541 results match your criteria Biofabrication[Journal]


Optimization of gelatin-alginate composite bioink printability using rheological parameters: a systematic approach.

Biofabrication 2018 Jun 20. Epub 2018 Jun 20.

Institute for Regenerative Medicine, Wake Forest School of Medicine, Medical Center Boulevard, Winston-Salem, North Carolina, 27157, UNITED STATES.

Three-dimensional (3D) bioprinting has emerged as a promising technique in tissue engineering applications through the precise deposition of cells and biomaterials in a layer-by-layer fashion. However, the limited availability of hydrogel bioinks is frequently cited as a major issue for the advancement of cell-based extrusion bioprinting technologies. It is well known that highly viscous materials maintain their structure better, but also have decreased cell viability due to the higher forces which are required for extrusion. Read More

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June 2018
1 Read

3D bioprinting of scaffolds with living Schwann cells for potential nerve tissue engineering applications.

Biofabrication 2018 Jun 18. Epub 2018 Jun 18.

Department of Mechanical Engineering, University of Saskatchewan, 57 Campus Drive, Saskatoon, Saskatchewan, CANADA S7N 5A9, Saskatoon, Saskatchewan, CANADA.

Three-dimensional (3D) bioprinting of biomaterials shows great potential for producing cell-encapsulated scaffolds to repair nerves after injury or disease. For this, preparation of biomaterials and bioprinting itself are critical to create scaffolds with both biological and mechanical properties appropriate for nerve regeneration, yet remain unachievable. This paper presents our study on bioprinting Schwann cell-encapsulated scaffolds using composite hydrogels of alginate, fibrin, hyaluronic acid, and/or RGD peptide, for nerve tissue engineering applications. Read More

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Label free process monitoring of 3D bioprinted engineered constructs via dielectric impedance spectroscopy.

Biofabrication 2018 Jun 14. Epub 2018 Jun 14.

Edward P Fitts Department Industrial and Systems Engineering, North Carolina State University, 406 Daniels Hall, Raleigh, NC 27695, Raleigh, North Carolina, UNITED STATES.

Biofabrication processes can affect biological quality attributes of encapsulated cells within constructs. Currently, assessment of the fabricated constructs is performed offline by subjecting the constructs to destructive assays that require staining and sectioning. This drawback limits the translation of biofabrication processes to industrial practice. Read More

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June 2018
2 Reads

Influence of key processing parameters and seeding density effects of microencapsulated chondrocytes fabricated using electrohydrodynamic spraying.

Biofabrication 2018 Jun 11. Epub 2018 Jun 11.

Trinity Centre for Bioengineering, University of Dublin Trinity College, Dept. of Mechanical Engineering, Parsons Building, Dublin, 2, IRELAND.

Cell delivery and leakage during injection remains a challenge for cell-based intervertebral disc regeneration strategies. Cellular microencapsulation may offer a promising approach to overcome these limitations by providing a protective niche during intradiscal injection. Electrohydrodynamic spraying (EHDS) is a versatile one-step approach for microencapsulation of cells using a high voltage electric field. Read More

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June 2018
4 Reads

Perfusion directed 3D mineral formation within cell-laden hydrogels.

Biofabrication 2018 Jun 8. Epub 2018 Jun 8.

Biomedical and Chemical Engineering, Syracuse University, 318 Bowne Hall, Syracuse, New York, 13244-1200, UNITED STATES.

Despite the promise of stem cell engineering and the new advances in bioprinting technologies, one of the major challenges in the manufacturing of large scale bone tissue scaffolds is the inability to perfuse nutrients throughout thick constructs. Here, we report a scalable method to create thick, perfusable bone constructs using a combination of cell-laden hydrogels and a 3D printed sacrificial polymer. Osteoblast-like Saos-2 cells were encapsulated within a gelatin methacrylate (GelMA) hydrogel and 3D printed polyvinyl alcohol (PVA) pipes were used to create perfusable channels. Read More

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June 2018
1 Read

Tailoring nanostructure and bioactivity of 3D-printable hydrogels with self-assemble peptides amphiphile (PA) for promoting bile duct formation.

Biofabrication 2018 Jun 18;10(3):035010. Epub 2018 Jun 18.

Department of Biomedical Engineering Northwestern University, United States of America.

3D-printing has expanded our ability to produce reproducible and more complex scaffold architectures for tissue engineering applications. In order to enhance the biological response within these 3D-printed scaffolds incorporating nanostructural features and/or specific biological signaling may be an effective means to optimize tissue regeneration. Peptides amphiphiles (PAs) are a versatile supramolecular biomaterial with tailorable nanostructural and biochemical features. Read More

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June 2018
2 Reads

Scaffold-free, label-free and nozzle-free biofabrication technology using magnetic levitational assembly.

Biofabrication 2018 Jun 18;10(3):034104. Epub 2018 Jun 18.

Laboratory for Biotechnological Research '3D Bioprinting Solutions', Moscow, Russia.

Tissue spheroids have been proposed as building blocks in 3D biofabrication. Conventional magnetic force-driven 2D patterning of tissue spheroids requires prior cell labeling by magnetic nanoparticles, meanwhile a label-free approach for 3D magnetic levitational assembly has been introduced. Here we present first time report on rapid assembly of 3D tissue construct using scaffold-free, nozzle-free and label-free magnetic levitation of tissue spheroids. Read More

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June 2018
4 Reads

Bioengineered bile ducts recapitulate key cholangiocyte functions.

Biofabrication 2018 Jun 12;10(3):034103. Epub 2018 Jun 12.

Department of Clinical Sciences of Companion Animals, Faculty of Veterinary Medicine, Utrecht University, The Netherlands. Hubrecht Institute-KNAW and University Medical Center Utrecht, Utrecht, The Netherlands.

Investigation of diseases of the bile duct system and identification of potential therapeutic targets are hampered by the lack of tractable in vitro systems to model cholangiocyte biology. Here, we show a step-wise method for the differentiation of murine Lgr5 liver stem cells (organoids) into cholangiocyte-like cells (CLCs) using a combination of growth factors and extracellular matrix components. Organoid-derived CLCs display key properties of primary cholangiocytes, such as expressing cholangiocyte markers, forming primary cilia, transporting small molecules and responding to farnesoid X receptor agonist. Read More

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June 2018
2 Reads

Simulations of 3D bioprinting: predicting bioprintability of nanofibrillar inks.

Biofabrication 2018 Jun 18;10(3):034105. Epub 2018 Jun 18.

Fraunhofer-Chalmers Centre, Chalmers Science Park, Gothenburg, Sweden.

3D bioprinting with cell containing bioinks show great promise in the biofabrication of patient specific tissue constructs. To fulfil the multiple requirements of a bioink, a wide range of materials and bioink composition are being developed and evaluated with regard to cell viability, mechanical performance and printability. It is essential that the printability and printing fidelity is not neglected since failure in printing the targeted architecture may be catastrophic for the survival of the cells and consequently the function of the printed tissue. Read More

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June 2018
1 Read

High-throughput fabrication of vascularized spheroids for bioprinting.

Biofabrication 2018 Jun 12;10(3):035009. Epub 2018 Jun 12.

Tissue engineering and Biomaterials Group, Department of Basic Medical Sciences, Faculty of Medicine and Health Sciences, Ghent University, Ghent, Belgium.

Overcoming the problem of vascularization remains the main challenge in the field of tissue engineering. As three-dimensional (3D) bioprinting is the rising technique for the fabrication of large tissue constructs, small prevascularized building blocks were generated that can be incorporated throughout a printed construct, answering the need for a microvasculature within the small micron range (<10 μm). Uniform spheroids with an ideal geometry and diameter for bioprinting were formed, using a high-throughput non-adhesive agarose microwell system. Read More

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June 2018
3 Reads

Pre-set extrusion bioprinting for multiscale heterogeneous tissue structure fabrication.

Biofabrication 2018 Jun 6;10(3):035008. Epub 2018 Jun 6.

Department of Mechanical System Engineering, Korea Polytechnic University, Siheung, Republic of Korea.

Recent advances in three-dimensional bioprinting technology have led to various attempts in fabricating human tissue-like structures. However, current bioprinting technologies have limitations for creating native tissue-like structures. To resolve these issues, we developed a new pre-set extrusion bioprinting technique that can create heterogeneous, multicellular, and multimaterial structures simultaneously. Read More

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June 2018
1 Read

Microfluidic system for modelling 3D tumour invasion into surrounding stroma and drug screening.

Biofabrication 2018 Jun 6;10(3):034102. Epub 2018 Jun 6.

Graduate School at Shenzhen, Tsinghua University, Shenzhen, People's Republic of China.

Tumour invasion into the surrounding stroma is a critical step in metastasis, and it is necessary to clarify the role of microenvironmental factors in tumour invasion. We present a microfluidic system that simulated and controlled multi-factors of the tumour microenvironment for three-dimensional (3D) assessment of tumour invasion into the stroma. The simultaneous, precise and continuous arrangement of two 3D matrices was visualised to observe the migration of cancer cell populations or single cells by transfecting cells with a fluorescent protein. Read More

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June 2018
1 Read

Bio-resin for high resolution lithography-based biofabrication of complex cell-laden constructs.

Biofabrication 2018 May 11;10(3):034101. Epub 2018 May 11.

Christchurch Regenerative Medicine and Tissue Engineering (CReaTE) Group, Department of Orthopaedic Surgery and Musculoskeletal Medicine, University of Otago Christchurch, Christchurch 8011, New Zealand.

Lithography-based three-dimensional (3D) printing technologies allow high spatial resolution that exceeds that of typical extrusion-based bioprinting approaches, allowing to better mimic the complex architecture of biological tissues. Additionally, lithographic printing via digital light processing (DLP) enables fabrication of free-form lattice and patterned structures which cannot be easily produced with other 3D printing approaches. While significant progress has been dedicated to the development of cell-laden bioinks for extrusion-based bioprinting, less attention has been directed towards the development of cyto-compatible bio-resins and their application in lithography-based biofabrication, limiting the advancement of this promising technology. Read More

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May 2018
2 Reads

Photolithographic-stereolithographic-tandem fabrication of 4D smart scaffolds for improved stem cell cardiomyogenic differentiation.

Biofabrication 2018 May 2;10(3):035007. Epub 2018 May 2.

Department of Mechanical and Aerospace Engineering, The George Washington University, Washington DC 20052, United States of America.

4D printing is a highly innovative additive manufacturing process for fabricating smart structures with the ability to transform over time. Significantly different from regular 4D printing techniques, this study focuses on creating novel 4D hierarchical micropatterns using a unique photolithographic-stereolithographic-tandem strategy (PSTS) with smart soybean oil epoxidized acrylate (SOEA) inks for effectively regulating human bone marrow mesenchymal stem cell (hMSC) cardiomyogenic behaviors. The 4D effect refers to autonomous conversion of the surficial-patterned scaffold into a predesigned construct through an external stimulus delivered immediately after printing. Read More

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May 2018
3 Reads

Self-assembled human osseous cell sheets as living biopapers for the laser-assisted bioprinting of human endothelial cells.

Biofabrication 2018 Apr 30;10(3):035006. Epub 2018 Apr 30.

Centre de recherche en organogénèse expérimentale de l'Université Laval/LOEX, Division of Regenerative Medicine, CHU de Québec Research Center-Université Laval, QC, Canada. Department of Surgery, Faculty of Medicine, Université Laval, Québec, QC, Canada.

A major challenge during the engineering of voluminous bone tissues is to maintain cell viability in the central regions of the construct. In vitro prevascularization of bone substitutes relying on endothelial cell bioprinting has the potential to resolve this issue and to replicate the native bone microvasculature. Laser-assisted bioprinting (LAB) commonly uses biological layers of hydrogel, called 'biopapers', to support patterns of printed cells and constitute the basic units of the construct. Read More

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April 2018
4 Reads

Research and development of 3D printed vasculature constructs.

Biofabrication 2018 Apr 30;10(3):032002. Epub 2018 Apr 30.

Biomanufacturing and Rapid Forming Technology Key Laboratory of Beijing, Department of Mechanical Engineering, Tsinghua University, Beijing 100084, People's Republic of China.

Artificial blood vessels must be strong, flexible, and must not lead to blockage after implantation. It is therefore important to select an appropriate fabrication process for products to meet these requirements. This review discusses the current methods for making artificial blood vessels, focusing on fabrication principle, materials, and applications. Read More

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April 2018
2 Reads

Matrix stiffness and tumor-associated macrophages modulate epithelial to mesenchymal transition of human adenocarcinoma cells.

Biofabrication 2018 Mar 28;10(3):035004. Epub 2018 Mar 28.

Translational Medical Oncology Group, Health Research Institute of Santiago de Compostela (IDIS), SERGAS, CIBERONC, E-15706, Santiago de Compostela, Spain.

The tumor microenvironment (TME) is gaining increasing attention in oncology, as it is recognized to be functionally important during tumor development and progression. Tumors are heterogeneous tissues that, in addition to tumor cells, contain tumor-associated cell types such as immune cells, fibroblasts, and endothelial cells. These other cells, together with the specific extracellular matrix (ECM), create a permissive environment for tumor growth. Read More

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March 2018
4 Reads

Laser bioprinting of human induced pluripotent stem cells-the effect of printing and biomaterials on cell survival, pluripotency, and differentiation.

Biofabrication 2018 Apr 25;10(3):035005. Epub 2018 Apr 25.

REBIRTH-Cluster of Excellence, Hannover Medical School, D-30625, Hannover, Germany. Laser Zentrum Hannover e.V., Nanotechnology Department, D-30419, Hannover, Germany. NIFE-Lower Saxony Centre for Biomedical Engineering, Implant Research and Development, D-30625, Hannover, Germany.

Research on human induced pluripotent stem cells (hiPSCs) is one of the fastest growing fields in biomedicine. Generated from patient's own somatic cells, hiPSCs can be differentiated towards all functional cell types and returned to the patient without immunological concerns. 3D printing of hiPSCs could enable the generation of functional organs for replacement therapies or realization of organ-on-chip systems for individualized medicine. Read More

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April 2018
9 Reads

3D printing strategies for peripheral nerve regeneration.

Biofabrication 2018 Mar 23;10(3):032001. Epub 2018 Mar 23.

Department of Pathology, Griffith University, Gold Coast, Queensland 4222, Australia. Queensland Eye Institute, 140 Melbourne Street, South Brisbane, Queensland 4101, Australia.

After many decades of biomaterials research for peripheral nerve regeneration, a clinical product (the nerve guide), is emerging as a proven alternative for relatively short injury gaps. This review identifies aspects where 3D printing can assist in improving long-distance nerve guide regeneration strategies. These include (1) 3D printing of the customizable nerve guides, (2) fabrication of scaffolds that fill nerve guides, (3) 3D bioprinting of cells within a matrix/bioink into the nerve guide lumen and the (4) establishment of growth factor gradients along the length a nerve guide. Read More

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March 2018
2 Reads

Construction of a liver sinusoid based on the laminar flow on chip and self-assembly of endothelial cells.

Biofabrication 2018 Feb 20;10(2):025010. Epub 2018 Feb 20.

Graduate School at Shenzhen, Tsinghua University, Shenzhen, People's Republic of China. Open FIESTA Center, Tsinghua University, Shenzhen 518055, People's Republic of China. Department of Mechanical Engineering and Mechanics, Tsinghua University, Beijing, People's Republic of China.

The liver is one of the main metabolic organs, and nearly all ingested drugs will be metabolized by the liver. Only a small fraction of drugs are able to come onto the market during drug development, and hepatic toxicity is a major cause for drug failure. Since drug development is costly in both time and materials, an in vitro liver model that can accelerate bioreactions in the liver and reduce drug consumption is imperative in the pharmaceutical industry. Read More

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February 2018
3 Reads

Signal-amplifying nanoparticle/hydrogel hybrid microarray biosensor for metal-enhanced fluorescence detection of organophosphorus compounds.

Biofabrication 2018 Mar 16;10(3):035002. Epub 2018 Mar 16.

Department of Chemical and Biomolecular Engineering, Yonsei University, 50 Yonsei-ro, Seodaemun-gu, Seoul 120-749, Republic of Korea.

In this study, we developed an enzyme-based miniaturized fluorescence biosensor to detect paraoxon, one of the most well-known neurotoxic organophosphorus compounds. The biosensor was fabricated with poly(ethylene glycol) (PEG) hydrogel microarrays that entrapped acetylcholinesterase (AChE) and quantum dots (QDs) as fluorescence reporters. Metal-enhanced fluorescence (MEF) was utilized to amplify the fluorescence signal, which was achieved by decorating QDs on the surface of silica-coated silver nanoparticles (Ag@Silica). Read More

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March 2018
3 Reads

Sprouting angiogenesis in engineered pseudo islets.

Biofabrication 2018 Mar 16;10(3):035003. Epub 2018 Mar 16.

The Huck Institutes of the Life Sciences, Penn State University, State College, PA 16801, United States of America. Department of Agriculture and Biological Engineering, Penn State University, State College, PA 16801, United States of America.

Despite the recent achievements in cell-based therapies for curing type-1 diabetes (T1D), capillarization in beta (β)-cell clusters is still a major roadblock as it is essential for long-term viability and function of β-cells in vivo. In this research, we report sprouting angiogenesis in engineered pseudo islets (EPIs) made of mouse insulinoma βTC3 cells and rat heart microvascular endothelial cells (RHMVECs). Upon culturing in three-dimensional (3D) constructs under angiogenic conditions, EPIs sprouted extensive capillaries into the surrounding matrix. Read More

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March 2018
7 Reads

3D printed Lego-like modular microfluidic devices based on capillary driving.

Biofabrication 2018 Mar 12;10(3):035001. Epub 2018 Mar 12.

State Key Laboratory of Fluid Power and Mechatronic Systems, School of Mechanical Engineering, Zhejiang University, Hangzhou 310027, People's Republic of China. Key Laboratory of 3D Printing Process and Equipment of Zhejiang Province, School of Mechanical Engineering, Zhejiang University, Hangzhou 310027, People's Republic of China.

The field of how to rapidly assemble microfluidics with modular components continuously attracts researchers' attention, however, extra efforts must be devoted to solving the problems of leaking and aligning between individual modules. This paper presents a novel type of modular microfluidic device, driven by capillary force. There is no necessity for a strict seal or special alignment, and its open structures make it easy to integrate various stents and reactants. Read More

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March 2018
5 Reads

Microspheres containing decellularized cartilage induce chondrogenesis in vitro and remain functional after incorporation within a poly(caprolactone) filament useful for fabricating a 3D scaffold.

Biofabrication 2018 Feb 2;10(2):025007. Epub 2018 Feb 2.

Department of Orthopaedic Surgery, Cincinnati Children's Hospital Medical Center, Cincinnati, OH 45229, United States of America.

In this study, articular cartilage was decellularized preserving a majority of the inherent proteins, cytokines, growth factors and sGAGs. The decellularized cartilage matrix (dCM) was then encapsulated in poly(lactic acid) microspheres (MS + dCM) via double emulsion. Blank microspheres without dCM, MS(-), were also produced. Read More

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February 2018
2 Reads

Proof-of-concept: 3D bioprinting of pigmented human skin constructs.

Biofabrication 2018 Jan 23;10(2):025005. Epub 2018 Jan 23.

Bio-Manufacturing Programme, Singapore Institute of Manufacturing Technology (SIMTech), Agency for Science, Technology and Research (A*STAR), 71 Nanyang Drive, 638075, Singapore. Singapore Centre for 3D Printing (SC3DP), School of Mechanical and Aerospace Engineering, Nanyang Technological University (NTU), 50 Nanyang Avenue, 639798, Singapore.

Three-dimensional (3D) pigmented human skin constructs have been fabricated using a 3D bioprinting approach. The 3D pigmented human skin constructs are obtained from using three different types of skin cells (keratinocytes, melanocytes and fibroblasts from three different skin donors) and they exhibit similar constitutive pigmentation (pale pigmentation) as the skin donors. A two-step drop-on-demand bioprinting strategy facilitates the deposition of cell droplets to emulate the epidermal melanin units (pre-defined patterning of keratinocytes and melanocytes at the desired positions) and manipulation of the microenvironment to fabricate 3D biomimetic hierarchical porous structures found in native skin tissue. Read More

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January 2018
6 Reads

Leaf-templated, microwell-integrated microfluidic chips for high-throughput cell experiments.

Biofabrication 2018 Feb 5;10(2):025008. Epub 2018 Feb 5.

State Key Laboratory for Manufacturing Systems Engineering, Xi'an Jiaotong University, Xi'an 710049, People's Republic of China.

As an alternative to conventional cell culture and animal testing, an organ-on-a-chip is applied to study the biological phenomena of organ development and disease, as well as the interactions between human tissues and external stimuli such as chemicals, forces and electricity. The pattern design of a microfluidic channel is one of the key approaches to regulate cell growth and differentiation, because these channels work as a crucial vasculature system to control the fluidic flow throughout the organ-on-a-chip device. In this study, we introduce a novel leaf-templated, microwell-integrated microfluidic chip for high-throughput cell experiments, consisting of a leaf-venation layer for fluent fluid flow, and a microwell-array layer for cell to reside. Read More

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February 2018
4 Reads

Automated fabrication of photopatterned gelatin hydrogels for organ-on-chips applications.

Biofabrication 2018 Jan 16;10(2):025004. Epub 2018 Jan 16.

Disease Biophysics Group, Wyss Institute for Biologically Inspired Engineering, John A. Paulson School of Engineering and Applied Sciences, Harvard University, Cambridge, MA, United States of America.

Organ-on-chip platforms aim to improve preclinical models for organ-level responses to novel drug compounds. Heart-on-a-chip assays in particular require tissue engineering techniques that rely on labor-intensive photolithographic fabrication or resolution-limited 3D printing of micropatterned substrates, which limits turnover and flexibility of prototyping. We present a rapid and automated method for large scale on-demand micropatterning of gelatin hydrogels for organ-on-chip applications using a novel biocompatible laser-etching approach. Read More

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January 2018
11 Reads

Laser-direct writing by two-photon polymerization of 3D honeycomb-like structures for bone regeneration.

Biofabrication 2018 Feb 5;10(2):025009. Epub 2018 Feb 5.

Faculty of Applied Sciences, University Politehnica of Bucharest, RO-060042, Romania. Center for Advanced Laser Technologies (CETAL), National Institute for Laser, Plasma and Radiation Physics, Magurele, Bucharest, RO-077125, Romania.

A major limitation of existing 3D implantable structures for bone tissue engineering is that most of the cells rapidly attach on the outer edges of the structure, restricting the cells penetration into the inner parts and causing the formation of a necrotic core. Furthermore, these structures generally possess a random spatial arrangement and do not preserve the isotropy on the whole volume. Here, we report on the fabrication and testing of an innovative 3D hierarchical, honeycomb-like structure (HS), with reproducible and isotropic arhitecture, that allows in 'volume' migration of osteoblasts. Read More

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February 2018
7 Reads

A dentin-derived hydrogel bioink for 3D bioprinting of cell laden scaffolds for regenerative dentistry.

Biofabrication 2018 Jan 10;10(2):024101. Epub 2018 Jan 10.

Division of Biomaterials and Biomechanics, Department of Restorative Dentistry, OHSU School of Dentistry, Portland, OR, United States of America.

Recent studies in tissue engineering have adopted extracellular matrix (ECM) derived scaffolds as natural and cytocompatible microenvironments for tissue regeneration. The dentin matrix, specifically, has been shown to be associated with a host of soluble and insoluble signaling molecules that can promote odontogenesis. Here, we have developed a novel bioink, blending printable alginate (3% w/v) hydrogels with the soluble and insoluble fractions of the dentin matrix. Read More

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January 2018
3 Reads

Scalable and physiologically relevant microenvironments for human pluripotent stem cell expansion and differentiation.

Biofabrication 2018 Feb 1;10(2):025006. Epub 2018 Feb 1.

Department of Chemical and Biomolecular Engineering, University of Nebraska, Lincoln, Nebraska, United States of America. Biomedical Engineering Program, University of Nebraska, Lincoln, Nebraska, United States of America.

Human pluripotent stem cells (hPSCs) are required in large numbers for various biomedical applications. However, the scalable and cost-effective culturing of high quality hPSCs and their derivatives remains very challenging. Here, we report a novel and physiologically relevant 3D culture system (called the AlgTube cell culture system) for hPSC expansion and differentiation. Read More

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February 2018
8 Reads

Microspheres containing decellularized cartilage induce chondrogenesis in vitro and remain functional after incorporation within a poly(caprolactone) filament useful for fabricating a 3D scaffold.

Biofabrication 2018 Jan 9. Epub 2018 Jan 9.

Department of Orthopaedic Surgery, Cincinnati Children`s Hospital Medical Center, Cincinnati, Cincinnati, Ohio, UNITED STATES.

In this study, articular cartilage was decellularized preserving a majority of the inherent proteins, cytokines, growth factors and sGAGs. The decellularized cartilage matrix (dCM) was then encapsulated in poly(lactic acid) microspheres (MS+dCM) via double emulsion. Blank microspheres without dCM, MS(-), were also produced. Read More

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January 2018
4 Reads

Direct three-dimensional printing of polymeric scaffolds with nanofibrous topography.

Biofabrication 2018 Jan 12;10(2):025002. Epub 2018 Jan 12.

Division of Regenerative Medicine and Cellular Therapies, School of Pharmacy, University of Nottingham, Nottingham, NG7 2RD, United Kingdom.

Three-dimensional (3D) printing is a powerful manufacturing tool for making 3D structures with well-defined architectures for a wide range of applications. The field of tissue engineering has also adopted this technology to fabricate scaffolds for tissue regeneration. The ability to control architecture of scaffolds, e. Read More

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January 2018
7 Reads

Contact guidance for cardiac tissue engineering using 3D bioprinted gelatin patterned hydrogel.

Biofabrication 2018 Jan 12;10(2):025003. Epub 2018 Jan 12.

Division of Materials Technology, School of Materials Science and Engineering, Nanyang Technological University, 50 Nanyang Avenue 639798, Singapore.

Here, we have developed a 3D bioprinted microchanneled gelatin hydrogel that promotes human mesenchymal stem cell (hMSC) myocardial commitment and supports native cardiomyocytes (CMs) contractile functionality. Firstly, we studied the effect of bioprinted microchanneled hydrogel on the alignment, elongation, and differentiation of hMSC. Notably, the cells displayed well defined F-actin anisotropy and elongated morphology on the microchanneled hydrogel, hence showing the effects of topographical control over cell behavior. Read More

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January 2018
6 Reads

Automated 3D bioassembly of micro-tissues for biofabrication of hybrid tissue engineered constructs.

Biofabrication 2018 Jan 12;10(2):024103. Epub 2018 Jan 12.

Christchurch Regenerative Medicine and Tissue Engineering (CReaTE) Group, Department of Orthopaedic Surgery and Musculoskeletal Medicine, University of Otago Christchurch, Christchurch 8011, New Zealand.

Bottom-up biofabrication approaches combining micro-tissue fabrication techniques with extrusion-based 3D printing of thermoplastic polymer scaffolds are emerging strategies in tissue engineering. These biofabrication strategies support native self-assembly mechanisms observed in developmental stages of tissue or organoid growth as well as promoting cell-cell interactions and cell differentiation capacity. Few technologies have been developed to automate the precise assembly of micro-tissues or tissue modules into structural scaffolds. Read More

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January 2018
3 Reads

Networked concave microwell arrays for constructing 3D cell spheroids.

Biofabrication 2017 Nov 30;10(1):015001. Epub 2017 Nov 30.

KU-KIST Graduate School of Converging Science and Technology, Korea University, Seoul 02841, Republic of Korea.

The engineered three-dimensional (3D) cell cultivation system for the production of multicellular spheroids has attracted considerable attention due to its improved in vivo relevance to cellular communications compared with the traditional two-dimensional (2D) cell culture platform. The formation and maintenance of cell spheroids in a healthy condition is the critical factor for tissue engineering applications such as the repair of damaged tissues, the development of organ replacement parts and preclinical drug tests. However, culturing spheroids in conventional isolated single wells shows limited yield and reduced maintenance periods due to the lack of proper supplies of nutrition as well as intercellular chemical signaling. Read More

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November 2017
3 Reads

One-step delivery of a functional multi-layered cell sheet using a thermally expandable hydrogel with controlled presentation of cell adhesive proteins.

Biofabrication 2018 Jan 10;10(2):025001. Epub 2018 Jan 10.

Department of Bioengineering, Hanyang University, 222 Wangsimri-ro, Seongdong-gu, Seoul 04763, Republic of Korea. BK21 Plus Future Biopharmaceutical Human Resources Training and Research Team, Hanyang University, 222 Wangsimri-ro, Seongdong-gu, Seoul 04763, Republic of Korea.

In this study, we developed a new system enabling rapid delivery of a multi-layered cell sheet by combining layer-by-layer (LBL) coating of a cell membrane and surface engineered thermally expandable hydrogel. Human dermal fibroblasts were LBL-coated with fibronectin (FN) and gelatin to form a multi-layered cell sheet in a single seeding step via spontaneous 3D cell-cell interactions. FN was covalently immobilized onto the surface of a Tetronic-based hydrogel at two different concentrations (1 and 5 μg ml) for stable adhesion of the multi-layered cell sheet, followed by polydopamine coating. Read More

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January 2018
6 Reads

Coaxial extrusion bioprinting of 3D microfibrous constructs with cell-favorable gelatin methacryloyl microenvironments.

Biofabrication 2018 Jan 12;10(2):024102. Epub 2018 Jan 12.

Biomaterials Innovation Research Center, Division of Engineering in Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA 02139, United States of America. Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, MA 02139, United States of America. Key Laboratory of Textile Science and Technology, Ministry of Education, College of Textiles, Donghua University, Shanghai 201620, People's Republic of China.

Bioinks with shear-thinning/rapid solidification properties and strong mechanics are usually needed for the bioprinting of three-dimensional (3D) cell-laden constructs. As such, it remains challenging to generate soft constructs from bioinks at low concentrations that are favorable for cellular activities. Herein, we report a strategy to fabricate cell-laden constructs with tunable 3D microenvironments achieved by bioprinting of gelatin methacryloyl (GelMA)/alginate core/sheath microfibers, where the alginate sheath serves as a template to support and confine the GelMA pre-hydrogel in the core during the extrusion process, allowing for subsequent UV crosslinking. Read More

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January 2018
12 Reads

PLA short sub-micron fiber reinforcement of 3D bioprinted alginate constructs for cartilage regeneration.

Biofabrication 2017 Nov 14;9(4):044105. Epub 2017 Nov 14.

Warsaw University of Technology, Faculty of Materials Science and Engineering, 02-507 Warsaw, Poland.

In this study, we present an innovative strategy to reinforce 3D-printed hydrogel constructs for cartilage tissue engineering by formulating composite bioinks containing alginate and short sub-micron polylactide (PLA) fibers. We demonstrate that Young's modulus obtained for pristine alginate constructs (6.9 ± 1. Read More

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November 2017
4 Reads

Effect of scaffold morphology and cell co-culture on tenogenic differentiation of HADMSC on centrifugal melt electrospun poly (L‑lactic acid) fibrous meshes.

Biofabrication 2017 Nov 14;9(4):044106. Epub 2017 Nov 14.

Mary & Dick Holland Regenerative Medicine Program, University of Nebraska Medical Center, Omaha, NE, United States of America. Division of Cardiology, Department of Internal Medicine, University of Nebraska Medical Center, Omaha, NE, United States of America.

Engineered tendon grafts offer a promising alternative for grafting during the reconstruction of complex tendon tears. The tissue-engineered tendon substitutes have the advantage of increased biosafety and the option to customize their biochemical and biophysical properties to promote tendon regeneration. In this study, we developed a novel centrifugal melt electrospinning (CME) technique, with the goal of optimizing the fabrication parameters to generate fibrous scaffolds for tendon tissue engineering. Read More

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November 2017
3 Reads

Networked concave microwell arrays for constructing 3-D cell spheroids.

Biofabrication 2017 Nov 6. Epub 2017 Nov 6.

KU-KIST Graduate School of Converging Science and Technology, Korea University, 145 Anam-ro, Seongbuk-gu, R&D Center #410, Seoul, 02841, Korea (the Republic of).

The engineered three-dimensional (3-D) cell cultivation system for the production of multicellular spheroids has attracted considerable attention due to its improved in vivo relevance to cellular communications compared to the traditional two-dimensional (2-D) cell culture platform. The formation and maintenance of cell spheroids in healthy condition is the critical factor for tissue engineering applications such as the repair of damaged tissues, the development of organ replacement parts, and preclinical drug tests. However, culturing spheroids in conventional isolated single wells show limit ted yield and maintenance periods due to the lack of proper supplies of nutrition as well as intercellular chemical signaling. Read More

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November 2017
3 Reads

Bioprinted chitosan-gelatin thermosensitive hydrogels using an inexpensive 3D printer.

Biofabrication 2017 Nov 30;10(1):015002. Epub 2017 Nov 30.

School of Chemical Engineering, Oklahoma State University, 420 Engineering North, Stillwater, OK 74078, United States of America.

The primary bottleneck in bioprinting cell-laden structures with carefully controlled spatial relation is a lack of biocompatible inks and printing conditions. In this regard, we explored using thermogelling chitosan-gelatin (CG) hydrogel as a novel bioprinting ink; CG hydrogels are unique in that it undergoes a spontaneous phase change at physiological temperature, and does not need post-processing. In addition, we used a low cost (<$800) compact 3D printer, and modified with a new extruder to print using disposable syringes and hypodermic needles. Read More

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November 2017
4 Reads

Optimization of binding B-lymphocytes in a microfluidic channel: surface modification, stasis time and shear response.

Biofabrication 2017 Nov 30;10(1):014101. Epub 2017 Nov 30.

Future Industries Institute, University of South Australia, Mawson Lakes Campus, Mawson Lakes Boulevard, Mawson Lakes, SA 5095, Australia.

Binding and maintaining cells inside microfluidic channels is a challenging task due to the potential release of cells from the channels with the flow and accompanying shear stress. In this work we optimized the binding of human B-lymphocyte cells (HR1K) inside a microfluidic channel and determined the strength of this binding under shear stress of flowing liquid. In order to determine the parameters required for a live/dead test in microfluidic devices, populations of both living and dead cells were tested separately. Read More

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November 2017
5 Reads

Hypergravity-induced multicellular spheroid generation with different morphological patterns precisely controlled on a centrifugal microfluidic platform.

Biofabrication 2017 Nov 14;9(4):045006. Epub 2017 Nov 14.

Interdisciplinary Program for Bioengineering, Seoul National University Graduate School, Seoul 03087, Republic of Korea.

In living tissue, cells exist in three-dimensional (3D) microenvironments with intricate cell-cell interactions. To model these cellular environments, numerous techniques for generating cell spheroids have been proposed and improved. However, previously reported methods still have limitations in uniformity, reproducibility, scalability, throughput, etc. Read More

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November 2017
22 Reads

Facile and controllable electrochemical fabrication of cell-adhesive polypyrrole electrodes using pyrrole-RGD peptides.

Biofabrication 2017 Nov 14;9(4):045007. Epub 2017 Nov 14.

School of Materials Science and Engineering, Gwangju Institute of Science and Technology, Gwangju 61005, Republic of Korea.

Electrically conductive polymers, such as polypyrrole (PPy), have been widely used for the fabrication of various biosensors and tissue engineering scaffolds. For their biologically relevant applications, conductive biomaterials capable of intimate cellular interactions are highly desired. However, conventional methods to incorporate biomolecules into conductive polymers do not offer fine and easy control over the surface density of the biomolecules and/or their stability. Read More

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November 2017
7 Reads

Bone matrix production in hydroxyapatite-modified hydrogels suitable for bone bioprinting.

Biofabrication 2017 Nov 14;9(4):044103. Epub 2017 Nov 14.

Institute of Interfacial Process Engineering and Plasma Technology, University of Stuttgart, Nobelstraße 12, D-70569 Stuttgart, Germany.

Though bioprinting is a forward-looking approach in bone tissue engineering, the development of bioinks which are on the one hand processable with the chosen printing technique, and on the other hand possess the relevant mechanical as well as osteoconductive features remains a challenge. In the present study, polymer solutions based on methacrylated gelatin and methacrylated hyaluronic acid modified with hydroxyapatite (HAp) particles (5 wt%) were prepared. Encapsulation of primary human adipose-derived stem cells in the HAp-containing gels and culture for 28 d resulted in a storage moduli significantly increased to 126% ± 9. Read More

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November 2017
3 Reads

Recombinant spider silk-based bioinks.

Biofabrication 2017 Nov 14;9(4):044104. Epub 2017 Nov 14.

Lehrstuhl Biomaterialien, Bayreuther Zentrum für Kolloide und Grenzflächen (BZKG), Bayreuther Zentrum für Bio-Makromoleküle (bio-mac), Bayreuther Zentrum für Molekulare Biowissenschaften (BZMB), Bayreuther Materialzentrum (BayMAT), Bayerisches Polymerinstitut (BPI) Universitätsstraße 30, Universität Bayreuth, Bayreuth D-95447, Germany.

Bioinks, 3D cell culture systems which can be printed, are still in the early development stages. Currently, extensive research is going into designing printers to be more accommodating to bioinks, designing scaffolds with stiff materials as support structures for the often soft bioinks, and modifying the bioinks themselves. Recombinant spider silk proteins, a potential biomaterial component for bioinks, have high biocompatibility, can be processed into several morphologies and can be modified with cell adhesion motifs to enhance their bioactivity. Read More

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November 2017
7 Reads

Assessing bioink shape fidelity to aid material development in 3D bioprinting.

Biofabrication 2017 Nov 30;10(1):014102. Epub 2017 Nov 30.

Department of Orthopaedics, University Medical Center Utrecht, Utrecht, The Netherlands.

During extrusion-based bioprinting, the deposited bioink filaments are subjected to deformations, such as collapse of overhanging filaments, which compromises the ability to stack several layers of bioink, and fusion between adjacent filaments, which compromises the resolution and maintenance of a desired pore structure. When developing new bioinks, approaches to assess their shape fidelity after printing would be beneficial to evaluate the degree of deformation of the deposited filament and to estimate how similar the final printed construct would be to the design. However, shape fidelity has been prevalently assessed qualitatively through visual inspection after printing, hampering the direct comparison of the printability of different bioinks. Read More

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November 2017
3 Reads

Automated fabrication of hydrogel microfibers with tunable diameters for controlled cell alignment.

Biofabrication 2017 Nov 14;9(4):045009. Epub 2017 Nov 14.

National Engineering Research Center for Biomaterials, Sichuan University, Sichuan, Chengdu 610064, People's Republic of China.

A newly designed spinning device was utilized to produce continuous hydrogel microfibers with tunable diameters. It was found that the diameter of the microfiber was dependent on perfusion speed and coagulation wheel rotation rate. Their correlation was finally described by a mathematical expression, which proved to be useful for a size-tunable spinning technique. Read More

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November 2017
7 Reads

Creating hierarchical porosity hydroxyapatite scaffolds with osteoinduction by three-dimensional printing and microwave sintering.

Biofabrication 2017 Nov 14;9(4):045008. Epub 2017 Nov 14.

National Engineering Research Center for Biomaterials, Sichuan University, Chengdu, 610064, People's Republic of China.

Hierarchical porosity, which includes micropores and macropores in scaffolds, contributes to important multiple biological functions for tissue regeneration. This paper introduces a two-step method of combining three-dimensional printing (3DP) and microwave sintering to fabricate two-level hierarchical porous scaffolds. The results showed that 3D printing made the macroporous structure well-controlled and microwave sintering generated micropores on the macropore surface. Read More

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November 2017
16 Reads

PLA short sub-micron fibers reinforcement of 3D bioprinted alginate constructs for cartilage regeneration.

Biofabrication 2017 Oct 4. Epub 2017 Oct 4.

Faculty of Materials Science and Engineering, 02507, Warsaw University of Technology, Warsaw, POLAND.

In this study, we present an innovative strategy to reinforce 3D printed hydrogel constructs for cartilage tissue engineering by formulating composite bioinks containing alginate and short sub-micron polylactide (PLA) fibers. We demonstrate that Young's modulus obtained for pristine alginate constructs (6.9 ± 1. Read More

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October 2017
4 Reads