale (150 L) and commercial-scale (2000 L) HBRs that were operated outdoors in Florida using LL in batch and semi-continuous modes generated high cell density cultures (1. Read More

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http://dx.doi.org/10.1016/j.biortech.2019.03.003DOI Listing
June 2019
1 Read

Engineering Robust Production Microbes for Large-Scale Cultivation.

Trends Microbiol 2019 Feb 25. Epub 2019 Feb 25.

Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA; Joint BioEnergy Institute, Lawrence Berkeley National Laboratory, Emeryville, CA 94608, USA; Environmental Genomics and Systems Biology Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA. Electronic address:

Systems biology and synthetic biology are increasingly used to examine and modulate complex biological systems. As such, many issues arising during scaling-up microbial production processes can be addressed using these approaches. We review differences between laboratory-scale cultures and larger-scale processes to provide a perspective on those strain characteristics that are especially important during scaling. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S0966842X193001
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http://dx.doi.org/10.1016/j.tim.2019.01.006DOI Listing
February 2019
9 Reads

Systems-Level Analysis of Oxygen Exposure in : Implications for Isoprenoid Production.

mSystems 2019 Jan-Feb;4(1). Epub 2019 Feb 12.

DOE Great Lakes Bioenergy Research Center, University of Wisconsin-Madison, Madison, Wisconsin, USA.

Zymomonas mobilis is an aerotolerant anaerobe and prolific ethanologen with attractive characteristics for industrial bioproduct generation. However, there is currently insufficient knowledge of the impact that environmental factors have on flux through industrially relevant biosynthetic pathways. Here, we examined the effect of oxygen exposure on metabolism and gene expression in Z. Read More

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http://dx.doi.org/10.1128/mSystems.00284-18DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6372839PMC
February 2019
2 Reads

Heterologous co-expression of two β-glucanases and a cellobiose phosphorylase resulted in a significant increase in the cellulolytic activity of the Caldicellulosiruptor bescii exoproteome.

J Ind Microbiol Biotechnol 2019 Feb 20. Epub 2019 Feb 20.

Department of Genetics, University of Georgia, Athens, GA, 30602, USA.

The ability to deconstruct plant biomass without conventional pretreatment has made members of the genus Caldicellulosiruptor the target of investigation for the consolidated processing of plant lignocellulosic biomass to biofuels and bioproducts. To investigate the synergy of enzymes involved and to further improve the ability of C. bescii to degrade cellulose, we introduced CAZymes that act synergistically with the C. Read More

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http://dx.doi.org/10.1007/s10295-019-02150-0DOI Listing
February 2019

Engineered microbial host selection for value-added bioproducts from lignocellulose.

Biotechnol Adv 2019 Feb 13. Epub 2019 Feb 13.

Department of Biochemistry and Immunology, Ribeirao Preto Medical School, University of São Paulo, Ribeirão Preto, SP, Brazil. Electronic address:

Lignocellulose is a rich and sustainable globally available carbon source and is considered a prominent alternative raw material for producing biofuels and valuable chemical compounds. Enzymatic hydrolysis is one of the crucial steps of lignocellulose degradation. Cellulolytic and hemicellulolytic enzyme mixes produced by different microorganisms including filamentous fungi, yeasts and bacteria, are used to degrade the biomass to liberate monosaccharides and other compounds for fermentation or conversion to value-added products. Read More

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http://dx.doi.org/10.1016/j.biotechadv.2019.02.003DOI Listing
February 2019

Optimization of microbial cell disruption using pressurized CO for improving lipid recovery from wet biomass.

Bioprocess Biosyst Eng 2019 Feb 1. Epub 2019 Feb 1.

Dave C. Swalm School of Chemical Engineering, Mississippi State University, Mississippi State, MS, 39762, USA.

Microbial cell disruption using pressurized gases (e.g., CO) is a promising approach to improve the lipid recovery from wet oleaginous microorganisms by eliminating the energy-intensive drying required for conventional methods. Read More

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http://dx.doi.org/10.1007/s00449-019-02080-2DOI Listing
February 2019

Investigation of the disruption of algal biomass with chlorine.

BMC Plant Biol 2019 Jan 9;19(1):18. Epub 2019 Jan 9.

Department of Civil, Construction and Environmental Engineering, San Diego State University, 5500 Campanile Drive, San Diego, CA, 92182, USA.

Background: Algal biofuel has a potential for reducing dependence on fossil fuel while curbing CO emissions. Despite these potential benefits, a scalable, sustainable, and commercially viable system has not yet been developed. One of the key barriers is the lack of viable methods for disrupting algal biomass for the separation and extraction of bioproducts. Read More

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http://dx.doi.org/10.1186/s12870-018-1614-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6327449PMC
January 2019
1 Read

Ten years of algal biofuel and bioproducts: gains and pains.

Planta 2019 Jan 2;249(1):195-219. Epub 2019 Jan 2.

Key Laboratory of Algal Biology, Institute of Hydrobiology, Chinese Academy of Sciences, 7 South Donghu Rd., Wuhan, 430072, Hubei Province, China.

Main Conclusion: It has been proposed that future efforts should focus on basic studies, biotechnology studies and synthetic biology studies related to algal biofuels and various high-value bioproducts for the economically viable production of algal biof uels. In recognition of diminishing fossil fuel reserves and the worsening environment, microalgal biofuel has been proposed as a renewable energy source with great potential. Algal biofuel thus became one of the hottest topics in renewable energy research in the new century, especially over the past decade. Read More

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http://dx.doi.org/10.1007/s00425-018-3066-8DOI Listing
January 2019
1 Read

Yeast screening and cell immobilization on inert supports for ethanol production from cheese whey permeate with high lactose loads.

PLoS One 2018 31;13(12):e0210002. Epub 2018 Dec 31.

Chemical and Environmental Bioprocess Engineering Group, Natural Resources Institute (IRENA), University of León, León, Spain.

Eight yeast strains of the genera Saccharomyces and Kluyveromyces were screened to ferment high lactose-load cheese whey permeate (CWP) (>130 g/L lactose) without nutrient supplementation. The fermentation conditions (temperature, pH and time) were optimized to maximize the fermentation performance (ethanol titer, ethanol yield and lactose consumption) for the two preselected strains, K. marxianus DSM 5422 and S. Read More

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0210002PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6312371PMC
December 2018
8 Reads
3.234 Impact Factor

Co-generation of ethanol and l-lactic acid from corn stalk under a hybrid process.

Biotechnol Biofuels 2018 18;11:331. Epub 2018 Dec 18.

1Fermentation Engineering Technology Research Center of Heibei Province, College of Bioscience & Bioengineering, Hebei University of Science and Technology, Shijiazhuang, 050000 People's Republic of China.

Background: Corn stover, as one important lignocellulosic material, has characteristics of low price, abundant output and easy availability. Using corn stover as carbon source in the fermentation of valuable organic chemicals contributes to reducing the negative environmental problems and the cost of production. In ethanol fermentation based on the hydrolysate of corn stover, the conversion rate of fermentable sugars is at a low level because the native does not utilize xylose. Read More

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http://dx.doi.org/10.1186/s13068-018-1330-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6298020PMC
December 2018
1 Read

Production efficiency of the bacterial non-ribosomal peptide indigoidine relies on the respiratory metabolic state in S. cerevisiae.

Microb Cell Fact 2018 Dec 13;17(1):193. Epub 2018 Dec 13.

Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA.

Background: Beyond pathway engineering, the metabolic state of the production host is critical in maintaining the efficiency of cellular production. The biotechnologically important yeast Saccharomyces cerevisiae adjusts its energy metabolism based on the availability of oxygen and carbon sources. This transition between respiratory and non-respiratory metabolic state is accompanied by substantial modifications of central carbon metabolism, which impact the efficiency of metabolic pathways and the corresponding final product titers. Read More

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https://microbialcellfactories.biomedcentral.com/articles/10
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http://dx.doi.org/10.1186/s12934-018-1045-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6293659PMC
December 2018
8 Reads

Fibre degradation of wheat straw by Pleurotus erygnii under low moisture conditions during solid-state fermentation.

Lett Appl Microbiol 2019 Feb 2;68(2):182-187. Epub 2019 Jan 2.

School of Environment, Natural Resources and Geography, Bangor University, Bangor, Gwynedd, UK.

The application of solid-state fermentation offers an alternative to conventional, submerged approaches for a variety of bioconversion processes, including animal feeds, biofuels and fungal bioproducts. Optimizing solid-state fermentation under low moisture conditions could significantly impact the proportion of dry biomass that could be processed and improve the commercial viability of this approach, because of reduced input costs and higher yields of final products. Pleurotus erygnii that appeared to show tolerance to low moisture conditions was grown on saturated and desaturated wheat straw. Read More

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http://doi.wiley.com/10.1111/lam.13104
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http://dx.doi.org/10.1111/lam.13104DOI Listing
February 2019
2 Reads

Fractionation and characterization of lignin streams from unique high-lignin content endocarp feedstocks.

Biotechnol Biofuels 2018 8;11:304. Epub 2018 Nov 8.

1Biosystems and Agricultural Engineering, University of Kentucky, Lexington, KY 40506 USA.

Background: Lignin is a promising source of building blocks for upgrading to valuable aromatic chemicals and materials. Endocarp biomass represents a non-edible crop residue in an existing agricultural setting which cannot be used as animal feed nor soil amendment. With significantly higher lignin content and bulk energy density, endocarps have significant advantages to be converted into both biofuel and bioproducts as compared to other biomass resources. Read More

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https://biotechnologyforbiofuels.biomedcentral.com/articles/
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http://dx.doi.org/10.1186/s13068-018-1305-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6222996PMC
November 2018
11 Reads

Recent Advances in the Transcriptional Regulation of Secondary Cell Wall Biosynthesis in the Woody Plants.

Front Plant Sci 2018 23;9:1535. Epub 2018 Oct 23.

Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, United States.

Plant cell walls provide structural support for growth and serve as a barrier for pathogen attack. Plant cell walls are also a source of renewable biomass for conversion to biofuels and bioproducts. Understanding plant cell wall biosynthesis and its regulation is of critical importance for the genetic modification of plant feedstocks for cost-effective biofuels and bioproducts conversion and production. Read More

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http://dx.doi.org/10.3389/fpls.2018.01535DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6206300PMC
October 2018

Short-chain ketone production by engineered polyketide synthases in Streptomyces albus.

Nat Commun 2018 11 1;9(1):4569. Epub 2018 Nov 1.

Biogical Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, California, 94720, United States.

Microbial production of fuels and commodity chemicals has been performed primarily using natural or slightly modified enzymes, which inherently limits the types of molecules that can be produced. Type I modular polyketide synthases (PKSs) are multi-domain enzymes that can produce unique and diverse molecular structures by combining particular types of catalytic domains in a specific order. This catalytic mechanism offers a wealth of engineering opportunities. Read More

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http://www.nature.com/articles/s41467-018-07040-0
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http://dx.doi.org/10.1038/s41467-018-07040-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6212451PMC
November 2018
13 Reads

Proteomic and metabolomic analysis of the cellular biomarkers related to inhibitors tolerance in ZM4.

Biotechnol Biofuels 2018 16;11:283. Epub 2018 Oct 16.

1College of Resources and Environment, University of Chinese Academy of Sciences, 19 A Yuquan Road, Shijingshan District, Beijing, 100049 People's Republic of China.

Background: Toxic compounds present in both the hydrolysate and pyrolysate of lignocellulosic biomass severely hinder the further conversion of lignocellulose-derived fermentable sugars into useful chemicals by common biocatalysts like , which has remarkable advantages over yeast. Although the extra detoxification treatment prior to fermentation process can help biocatalysts to eliminate the inhibitory environment, it is not environment friendly and cost effective for industrial application. As also reported by previous studies, an ideal and holistic approach to solve this issue is to develop microbial strains with inhibitor tolerance. Read More

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https://biotechnologyforbiofuels.biomedcentral.com/articles/
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http://dx.doi.org/10.1186/s13068-018-1287-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6190654PMC
October 2018
13 Reads

Restoration of biofuel production levels and increased tolerance under ionic liquid stress is enabled by a mutation in the essential Escherichia coli gene cydC.

Microb Cell Fact 2018 Oct 8;17(1):159. Epub 2018 Oct 8.

Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA.

Background: Microbial production of chemicals from renewable carbon sources enables a sustainable route to many bioproducts. Sugar streams, such as those derived from biomass pretreated with ionic liquids (IL), provide efficiently derived and cost-competitive starting materials. A limitation to this approach is that residual ILs in the pretreated sugar source can be inhibitory to microbial growth and impair expression of the desired biosynthetic pathway. Read More

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https://microbialcellfactories.biomedcentral.com/articles/10
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http://dx.doi.org/10.1186/s12934-018-1006-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6174563PMC
October 2018
12 Reads

Water impacts of U.S. biofuels: Insights from an assessment combining economic and biophysical models.

PLoS One 2018 28;13(9):e0204298. Epub 2018 Sep 28.

Department of Space, Earth and Environment, Chalmers University of Technology, Gothenburg, Sweden.

Biofuels policies induce land use changes (LUC), including cropland expansion and crop switching, and this in turn alters water and soil management practices. Policies differ in the extent and type of land use changes they induce and therefore in their impact on water resources. We quantify and compare the spatially varying water impacts of biofuel crops stemming from LUC induced by two different biofuels policies by coupling a biophysical model with an economic model to simulate the economically viable mix of crops, land uses, and crop management choices under alternative policy scenarios. Read More

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0204298PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6161887PMC
March 2019
11 Reads

Metabolic network reconstruction and phenome analysis of the industrial microbe, Escherichia coli BL21(DE3).

PLoS One 2018 21;13(9):e0204375. Epub 2018 Sep 21.

Department of Bioscience and Biotechnology, Konkuk University, Seoul, Republic of Korea.

Escherichia coli BL21(DE3) is an industrial model microbe for the mass-production of bioproducts such as biofuels, biorefineries, and recombinant proteins. However, despite its important role in scientific research and biotechnological applications, a high-quality metabolic network model for metabolic engineering is yet to be developed. Here, we present the comprehensive metabolic network model of E. Read More

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0204375PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6150544PMC
March 2019
9 Reads

Resource Recovery Potential From Lignocellulosic Feedstock Upon Lysis With Ionic Liquids.

Front Bioeng Biotechnol 2018 5;6:119. Epub 2018 Sep 5.

Group of Chemical and Environmental Engineering, University Rey Juan Carlos, Mostoles, Spain.

Lignocellulosic residues from energy crops offer a high potential to recover bioproducts and biofuels that can be used as raw matter for agriculture activities within a circular economy framework. Anaerobic digestion (AD) is a well-established driver to convert these residues into energy and bioproducts. However, AD of lignocellulosic matter is slow and yields low methane potential, and therefore several pre-treatment methods have been proposed to increase the energy yield of this process. Read More

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http://dx.doi.org/10.3389/fbioe.2018.00119DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6134079PMC
September 2018
2 Reads

The Shape of Native Plant Cellulose Microfibrils.

Sci Rep 2018 Sep 18;8(1):13983. Epub 2018 Sep 18.

Department of Biology, The Pennsylvania State University, University Park, PA, USA.

Determining the shape of plant cellulose microfibrils is critical for understanding plant cell wall molecular architecture and conversion of cellulose into biofuels. Only recently has it been determined that these cellulose microfibrils are composed of 18 cellulose chains rather than 36 polymers arranged in a diamond-shaped pattern. This study uses density functional theory calculations to model three possible habits for the 18-chain microfibril and compares the calculated energies, structures, C NMR chemical shifts and WAXS diffractograms of each to evaluate which shape is most probable. Read More

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http://dx.doi.org/10.1038/s41598-018-32211-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6143632PMC
September 2018
1 Read

The Alcohol-to-Jet Conversion Pathway for Drop-In Biofuels: Techno-Economic Evaluation.

ChemSusChem 2018 Nov 26;11(21):3728-3741. Epub 2018 Oct 26.

Bioproducts, Sciences, and Engineering Laboratory, Washington State University, 2710 Crimson Way, Richland, WA, 99354, USA.

The alcohol-to-jet (ATJ) process is a method for the conversion of alcohols to an alternative jet fuel blendstock based on catalytic steps historically utilized by the petroleum refining and petrochemical industry. This pathway provides a means for producing a sustainable alternative jet fuel (SAJF) from a wide variety of resources and offers a near-term opportunity for alcohol producers to enter the SAJF market and for the aviation sector to meet growing SAJF demand. Herein, the technical background is reviewed and selected variations of ATJ processes evaluated. Read More

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http://dx.doi.org/10.1002/cssc.201801690DOI Listing
November 2018

Investigation on the Cultivation Conditions of a Newly Isolated Fusarium Fungal Strain for Enhanced Lipid Production.

Authors:
Yan Yang Bo Hu

Appl Biochem Biotechnol 2019 Apr 11;187(4):1220-1237. Epub 2018 Sep 11.

Department of Bioproducts and Biosystems Engineering, University of Minnesota, 1390 Eckles Ave, Saint Paul, MN, 55108-6005, USA.

Fusarium equiseti UMN-1 fungal strain isolated from soybean is selected as a potential oleaginous fungal strain for biodiesel generation. It possesses desirable features, such as high lipid content (up to 56%) and high fatty acid methyl ester (FAME) content (more than 98%) in total lipids, and also has the capability to produce cellulase. This research focused on the investigation of the characteristics of this strain and optimization of culture conditions to enhance lipid production. Read More

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http://dx.doi.org/10.1007/s12010-018-2870-8DOI Listing

Biosynthesis of advanced biofuel farnesyl acetate using engineered Escherichia coli.

Bioresour Technol 2018 Dec 29;269:577-580. Epub 2018 Aug 29.

Key Laboratory of Fermentation Engineering (Ministry of Education), Hubei Key Laboratory of Industrial Microbiology, Hubei Collaborative Innovation Center of Industrial Fermentation, Hubei University of Technology, Wuhan 430068, China. Electronic address:

Diminishing petroleum reserves and the rapid accumulation of greenhouse gases lead to increasing interest in microbial biofuels. In this study, a heterologous farnesyl acetate biosynthesis pathway was constructed in Escherichia coli for the first time. Firstly, the AtoB, ERG13, tHMG1, ERG12, ERG8, MVD1, Idi, IspA and PgpB were expressed to accumulate farnesol in the E. Read More

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http://dx.doi.org/10.1016/j.biortech.2018.08.112DOI Listing
December 2018
1 Read

Cell-Free Synthetic Biology for Pathway Prototyping.

Methods Enzymol 2018 27;608:31-57. Epub 2018 Jun 27.

Department of Chemical and Biological Engineering, Northwestern University, Evanston, IL, United States; Chemistry of Life Processes Institute, Northwestern University, Evanston, IL, United States; Center for Synthetic Biology, Northwestern University, Evanston, IL, United States; Robert H. Lurie Comprehensive Cancer Center, Northwestern University, Chicago, IL, United States; Simpson Querrey Institute, Northwestern University, Chicago, IL, United States. Electronic address:

Engineering biological systems for the production of biofuels and bioproducts holds great potential to transform the bioeconomy, but often requires laborious, time-consuming design-build-test cycles. For decades cell-free systems have offered quick and facile approaches to study enzymes with hopes of informing cellular processes, mainly in the form of purified single-enzyme activity assays. Over the past 20 years, cell-free systems have grown to include multienzymatic systems, both purified and crude. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00766879183018
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http://dx.doi.org/10.1016/bs.mie.2018.04.029DOI Listing
June 2018
7 Reads

Production of bio-oil from agricultural waste by using a continuous fast microwave pyrolysis system.

Bioresour Technol 2018 Dec 18;269:162-168. Epub 2018 Aug 18.

Nanchang University, State Key Laboratory of Food Science and Technology, Nanchang 330047, China; Nanchang University, Engineering Research Center for Biomass Conversion, Ministry of Education, Nanchang 330047, China; Center for Biorefining and Department of Bioproducts and Biosystems Engineering, University of Minnesota, 1390 Eckles Ave., St. Paul, MN 55108, USA.

In this study, a continuous fast microwave-assisted pyrolysis system was developed to produce bio-oil, gas, and biochar from rice straw and Camellia oleifera shell. The effects of different pyrolysis temperatures (400 °C, 500 °C, and 600 °C) and feed rates (rice straw: 25, 45, and 66 g/min; C. oleifera shell: 100, 200, and 400 g/min) on bio-oil production were investigated. Read More

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http://dx.doi.org/10.1016/j.biortech.2018.08.067DOI Listing
December 2018
15 Reads

High-value biomass from microalgae production platforms: strategies and progress based on carbon metabolism and energy conversion.

Biotechnol Biofuels 2018 20;11:227. Epub 2018 Aug 20.

1Institute for Food & Bioresource Engineering, College of Engineering, Peking University, Beijing, 100871 China.

Microalgae are capable of producing sustainable bioproducts and biofuels by using carbon dioxide or other carbon substances in various cultivation modes. It is of great significance to exploit microalgae for the economical viability of biofuels and the revenues from high-value bioproducts. However, the industrial performance of microalgae is still challenged with potential conflict between cost of microalgae cultivation and revenues from them, which is mainly ascribed to the lack of comprehensive understanding of carbon metabolism and energy conversion. Read More

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http://dx.doi.org/10.1186/s13068-018-1225-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6100726PMC
August 2018
2 Reads
1 Citation
6.044 Impact Factor

Removal of non-structural components from poplar whole-tree chips to enhance hydrolysis and fermentation performance.

Biotechnol Biofuels 2018 11;11:222. Epub 2018 Aug 11.

Biofuels and Bioproducts Laboratory, School of Environmental and Forest Sciences, University of Washington, Box 352100, Seattle, WA 98195-2100 USA.

Background: Whole-tree chips will be a likely feedstock for future biorefineries because of their low cost. Non-structural components (NSC), however, represent a significant part of whole-tree chips. The NSC can account for more than 10% of whole-tree poplar mass when the trees are grown in short rotation cycles. Read More

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http://dx.doi.org/10.1186/s13068-018-1219-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6086995PMC
August 2018
3 Reads

The growing U.S. bioeconomy: Drivers, development and constraints.

N Biotechnol 2019 Mar 16;49:48-57. Epub 2018 Aug 16.

Department of Chemistry, Delaware State University, Dover, DE 19901, USA.

Bioeconomy refers to economic activities using renewable biological resources to produce energy and domestic consumables. The U.S. Read More

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http://dx.doi.org/10.1016/j.nbt.2018.08.005DOI Listing
March 2019
1 Read

Start-up of a microalgae-based treatment system within the biorefinery concept: from wastewater to bioproducts.

Water Sci Technol 2018 Aug;78(1-2):114-124

GEMMA - Environmental Engineering and Microbiology Research Group, Department of Civil and Environmental Engineering, Universitat Politècnica de Catalunya-BarcelonaTech, c/ Jordi Girona 1-3, Building D1, Barcelona E-08034, Spain E-mail:

Within the European project INCOVER, an experimental microalgae-based treatment system has been built for wastewater reuse and added-value products generation. This article describes this new experimental plant and the start-up stage, starting from the new design of three semi-closed horizontal photobioreactors with low energy requirements for microalgae cultivation (30 m total), using agricultural runoff and urban wastewater as feedstock. The inflow nutrients concentration is adjusted to select cyanobacteria, microalgae able to accumulate polyhydroxybutyrates, which can be used for bioplastics production. Read More

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http://wst.iwaponline.com/lookup/doi/10.2166/wst.2018.195
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http://dx.doi.org/10.2166/wst.2018.195DOI Listing
August 2018
5 Reads

Effective one-step saccharification of lignocellulosic biomass using magnetite-biocatalysts containing saccharifying enzymes.

Sci Total Environ 2019 Jan 5;647:806-813. Epub 2018 Aug 5.

Zachry Department of Civil Engineering, Texas A&M University, College Station, TX 77843-3136, USA. Electronic address:

Lignocellulosic biomass, packed with sugars, is one of the most available renewable resources for biofuels and bioproducts production. To release the sugars for the production, enzymatic hydrolysis (saccharification) of pretreated lignocellulosic biomass are required. However, the saccharification process is costly, inefficient, and requires multi-step operations. Read More

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http://dx.doi.org/10.1016/j.scitotenv.2018.08.066DOI Listing
January 2019
13 Reads

Improving hydrocarbon yield via catalytic fast co-pyrolysis of biomass and plastic over ceria and HZSM-5: An analytical pyrolyzer analysis.

Bioresour Technol 2018 Nov 21;268:1-8. Epub 2018 Jul 21.

Center for Biorefining and Department of Bioproducts and Biosystems Engineering, University of Minnesota, 1390 Eckles Ave, St. Paul, MN 55108, United States; Engineering Research Center for Biomass Conversion, Ministry of Education, Nanchang University, Nanchang 330047, China. Electronic address:

The excessive oxygen content in biomass obstructs the production of high-quality bio-oils. In this work, we developed a tandem catalytic bed (TCB) of CeO and HZSM-5 in an analytical pyrolyzer to enhance the hydrocarbon production from co-pyrolysis of corn stover (CS) and LDPE. Results indicated that CeO could remove oxygen from acids, aldehydes and methoxy phenols, producing a maximum yield of hydrocarbons of 85% and highest selectivity of monocyclic aromatics of 73% in the TCB. Read More

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http://dx.doi.org/10.1016/j.biortech.2018.07.108DOI Listing
November 2018
8 Reads

In-situ and ex-situ catalytic upgrading of vapors from microwave-assisted pyrolysis of lignin.

Bioresour Technol 2018 Jan 3;247:851-858. Epub 2017 Oct 3.

Center for Biorefining and Department of Bioproducts and Biosystems Engineering, University of Minnesota, 1390 Eckles Ave., St. Paul, MN 55108, United States. Electronic address:

In-situ and ex-situ catalytic upgrading with HZSM-5 of vapors from microwave-assisted pyrolysis of lignin were studied. The in-situ process produced higher bio-oil and less char than ex-situ process. The gas yield was similar for both processes. Read More

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http://dx.doi.org/10.1016/j.biortech.2017.09.200DOI Listing
January 2018
2 Reads

Cultivation of Chlorella vulgaris on anaerobically digested swine manure with daily recycling of the post-harvest culture broth.

Bioresour Technol 2018 Jan 27;247:716-723. Epub 2017 Sep 27.

Center for Biorefining and Department of Bioproducts and Biosystems Engineering, University of Minnesota, 1390 Eckles Avenue, St. Paul, MN 55108, United States; The Engineering Research Center for Biomass Conversion, Ministry of Education, Nanchang University, Jiangxi 330047, People's Republic of China. Electronic address:

In this work, a cultivation system with daily recycling of the post-harvest culture broth was set up and performed in order to reuse the water and nutrients in pretreated anaerobically digested swine manure, which was used as media to cultivate Chlorella vulgaris (UTEX 2714) at different recycling ratios. Results showed that the alga grew well in the system with an accumulative algal biomass and productivity of 1.68-3. Read More

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http://dx.doi.org/10.1016/j.biortech.2017.09.171DOI Listing
January 2018
2 Reads

Industrial potato peel as a feedstock for biobutanol production.

N Biotechnol 2018 Nov 22;46:54-60. Epub 2018 Jul 22.

Biofuels and Bioproducts Research Centre, Institute of Agricultural Technology of Castile and Leon (ITACyL), Villarejo de Órbigo, E-24358 León, Spain; Chemical and Environmental Bioprocess Engineering, Institute of Natural Resources (IRENA), University of Leon, Avenida de Portugal 42, E-24071 León, Spain. Electronic address:

Potato peel from a snack factory was assessed as possible feedstock for biobutanol production. This lignocellulosic biomass was subjected to various physicochemical pretreatments (autohydrolysis and hydrolysis with dilute acids, alkalis, organic solvents or surfactants) under different conditions of time, temperature and reagent concentrations, in order to favour the release of sugars and reduce the generation of fermentation inhibitors. Thereafter, the pretreated potato peel was treated enzymatically to complete the hydrolysis. Read More

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http://dx.doi.org/10.1016/j.nbt.2018.07.002DOI Listing
November 2018
11 Reads

Increasing the economic value of lignocellulosic stillage through medium-chain fatty acid production.

Biotechnol Biofuels 2018 19;11:200. Epub 2018 Jul 19.

1The Great Lakes Bioenergy Research Center, University of Wisconsin-Madison, Madison, WI USA.

Background: Lignocellulosic biomass is seen as an abundant renewable source of liquid fuels and chemicals that are currently derived from petroleum. When lignocellulosic biomass is used for ethanol production, the resulting liquid residue (stillage) contains large amounts of organic material that could be further transformed into recoverable bioproducts, thus enhancing the economics of the biorefinery.

Results: Here we test the hypothesis that a bacterial community could transform the organics in stillage into valuable bioproducts. Read More

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http://dx.doi.org/10.1186/s13068-018-1193-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6052542PMC
July 2018
9 Reads

Dual Utilization of Medicinal and Aromatic Crops as Bioenergy Feedstocks.

J Agric Food Chem 2018 Aug 13;66(33):8744-8752. Epub 2018 Aug 13.

Department of Plant Sciences , University of Tennessee , Knoxville , Tennessee 37996 , United States.

Dual production of biofuels and chemicals can increase the economic value of lignocellulosic bioenergy feedstocks. We compared the bioenergy potential of several essential oil (EO) crops with switchgrass ( Panicum virgatum L.), a crop chosen to benchmark biomass and lignocellulosic biofuel production. Read More

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http://dx.doi.org/10.1021/acs.jafc.7b04594DOI Listing

Silicon carbide foam supported ZSM-5 composite catalyst for microwave-assisted pyrolysis of biomass.

Bioresour Technol 2018 Nov 4;267:257-264. Epub 2018 Jul 4.

Center for Biorefining and Department of Bioproducts and Biosystems Engineering, University of Minnesota, 1390 Eckles Ave., St. Paul, MN 55108, USA; Ministry of Education Engineering Research Center for Biomass Conversion, Nanchang University, 235 Nanjing Road, Nanchang, Jiangxi 330047, China. Electronic address:

Considering a series of issues facing the application of catalysts in large scale catalytic fast pyrolysis systems, a novel composite catalyst of ZSM-5 coatings on SiC foam supports was developed and tested for ex-situ catalytic upgrading of the pyrolytic vapors. Different configurations of catalysts placement were compared and the results showed the composite catalyst could significantly improve the bio-oil quality without significantly reducing the yield. The effect of catalyst to biomass ratio on the product yields and bio-oil composition was studied and the results showed that increasing catalyst to biomass ratio could improve the quality of bio-oil at the cost of its yield. Read More

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http://dx.doi.org/10.1016/j.biortech.2018.07.007DOI Listing
November 2018
12 Reads

Novel sequencing technologies to support industrial biotechnology.

FEMS Microbiol Lett 2018 Aug;365(16)

Bioinformatics Department, BaseClear B.V., Sylviusweg 74, 2333 BE, Leiden, The Netherlands.

Industrial biotechnology develops and applies microorganisms for the production of bioproducts and enzymes with applications ranging from food and feed ingredients and processing to bio-based chemicals, biofuels and pharmaceutical products. Next generation DNA sequencing technologies play an increasingly important role in improving and accelerating microbial strain development for existing and novel bio-products via screening, gene and pathway discovery, metabolic engineering and additional optimization and understanding of large-scale manufacturing. In this mini-review, we describe novel DNA sequencing and analysis technologies with a focus on applications to industrial strain development, enzyme discovery and microbial community analysis. Read More

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http://dx.doi.org/10.1093/femsle/fny103DOI Listing
August 2018
10 Reads

Vortex fluidic mediated direct transesterification of wet microalgae biomass to biodiesel.

Bioresour Technol 2018 Oct 5;266:488-497. Epub 2018 Jul 5.

Centre for Marine Bioproducts Development, Flinders University, South Australia 5042, Australia; Medical Biotechnology, College of Medicine and Public Health, Flinders University, South Australia 5042, Australia.

A bottleneck in the production of biodiesel from microalgae is the dewatering and lipid extraction process which is the dominant energy penalty and cost. A novel biodiesel production platform based on vortex fluidic device (VFD)-assisted direct transesterification (DT) of wet microalgal biomass of Chloroparva pannonica was developed and evaluated. Fatty acid extraction and fatty acid to FAME conversion efficiencies were used at different parameter settings to evaluate performance of the processing technology in confined and continuous mode. Read More

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http://dx.doi.org/10.1016/j.biortech.2018.06.103DOI Listing
October 2018
17 Reads

Hybrid phenolic-inducible promoters towards construction of self-inducible systems for microbial lignin valorization.

Biotechnol Biofuels 2018 28;11:182. Epub 2018 Jun 28.

1Biomass Science and Conversion Technology Department, Sandia National Laboratories, Livermore, CA USA 94550.

Background: Engineering strategies to create promoters that are both higher strength and tunable in the presence of inexpensive compounds are of high importance to develop metabolic engineering technologies that can be commercialized. Lignocellulosic biomass stands out as the most abundant renewable feedstock for the production of biofuels and chemicals. However, lignin a major polymeric component of the biomass is made up of aromatic units and remains as an untapped resource. Read More

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http://dx.doi.org/10.1186/s13068-018-1179-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6022352PMC
June 2018
18 Reads
6.040 Impact Factor

Towards a sustainable bio-based economy: Redirecting primary metabolism to new products with plant synthetic biology.

Authors:
Patrick M Shih

Plant Sci 2018 Aug 14;273:84-91. Epub 2018 Mar 14.

Joint BioEnergy Institute, 5885 Hollis St, Emeryville, CA, 94608, United States; Environmental Genomics and Systems Biology Division, Lawrence Berkeley National Laboratory, One Cyclotron Rd, Berkeley, CA, 94720, United States; Department of Chemical Engineering, Stanford University, Stanford, CA, 94305, United States. Electronic address:

Humans have domesticated many plant species as indispensable sources of food, materials, and medicines. The dawning era of synthetic biology represents a means to further refine, redesign, and engineer crops to meet various societal and industrial needs. Current and future endeavors will utilize plants as the foundation of a bio-based economy through the photosynthetic production of carbohydrate feedstocks for the microbial fermentation of biofuels and bioproducts, with the end goal of decreasing our dependence on petrochemicals. Read More

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http://dx.doi.org/10.1016/j.plantsci.2018.03.012DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6005202PMC
August 2018
16 Reads

Co-pyrolysis of microwave-assisted acid pretreated bamboo sawdust and soapstock.

Bioresour Technol 2018 Oct 30;265:33-38. Epub 2018 May 30.

Nanchang University, State Key Laboratory of Food Science and Technology, Nanchang 330047, China; Nanchang University, Engineering Research Center for Biomass Conversion, Ministry of Education, Nanchang 330047, China.

Fast microwave-assisted co-pyrolysis of pretreated bamboo sawdust and soapstock was conducted. The pretreatment process was carried out under microwave irradiation. The effects of microwave irradiation temperature, irradiation time, and concentration of hydrochloric acid on product distribution from co-pyrolysis and the relative contents of the major components in bio-oil were investigated. Read More

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http://dx.doi.org/10.1016/j.biortech.2018.05.095DOI Listing
October 2018
24 Reads

The spatial proximity effect of beta-glucosidase and cellulosomes on cellulose degradation.

Enzyme Microb Technol 2018 Aug 1;115:52-61. Epub 2018 May 1.

Shandong Provincial Key Laboratory of Energy Genetics, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, PR China; Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, PR China; Qingdao Engineering Laboratory of Single Cell Oil, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, PR China. Electronic address:

Low-cost saccharification is one of the key bottlenecks hampering the further application of lignocellulosic biomass. Clostridium thermocellum is a naturally ideal cellulose degrading bacterium armed with cellulosomes, which are multienzyme complexes that are capable of efficiently degrading cellulose. However, under controlled condition, the inhibition effect of hydrolysate cellobiose severely restricts the hydrolytic ability of cellulosomes. Read More

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http://dx.doi.org/10.1016/j.enzmictec.2018.04.009DOI Listing
August 2018
5 Reads