593 results match your criteria Biofabrication[Journal]


Bioprinted osteon-like scaffolds enhance in vivo neovascularization.

Biofabrication 2019 Feb 15. Epub 2019 Feb 15.

Fischell Department of Bioengineering, University of Maryland, college park, Maryland, UNITED STATES.

Bone tissue engineers are facing a daunting challenge when attempting to fabricate bigger constructs intended for use in the treatment of large bone defects, which is the vascularization of the graft. Cell-based approaches and, in particular, the use of in vitro coculture of human umbilical vein endothelial cells (HUVECs) and human mesenchymal stem cells (MSCs) has been one of the most explored options. We present in this paper an alternative method to mimic the spatial pattern of HUVECs and hMSCs found in native osteons based on the use of extrusion-based 3D bioprinting (3DP). Read More

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http://dx.doi.org/10.1088/1758-5090/ab078aDOI Listing
February 2019

ExCeL: Combining Extrusion printing on Cellulose scaffolds with Lamination to create in vitro biological models.

Biofabrication 2019 Feb 15. Epub 2019 Feb 15.

Department of Mechanical Engineering, McMaster University, Center for Advanced Micro-Electro-Fluidics, 1280 Main Street West, Hamilton, Ontario, CANADA L8S 4L7, Hamilton, CANADA.

Bioprinting is rapidly developing to be a powerful tool in tissue engineering for both whole tissue printing and development of in vitro models that can be used in drug discovery, toxicology and as in vitro bioreactors. Nevertheless, the ability to create complex 3D culture systems with different types of cells, extracellular matrices integrated with perfusable channels has been a challenge. Here we develop an approach that combines xurography of a scaffold material (cellulose) with extrusion printing of bioinks on it, followed by assembly in a layer by layer fashion to create complex 3D culture systems that could be used as in vitro models of biological processes. Read More

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http://dx.doi.org/10.1088/1758-5090/ab0798DOI Listing
February 2019

Electrobiofabrication: electrically-based fabrication with biologically-derived materials.

Biofabrication 2019 Feb 13. Epub 2019 Feb 13.

Department of Biological Sciences, University of Maryland, Professor Chemical & Biochemical Engineering, 1000 Hilltop Circle, Baltimore, MD 21250 , USA, college park, UNITED STATES.

While conventional materials fabrication methods focus on form and strength to achieve function, the fabrication of materials systems for emerging life science applications will need to satisfy a more subtle set of requirements. A common goal for biofabrication is to recapitulate complex biological contexts (e.g. Read More

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http://dx.doi.org/10.1088/1758-5090/ab06eaDOI Listing
February 2019
1 Read

Wood-based nanocellulose and bioactive glass modified gelatin-alginate bioinks for 3D bioprinting of bone cells.

Biofabrication 2019 Feb 12. Epub 2019 Feb 12.

Department of Clinical Dentistry, Faculty of Medicine, University of Bergen, Bergen, NORWAY.

A challenge in the extrusion-based bioprinting is to find a bioink with optimal biological and physicochemical properties. The aim of this study was to evaluate the influence of wood-based cellulose nanofibrils (CNF) and bioactive glass on the rheological properties of gelatin-alginate bioinks and the initial responses of bone cells embedded in these inks. CNF modulated the flow behavior of the hydrogels, thus improving their printability. Read More

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http://dx.doi.org/10.1088/1758-5090/ab0692DOI Listing
February 2019
3 Reads

Incorporation of cerium oxide in hollow mesoporous bioglass scaffolds for enhanced bone regeneration by activating ERK signaling pathway.

Biofabrication 2019 Feb 12. Epub 2019 Feb 12.

The Education Ministry Key Lab of Resource Chemistry and Shanghai Key Laboratory of Rare Earth Functional Materials, Shanghai Normal University, No.100 Guilin Rd., Shanghai, 200234, CHINA.

Hierarchically porous structures and bioactive compositions of artificial biomaterials play a positive role in bone defect healing and new bone regeneration. Herein, cerium oxide nanoparticles-modified bioglass (Ce-BG) scaffolds were firstly constructed by the incorporation of hollow mesoporous Ce-BG microspheres in CTS via a freeze-drying technology. The interconnected macropores in Ce-BG scaffolds facilitated the in-growth of bone cells/tissues from material surfaces into the interiors, while the hollow cores and mesopore shells in Ce-BG microspheres provides more active sites for bone mineralization. Read More

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http://dx.doi.org/10.1088/1758-5090/ab0676DOI Listing
February 2019

Microbial transglutaminase induced controlled crosslinking of gelatin methacryloyl to tailor rheological properties for 3D printing.

Biofabrication 2019 Feb 11. Epub 2019 Feb 11.

School of Materials Science and Engineering, Nanyang Technological University, Singapore, SINGAPORE.

Gelatin methacryloyl (GelMA) is a versatile biomaterial that has been shown to possess many advantages such as good biocompatibility, support for cell growth, tunable mechanical properties, photocurable capability, and low material cost. Due to these superior properties, much research has been carried out to develop GelMA as a bioink for bioprinting. However, there are still many challenges, and one major challenge is the control of its rheological properties to yield good printability. Read More

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http://dx.doi.org/10.1088/1758-5090/ab063fDOI Listing
February 2019
4.289 Impact Factor

Printability of pulp derived crystal, fibril and blend nanocellulose-alginate bioinks for extrusion 3D bioprinting.

Biofabrication 2019 Feb 11. Epub 2019 Feb 11.

Reconstructive Surgery and Regenerative Medicine Research Group, Swansea University Medical School, Singleton Campus, Swansea, UNITED KINGDOM OF GREAT BRITAIN AND NORTHERN IRELAND.

Background: One of the main challenges for extrusion 3D bioprinting is the identification of non-synthetic bioinks with suitable rheological properties and biocompatibility. Our aim was to optimise and compare the printability of crystal, fibril and blend formulations of novel pulp derived nanocellulose bioinks and assess biocompatibility with human nasoseptal chondrocytes for cartilage bioprinting. Methods: The printability of crystalline, fibrillated and blend formulations of nanocellulose was determined by assessing resolution (grid-line assay), post-printing shape fidelity and rheology (elasticity, viscosity and shear thinning characteristics) and compared these to pure alginate bioinks. Read More

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http://dx.doi.org/10.1088/1758-5090/ab0631DOI Listing
February 2019
1 Read

Biofabrication strategies for creating microvascular complexity.

Biofabrication 2019 Feb 11. Epub 2019 Feb 11.

Departamento de Ingeniería Mecánica, Universidad Politécnica de Madrid, c/ José Gutiérrez Abascal 2, Madrid, Madrid, 28006, SPAIN.

Design and fabrication of effective biomimetic vasculatures constitutes a relevant and yet unsolved challenge, lying at the heart of tissue repair and regeneration strategies. Even if cell growth is achieved in 3D tissue scaffolds or advanced implants, tissue viability inevitably requires vascularization, as diffusion can only transport nutrients and eliminate debris within a few hundred microns. This engineered vasculature may need to mimic the intricate branching geometry of native microvasculature, referred to herein as vascular complexity, to efficiently deliver blood and recreate critical interactions between the vascular and perivascular cells as well as parenchymal tissues. Read More

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http://dx.doi.org/10.1088/1758-5090/ab0621DOI Listing
February 2019
1 Read

Studies of 3D directed cell migration enabled by direct laser writing of curved wave topography.

Biofabrication 2019 Feb 5. Epub 2019 Feb 5.

Department of Biomedical Engineering, Boston University, Boston, Massachusetts, UNITED STATES.

Cell migration, critical to numerous biological processes, can be guided by surface topography. Studying the effects of topography on cell migration is valuable for enhancing our understanding of directional cell migration and for functionally engineering cell behavior. However, fabrication limitations constrain topography studies to geometries that may not adequately mimic physiological environments. Read More

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http://dx.doi.org/10.1088/1758-5090/ab047fDOI Listing
February 2019
1 Read

Dynamic photopolymerization produces complex microstructures on hydrogels in a moldless approach to generate a 3D intestinal tissue model.

Biofabrication 2019 Feb 5. Epub 2019 Feb 5.

Institut de Bioenginyeria de Catalunya, Barcelona, 08028, SPAIN.

Epithelial tissues contain three-dimensional (3D) complex microtopographies that are essential for proper performance. These microstructures provide cells with the physicochemical cues needed to guide their self-organization into functional tissue structures. However, most in vitro models do not implement these 3D architectural features. Read More

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http://dx.doi.org/10.1088/1758-5090/ab0478DOI Listing
February 2019
2 Reads

Plant seed-inspired cell protection, dormancy, and growth for large-scale Biofabrication.

Biofabrication 2019 Feb 1. Epub 2019 Feb 1.

School of Chemical and Biomedical Engineering, Nanyang Technological University College of Engineering, Singapore, SINGAPORE.

Biofabrication technologies have endowed us with the capability to fabricate complex biological constructs. However, cytotoxic biofabrication conditions have been a major challenge for their clinical application, leading to a trade-off between cell viability and scalability of biofabricated constructs. Taking inspiration from nature, we proposed a cell protection strategy which mimicks the protected and dormant state of plant seeds in adverse external conditions and their germination in response to appropriate environmental cues. Read More

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http://iopscience.iop.org/article/10.1088/1758-5090/ab03ed
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http://dx.doi.org/10.1088/1758-5090/ab03edDOI Listing
February 2019
6 Reads

Process- and bio-inspired hydrogels for 3D bioprinting of soft free-standing neural and glial tissues.

Biofabrication 2019 Jan 29. Epub 2019 Jan 29.

Department of Industrial and Systems Engineering, Virginia Polytechnic Institute and State University, Blacksburg, Virginia, 24061-0131, UNITED STATES.

A bio-inspired hydrogel for 3D bioprinting of soft free-standing neural tissues is presented. The novel filler-free bioinks were designed by combining natural polymers for extracellular matrix biomimicry with synthetic polymers that endow desirable rheological properties for 3D bioprinting. Crosslinking of thiolated Pluronic F-127 with dopamine-conjugated (DC) gelatin and DC hyaluronic acid through a thiol - catechol reaction resulted in thermally gelling bioinks with Herschel-Bulkley fluid rheological behavior. Read More

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http://dx.doi.org/10.1088/1758-5090/ab02c9DOI Listing
January 2019

The fabrication of uniaxially aligned micro-textured polycaprolactone struts and application for skeletal muscle tissue regeneration.

Biofabrication 2019 Feb 5;11(2):025005. Epub 2019 Feb 5.

Department of Biomechatronic Engineering, College of Biotechnology and Bioengineering, Sungkyunkwan University, Suwon, Republic of Korea.

One of the most important factors in skeletal muscle tissue regeneration is the alignment of muscle cells to mimic the native tissue. In this study, we developed a PCL-based scaffold with uniaxially aligned surface topography by stretching a 3D-printed scaffold. We examined the formation of aligned patterns by stretching the samples at different temperatures and stretching rates. Read More

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http://dx.doi.org/10.1088/1758-5090/ab0098DOI Listing
February 2019

Tuning mechanical reinforcement and bioactivity of 3D printed ternary nanocomposites by interfacial peptide-polymer conjugates.

Biofabrication 2019 Jan 15. Epub 2019 Jan 15.

Department of Chemistry, Humboldt-Universität zu Berlin, Berlin, GERMANY.

We present a study on ternary nanocomposites consisting of medical grade poly(ε-caprolactone) (mPCL) matrix, hydroxyapatite nanopowder (nHA) and compatibilized magnesium fluoride nanoparticle (cMgF<sub>2</sub>) fillers. MgF<sub>2</sub> nanoparticles were compatibilized by following a design approach based on the material interfaces of natural bone. MgF<sub>2</sub>-specific peptide-poly(ethylene glycol) conjugates were synthesized and used as surface modifiers for MgF<sub>2</sub> nanoparticles similarly to the non-collagenous proteins (NPC) of bone which compatibilize hydroxyapatite nanocrystallites. Read More

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http://dx.doi.org/10.1088/1758-5090/aafec8DOI Listing
January 2019
1 Read

Programmable higher-order biofabrication of self-locking microencapsulation.

Biofabrication 2019 Jan 9. Epub 2019 Jan 9.

Mechanical and Biomedical Engineering, City University of HongKong, Tat Chee Avenue, Kowloon, HongKong, HONG KONG.

Three-dimensional (3D) hydrogel microcapsules offer great potential in a wide variety of biomedical and tissue engineering applications for their promising biodegradability and customizable geometry. Although recent advances in microfluidics and electrospray techniques have achieved high-throughput production of droplet microcapsules, they still face with the intractable challenge of obtaining programmable shape-engineered microcapsules with complex spatial architecture. Herein, a programmable light-induced biofabrication strategy is proposed to construct higher-order microcapsule architectures by developing a microencapsulation microchip. Read More

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http://dx.doi.org/10.1088/1758-5090/aafd14DOI Listing
January 2019
4 Reads

Bioprinting of 3D breast epithelial spheroids for human cancer models.

Biofabrication 2019 Jan 24;11(2):025003. Epub 2019 Jan 24.

Department of Mechanical Engineering and Mechanics, Drexel University, Philadelphia, PA 19104, United States of America.

3D human cancer models provide a better platform for drug efficacy studies than conventional 2D culture, since they recapitulate important aspects of the in vivo microenvironment. While biofabrication has advanced model creation, bioprinting generally involves extruding individual cells in a bioink and then waiting for these cells to self-assemble into a hierarchical 3D tissue. This self-assembly is time consuming and requires complex cellular interactions with other cell types, extracellular matrix components, and growth factors. Read More

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http://iopscience.iop.org/article/10.1088/1758-5090/aafc49
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http://dx.doi.org/10.1088/1758-5090/aafc49DOI Listing
January 2019
13 Reads

Printomics: the high-throughput analysis of printing parameters applied to melt electrowriting.

Biofabrication 2019 Jan 24;11(2):025004. Epub 2019 Jan 24.

Centre in Regenerative Medicine, Institute of Health and Biomedical Innovation, Queensland University of Technology, 60 Musk Avenue, 4059 Kelvin Grove, Australia.

Melt electrowriting (MEW) combines the fundamental principles of electrospinning, a fibre forming technology, and 3D printing. The process, however, is highly complex and the quality of the fabricated structures strongly depends on the interplay of key printing parameter settings including processing temperature, applied voltage, collection speed, and applied pressure. These parameters act in unison, comprising the principal forces on the electrified jet: pushing the viscous polymer out of the nozzle and mechanically and electrostatically dragging it for deposition towards the collector. Read More

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http://dx.doi.org/10.1088/1758-5090/aafc41DOI Listing
January 2019
2 Reads

Cell-printed 3D liver-on-a-chip possessing a liver microenvironment and biliary system.

Biofabrication 2019 Jan 16;11(2):025001. Epub 2019 Jan 16.

Department of Mechanical Engineering, Pohang University of Science and Technology (POSTECH), San 31, Hyoja-dong, Nam-gu, Pohang, Gyungbuk 790-784, Republic of Korea.

To overcome the drawbacks of in vitro liver testing during drug development, numerous liver-on-a-chip models have been developed. However, current liver-on-a-chip technologies are labor-intensive, lack extracellular matrix (ECM) essential for liver cells, and lack a biliary system essential for excreting bile acids, which contribute to intestinal digestion but are known to be toxic to hepatocytes. Therefore, fabrication methods for development of liver-on-a-chip models that overcome the above limitations are required. Read More

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http://dx.doi.org/10.1088/1758-5090/aaf9faDOI Listing
January 2019
2 Reads

Homogeneous hydroxyapatite/alginate composite hydrogel promotes calcified cartilage matrix deposition with potential for three-dimensional bioprinting.

Biofabrication 2018 12 27;11(1):015015. Epub 2018 Dec 27.

Division of Biomedical Engineering, College of Engineering, University of Saskatchewan, 57 Campus Drive, Saskatoon, Saskatchewan S7N5A9, Canada. Department of Anatomy and Cell Biology, College of Medicine, University of Saskatchewan, 107 Wiggins Road, Saskatoon, Saskatchewan S7N 5E5, Canada.

Calcified cartilage regeneration plays an important role in successful osteochondral repair, since it provides a biological and mechanical transition from the unmineralized cartilage at the articulating surface to the underlying mineralized bone. To biomimic native calcified cartilage in engineered constructs, here we test the hypothesis that hydroxyapatite (HAP) stimulates chondrocytes to secrete the characteristic matrix of calcified cartilage. Sodium citrate (SC) was added as a dispersant of HAP within alginate (ALG), and homogeneous dispersal of HAP within ALG hydrogel was confirmed using sedimentation tests, electron microscopy, and energy dispersive spectroscopy. Read More

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http://dx.doi.org/10.1088/1758-5090/aaf44aDOI Listing
December 2018
2 Reads

Oxygen transporter for the hypoxic transplantation site.

Biofabrication 2018 12 7;11(1):015011. Epub 2018 Dec 7.

Department of Translational Research & Cellular Therapeutics, Beckman Research Institute of City of Hope, 1500 E. Duarte Rd., Duarte, CA 91010, United States of America.

Cell transplantation is a promising treatment for complementing lost function by replacing new cells with a desired function, e.g. pancreatic islet transplantation for diabetics. Read More

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http://stacks.iop.org/1758-5090/11/i=1/a=015011?key=crossref
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http://dx.doi.org/10.1088/1758-5090/aaf2f0DOI Listing
December 2018
7 Reads

Reactive jet impingement bioprinting of high cell density gels for bone microtissue fabrication.

Biofabrication 2018 12 27;11(1):015014. Epub 2018 Dec 27.

School of Engineering, Newcastle University, United Kingdom.

Advances in three-dimensional cell cultures offer new opportunities in biomedical research and drug development. However, there are still challenges to overcome, including the lack of reliability, repeatability and complexity of tissues obtained by these techniques. In this study, we describe a new bioprinting system called reactive jet impingement (ReJI) for the bioprinting of cell-laden hydrogels. Read More

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http://dx.doi.org/10.1088/1758-5090/aaf625DOI Listing
December 2018
1 Read

Fabrication of modular hyaluronan-PEG hydrogels to support 3D cultures of hepatocytes in a perfused liver-on-a-chip device.

Biofabrication 2018 12 27;11(1):015013. Epub 2018 Dec 27.

Division of Biotechnology, Department of Physics, Chemistry and Biology (IFM), Linköping University, Sweden.

Liver cell culture models are attractive in both tissue engineering and for development of assays for drug toxicology research. To retain liver specific cell functions, the use of adequate cell types and culture conditions, such as a 3D orientation of the cells and a proper supply of nutrients and oxygen, are critical. In this article, we show how extracellular matrix mimetic hydrogels can support hepatocyte viability and functionality in a perfused liver-on-a-chip device. Read More

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http://stacks.iop.org/1758-5090/11/i=1/a=015013?key=crossref
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http://dx.doi.org/10.1088/1758-5090/aaf657DOI Listing
December 2018
3 Reads

Fabrication of perfusable 3D hepatic lobule-like constructs through assembly of multiple cell type laden hydrogel microstructures.

Biofabrication 2018 12 27;11(1):015016. Epub 2018 Dec 27.

Intelligent Robotics Institute, School of Mechatronical Engineering, Beijing Institute of Technology, 5 South Zhongguancun Street, Haidian District, Beijing 100081, People's Republic of China.

The in vitro reproduction of three-dimensional (3D) cellular constructs to physiologically mimic human liver is highly desired for drug screening and clinical research. However, the fabrication of a liver-mimetic 3D model using traditional bottom-up technologies is challenging owing to the complex architecture and specific functions of real liver tissue. This work proposes a versatile strategy for spatially assembling gear-like microstructures encapsulating multiple cell types, and reorganizing them into 3D lobule-like micro-architecture with physiological relevance to native liver tissue. Read More

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http://dx.doi.org/10.1088/1758-5090/aaf3c9DOI Listing
December 2018
1 Read

Role and mechanisms of a three-dimensional bioprinted microtissue model in promoting proliferation and invasion of growth-hormone-secreting pituitary adenoma cells.

Biofabrication 2019 Feb 5;11(2):025006. Epub 2019 Feb 5.

Neurosurgical Department, Beijing Tiantan Hospital, Capital Medical University, 6 Tiantan Xili, Dongcheng District, Beijing 100050, People's Republic of China. Beijing Neurosurgical Institute, Capital Medical University, 6 Tiantan Xili, Dongcheng District, Beijing 100050, People's Republic of China.

Growth-hormone-secreting pituitary adenoma (GHSPA) is a benign tumour with a high incidence and large economic burden, which greatly affects quality of life. The aetiological factors are yet to be clarified for GHSPA. Conventional two-dimensional (2D) monolayer culture of tumour cells cannot ideally reflect the growth status of tumours in the physiological environment, and insufficiencies of in vitro models have severely restricted the progress of cancer research. Read More

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http://iopscience.iop.org/article/10.1088/1758-5090/aaf7ea
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http://dx.doi.org/10.1088/1758-5090/aaf7eaDOI Listing
February 2019
3 Reads
4.289 Impact Factor

3D bioprinting of heterogeneous bi- and tri-layered hollow channels within gel scaffolds using scalable multi-axial microfluidic extrusion nozzle.

Biofabrication 2018 12 27;11(1):015012. Epub 2018 Dec 27.

School of Biomedical Engineering, McMaster University, ON, Canada.

One of the primary focuses in recent years in tissue engineering has been the fabrication and integration of vascular structures into artificial tissue constructs. However, most available methodologies lack the ability to create multi-layered concentric conduits inside natural extracellular matrices (ECMs) and gels that replicate more accurately the hierarchical architecture of biological blood vessels. In this work, we present a new microfluidic nozzle design capable of multi-axial extrusion in order to 3D print and pattern bi- and tri-layered hollow channel structures. Read More

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http://dx.doi.org/10.1088/1758-5090/aaf7c7DOI Listing
December 2018
7 Reads

A 3D printed PCL/hydrogel construct with zone-specific biochemical composition mimicking that of the meniscus.

Biofabrication 2019 Jan 16;11(2):025002. Epub 2019 Jan 16.

BIOMATEN, METU Center of Excellence in Biomaterials and Tissue Engineering, Middle East Technical University, Ankara, Turkey. Department of Biological Sciences, Middle East Technical University, Ankara, Turkey. Department of Biotechnology, Middle East Technical University, Ankara, Turkey.

Engineering the meniscus is challenging due to its bizonal structure; the tissue is cartilaginous at the inner portion and fibrous at the outer portion. Here, we constructed an artificial meniscus mimicking the biochemical organization of the native tissue by 3D printing a meniscus shaped PCL scaffold and then impregnating it with agarose (Ag) and gelatin methacrylate (GelMA) hydrogels in the inner and outer regions, respectively. After incubating the constructs loaded with porcine fibrochondrocytes for 8 weeks, we demonstrated that presence of Ag enhanced glycosaminoglycan (GAG) production by about 4 fold (p < 0. Read More

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http://dx.doi.org/10.1088/1758-5090/aaf707DOI Listing
January 2019
2 Reads

Double-layer perfusable collagen microtube device for heterogeneous cell culture.

Biofabrication 2018 11 30;11(1):015010. Epub 2018 Nov 30.

Department of Mechanical Engineering, Faculty of Science and Technology, Keio University, Japan.

In vitro perfusable 3D tissue models mimic in vivo tissues and have several benefits in drug testing. However, processes used to fabricate these models often tend to be complicated. Here, we present a double-layer perfusable collagen tube device for multilayered in vitro 3D cell culture. Read More

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http://dx.doi.org/10.1088/1758-5090/aaf09bDOI Listing
November 2018
1 Read

Porous tissue strands: avascular building blocks for scalable tissue fabrication.

Biofabrication 2018 11 23;11(1):015009. Epub 2018 Nov 23.

Engineering Science and Mechanics Department, The Pennsylvania State University, State College, PA, United States of America. The Huck Institutes of the Life Sciences, The Pennsylvania State University, State College, PA, United States of America.

The scalability of cell aggregates such as spheroids, strands, and rings has been restricted by diffusion of nutrient and oxygen into their core. In this study, we introduce a novel concept in generating tissue building blocks with micropores, which represents an alternative solution for vascularization. Sodium alginate porogens were mixed with human adipose-derived stem cells, and loaded into tubular alginate capsules, followed by de-crosslinking of the capsules. Read More

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http://dx.doi.org/10.1088/1758-5090/aaec22DOI Listing
November 2018
9 Reads

Special issue on bioinks.

Biofabrication 2018 11 23;11(1):010201. Epub 2018 Nov 23.

Department of Functional Materials in Medicine and Dentistry and Bavarian Polymer Institute, University of Würzburg, Pleicherwall 2, D-97070 Würzburg, Germany.

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http://dx.doi.org/10.1088/1758-5090/aaeebcDOI Listing
November 2018
1 Read

A definition of bioinks and their distinction from biomaterial inks.

Biofabrication 2018 11 23;11(1):013001. Epub 2018 Nov 23.

Department of Functional Materials in Medicine and Dentistry and Bavarian Polymer Institute, University of Würzburg, D-97070 Würzburg, Germany.

Biofabrication aims to fabricate biologically functional products through bioprinting or bioassembly (Groll et al 2016 Biofabrication 8 013001). In biofabrication processes, cells are positioned at defined coordinates in three-dimensional space using automated and computer controlled techniques (Moroni et al 2018 Trends Biotechnol. 36 384-402), usually with the aid of biomaterials that are either (i) directly processed with the cells as suspensions/dispersions, (ii) deposited simultaneously in a separate printing process, or (iii) used as a transient support material. Read More

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http://dx.doi.org/10.1088/1758-5090/aaec52DOI Listing
November 2018
10 Reads

Perfusable and stretchable 3D culture system for skin-equivalent.

Biofabrication 2018 11 15;11(1):011001. Epub 2018 Nov 15.

Center for International Research on Integrative Biomedical Systems, Institute of Industrial Science, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo, 153-8505, Japan. Biotechnology Research Institute for Drug Discovery, National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Higashi, Tsukuba, Ibaraki, 305-8565, Japan.

This study describes a perfusable and stretchable culture system for a skin-equivalent. The system is comprised of a flexible culture device equipped with connections that fix vascular channels of the skin-equivalent and functions as an interface for an external pump. Furthermore, a stretching apparatus for the culture device can be fabricated using rapid prototyping technologies, which allows for easy modifications of stretching parameters. Read More

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http://dx.doi.org/10.1088/1758-5090/aaed12DOI Listing
November 2018
1 Read

Reliable autapse formation using the single-cell patterning method.

Biofabrication 2018 11 13;11(1):015008. Epub 2018 Nov 13.

Division of WCU (World Class University) Multiscale Mechanical Design, School of Mechanical and Aerospace Engineering, Seoul National University, Seoul, Republic of Korea.

Auto neuronal synapses, or autapses, are aberrant structures where the synaptic contact of a neuron forms onto its own branch. The functions of autapses, however, remain unknown. Here, we introduce a simple patterning method for capturing a single-cell, in which we maintained the isolated cell until it reached maturity, and developed arrays of autapses for electrophysiological analysis using multi-electrode arrays (MEA). Read More

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http://dx.doi.org/10.1088/1758-5090/aaeb66DOI Listing
November 2018
1 Read

Electrochemical fabrication of a biomimetic elastin-containing bi-layered scaffold for vascular tissue engineering.

Biofabrication 2018 11 9;11(1):015007. Epub 2018 Nov 9.

Department of Chemical Engineering, Florida Institute of Technology, Melbourne, FL 32901, United States of America.

Biomimetic tissue-engineered vascular grafts (TEVGs) have immense potential to replace diseased small-diameter arteries (<4 mm) for the treatment of cardiovascular diseases. However, biomimetic approaches developed thus far only partially recapitulate the physicochemical properties of the native vessel. While it is feasible to fabricate scaffolds that are compositionally similar to native vessels (collagen and insoluble elastic matrix) using freeze-drying, these scaffolds do not mimic the aligned topography of collagen and elastic fibers found in native vessels. Read More

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http://stacks.iop.org/1758-5090/11/i=1/a=015007?key=crossref
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http://dx.doi.org/10.1088/1758-5090/aaeab0DOI Listing
November 2018
8 Reads

StarPEG/heparin-hydrogel based in vivo engineering of stable bizonal cartilage with a calcified bottom layer.

Biofabrication 2018 10 30;11(1):015001. Epub 2018 Oct 30.

Research Centre for Experimental Orthopaedics, Heidelberg University Hospital, Heidelberg, Germany.

Repaired cartilage tissue lacks the typical zonal structure of healthy native cartilage needed for appropriate function. Current grafts for treatment of full thickness cartilage defects focus primarily on a nonzonal design and this may be a reason why inferior nonzonal regeneration tissue developed in vivo. No biomaterial-based solutions have been developed so far to induce a proper zonal architecture into a non-mineralized and a calcified cartilage layer. Read More

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http://dx.doi.org/10.1088/1758-5090/aae75aDOI Listing
October 2018
10 Reads

Co-axial wet-spinning in 3D bioprinting: state of the art and future perspective of microfluidic integration.

Biofabrication 2018 11 9;11(1):012001. Epub 2018 Nov 9.

Department of Chemistry, University of Rome 'La Sapienza', 00185 Rome, Italy. Warsaw University of Technology, Faculty of Materials Science and Engineering, Warsaw, Poland. Institute of Physical Chemistry, Polish Academy of Sciences, 01224 Warsaw, Poland.

Nowadays, 3D bioprinting technologies are rapidly emerging in the field of tissue engineering and regenerative medicine as effective tools enabling the fabrication of advanced tissue constructs that can recapitulate in vitro organ/tissue functions. Selecting the best strategy for bioink deposition is often challenging and time consuming process, as bioink properties-in the first instance, rheological and gelation-strongly influence the suitable paradigms for its deposition. In this short review, we critically discuss one of the available approaches used for bioprinting-namely co-axial wet-spinning extrusion. Read More

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http://iopscience.iop.org/article/10.1088/1758-5090/aae605
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http://dx.doi.org/10.1088/1758-5090/aae605DOI Listing
November 2018
7 Reads

Engineering of microscale vascularized fat that responds to perfusion with lipoactive hormones.

Biofabrication 2018 10 30;11(1):014101. Epub 2018 Oct 30.

Department of Biomedical Engineering, Boston University, Boston, MA 02215, United States of America.

Current methods to treat large soft-tissue defects mainly rely on autologous transfer of adipocutaneous flaps, a method that is often limited by donor site availability. Engineered vascularized adipose tissues can potentially be a viable and readily accessible substitute to autologous flaps. In this study, we engineered a small-scale adipose tissue with pre-patterned vasculature that enables immediate perfusion. Read More

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http://iopscience.iop.org/article/10.1088/1758-5090/aae5fe
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http://dx.doi.org/10.1088/1758-5090/aae5feDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6252090PMC
October 2018
11 Reads

Development of a functional airway-on-a-chip by 3D cell printing.

Biofabrication 2018 10 30;11(1):015002. Epub 2018 Oct 30.

Department of Mechanical Engineering, Pohang University of Science and Technology (POSTECH), 77 Cheongam-ro, Nam-gu, Pohang, Kyungbuk, 37673, Republic of Korea.

We used 3D cell printing to emulate an airway coupled with a naturally-derived blood vessel network in vitro. Decellularized extracellular matrix bioink derived from porcine tracheal mucosa (tmdECM) was used to encapsulate and print endothelial cells and fibroblasts within a designated polycarprolactone (PCL) frame. Providing a niche that emulates conditions in vivo, tmdECM gradually drives endothelial re-orientation, which leads to the formation of a lumen and blood vessel network. Read More

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http://dx.doi.org/10.1088/1758-5090/aae545DOI Listing
October 2018
3 Reads

Optimization of collagen type I-hyaluronan hybrid bioink for 3D bioprinted liver microenvironments.

Biofabrication 2018 10 30;11(1):015003. Epub 2018 Oct 30.

Wake Forest Institute for Regenerative Medicine, Wake Forest School of Medicine, 391 Technology Way, Winston-Salem, NC, 27101, United States of America. Virginia Tech-Wake Forest School of Biomedical Engineering and Sciences, Wake Forest School of Medicine, Medical Center Boulevard, Winston-Salem, NC, 27157, United States of America.

Current 3D printing of tissue is restricted by the use of biomaterials that do not recapitulate the native properties of the extracellular matrix (ECM). These restrictions have thus far prevented optimization of composition and structure of the in vivo tissue microenvironment. The artificial nature of currently used biomaterials affects cellular phenotype and function of the bioprinted tissues, and results in inaccurate modeling of disease and drug metabolism significantly. Read More

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http://iopscience.iop.org/article/10.1088/1758-5090/aae543
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http://dx.doi.org/10.1088/1758-5090/aae543DOI Listing
October 2018
11 Reads

Fabrication of a thick three-dimensional scaffold with an open cellular-like structure using airbrushing and thermal cross-linking of molded short nanofibers.

Biofabrication 2018 11 1;11(1):015006. Epub 2018 Nov 1.

School of Dentistry, The University of Queensland, Oral Health Centre, 288 Herston Rd, QLD 4006, Australia. Department of Engineering Materials and Mechanical Design, Faculty of Engineering, South Valley University, Qena 83523, Egypt.

Nanoscale fibers mimicking the extracellular matrix of natural tissue can be produced by conventional electrospinning, but this approach results in two-dimensional thin dense fibrous mats which can hinder effective cell infiltration. The aim of the present study was to design a thick, three-dimensional (3D) cylindrical scaffold with an open pore structure assembled from short polycaprolactone (PCL) fibers using a facile airbrushing approach. In addition, magnesium particles were incorporated into the PCL solution to both enhance the mechanical properties of the scaffold and stimulate cellular activity following cell seeding. Read More

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http://stacks.iop.org/1758-5090/11/i=1/a=015006?key=crossref
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http://dx.doi.org/10.1088/1758-5090/aae421DOI Listing
November 2018
4 Reads

A novel tissue-engineered 3D tumor model for anti-cancer drug discovery.

Biofabrication 2018 10 30;11(1):015004. Epub 2018 Oct 30.

State Key Laboratory of Fine Chemicals, Dalian R&D Center for Stem Cell and Tissue Engineering, Dalian University of Technology, Dalian 116024, People's Republic of China.

Cancer biology and drug discovery are heavily dependent on conventional 2D cell culture systems. However, a 2D culture is significantly limited by its ability to reflect 'true biology' of tumor in vivo. Three-dimensional (3D) in vitro cell culture models have been introduced to aid cancer drug discovery by better modeling the tumor microenvironment. Read More

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http://dx.doi.org/10.1088/1758-5090/aae270DOI Listing
October 2018
5 Reads

Substrate elasticity dependent colony formation and cardiac differentiation of human induced pluripotent stem cells.

Biofabrication 2018 10 30;11(1):015005. Epub 2018 Oct 30.

École Normale Supérieure-PSL Research University, Département de Chimie, Sorbonne Universités-UPMC Univ Paris 06, CNRS UMR 8640 PASTEUR, 24, rue Lhomond, F-75005 Paris, France.

Substrate elasticity regulates cell functions including cell aggregation and stem cell differentiation. The ability to manufacture substrates of desired elasticity over a broad range is therefore crucial for both fundamental research and advanced applications. In this work, we developed a method to fabricate dense elastomer pillars of different heights on a rigid substrate, providing an effective elasticity ranging from 3 to 168 kPa. Read More

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http://dx.doi.org/10.1088/1758-5090/aae0a5DOI Listing
October 2018
3 Reads
4.290 Impact Factor

3D bioprinting of a hyaluronan bioink through enzymatic-and visible light-crosslinking.

Biofabrication 2018 09 25;10(4):044104. Epub 2018 Sep 25.

AO Research Institute Davos, Davos Platz, Switzerland. Department of Biomaterials Science and Technology, University of Twente, Enschede, The Netherlands.

Extrusion-based three-dimensional bioprinting relies on bioinks engineered to combine viscoelastic properties for extrusion and shape retention, and biological properties for cytocompatibility and tissue regeneration. To satisfy these conflicting requirements, bioinks often utilize either complex mixtures or complex modifications of biopolymers. In this paper we introduce and characterize a bioink exploiting a dual crosslinking mechanism, where an enzymatic reaction forms a soft gel suitable for cell encapsulation and extrusion, while a visible light photo-crosslinking allows shape retention of the printed construct. Read More

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http://dx.doi.org/10.1088/1758-5090/aadf58DOI Listing
September 2018
3 Reads

Bottom-up biofabrication using microfluidic techniques.

Biofabrication 2018 09 17;10(4):044103. Epub 2018 Sep 17.

Institute of Industrial Science, The University of Tokyo, Tokyo, Japan.

Nature builds living organisms in a bottom-up fashion, starting from the expression of genetic information on a cellular level, to the proliferation, differentiation, and self-assembly of cells into tissues/organs during embryo development and wound-healing processes. To mimic this bottom-up approach, it is essential to handle and manipulate small-scale biomaterials using specific technologies, such as microfluidic techniques. Microfluidics provides the tool-sets that deal with the behavior, precise control and manipulation of small amounts of fluids. Read More

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http://dx.doi.org/10.1088/1758-5090/aadef9DOI Listing
September 2018
3 Reads

Peroxidase-catalyzed microextrusion bioprinting of cell-laden hydrogel constructs in vaporized ppm-level hydrogen peroxide.

Biofabrication 2018 09 5;10(4):045007. Epub 2018 Sep 5.

Department of Materials Science and Engineering, Graduate School of Engineering Science, Osaka University, Osaka 560-8531, Japan.

Hydrogels were prepared by contacting air containing 10-50 ppm HO with an aqueous solution containing polymer(s) possessing phenolic hydroxyl (Ph) moieties (polymer-Ph) and horseradish peroxidase (HRP). In this system, HRP catalyzes cross-linking of the Ph moieties by consuming HO diffused from the air. The hydrogelation rate and mechanical properties of the resultant hydrogels can be tuned by controlling the HO concentration in air, the exposure time of the air containing HO to the solution containing polymer-Phs and HRP, and the HRP concentration. Read More

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http://dx.doi.org/10.1088/1758-5090/aadc9eDOI Listing
September 2018
4 Reads

TGF-β induced epithelial-mesenchymal transition in an advanced cervical tumor model by 3D printing.

Biofabrication 2018 09 10;10(4):044102. Epub 2018 Sep 10.

Biomanufacturing Center, Dept. of Mechanical Engineering, Tsinghua University, Haidian District, Beijing 100084, People's Republic of China. Biomanufacturing and Rapid Forming Technology Key Laboratory of Beijing, Beijing 100084, People's Republic of China. Overseas Expertise Introduction Center for Discipline Innovation, Tsinghua University, Haidian District, Beijing 100084, People's Republic of China.

An advanced in vitro cervical tumor model was established by 3D printing to study the epithelial-to-mesenchymal transition (EMT), which is a very important stage of dissemination of carcinoma leading to metastatic tumors. A HeLa/hydrogel grid construct composed of gelatin, alginate, Matrigel and HeLa cells was fabricated by forced extrusion in a layer-by-layer fashion. HeLa cells rapidly proliferated, formed spheroids and presented tumorigenic characteristic in the 3D-printed structure. Read More

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http://dx.doi.org/10.1088/1758-5090/aadbdeDOI Listing
September 2018
3 Reads

Pneumatospinning of collagen microfibers from benign solvents.

Biofabrication 2018 08 14;10(4):045004. Epub 2018 Aug 14.

Embody, Norfolk, VA, United States of America. Eastern Virginia Medical School, Norfolk, VA, United States of America.

Introduction: Current collagen fiber manufacturing methods for biomedical applications, such as electrospinning and extrusion, have had limited success in clinical translation, partially due to scalability, cost, and complexity challenges. Here we explore an alternative, simplified and scalable collagen fiber formation method, termed 'pneumatospinning,' to generate submicron collagen fibers from benign solvents.

Methods And Results: Clinical grade type I atelocollagen from calf corium was electrospun or pneumatospun as sheets of aligned and isotropic fibrous scaffolds. Read More

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http://dx.doi.org/10.1088/1758-5090/aad7d0DOI Listing
August 2018
15 Reads

Biofabrication of human articular cartilage: a path towards the development of a clinical treatment.

Biofabrication 2018 08 21;10(4):045006. Epub 2018 Aug 21.

Department of Surgery, St Vincent's Hospital, University of Melbourne, Clinical Sciences Building, 29 Regent Street, 3065 Fitzroy, VIC, Australia. ARC Centre of Excellence for Electromaterials Science, Intelligent Polymer Research Institute, Innovation Campus, University of Wollongong, NSW, Australia. BioFab3D, Aikenhead Centre for Medical Discovery, St Vincent's Hospital, Melbourne, Australia.

Cartilage injuries cause pain and loss of function, and if severe may result in osteoarthritis (OA). 3D bioprinting is now a tangible option for the delivery of bioscaffolds capable of regenerating the deficient cartilage tissue. Our team has developed a handheld device, the Biopen, to allow in situ additive manufacturing during surgery. Read More

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http://dx.doi.org/10.1088/1758-5090/aad8d9DOI Listing
August 2018
4 Reads

Fabrication of omega-shaped microwell arrays for a spheroid culture platform using pins of a commercial CPU to minimize cell loss and crosstalk.

Biofabrication 2018 08 14;10(4):045003. Epub 2018 Aug 14.

School of Mechanical Engineering, College of Engineering, Chung-Ang University, 84 Heukseok-ro, Dongjak-gu, Seoul 06974, Republic of Korea.

A cell spheroid culture has the benefit of simulating in vivo three-dimensional cell environments. Microwell systems have been developed to mass-produce large quantities of uniform spheroids, and are frequently used in research areas, such as cell biology, anticancer drug development, and regenerative therapy. Recently reported concave-bottomed microwell systems have delivered more benefits in producing spheroids of higher quality and facilitating more effective research. Read More

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http://dx.doi.org/10.1088/1758-5090/aad7d3DOI Listing
August 2018
3 Reads

Soft-molecular imprinted electrospun scaffolds to mimic specific biological tissues.

Biofabrication 2018 08 20;10(4):045005. Epub 2018 Aug 20.

Research Center E. Piaggio and Department of Ingegneria dell'Informazione, University of Pisa, Pisa, Italy. Department of Tissue Regeneration, MIRA Institute for Biomedical Technology and Technical Medicine, Faculty of Science and Technology, University of Twente, Enschede, The Netherlands.

The fabrication of bioactive scaffolds able to mimic the in vivo cellular microenvironment is a challenge for regenerative medicine. The creation of sites for the selective binding of specific endogenous proteins represents an attractive strategy to fabricate scaffolds able to elicit specific cell response. Here, electrospinning (ESP) and soft-molecular imprinting (soft-MI) techniques were combined to fabricate a soft-molecular imprinted electrospun bioactive scaffold (SMIES) for tissue regeneration. Read More

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http://dx.doi.org/10.1088/1758-5090/aad48aDOI Listing
August 2018
6 Reads

Bioprinting of mineralized constructs utilizing multichannel plotting of a self-setting calcium phosphate cement and a cell-laden bioink.

Biofabrication 2018 07 27;10(4):045002. Epub 2018 Jul 27.

Centre for Translational Bone, Joint and Soft Tissue Research, Faculty of Medicine Carl Gustav Carus, Technische Universität Dresden, Dresden, Germany.

Due to their characteristic resemblance of the mineral component of bone, calcium phosphates are widely accepted as optimal bone substitute materials. Recent research focused on the development of pasty calcium phosphate cement (CPC) formulations, which can be fabricated into various shapes by low-temperature extrusion-based additive manufacturing, namely 3D plotting. While it could be demonstrated that sensitive substances like growth factors can be integrated in such printed CPC scaffolds without impairment of their biological activity live cells cannot be suspended in CPC as they may not be functional when enclosed in a solid and stiff matrix. Read More

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http://dx.doi.org/10.1088/1758-5090/aad36dDOI Listing
July 2018
5 Reads