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    522 results match your criteria Biofabrication[Journal]

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    Construction of a liver sinusoid based on the laminar flow on chip and self-assembly of endothelial cells.
    Biofabrication 2018 Feb 20;10(2):025010. Epub 2018 Feb 20.
    Graduate School at Shenzhen, Tsinghua University, Shenzhen, People's Republic of China. Open FIESTA Center, Tsinghua University, Shenzhen 518055, People's Republic of China. Department of Mechanical Engineering and Mechanics, Tsinghua University, Beijing, People's Republic of China.
    The liver is one of the main metabolic organs, and nearly all ingested drugs will be metabolized by the liver. Only a small fraction of drugs are able to come onto the market during drug development, and hepatic toxicity is a major cause for drug failure. Since drug development is costly in both time and materials, an in vitro liver model that can accelerate bioreactions in the liver and reduce drug consumption is imperative in the pharmaceutical industry. Read More

    Signal-amplifying nanoparticle/hydrogel hybrid microarray biosensor for metal-enhanced fluorescence detection of organophosphorus compounds.
    Biofabrication 2018 Feb 16. Epub 2018 Feb 16.
    Department of Chemical and Biomolecular Engineering, Yonsei University, Seoul, KOREA, REPUBLIC OF.
    In this study, we developed an enzyme-based miniaturized fluorescence biosensor to detect paraoxon, one of the most well-known neurotoxic organophosphorus compounds (OPs). The biosensor was fabricated with poly(ethylene glycol) (PEG) hydrogel microarrays that entrapped acetylcholinesterase (AChE) and quantum dots (QDs) as fluorescence reporters. Metal-enhanced fluorescence (MEF) was utilized to amplify the fluorescence signal, which was achieved by decorating QDs on the surface of silica-coated silver nanoparticles (Ag@Silica). Read More

    Sprouting angiogenesis in engineered pseudo islets.
    Biofabrication 2018 Feb 16. Epub 2018 Feb 16.
    Penn State, University Park, Pennsylvania, UNITED STATES.
    Despite the recent achievements in cell-based therapies for curing type-1 diabetes (T1D), capillarization in beta (β)-cell clusters is still a major roadblock as it is essential for long-term viability and function of β-cells in vivo. In this research, we report sprouting angiogenesis in engineered pseudo islets (EPIs) made of mouse insulinoma βTC3 cells and rat heart microvascular endothelial cells (RHMVECs). Upon culturing in three-dimensional (3D) constructs under angiogenic conditions, EPIs sprouted extensive capillaries into the surrounding matrix. Read More

    3D printed Lego®-like modular microfluidic devices based on capillary driving.
    Biofabrication 2018 Feb 8. Epub 2018 Feb 8.
    Department of Mechanical Engineering, Zhejiang University, Hangzhou, ZheJiang, 310027, CHINA.
    The field of how to rapidly assemble microfluidics with modular components continuously attracts researchers' attention, however extra efforts must be devoted to solving the problems of leaking and aligning between individual modules. This paper presents a novel type of modular microfluidic device, driven by capillary force. There is no necessity for a strict seal or special alignment, and its open structures make it easy to integrate various stents and reactants. Read More

    Microspheres containing decellularized cartilage induce chondrogenesis in vitro and remain functional after incorporation within a poly(caprolactone) filament useful for fabricating a 3D scaffold.
    Biofabrication 2018 Feb 2;10(2):025007. Epub 2018 Feb 2.
    Department of Orthopaedic Surgery, Cincinnati Children's Hospital Medical Center, Cincinnati, OH 45229, United States of America.
    In this study, articular cartilage was decellularized preserving a majority of the inherent proteins, cytokines, growth factors and sGAGs. The decellularized cartilage matrix (dCM) was then encapsulated in poly(lactic acid) microspheres (MS + dCM) via double emulsion. Blank microspheres without dCM, MS(-), were also produced. Read More

    Proof-of-concept: 3D bioprinting of pigmented human skin constructs.
    Biofabrication 2018 Jan 23;10(2):025005. Epub 2018 Jan 23.
    Bio-Manufacturing Programme, Singapore Institute of Manufacturing Technology (SIMTech), Agency for Science, Technology and Research (A*STAR), 71 Nanyang Drive, 638075, Singapore. Singapore Centre for 3D Printing (SC3DP), School of Mechanical and Aerospace Engineering, Nanyang Technological University (NTU), 50 Nanyang Avenue, 639798, Singapore.
    Three-dimensional (3D) pigmented human skin constructs have been fabricated using a 3D bioprinting approach. The 3D pigmented human skin constructs are obtained from using three different types of skin cells (keratinocytes, melanocytes and fibroblasts from three different skin donors) and they exhibit similar constitutive pigmentation (pale pigmentation) as the skin donors. A two-step drop-on-demand bioprinting strategy facilitates the deposition of cell droplets to emulate the epidermal melanin units (pre-defined patterning of keratinocytes and melanocytes at the desired positions) and manipulation of the microenvironment to fabricate 3D biomimetic hierarchical porous structures found in native skin tissue. Read More

    Leaf-templated, microwell-integrated microfluidic chips for high-throughput cell experiments.
    Biofabrication 2018 Jan 19. Epub 2018 Jan 19.
    Department of Mechanical Engineering, Xi'an Jiaotong University, Xi'an, CHINA.
    As an alternative to conventional cell culture and animal testing, organ-on-a-chip is applied to study the biological phenomena of organ development and disease, as well as the interactions between human tissues and external stimuli such as chemicals, forces and electricity. The pattern design of microfluidic channel is one of the key approaches to regulate cell growth and differentiation, because these channels work as a crucial vasculature system to control the fluidic flow throughout the organ-on-a-chip device. In this study, we introduce a novel leaf-templated, microwell-integrated microfluidic chip for high-throughput cell experiments, consisting of a leaf-venation layer for fluent fluid flow, and a microwell-array layer for cell to reside. Read More

    Automated fabrication of photopatterned gelatin hydrogels for organ-on-chips applications.
    Biofabrication 2018 Jan 16;10(2):025004. Epub 2018 Jan 16.
    Disease Biophysics Group, Wyss Institute for Biologically Inspired Engineering, John A. Paulson School of Engineering and Applied Sciences, Harvard University, Cambridge, MA, United States of America.
    Organ-on-chip platforms aim to improve preclinical models for organ-level responses to novel drug compounds. Heart-on-a-chip assays in particular require tissue engineering techniques that rely on labor-intensive photolithographic fabrication or resolution-limited 3D printing of micropatterned substrates, which limits turnover and flexibility of prototyping. We present a rapid and automated method for large scale on-demand micropatterning of gelatin hydrogels for organ-on-chip applications using a novel biocompatible laser-etching approach. Read More

    Laser-direct writing by two-photon polymerization of 3D honeycomb-like structures for bone regeneration.
    Biofabrication 2018 Jan 12. Epub 2018 Jan 12.
    Laser Department, National Institute for Laser Plasma and Radiation Physics, PO Box MG-36, Magurele, Magurele, ROMANIA.
    A major limitation of existing 3D implantable structures for bone tissue engineering is that most of the cells rapidly attach on the outer edges of the structure, restricting the cells penetration into the inner parts and causing the formation of a necrotic core. Furthermore, these structures generally possess a random spatial arrangement and do not preserve the isotropy on the whole volume. Here, we report on the fabrication and testing of an innovative 3D hierarchical, honeycomb-like structure (HS), with reproducible and isotropic arhitecture, that allows "in volume" migration of osteoblasts. Read More

    A dentin-derived hydrogel bioink for 3D bioprinting of cell laden scaffolds for regenerative dentistry.
    Biofabrication 2018 Jan 10;10(2):024101. Epub 2018 Jan 10.
    Division of Biomaterials and Biomechanics, Department of Restorative Dentistry, OHSU School of Dentistry, Portland, OR, United States of America.
    Recent studies in tissue engineering have adopted extracellular matrix (ECM) derived scaffolds as natural and cytocompatible microenvironments for tissue regeneration. The dentin matrix, specifically, has been shown to be associated with a host of soluble and insoluble signaling molecules that can promote odontogenesis. Here, we have developed a novel bioink, blending printable alginate (3% w/v) hydrogels with the soluble and insoluble fractions of the dentin matrix. Read More

    Scalable and physiologically relevant microenvironments for human pluripotent stem cell expansion and differentiation.
    Biofabrication 2018 Jan 10. Epub 2018 Jan 10.
    Department of Chemical and Biomolecular Engineering, University of Nebraska, Lincoln, Nebraska, USA, 820 N 16th st, Othmer 207, lincoln, Nebraska, UNITED STATES.
    Human pluripotent stem cells (hPSCs) are required in large numbers for various biomedical applications. However, the scalable and cost-effective culturing of high quality hPSCs and their derivatives remains very challenging. Here, we report a novel and physiologically relevant 3D culture system (called the AlgTube cell culture system) for hPSC expansion and differentiation. Read More

    Microspheres containing decellularized cartilage induce chondrogenesis in vitro and remain functional after incorporation within a poly(caprolactone) filament useful for fabricating a 3D scaffold.
    Biofabrication 2018 Jan 9. Epub 2018 Jan 9.
    Department of Orthopaedic Surgery, Cincinnati Children`s Hospital Medical Center, Cincinnati, Cincinnati, Ohio, UNITED STATES.
    In this study, articular cartilage was decellularized preserving a majority of the inherent proteins, cytokines, growth factors and sGAGs. The decellularized cartilage matrix (dCM) was then encapsulated in poly(lactic acid) microspheres (MS+dCM) via double emulsion. Blank microspheres without dCM, MS(-), were also produced. Read More

    Direct three-dimensional printing of polymeric scaffolds with nanofibrous topography.
    Biofabrication 2017 Dec 13. Epub 2017 Dec 13.
    Division of Regenerative Medicine and Cellular Therapies, University of Nottingham School of Pharmacy, Nottingham, Nottingham, UNITED KINGDOM OF GREAT BRITAIN AND NORTHERN IRELAND.
    Three-dimensional (3D) printing is a powerful manufacturing tool for making 3D structures with well-defined architectures for a wide range of applications. The field of tissue engineering has also adopted this technology to fabricate scaffolds for tissue regeneration. The ability to control architecture of scaffolds, e. Read More

    Contact guidance for cardiac tissue engineering using 3D bioprinted gelatin patterned hydrogel.
    Biofabrication 2017 Dec 13. Epub 2017 Dec 13.
    School of Materials Science and Engineering, Nanyang Technological University, Office: N4.1-02-06, College of Engineering , Block N4.1, 50 Nanyang Avenue, SINGAPORE 639798, Singapore, SINGAPORE.
    Here, we have developed a 3D bioprinted microchanneled gelatin hydrogel that promotes human mesenchymal stem cell (hMSC) myocardial commitment and supports native cardiomyocytes (CMs) contractile functionality. Firstly, we studied the effect of bioprinted microchanneled hydrogel on the alignment, elongation, and differentiation of hMSC. Notably, the cells displayed well defined F-actin anisotropy and elongated morphology on the microchanneled hydrogel, hence showing the effects of topographical control over cell behavior. Read More

    Automated 3D bioassembly of micro-tissues for biofabrication of hybrid tissue engineered constructs.
    Biofabrication 2017 Dec 4. Epub 2017 Dec 4.
    Christchurch Regenerative Medicine and Tissue Engineering (CReaTE) Group, Department of Orthopaedic Surgery, Centre for Bioengineering & Nanomedicine, University of Otago Christchurch, Christchurch, NEW ZEALAND.
    Bottom-up biofabrication approaches combining micro-tissue fabrication techniques with extrusion-based 3D printing of thermoplastic polymer scaffolds are emerging strategies in tissue engineering. These biofabrication strategies support native self-assembly mechanisms observed in developmental stages of tissue or organoid growth as well as promoting cell-cell interactions and cell differentiation capacity. Few technologies have been developed to automate the precise assembly of micro-tissues or tissue modules into structural scaffolds. Read More

    Networked concave microwell arrays for constructing 3D cell spheroids.
    Biofabrication 2017 Nov 30;10(1):015001. Epub 2017 Nov 30.
    KU-KIST Graduate School of Converging Science and Technology, Korea University, Seoul 02841, Republic of Korea.
    The engineered three-dimensional (3D) cell cultivation system for the production of multicellular spheroids has attracted considerable attention due to its improved in vivo relevance to cellular communications compared with the traditional two-dimensional (2D) cell culture platform. The formation and maintenance of cell spheroids in a healthy condition is the critical factor for tissue engineering applications such as the repair of damaged tissues, the development of organ replacement parts and preclinical drug tests. However, culturing spheroids in conventional isolated single wells shows limited yield and reduced maintenance periods due to the lack of proper supplies of nutrition as well as intercellular chemical signaling. Read More

    One-step delivery of a functional multi-layered cell sheet using a thermally expandable hydrogel with controlled presentation of cell adhesive proteins.
    Biofabrication 2017 Nov 27. Epub 2017 Nov 27.
    Department of Bioengineering, Hanyang University, 222 Wangsimni-ro, Seongdong-gu, Seoul, 04763, KOREA, REPUBLIC OF.
    In this study, we developed a new system enabling rapid delivery of a multi-layered cell sheet by combining layer-by-layer (LBL) coating of a cell membrane and surface engineered thermally expandable hydrogel. Human dermal fibroblasts were LBL-coated with fibronectin (FN) and gelatin to form a multi-layered cell sheet in a single seeding step via spontaneous 3D cell-cell interactions. FN was covalently immobilized onto the surface of a Tetronic®-based hydrogel at two different concentrations (1 and 5 g/ml) for stable adhesion of the multi-layered cell sheet, followed by polydopamine (PD) coating. Read More

    Coaxial extrusion bioprinting of 3D microfibrous constructs with cell-favorable gelatin methacryloyl microenvironments.
    Biofabrication 2017 Nov 27. Epub 2017 Nov 27.
    Harvard Medical School, Cambridge, UNITED STATES.
    Bioinks with shear-thinning/rapid solidification properties and strong mechanics are usually needed for the bioprinting of three-dimensional (3D) cell-laden constructs. As such, it remains challenging to generate soft constructs from bioinks at low concentrations that are favorable for cellular activities. Herein, we report a strategy to fabricate cell-laden constructs with tunable 3D microenvironments achieved by bioprinting of gelatin methacryloyl (GelMA)/alginate core/sheath microfibers, where the alginate sheath serves as a template to support and confine the GelMA pre-hydrogel in the core during the extrusion process, allowing for subsequent UV crosslinking. Read More

    PLA short sub-micron fiber reinforcement of 3D bioprinted alginate constructs for cartilage regeneration.
    Biofabrication 2017 Nov 14;9(4):044105. Epub 2017 Nov 14.
    Warsaw University of Technology, Faculty of Materials Science and Engineering, 02-507 Warsaw, Poland.
    In this study, we present an innovative strategy to reinforce 3D-printed hydrogel constructs for cartilage tissue engineering by formulating composite bioinks containing alginate and short sub-micron polylactide (PLA) fibers. We demonstrate that Young's modulus obtained for pristine alginate constructs (6.9 ± 1. Read More

    Effect of scaffold morphology and cell co-culture on tenogenic differentiation of HADMSC on centrifugal melt electrospun poly (L‑lactic acid) fibrous meshes.
    Biofabrication 2017 Nov 14;9(4):044106. Epub 2017 Nov 14.
    Mary & Dick Holland Regenerative Medicine Program, University of Nebraska Medical Center, Omaha, NE, United States of America. Division of Cardiology, Department of Internal Medicine, University of Nebraska Medical Center, Omaha, NE, United States of America.
    Engineered tendon grafts offer a promising alternative for grafting during the reconstruction of complex tendon tears. The tissue-engineered tendon substitutes have the advantage of increased biosafety and the option to customize their biochemical and biophysical properties to promote tendon regeneration. In this study, we developed a novel centrifugal melt electrospinning (CME) technique, with the goal of optimizing the fabrication parameters to generate fibrous scaffolds for tendon tissue engineering. Read More

    Networked concave microwell arrays for constructing 3-D cell spheroids.
    Biofabrication 2017 Nov 6. Epub 2017 Nov 6.
    KU-KIST Graduate School of Converging Science and Technology, Korea University, 145 Anam-ro, Seongbuk-gu, R&D Center #410, Seoul, 02841, Korea (the Republic of).
    The engineered three-dimensional (3-D) cell cultivation system for the production of multicellular spheroids has attracted considerable attention due to its improved in vivo relevance to cellular communications compared to the traditional two-dimensional (2-D) cell culture platform. The formation and maintenance of cell spheroids in healthy condition is the critical factor for tissue engineering applications such as the repair of damaged tissues, the development of organ replacement parts, and preclinical drug tests. However, culturing spheroids in conventional isolated single wells show limit ted yield and maintenance periods due to the lack of proper supplies of nutrition as well as intercellular chemical signaling. Read More

    Bioprinted chitosan-gelatin thermosensitive hydrogels using an inexpensive 3D printer.
    Biofabrication 2017 Nov 30;10(1):015002. Epub 2017 Nov 30.
    School of Chemical Engineering, Oklahoma State University, 420 Engineering North, Stillwater, OK 74078, United States of America.
    The primary bottleneck in bioprinting cell-laden structures with carefully controlled spatial relation is a lack of biocompatible inks and printing conditions. In this regard, we explored using thermogelling chitosan-gelatin (CG) hydrogel as a novel bioprinting ink; CG hydrogels are unique in that it undergoes a spontaneous phase change at physiological temperature, and does not need post-processing. In addition, we used a low cost (<$800) compact 3D printer, and modified with a new extruder to print using disposable syringes and hypodermic needles. Read More

    Optimization of binding B-lymphocytes in a microfluidic channel: surface modification, stasis time and shear response.
    Biofabrication 2017 Nov 30;10(1):014101. Epub 2017 Nov 30.
    Future Industries Institute, University of South Australia, Mawson Lakes Campus, Mawson Lakes Boulevard, Mawson Lakes, SA 5095, Australia.
    Binding and maintaining cells inside microfluidic channels is a challenging task due to the potential release of cells from the channels with the flow and accompanying shear stress. In this work we optimized the binding of human B-lymphocyte cells (HR1K) inside a microfluidic channel and determined the strength of this binding under shear stress of flowing liquid. In order to determine the parameters required for a live/dead test in microfluidic devices, populations of both living and dead cells were tested separately. Read More

    Hypergravity-induced multicellular spheroid generation with different morphological patterns precisely controlled on a centrifugal microfluidic platform.
    Biofabrication 2017 Nov 14;9(4):045006. Epub 2017 Nov 14.
    Interdisciplinary Program for Bioengineering, Seoul National University Graduate School, Seoul 03087, Republic of Korea.
    In living tissue, cells exist in three-dimensional (3D) microenvironments with intricate cell-cell interactions. To model these cellular environments, numerous techniques for generating cell spheroids have been proposed and improved. However, previously reported methods still have limitations in uniformity, reproducibility, scalability, throughput, etc. Read More

    Facile and controllable electrochemical fabrication of cell-adhesive polypyrrole electrodes using pyrrole-RGD peptides.
    Biofabrication 2017 Nov 14;9(4):045007. Epub 2017 Nov 14.
    School of Materials Science and Engineering, Gwangju Institute of Science and Technology, Gwangju 61005, Republic of Korea.
    Electrically conductive polymers, such as polypyrrole (PPy), have been widely used for the fabrication of various biosensors and tissue engineering scaffolds. For their biologically relevant applications, conductive biomaterials capable of intimate cellular interactions are highly desired. However, conventional methods to incorporate biomolecules into conductive polymers do not offer fine and easy control over the surface density of the biomolecules and/or their stability. Read More

    Bone matrix production in hydroxyapatite-modified hydrogels suitable for bone bioprinting.
    Biofabrication 2017 Nov 14;9(4):044103. Epub 2017 Nov 14.
    Institute of Interfacial Process Engineering and Plasma Technology, University of Stuttgart, Nobelstraße 12, D-70569 Stuttgart, Germany.
    Though bioprinting is a forward-looking approach in bone tissue engineering, the development of bioinks which are on the one hand processable with the chosen printing technique, and on the other hand possess the relevant mechanical as well as osteoconductive features remains a challenge. In the present study, polymer solutions based on methacrylated gelatin and methacrylated hyaluronic acid modified with hydroxyapatite (HAp) particles (5 wt%) were prepared. Encapsulation of primary human adipose-derived stem cells in the HAp-containing gels and culture for 28 d resulted in a storage moduli significantly increased to 126% ± 9. Read More

    Recombinant spider silk-based bioinks.
    Biofabrication 2017 Nov 14;9(4):044104. Epub 2017 Nov 14.
    Lehrstuhl Biomaterialien, Bayreuther Zentrum für Kolloide und Grenzflächen (BZKG), Bayreuther Zentrum für Bio-Makromoleküle (bio-mac), Bayreuther Zentrum für Molekulare Biowissenschaften (BZMB), Bayreuther Materialzentrum (BayMAT), Bayerisches Polymerinstitut (BPI) Universitätsstraße 30, Universität Bayreuth, Bayreuth D-95447, Germany.
    Bioinks, 3D cell culture systems which can be printed, are still in the early development stages. Currently, extensive research is going into designing printers to be more accommodating to bioinks, designing scaffolds with stiff materials as support structures for the often soft bioinks, and modifying the bioinks themselves. Recombinant spider silk proteins, a potential biomaterial component for bioinks, have high biocompatibility, can be processed into several morphologies and can be modified with cell adhesion motifs to enhance their bioactivity. Read More

    Assessing bioink shape fidelity to aid material development in 3D bioprinting.
    Biofabrication 2017 Nov 30;10(1):014102. Epub 2017 Nov 30.
    Department of Orthopaedics, University Medical Center Utrecht, Utrecht, The Netherlands.
    During extrusion-based bioprinting, the deposited bioink filaments are subjected to deformations, such as collapse of overhanging filaments, which compromises the ability to stack several layers of bioink, and fusion between adjacent filaments, which compromises the resolution and maintenance of a desired pore structure. When developing new bioinks, approaches to assess their shape fidelity after printing would be beneficial to evaluate the degree of deformation of the deposited filament and to estimate how similar the final printed construct would be to the design. However, shape fidelity has been prevalently assessed qualitatively through visual inspection after printing, hampering the direct comparison of the printability of different bioinks. Read More

    Automated fabrication of hydrogel microfibers with tunable diameters for controlled cell alignment.
    Biofabrication 2017 Nov 14;9(4):045009. Epub 2017 Nov 14.
    National Engineering Research Center for Biomaterials, Sichuan University, Sichuan, Chengdu 610064, People's Republic of China.
    A newly designed spinning device was utilized to produce continuous hydrogel microfibers with tunable diameters. It was found that the diameter of the microfiber was dependent on perfusion speed and coagulation wheel rotation rate. Their correlation was finally described by a mathematical expression, which proved to be useful for a size-tunable spinning technique. Read More

    Creating hierarchical porosity hydroxyapatite scaffolds with osteoinduction by three-dimensional printing and microwave sintering.
    Biofabrication 2017 Nov 14;9(4):045008. Epub 2017 Nov 14.
    National Engineering Research Center for Biomaterials, Sichuan University, Chengdu, 610064, People's Republic of China.
    Hierarchical porosity, which includes micropores and macropores in scaffolds, contributes to important multiple biological functions for tissue regeneration. This paper introduces a two-step method of combining three-dimensional printing (3DP) and microwave sintering to fabricate two-level hierarchical porous scaffolds. The results showed that 3D printing made the macroporous structure well-controlled and microwave sintering generated micropores on the macropore surface. Read More

    PLA short sub-micron fibers reinforcement of 3D bioprinted alginate constructs for cartilage regeneration.
    Biofabrication 2017 Oct 4. Epub 2017 Oct 4.
    Faculty of Materials Science and Engineering, 02507, Warsaw University of Technology, Warsaw, POLAND.
    In this study, we present an innovative strategy to reinforce 3D printed hydrogel constructs for cartilage tissue engineering by formulating composite bioinks containing alginate and short sub-micron polylactide (PLA) fibers. We demonstrate that Young's modulus obtained for pristine alginate constructs (6.9 ± 1. Read More

    Proposal to assess printability of bioinks for extrusion-based bioprinting and evaluation of rheological properties governing bioprintability.
    Biofabrication 2017 Nov 14;9(4):044107. Epub 2017 Nov 14.
    Department for Functional Materials in Medicine and Dentistry and Bavarian Polymer Institute, Julius-Maximilians-Universität Würzburg, Germany. Institute of Health and Biomedical Innovation, Queensland University of Technology (QUT), Brisbane, Australia.
    The development and formulation of printable inks for extrusion-based 3D bioprinting has been a major challenge in the field of biofabrication. Inks, often polymer solutions with the addition of crosslinking to form hydrogels, must not only display adequate mechanical properties for the chosen application but also show high biocompatibility as well as printability. Here we describe a reproducible two-step method for the assessment of the printability of inks for bioprinting, focussing firstly on screening ink formulations to assess fibre formation and the ability to form 3D constructs before presenting a method for the rheological evaluation of inks to characterise the yield point, shear thinning and recovery behaviour. Read More

    Biomimetic matrix fabricated by LMP-1 gene-transduced MC3T3-E1 cells for bone regeneration.
    Biofabrication 2017 Nov 14;9(4):045010. Epub 2017 Nov 14.
    Department of Orthopedic, Peking University Shenzhen Hospital, Shenzhen, People's Republic of China. Guangdong Provincial Key Laboratory of Orthopedics and Traumatology, The First Affiliate Hospital of Sun Yat-sen University, Guangzhou, People's Republic of China.
    Bone healing is regulated by multiple microenvironmental signals provided by the extracellular matrix (ECM). This study aimed to mimic the native osteoinductive microenvironment by developing an ECM using gene-transduced cells. The LIM mineralization protein-1 (LMP-1) gene was transferred to murine pre-osteoblast cells (MC3T3-E1) using lentiviral vectors. Read More

    Double printing of hyaluronic acid/poly(glycidol) hybrid hydrogels with poly(ε-caprolactone) for MSC chondrogenesis.
    Biofabrication 2017 Nov 14;9(4):044108. Epub 2017 Nov 14.
    Department of Functional Materials in Medicine and Dentistry and Bavarian Polymer Institute, University of Würzburg, Pleicherwall 2, 97070 Würzburg, Germany.
    This study investigates the use of allyl-functionalized poly(glycidol)s (P(AGE-co-G)) as a cytocompatible cross-linker for thiol-functionalized hyaluronic acid (HA-SH) and the optimization of this hybrid hydrogel as bioink for 3D bioprinting. The chemical cross-linking of gels with 10 wt.% overall polymer concentration was achieved by a UV-induced radical thiol-ene coupling between the thiol and allyl groups. Read More

    A 3D printed microfluidic perfusion device for multicellular spheroid cultures.
    Biofabrication 2017 Sep 11;9(4):045005. Epub 2017 Sep 11.
    Department of Biomedical Engineering, National University of Singapore, 4, Engineering Drive 3, E4-04-10, Singapore 117583, Singapore.
    The advent of 3D printing technologies promises to make microfluidic organ-on-chip technologies more accessible for the biological research community. To date, hydrogel-encapsulated cells have been successfully incorporated into 3D printed microfluidic devices. However, there is currently no 3D printed microfluidic device that can support multicellular spheroid culture, which facilitates extensive cell-cell contacts important for recapitulating many multicellular functional biological structures. Read More

    Laser processing of protein films as a method for accomplishment of cell patterning at the microscale.
    Biofabrication 2017 Sep 7;9(4):045004. Epub 2017 Sep 7.
    Department of Materials Science and Technology, University of Crete, Vassilika Vouton, 710 03 Heraklion, Crete, Greece. Institute of Electronic Structure and Laser (IESL), Foundation for Research and Technology Hellas (FORTH), PO Box 527, Vassilika Vouton, 711 10 Heraklion, Crete, Greece.
    In this study, we propose a photostructuring approach for protein films based on a treatment with nanosecond pulses of a KrF excimer laser. As a model protein we used an amyloid fibril-forming protein. Laser treatment induced a foaming of the sample surface exhibiting an interconnected fibrous mesh with a high degree of control and precision. Read More

    Green bioprinting: extrusion-based fabrication of plant cell-laden biopolymer hydrogel scaffolds.
    Biofabrication 2017 Nov 14;9(4):045011. Epub 2017 Nov 14.
    Centre for Translational Bone, Joint and Soft Tissue Research, University Hospital Carl Gustav Carus and Faculty of Medicine of Technische Universität Dresden, Germany. Institute of Natural Materials Technology, Faculty of Mechanical Engineering of Technische Universität Dresden, German y.
    Plant cell cultures produce active agents for pharmaceuticals, food and cosmetics. However, up to now process control for plant cell suspension cultures is challenging. A positive impact of cell immobilization, such as encapsulation in hydrogel beads, on secondary metabolites production has been reported for several plant species. Read More

    Engineering the mechanical and biological properties of nanofibrous vascular grafts for in situ vascular tissue engineering.
    Biofabrication 2017 Aug 17;9(3):035007. Epub 2017 Aug 17.
    Department of Bioengineering, University of California, Berkeley, CA 94720, United States of America. UC Berkeley and UCSF Bioengineering Graduate Program, Berkeley, CA94720, United States of America.
    Synthetic small diameter vascular grafts have a high failure rate, and endothelialization is critical for preventing thrombosis and graft occlusion. A promising approach is in situ tissue engineering, whereby an acellular scaffold is implanted and provides stimulatory cues to guide the in situ remodeling into a functional blood vessel. An ideal scaffold should have sufficient binding sites for biomolecule immobilization and a mechanical property similar to native tissue. Read More

    A microfluidic platform for modeling metastatic cancer cell matrix invasion.
    Biofabrication 2017 Sep 1;9(4):045001. Epub 2017 Sep 1.
    Department of Biomedical Engineering, Boston University, Boston, MA 02215, United States of America.
    Invasion of the extracellular matrix is a critical step in the colonization of metastatic tumors. The invasion process is thought to be driven by both chemokine signaling and interactions between invading cancer cells and physical components of the metastatic niche, including endothelial cells that line capillary walls and serve as a barrier to both diffusion and invasion of the underlying tissue. Transwell chambers, a tool for generating artificial chemokine gradients to induce cell migration, have facilitated recent work to investigate the chemokine contributions to matrix invasion. Read More

    Quantitative criteria to benchmark new and existing bio-inks for cell compatibility.
    Biofabrication 2017 Sep 1;9(4):044102. Epub 2017 Sep 1.
    Recent advancements in 3D bioprinting have led to the fabrication of more complex, more precise, and larger printed tissue constructs. As the field continues to advance, it is critical to develop quantitative benchmarks to compare different bio-inks for key cell-biomaterial interactions, including (1) cell sedimentation within the ink cartridge, (2) cell viability during extrusion, and (3) cell viability after ink curing. Here we develop three simple protocols for quantitative analysis of bio-ink performance. Read More

    GelMA-collagen blends enable drop-on-demand 3D printablility and promote angiogenesis.
    Biofabrication 2017 Sep 1;9(4):045002. Epub 2017 Sep 1.
    Department of Dental Materials and Biomaterials Research, RWTH Aachen University Hospital, Aachen, Germany.
    Effective vascularization is crucial for three-dimensional (3D) printed hydrogel-cell constructs to efficiently supply cells with oxygen and nutrients. Till date, several hydrogel blends have been developed that allow the in vitro formation of a capillary-like network within the gels but comparatively less effort has been made to improve the suitability of the materials for a 3D bioprinting process. Therefore, we hypothesize that tailored hydrogel blends of photo-crosslinkable gelatin and type I collagen exhibit favorable 3D drop-on-demand printing characteristics in terms of rheological and mechanical properties and that further capillary-like network formation can be induced by co-culturing human umbilical vein endothelial cells and human mesenchymal stem cells within the proposed blends. Read More

    Fabrication of arbitrary 3D components in cardiac surgery: from macro-, micro- to nanoscale.
    Biofabrication 2017 Aug 3;9(3):032002. Epub 2017 Aug 3.
    Institute of Biomaterials and Tissue Engineering, Huaqiao University, Xiamen 361021, People's Republic of China. Fujian Provincial Key Laboratory of Biochemical Technology, Xiamen 361021, People's Republic of China.
    Fabrication of tissue-/organ-like structures at arbitrary geometries by mimicking the properties of the complex material offers enormous interest to the research and clinical applicability in cardiovascular diseases. Patient-specific, durable, and realistic three-dimensional (3D) cardiac models for anatomic consideration have been developed for education, pro-surgery planning, and intra-surgery guidance. In cardiac tissue engineering (TE), 3D printing technology is the most convenient and efficient microfabrication method to create biomimetic cardiovascular tissue for the potential in vivo implantation. Read More

    Comparative study of gelatin methacrylate hydrogels from different sources for biofabrication applications.
    Biofabrication 2017 Aug 21;9(4):044101. Epub 2017 Aug 21.
    School of Engineering, Faculty of Applied Sciences, University of British Columbia, Kelowna, BC, V1V 1V7, Canada.
    Gelatin methacrylate (GelMA) hydrogel is a promising bioink for biofabrication applications due to its cost-effectiveness, ease of synthesis and biocompatibility to allow cell adhesion. However, the GelMA synthesized from a widely used porcine skin gelatin has a thermal gelation problem at room temperature. Here, we present thermally stable GelMA hydrogels at room temperature while maintaining the mechanical and biological properties comparable to porcine GelMA. Read More

    Engineering-derived approaches for iPSC preparation, expansion, differentiation and applications.
    Biofabrication 2017 Jul 31;9(3):032001. Epub 2017 Jul 31.
    Department of Mechanical Engineering, 111 'Biomanufacturing and Engineering Living Systems' Innovation International Talents Base, Tsinghua University, Beijing, People's Republic of China.
    Remarkable achievements have been made since induced pluripotent stem cells (iPSCs) were first introduced in 2006. Compared with non-pluripotent stem cells, iPSC research faces several additional complexities, such as the choice of extracellular matrix proteins, growth and differentiation factors, as well as technical challenges related to self-renewal and directed differentiation. Overcoming these challenges requires the integration of knowledge and technologies from multiple fields including cell biology, biomaterial science, engineering, physics and medicine. Read More

    DC biased low-frequency insulating constriction dielectrophoresis for protein biomolecules concentration.
    Biofabrication 2017 Sep 1;9(4):045003. Epub 2017 Sep 1.
    Lane Department of Computer Science and Electrical Engineering, West Virginia University, Morgantown, West Virginia 26506, United States of America.
    Sample enrichment or molecules concentration is considered an essential step in sample processing of miniaturized devices aimed at biosensing and bioanalysis. Among all the means involved to achieve this aim, dielectrophoresis (DEP) is increasingly employed in molecules manipulation and concentration because it is non-destructive and high efficiency. This paper presents a methodology to achieve protein concentration utilizing the combination effects of electrokinetics and low frequency insulating dielectrophoresis (iDEP) generated within a microfluidic device, in which a submicron constricted channel was fabricated using DNA molecular combing and replica molding. Read More

    A novel cylindrical microwell featuring inverted-pyramidal opening for efficient cell spheroid formation without cell loss.
    Biofabrication 2017 Aug 14;9(3):035006. Epub 2017 Aug 14.
    Medical Device Research Center, Research Institute for Future Medicine, Samsung Medical Center, Seoul 06351, Republic of Korea.
    Spheroid cultures have been often used to simulate and understand in situ biological occurrences with potential to be further applied to therapeutic approaches, such as cell transplantation. However, traditional lab-scale techniques hardly reached the needed large scale production of cell spheroids, thus limiting their versatility in many biomedical fields. Microscale technologies have rapidly improved in the last decade, and contributed to the large scale production of cell spheroids with high controllability and reproducibility. Read More

    Towards 4D printed scaffolds for tissue engineering: exploiting 3D shape memory polymers to deliver time-controlled stimulus on cultured cells.
    Biofabrication 2017 Aug 2;9(3):031001. Epub 2017 Aug 2.
    University of Twente, MIRA Institute for Biomedical Technology and Technical Medicine, Department of Tissue Regeneration, 7500AE, Enschede, The Netherlands.
    Tissue engineering needs innovative solutions to better fit the requirements of a minimally invasive approach, providing at the same time instructive cues to cells. The use of shape memory polyurethane has been investigated by producing 4D scaffolds via additive manufacturing technology. Scaffolds with two different pore network configurations (0/90° and 0/45°) were characterized by dynamic-mechanical analysis. Read More

    Rising to the challenge: applying biofabrication approaches for better drug and chemical product development.
    Biofabrication 2017 Jul 19;9(3):033001. Epub 2017 Jul 19.
    National Centre for the Replacement, Refinement and Reduction of Animals in Research, London NW1 2BE, United Kingdom.
    Many industrial sectors, from pharmaceuticals to consumer products, are required to provide data on their products to demonstrate their efficacy and that they are safe for patients, consumers and the environment. This period of testing typically requires the use of animal models, the validity of which has been called into question due to the high rates of attrition across many industries. There is increasing recognition of the limitations of animal models and demands for safety and efficacy testing paradigms which embrace the latest technological advances and knowledge of human biology. Read More

    Mussel-inspired nano-building block assemblies for mimicking extracellular matrix microenvironments with multiple functions.
    Biofabrication 2017 Aug 3;9(3):035005. Epub 2017 Aug 3.
    Key Lab of Advanced Technologies of Materials, Ministry of Education, School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu, Sichuan, 610031, People's Republic of China.
    The assembly of nano-building blocks is an effective way to produce artificial extracellular matrix microenvironments with hierarchical micro/nano structures. However, it is hard to assemble different types of nano-building blocks, to form composite coatings with multiple functions, by traditional layer-by-layer (LbL) self-assembly methods. Inspired by the mussel adhesion mechanism, we developed polydopamine (PDA)-decorated bovine serum albumin microspheres (BSA-MS) and nano-hydroxyapatite (nano-HA), and assembled them to form bioactive coatings with micro/nano structures encapsulating bone morphogenetic protein-2 (BMP-2). Read More

    Bioprinting of a functional vascularized mouse thyroid gland construct.
    Biofabrication 2017 Aug 18;9(3):034105. Epub 2017 Aug 18.
    Laboratory for Biotechnological Research '3D Bioprinting Solutions', Moscow 115409, Russia.
    Bioprinting can be defined as additive biofabrication of three-dimensional (3D) tissues and organ constructs using tissue spheroids, capable of self-assembly, as building blocks. The thyroid gland, a relatively simple endocrine organ, is suitable for testing the proposed bioprinting technology. Here we report the bioprinting of a functional vascularized mouse thyroid gland construct from embryonic tissue spheroids as a proof of concept. Read More

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