13,710 results match your criteria Biofuels [Journal]


Tools and strategies for constructing cell-free enzyme pathways.

Biotechnol Adv 2018 Dec 3. Epub 2018 Dec 3.

Department of Molecular Sciences, Macquarie University, Sydney, Australia; Biomolecular Discovery and Design Research Centre, Macquarie University, Sydney, Australia. Electronic address:

Single enzyme systems or engineered microbial hosts have been used for decades but the notion of assembling multiple enzymes into cell-free synthetic pathways is a relatively new development. The extensive possibilities that stem from this synthetic concept makes it a fast growing and potentially high impact field for biomanufacturing fine and platform chemicals, pharmaceuticals and biofuels. However, the translation of individual single enzymatic reactions into cell-free multi-enzyme pathways is not trivial. Read More

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December 2018
1 Read

Simultaneous consumption of cellobiose and xylose by to circumvent glucose repression and identification of its cellobiose-assimilating operons.

Biotechnol Biofuels 2018 1;11:320. Epub 2018 Dec 1.

1Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, Nanjing Forestry University, Nanjing, 210037 People's Republic of China.

Background: The use of inedible lignocellulosic biomasses for biomanufacturing provides important environmental and economic benefits for society. Efficient co-utilization of lignocellulosic biomass-derived sugars, primarily glucose and xylose, is critical for the viability of lignocellulosic biorefineries. However, the phenomenon of glucose repression prevents co-utilization of both glucose and xylose in cellulosic hydrolysates. Read More

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December 2018

Cellulose-specific Type B carbohydrate binding modules: understanding oligomeric and non-crystalline substrate recognition mechanisms.

Biotechnol Biofuels 2018 30;11:319. Epub 2018 Nov 30.

Department of Chemical and Materials Engineering, University of Kentucky, 177 F Paul Anderson Tower, Lexington, KY 40506 USA.

Background: Effective enzymatic degradation of crystalline polysaccharides requires a synergistic cocktail of hydrolytic enzymes tailored to the wide-ranging degree of substrate crystallinity. To accomplish this type of targeted carbohydrate recognition, nature produces multi-modular enzymes, having at least one catalytic domain appended to one or more carbohydrate binding modules (CBMs). The Type B CBM categorization encompasses several families (i. Read More

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November 2018

Oxidation stability of yeast biodiesel using Rancimat analysis: validation using infrared spectroscopy and gas chromatography-mass spectrometry.

Environ Sci Pollut Res Int 2018 Dec 1. Epub 2018 Dec 1.

Government College of Engineering, Bodinayakkanur, Tamil Nadu, India.

Biodiesel and single cell oils obtained from oleaginous yeasts grown in industrial waste are attractive alternatives to the conventional fuels. However, there are only few articles dealing with the stability of the microbial biofuels. Hence, this study aimed at characterizing the storage time of biodiesels using Rancimat methods. Read More

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December 2018

Fatty acid biosynthesis is enhanced in Escherichia coli strains with deletion in genes encoding the PII signaling proteins.

Arch Microbiol 2018 Nov 30. Epub 2018 Nov 30.

Department of Biochemistry and Molecular Biology, UFPR, Curitiba, PR, Brazil.

The committed and rate-limiting step in fatty acid biosynthesis is catalyzed by acetyl-CoA carboxylase (ACC). In previous studies we showed that ACC activity is inhibited through interactions with the PII signaling proteins in vitro. Here we provide in vivo support for that model; we noted that PII proteins are able to reduce malonyl-CoA levels in vivo in Escherichia coli. Read More

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November 2018

Biotechnological applications of inulin-rich feedstocks.

Bioresour Technol 2018 Nov 9. Epub 2018 Nov 9.

Université Clermont Auvergne, Institut Pascal, UMR, CNRS 6602, and Labex, IMobS3, 4 Avenue Blaise Pascal, TSA 60026, CS 60026, F-63178 Aubiere Cedex, France.

Inulin is a naturally occurring second largest storage polysaccharide with a wide range of applications in pharmaceutical and food industries. It is a robust polysaccharide which consists of a linear chain of β-2, 1-linked-d-fructofuranose molecules terminated with α-d-glucose moiety at the reducing end. It is present in tubers, bulbs and tuberous roots of more than 36,000 plants belonging to both monocotyledonous and dicotyledonous families. Read More

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November 2018
3 Reads

Impacts of maricultural activities on characteristics of dissolved organic carbon and nutrients in a typical raft-culture area of the Yellow Sea, North China.

Mar Pollut Bull 2018 Dec 27;137:456-464. Epub 2018 Oct 27.

State Key Laboratory for Marine Environmental Science, Xiamen University, Xiamen 361100, China.

Ailian Bay is an integrated multi-trophic aquaculture bay with approximately 60 years maricultural activities in North China. The floating raft culture of kelp and shellfish is the unique mariculture mode. In this study, the impacts of intensive mariculture activities on seasonal carbon and nutrient dynamics in Ailian Bay were systematically analysed via seasonal surveys between 2015 and 2016. Read More

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December 2018

Comparative assessment on the extraction of carotenoids from microalgal sources: Astaxanthin from H. pluvialis and β-carotene from D. salina.

Food Chem 2019 Mar 13;277:128-134. Epub 2018 Oct 13.

Department of Mechanical Engineering, University of Moratuwa, Sri Lanka. Electronic address:

Astaxanthin and β-carotene are important carotenoids used in numerous pharmaceutical and nutraceutical applications, owing to their vigorous antioxidant properties. The microalgal strains Haematococcus pluvialis and Dunaliella salina accumulate the highest quantities of astaxanthin and β-carotene (up to 7% and 13% dry weight respectively) and are therefore considered as sustainable feedstock for the commercial production of carotenoids. Thus, from an economical perspective, it becomes desirable to optimize recovery of carotenoids from microalgal cells. Read More

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Fibre degradation of wheat straw by Pleurotus erygnii under low moisture conditions during solid state fermentation.

Lett Appl Microbiol 2018 Dec 5. Epub 2018 Dec 5.

School of Environment, Natural Resources and Geography, Bangor University, Bangor, Gwynedd, LL57 2UW, UK.

The application of solid state fermentation offers an alternative to conventional, submerged approaches for a variety of bioconversion processes, including animal feeds, biofuels and fungal bioproducts. Optimising solid state fermentation under low moisture conditions could significantly impact the proportion of dry biomass that could be processed and improve the commercial viability of this approach, because of reduced input costs and higher yields of final products. Pleurotus erygnii that appeared to show tolerance to low moisture conditions was grown on saturated and desaturated wheat straw. Read More

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December 2018

Selectable marker recycling in the nonconventional yeast Xanthophyllomyces dendrorhous by transient expression of Cre on a genetically unstable vector.

Appl Microbiol Biotechnol 2018 Dec 4. Epub 2018 Dec 4.

Shandong Provincial Key Laboratory of Synthetic Biology, Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, No.189 Songling Road, Laoshan District, Qingdao, 266101, Shandong Province, China.

Selectable marker recycling is a basic technique in bioengineering. However, this technique is usually unavailable in non-model microorganisms. In this study, we proposed a simple and efficient method for selectable marker recycling in the astaxanthin-synthesizing yeast Xanthophyllomyces dendrorhous. Read More

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December 2018

An integrated omics analysis reveals molecular mechanisms that are associated with differences in seed oil content between Glycine max and Brassica napus.

BMC Plant Biol 2018 Dec 4;18(1):328. Epub 2018 Dec 4.

Crop Information Center, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China.

Background: Rapeseed (Brassica napus L.) and soybean (Glycine max L.) seeds are rich in both protein and oil, which are major sources of biofuels and nutrition. Read More

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December 2018

Structures of endo-1,5-α-L-arabinanase mutants from Bacillus thermodenitrificans TS-3 in complex with arabino-oligosaccharides.

Acta Crystallogr F Struct Biol Commun 2018 Dec 26;74(Pt 12):774-780. Epub 2018 Nov 26.

Graduate School of Science, Osaka Prefecture University, Sakai, Osaka 599-8531, Japan.

The thermostable endo-1,5-α-L-arabinanase from Bacillus thermodenitrificans TS-3 (ABN-TS) hydrolyzes the α-1,5-L-arabinofuranoside linkages of arabinan. In this study, the crystal structures of inactive ABN-TS mutants, D27A and D147N, were determined in complex with arabino-oligosaccharides. The crystal structures revealed that ABN-TS has at least six subsites in the deep V-shaped cleft formed across one face of the propeller structure. Read More

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December 2018
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Bioethanol Production from Renewable Raw Materials and Its Separation and Purification: A Review.

Food Technol Biotechnol 2018 Sep;56(3):289-311

University of Zagreb, Faculty of Food Technology and Biotechnology, Pierottijeva 6, HR-10000 Zagreb, Croatia.

Production of biofuels from renewable feedstocks has captured considerable scientific attention since they could be used to supply energy and alternative fuels. Bioethanol is one of the most interesting biofuels due to its positive impact on the environment. Currently, it is mostly produced from sugar- and starch-containing raw materials. Read More

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September 2018
2 Reads

Production and spectroscopic characterization of lytic polysaccharide monooxygenases.

Methods Enzymol 2018 15;613:63-90. Epub 2018 Nov 15.

Department of Chemistry, University of York, York, United Kingdom. Electronic address:

Lytic polysaccharide monooxygenases (LPMOs, also known as PMOs) are a recently discovered family of enzymes that play a key role in the breakdown of polysaccharide substrates. The ability of LPMOs to introduce chain breaks, using an oxidative mechanism, has particularly attracted attention as the world seeks more cost-effective and environmentally friendly ways of producing second-generation biofuels for the future. LPMOs are copper-dependent enzymes and have an unusual active site which includes the N-terminal residue of the protein in the copper coordination sphere. Read More

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November 2018

New horizons in culture and valorization of red microalgae.

Biotechnol Adv 2018 Nov 27. Epub 2018 Nov 27.

CNRS, SIGMA Clermont, Institut Pascal, Université Clermont Auvergne, F-63000 Clermont-Ferrand, France. Electronic address:

Research on marine microalgae has been abundantly published and patented these last years leading to the production and/or the characterization of some biomolecules such as pigments, proteins, enzymes, biofuels, polyunsaturated fatty acids, enzymes and hydrocolloids. This literature focusing on metabolic pathways, structural characterization of biomolecules, taxonomy, optimization of culture conditions, biorefinery and downstream process is often optimistic considering the valorization of these biocompounds. However, the accumulation of knowledge associated with the development of processes and technologies for biomass production and its treatment has sometimes led to success in the commercial arena. Read More

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November 2018

Evolutionary engineered Candida intermedia exhibits improved xylose utilization and robustness to lignocellulose-derived inhibitors and ethanol.

Appl Microbiol Biotechnol 2018 Nov 29. Epub 2018 Nov 29.

Department of Biology and Biological Engineering, Division of Industrial Biotechnology, Chalmers University of Technology, 41296, Gothenburg, Sweden.

The development of robust microorganisms that can efficiently ferment both glucose and xylose represents one of the major challenges in achieving a cost-effective lignocellulosic bioethanol production. Candida intermedia is a non-conventional, xylose-utilizing yeast species with a high-capacity xylose transport system. The natural ability of C. Read More

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November 2018

Synthetic Biology Tools to Engineer Microbial Communities for Biotechnology.

Trends Biotechnol 2018 Nov 26. Epub 2018 Nov 26.

Imperial College Centre for Synthetic Biology, Imperial College London, London SW7 2AZ, UK; Department of Bioengineering, Imperial College London, London SW7 2AZ, UK. Electronic address:

Microbial consortia have been used in biotechnology processes, including fermentation, waste treatment, and agriculture, for millennia. Today, synthetic biologists are increasingly engineering microbial consortia for diverse applications, including the bioproduction of medicines, biofuels, and biomaterials from inexpensive carbon sources. An improved understanding of natural microbial ecosystems, and the development of new tools to construct synthetic consortia and program their behaviors, will vastly expand the functions that can be performed by communities of interacting microorganisms. Read More

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November 2018

Heterologous expression and biochemical characterization of a novel cold-active α-amylase from the Antarctic bacteria Pseudoalteromonas sp. 2-3.

Protein Expr Purif 2018 Nov 27;155:78-85. Epub 2018 Nov 27.

Centre for Biotechnology and Bioengineering (CeBiB), Department of Chemical Engineering, Biotechnology and Materials, University of Chile, Beauchef 851, Santiago, Chile. Electronic address:

α-Amylase is an endo-acting enzyme which catalyzes random hydrolysis of starch. These enzymes are used in various biotechnological processes including the textile, paper, food, biofuels, detergents and pharmaceutical industries. The use of active enzymes at low temperatures has a high potential because these enzymes would avoid the demand for heating during the process thereby reducing costs. Read More

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November 2018

Effects of limonene, n-decane and n-decanol on growth and membrane fatty acid composition of the microalga Botryococcus braunii.

AMB Express 2018 Nov 28;8(1):189. Epub 2018 Nov 28.

Department of Chemical Engineering, Centre for Biotechnology and Bioengineering (CeBiB), Scientific and Technological Bio-resource Nucleus, University of La Frontera, Av. Francisco Salazar 01145, Temuco, Chile.

Botryococcus braunii is a promising microalga for the production of biofuels and other chemicals because of its high content of internal lipids and external hydrocarbons. However, due to the very thick cell wall of B. braunii, traditional chemical/physical downstream processing very often is not as effective as expected and requires high amounts of energy. Read More

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November 2018

Impact of Nutrient Availability on Tertiary Wastewater Treatment by .

Water Environ Res 2018 Nov;90(11):2008-2016

Algae can use excessive nutrients from wastewater effluent to generate beneficial products such as biofuels. However, fluctuation of wastewater characteristics could hinder the implementation of tertiary algal treatment. This study aims to identify the impact of nutrient availability on 's ability to remove nitrogen and phosphorus from wastewater, and its potential as a biofuel feedstock. Read More

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November 2018

Improved methane elimination by methane-oxidizing bacteria immobilized on modified oil shale semicoke.

Sci Total Environ 2018 Nov 22;655:915-923. Epub 2018 Nov 22.

Faculty of Engineering, Qingdao University of Science and Technology, Qingdao, Shandong 266042, PR China.

Methane is a greenhouse gas with significant global warming potential. The methane-oxidizing bacteria (MOB) immobilized on biocarrier could perform effectively and environmentally in methane elimination. To further improve the efficiencies of MOB immobilization and methane elimination, the surface biocompatibility of biocarrier needs to be improved. Read More

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November 2018
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Dual expression of plastidial GPAT1 and LPAT1 regulates triacylglycerol production and the fatty acid profile in .

Biotechnol Biofuels 2018 22;11:318. Epub 2018 Nov 22.

1Key Laboratory of Eutrophication and Red Tide Prevention of Guangdong Higher Education Institutes, College of Life Science, Jinan University, Guangzhou, 510632 China.

Background: Metabolic engineering has emerged as a potential strategy for improving microalgal lipid content through targeted changes to lipid metabolic networks. However, the intricate nature of lipogenesis has impeded metabolic engineering. Therefore, it is very important to identify the crucial metabolic nodes and develop strategies to exploit multiple genes for transgenesis. Read More

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November 2018
2 Reads

Real-time adsorption and action of expansin on cellulose.

Biotechnol Biofuels 2018 22;11:317. Epub 2018 Nov 22.

1State Key Laboratory of Chemical Engineering, Tianjin Key Laboratory of Membrane Science and Desalination Technology, School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072 China.

Background: Biological pretreatment is an environmentally safe method for disrupting recalcitrant structures of lignocellulose and thereby improving their hydrolysis efficiency. Expansin and expansin-like proteins act synergistically with cellulases during hydrolysis. A systematic analysis of the adsorption behavior and mechanism of action of expansin family proteins can provide a basis for the development of highly efficient pretreatment methods for cellulosic substrates using expansins. Read More

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November 2018
4 Reads

Developing fast enzyme recycling strategy through elucidating enzyme adsorption kinetics on alkali and acid pretreated corn stover.

Biotechnol Biofuels 2018 20;11:316. Epub 2018 Nov 20.

School of Environmental and Biological Engineering, Nanjing University of Science and Technology, 200 Xiaolingwei Street, Nanjing, 210094 China.

Background: Although various pre-treatment methods have been developed to disrupt the structure of lignocellulosic biomass, high dosage of cellulases is still required to hydrolyze lignocellulose to fermentable sugars. Enzyme recycling via recycling unhydrolyzed solids after enzymatic hydrolysis is a promising strategy to reduce enzyme loading for production of cellulosic ethanol.

Results: To develop effective enzyme recycling method via recycling unhydrolyzed solids, this work investigated both enzymatic hydrolysis kinetics and enzyme adsorption kinetics on dilute acid and dilute alkali pre-treated corn stover (CS). Read More

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November 2018

grows vegetatively in a biofilm rich in heteropolysaccharides and cytoplasmic proteins.

Biotechnol Biofuels 2018 20;11:315. Epub 2018 Nov 20.

1State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, No. 30, Puzhu South Road, Nanjing, 211800 China.

Background: Biofilms are cell communities wherein cells are embedded in a self-produced extracellular polymeric substances (EPS). The biofilm of confers the cells superior phenotypes and has been extensively exploited to produce a variety of liquid biofuels and bulk chemicals. However, little has been known about the physiology of in biofilm as well as the composition and biosynthesis of the EPS. Read More

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November 2018
2 Reads

Hydrothermal liquefaction of organic resources in biotechnology: how does it work and what can be achieved?

Appl Microbiol Biotechnol 2018 Nov 24. Epub 2018 Nov 24.

SINTEF Energy Research, Postboks 4761, Torgarden, 7465, Trondheim, Norway.

Increasing the overall carbon and energy efficiency by integration of thermal processes with biological ones has gained considerable attention lately, especially within biorefining. A technology that is capable of processing wet feedstock with good energy efficiency is advantageous. Such a technology, exploiting the special properties of hot compressed water is called hydrothermal liquefaction. Read More

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November 2018
6 Reads

The putative β-glucosidase BGL3I regulates cellulase induction in .

Biotechnol Biofuels 2018 19;11:314. Epub 2018 Nov 19.

1CAS-Key Laboratory of Synthetic Biology, CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 300 Fenglin Rd, Shanghai, 200032 China.

Background: The filamentous fungus (anamorph of ) displays increased cellulase expression while growing on inducers such as lactose or cellulose. However, the mechanism of cellulase induction in is not yet completely characterized. Here, a protein annotated as β-glucosidase (BGL3I) was found to be involved in cellulase induction in . Read More

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November 2018

Retraction Note to: Bacterial conversion of depolymerized Kraft lignin.

Biotechnol Biofuels 2018 12;11:313. Epub 2018 Nov 12.

1Department of Chemical Engineering, Lund University, P.O. Box 124, 221 00 Lund, Sweden.

[This retracts the article DOI: 10.1186/s13068-018-1240-7.]. Read More

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November 2018

Yeast chemogenomic screen identifies distinct metabolic pathways required to tolerate exposure to phenolic fermentation inhibitors ferulic acid, 4-hydroxybenzoic acid and coniferyl aldehyde.

Metab Eng 2018 Nov 22;52:98-109. Epub 2018 Nov 22.

Ottawa Institute of Systems Biology, Department of Biochemistry, Microbiology and Immunology, University of Ottawa, 451 Smyth Road, Ottawa, Ontario, Canada K1H 8M5. Electronic address:

The conversion of plant material into biofuels and high value products is a two-step process of hydrolysing plant lignocellulose and next fermenting the sugars produced. However, lignocellulosic hydrolysis not only frees sugars for fermentation it simultaneously generates toxic chemicals, including phenolic compounds which severely inhibit yeast fermentation. To understand the molecular basis of phenolic compound toxicity, we performed genome-wide chemogenomic screens in Saccharomyces cerevisiae to identify deletion mutants that were either hypersensitive or resistant to three common phenolic compounds found in plant hydrolysates: coniferyl aldehyde, ferulic acid and 4-hydroxybenzoic acid. Read More

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November 2018
3 Reads

Emerging techniques for cell disruption and extraction of valuable bio-molecules of microalgae Nannochloropsis sp.

Bioprocess Biosyst Eng 2018 Nov 23. Epub 2018 Nov 23.

Laboratoire de Transformations Intégrées de la Matière Renouvelable, EA 4297, Centre de Recherches de Royallieu, Sorbonne Universités, Université de Technologie de Compiègne, BP 20529, 60205, Compiègne Cedex, France.

Microalgae of Nannochloropsis sp. present valuable source of bio-molecules (pigments, lipids, proteins) that have nutritional potential for the prevention and treatment of human diseases. Moreover, some species of Nannochloropsis are the promising sources of biofuels and excellent candidates for the replacement of classical biofuel crops. Read More

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November 2018
6 Reads

Genome-scale analysis of syngas fermenting acetogenic bacteria reveals the translational regulation for its autotrophic growth.

BMC Genomics 2018 Nov 23;19(1):837. Epub 2018 Nov 23.

Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon, 34141, Republic of Korea.

Background: Acetogenic bacteria constitute promising biocatalysts for the conversion of CO/H or synthesis gas (H/CO/CO) into biofuels and value-added biochemicals. These microorganisms are naturally capable of autotrophic growth via unique acetogenesis metabolism. Despite their biosynthetic potential for commercial applications, a systemic understanding of the transcriptional and translational regulation of the acetogenesis metabolism remains unclear. Read More

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November 2018
2 Reads

Miscanthus NAC transcription factor MlNAC12 positively mediates abiotic stress tolerance in transgenic Arabidopsis.

Plant Sci 2018 Dec 25;277:229-241. Epub 2018 Sep 25.

Key Laboratory of Biofuels, Shandong Provincial Key Laboratory of Energy Genetics, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, 266101, China. Electronic address:

NAC (NAM, ATAF1/2 and CUC2) transcription factors play critical roles in plant abiotic stress responses. However, knowledge regarding the functional roles of NACs in abiotic stress tolerance and its underlying mechanisms is relatively limited in Miscanthus. In this study, we functionally characterized a novel Miscanthus NAC gene MlNAC12 by ectopic expression in Arabidopsis. Read More

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December 2018
1 Read

Solar Light Induced Photon-Assisted Synthesis of TiO₂ Supported Highly Dispersed Ru Nanoparticle Catalysts.

Materials (Basel) 2018 Nov 19;11(11). Epub 2018 Nov 19.

Institut de Chimie et Procédés pour l'Energie, l'Environnement et la Santé, CNRS/University of Strasbourg, 25 rue Becquerel, 67087 Strasbourg, France.

Ru/TiO₂ are promising heterogeneous catalysts in different key-reactions taking place in the catalytic conversion of biomass towards fuel additives, biofuels, or biochemicals. TiO₂ supported highly dispersed nanometric-size metallic Ru catalysts were prepared at room temperature via a solar light induced photon-assisted one-step synthesis in liquid phase, far smaller Ru nanoparticles with sharper size distribution being synthesized when compared to the catalysts that were prepared by impregnation with thermal reduction in hydrogen. The underlying strategy is based on the redox photoactivity of the TiO₂ semi-conductor support under solar light for allowing the reduction of metal ions pre-adsorbed at the host surface by photogenerated electrons from the conduction band of the semi-conductor in order to get a fine control in terms of size distribution and dispersion, with no need of chemical reductant, final thermal treatment, or external hydrogen. Read More

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November 2018

Down-Selection and Outdoor Evaluation of Novel, Halotolerant Algal Strains for Winter Cultivation.

Front Plant Sci 2018 29;9:1513. Epub 2018 Oct 29.

National Renewable Energy Laboratory, National Bioenergy Center, Golden, CO, United States.

Algae offer promising feedstocks for the production of renewable fuel and chemical intermediates. However, poor outdoor winter cultivation capacity currently limits deployment potential. In this study, 300 distinct algal strains were screened in saline medium to determine their cultivation suitability during winter conditions in Mesa, Arizona. Read More

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October 2018

Sodium Acetate Responses in and the Ubiquitin Ligase Rsp5.

Front Microbiol 2018 16;9:2495. Epub 2018 Oct 16.

Division of Biological Science, Graduate School of Science and Technology, Nara Institute of Science and Technology, Nara, Japan.

Recent studies have revealed the feasibility of sodium acetate as a potentially novel inhibitor/stressor relevant to the fermentation from neutralized lignocellulosic hydrolysates. This mini-review focuses on the toxicity of sodium acetate, which is composed of both sodium and acetate ions, and on the involved cellular responses that it elicits, particularly via the high-osmolarity glycerol (HOG) pathway, the Rim101 pathway, the P-type ATPase sodium pumps Ena1/2/5, and the ubiquitin ligase Rsp5 with its adaptors. Increased understanding of cellular responses to sodium acetate would improve our understanding of how cells respond not only to different stimuli but also to composite stresses induced by multiple components (e. Read More

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October 2018
4 Reads

Advanced integration of fluid dynamics and photosynthetic reaction kinetics for microalgae culture systems.

BMC Syst Biol 2018 Nov 20;12(Suppl 5):93. Epub 2018 Nov 20.

Czech Technical University in Prague, Faculty of Mechanical Engineering, Technická 4, Prague, 160 00, Czech Republic.

Background: Photosynthetic microalgae have been in the spotlight of biotechnological production (biofuels, lipids, etc), however, current barriers in mass cultivation of microalgae are limiting its successful industrialization. Therefore, a mathematical model integrating both the biological and hydrodynamical parts of the cultivation process may improve our understanding of relevant phenomena, leading to further optimization of the microalgae cultivation.

Results: We introduce a unified multidisciplinary simulation tool for microalgae culture systems, particularly the photobioreactors. Read More

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November 2018
1 Read

Lignin-based polymers and nanomaterials.

Curr Opin Biotechnol 2018 Nov 17;56:112-120. Epub 2018 Nov 17.

Department of Microbiology & Cell Science, IFAS, University of Florida, Gainesville, FL 32610, USA; UF Genetics Institute, University of Florida, Gainesville, FL 32610, USA; Florida Center for Renewable Chemicals and Fuels, University of Florida, Gainesville, FL 32610, USA. Electronic address:

Approximately 50 million tons of lignin are currently produced annually as a by-product of the pulp- and paper industry, and this amount is likely to double in the future with the anticipated production of renewable fuels and chemicals from lignocellulosic biomass, as a sustainable alternative to petroleum. The latter process can be expedited by valorizing lignin, which entails making products from lignin that generate additional revenues for biorefineries so that the production of biofuels becomes more competitive with gasoline. Industrially produced lignin is considered a low-value material that is used as a boiler fuel to generate heat and electricity, and as an ingredient of adhesives, cement, and drilling fluids for underwater oil wells. Read More

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November 2018

Enhanced triacylglycerol production in the diatom by inactivation of a Hotdog-fold thioesterase gene using TALEN-based targeted mutagenesis.

Biotechnol Biofuels 2018 12;11:312. Epub 2018 Nov 12.

1Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, No. 2 Xudong Second Road, Wuhan, 430062 People's Republic of China.

Background: In photosynthetic oleaginous microalgae, acyl-CoA molecules are used as substrates for the biosynthesis of membrane glycerolipids, triacylglycerol (TAG) and other acylated molecules. Acyl-CoA can also be directed to beta-oxidative catabolism. They can be utilized by a number of lipid metabolic enzymes including endogenous thioesterases, which catalyze their hydrolysis to release free fatty acids. Read More

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November 2018
4 Reads

Effects of pH on steam explosion extraction of acetylated galactoglucomannan from Norway spruce.

Biotechnol Biofuels 2018 9;11:311. Epub 2018 Nov 9.

1Faculty of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences, Ås, Norway.

Background: Acetylated galactoglucomannan (AcGGM) is a complex hemicellulose found in softwoods such as Norway spruce (). AcGGM has a large potential as a biorefinery feedstock and source of oligosaccharides for high-value industrial applications. Steam explosion is an effective method for extraction of carbohydrates from plant biomass. Read More

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November 2018

Development of a promising microbial platform for the production of dicarboxylic acids from biorenewable resources.

Biotechnol Biofuels 2018 9;11:310. Epub 2018 Nov 9.

Biotechnology Process Engineering Center, Korean Research Institute of Bioscience and Biotechnology (KRIBB), 30 Yeongudanji-ro, Cheongwon-gu, Cheongju-si, Chungcheongbuk-do 28116 Republic of Korea.

Background: As a sustainable industrial process, the production of dicarboxylic acids (DCAs), used as precursors of polyamides, polyesters, perfumes, plasticizers, lubricants, and adhesives, from vegetable oil has continuously garnered interest. Although the yeast has been used as a host for DCA production, additional strains are continually investigated to meet productivity thresholds and industrial needs. In this regard, the yeast , a potential candidate strain, has been screened. Read More

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November 2018
3 Reads

Enhancing enzymatic saccharification of sugarcane bagasse by combinatorial pretreatment and Tween 80.

Biotechnol Biofuels 2018 9;11:309. Epub 2018 Nov 9.

1College of Forestry and Landscape Architecture, Key Laboratory of Energy Plants Resource and Utilization, Ministry of Agriculture, South China Agricultural University, Guangzhou, 510642 People's Republic of China.

Background: The recalcitrant structure of lignocellulosic biomass made it challenging for their bioconversion into biofuels and biochemicals. Pretreatment was required to deconstruct the intact structure by the removal of hemicellulose/lignin, improving the cellulose accessibility of enzyme. Combinatorial pretreatments with liquid hot water/HSO and ethanol/NaOH of sugarcane bagasse were developed to improve enzymatic hydrolysis under mild conditions. Read More

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November 2018
4 Reads

Draft genome sequence and detailed characterization of biofuel production by oleaginous microalga LWG002611.

Biotechnol Biofuels 2018 9;11:308. Epub 2018 Nov 9.

Bioserve|A CGI Company, 3-1-135/1A, CNR Complex, Mallapur, Hyderabad, Telangana 500 076 India.

Background: Due to scarcity of fossil fuel, the importance of alternative energy sources is ever increasing. The oleaginous microalgae have demonstrated their potential as an alternative source of energy, but have not achieved commercialization owing to some biological and technical inefficiency. Modern methods of recombinant strain development for improved efficacy are suffering due to inadequate knowledge of genome and limited molecular tools available for their manipulation. Read More

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November 2018
3 Reads

Improved xylose tolerance and 2,3-butanediol production of by directed evolution of and the mechanisms revealed by transcriptomics.

Biotechnol Biofuels 2018 9;11:307. Epub 2018 Nov 9.

1Key Laboratory of Industrial Fermentation Microbiology of Ministry of Education, Tianjin Industrial Microbiology Key Lab, College of Biotechnology of Tianjin University of Science and Technology, Tianjin, 300547 China.

Background: The biological production of 2,3-butanediol from xylose-rich raw materials from is a low-cost process. , an encoding gene of the sigma factor, is the key element in global transcription machinery engineering and has been successfully used to improve the fermentation with . However, whether it can regulate the tolerance in remains unclear. Read More

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November 2018
1 Read

Rewiring glycerol metabolism for enhanced production of poly-γ-glutamic acid in .

Biotechnol Biofuels 2018 9;11:306. Epub 2018 Nov 9.

1State Key Laboratory of Biocatalysis and Enzyme Engineering, Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, Environmental Microbial Technology Center of Hubei Province, College of Life Sciences, Hubei University, 368 Youyi Avenue, Wuhan, 430062 Hubei People's Republic of China.

Background: Poly-γ-glutamic acid (γ-PGA) is a natural polymer with great potential applications in areas of agriculture, industry, and pharmaceutical. The biodiesel-derived glycerol can be used as an attractive feedstock for γ-PGA production due to its availability and low price; however, insufficient production of γ-PGA from glycerol is limitation.

Results: The metabolic pathway of WX-02 was rewired to improve the efficiency of glycerol assimilation and the supply of NADPH for γ-PGA synthesis. Read More

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November 2018
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Genome mining of 2-phenylethanol biosynthetic genes from sp. CGMCC 5087 and heterologous overproduction in .

Biotechnol Biofuels 2018 8;11:305. Epub 2018 Nov 8.

1CAS Key Laboratory of Biobased Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, No.189 Songling Road, Laoshan District, Qingdao, 266101 China.

Background: 2-Phenylethanol (2-PE) is a higher aromatic alcohol that is widely used in the perfumery, cosmetics, and food industries and is also a potentially valuable next-generation biofuel. In our previous study, a new strain sp. CGMCC 5087 was isolated to produce 2-PE from glucose through the phenylpyruvate pathway. Read More

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November 2018
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Fractionation and characterization of lignin streams from unique high-lignin content endocarp feedstocks.

Biotechnol Biofuels 2018 8;11:304. Epub 2018 Nov 8.

1Biosystems and Agricultural Engineering, University of Kentucky, Lexington, KY 40506 USA.

Background: Lignin is a promising source of building blocks for upgrading to valuable aromatic chemicals and materials. Endocarp biomass represents a non-edible crop residue in an existing agricultural setting which cannot be used as animal feed nor soil amendment. With significantly higher lignin content and bulk energy density, endocarps have significant advantages to be converted into both biofuel and bioproducts as compared to other biomass resources. Read More

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November 2018
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Cloning, purification, and characterization of an organic solvent-tolerant chitinase, MtCh509, from DAU221.

Biotechnol Biofuels 2018 8;11:303. Epub 2018 Nov 8.

Department of Biotechnology, Dong-A University, Busan, 49315 Republic of Korea.

Background: The ability to use organic solvents in enzyme reactions offers a number of industrially useful advantages. However, most enzymes are almost completely inactive in the presence of organic solvents. Thus, organic solvent-tolerant enzymes have potential applications in industrial processes. Read More

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November 2018
6 Reads

Dye-decolorizing peroxidases in combining the catalytic properties of heme peroxidases and laccase play important roles in ligninolytic system.

Biotechnol Biofuels 2018 8;11:302. Epub 2018 Nov 8.

2Key Laboratory for Feed Biotechnology of the Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, No. 12 South Zhongguancun Street, Beijing, 100081 China.

Background: The white rot fungus exhibits a great potential in biopretreatment of lignocellulose as well as in biodegradation of xenobiotic compounds by extracellular ligninolytic enzymes. Among these enzymes, the possible involvement of dye-decolorizing peroxidase (DyP) in lignin degradation is not clear yet.

Results: Based on the extracellular enzyme activities and secretome analysis, CD2 produced DyPs as the main ligninolytic enzymes when grown in Kirk's medium supplemented with lignin. Read More

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November 2018
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Metagenomic analysis of uncultured microorganisms and their enzymatic attributes.

J Microbiol Methods 2018 Nov 16;155:65-69. Epub 2018 Nov 16.

Department of Biological Sciences, Faculty of Sciences, King Abdulaziz University, Jeddah, Saudi Arabia. Electronic address:

Although second generation biofuel technology is a sustainable route for bioethanol production it is not currently a robust technology because of certain hindrances viz., unavailability of potential enzyme resources, low efficiency of enzymes and restricted availability of potent enzymes that work under harsh conditions in industrial processes. Therefore, bioprospecting of extremophilic microorganisms using metagenomics is a promising alternative to discover novel microbes and enzymes with efficient tolerance to unfavourable conditions and thus could revolutionize the energy sector. Read More

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November 2018
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2.026 Impact Factor

Metal Corrosion and the Efficiency of Corrosion Inhibitors in Less Conductive Media.

J Vis Exp 2018 Nov 3(141). Epub 2018 Nov 3.

Department of Petroleum Technology and Alternative Fuels, University of Chemistry and Technology Prague;

Material corrosion can be a limiting factor for different materials in many applications. Thus, it is necessary to better understand corrosion processes, prevent them and minimize the damages associated with them. One of the most important characteristics of corrosion processes is the corrosion rate. Read More

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November 2018
4 Reads