567 results match your criteria Biofabrication[Journal]


Double-layer perfusable collagen microtube device for heterogeneous cell culture.

Biofabrication 2018 Nov 30;11(1):015010. Epub 2018 Nov 30.

Department of Mechanical Engineering, Faculty of Science and Technology, Keio University, Japan.

In vitro perfusable 3D tissue models mimic in vivo tissues and have several benefits in drug testing. However, processes used to fabricate these models often tend to be complicated. Here, we present a double-layer perfusable collagen tube device for multilayered in vitro 3D cell culture. Read More

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November 2018

Porous tissue strands: avascular building blocks for scalable tissue fabrication.

Biofabrication 2018 Nov 23;11(1):015009. Epub 2018 Nov 23.

Engineering Science and Mechanics Department, The Pennsylvania State University, State College, PA, United States of America. The Huck Institutes of the Life Sciences, The Pennsylvania State University, State College, PA, United States of America.

The scalability of cell aggregates such as spheroids, strands, and rings has been restricted by diffusion of nutrient and oxygen into their core. In this study, we introduce a novel concept in generating tissue building blocks with micropores, which represents an alternative solution for vascularization. Sodium alginate porogens were mixed with human adipose-derived stem cells, and loaded into tubular alginate capsules, followed by de-crosslinking of the capsules. Read More

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November 2018
1 Read

Special issue on bioinks.

Biofabrication 2018 Nov 23;11(1):010201. Epub 2018 Nov 23.

Department of Functional Materials in Medicine and Dentistry and Bavarian Polymer Institute, University of Würzburg, Pleicherwall 2, D-97070 Würzburg, Germany.

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November 2018

A definition of bioinks and their distinction from biomaterial inks.

Biofabrication 2018 Nov 23;11(1):013001. Epub 2018 Nov 23.

Department of Functional Materials in Medicine and Dentistry and Bavarian Polymer Institute, University of Würzburg, D-97070 Würzburg, Germany.

Biofabrication aims to fabricate biologically functional products through bioprinting or bioassembly (Groll et al 2016 Biofabrication 8 013001). In biofabrication processes, cells are positioned at defined coordinates in three-dimensional space using automated and computer controlled techniques (Moroni et al 2018 Trends Biotechnol. 36 384-402), usually with the aid of biomaterials that are either (i) directly processed with the cells as suspensions/dispersions, (ii) deposited simultaneously in a separate printing process, or (iii) used as a transient support material. Read More

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November 2018
3 Reads

Perfusable and stretchable 3D culture system for skin-equivalent.

Biofabrication 2018 Nov 15;11(1):011001. Epub 2018 Nov 15.

Center for International Research on Integrative Biomedical Systems, Institute of Industrial Science, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo, 153-8505, Japan. Biotechnology Research Institute for Drug Discovery, National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Higashi, Tsukuba, Ibaraki, 305-8565, Japan.

This study describes a perfusable and stretchable culture system for a skin-equivalent. The system is comprised of a flexible culture device equipped with connections that fix vascular channels of the skin-equivalent and functions as an interface for an external pump. Furthermore, a stretching apparatus for the culture device can be fabricated using rapid prototyping technologies, which allows for easy modifications of stretching parameters. Read More

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November 2018

Reliable autapse formation using the single-cell patterning method.

Biofabrication 2018 Nov 13;11(1):015008. Epub 2018 Nov 13.

Division of WCU (World Class University) Multiscale Mechanical Design, School of Mechanical and Aerospace Engineering, Seoul National University, Seoul, Republic of Korea.

Auto neuronal synapses, or autapses, are aberrant structures where the synaptic contact of a neuron forms onto its own branch. The functions of autapses, however, remain unknown. Here, we introduce a simple patterning method for capturing a single-cell, in which we maintained the isolated cell until it reached maturity, and developed arrays of autapses for electrophysiological analysis using multi-electrode arrays (MEA). Read More

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November 2018

Electrochemical fabrication of a biomimetic elastin-containing bi-layered scaffold for vascular tissue engineering.

Biofabrication 2018 Nov 9;11(1):015007. Epub 2018 Nov 9.

Department of Chemical Engineering, Florida Institute of Technology, Melbourne, FL 32901, United States of America.

Biomimetic tissue-engineered vascular grafts (TEVGs) have immense potential to replace diseased small-diameter arteries (<4 mm) for the treatment of cardiovascular diseases. However, biomimetic approaches developed thus far only partially recapitulate the physicochemical properties of the native vessel. While it is feasible to fabricate scaffolds that are compositionally similar to native vessels (collagen and insoluble elastic matrix) using freeze-drying, these scaffolds do not mimic the aligned topography of collagen and elastic fibers found in native vessels. Read More

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November 2018
1 Read

StarPEG/heparin-hydrogel based in vivo engineering of stable bizonal cartilage with a calcified bottom layer.

Biofabrication 2018 Oct 30;11(1):015001. Epub 2018 Oct 30.

Research Centre for Experimental Orthopaedics, Heidelberg University Hospital, Heidelberg, Germany.

Repaired cartilage tissue lacks the typical zonal structure of healthy native cartilage needed for appropriate function. Current grafts for treatment of full thickness cartilage defects focus primarily on a nonzonal design and this may be a reason why inferior nonzonal regeneration tissue developed in vivo. No biomaterial-based solutions have been developed so far to induce a proper zonal architecture into a non-mineralized and a calcified cartilage layer. Read More

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October 2018
3 Reads

Co-axial wet-spinning in 3D bioprinting: state of the art and future perspective of microfluidic integration.

Biofabrication 2018 Nov 9;11(1):012001. Epub 2018 Nov 9.

Department of Chemistry, University of Rome 'La Sapienza', 00185 Rome, Italy. Warsaw University of Technology, Faculty of Materials Science and Engineering, Warsaw, Poland. Institute of Physical Chemistry, Polish Academy of Sciences, 01224 Warsaw, Poland.

Nowadays, 3D bioprinting technologies are rapidly emerging in the field of tissue engineering and regenerative medicine as effective tools enabling the fabrication of advanced tissue constructs that can recapitulate in vitro organ/tissue functions. Selecting the best strategy for bioink deposition is often challenging and time consuming process, as bioink properties-in the first instance, rheological and gelation-strongly influence the suitable paradigms for its deposition. In this short review, we critically discuss one of the available approaches used for bioprinting-namely co-axial wet-spinning extrusion. Read More

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November 2018
5 Reads

Engineering of microscale vascularized fat that responds to perfusion with lipoactive hormones.

Biofabrication 2018 Oct 30;11(1):014101. Epub 2018 Oct 30.

Department of Biomedical Engineering, Boston University, Boston, MA 02215, United States of America.

Current methods to treat large soft-tissue defects mainly rely on autologous transfer of adipocutaneous flaps, a method that is often limited by donor site availability. Engineered vascularized adipose tissues can potentially be a viable and readily accessible substitute to autologous flaps. In this study, we engineered a small-scale adipose tissue with pre-patterned vasculature that enables immediate perfusion. Read More

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October 2018
5 Reads

Development of a functional airway-on-a-chip by 3D cell printing.

Biofabrication 2018 Oct 30;11(1):015002. Epub 2018 Oct 30.

Department of Mechanical Engineering, Pohang University of Science and Technology (POSTECH), 77 Cheongam-ro, Nam-gu, Pohang, Kyungbuk, 37673, Republic of Korea.

We used 3D cell printing to emulate an airway coupled with a naturally-derived blood vessel network in vitro. Decellularized extracellular matrix bioink derived from porcine tracheal mucosa (tmdECM) was used to encapsulate and print endothelial cells and fibroblasts within a designated polycarprolactone (PCL) frame. Providing a niche that emulates conditions in vivo, tmdECM gradually drives endothelial re-orientation, which leads to the formation of a lumen and blood vessel network. Read More

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October 2018
1 Read

Optimization of collagen type I-hyaluronan hybrid bioink for 3D bioprinted liver microenvironments.

Biofabrication 2018 Oct 30;11(1):015003. Epub 2018 Oct 30.

Wake Forest Institute for Regenerative Medicine, Wake Forest School of Medicine, 391 Technology Way, Winston-Salem, NC, 27101, United States of America. Virginia Tech-Wake Forest School of Biomedical Engineering and Sciences, Wake Forest School of Medicine, Medical Center Boulevard, Winston-Salem, NC, 27157, United States of America.

Current 3D printing of tissue is restricted by the use of biomaterials that do not recapitulate the native properties of the extracellular matrix (ECM). These restrictions have thus far prevented optimization of composition and structure of the in vivo tissue microenvironment. The artificial nature of currently used biomaterials affects cellular phenotype and function of the bioprinted tissues, and results in inaccurate modeling of disease and drug metabolism significantly. Read More

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October 2018
6 Reads

Fabrication of a thick three-dimensional scaffold with an open cellular-like structure using airbrushing and thermal cross-linking of molded short nanofibers.

Biofabrication 2018 Nov 1;11(1):015006. Epub 2018 Nov 1.

School of Dentistry, The University of Queensland, Oral Health Centre, 288 Herston Rd, QLD 4006, Australia. Department of Engineering Materials and Mechanical Design, Faculty of Engineering, South Valley University, Qena 83523, Egypt.

Nanoscale fibers mimicking the extracellular matrix of natural tissue can be produced by conventional electrospinning, but this approach results in two-dimensional thin dense fibrous mats which can hinder effective cell infiltration. The aim of the present study was to design a thick, three-dimensional (3D) cylindrical scaffold with an open pore structure assembled from short polycaprolactone (PCL) fibers using a facile airbrushing approach. In addition, magnesium particles were incorporated into the PCL solution to both enhance the mechanical properties of the scaffold and stimulate cellular activity following cell seeding. Read More

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November 2018
2 Reads

A novel tissue-engineered 3D tumor model for anti-cancer drug discovery.

Biofabrication 2018 Oct 30;11(1):015004. Epub 2018 Oct 30.

State Key Laboratory of Fine Chemicals, Dalian R&D Center for Stem Cell and Tissue Engineering, Dalian University of Technology, Dalian 116024, People's Republic of China.

Cancer biology and drug discovery are heavily dependent on conventional 2D cell culture systems. However, a 2D culture is significantly limited by its ability to reflect 'true biology' of tumor in vivo. Three-dimensional (3D) in vitro cell culture models have been introduced to aid cancer drug discovery by better modeling the tumor microenvironment. Read More

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October 2018
3 Reads

Substrate elasticity dependent colony formation and cardiac differentiation of human induced pluripotent stem cells.

Biofabrication 2018 Oct 30;11(1):015005. Epub 2018 Oct 30.

École Normale Supérieure-PSL Research University, Département de Chimie, Sorbonne Universités-UPMC Univ Paris 06, CNRS UMR 8640 PASTEUR, 24, rue Lhomond, F-75005 Paris, France.

Substrate elasticity regulates cell functions including cell aggregation and stem cell differentiation. The ability to manufacture substrates of desired elasticity over a broad range is therefore crucial for both fundamental research and advanced applications. In this work, we developed a method to fabricate dense elastomer pillars of different heights on a rigid substrate, providing an effective elasticity ranging from 3 to 168 kPa. Read More

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October 2018
1 Read
4.290 Impact Factor

3D bioprinting of a hyaluronan bioink through enzymatic-and visible light-crosslinking.

Biofabrication 2018 09 25;10(4):044104. Epub 2018 Sep 25.

AO Research Institute Davos, Davos Platz, Switzerland. Department of Biomaterials Science and Technology, University of Twente, Enschede, The Netherlands.

Extrusion-based three-dimensional bioprinting relies on bioinks engineered to combine viscoelastic properties for extrusion and shape retention, and biological properties for cytocompatibility and tissue regeneration. To satisfy these conflicting requirements, bioinks often utilize either complex mixtures or complex modifications of biopolymers. In this paper we introduce and characterize a bioink exploiting a dual crosslinking mechanism, where an enzymatic reaction forms a soft gel suitable for cell encapsulation and extrusion, while a visible light photo-crosslinking allows shape retention of the printed construct. Read More

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September 2018
1 Read

Bottom-up biofabrication using microfluidic techniques.

Biofabrication 2018 09 17;10(4):044103. Epub 2018 Sep 17.

Institute of Industrial Science, The University of Tokyo, Tokyo, Japan.

Nature builds living organisms in a bottom-up fashion, starting from the expression of genetic information on a cellular level, to the proliferation, differentiation, and self-assembly of cells into tissues/organs during embryo development and wound-healing processes. To mimic this bottom-up approach, it is essential to handle and manipulate small-scale biomaterials using specific technologies, such as microfluidic techniques. Microfluidics provides the tool-sets that deal with the behavior, precise control and manipulation of small amounts of fluids. Read More

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September 2018
2 Reads

Peroxidase-catalyzed microextrusion bioprinting of cell-laden hydrogel constructs in vaporized ppm-level hydrogen peroxide.

Biofabrication 2018 09 5;10(4):045007. Epub 2018 Sep 5.

Department of Materials Science and Engineering, Graduate School of Engineering Science, Osaka University, Osaka 560-8531, Japan.

Hydrogels were prepared by contacting air containing 10-50 ppm HO with an aqueous solution containing polymer(s) possessing phenolic hydroxyl (Ph) moieties (polymer-Ph) and horseradish peroxidase (HRP). In this system, HRP catalyzes cross-linking of the Ph moieties by consuming HO diffused from the air. The hydrogelation rate and mechanical properties of the resultant hydrogels can be tuned by controlling the HO concentration in air, the exposure time of the air containing HO to the solution containing polymer-Phs and HRP, and the HRP concentration. Read More

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September 2018
3 Reads

TGF-β induced epithelial-mesenchymal transition in an advanced cervical tumor model by 3D printing.

Biofabrication 2018 09 10;10(4):044102. Epub 2018 Sep 10.

Biomanufacturing Center, Dept. of Mechanical Engineering, Tsinghua University, Haidian District, Beijing 100084, People's Republic of China. Biomanufacturing and Rapid Forming Technology Key Laboratory of Beijing, Beijing 100084, People's Republic of China. Overseas Expertise Introduction Center for Discipline Innovation, Tsinghua University, Haidian District, Beijing 100084, People's Republic of China.

An advanced in vitro cervical tumor model was established by 3D printing to study the epithelial-to-mesenchymal transition (EMT), which is a very important stage of dissemination of carcinoma leading to metastatic tumors. A HeLa/hydrogel grid construct composed of gelatin, alginate, Matrigel and HeLa cells was fabricated by forced extrusion in a layer-by-layer fashion. HeLa cells rapidly proliferated, formed spheroids and presented tumorigenic characteristic in the 3D-printed structure. Read More

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September 2018
2 Reads

Pneumatospinning of collagen microfibers from benign solvents.

Biofabrication 2018 08 14;10(4):045004. Epub 2018 Aug 14.

Embody, Norfolk, VA, United States of America. Eastern Virginia Medical School, Norfolk, VA, United States of America.

Introduction: Current collagen fiber manufacturing methods for biomedical applications, such as electrospinning and extrusion, have had limited success in clinical translation, partially due to scalability, cost, and complexity challenges. Here we explore an alternative, simplified and scalable collagen fiber formation method, termed 'pneumatospinning,' to generate submicron collagen fibers from benign solvents.

Methods And Results: Clinical grade type I atelocollagen from calf corium was electrospun or pneumatospun as sheets of aligned and isotropic fibrous scaffolds. Read More

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August 2018
8 Reads

Biofabrication of human articular cartilage: a path towards the development of a clinical treatment.

Biofabrication 2018 08 21;10(4):045006. Epub 2018 Aug 21.

Department of Surgery, St Vincent's Hospital, University of Melbourne, Clinical Sciences Building, 29 Regent Street, 3065 Fitzroy, VIC, Australia. ARC Centre of Excellence for Electromaterials Science, Intelligent Polymer Research Institute, Innovation Campus, University of Wollongong, NSW, Australia. BioFab3D, Aikenhead Centre for Medical Discovery, St Vincent's Hospital, Melbourne, Australia.

Cartilage injuries cause pain and loss of function, and if severe may result in osteoarthritis (OA). 3D bioprinting is now a tangible option for the delivery of bioscaffolds capable of regenerating the deficient cartilage tissue. Our team has developed a handheld device, the Biopen, to allow in situ additive manufacturing during surgery. Read More

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August 2018
1 Read

Fabrication of omega-shaped microwell arrays for a spheroid culture platform using pins of a commercial CPU to minimize cell loss and crosstalk.

Biofabrication 2018 08 14;10(4):045003. Epub 2018 Aug 14.

School of Mechanical Engineering, College of Engineering, Chung-Ang University, 84 Heukseok-ro, Dongjak-gu, Seoul 06974, Republic of Korea.

A cell spheroid culture has the benefit of simulating in vivo three-dimensional cell environments. Microwell systems have been developed to mass-produce large quantities of uniform spheroids, and are frequently used in research areas, such as cell biology, anticancer drug development, and regenerative therapy. Recently reported concave-bottomed microwell systems have delivered more benefits in producing spheroids of higher quality and facilitating more effective research. Read More

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August 2018
2 Reads

Soft-molecular imprinted electrospun scaffolds to mimic specific biological tissues.

Biofabrication 2018 08 20;10(4):045005. Epub 2018 Aug 20.

Research Center E. Piaggio and Department of Ingegneria dell'Informazione, University of Pisa, Pisa, Italy. Department of Tissue Regeneration, MIRA Institute for Biomedical Technology and Technical Medicine, Faculty of Science and Technology, University of Twente, Enschede, The Netherlands.

The fabrication of bioactive scaffolds able to mimic the in vivo cellular microenvironment is a challenge for regenerative medicine. The creation of sites for the selective binding of specific endogenous proteins represents an attractive strategy to fabricate scaffolds able to elicit specific cell response. Here, electrospinning (ESP) and soft-molecular imprinting (soft-MI) techniques were combined to fabricate a soft-molecular imprinted electrospun bioactive scaffold (SMIES) for tissue regeneration. Read More

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August 2018
4 Reads

Bioprinting of mineralized constructs utilizing multichannel plotting of a self-setting calcium phosphate cement and a cell-laden bioink.

Biofabrication 2018 07 27;10(4):045002. Epub 2018 Jul 27.

Centre for Translational Bone, Joint and Soft Tissue Research, Faculty of Medicine Carl Gustav Carus, Technische Universität Dresden, Dresden, Germany.

Due to their characteristic resemblance of the mineral component of bone, calcium phosphates are widely accepted as optimal bone substitute materials. Recent research focused on the development of pasty calcium phosphate cement (CPC) formulations, which can be fabricated into various shapes by low-temperature extrusion-based additive manufacturing, namely 3D plotting. While it could be demonstrated that sensitive substances like growth factors can be integrated in such printed CPC scaffolds without impairment of their biological activity live cells cannot be suspended in CPC as they may not be functional when enclosed in a solid and stiff matrix. Read More

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July 2018
4 Reads

Decellularized corneal lenticule embedded compressed collagen: toward a suturable collagenous construct for limbal reconstruction.

Biofabrication 2018 07 23;10(4):045001. Epub 2018 Jul 23.

Department of Mechanical Engineering, Pohang University of Science and Technology (POSTECH), 77 Cheongam-ro, Pohang, Gyeongbuk, 37673, Republic of Korea.

Recently, compressed collagen has attracted much attention as a potential alternative for a limbal epithelial stem cell (LESC) carrier to treat limbal stem cell deficiency (LSCD), in that it can provide mechanically improved collagen fibrillar structures compared to conventional collagen hydrogel. However, its clinical efficacy as an LESC carrier has not yet been studied through in vivo transplantation due to limited mechanical strength that cannot withstand a force induced by surgical suturing and low resistance to enzymatic degradation. This study firstly presents a suturable LESC carrier based on compressed collagen in the form of a biocomposite. Read More

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July 2018
3 Reads

3D printing human induced pluripotent stem cells with novel hydroxypropyl chitin bioink: scalable expansion and uniform aggregation.

Biofabrication 2018 07 12;10(4):044101. Epub 2018 Jul 12.

Biomanufacturing and Rapid Forming Technology Key Laboratory of Beijing, Department of Mechanical Engineering, Tsinghua University, Beijing, People's Republic of China. 111 'Biomanufacturing and Engineering Living Systems' Innovation International Talents Base, Beijing, People's Republic of China.

Human induced pluripotent stem cells (hiPSCs) are more likely to successfully avoid the immunological rejection and ethical problems that are often encountered by human embryonic stem cells in various stem cell studies and applications. To transfer hiPSCs from the laboratory to clinical applications, researchers must obtain sufficient cell numbers. In this study, 3D cell printing was used as a novel method for iPSC scalable expansion. Read More

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July 2018
3 Reads
4.290 Impact Factor

Optimization of gelatin-alginate composite bioink printability using rheological parameters: a systematic approach.

Biofabrication 2018 06 29;10(3):034106. Epub 2018 Jun 29.

Wake Forest Institute for Regenerative Medicine, Wake Forest School of Medicine, Winston-Salem, North Carolina, United States of America.

Three-dimensional bioprinting has emerged as a promising technique in tissue engineering applications through the precise deposition of cells and biomaterials in a layer-by-layer fashion. However, the limited availability of hydrogel bioinks is frequently cited as a major issue for the advancement of cell-based extrusion bioprinting technologies. It is well known that highly viscous materials maintain their structure better, but also have decreased cell viability due to the higher forces which are required for extrusion. Read More

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June 2018
25 Reads

3D bioprinting of scaffolds with living Schwann cells for potential nerve tissue engineering applications.

Biofabrication 2018 06 29;10(3):035014. Epub 2018 Jun 29.

Department of Mechanical Engineering, College of Engineering, University of Saskatchewan, S7N 5A9, SK, Canada.

Three-dimensional bioprinting of biomaterials shows great potential for producing cell-encapsulated scaffolds to repair nerves after injury or disease. For this, preparation of biomaterials and bioprinting itself are critical to create scaffolds with both biological and mechanical properties appropriate for nerve regeneration, yet remain unachievable. This paper presents our study on bioprinting Schwann cell-encapsulated scaffolds using composite hydrogels of alginate, fibrin, hyaluronic acid, and/or RGD peptide, for nerve tissue engineering applications. Read More

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June 2018
34 Reads

Label free process monitoring of 3D bioprinted engineered constructs via dielectric impedance spectroscopy.

Biofabrication 2018 06 28;10(3):035012. Epub 2018 Jun 28.

Edward P Fitts Department of Industrial and Systems Engineering, North Carolina State University, Raleigh, NC 27695, United States of America. Comparative Medicine Institute, North Carolina State University, Raleigh, NC 27695, United States of America.

Biofabrication processes can affect biological quality attributes of encapsulated cells within constructs. Currently, assessment of the fabricated constructs is performed offline by subjecting the constructs to destructive assays that require staining and sectioning. This drawback limits the translation of biofabrication processes to industrial practice. Read More

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June 2018
7 Reads

Influence of key processing parameters and seeding density effects of microencapsulated chondrocytes fabricated using electrohydrodynamic spraying.

Biofabrication 2018 06 25;10(3):035011. Epub 2018 Jun 25.

Trinity Centre for Bioengineering, Trinity Biomedical Sciences Institute, Trinity College Dublin, The University of Dublin, Dublin, Ireland. School of Engineering, Trinity College Dublin, The University of Dublin, Dublin, Ireland.

Cell delivery and leakage during injection remains a challenge for cell-based intervertebral disc regeneration strategies. Cellular microencapsulation may offer a promising approach to overcome these limitations by providing a protective niche during intradiscal injection. Electrohydrodynamic spraying (EHDS) is a versatile one-step approach for microencapsulation of cells using a high voltage electric field. Read More

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June 2018
7 Reads

Perfusion directed 3D mineral formation within cell-laden hydrogels.

Biofabrication 2018 06 28;10(3):035013. Epub 2018 Jun 28.

Department of Biomedical and Chemical Engineering, Syracuse University, Syracuse, NY, United States of America.

Despite the promise of stem cell engineering and the new advances in bioprinting technologies, one of the major challenges in the manufacturing of large scale bone tissue scaffolds is the inability to perfuse nutrients throughout thick constructs. Here, we report a scalable method to create thick, perfusable bone constructs using a combination of cell-laden hydrogels and a 3D printed sacrificial polymer. Osteoblast-like Saos-2 cells were encapsulated within a gelatin methacrylate (GelMA) hydrogel and 3D printed polyvinyl alcohol pipes were used to create perfusable channels. Read More

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June 2018
6 Reads

Tailoring nanostructure and bioactivity of 3D-printable hydrogels with self-assemble peptides amphiphile (PA) for promoting bile duct formation.

Biofabrication 2018 06 18;10(3):035010. Epub 2018 Jun 18.

Department of Biomedical Engineering Northwestern University, United States of America.

3D-printing has expanded our ability to produce reproducible and more complex scaffold architectures for tissue engineering applications. In order to enhance the biological response within these 3D-printed scaffolds incorporating nanostructural features and/or specific biological signaling may be an effective means to optimize tissue regeneration. Peptides amphiphiles (PAs) are a versatile supramolecular biomaterial with tailorable nanostructural and biochemical features. Read More

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June 2018
10 Reads

Scaffold-free, label-free and nozzle-free biofabrication technology using magnetic levitational assembly.

Biofabrication 2018 06 18;10(3):034104. Epub 2018 Jun 18.

Laboratory for Biotechnological Research '3D Bioprinting Solutions', Moscow, Russia.

Tissue spheroids have been proposed as building blocks in 3D biofabrication. Conventional magnetic force-driven 2D patterning of tissue spheroids requires prior cell labeling by magnetic nanoparticles, meanwhile a label-free approach for 3D magnetic levitational assembly has been introduced. Here we present first time report on rapid assembly of 3D tissue construct using scaffold-free, nozzle-free and label-free magnetic levitation of tissue spheroids. Read More

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June 2018
23 Reads

Bioengineered bile ducts recapitulate key cholangiocyte functions.

Biofabrication 2018 06 12;10(3):034103. Epub 2018 Jun 12.

Department of Clinical Sciences of Companion Animals, Faculty of Veterinary Medicine, Utrecht University, The Netherlands. Hubrecht Institute-KNAW and University Medical Center Utrecht, Utrecht, The Netherlands.

Investigation of diseases of the bile duct system and identification of potential therapeutic targets are hampered by the lack of tractable in vitro systems to model cholangiocyte biology. Here, we show a step-wise method for the differentiation of murine Lgr5 liver stem cells (organoids) into cholangiocyte-like cells (CLCs) using a combination of growth factors and extracellular matrix components. Organoid-derived CLCs display key properties of primary cholangiocytes, such as expressing cholangiocyte markers, forming primary cilia, transporting small molecules and responding to farnesoid X receptor agonist. Read More

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June 2018
17 Reads

Simulations of 3D bioprinting: predicting bioprintability of nanofibrillar inks.

Biofabrication 2018 06 18;10(3):034105. Epub 2018 Jun 18.

Fraunhofer-Chalmers Centre, Chalmers Science Park, Gothenburg, Sweden.

3D bioprinting with cell containing bioinks show great promise in the biofabrication of patient specific tissue constructs. To fulfil the multiple requirements of a bioink, a wide range of materials and bioink composition are being developed and evaluated with regard to cell viability, mechanical performance and printability. It is essential that the printability and printing fidelity is not neglected since failure in printing the targeted architecture may be catastrophic for the survival of the cells and consequently the function of the printed tissue. Read More

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June 2018
4 Reads

High-throughput fabrication of vascularized spheroids for bioprinting.

Biofabrication 2018 06 12;10(3):035009. Epub 2018 Jun 12.

Tissue engineering and Biomaterials Group, Department of Basic Medical Sciences, Faculty of Medicine and Health Sciences, Ghent University, Ghent, Belgium.

Overcoming the problem of vascularization remains the main challenge in the field of tissue engineering. As three-dimensional (3D) bioprinting is the rising technique for the fabrication of large tissue constructs, small prevascularized building blocks were generated that can be incorporated throughout a printed construct, answering the need for a microvasculature within the small micron range (<10 μm). Uniform spheroids with an ideal geometry and diameter for bioprinting were formed, using a high-throughput non-adhesive agarose microwell system. Read More

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June 2018
25 Reads

Pre-set extrusion bioprinting for multiscale heterogeneous tissue structure fabrication.

Biofabrication 2018 06 6;10(3):035008. Epub 2018 Jun 6.

Department of Mechanical System Engineering, Korea Polytechnic University, Siheung, Republic of Korea.

Recent advances in three-dimensional bioprinting technology have led to various attempts in fabricating human tissue-like structures. However, current bioprinting technologies have limitations for creating native tissue-like structures. To resolve these issues, we developed a new pre-set extrusion bioprinting technique that can create heterogeneous, multicellular, and multimaterial structures simultaneously. Read More

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June 2018
8 Reads

Microfluidic system for modelling 3D tumour invasion into surrounding stroma and drug screening.

Biofabrication 2018 06 6;10(3):034102. Epub 2018 Jun 6.

Graduate School at Shenzhen, Tsinghua University, Shenzhen, People's Republic of China.

Tumour invasion into the surrounding stroma is a critical step in metastasis, and it is necessary to clarify the role of microenvironmental factors in tumour invasion. We present a microfluidic system that simulated and controlled multi-factors of the tumour microenvironment for three-dimensional (3D) assessment of tumour invasion into the stroma. The simultaneous, precise and continuous arrangement of two 3D matrices was visualised to observe the migration of cancer cell populations or single cells by transfecting cells with a fluorescent protein. Read More

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June 2018
7 Reads

Bio-resin for high resolution lithography-based biofabrication of complex cell-laden constructs.

Biofabrication 2018 05 11;10(3):034101. Epub 2018 May 11.

Christchurch Regenerative Medicine and Tissue Engineering (CReaTE) Group, Department of Orthopaedic Surgery and Musculoskeletal Medicine, University of Otago Christchurch, Christchurch 8011, New Zealand.

Lithography-based three-dimensional (3D) printing technologies allow high spatial resolution that exceeds that of typical extrusion-based bioprinting approaches, allowing to better mimic the complex architecture of biological tissues. Additionally, lithographic printing via digital light processing (DLP) enables fabrication of free-form lattice and patterned structures which cannot be easily produced with other 3D printing approaches. While significant progress has been dedicated to the development of cell-laden bioinks for extrusion-based bioprinting, less attention has been directed towards the development of cyto-compatible bio-resins and their application in lithography-based biofabrication, limiting the advancement of this promising technology. Read More

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May 2018
10 Reads
4.290 Impact Factor

Photolithographic-stereolithographic-tandem fabrication of 4D smart scaffolds for improved stem cell cardiomyogenic differentiation.

Biofabrication 2018 05 2;10(3):035007. Epub 2018 May 2.

Department of Mechanical and Aerospace Engineering, The George Washington University, Washington DC 20052, United States of America.

4D printing is a highly innovative additive manufacturing process for fabricating smart structures with the ability to transform over time. Significantly different from regular 4D printing techniques, this study focuses on creating novel 4D hierarchical micropatterns using a unique photolithographic-stereolithographic-tandem strategy (PSTS) with smart soybean oil epoxidized acrylate (SOEA) inks for effectively regulating human bone marrow mesenchymal stem cell (hMSC) cardiomyogenic behaviors. The 4D effect refers to autonomous conversion of the surficial-patterned scaffold into a predesigned construct through an external stimulus delivered immediately after printing. Read More

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May 2018
15 Reads

Self-assembled human osseous cell sheets as living biopapers for the laser-assisted bioprinting of human endothelial cells.

Biofabrication 2018 04 30;10(3):035006. Epub 2018 Apr 30.

Centre de recherche en organogénèse expérimentale de l'Université Laval/LOEX, Division of Regenerative Medicine, CHU de Québec Research Center-Université Laval, QC, Canada. Department of Surgery, Faculty of Medicine, Université Laval, Québec, QC, Canada.

A major challenge during the engineering of voluminous bone tissues is to maintain cell viability in the central regions of the construct. In vitro prevascularization of bone substitutes relying on endothelial cell bioprinting has the potential to resolve this issue and to replicate the native bone microvasculature. Laser-assisted bioprinting (LAB) commonly uses biological layers of hydrogel, called 'biopapers', to support patterns of printed cells and constitute the basic units of the construct. Read More

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April 2018
8 Reads

Research and development of 3D printed vasculature constructs.

Biofabrication 2018 04 30;10(3):032002. Epub 2018 Apr 30.

Biomanufacturing and Rapid Forming Technology Key Laboratory of Beijing, Department of Mechanical Engineering, Tsinghua University, Beijing 100084, People's Republic of China.

Artificial blood vessels must be strong, flexible, and must not lead to blockage after implantation. It is therefore important to select an appropriate fabrication process for products to meet these requirements. This review discusses the current methods for making artificial blood vessels, focusing on fabrication principle, materials, and applications. Read More

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April 2018
10 Reads

Matrix stiffness and tumor-associated macrophages modulate epithelial to mesenchymal transition of human adenocarcinoma cells.

Biofabrication 2018 03 28;10(3):035004. Epub 2018 Mar 28.

Translational Medical Oncology Group, Health Research Institute of Santiago de Compostela (IDIS), SERGAS, CIBERONC, E-15706, Santiago de Compostela, Spain.

The tumor microenvironment (TME) is gaining increasing attention in oncology, as it is recognized to be functionally important during tumor development and progression. Tumors are heterogeneous tissues that, in addition to tumor cells, contain tumor-associated cell types such as immune cells, fibroblasts, and endothelial cells. These other cells, together with the specific extracellular matrix (ECM), create a permissive environment for tumor growth. Read More

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March 2018
6 Reads

Laser bioprinting of human induced pluripotent stem cells-the effect of printing and biomaterials on cell survival, pluripotency, and differentiation.

Biofabrication 2018 04 25;10(3):035005. Epub 2018 Apr 25.

REBIRTH-Cluster of Excellence, Hannover Medical School, D-30625, Hannover, Germany. Laser Zentrum Hannover e.V., Nanotechnology Department, D-30419, Hannover, Germany. NIFE-Lower Saxony Centre for Biomedical Engineering, Implant Research and Development, D-30625, Hannover, Germany.

Research on human induced pluripotent stem cells (hiPSCs) is one of the fastest growing fields in biomedicine. Generated from patient's own somatic cells, hiPSCs can be differentiated towards all functional cell types and returned to the patient without immunological concerns. 3D printing of hiPSCs could enable the generation of functional organs for replacement therapies or realization of organ-on-chip systems for individualized medicine. Read More

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April 2018
23 Reads

3D printing strategies for peripheral nerve regeneration.

Biofabrication 2018 03 23;10(3):032001. Epub 2018 Mar 23.

Department of Pathology, Griffith University, Gold Coast, Queensland 4222, Australia. Queensland Eye Institute, 140 Melbourne Street, South Brisbane, Queensland 4101, Australia.

After many decades of biomaterials research for peripheral nerve regeneration, a clinical product (the nerve guide), is emerging as a proven alternative for relatively short injury gaps. This review identifies aspects where 3D printing can assist in improving long-distance nerve guide regeneration strategies. These include (1) 3D printing of the customizable nerve guides, (2) fabrication of scaffolds that fill nerve guides, (3) 3D bioprinting of cells within a matrix/bioink into the nerve guide lumen and the (4) establishment of growth factor gradients along the length a nerve guide. Read More

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March 2018
4 Reads

Construction of a liver sinusoid based on the laminar flow on chip and self-assembly of endothelial cells.

Biofabrication 2018 02 20;10(2):025010. Epub 2018 Feb 20.

Graduate School at Shenzhen, Tsinghua University, Shenzhen, People's Republic of China. Open FIESTA Center, Tsinghua University, Shenzhen 518055, People's Republic of China. Department of Mechanical Engineering and Mechanics, Tsinghua University, Beijing, People's Republic of China.

The liver is one of the main metabolic organs, and nearly all ingested drugs will be metabolized by the liver. Only a small fraction of drugs are able to come onto the market during drug development, and hepatic toxicity is a major cause for drug failure. Since drug development is costly in both time and materials, an in vitro liver model that can accelerate bioreactions in the liver and reduce drug consumption is imperative in the pharmaceutical industry. Read More

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February 2018
6 Reads

Signal-amplifying nanoparticle/hydrogel hybrid microarray biosensor for metal-enhanced fluorescence detection of organophosphorus compounds.

Biofabrication 2018 03 16;10(3):035002. Epub 2018 Mar 16.

Department of Chemical and Biomolecular Engineering, Yonsei University, 50 Yonsei-ro, Seodaemun-gu, Seoul 120-749, Republic of Korea.

In this study, we developed an enzyme-based miniaturized fluorescence biosensor to detect paraoxon, one of the most well-known neurotoxic organophosphorus compounds. The biosensor was fabricated with poly(ethylene glycol) (PEG) hydrogel microarrays that entrapped acetylcholinesterase (AChE) and quantum dots (QDs) as fluorescence reporters. Metal-enhanced fluorescence (MEF) was utilized to amplify the fluorescence signal, which was achieved by decorating QDs on the surface of silica-coated silver nanoparticles (Ag@Silica). Read More

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March 2018
5 Reads

Sprouting angiogenesis in engineered pseudo islets.

Biofabrication 2018 03 16;10(3):035003. Epub 2018 Mar 16.

The Huck Institutes of the Life Sciences, Penn State University, State College, PA 16801, United States of America. Department of Agriculture and Biological Engineering, Penn State University, State College, PA 16801, United States of America.

Despite the recent achievements in cell-based therapies for curing type-1 diabetes (T1D), capillarization in beta (β)-cell clusters is still a major roadblock as it is essential for long-term viability and function of β-cells in vivo. In this research, we report sprouting angiogenesis in engineered pseudo islets (EPIs) made of mouse insulinoma βTC3 cells and rat heart microvascular endothelial cells (RHMVECs). Upon culturing in three-dimensional (3D) constructs under angiogenic conditions, EPIs sprouted extensive capillaries into the surrounding matrix. Read More

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March 2018
14 Reads
4.290 Impact Factor

3D printed Lego-like modular microfluidic devices based on capillary driving.

Biofabrication 2018 03 12;10(3):035001. Epub 2018 Mar 12.

State Key Laboratory of Fluid Power and Mechatronic Systems, School of Mechanical Engineering, Zhejiang University, Hangzhou 310027, People's Republic of China. Key Laboratory of 3D Printing Process and Equipment of Zhejiang Province, School of Mechanical Engineering, Zhejiang University, Hangzhou 310027, People's Republic of China.

The field of how to rapidly assemble microfluidics with modular components continuously attracts researchers' attention, however, extra efforts must be devoted to solving the problems of leaking and aligning between individual modules. This paper presents a novel type of modular microfluidic device, driven by capillary force. There is no necessity for a strict seal or special alignment, and its open structures make it easy to integrate various stents and reactants. Read More

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March 2018
16 Reads

Microspheres containing decellularized cartilage induce chondrogenesis in vitro and remain functional after incorporation within a poly(caprolactone) filament useful for fabricating a 3D scaffold.

Biofabrication 2018 02 2;10(2):025007. Epub 2018 Feb 2.

Department of Orthopaedic Surgery, Cincinnati Children's Hospital Medical Center, Cincinnati, OH 45229, United States of America.

In this study, articular cartilage was decellularized preserving a majority of the inherent proteins, cytokines, growth factors and sGAGs. The decellularized cartilage matrix (dCM) was then encapsulated in poly(lactic acid) microspheres (MS + dCM) via double emulsion. Blank microspheres without dCM, MS(-), were also produced. Read More

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February 2018
4 Reads