619 results match your criteria Biofabrication[Journal]


Shear-induced alignment of collagen fibrils using 3D cell printing for corneal stroma tissue engineering.

Biofabrication 2019 Apr 17. Epub 2019 Apr 17.

Department of Mechanical Engineering, Pohang University of Science and Technology, Pohang, Kyungbuk 790-784, KOREA, Pohang, KOREA, REPUBLIC OF.

The microenvironments of tissues or organs are complex architectures comprised of structural proteins including collagen. Particularly, the cornea is organized in a lattice pattern of collagen fibrils which play a significant role in its transparency. This paper introduces a transparent bioengineered corneal structure for transplantation. Read More

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http://iopscience.iop.org/article/10.1088/1758-5090/ab1a8b
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http://dx.doi.org/10.1088/1758-5090/ab1a8bDOI Listing
April 2019
4 Reads

Osteogenic and angiogenic tissue formation in high fidelity nanocomposite Laponite-gelatin bioinks.

Biofabrication 2019 Apr 16. Epub 2019 Apr 16.

University of Southampton Institute of Developmental Science, Southampton, Hampshire, UNITED KINGDOM OF GREAT BRITAIN AND NORTHERN IRELAND.

Bioprinting of living cells is rapidly developing as an advanced biofabrication approach to engineer tissues. Bioinks can be extruded in three-dimensions (3D) to fabricate complex and hierarchical constructs for implantation. However, lack of functionality can often be attributed to poor bioink properties. Read More

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http://dx.doi.org/10.1088/1758-5090/ab19fdDOI Listing
April 2019
1 Read

Engineering three-dimensional microenvironments towards in vitro disease models of the central nervous system.

Biofabrication 2019 Apr 9. Epub 2019 Apr 9.

Biomedical Engineering, Hong Kong Polytechnic University, Kowloon, HONG KONG.

The central nervous system (CNS) has a highly complex biophysical and biochemical environment. Despite decades of intensive research, it is still an enormous challenge to restore its functions and regenerate lost or damaged CNS tissues. Current treatment strategies remain sub-optimal because of (1) the hostile microenvironment created post CNS injury, and (2) insufficient understanding of the pathophysiology of acute and chronic CNS diseases. Read More

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http://dx.doi.org/10.1088/1758-5090/ab17aaDOI Listing
April 2019
6 Reads

Electrodeposition-based rapid bioprinting of 3D-designed hydrogels with a pin art device.

Biofabrication 2019 Apr 5. Epub 2019 Apr 5.

Graduate School of Engineering , Tohoku University, Sendai, JAPAN.

Three-dimensional (3D) designed hydrogels are receiving considerable attention for use in tissue engineering. Herein, we present a novel method for bioprinting 3D hydrogels by electrodeposition with a pin art device. The device consists of a metal substrate and an array of electrode pins that can slide independently. Read More

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http://dx.doi.org/10.1088/1758-5090/ab166eDOI Listing
April 2019
1 Read

Ultrasound-assisted biofabrication and bioprinting of preferentially aligned three-dimensional cellular constructs.

Biofabrication 2019 Apr 3. Epub 2019 Apr 3.

Department of Industrial & Systems Engineering, North Carolina State University, Raleigh, North Carolina, UNITED STATES.

A critical consideration in tissue engineering is to recapitulate the microstructural organization of native tissues that is essential to their function. Scaffold-based techniques have focused on achieving this via the contact guidance principle wherein topographical cues offered by scaffold fibers direct migration and orientation of cells to govern subsequent cell-secreted extracellular matrix organization. Alternatively, approaches based on acoustophoretic, electrophoretic, photophoretic, magnetophoretic, and chemotactic principles are being investigated in the biofabrication domain to direct patterning of cells within bioink constructs. Read More

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http://iopscience.iop.org/article/10.1088/1758-5090/ab15cf
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http://dx.doi.org/10.1088/1758-5090/ab15cfDOI Listing
April 2019
4 Reads

3D bioprinting of triphasic nanocomposite hydrogels and scaffolds for cell adhesion and migration.

Biofabrication 2019 Apr 3. Epub 2019 Apr 3.

Univ Munster, Münster, GERMANY.

In this study we describe the first example of 3D (bio)printed triphasic chiral nanocomposite (NC) hydrogels/scaffolds to simulate the complex 3D architecture, nano/micro scale topography, and chiral nature of ECM. These multifunctional constructs are prepared using a 3D (bio)printing technique and are composed of three connected hydrogels/scaffolds, two of which are loaded with nanomaterials functionalized with opposite enantiomers of a biomolecule. With these constructs, we direct the migration of cells toward the part of the triphasic chiral NC hydrogels/scaffolds containing the cells' preferred biomolecule enantiomer. Read More

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http://dx.doi.org/10.1088/1758-5090/ab15caDOI Listing
April 2019
1 Read

3D bioprinted hydrogel model incorporating β-tricalcium phosphate for calcified cartilage tissue engineering.

Biofabrication 2019 Apr 3. Epub 2019 Apr 3.

Faculty of Materials Science and Engineering, 02507, Warsaw University of Technology, Warsaw, POLAND.

One promising strategy to reconstruct osteochondral defects relies on 3D bioprinted three-zonal structures comprised of hyaline cartilage, calcified cartilage, and subchondral bone. So far, several studies have pursued the regeneration of either hyaline cartilage or bone in vitro while - despite its key role in the osteochondral region - only few of them have targeted the calcified layer. In this work, we present a 3D biomimetic hydrogel scaffold containing ß-tricalcium phosphate (TCP) for engineering calcified cartilage through a co-axial needle system implemented in extrusion-based bioprinting process. Read More

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http://dx.doi.org/10.1088/1758-5090/ab15cbDOI Listing
April 2019
1 Read

Cryogenic free-form extrusion bioprinting of decellularized small intestinal submucosa for potential applications in skin tissue engineering.

Biofabrication 2019 Apr 3. Epub 2019 Apr 3.

School of Materials Science and Engineering , Huazhong University of Science and Technology, Wuhan, CHINA.

In current study, a novel strategy of cryogenic 3D bioprinting assisted by free-from extrusion printing was developed and applied to printing of a decellularized small intestinal submucosa (dSIS) slurry. The rheological properties, including kinetic viscosity, storage modulus (G') and loss modulus (G''), were appropriate for free-from extrusion printing of dSIS slurry. Three different groups of scaffolds, including P<sub>500</sub>, P<sub>600</sub>, and P<sub>700</sub>, with filament distance of 500, 600, and 700 µm, respectively were fabricated at 5 mm/s working velocity of platform (Vxy) and 25 kPa air pressure of dispensing system (P) at -20ºC. Read More

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http://dx.doi.org/10.1088/1758-5090/ab15a9DOI Listing
April 2019
1 Read

Engineering small-caliber vascular grafts from collagen filaments and nanofibers with comparable mechanical properties to native vessels.

Biofabrication 2019 Apr 3. Epub 2019 Apr 3.

Wilson College of Textiles, North Carolina State University, Raleigh, North Carolina, UNITED STATES.

At the present time, there is no successful synthetic, off-the-shelf small-caliber vascular graft (< 6 mm) for the repair or bypass of the coronary or carotid arteries. This stimulates on-going investigations to fabricate an artificial vascular graft that has both sufficient mechanical properties as well as superior biological performance. Collagen has long been considered as a viable material to encourage cell recruitment, tissue regeneration, and revascularization, but its use has been limited by its inferior mechanical properties. Read More

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http://dx.doi.org/10.1088/1758-5090/ab15ceDOI Listing
April 2019
1 Read

3D printed dual macro-, microscale porous network as a tissue engineering scaffold with drug delivering function.

Biofabrication 2019 Apr 1. Epub 2019 Apr 1.

University of Melbourne, Melbourne, Victoria, AUSTRALIA.

Tissue engineering macroporous scaffolds are important for regeneration of large volume defects resulting from diseases such as breast or bone cancers. Another important part of the treatment of these conditions is adjuvant drug therapy to prevent disease recurrence or surgical site infection. In this study, we developed a new type of macroporous scaffolds that have drug loading and release functionality to use in these scenarios. Read More

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http://iopscience.iop.org/article/10.1088/1758-5090/ab14ff
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http://dx.doi.org/10.1088/1758-5090/ab14ffDOI Listing
April 2019
9 Reads

Mouse in vitro spermatogenesis on alginate-based 3D bioprinted scaffolds.

Biofabrication 2019 Mar 28. Epub 2019 Mar 28.

Reproduction, Genetics and Regenerative Medicine, Vrije Universiteit Brussel Faculteit Geneeskunde en Farmacie, Brussel, BELGIUM.

In vitro spermatogenesis (IVS) has already been successfully achieved in rodents by organotypic and soft matrix culture systems. However, the former does not allow single cell input, and the latter presents as a simple thick layer in which all cells are embedded. We explored a new culture system using a mouse model by employing an alginate-based hydrogel and 3D bioprinting, to control scaffold design and cell deposition. Read More

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http://dx.doi.org/10.1088/1758-5090/ab1452DOI Listing
March 2019
5 Reads

Recellularization of auricular cartilage via elastase-generated channels.

Biofabrication 2019 Mar 28. Epub 2019 Mar 28.

Erasmus MC, Rotterdam, Zuid-Holland, NETHERLANDS.

Decellularized tissue matrices are promising substrates for tissue generation by stem cells to replace poorly regenerating tissues such as cartilage. However, the dense matrix of decellularized cartilage impedes colonisation by stem cells. Here, we show that digestion of elastin fibre bundles traversing auricular cartilage creates channels through which cells can migrate into the matrix. Read More

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http://dx.doi.org/10.1088/1758-5090/ab1436DOI Listing

Modeling neural circuit, blood-brain barrier, and myelination on a microfluidic 96 well plate.

Biofabrication 2019 Mar 27. Epub 2019 Mar 27.

School of Mechanical and Aerospace Engineering, Seoul National University, Gwanak-gu, Seoul, Korea (the Republic of).

Microfluidics have enabled a wide range of experimental possibilities in the field of neuroscience. Unfortunately, the wider scale adoption of polydimethylsliloxane (PDMS) based microfluidic devices faces challenges due to inherent material compatibility issues and lack of standardized manufacturable devices. In this work, we present an injection molded plastic array 3-dimensional (3D) neuron culture platform (Neuro-IMPACT) made of polystyrene (PS) with a standard 96-well plate form factor that can recapitulate elements of both the central (CNS) and peripheral (PNS) nervous systems. Read More

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http://dx.doi.org/10.1088/1758-5090/ab1402DOI Listing
March 2019
2 Reads
4.289 Impact Factor

Multi-level customized 3D printing for autogenous implants in skull tissue engineering.

Biofabrication 2019 Mar 27. Epub 2019 Mar 27.

Institute of Materials Processing Equipment and Automation, Department of Mechanical Engneering,, Tsinghua University, Beijing, CHINA.

Three-dimensional (3D) printing of decellularized extracellular matrix (ECM) has been achieved to ensure real physiological environments for tissue engineering. However, limited source, biocompatibility and biosafety of decellularized ECM are deficiencies in its large clinical use. Autogenous ECM is biocompatible, bioactive and bio-safe, making it an optimal choice for future clinical applications of 3D printing. Read More

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http://iopscience.iop.org/article/10.1088/1758-5090/ab1400
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http://dx.doi.org/10.1088/1758-5090/ab1400DOI Listing
March 2019
2 Reads
4.289 Impact Factor

Extrusion-based printing of sacrificial Carbopol ink for fabrication of microfluidic devices.

Biofabrication 2019 Apr 16;11(3):034101. Epub 2019 Apr 16.

Engineering Science and Mechanics Department, Penn State University, University Park, PA 16802, United States of America. The Huck Institutes of the Life Sciences, Penn State University, University Park, PA 16802, United States of America. Mechanical Engineering Department, Ceyhan Engineering Faculty, Cukurova University, Adana 01950, Turkey.

Current technologies for manufacturing of microfluidic devices include soft-lithography, wet and dry etching, thermoforming, micro-machining and three-dimensional (3D) printing. Among them, soft-lithography has been the mostly preferred one in medical and pharmaceutical fields due to its ability to generate polydimethylsiloxane (PDMS) devices with resin biocompatibility, throughput and transparency for imaging. It is a multi-step process requiring the preparation of a silicon wafer pattern, which is fabricated using photolithography according to a defined mask. Read More

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http://iopscience.iop.org/article/10.1088/1758-5090/ab10ae
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http://dx.doi.org/10.1088/1758-5090/ab10aeDOI Listing
April 2019
4 Reads
4.289 Impact Factor

Biomimetic design and fabrication of scaffolds integrating oriented micro-pores with branched channel networks for myocardial tissue engineering.

Biofabrication 2019 04 5;11(3):035004. Epub 2019 Apr 5.

Biomanufacturing Center, Department of Mechanical Engineering, Tsinghua University, Beijing 100084, People's Republic of China. Biomanufacturing and Rapid Forming Technology Key Laboratory of Beijing, Beijing 100084, People's Republic of China. 'Biomanufacturing and Engineering Living Systems' Innovation International Talents Base (111 Base), Beijing 100084, People's Republic of China.

The ability to fabricate three-dimensional (3D) thick vascularized myocardial tissue could enable scientific and technological advances in tissue engineering and drug screening, and may accelerate its application in myocardium repair. In this study, we developed a novel biomimetic scaffold integrating oriented micro-pores with branched channel networks to mimic the anisotropy and vasculature of native myocardium. The oriented micro-pores were fabricated using an 'Oriented Thermally Induced Phase Separation (OTIPS)' technique, and the channel network was produced by embedding and subsequently dissolving a 3D-printed carbohydrate template after crosslinking. Read More

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http://dx.doi.org/10.1088/1758-5090/ab0fd3DOI Listing
April 2019
1 Read
4.289 Impact Factor

Femtosecond laser induced densification within cell-laden hydrogels results in cellular alignment.

Biofabrication 2019 04 5;11(3):035005. Epub 2019 Apr 5.

Department of Biomedical and Chemical Engineering, Syracuse University, Syracuse, NY, 13244, United States of America.

The unique capabilities of ultrafast lasers to introduce user-defined microscale modifications within 3D cell-laden hydrogels have been used to investigate fundamental cellular phenomenon such as adhesion, alignment, migration and organization. In this work, we report a new material modification phenomenon coined as 'densification' and its influence on the behavior of encapsulated cells. Femtosecond laser writing technique was used to write densified lines of width 1-5 μm within the bulk of gelatin methacrylate (GelMA) constructs. Read More

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http://dx.doi.org/10.1088/1758-5090/ab0f8bDOI Listing
April 2019
5 Reads

Structural mechanics of 3D-printed poly(lactic acid) scaffolds with tetragonal, hexagonal and wheel-like designs.

Biofabrication 2019 Mar 13. Epub 2019 Mar 13.

Department of Macromolecular Science, Fudan University, Shanghai 200433, Shanghai, CHINA.

While various porous scaffolds have been developed, the focused study about which structure leads to better mechanics is rare. In this study, we designed porous scaffolds with tetragonal, hexagonal and wheel-like structures under a given porosity, and fabricated corresponding poly(lactic acid) (PLA) scaffolds with three-dimensional (3D) printing. High-resolution micro-computed tomography (micro-CT) was carried out to calculate their experimental porosity and confirm their high interconnectivity. Read More

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http://iopscience.iop.org/article/10.1088/1758-5090/ab0f59
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http://dx.doi.org/10.1088/1758-5090/ab0f59DOI Listing
March 2019
6 Reads

3D printing of complex GelMA-based scaffolds with nanoclay.

Biofabrication 2019 04 5;11(3):035006. Epub 2019 Apr 5.

State Key Laboratory of Fluid Power and Mechatronic Systems, School of Mechanical Engineering, Zhejiang University, Hangzhou 310027, People's Republic of China. Key Laboratory of 3D Printing Process and Equipment of Zhejiang Province, School of Mechanical Engineering, Zhejiang University, Hangzhou 310027, People's Republic of China.

Photo-crosslinkable gelatin methacrylate (GelMA) has become an attractive ink in 3D printing due to its excellent biological performance. However, limited by low viscosity and long cross-linking time, it is still a challenge to directly print GelMA by extrusion-based 3D printing. Here, to balance the printability and biocompatibility, biomaterial ink composed of GelMA and nanoclay was specially designed. Read More

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http://dx.doi.org/10.1088/1758-5090/ab0cf6DOI Listing
April 2019
3 Reads
4.289 Impact Factor

Marine-derived natural polymer-based bioprinting ink for biocompatible, durable, and controllable 3D constructs.

Biofabrication 2019 04 1;11(3):035001. Epub 2019 Apr 1.

Department of Chemical Engineering, Pohang University of Science and Technology, Pohang 37673, Republic of Korea.

3D bioprinting (3DBP) is a rapid solid-form fabrication method with a high degree of automation and reproducibility for constructing structural bioscaffolds. However, the development of the 3DBP field has been slowed due to difficulty in acquiring suitable ink materials especially with natural polymers that satisfy all requirements, such as printability, mechanical integrity, and biocompatibility. In this study, a new 3DBP ink of bioengineered sea anemone-derived silk-like protein (aneroin) was used based on its durable mechanical properties and biodegradability in previous studies. Read More

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http://dx.doi.org/10.1088/1758-5090/ab0c6fDOI Listing
April 2019
9 Reads

Fabrication of 3D-nanofibrous fibrinogen scaffolds using salt-induced self assembly.

Biofabrication 2019 03 4;11(2):025010. Epub 2019 Mar 4.

Institute for Biophysics, University of Bremen, Otto-Hahn-Allee 1, D-28359 Bremen, Germany.

Fibrinogen has become highly attractive for tissue engineering scaffolds since it is a naturally occurring blood protein, which contains important binding sites to facilitate cell adhesion. Here, we introduce a novel biofabrication technique to prepare three-dimensional, nanofibrous fibrinogen scaffolds by salt-induced self assembly. For the first time, we were able to fabricate either free-standing or immobilized fibrinogen scaffolds on demand by tailoring the underlying substrate material and adding a fixation and washing procedure after the fiber assembly. Read More

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http://dx.doi.org/10.1088/1758-5090/ab0681DOI Listing
March 2019
1 Read

Evaluation of sterilisation methods for bio-ink components: gelatin, gelatin methacryloyl, hyaluronic acid and hyaluronic acid methacryloyl.

Biofabrication 2019 04 3;11(3):035003. Epub 2019 Apr 3.

BioFab3D, Aikenhead Centre for Medical Discovery, St Vincent's Hospital, Melbourne, Australia. ARC Centre of Excellence for Electromaterials Science, Intelligent Polymer Research Institute, Innovation Campus, University of Wollongong, NSW, Australia.

Reliable and scalable sterilisation of hydrogels is critical to the clinical translation of many biofabrication approaches, such as extrusion-based 3D bioprinting of cell-laden bio-inks. However sterilisation methods can be destructive, and may have detrimental effects on the naturally-derived hydrogels that constitute much of the bio-ink palette. Determining effective sterilisation methods requires detailed analysis of the effects of sterilisation on relevant properties such as viscosity, printability and cytocompatibility. Read More

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http://dx.doi.org/10.1088/1758-5090/ab0b7cDOI Listing
April 2019
4 Reads

Self-assembled DNA hollow spheres from microsponges.

Biofabrication 2019 03 28;11(2):025016. Epub 2019 Mar 28.

Department of Chemical and Biomolecular Engineering, Yonsei University, 50 Yonsei-ro, Seodaemun-gu, Seoul, 03722, Republic of Korea.

We report a novel approach for generating nanosized DNA hollow spheres (HSs) using enzymatically produced DNA microsponges in a self-templating manner. In previous studies, preparation of DNA nanostructures with specified functions required multiple complicated steps. In this study, however, a simple treatment with the nucleophilic agent 4-dimethylaminopyridine (DMAP) enabled a gradual disentanglement of DNA in microsponges by electrostatic interactions between DMAP and DNA, and the DNA underwent a reassembly process to generate hollow shell structures without denaturation/annealing by thermal cycling. Read More

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http://dx.doi.org/10.1088/1758-5090/ab0abbDOI Listing
March 2019
3 Reads

3D bioprinted complex constructs reinforced by hybrid multilayers of electrospun nanofiber sheets.

Biofabrication 2019 03 28;11(2):025015. Epub 2019 Mar 28.

Digital Manufacturing Process Group, Korea Institute of Industrial Technology, 113-58 Seohaean-ro, Siheung-si, Gyeonggi-do, 15014, Republic of Korea. Department of Mechanical Engineering, Hanyang University, Ansan-si, Gyeonggi-do, 15588, Republic of Korea.

Despite the usefulness of hydrogels for cell-based bioprinting, the fragility of their resulting constructs has hindered their practical applications in tissue engineering research. Here, we suggest a hybrid integration method based on cell-hydrogel bioprinting that includes alternate layering of flexible nanofiber (NF) sheets. Because the bioprinting was implemented on a nanofibrous surface, the hydrogel-based materials could be printed with enhanced shape resolution compared to printing on a bare hydrogel. Read More

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http://iopscience.iop.org/article/10.1088/1758-5090/ab08c2
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http://dx.doi.org/10.1088/1758-5090/ab08c2DOI Listing
March 2019
13 Reads

A simple layer-stacking technique to generate biomolecular and mechanical gradients in photocrosslinkable hydrogels.

Biofabrication 2019 03 28;11(2):025014. Epub 2019 Mar 28.

Center for Minimally Invasive Therapeutics (C-MIT), University of California - Los Angeles, Los Angeles, CA, United States of America. Department of Bioengineering, Department of Chemical and Biomolecular Engineering, Henry Samueli School of Engineering and Applied Sciences, University of California - Los Angeles, Los Angeles, CA, United States of America.

Physicochemical and biological gradients are desirable features for hydrogels to enhance their relevance to biological environments for three-dimensional (3D) cell culture. Therefore, simple and efficient techniques to generate chemical, physical and biological gradients within hydrogels are highly desirable. This work demonstrates a technique to generate biomolecular and mechanical gradients in photocrosslinkable hydrogels by stacking and crosslinking prehydrogel solution in a layer by layer manner. Read More

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http://dx.doi.org/10.1088/1758-5090/ab08b5DOI Listing
March 2019
2 Reads
4.289 Impact Factor

Lab-on-a-brane for spheroid formation.

Biofabrication 2019 03 28;11(2):021002. Epub 2019 Mar 28.

CNR-NANOTEC, Institute of Nanotechnology c/o Campus Ecotekne, Lecce, Italy.

Lab-On-a-Brane (LOB) represents a class of Lab-On-a-Chip (LOC) integrating flexible, highly gas permeable and biocompatible thin membranes (TMs). Here we demonstrate the potentiality of LOBs as cell biochips promoting 3D cell growth. The human cancer cells MCF-7 were cultured into standard multiwells (MWs) and into polydimethylsiloxane (PDMS) MWs, LOCs, and LOBs of different wettability. Read More

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http://iopscience.iop.org/article/10.1088/1758-5090/ab0813
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http://dx.doi.org/10.1088/1758-5090/ab0813DOI Listing
March 2019
10 Reads

Bioprinted osteon-like scaffolds enhance in vivo neovascularization.

Biofabrication 2019 03 28;11(2):025013. Epub 2019 Mar 28.

Fischell Department of Bioengineering, University of Maryland, 3121 A James Clark Hall, College Park, MD 20742, United States of America. Center for Engineering Complex Tissues, University of Maryland, 3121 A James Clark Hall, College Park, MD 20742, United States of America.

Bone tissue engineers are facing a daunting challenge when attempting to fabricate bigger constructs intended for use in the treatment of large bone defects, which is the vascularization of the graft. Cell-based approaches and, in particular, the use of in vitro coculture of human umbilical vein endothelial cells (HUVECs) and human mesenchymal stem cells (hMSCs) has been one of the most explored options. We present in this paper an alternative method to mimic the spatial pattern of HUVECs and hMSCs found in native osteons based on the use of extrusion-based 3D bioprinting (3DP). Read More

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http://dx.doi.org/10.1088/1758-5090/ab078aDOI Listing
March 2019
5 Reads

ExCeL: combining extrusion printing on cellulose scaffolds with lamination to create in vitro biological models.

Biofabrication 2019 04 2;11(3):035002. Epub 2019 Apr 2.

School of Biomedical Engineering, McMaster University, Canada.

Bioprinting is rapidly developing into a powerful tool in tissue engineering, for both organ printing and the development of in vitro models that can be used in drug discovery, toxicology and in vitro bioreactors. Nevertheless, the ability to create complex 3D culture systems with different types of cells and extracellular matrices integrated with perfusable channels has been a challenge. Here we develop an approach that combines the xurography of a scaffold material (cellulose) with extrusion printing of bioinks onto it, followed by assembly in a layer-by-layer fashion to create complex 3D culture systems that could be used as in vitro models of biological processes. Read More

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http://dx.doi.org/10.1088/1758-5090/ab0798DOI Listing
April 2019
1 Read

Electrobiofabrication: electrically-based fabrication with biologically-derived materials.

Biofabrication 2019 Feb 13. Epub 2019 Feb 13.

Department of Biological Sciences, University of Maryland, Professor Chemical & Biochemical Engineering, 1000 Hilltop Circle, Baltimore, MD 21250 , USA, college park, UNITED STATES.

While conventional materials fabrication methods focus on form and strength to achieve function, the fabrication of materials systems for emerging life science applications will need to satisfy a more subtle set of requirements. A common goal for biofabrication is to recapitulate complex biological contexts (e.g. Read More

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http://dx.doi.org/10.1088/1758-5090/ab06eaDOI Listing
February 2019
3 Reads

Wood-based nanocellulose and bioactive glass modified gelatin-alginate bioinks for 3D bioprinting of bone cells.

Biofabrication 2019 Feb 12. Epub 2019 Feb 12.

Department of Clinical Dentistry, Faculty of Medicine, University of Bergen, Bergen, NORWAY.

A challenge in the extrusion-based bioprinting is to find a bioink with optimal biological and physicochemical properties. The aim of this study was to evaluate the influence of wood-based cellulose nanofibrils (CNF) and bioactive glass on the rheological properties of gelatin-alginate bioinks and the initial responses of bone cells embedded in these inks. CNF modulated the flow behavior of the hydrogels, thus improving their printability. Read More

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http://dx.doi.org/10.1088/1758-5090/ab0692DOI Listing
February 2019
6 Reads

Incorporation of cerium oxide in hollow mesoporous bioglass scaffolds for enhanced bone regeneration by activating the ERK signaling pathway.

Biofabrication 2019 03 28;11(2):025012. Epub 2019 Mar 28.

The Education Ministry Key Lab of Resource Chemistry and Shanghai Key Laboratory of Rare Earth Functional Materials, Shanghai Normal University, Shanghai 200234, People's Republic of China.

Hierarchically porous structures and bioactive compositions of artificial biomaterials play a positive role in bone defect healing and new bone regeneration. Herein, cerium oxide nanoparticles-modified bioglass (Ce-BG) scaffolds were firstly constructed by the incorporation of hollow mesoporous Ce-BG microspheres in CTS via a freeze-drying technology. The interconnected macropores in Ce-BG scaffolds facilitated the in-growth of bone cells/tissues from material surfaces into the interiors, while the hollow cores and mesopore shells in Ce-BG microspheres provides more active sites for bone mineralization. Read More

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http://dx.doi.org/10.1088/1758-5090/ab0676DOI Listing
March 2019
6 Reads

Microbial transglutaminase induced controlled crosslinking of gelatin methacryloyl to tailor rheological properties for 3D printing.

Biofabrication 2019 03 28;11(2):025011. Epub 2019 Mar 28.

School of Materials Science and Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore 639798, Singapore.

Gelatin methacryloyl (GelMA) is a versatile biomaterial that has been shown to possess many advantages such as good biocompatibility, support for cell growth, tunable mechanical properties, photocurable capability, and low material cost. Due to these superior properties, much research has been carried out to develop GelMA as a bioink for bioprinting. However, there are still many challenges, and one major challenge is the control of its rheological properties to yield good printability. Read More

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http://dx.doi.org/10.1088/1758-5090/ab063fDOI Listing
March 2019
7 Reads
4.289 Impact Factor

Printability of pulp derived crystal, fibril and blend nanocellulose-alginate bioinks for extrusion 3D bioprinting.

Biofabrication 2019 Feb 11. Epub 2019 Feb 11.

Reconstructive Surgery and Regenerative Medicine Research Group, Swansea University Medical School, Singleton Campus, Swansea, UNITED KINGDOM OF GREAT BRITAIN AND NORTHERN IRELAND.

Background: One of the main challenges for extrusion 3D bioprinting is the identification of non-synthetic bioinks with suitable rheological properties and biocompatibility. Our aim was to optimise and compare the printability of crystal, fibril and blend formulations of novel pulp derived nanocellulose bioinks and assess biocompatibility with human nasoseptal chondrocytes for cartilage bioprinting. Methods: The printability of crystalline, fibrillated and blend formulations of nanocellulose was determined by assessing resolution (grid-line assay), post-printing shape fidelity and rheology (elasticity, viscosity and shear thinning characteristics) and compared these to pure alginate bioinks. Read More

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http://dx.doi.org/10.1088/1758-5090/ab0631DOI Listing
February 2019
4 Reads

Biofabrication strategies for creating microvascular complexity.

Biofabrication 2019 Apr 18;11(3):032001. Epub 2019 Apr 18.

Vascular Kinetics Laboratory, Mechanical Engineering & Mechanics, Drexel University, 3141 Chestnut Street, Philadelphia, PA 19104, United States of America.

Design and fabrication of effective biomimetic vasculatures constitutes a relevant and yet unsolved challenge, lying at the heart of tissue repair and regeneration strategies. Even if cell growth is achieved in 3D tissue scaffolds or advanced implants, tissue viability inevitably requires vascularization, as diffusion can only transport nutrients and eliminate debris within a few hundred microns. This engineered vasculature may need to mimic the intricate branching geometry of native microvasculature, referred to herein as vascular complexity, to efficiently deliver blood and recreate critical interactions between the vascular and perivascular cells as well as parenchymal tissues. Read More

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http://dx.doi.org/10.1088/1758-5090/ab0621DOI Listing
April 2019
2 Reads

Studies of 3D directed cell migration enabled by direct laser writing of curved wave topography.

Biofabrication 2019 02 25;11(2):021001. Epub 2019 Feb 25.

Department of Biomedical Engineering, Boston University, Boston, MA 02215, United States of America.

Cell migration, critical to numerous biological processes, can be guided by surface topography. Studying the effects of topography on cell migration is valuable for enhancing our understanding of directional cell migration and for functionally engineering cell behavior. However, fabrication limitations constrain topography studies to geometries that may not adequately mimic physiological environments. Read More

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http://dx.doi.org/10.1088/1758-5090/ab047fDOI Listing
February 2019
12 Reads

Dynamic photopolymerization produces complex microstructures on hydrogels in a moldless approach to generate a 3D intestinal tissue model.

Biofabrication 2019 02 25;11(2):025007. Epub 2019 Feb 25.

Institute for Bioengineering of Catalonia (IBEC), The Barcelona Institute of Science and Technology (BIST), Barcelona, Spain.

Epithelial tissues contain three-dimensional (3D) complex microtopographies that are essential for proper performance. These microstructures provide cells with the physicochemical cues needed to guide their self-organization into functional tissue structures. However, most in vitro models do not implement these 3D architectural features. Read More

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http://dx.doi.org/10.1088/1758-5090/ab0478DOI Listing
February 2019
4 Reads

Plant seed-inspired cell protection, dormancy, and growth for large-scale biofabrication.

Biofabrication 2019 02 25;11(2):025008. Epub 2019 Feb 25.

School of Chemical and Biomedical Engineering, Nanyang Technological University, 70 Nanyang Drive, 639798, Singapore.

Biofabrication technologies have endowed us with the capability to fabricate complex biological constructs. However, cytotoxic biofabrication conditions have been a major challenge for their clinical application, leading to a trade-off between cell viability and scalability of biofabricated constructs. Taking inspiration from nature, we proposed a cell protection strategy which mimicks the protected and dormant state of plant seeds in adverse external conditions and their germination in response to appropriate environmental cues. Read More

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http://iopscience.iop.org/article/10.1088/1758-5090/ab03ed
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http://dx.doi.org/10.1088/1758-5090/ab03edDOI Listing
February 2019
9 Reads

Process- and bio-inspired hydrogels for 3D bioprinting of soft free-standing neural and glial tissues.

Biofabrication 2019 02 25;11(2):025009. Epub 2019 Feb 25.

Department of Industrial and Systems Engineering, Virginia Tech, Blacksburg, VA 24061, United States of America. Macromolecules Innovation Institute, Virginia Tech, Blacksburg, VA 24061, United States of America.

A bio-inspired hydrogel for 3D bioprinting of soft free-standing neural tissues is presented. The novel filler-free bioinks were designed by combining natural polymers for extracellular matrix biomimicry with synthetic polymers to endow desirable rheological properties for 3D bioprinting. Crosslinking of thiolated Pluronic F-127 with dopamine-conjugated (DC) gelatin and DC hyaluronic acid through a thiol-catechol reaction resulted in thermally gelling bioinks with Herschel-Bulkley fluid rheological behavior. Read More

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http://dx.doi.org/10.1088/1758-5090/ab02c9DOI Listing
February 2019
1 Read

The fabrication of uniaxially aligned micro-textured polycaprolactone struts and application for skeletal muscle tissue regeneration.

Biofabrication 2019 02 5;11(2):025005. Epub 2019 Feb 5.

Department of Biomechatronic Engineering, College of Biotechnology and Bioengineering, Sungkyunkwan University, Suwon, Republic of Korea.

One of the most important factors in skeletal muscle tissue regeneration is the alignment of muscle cells to mimic the native tissue. In this study, we developed a PCL-based scaffold with uniaxially aligned surface topography by stretching a 3D-printed scaffold. We examined the formation of aligned patterns by stretching the samples at different temperatures and stretching rates. Read More

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http://dx.doi.org/10.1088/1758-5090/ab0098DOI Listing
February 2019
2 Reads

Tuning mechanical reinforcement and bioactivity of 3D printed ternary nanocomposites by interfacial peptide-polymer conjugates.

Biofabrication 2019 Jan 15. Epub 2019 Jan 15.

Department of Chemistry, Humboldt-Universität zu Berlin, Berlin, GERMANY.

We present a study on ternary nanocomposites consisting of medical grade poly(ε-caprolactone) (mPCL) matrix, hydroxyapatite nanopowder (nHA) and compatibilized magnesium fluoride nanoparticle (cMgF<sub>2</sub>) fillers. MgF<sub>2</sub> nanoparticles were compatibilized by following a design approach based on the material interfaces of natural bone. MgF<sub>2</sub>-specific peptide-poly(ethylene glycol) conjugates were synthesized and used as surface modifiers for MgF<sub>2</sub> nanoparticles similarly to the non-collagenous proteins (NPC) of bone which compatibilize hydroxyapatite nanocrystallites. Read More

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http://dx.doi.org/10.1088/1758-5090/aafec8DOI Listing
January 2019
2 Reads

Programmable higher-order biofabrication of self-locking microencapsulation.

Biofabrication 2019 Jan 9. Epub 2019 Jan 9.

Mechanical and Biomedical Engineering, City University of HongKong, Tat Chee Avenue, Kowloon, HongKong, HONG KONG.

Three-dimensional (3D) hydrogel microcapsules offer great potential in a wide variety of biomedical and tissue engineering applications for their promising biodegradability and customizable geometry. Although recent advances in microfluidics and electrospray techniques have achieved high-throughput production of droplet microcapsules, they still face with the intractable challenge of obtaining programmable shape-engineered microcapsules with complex spatial architecture. Herein, a programmable light-induced biofabrication strategy is proposed to construct higher-order microcapsule architectures by developing a microencapsulation microchip. Read More

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http://dx.doi.org/10.1088/1758-5090/aafd14DOI Listing
January 2019
10 Reads

Bioprinting of 3D breast epithelial spheroids for human cancer models.

Biofabrication 2019 01 24;11(2):025003. Epub 2019 Jan 24.

Department of Mechanical Engineering and Mechanics, Drexel University, Philadelphia, PA 19104, United States of America.

3D human cancer models provide a better platform for drug efficacy studies than conventional 2D culture, since they recapitulate important aspects of the in vivo microenvironment. While biofabrication has advanced model creation, bioprinting generally involves extruding individual cells in a bioink and then waiting for these cells to self-assemble into a hierarchical 3D tissue. This self-assembly is time consuming and requires complex cellular interactions with other cell types, extracellular matrix components, and growth factors. Read More

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http://stacks.iop.org/1758-5090/11/i=2/a=025003?key=crossref
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http://dx.doi.org/10.1088/1758-5090/aafc49DOI Listing
January 2019
34 Reads

Printomics: the high-throughput analysis of printing parameters applied to melt electrowriting.

Biofabrication 2019 01 24;11(2):025004. Epub 2019 Jan 24.

Centre in Regenerative Medicine, Institute of Health and Biomedical Innovation, Queensland University of Technology, 60 Musk Avenue, 4059 Kelvin Grove, Australia.

Melt electrowriting (MEW) combines the fundamental principles of electrospinning, a fibre forming technology, and 3D printing. The process, however, is highly complex and the quality of the fabricated structures strongly depends on the interplay of key printing parameter settings including processing temperature, applied voltage, collection speed, and applied pressure. These parameters act in unison, comprising the principal forces on the electrified jet: pushing the viscous polymer out of the nozzle and mechanically and electrostatically dragging it for deposition towards the collector. Read More

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http://dx.doi.org/10.1088/1758-5090/aafc41DOI Listing
January 2019
3 Reads

Cell-printed 3D liver-on-a-chip possessing a liver microenvironment and biliary system.

Biofabrication 2019 01 16;11(2):025001. Epub 2019 Jan 16.

Department of Mechanical Engineering, Pohang University of Science and Technology (POSTECH), San 31, Hyoja-dong, Nam-gu, Pohang, Gyungbuk 790-784, Republic of Korea.

To overcome the drawbacks of in vitro liver testing during drug development, numerous liver-on-a-chip models have been developed. However, current liver-on-a-chip technologies are labor-intensive, lack extracellular matrix (ECM) essential for liver cells, and lack a biliary system essential for excreting bile acids, which contribute to intestinal digestion but are known to be toxic to hepatocytes. Therefore, fabrication methods for development of liver-on-a-chip models that overcome the above limitations are required. Read More

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http://dx.doi.org/10.1088/1758-5090/aaf9faDOI Listing
January 2019
4 Reads

Homogeneous hydroxyapatite/alginate composite hydrogel promotes calcified cartilage matrix deposition with potential for three-dimensional bioprinting.

Biofabrication 2018 12 27;11(1):015015. Epub 2018 Dec 27.

Division of Biomedical Engineering, College of Engineering, University of Saskatchewan, 57 Campus Drive, Saskatoon, Saskatchewan S7N5A9, Canada. Department of Anatomy and Cell Biology, College of Medicine, University of Saskatchewan, 107 Wiggins Road, Saskatoon, Saskatchewan S7N 5E5, Canada.

Calcified cartilage regeneration plays an important role in successful osteochondral repair, since it provides a biological and mechanical transition from the unmineralized cartilage at the articulating surface to the underlying mineralized bone. To biomimic native calcified cartilage in engineered constructs, here we test the hypothesis that hydroxyapatite (HAP) stimulates chondrocytes to secrete the characteristic matrix of calcified cartilage. Sodium citrate (SC) was added as a dispersant of HAP within alginate (ALG), and homogeneous dispersal of HAP within ALG hydrogel was confirmed using sedimentation tests, electron microscopy, and energy dispersive spectroscopy. Read More

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http://dx.doi.org/10.1088/1758-5090/aaf44aDOI Listing
December 2018
5 Reads

Oxygen transporter for the hypoxic transplantation site.

Biofabrication 2018 12 7;11(1):015011. Epub 2018 Dec 7.

Department of Translational Research & Cellular Therapeutics, Beckman Research Institute of City of Hope, 1500 E. Duarte Rd., Duarte, CA 91010, United States of America.

Cell transplantation is a promising treatment for complementing lost function by replacing new cells with a desired function, e.g. pancreatic islet transplantation for diabetics. Read More

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http://stacks.iop.org/1758-5090/11/i=1/a=015011?key=crossref
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http://dx.doi.org/10.1088/1758-5090/aaf2f0DOI Listing
December 2018
13 Reads

Reactive jet impingement bioprinting of high cell density gels for bone microtissue fabrication.

Biofabrication 2018 12 27;11(1):015014. Epub 2018 Dec 27.

School of Engineering, Newcastle University, United Kingdom.

Advances in three-dimensional cell cultures offer new opportunities in biomedical research and drug development. However, there are still challenges to overcome, including the lack of reliability, repeatability and complexity of tissues obtained by these techniques. In this study, we describe a new bioprinting system called reactive jet impingement (ReJI) for the bioprinting of cell-laden hydrogels. Read More

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http://dx.doi.org/10.1088/1758-5090/aaf625DOI Listing
December 2018
5 Reads

Fabrication of modular hyaluronan-PEG hydrogels to support 3D cultures of hepatocytes in a perfused liver-on-a-chip device.

Biofabrication 2018 12 27;11(1):015013. Epub 2018 Dec 27.

Division of Biotechnology, Department of Physics, Chemistry and Biology (IFM), Linköping University, Sweden.

Liver cell culture models are attractive in both tissue engineering and for development of assays for drug toxicology research. To retain liver specific cell functions, the use of adequate cell types and culture conditions, such as a 3D orientation of the cells and a proper supply of nutrients and oxygen, are critical. In this article, we show how extracellular matrix mimetic hydrogels can support hepatocyte viability and functionality in a perfused liver-on-a-chip device. Read More

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http://stacks.iop.org/1758-5090/11/i=1/a=015013?key=crossref
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http://dx.doi.org/10.1088/1758-5090/aaf657DOI Listing
December 2018
5 Reads

Fabrication of perfusable 3D hepatic lobule-like constructs through assembly of multiple cell type laden hydrogel microstructures.

Biofabrication 2018 12 27;11(1):015016. Epub 2018 Dec 27.

Intelligent Robotics Institute, School of Mechatronical Engineering, Beijing Institute of Technology, 5 South Zhongguancun Street, Haidian District, Beijing 100081, People's Republic of China.

The in vitro reproduction of three-dimensional (3D) cellular constructs to physiologically mimic human liver is highly desired for drug screening and clinical research. However, the fabrication of a liver-mimetic 3D model using traditional bottom-up technologies is challenging owing to the complex architecture and specific functions of real liver tissue. This work proposes a versatile strategy for spatially assembling gear-like microstructures encapsulating multiple cell types, and reorganizing them into 3D lobule-like micro-architecture with physiological relevance to native liver tissue. Read More

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http://dx.doi.org/10.1088/1758-5090/aaf3c9DOI Listing
December 2018
2 Reads

Role and mechanisms of a three-dimensional bioprinted microtissue model in promoting proliferation and invasion of growth-hormone-secreting pituitary adenoma cells.

Biofabrication 2019 02 5;11(2):025006. Epub 2019 Feb 5.

Neurosurgical Department, Beijing Tiantan Hospital, Capital Medical University, 6 Tiantan Xili, Dongcheng District, Beijing 100050, People's Republic of China. Beijing Neurosurgical Institute, Capital Medical University, 6 Tiantan Xili, Dongcheng District, Beijing 100050, People's Republic of China.

Growth-hormone-secreting pituitary adenoma (GHSPA) is a benign tumour with a high incidence and large economic burden, which greatly affects quality of life. The aetiological factors are yet to be clarified for GHSPA. Conventional two-dimensional (2D) monolayer culture of tumour cells cannot ideally reflect the growth status of tumours in the physiological environment, and insufficiencies of in vitro models have severely restricted the progress of cancer research. Read More

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http://iopscience.iop.org/article/10.1088/1758-5090/aaf7ea
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http://dx.doi.org/10.1088/1758-5090/aaf7eaDOI Listing
February 2019
5 Reads
4.289 Impact Factor