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    492 results match your criteria Biofabrication[Journal]

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    Proposal to Assess Printability of Bioinks for Extrusion-Based Bioprinting and Evaluation of Rheological Properties Governing Bioprintability.
    Biofabrication 2017 Sep 20. Epub 2017 Sep 20.
    Department for Fnctional Materials in Medicine and Dentistry, Julius-Maximilians-Universität Wurzburg, Wurzburg, GERMANY.
    The development and formulation of printable inks for extrusion-based 3D bioprinting has been a major challenge in the field of biofabrication. Inks, often polymer solutions with the addition of crosslinking to form hydrogels, must not only display adequate mechanical properties for the chosen application, but also show high biocompatibility as well as printability. Here we describe a reproducible two-step method for the assessment of the printability of inks for bioprinting, focussing firstly on screening ink formulations to assess fibre formation and the ability to form 3D constructs before presenting a method for the rheological evaluation of inks to characterise the yield point, shear thinning and recovery behaviour. Read More

    Biomimetic Matrix Fabricated by LMP-1 Gene-Transduced MC3T3-E1 Cells for Bone Regeneration.
    Biofabrication 2017 Sep 20. Epub 2017 Sep 20.
    Guangdong Provincial Key Laboratory of Orthopedics and Traumatology and Department of Spine Surgery, Orthopedic Research Institute, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, CHINA.
    Bone healing is regulated by multiple microenvironmental signals provided by the extracellular matrix (ECM). The study aimed to mimic native osteoinductive microenvironment by developing an ECM using gene-transduced cells. LIM mineralization protein-1 (LMP-1) gene was transferred to the murine pre-osteoblast cells (MC3T3-E1) using lentiviral vectors. Read More

    Double printing of hyaluronic acid / poly(glycidol) hybrid hydrogels with poly(ε-caprolactone) for MSC chondrogenesis.
    Biofabrication 2017 Sep 14. Epub 2017 Sep 14.
    Department for Functional Materials in Medicine and Dentistry, Julius-Maximilians-Universitat Wurzburg, Pleicherwall 2, D17, D-97070 Wurzburg, Wurzburg, GERMANY.
    This study investigates the use of allyl-functionalized poly(glycidol)s (P(AGE-co-G)) as cytocompatible cross-linker for thiol-functionalized hyaluronic acid (HA-SH) and the optimization of this hybrid hydrogel as bioink for 3D bioprinting. Chemical cross-linking of gels with 10 wt.% overall polymer concentration was achieved by UV-induced radical thiol-ene coupling between the thiol and allyl groups. Read More

    A 3D printed microfluidic perfusion device for multicellular spheroid cultures.
    Biofabrication 2017 Sep 11;9(4):045005. Epub 2017 Sep 11.
    Department of Biomedical Engineering, National University of Singapore, 4, Engineering Drive 3, E4-04-10, Singapore 117583, Singapore.
    The advent of 3D printing technologies promises to make microfluidic organ-on-chip technologies more accessible for the biological research community. To date, hydrogel-encapsulated cells have been successfully incorporated into 3D printed microfluidic devices. However, there is currently no 3D printed microfluidic device that can support multicellular spheroid culture, which facilitates extensive cell-cell contacts important for recapitulating many multicellular functional biological structures. Read More

    Laser processing of protein films as a method for accomplishment of cell patterning at the microscale.
    Biofabrication 2017 Sep 7;9(4):045004. Epub 2017 Sep 7.
    Department of Materials Science and Technology, University of Crete, Vassilika Vouton, 710 03 Heraklion, Crete, Greece. Institute of Electronic Structure and Laser (IESL), Foundation for Research and Technology Hellas (FORTH), PO Box 527, Vassilika Vouton, 711 10 Heraklion, Crete, Greece.
    In this study, we propose a photostructuring approach for protein films based on a treatment with nanosecond pulses of a KrF excimer laser. As a model protein we used an amyloid fibril-forming protein. Laser treatment induced a foaming of the sample surface exhibiting an interconnected fibrous mesh with a high degree of control and precision. Read More

    Green Bioprinting: Extrusion-based fabrication of plant cell-laden biopolymer hydrogels scaffolds.
    Biofabrication 2017 Aug 24. Epub 2017 Aug 24.
    Centre for Translational Bone, Joint and Soft Tissue Research, Technische Universitaet Dresden, Fetscherstrasse 74, Dresden, 01307, GERMANY.
    Plant cell cultures produce active agents for pharmaceuticals, food and cosmetics. However, up to now process control for plant cell suspension cultures is challenging. A positive impact of cell immobilization, such as encapsulation in hydrogel beads, on secondary metabolites production has been reported for several plant species. Read More

    Engineering the mechanical and biological properties of nanofibrous vascular grafts for in situ vascular tissue engineering.
    Biofabrication 2017 Aug 17;9(3):035007. Epub 2017 Aug 17.
    Department of Bioengineering, University of California, Berkeley, CA 94720, United States of America. UC Berkeley and UCSF Bioengineering Graduate Program, Berkeley, CA94720, United States of America.
    Synthetic small diameter vascular grafts have a high failure rate, and endothelialization is critical for preventing thrombosis and graft occlusion. A promising approach is in situ tissue engineering, whereby an acellular scaffold is implanted and provides stimulatory cues to guide the in situ remodeling into a functional blood vessel. An ideal scaffold should have sufficient binding sites for biomolecule immobilization and a mechanical property similar to native tissue. Read More

    A microfluidic platform for modeling metastatic cancer cell matrix invasion.
    Biofabrication 2017 Sep 1;9(4):045001. Epub 2017 Sep 1.
    Department of Biomedical Engineering, Boston University, Boston, MA 02215, United States of America.
    Invasion of the extracellular matrix is a critical step in the colonization of metastatic tumors. The invasion process is thought to be driven by both chemokine signaling and interactions between invading cancer cells and physical components of the metastatic niche, including endothelial cells that line capillary walls and serve as a barrier to both diffusion and invasion of the underlying tissue. Transwell chambers, a tool for generating artificial chemokine gradients to induce cell migration, have facilitated recent work to investigate the chemokine contributions to matrix invasion. Read More

    Quantitative criteria to benchmark new and existing bio-inks for cell compatibility.
    Biofabrication 2017 Sep 1;9(4):044102. Epub 2017 Sep 1.
    Recent advancements in 3D bioprinting have led to the fabrication of more complex, more precise, and larger printed tissue constructs. As the field continues to advance, it is critical to develop quantitative benchmarks to compare different bio-inks for key cell-biomaterial interactions, including (1) cell sedimentation within the ink cartridge, (2) cell viability during extrusion, and (3) cell viability after ink curing. Here we develop three simple protocols for quantitative analysis of bio-ink performance. Read More

    GelMA-collagen blends enable drop-on-demand 3D printablility and promote angiogenesis.
    Biofabrication 2017 Sep 1;9(4):045002. Epub 2017 Sep 1.
    Department of Dental Materials and Biomaterials Research, RWTH Aachen University Hospital, Aachen, Germany.
    Effective vascularization is crucial for three-dimensional (3D) printed hydrogel-cell constructs to efficiently supply cells with oxygen and nutrients. Till date, several hydrogel blends have been developed that allow the in vitro formation of a capillary-like network within the gels but comparatively less effort has been made to improve the suitability of the materials for a 3D bioprinting process. Therefore, we hypothesize that tailored hydrogel blends of photo-crosslinkable gelatin and type I collagen exhibit favorable 3D drop-on-demand printing characteristics in terms of rheological and mechanical properties and that further capillary-like network formation can be induced by co-culturing human umbilical vein endothelial cells and human mesenchymal stem cells within the proposed blends. Read More

    Fabrication of arbitrary 3D components in cardiac surgery: from macro-, micro- to nanoscale.
    Biofabrication 2017 Aug 3;9(3):032002. Epub 2017 Aug 3.
    Institute of Biomaterials and Tissue Engineering, Huaqiao University, Xiamen 361021, People's Republic of China. Fujian Provincial Key Laboratory of Biochemical Technology, Xiamen 361021, People's Republic of China.
    Fabrication of tissue-/organ-like structures at arbitrary geometries by mimicking the properties of the complex material offers enormous interest to the research and clinical applicability in cardiovascular diseases. Patient-specific, durable, and realistic three-dimensional (3D) cardiac models for anatomic consideration have been developed for education, pro-surgery planning, and intra-surgery guidance. In cardiac tissue engineering (TE), 3D printing technology is the most convenient and efficient microfabrication method to create biomimetic cardiovascular tissue for the potential in vivo implantation. Read More

    Comparative study of gelatin methacrylate hydrogels from different sources for biofabrication applications.
    Biofabrication 2017 Aug 21;9(4):044101. Epub 2017 Aug 21.
    School of Engineering, Faculty of Applied Sciences, University of British Columbia, Kelowna, BC, V1V 1V7, Canada.
    Gelatin methacrylate (GelMA) hydrogel is a promising bioink for biofabrication applications due to its cost-effectiveness, ease of synthesis and biocompatibility to allow cell adhesion. However, the GelMA synthesized from a widely used porcine skin gelatin has a thermal gelation problem at room temperature. Here, we present thermally stable GelMA hydrogels at room temperature while maintaining the mechanical and biological properties comparable to porcine GelMA. Read More

    Engineering-derived approaches for iPSC preparation, expansion, differentiation and applications.
    Biofabrication 2017 Jul 31;9(3):032001. Epub 2017 Jul 31.
    Department of Mechanical Engineering, 111 'Biomanufacturing and Engineering Living Systems' Innovation International Talents Base, Tsinghua University, Beijing, People's Republic of China.
    Remarkable achievements have been made since induced pluripotent stem cells (iPSCs) were first introduced in 2006. Compared with non-pluripotent stem cells, iPSC research faces several additional complexities, such as the choice of extracellular matrix proteins, growth and differentiation factors, as well as technical challenges related to self-renewal and directed differentiation. Overcoming these challenges requires the integration of knowledge and technologies from multiple fields including cell biology, biomaterial science, engineering, physics and medicine. Read More

    DC biased low-frequency insulating constriction dielectrophoresis for protein biomolecules concentration.
    Biofabrication 2017 Sep 1;9(4):045003. Epub 2017 Sep 1.
    Lane Department of Computer Science and Electrical Engineering, West Virginia University, Morgantown, West Virginia 26506, United States of America.
    Sample enrichment or molecules concentration is considered an essential step in sample processing of miniaturized devices aimed at biosensing and bioanalysis. Among all the means involved to achieve this aim, dielectrophoresis (DEP) is increasingly employed in molecules manipulation and concentration because it is non-destructive and high efficiency. This paper presents a methodology to achieve protein concentration utilizing the combination effects of electrokinetics and low frequency insulating dielectrophoresis (iDEP) generated within a microfluidic device, in which a submicron constricted channel was fabricated using DNA molecular combing and replica molding. Read More

    A novel cylindrical microwell featuring inverted-pyramidal opening for efficient cell spheroid formation without cell loss.
    Biofabrication 2017 Aug 14;9(3):035006. Epub 2017 Aug 14.
    Medical Device Research Center, Research Institute for Future Medicine, Samsung Medical Center, Seoul 06351, Republic of Korea.
    Spheroid cultures have been often used to simulate and understand in situ biological occurrences with potential to be further applied to therapeutic approaches, such as cell transplantation. However, traditional lab-scale techniques hardly reached the needed large scale production of cell spheroids, thus limiting their versatility in many biomedical fields. Microscale technologies have rapidly improved in the last decade, and contributed to the large scale production of cell spheroids with high controllability and reproducibility. Read More

    Towards 4D printed scaffolds for tissue engineering: exploiting 3D shape memory polymers to deliver time-controlled stimulus on cultured cells.
    Biofabrication 2017 Aug 2;9(3):031001. Epub 2017 Aug 2.
    University of Twente, MIRA Institute for Biomedical Technology and Technical Medicine, Department of Tissue Regeneration, 7500AE, Enschede, The Netherlands.
    Tissue engineering needs innovative solutions to better fit the requirements of a minimally invasive approach, providing at the same time instructive cues to cells. The use of shape memory polyurethane has been investigated by producing 4D scaffolds via additive manufacturing technology. Scaffolds with two different pore network configurations (0/90° and 0/45°) were characterized by dynamic-mechanical analysis. Read More

    Rising to the challenge: applying biofabrication approaches for better drug and chemical product development.
    Biofabrication 2017 Jul 19;9(3):033001. Epub 2017 Jul 19.
    National Centre for the Replacement, Refinement and Reduction of Animals in Research, London NW1 2BE, United Kingdom.
    Many industrial sectors, from pharmaceuticals to consumer products, are required to provide data on their products to demonstrate their efficacy and that they are safe for patients, consumers and the environment. This period of testing typically requires the use of animal models, the validity of which has been called into question due to the high rates of attrition across many industries. There is increasing recognition of the limitations of animal models and demands for safety and efficacy testing paradigms which embrace the latest technological advances and knowledge of human biology. Read More

    Mussel-inspired nano-building block assemblies for mimicking extracellular matrix microenvironments with multiple functions.
    Biofabrication 2017 Aug 3;9(3):035005. Epub 2017 Aug 3.
    Key Lab of Advanced Technologies of Materials, Ministry of Education, School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu, Sichuan, 610031, People's Republic of China.
    The assembly of nano-building blocks is an effective way to produce artificial extracellular matrix microenvironments with hierarchical micro/nano structures. However, it is hard to assemble different types of nano-building blocks, to form composite coatings with multiple functions, by traditional layer-by-layer (LbL) self-assembly methods. Inspired by the mussel adhesion mechanism, we developed polydopamine (PDA)-decorated bovine serum albumin microspheres (BSA-MS) and nano-hydroxyapatite (nano-HA), and assembled them to form bioactive coatings with micro/nano structures encapsulating bone morphogenetic protein-2 (BMP-2). Read More

    Bioprinting of a functional vascularized mouse thyroid gland construct.
    Biofabrication 2017 Aug 18;9(3):034105. Epub 2017 Aug 18.
    Laboratory for Biotechnological Research '3D Bioprinting Solutions', Moscow 115409, Russia.
    Bioprinting can be defined as additive biofabrication of three-dimensional (3D) tissues and organ constructs using tissue spheroids, capable of self-assembly, as building blocks. The thyroid gland, a relatively simple endocrine organ, is suitable for testing the proposed bioprinting technology. Here we report the bioprinting of a functional vascularized mouse thyroid gland construct from embryonic tissue spheroids as a proof of concept. Read More

    Three-dimensional (3D) culture in sarcoma research and the clinical significance.
    Biofabrication 2017 Aug 3;9(3):032003. Epub 2017 Aug 3.
    Department of Orthopedics, The Affiliated Tumor Hospital of Zhengzhou University, Zhengzhou, Henan 450008, People's Republic of China. Sarcoma Biology Laboratory, Department of Orthopaedic Surgery, Massachusetts General Hospital and Harvard Medical School, 55 Fruit Street, Jackson 1115, Boston, MA 02114, United States of America.
    Sarcomas are rare malignant tumors that arise from transformed cells of mesenchymal origin. Despite the progress in diagnosis and treatment, sarcomas have a high mortality rate due to local recurrence, metastasis, and the development of drug resistance to chemotherapy. New models for sarcoma research are required to further understand the disease and to develop new therapies. Read More

    Decellularized extracellular matrix: a step towards the next generation source for bioink manufacturing.
    Biofabrication 2017 Aug 4;9(3):034104. Epub 2017 Aug 4.
    Department of Mechanical Engineering, Pohang University of Science and Technology, Republic of Korea.
    In tissue engineering, the need for hierarchical assembly of three-dimensional (3D) tissues has become increasingly important, considering that new technology is essential for advanced tissue fabrication. 3D cell printing has emerged as a powerful technology to recapitulate the microenvironment of native tissue, allowing for the precise deposition of multiple cells onto the pre-defined position. Parallel to these technological advances, the search for an appropriate bioink that can provide a suitable microenvironment supporting cellular activities has been in the spotlight. Read More

    Development of a clay based bioink for 3D cell printing for skeletal application.
    Biofabrication 2017 Jul 25;9(3):034103. Epub 2017 Jul 25.
    Centre for Translational Bone, Joint and Soft Tissue Research, University Hospital Carl Gustav Carus and Faculty of Medicine, Technische Universität Dresden, Dresden, Germany.
    Three-dimensional printing of cell-laden hydrogels has evolved as a promising approach on the route to patient-specific or complex tissue-engineered constructs. However, it is still challenging to print structures with both, high shape fidelity and cell vitality. Herein, we used a synthetic nanosilicate clay, called Laponite, to build up scaffolds utilising the extrusion-based method 3D plotting. Read More

    Correlating rheological properties and printability of collagen bioinks: the effects of riboflavin photocrosslinking and pH.
    Biofabrication 2017 Jul 5;9(3):034102. Epub 2017 Jul 5.
    Meinig School of Biomedical Engineering, Cornell University, Ithaca, NY, United States of America.
    Collagen has shown promise as a bioink for extrusion-based bioprinting, but further development of new collagen bioink formulations is necessary to improve their printability. Screening these formulations by measuring print accuracy is a costly and time consuming process. We hypothesized that rheological properties of the bioink before, during, and/or after gelation can be used to predict printability. Read More

    Cell-instructive high-resolution micropatterned polylactic acid surfaces.
    Biofabrication 2017 Jul 24;9(3):035004. Epub 2017 Jul 24.
    Department of Tissue Regeneration, MIRA Institute for Biomedical Technology and Technical Medicine, University of Twente, Enschede, Overijssel, Netherlands. Department of Instructive Biomaterials Engineering, MERLN Institute for Technology-Inspired Regenerative Medicine, Maastricht University, Maastricht, Limburg, Netherlands.
    Micro and nanoscale topographical structuring of biomaterial surfaces has been a valuable tool for influencing cell behavior, including cell attachment, proliferation and differentiation. However, most fabrication techniques for surface patterning of implantable biomaterials suffer from a limited resolution, not allowing controlled generation of sub-cellular three-dimensional features. Here, a direct laser lithography technique based on two-photon absorption was used to construct several patterns varying in size between 500 nm and 15 μm. Read More

    Birefringence of flow-assembled chitosan membranes in microfluidics.
    Biofabrication 2017 Jun 30;9(3):034101. Epub 2017 Jun 30.
    Department of Mechanical Engineering, Catholic University of America, Washington, DC, 20064, United States of America.
    Biopolymer membrane assembly in microfluidics offers precise spatial and temporal resolution for biomolecular and cellular interactions during and after assembly. Control over molecular transport across the biofabricated membranes requires microstructural characterization. This study investigates, for the first time, the birefringence of chitosan membranes assembled with flow in a microfluidic environment, and the effects of pH and flow rate on the membrane's micro-alignment. Read More

    Scaling-up of a HepaRG progenitor cell based bioartificial liver: optimization for clinical application and transport.
    Biofabrication 2017 Jun 30;9(3):035001. Epub 2017 Jun 30.
    Surgical laboratory, Academic Medical Center, University of Amsterdam, The Netherlands. Tytgat Institute for Liver and Intestinal Research, Academic Medical Centre, University of Amsterdam, The Netherlands.
    A new generation of bioartificial livers, based on differentiated proliferative hepatocyte sources, has been developed. Several practicable and regulatory demands have to be addressed before these can be clinically evaluated. We identified three main hurdles: (1) expansion and preservation of the biocomponent, (2) development of scaled-up culture conditions and (3) transport of the device to the bedside. Read More

    A bioprintable form of chitosan hydrogel for bone tissue engineering.
    Biofabrication 2017 Jul 13;9(3):035003. Epub 2017 Jul 13.
    Hacettepe University, Bioengineering Department 06800, Beytepe, Ankara, Turkey.
    Bioprinting can be defined as 3D patterning of living cells and other biologics by filling and assembling them using a computer-aided layer-by-layer deposition approach to fabricate living tissue and organ analogs for tissue engineering. The presence of cells within the ink to use a 'bio-ink' presents the potential to print 3D structures that can be implanted or printed into damaged/diseased bone tissue to promote highly controlled cell-based regeneration and remineralization of bone. In this study, it was shown for the first time that chitosan solution and its composite with nanostructured bone-like hydroxyapatite (HA) can be mixed with cells and printed successfully. Read More

    Printing-induced cell injury evaluation during laser printing of 3T3 mouse fibroblasts.
    Biofabrication 2017 Jun 20;9(2):025038. Epub 2017 Jun 20.
    School of Naval Architecture and Ocean Engineering, Huazhong University of Science and Technology, Wuhan 430074, People's Republic of China. Dept. of Mechanical and Aerospace Engineering, Univ. of Florida, Gainesville, FL 32611, United States of America.
    Three-dimensional bioprinting has emerged as a promising solution for the freeform fabrication of living cellular constructs, which can be used for tissue/organ transplantation and tissue models. During bioprinting, some living cells are unavoidably injured and may become necrotic or apoptotic cells. This study aims to investigate the printing-induced cell injury and evaluates injury types of post-printing cells using the annexin V/7-aminoactinomycin D and FAM-DEVD-FMK/propidium iodide assays during laser printing of NIH 3T3 mouse fibroblasts. Read More

    3D-printed bioceramic scaffolds with antibacterial and osteogenic activity.
    Biofabrication 2017 Jun 20;9(2):025037. Epub 2017 Jun 20.
    State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai 200050, People's Republic of China. Shanghai Engineering Research Center of Single Crystal Silicon Carbide, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai 200050, People's Republic of China.
    Bacterial infection poses a significant risk with the wide application of bone graft materials. Designing bone grafts with good antibacterial performance and excellent bone-forming activity is of particular significance for bone tissue engineering. In our study, a 3D printing method was used to prepare β-tricalcium phosphate (β-TCP) bioceramic scaffolds. Read More

    Repairing a bone defect with a three-dimensional cellular construct composed of a multi-layered cell sheet on electrospun mesh.
    Biofabrication 2017 Jun 20;9(2):025036. Epub 2017 Jun 20.
    State Key Laboratory of Organic-Inorganic Composites; Beijing Laboratory of Biomedical Materials; Beijing University of Chemical Technology, Beijing 100029, People's Republic of China.
    In addition to providing maneuverability, electrospun nanofibrous meshes can make excellent supports for constructing flexible cell sheets to regulate cell behavior by nanofiber features. With the target of bone regeneration, herein composite nanofibers with two different fiber arrangements (nestlike, random) were electrospun from a blend solution containing poly(l-lactide) (PLLA) and gelatin (1:1 in weight ratio). Unlike the non-woven morphology in a random nanofibrous mesh, PLLA/gelatin composite nanofibers in the nestlike nanofibrous mesh displayed both non-woven and parallel morphologies. Read More

    A heuristic computational model of basic cellular processes and oxygenation during spheroid-dependent biofabrication.
    Biofabrication 2017 Jun 15;9(2):024104. Epub 2017 Jun 15.
    Department of Mechanical Engineering, Indiana University-Purdue University at Indianapolis, IN, United States of America.
    An emerging approach in biofabrication is the creation of 3D tissue constructs through scaffold-free, cell spheroid-only methods. The basic mechanism in this technology is spheroid fusion, which is driven by the minimization of energy, the same biophysical mechanism that governs spheroid formation. However, other factors such as oxygen and metabolite accessibility within spheroids impact on spheroid properties and their ability to form larger-scale structures. Read More

    Synthetic scaffolds with full pore interconnectivity for bone regeneration prepared by supercritical foaming using advanced biofunctional plasticizers.
    Biofabrication 2017 Jun 30;9(3):035002. Epub 2017 Jun 30.
    Institut de Ciència de Materials de Barcelona (CSIC), Campus de la UAB, E-08193 Bellaterra, Spain.
    Supercritical foaming allows for the solvent-free processing of synthetic scaffolds for bone regeneration. However, the control on the pore interconnectivity and throat pore size with this technique still needs to be improved. The use of plasticizers may help overcome these limitations. Read More

    Study of gelatin as an effective energy absorbing layer for laser bioprinting.
    Biofabrication 2017 Jun 9;9(2):024103. Epub 2017 Jun 9.
    Department of Mechanical and Aerospace Engineering, University of Florida, Gainesville, FL 32611, United States of America.
    Laser-induced forward transfer printing, also commonly known as laser printing, has been widely implemented for three-dimensional bioprinting due to its unique orifice-free nature during printing. However, the printing quality has the potential to be further improved for various laser bioprinting applications. The objectives of this study are to investigate the feasibility of using gelatin as an energy absorbing layer (EAL) material for laser bioprinting and its effects on the quality of printed constructs, bioink printability, and post-printing cell viability and process-induced DNA damage. Read More

    Mesenchymal stem cells support growth and organization of host-liver colorectal-tumor organoids and possibly resistance to chemotherapy.
    Biofabrication 2017 Jun 7;9(2):021002. Epub 2017 Jun 7.
    Wake Forest Institute for Regenerative Medicine, Winston-Salem, NC, United States of America. Virginia Tech-Wake Forest University School of Biomedical Engineering and Sciences, Winston-Salem, NC, United States of America.
    Despite having yielded extensive breakthroughs in cancer research, traditional 2D cell cultures have limitations in studying cancer progression and metastasis and screening therapeutic candidates. 3D systems can allow cells to grow, migrate, and interact with each other and the surrounding matrix, resulting in more realistic constructs. Furthermore, interactions between host tissue and developing tumors influence the susceptibility of tumors to drug treatments. Read More

    Current and emerging applications of 3D printing in medicine.
    Biofabrication 2017 Jun 7;9(2):024102. Epub 2017 Jun 7.
    Instructive Biomaterials and Additive Manufacturing Laboratory, Otto H. York Department of Chemical, Biological and Pharmaceutical Engineering, and Department of Bioengineering, New Jersey Institute of Technology, Newark, United States of America.
    Three-dimensional (3D) printing enables the production of anatomically matched and patient-specific devices and constructs with high tunability and complexity. It also allows on-demand fabrication with high productivity in a cost-effective manner. As a result, 3D printing has become a leading manufacturing technique in healthcare and medicine for a wide range of applications including dentistry, tissue engineering and regenerative medicine, engineered tissue models, medical devices, anatomical models and drug formulation. Read More

    Alternately plasma-roughened nanosurface of a hybrid scaffold for aligning myoblasts.
    Biofabrication 2017 Jun 20;9(2):025035. Epub 2017 Jun 20.
    Department of Biomechatronic Engineering, College of Biotechnology and Bioengineering, Sungkyunkwan University, Suwon, Republic of Korea.
    For successful skeletal muscle tissue regeneration, inducing alignment and fusion of myoblasts into multinucleated myotubes is critical. Many studies are ongoing to induce myoblast alignment using various micro/nanopatternings on scaffold surfaces, mechanically stretching scaffolds, or aligned micro/nanofibers. In this study, we have developed a simple method to induce myoblast alignment using a modified plasma treatment on a hybrid PCL scaffold consisting of melt-printed perpendicular PCL struts and an electrospun PCL fibrous mat. Read More

    Cryogenic 3D printing for producing hierarchical porous and rhBMP-2-loaded Ca-P/PLLA nanocomposite scaffolds for bone tissue engineering.
    Biofabrication 2017 Jun 7;9(2):025031. Epub 2017 Jun 7.
    Department of Mechanical Engineering, Faculty of Engineering, The University of Hong Kong, Pokfulam Road, Hong Kong. School of Mechanical Engineering, Dongguan University of Technology, 1 Daxue Road, Songshanhu, Dongguan, Guangdong, People's Republic of China.
    The performance of bone tissue engineering scaffolds can be assessed through cell responses to scaffolds, including cell attachment, infiltration, morphogenesis, proliferation, differentiation, etc, which are determined or heavily influenced by the composition, structure, mechanical properties, and biological properties (e.g. osteoconductivity and osteoinductivity) of scaffolds. Read More

    Direct 3D cell-printing of human skin with functional transwell system.
    Biofabrication 2017 Jun 6;9(2):025034. Epub 2017 Jun 6.
    Department of Mechanical Engineering, Pohang University of Science and Technology (POSTECH), Pohang, Korea.
    Three-dimensional (3D) cell-printing has been emerging as a promising technology with which to build up human skin models by enabling rapid and versatile design. Despite the technological advances, challenges remain in the development of fully functional models that recapitulate complexities in the native tissue. Moreover, although several approaches have been explored for the development of biomimetic human skin models, the present skin models based on multistep fabrication methods using polydimethylsiloxane chips and commercial transwell inserts could be tackled by leveraging 3D cell-printing technology. Read More

    Cell sheet based bioink for 3D bioprinting applications.
    Biofabrication 2017 Jun 26;9(2):024105. Epub 2017 Jun 26.
    Faculty of Engineering and Natural Sciences, Sabanci University, Istanbul, Turkey. 3D Bioprinting Lab, Sabanci University Nanotechnology Research and Application Center, Istanbul, Turkey.
    In this research, a novel development of bioink from cell sheets is presented for scaffold free bioprinting applications. Poly(N-isopropylacrylamide) (PNIPAAm) coated surfaces were first prepared by using initiated chemical vapor deposition method. Cell-sheets were then grown on these thermoresponsive pNIPAAm coated surfaces and easily detached without disturbing delicate cell-extracellular matrix (ECM) and cell-cell interactions. Read More

    3D liver membrane system by co-culturing human hepatocytes, sinusoidal endothelial and stellate cells.
    Biofabrication 2017 May 26;9(2):025022. Epub 2017 May 26.
    Institute on Membrane Technology, National Research Council of Italy, ITM-CNR, c/o University of Calabria, via P. Bucci cubo 17/C, I-87030 Rende (CS), Italy. Department of Chemical Engineering and Materials (DIATIC), University of Calabria, Rende, Italy.
    In this study, a designed approach has been utilized for the development of a 3D liver system. This approach makes use of primary human sinusoidal endothelial cells, stellate cells and hepatocytes that are seeded sequentially on hollow fiber membranes (HF) in order to mimic the layers of cells found in vivo. To this purpose modified polyethersulfone (PES) HF membranes were used for the creation of a 3D human liver system in static and dynamic conditions. Read More

    Fabrication of biomimetic bone grafts with multi-material 3D printing.
    Biofabrication 2017 May 22;9(2):025020. Epub 2017 May 22.
    Department of Biomedical Engineering, Texas A&M University College Station, Texas, 77843-3120, United States of America.
    Extrusion deposition is a versatile method for the 3D printing of biomaterials such as hydrogels, ceramics, and suspensions. Recently, a new class of emulsion inks were developed that can be used to create tunable, hierarchically porous materials with a cure-on-dispense method. Propylene fumarate dimethacrylate (PFDMA) was selected to fabricate bone grafts using this technology due to its established biocompatibility, osteoconductivity, and good compressive properties. Read More

    Design of electrohydrodynamic sprayed polyethylene glycol hydrogel microspheres for cell encapsulation.
    Biofabrication 2017 May 18;9(2):025019. Epub 2017 May 18.
    Department of Biomedical Engineering, Saint Louis University, Saint Louis, MO, United States of America.
    Electrohydrodynamic spraying (EHS) has recently gained popularity for microencapsulation of cells for applications in cell delivery and tissue engineering. Some of the polymers compatible with EHS are alginate, chitosan, and other similar natural polymers, which are subject to ionotropic or physical gelation. It is desirable to further extend the use of the EHS technique beyond such polymers for wider biofabrication applications. Read More

    Microtube array membrane bioreactor promotes neuronal differentiation and orientation.
    Biofabrication 2017 May 17;9(2):025018. Epub 2017 May 17.
    Institute on Membrane Technology, National Research Council of Italy, ITM-CNR, c/o University of Calabria, via P. Bucci cubo 17/C, I-87030 Rende (CS), Italy.
    An important challenge in neuronal tissue engineering is to create innovative tools capable of promoting cellular response in terms of neuronal differentiation and neurite orientation that may be used as investigational platforms for studying neurobiological events and neurodegenerative disorders. A novel membrane bioreactor was created to provide a 3D well-controlled microenvironment for neuronal outgrowth. The bioreactor consisted of poly-L-lactic acid highly aligned microtube array (PLLA-MTA) membranes assembled in parallel within a chamber that establish an intraluminal and an extraluminal compartment whose communication occurs through the pores of the MTA membrane walls. Read More

    Simultaneous microfluidic spinning of multiple strands of submicron fiber for the production of free-standing porous membranes for biological application.
    Biofabrication 2017 Jun 1;9(2):025026. Epub 2017 Jun 1.
    KU-KIST Graduate School of Converging Science and Technology, Korea University, 145 Anam-ro, Seongbuk-gu, Seoul, 02841, Republic of Korea.
    Microfibers produced using electrospinning and microfluidics-based technologies have been developed as a powerful tool in tissue engineering applications such as drug delivery and scaffolds. The applications of these fibers, however, have been limited because of the hazardous solvents used to make them, difficulties in controlling the pore sizes of their membrane forms, and downscaling the size of the fiber. Nevertheless, extending the use of these fibers, for example in the production of a free-standing porous membrane appropriate for cell-based research, is highly needed for tissue engineering, organ-on-a-chip, and drug delivery research and applications. Read More

    Importance of endogenous extracellular matrix in biomechanical properties of human skin model.
    Biofabrication 2017 May 11;9(2):025017. Epub 2017 May 11.
    Institut de Pharmacologie et de Biologie Structurale, Université de Toulouse, CNRS, UPS, Toulouse, France.
    The physical and mechanical properties of cells modulate their behavior such proliferation rate, migration and extracellular matrix remodeling. In order to study cell behavior in a tissue-like environment in vitro, it is of utmost importance to develop biologically and physically relevant 3D cell models. Here, we characterized the physical properties of a single cell type growing in configurations of increasing complexity. Read More

    Large scale production and controlled deposition of single HUVEC spheroids for bioprinting applications.
    Biofabrication 2017 Jun 1;9(2):025027. Epub 2017 Jun 1.
    Laboratory for MEMS Applications, IMTEK-Department of Microsystems Engineering, University of Freiburg, Georges-Koehler-Allee 103, D-79110 Freiburg, Germany. Hahn-Schickard, Georges-Koehler-Allee 103, D-79110 Freiburg, Germany.
    We present (1) a fast and automated method for large scale production of HUVEC spheroids based on the hanging drop method and (2) a novel method for well-controlled lateral deposition of single spheroids by drop-on-demand printing. Large scale spheroid production is achieved via printing 1536 droplets of HUVEC cell suspension having a volume of 1 μl each within 3 min at a pitch of 2.3 mm within an array of 48 × 32 droplets onto a flat substrate. Read More

    Leaf-inspired microcontact printing vascular patterns.
    Biofabrication 2017 Jun 1;9(2):021001. Epub 2017 Jun 1.
    School of Engineering, University of California, Merced, United States of America. Graduate Program in Biological Engineering and Small-scale Technologies, University of California, Merced, United States of America.
    The vascularization of tissue grafts is critical for maintaining viability of the cells within a transplanted graft. A number of strategies are currently being investigated including very promising microfluidics systems. Here, we explored the potential for generating a vasculature-patterned endothelial cells that could be integrated into distinct layers between sheets of primary cells. Read More

    Synthesis of cell composite alginate microfibers by microfluidics with the application potential of small diameter vascular grafts.
    Biofabrication 2017 Jun 7;9(2):025030. Epub 2017 Jun 7.
    Key Laboratory for Biorheological Science and Technology of Ministry of Education; State and Local Joint Engineering Laboratory for Vascular Implants; Bioengineering College, Chongqing University, Chongqing, 400044, People's Republic of China.
    Fabrication of small diameter vascular grafts (SDVGs) with appropriate responses for clinical application is still challenging. In the present work, the production and characterization of solid alginate based microfibers as potential SDVG candidates through the method of microfluidics were considered original. A simple glass microfluidic device with a 'L-shape' cylindrical-flow channel in the microfluidic platform was developed. Read More

    Cell patterning via laser micro/nano structured silicon surfaces.
    Biofabrication 2017 May 31;9(2):025024. Epub 2017 May 31.
    Institute of Electronic Structure and Laser, Foundation for Research and Technology-Hellas, Heraklion, 71110, Crete, Greece. Department of Physics, University of Crete, Heraklion, 71003, Crete, Greece.
    The surface topography of biomaterials can have an important impact on cellular adhesion, growth and proliferation. Apart from the overall roughness, the detailed morphological features, at all length scales, significantly affect the cell-biomaterial interactions in a plethora of applications including structural implants, tissue engineering scaffolds and biosensors. In this study, we present a simple, one-step direct laser patterning technique to fabricate nanoripples and dual-rough hierarchical micro/nano structures to control SW10 cell attachment and migration. Read More

    Developing organ-on-a-chip concepts using bio-mechatronic design methodology.
    Biofabrication 2017 May 26;9(2):025023. Epub 2017 May 26.
    Division of Biotechnology, IFM, Linköping University, SE-581 83 Linköping, Sweden.
    Mechatronic design is an engineering methodology for conceiving, configuring and optimising the design of a technical device or product to the needs and requirements of the final user. In this article, we show how the basic principles of this methodology can be exploited for in vitro cell cultures-often referred to as organ-on-a-chip devices. Due to the key role of the biological cells, we have introduced the term bio-mechatronic design, to highlight the complexity of designing a system that should integrate biology, mechanics and electronics in the same device structure. Read More

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