749 results match your criteria Biofabrication[Journal]


Scalable microfabrication of drug-loaded core-shell tablets from a single erodible polymer with adjustable release profiles.

Biofabrication 2020 May 28. Epub 2020 May 28.

Department Chemical and Biochemical Engineering, University of Western Ontario, Department of Chemical and Biochemical Engineering, Thompson Engineering Building, Room TEB 439, London, Ontario, N6A 5B9, CANADA.

Oral tablets with tunable release profiles have emerged to enhance the effectiveness of therapies in different clinical conditions. Although the concept of tablets with adjustable release profiles has been studied before, the lack of a fast and scalable production technique has limited their widespread application. In this study, a scalable fabrication method was developed to manufacture controlled-release polyanhydride tablets. Read More

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http://dx.doi.org/10.1088/1758-5090/ab97a0DOI Listing

Direct-write 3D printing and characterization of a GelMA-based biomaterial for intracorporeal tissue engineering.

Biofabrication 2020 May 28. Epub 2020 May 28.

Mechanical and Aerospace Engineering, The Ohio State University, Columbus, Ohio, UNITED STATES.

We develop and characterize a biomaterial formulation and robotic methods tailored for intracorporeal tissue engineering (TE) via direct-write (DW) 3D printing. Intracorporeal TE is defined as the biofabrication of 3D TE scaffolds inside of a living patient, in a minimally invasive manner. A biomaterial for intracorporeal TE requires to be 3D printable and crosslinkable via mechanisms that are safe to native tissues and feasible at physiological temperature (37 °C). Read More

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http://dx.doi.org/10.1088/1758-5090/ab97a1DOI Listing

Trapping cell spheroids and organoids using digital acoustofluidics.

Biofabrication 2020 May 21. Epub 2020 May 21.

Intelligent Systems Engineering , Indiana University Bloomington, Bloomington, Indiana, UNITED STATES.

The precise positioning and arrangement of cell spheroids and organoids are critical to reconstructing complex tissue architecture for tissue engineering and regenerative medicine. Here, we present a digital acoustofluidic method to manipulate cell spheroids and organoids with unprecedented dexterity. By introducing localized vibrations via a C-shaped integrated digital transducer (IDT), we can generate a trapping node to immobilize cell spheroids with a diameter ranging from 20 μm to 300 μm. Read More

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http://dx.doi.org/10.1088/1758-5090/ab9582DOI Listing

3D bioprinting using hollow multifunctional fiber impedimetric sensors.

Biofabrication 2020 May 20. Epub 2020 May 20.

Department of Industrial and Systems Engineering, Virginia Polytechnic Institute and State University, Blacksburg, Virginia, 24061-0131, UNITED STATES.

3D bioprinting is an emerging biofabrication process for the production of adherent cell-based products, including engineered tissues and foods. While process innovations are rapidly occurring in the area of process monitoring, which can improve fundamental understanding of process-structure-property relations as well as product quality by closed-loop control techniques, in-line sensing of the bioink composition remains a challenge. Here, we present that hollow multifunctional fibers enable in-line impedimetric sensing of bioink composition and exhibit selectivity for real-time classification of cell type, viability, and state of differentiation during bioprinting. Read More

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http://dx.doi.org/10.1088/1758-5090/ab94d0DOI Listing

A composite hydrogel-3D printed thermoplast osteochondral anchor as an example for a zonal approach to cartilage repair: in vivo performance in a long-term equine model.

Biofabrication 2020 May 20. Epub 2020 May 20.

Utrecht University, Utrecht, Utrecht, NETHERLANDS.

Recent research has been focusing on the generation of living personalized osteochondral constructs for joint repair. Native articular cartilage has a zonal structure, which is not reflected in current constructs and which may be a cause of the frequent failure of these repair attempts. Therefore, we investigated the performance of a composite implant that further reflects the zonal distribution of cellular component both in vitro and in vivo in a long-term equine model. Read More

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http://dx.doi.org/10.1088/1758-5090/ab94ceDOI Listing

4D biofabrication of fibrous artificial nerve graft for neuron regeneration.

Biofabrication 2020 May 20. Epub 2020 May 20.

University of Bayreuth, Bayreuth, 95440, GERMANY.

In this paper, we describe the application of the 4D biofabrication approach for the fabrication of artificial nerve graft. Bilayer scaffolds consisting of uniaxially aligned polycaprolactone-poly(glycerol sebacate) (PCL-PGS) and randomly aligned methacrylated hyaluronic acid (HA-MA) fibers were fabricated using electrospinning and further used for the culture of PC-12 neuron cells. Tubular structures form instantly after immersion of fibrous bilayer in an aqueous buffer and the diameter of obtained tubes can be controlled by changing bilayer parameters such as the thickness of each layer, overall bilayer thickness, and medium counterion concentration. Read More

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http://dx.doi.org/10.1088/1758-5090/ab94cfDOI Listing

Dynamic peptide-folding mediated biofunctionalization and modulation of hydrogels for 4D bioprinting.

Biofabrication 2020 May 19. Epub 2020 May 19.

Department of Physics, Chemistry, and Biology, Linkopings universitet, Linkoping, SWEDEN.

Hydrogels are used in a wide range of biomedical applications, including three-dimensional (3D) cell culture, cell therapy and bioprinting. To enable processing using advanced additive fabrication techniques and to mimic the dynamic nature of the extracellular matrix (ECM), the properties of the hydrogels must be possible to tailor and change over time with high precision. The design of hydrogels that are both structurally and functionally dynamic, while providing necessary mechanical support is challenging using conventional synthesis techniques. Read More

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http://dx.doi.org/10.1088/1758-5090/ab9490DOI Listing

The effect of BMP-mimetic peptide tethering bioinks on the differentiation of dental pulp stem cells (DPSCs) in 3D bioprinted dental constructs.

Biofabrication 2020 May 19. Epub 2020 May 19.

Wake Forest Institute for Regenerative Medicine, Wake Forest School of Medicine, Winston-Salem, North Carolina, UNITED STATES.

The goal of this study was to use 3D bioprinting technology to create a bioengineered dental construct containing human dental pulp stem cells (hDPSCs). To accomplish this, we first developed a novel bone morphogenetic protein (BMP) peptide-tethering bioink formulation and examined its rheological properties, its printability, and the structural stability of the bioprinted construct. Second, we evaluated the survival and differentiation of hDPSCs in the bioprinted dental construct by measuring cell viability, proliferation, and gene expression, as well as histological and immunofluorescent analyses. Read More

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http://dx.doi.org/10.1088/1758-5090/ab9492DOI Listing

Use of inkjet-printed single cells to quantify intratumoral heterogeneity.

Biofabrication 2020 May 19. Epub 2020 May 19.

Department of Creative IT Engineering, Pohang University of Science and Technology, Pohang, Gyeongsangbuk-do, Korea (the Republic of).

Quantification of intratumoural heterogeneity is essential for designing effective therapeutic strategies in the age of personalized medicine. In this study, we used a piezoelectric inkjet printer to enable analysis of intratumoral heterogeneity in a bladder cancer for the first time. Patient derived tumor organoids were dissociated into single cell suspension and used as a bioink. Read More

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http://dx.doi.org/10.1088/1758-5090/ab9491DOI Listing

Single-cell-level screening method for migratory cancer cells and its potential feasibility in high-throughput manner.

Biofabrication 2020 May 14. Epub 2020 May 14.

Department of Bioengineering, University of Texas at Arlington, 500 UTA blvd ERB 244, USA, Arlington, Texas, 76010, UNITED STATES.

High-throughput screening (HTS) is a well-established approach for tumor-specific drug development because of its high efficiency and customizable selection of antineoplastic drugs. However, there is still a lack of an appropriate cell-based HTS specific for migratory cancer cells. In the study presented here, we created a novel assay (mHTS): a single-cell-level screening method targeting migratory cancer cells and can be applied in a high-throughput manner. Read More

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http://dx.doi.org/10.1088/1758-5090/ab9315DOI Listing

3D printed composite scaffolds with dual small molecule delivery for mandibular bone regeneration.

Biofabrication 2020 May 5. Epub 2020 May 5.

Regenerative Medicine, University of Nebraska Medical Center, DRCII R6035, Omaha, Nebraska, 68198-7400, UNITED STATES.

Functional reconstruction of craniomaxillofacial defects is challenging, especially for the patients who suffer from traumatic injury, cranioplasty, and oncologic surgery. Three-dimensional (3D) printing/bioprinting technologies provide a promising tool to fabricate bone tissue engineering constructs with complex architectures and bioactive components. In this study, we implemented multi-material 3D printing to fabricate 3D printed PCL/hydrogel composite scaffolds loaded with dual bioactive small molecules (i. Read More

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http://dx.doi.org/10.1088/1758-5090/ab906eDOI Listing

Functional evaluation of prevascularization in one-stage versus two-stage tissue engineering approach of human bio-artificial muscle.

Biofabrication 2020 May 1. Epub 2020 May 1.

Department of Development and Regeneration, Katholieke Universiteit Leuven, E. Sabbelaan 53, Kortrijk, W-Vl, 8500, BELGIUM.

A common shortcoming of current tissue engineered constructs is the lack of a functional vasculature, limiting their size and functionality. Prevascularization is a possible strategy to introduce vascular networks in these constructs. It includes among others co-culturing target cells with endothelial (precursor) cells that are able to form endothelial networks through vasculogenesis. Read More

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http://dx.doi.org/10.1088/1758-5090/ab8f36DOI Listing

Polydopamine regulated hydroxyapatite microspheres grown in the three-dimensional honeycomb-like mollusk shell-derived organic template for osteogenesis.

Biofabrication 2020 Apr 30. Epub 2020 Apr 30.

Huazhong University of Science and Technology, Wuhan, Hubei, CHINA.

Layered osteochondral composite scaffolds are considered as a promising strategy for the treatment of osteochondral defects. However, the insufficient osseous support and integration of the subchondral bone layer frequently result in the failure of osteochondral repair. Therefore, it is essentially important to explore new subchondral bone constructs tailored to support bone integration and healing. Read More

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http://dx.doi.org/10.1088/1758-5090/ab8f20DOI Listing

Freeform 3D printing using a continuous viscoelastic supporting matrix.

Biofabrication 2020 May 15;12(3):035017. Epub 2020 May 15.

Department of Chemistry, CICECO-Aveiro Institute of Materials, University of Aveiro, Campus Universitário de Santiago, Aveiro, 3810-193, Portugal. These authors contributed equally to this work. Authors to whom any correspondence should be addressed.

Embedded bio-printing has fostered significant advances toward the fabrication of soft complex tissue-like constructs, by providing a physical support that allows the freeform shape maintenance within the prescribed spatial arrangement, even under gravity force. Current supporting materials still present major drawbacks for up-scaling embedded 3D bio-printing technology towards tissue-like constructs with clinically relevant dimensions. Herein, we report a a cost-effective and widely available supporting material for embedded bio-printing consisting on a continuous pseudo-plastic matrix of xanthan-gum (XG). Read More

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http://dx.doi.org/10.1088/1758-5090/ab8bc3DOI Listing
May 2020
4.289 Impact Factor

High throughput direct 3D bioprinting in multiwell plates.

Biofabrication 2020 Apr 16. Epub 2020 Apr 16.

Nanoengineering, University of California San Diego, La Jolla, California, UNITED STATES.

Advances in three dimensional (3D) bioprinting have enabled the fabrication of sophisticated 3D tissue scaffolds for biological and medical applications, where high speed, high throughput production in well plates is a critical need. Here, we present an integrated 3D bioprinting platform based on microscale continuous optical printing, capable of high throughput in situ rapid fabrication of complex 3D biomedical samples in multiwell plate formats for subsequent culture and analysis. Our high throughput 3D bioprinter (HT-3DP) was used to showcase constructs of varying spatial geometries of biomimetic significance, tunable mechanical properties, as well as reproducibility. Read More

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http://dx.doi.org/10.1088/1758-5090/ab89caDOI Listing

Cell-laden microfibers fabricated using μL cell-suspension.

Biofabrication 2020 Apr 16. Epub 2020 Apr 16.

Institute of Industrial Science, University of Tokyo, The University of Tokyo, Komaba, Meguro-ku,, Tokyo 153-8505, Tokyo, Tokyo, 153-8505, JAPAN.

Current microfluidic methods for cell-laden microfiber fabrication generally require larger than 100 μL of cell-suspensions. Since some "rare" cells can be only acquired in a small amount, the preparation of >100 μL cell-suspensions with high-cell density can be both expensive and time-consuming. Here, we present a facile method capable of fabricating cell-laden microfibers using small-volume cell-suspensions. Read More

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http://dx.doi.org/10.1088/1758-5090/ab89cbDOI Listing

Microfluidics in biofabrication.

Biofabrication 2020 Apr 16;12(3):030201. Epub 2020 Apr 16.

Faculty of Materials Science and Engineering, Warsaw University of Technology, Warsaw, Poland. Authors to whom any correspondence should be addressed.

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http://dx.doi.org/10.1088/1758-5090/ab7e75DOI Listing
April 2020
4.289 Impact Factor

Hyaluronic acid as a bioink for extrusion-based 3D printing.

Biofabrication 2020 May 28;12(3):032001. Epub 2020 May 28.

AO Research Institute Davos, Davos Platz, Switzerland. Department of Biomaterials Science and Technology, University of Twente, Enschede, The Netherlands.

Biofabrication is enriching the tissue engineering field with new ways of producing structurally organized complex tissues. Among the numerous bioinks under investigation, hyaluronic acid (HA) and its derivatives stand out for their biological relevance, cytocompatibility, shear-thinning properties, and potential to fine-tune the desired properties with chemical modification. In this paper, we review the recent advances on bioinks containing HA. Read More

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http://dx.doi.org/10.1088/1758-5090/ab8752DOI Listing

Nanoclay-based 3D printed scaffolds promote vascular ingrowth ex vivo and generate bone mineral tissue in vitro and in vivo.

Biofabrication 2020 May 12;12(3):035010. Epub 2020 May 12.

Bone and Joint Research Group, Centre for Human Development, Stem Cells and Regeneration, Institute of Developmental Sciences, University of Southampton, Southampton, United Kingdom. Center for Life Nano Science, Istituto Italiano di Tecnologia, Viale Regina Elena 291, 00161 Rome, Italy.

Acellular soft hydrogels are not ideal for hard tissue engineering given their poor mechanical stability, however, in combination with cellular components offer significant promise for tissue regeneration. Indeed, nanocomposite bioinks provide an attractive platform to deliver human bone marrow stromal cells (HBMSCs) in three dimensions producing cell-laden constructs that aim to facilitate bone repair and functionality. Here we present the in vitro, ex vivo and in vivo investigation of bioprinted HBMSCs encapsulated in a nanoclay-based bioink to produce viable and functional three-dimensional constructs. Read More

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http://dx.doi.org/10.1088/1758-5090/ab8753DOI Listing

Reinforcing interpenetrating network hydrogels with 3D printed polymer networks to engineer cartilage mimetic composites.

Biofabrication 2020 May 12;12(3):035011. Epub 2020 May 12.

Trinity Centre for Biomedical Engineering, Trinity Biomedical Sciences Institute, Trinity College Dublin, Dublin, Ireland. Department of Mechanical and Manufacturing Engineering, School of Engineering, Trinity College Dublin, Ireland.

Engineering constructs that mimic the complex structure, composition and biomechanics of the articular cartilage represents a promising route to joint regeneration. Such tissue engineering strategies require the development of biomaterials that mimic the mechanical properties of articular cartilage whilst simultaneously providing an environment supportive of chondrogenesis. Here three-dimensional (3D) bioprinting is used to develop polycaprolactone (PCL) fibre networks to mechanically reinforce interpenetrating network (IPN) hydrogels consisting of alginate and gelatin methacryloyl (GelMA). Read More

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http://dx.doi.org/10.1088/1758-5090/ab8708DOI Listing

Machine learning-based design strategy for 3D printable bioink: elastic modulus and yield stress determine printability.

Biofabrication 2020 May 28;12(3):035018. Epub 2020 May 28.

Center for Biomaterials, Biomedical Research Institute, Korea Institute of Science and Technology, Seoul 02792, Republic of Korea.

Although three-dimensional (3D) bioprinting technology is rapidly developing, the design strategies for biocompatible 3D-printable bioinks remain a challenge. In this study, we developed a machine learning-based method to design 3D-printable bioink using a model system with naturally derived biomaterials. First, we demonstrated that atelocollagen (AC) has desirable physical properties for printing compared to native collagen (NC). Read More

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http://dx.doi.org/10.1088/1758-5090/ab8707DOI Listing

Biofabrication of nerve fibers with mimetic myelin sheath-like structure and aligned fibrous niche.

Biofabrication 2020 May 12;12(3):035013. Epub 2020 May 12.

Engineering Research Center in Biomaterials, Sichuan University, Chengdu, Sichuan 610064 People's Republic of China.

Nerve tissues contain hierarchically ordered nerve fibers, while each of the nerve fibers has nano-oriented fibrous extracellular matrix and a core-shell structure of tubular myelin sheath with elongated axons encapsulated. Here, we report, for the first time, a ready approach to fabricate biomimetic nerve fibers which are oriented and have a core-shell structure to spatially encapsulate two types of cells, neurons and Schwann cells. A microfluidic system was designed and assembled, which contained a coaxial triple-channel chip and a stretching loading device. Read More

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http://dx.doi.org/10.1088/1758-5090/ab860dDOI Listing
May 2020
4.289 Impact Factor

In-situ re-melting and re-solidification treatment of selective laser sintered polycaprolactone lattice scaffolds for improved filament quality and mechanical properties.

Biofabrication 2020 May 15;12(3):035012. Epub 2020 May 15.

State Key Laboratory for Manufacturing Systems Engineering, Xi'an Jiaotong University, Xi'an 710049, People's Republic of China. Rapid manufacturing research center of Shaanxi Province, Xi'an Jiaotong University, Xi'an 710049, People's Republic of China.

Selective laser sintering (SLS) is a promising additive manufacturing technique that produces biodegradable tissue-engineered scaffolds with highly porous architectures without additional supporting. However, SLS process inherently results in partially melted microstructures which significantly impair the mechanical properties of the resultant scaffolds for potential applications in tissue engineering and regenerative medicine. Here, a novel post-treatment strategy was developed to endow the SLS-fabricated polycaprolactone (PCL) scaffolds with dense morphology and enhanced mechanical properties by embedding them in dense NaCl microparticles for in-situ re-melting and re-solidification. Read More

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http://dx.doi.org/10.1088/1758-5090/ab860eDOI Listing

Enhancing cell packing in buckyballs by acoustofluidic activation.

Biofabrication 2020 03 31;12(2):025033. Epub 2020 Mar 31.

Bio-Acoustic MEMS in Medicine (BAMM) Laboratory, Canary Center at Stanford for Cancer Early Detection, Department of Radiology, Stanford School of Medicine, Palo Alto, California 94304, United States of America.

How to pack materials into well-defined volumes efficiently has been a longstanding question of interest to physicists, material scientists, and mathematicians as these materials have broad applications ranging from shipping goods in commerce to seeds in agriculture and to spheroids in tissue engineering. How many marbles or gumball candies can you pack into a jar? Although these seem to be idle questions they have been studied for centuries and have recently become of greater interest with their broadening applications in science and medicine. Here, we study a similar problem where we try to pack cells into a spherical porous buckyball structure. Read More

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http://dx.doi.org/10.1088/1758-5090/ab76d9DOI Listing

Gravitational sedimentation-based approach for ultra-simple and flexible cell patterning coculture on microfluidic device.

Biofabrication 2020 Apr 29;12(3):035005. Epub 2020 Apr 29.

Department of Biomedical Engineering, City University of Hong Kong, Hong Kong, People's Republic of China.

Combining patterning coculture technique with microfluidics enables the reconstruction of complex in-vivo system to facilitate in-vitro studies on cell-cell and cell-environment interactions. However, simple and versatile approaches for patterning coculture of cells on microfluidic platforms remain lacking. In this study, a novel gravitational sedimentation-based approach is presented to achieve ultra-simple and flexible cell patterning coculture on a microfluidic platform, where multiple cell types can be patterned simultaneously to form a well-organized cell coculture. Read More

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http://dx.doi.org/10.1088/1758-5090/ab80b5DOI Listing

Crosslinker length dictates step-growth hydrogel network formation dynamics and allows rapid on-chip photoencapsulation.

Biofabrication 2020 Apr 22;12(3):035006. Epub 2020 Apr 22.

Department of Chemical Engineering, University of Wyoming, Laramie 82071, United States of America.

Hydrogels formed via free radical-mediated thiol-ene step-growth photopolymerization have been developed for a broad range of tissue engineering and regenerative medicine applications. While the crosslinking mechanism of thiol-ene hydrogels has been well-described, there has been only limited work exploring the physical differences among gels arising from variations in crosslinker properties. Here, we show that the character of linear polyethylene glycol (PEG) dithiols used to crosslink multi-arm polyethylene glycol norbornene (PEGNB) can be used as a facile strategy to tune hydrogel formation kinetics, and therefore the equilibrium hydrogel network architecture. Read More

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http://dx.doi.org/10.1088/1758-5090/ab7ef4DOI Listing

Hierarchical patterning via dynamic sacrificial printing of stimuli-responsive hydrogels.

Biofabrication 2020 Apr 22;12(3):035007. Epub 2020 Apr 22.

National Engineering Research Center for Tissue Restoration and Reconstruction (NERC-TRR), Guangzhou 510006, People's Republic of China. Department of Biomedical Engineering, School of Material Science and Engineering, South China University of Technology, Guangzhou 510006, People's Republic of China. Key Laboratory of Biomedical Engineering of Guangdong Province, South China University of Technology, Guangzhou 510641, People's Republic of China.

Inspired by stimuli-tailored dynamic processes that spatiotemporally create structural and functional diversity in biology, a new hierarchical patterning strategy is proposed to induce the emergence of complex multidimensional structures via dynamic sacrificial printing of stimuli-responsive hydrogels. Using thermally responsive gelatin (Gel) and pH-responsive chitosan (Chit) as proof-of-concept materials, we demonstrate that the initially printed sacrificial material (Gel/Chit-H hydrogel with a single gelatin network) can be converted dynamically into non-sacrificial material (Gel/Chit-H-Citr hydrogel with gelatin and an electrostatic citrate-chitosan dual network) under stimulus cues (citrate ions). Complex hierarchical structures and functions can be created by controlling either the printing patterns of citrate ink or the diffusion time of citrate ions into the Gel/Chit-H hydrogel. Read More

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http://dx.doi.org/10.1088/1758-5090/ab7e74DOI Listing

Directly coaxial 3D bioprinting of large-scale vascularized tissue constructs.

Biofabrication 2020 May 11;12(3):035014. Epub 2020 May 11.

State Key Laboratory of Fluid Power and Mechatronic Systems, School of Mechanical Engineering, Zhejiang University, Hangzhou 310027, People's Republic of China. Author to whom any correspondence should be addressed.

Three-dimensional (3D) bioprinting of soft large-scale tissues in vitro is still a big challenge due to two limitations, (i) the lack of an effective way to print fine nutrient delivery channels (NDCs) inside the cell-laden structures above the millimetre level; (ii) the need for a feasible strategy to vascularize NDCs. Here, a novel 3D bioprinting method is reported to directly print cell-laden structures with effectively vascularized NDCs. Bioinks with desired tissue cells and endothelial cells (ECs) are separately and simultaneously printed from the outside (mixed with GelMA) and inside (mixed with gelatin) of a coaxial nozzle. Read More

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http://dx.doi.org/10.1088/1758-5090/ab7e76DOI Listing

A novel waterborne polyurethane with biodegradability and high flexibility for 3D printing.

Biofabrication 2020 May 12;12(3):035015. Epub 2020 May 12.

School of Materials Science and Engineering, University of Science and Technology Beijing, Beijing 100083, People's Republic of China. These authors contributed equally to this work.

Three-dimensional (3D) printing provides a new approach of fabricating implantable products because it permits a flexible manner to extrude complex and customized shapes of the tissue scaffolds. Compared with other printable biomaterials, the polyurethane elastomer has several merits, including excellent mechanical properties and good biocompatibility. However, some intrinsic behavior, especially its high melting point and slow rate of degradation, hampered its application in 3D printed tissue engineering. Read More

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http://dx.doi.org/10.1088/1758-5090/ab7de0DOI Listing
May 2020
4.289 Impact Factor

Engineering of brain-like tissue constructs via 3D Cell-printing technology.

Biofabrication 2020 May 12;12(3):035016. Epub 2020 May 12.

Biomanufacturing Center, Department of Mechanical Engineering, Tsinghua University, Beijing 100084, People's Republic of China. Biomanufacturing and Rapid Forming Technology Key Laboratory of Beijing, Beijing 100084, People's Republic of China. 'Biomanufacturing and Engineering Living Systems' Innovation International Talents Base (111 Base), Beijing 100084, People's Republic of China.

The development of 3D Cell-printing technology contributes to the application of tissue constructs in vitro in neuroscience. Collecting neural cells from patients is an efficient way of monitoring health of an individual target, which, in turn, benefits the enhancement of medicines. The fabricated sample of neural cells is exposed to potential drugs for the analysis of neuron responses. Read More

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http://dx.doi.org/10.1088/1758-5090/ab7d76DOI Listing

Laser-assisted 3D bioprinting of exocrine pancreas spheroid models for cancer initiation study.

Biofabrication 2020 Apr 16;12(3):035001. Epub 2020 Apr 16.

Bioingénierie tissulaire, Université de Bordeaux, 146, rue Léo Saignat 33076, Bordeaux, France. Bioingénierie tissulaire, Inserm U1026, 146, rue Léo Saignat 33076, Bordeaux, France. Both authors have contributed equally to this work.

Pancreatic ductal adenocarcinoma (PDAC) is the most common malignancy of the pancreas. It has shown a poor prognosis and a rising incidence in the developed world. Other pathologies associated with this tissue include pancreatitis, a risk condition for pancreatic cancer. Read More

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http://dx.doi.org/10.1088/1758-5090/ab7cb8DOI Listing

Cryogenic 3D printing of heterogeneous scaffolds with gradient mechanical strengths and spatial delivery of osteogenic peptide/TGF-β1 for osteochondral tissue regeneration.

Biofabrication 2020 03 23;12(2):025030. Epub 2020 Mar 23.

College of Mechanical Engineering, Dongguan University of Technology, Dongguan, Guangdong, People's Republic of China. Contributed equally. Authors to whom any correspondence should be addressed.

Due to the increasing aging population and the high probability of sport injury among young people nowadays, it is of great demand to repair/regenerate diseased/defected osteochondral tissue. Given that osteochondral tissue mainly consists of a subchondral layer and a cartilage layer which are structurally heterogeneous and mechanically distinct, developing a biomimetic bi-phasic scaffold with excellent bonding strength to regenerate osteochondral tissue is highly desirable. Three-dimensional (3D) printing is advantageous in producing scaffolds with customized shape, designed structure/composition gradients and hence can be used to produce heterogeneous scaffolds for osteochondral tissue regeneration. Read More

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http://dx.doi.org/10.1088/1758-5090/ab7ab5DOI Listing

Drug compound screening in single and integrated multi-organoid body-on-a-chip systems.

Biofabrication 2020 02 26;12(2):025017. Epub 2020 Feb 26.

Wake Forest Institute for Regenerative Medicine, Wake Forest School of Medicine, Medical Center Boulevard, Winston-Salem, NC, 27101, United States of America. Department of Biomedical Engineering, The Ohio State University, 1080 Carmack Road, Columbus, OH, 43210, United States of America.

Current practices in drug development have led to therapeutic compounds being approved for widespread use in humans, only to be later withdrawn due to unanticipated toxicity. These occurrences are largely the result of erroneous data generated by in vivo and in vitro preclinical models that do not accurately recapitulate human physiology. Herein, a human primary cell- and stem cell-derived 3D organoid technology is employed to screen a panel of drugs that were recalled from market by the FDA. Read More

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http://dx.doi.org/10.1088/1758-5090/ab6d36DOI Listing
February 2020

The preparation of cell-containing microbubble scaffolds to mimic alveoli structure as a 3D drug-screening system for lung cancer.

Biofabrication 2020 03 27;12(2):025031. Epub 2020 Mar 27.

Institute of Biomedical Engineering, College of Medicine and College of Engineering, National Taiwan University, No. 49, Fanglan Rd, Taipei 10672, Taiwan.

Cancer is the leading cause of mortality worldwide, and lung cancer is the most malignant. However, the high failure rate in oncology drug development from in vitro studies to in vivo preclinical models indicates that the modern methods of evaluating drug efficacies in vitro are not reliable. Traditional 2D cell culture has proved inadequate to mimic real physiological conditions. Read More

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http://dx.doi.org/10.1088/1758-5090/ab78eeDOI Listing

Indirect selective laser sintering-printed microporous biphasic calcium phosphate scaffold promotes endogenous bone regeneration via activation of ERK1/2 signaling.

Biofabrication 2020 03 27;12(2):025032. Epub 2020 Mar 27.

The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory of Oral Biomedicine Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan, 430079, People's Republic of China.

The fabrication technique determines the physicochemical and biological properties of scaffolds, including the porosity, mechanical strength, osteoconductivity, and bone regenerative potential. Biphasic calcium phosphate (BCP)-based scaffolds are superior in bone tissue engineering due to their suitable physicochemical and biological properties. We developed an indirect selective laser sintering (SLS) printing strategy to fabricate 3D microporous BCP scaffolds for bone tissue engineering purposes. Read More

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http://dx.doi.org/10.1088/1758-5090/ab78edDOI Listing
March 2020
4.289 Impact Factor

A core-shell multi-drug platform to improve gastrointestinal tract microbial health using 3D printing.

Biofabrication 2020 03 13;12(2):025026. Epub 2020 Mar 13.

Department of Biomedical Engineering, Key Laboratory of Ministry of Education, Zhejiang University, Hangzhou 310027, People's Republic of China. Zhejiang Provincial Key Laboratory of Cardio-Cerebral Vascular Detection Technology and Medicinal Effectiveness Appraisal, Zhejiang University, Hangzhou 310027, People's Republic of China.

Improving the proliferation of probiotics (ca. Bifidobacterium) and inhibiting the growth of pathogenic bacteria (ca. Escherichia coli) is crucial for human health. Read More

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http://dx.doi.org/10.1088/1758-5090/ab782cDOI Listing

PLGA-collagen-ECM hybrid meshes mimicking stepwise osteogenesis and their influence on the osteogenic differentiation of hMSCs.

Biofabrication 2020 03 13;12(2):025027. Epub 2020 Mar 13.

Research Center for Functional Materials, National Institute for Materials Science, 1-1 Namiki, Tsukuba, Ibaraki, 305-0044, Japan. Department of Materials Science and Engineering, Graduate School of Pure and Applied Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, 305-8577, Japan.

Extracellular matrices (ECMs) are dynamically altered and remodeled during tissue development. How the dynamic remodeling of ECM affects stem cell functions remains poorly understood due to the difficulty of obtaining biomimetic ECMs. In this study, stepwise osteogenesis-mimicking ECM-deposited hybrid meshes were prepared by culturing human mesenchymal stem cells (hMSCs) in poly (lactic-co-glycolic acid) (PLGA)-collagen hybrid meshes and controlling the stages of the osteogenesis of hMSCs. Read More

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http://dx.doi.org/10.1088/1758-5090/ab782bDOI Listing

Nanocomposite bioink exploits dynamic covalent bonds between nanoparticles and polysaccharides for precision bioprinting.

Biofabrication 2020 03 13;12(2):025025. Epub 2020 Mar 13.

Tissue Engineering and Biofabrication Laboratory, Department of Health Sciences & Technology, ETH Zürich, Otto-Stern-Weg 7, 8093 Zürich, Switzerland.

The field of bioprinting has made significant recent progress towards engineering tissues with increasing complexity and functionality. It remains challenging, however, to develop bioinks with optimal biocompatibility and good printing fidelity. Here, we demonstrate enhanced printability of a polymer-based bioink based on dynamic covalent linkages between nanoparticles (NPs) and polymers, which retains good biocompatibility. Read More

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http://dx.doi.org/10.1088/1758-5090/ab782dDOI Listing

Entrapped in cage (EiC) scaffolds of 3D-printed polycaprolactone and porous silk fibroin for meniscus tissue engineering.

Biofabrication 2020 03 13;12(2):025028. Epub 2020 Mar 13.

3B's Research Group, I3Bs - Research Institute on Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, Parque de Ciência e Tecnologia, Zona Industrial da Gandra, 4805-017 Barco, Guimarães, Portugal. ICVS/3B's-PT Government Associate Laboratory, Braga/Guimarães, Portugal.

The meniscus has critical functions in the knee joint kinematics and homeostasis. Injuries of the meniscus are frequent, and the lack of a functional meniscus between the femur and tibial plateau can cause articular cartilage degeneration leading to osteoarthritis development and progression. Regeneration of meniscus tissue has outstanding challenges to be addressed. Read More

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http://dx.doi.org/10.1088/1758-5090/ab779fDOI Listing

A biofabricated vascularized skin model of atopic dermatitis for preclinical studies.

Biofabrication 2020 Apr 9;12(3):035002. Epub 2020 Apr 9.

National Center for Advancing Translational Sciences, National Institute of Health, Rockville, MD, United States of America.

Three-dimensional (3D) biofabrication techniques enable the production of multicellular tissue models as assay platforms for drug screening. The increased cellular and physiological complexity in these 3D tissue models should recapitulate the relevant biological environment found in the body. Here we describe the use of 3D bioprinting techniques to fabricate skin equivalent tissues of varying physiological complexity, including human epidermis, non-vascularized and vascularized full-thickness skin tissue equivalents, in a multi-well platform to enable drug screening. Read More

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http://dx.doi.org/10.1088/1758-5090/ab76a1DOI Listing

Microfabrication of poly(acrylamide) hydrogels with independently controlled topography and stiffness.

Biofabrication 2020 03 4;12(2):025023. Epub 2020 Mar 4.

Institute for Bioengineering of Catalonia (IBEC), The Barcelona Institute of Science and Technology (BIST), c/Baldiri Reixac 10-12, E-08028, Barcelona, Spain.

The stiffness and topography of a cell's extracellular matrix (ECM) are physical cues that play a key role in regulating processes that determine cellular fate and function. While substrate stiffness can dictate cell differentiation lineage, migration, and self-organization, topographical features can change the cell's differentiation profile or migration ability. Although both physical cues are present and intrinsic to the native tissues in vivo, in vitro studies have been hampered by the lack of technological set-ups that would be compatible with cell culture and characterization. Read More

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http://dx.doi.org/10.1088/1758-5090/ab7552DOI Listing

Scaffold-free and label-free biofabrication technology using levitational assembly in high magnetic field.

Biofabrication 2020 Feb 12. Epub 2020 Feb 12.

Department of Complex Tissue Regeneration (CTR), University of Maastricht , Universiteitssingel, 40, office 3.541A, Maastricht, 6229 ER, NETHERLANDS.

The feasibility of magnetic levitational bioassembly of tissue engineered constructs from living tissue spheroids in the presence of paramagnetic ions (i.e. Gd3+) was recently demonstrated. Read More

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http://dx.doi.org/10.1088/1758-5090/ab7554DOI Listing
February 2020

Engineering considerations on extrusion-based bioprinting: interactions of material behavior, mechanical forces and cells in the printing needle.

Biofabrication 2020 03 11;12(2):025022. Epub 2020 Mar 11.

Centre for Translational Bone, Joint and Soft Tissue Research, University Hospital Carl Gustav Carus, Faculty of Medicine, Technische Universität Dresden, Germany. Institute of Natural Materials Technology, Faculty of Mechanical Engineering, Technische Universität Dresden, Germany.

Systematic analysis of the extrusion process in 3D bioprinting is mandatory for process optimization concerning production speed, shape fidelity of the 3D construct and cell viability. In this study, we applied numerical and analytical modeling to describe the fluid flow inside the printing head based on a Herschel-Bulkley model. The presented analytical calculation method nicely reproduces the results of Computational Fluid Dynamics simulation concerning pressure drop over the printing head and maximal shear parameters at the outlet. Read More

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http://dx.doi.org/10.1088/1758-5090/ab7553DOI Listing

The bioprinting roadmap.

Biofabrication 2020 02 6;12(2):022002. Epub 2020 Feb 6.

Department of Mechanical Engineering and Mechanics, College of Engineering, Drexel University, 3141 Chestnut Street, Philadelphia, PA 19104, United States of America. Department of Mechanical Engineering, Tsinghua University, Beijing 100084, People's Republic of China.

This bioprinting roadmap features salient advances in selected applications of the technique and highlights the status of current developments and challenges, as well as envisioned advances in science and technology, to address the challenges to the young and evolving technique. The topics covered in this roadmap encompass the broad spectrum of bioprinting; from cell expansion and novel bioink development to cell/stem cell printing, from organoid-based tissue organization to bioprinting of human-scale tissue structures, and from building cell/tissue/organ-on-a-chip to biomanufacturing of multicellular engineered living systems. The emerging application of printing-in-space and an overview of bioprinting technologies are also included in this roadmap. Read More

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http://dx.doi.org/10.1088/1758-5090/ab5158DOI Listing
February 2020
4.289 Impact Factor

3D printable carboxylated cellulose nanocrystal-reinforced hydrogel inks for tissue engineering.

Biofabrication 2020 03 13;12(2):025029. Epub 2020 Mar 13.

To achieve a three-dimensional (3D) microenvironment for complex tissue regeneration is a great challenge when developing biomaterials as artificial extracellular matrix (ECM) with properties similar to that of native tissue. Polysaccharide-based hydrogel shows great potential as ECM in the regeneration of damaged tissues or reconstruction of organs, demonstrating properties similar to those of native ECM. Extrusion 3D printing of cell-free or cell-loaded hydrogel ink has led to a more sophisticated fabrication of the desired compositions and architectures for tissue engineering applications. Read More

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http://dx.doi.org/10.1088/1758-5090/ab736eDOI Listing
March 2020
4.289 Impact Factor

Handheld instrument for wound-conformal delivery of skin precursor sheets improves healing in full-thickness burns.

Biofabrication 2020 02 3;12(2):025002. Epub 2020 Feb 3.

Institute of Biomaterials and Biomedical Engineering, University of Toronto, 164 College Street, Toronto, Ontario M5S 3G9, Canada.

The current standard of care for patients with severe large-area burns consists of autologous skin grafting or acellular dermal substitutes. While emerging options to accelerate wound healing involve treatment with allogeneic or autologous cells, delivering cells to clinically relevant wound topologies, orientations, and sizes remains a challenge. Here, we report the one-step in situ formation of cell-containing biomaterial sheets using a handheld instrument that accommodates the topography of the wound. Read More

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http://dx.doi.org/10.1088/1758-5090/ab6413DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7042907PMC
February 2020

Nebulized jet-based printing of bio-electrical scaffolds for neural tissue engineering: a feasibility study.

Biofabrication 2020 03 4;12(2):025024. Epub 2020 Mar 4.

Department of Mechanical Engineering, Campus De Nayer, KU Leuven, Belgium. Department of Mechanical Engineering, University of Brescia, Brescia, Italy.

In this paper we investigate the application of a direct writing technique for printing conductive patterns onto a biocompatible electrospun-pyrolysed carbon-fibre-based substrate. The result is a first study towards the production of bio-electrical scaffolds that could be used to enhance the promotion of efficient connections among neurons for in vitro studies in the field of neural tissue engineering. An electrospinning process is employed for production of the materials derived from the precursor polyacrylonitrile, in which the embedding of carbon nanotubes (CNTs) is also investigated. Read More

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http://dx.doi.org/10.1088/1758-5090/ab71e0DOI Listing

Co-infection with Staphylococcus aureus after primary influenza virus infection leads to damage of the endothelium in a human alveolus-on-a-chip model.

Biofabrication 2020 02 19;12(2):025012. Epub 2020 Feb 19.

Institute of Medical Microbiology, Jena University Hospital, Am Klinikum 1, D-07747 Jena, Germany. Center for Sepsis Control and Care, Jena University Hospital, D-07747 Jena, Germany. Section of Experimental Virology, Institute of Medical Microbiology, Jena University Hospital, Hans-Knöll-Str. 2, D-07745, Jena, Germany.

Pneumonia is one of the most common infectious diseases worldwide. The influenza virus can cause severe epidemics, which results in significant morbidity and mortality. Beyond the virulence of the virus itself, epidemiological data suggest that bacterial co-infections are the major cause of increased mortality. Read More

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http://dx.doi.org/10.1088/1758-5090/ab7073DOI Listing
February 2020

Spherical microwell arrays for studying single cells and microtissues in 3D confinement.

Biofabrication 2020 02 26;12(2):025016. Epub 2020 Feb 26.

Institute of Physics, Academia Sinica, Taipei, Taiwan.

Microwell arrays have emerged as three-dimensional substrates for cell culture due to their simplicity of fabrication and promise for high-throughput applications such as 3D cell-based assays for drug screening. To date, most microwells have had cylindrical geometries. Motivated by our previous findings that cells display 3D physiological characteristics when grown in the spherical micropores of monodisperse foam scaffolds (Lee et al 2013 Integr. Read More

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http://dx.doi.org/10.1088/1758-5090/ab6edaDOI Listing
February 2020